Leica Aperio AT2 DX User manual

Aperio AT2 DX
MAN-0351, Revision B | 13 April 2020
User’s Guide

Aperio AT2 DX User’s Guide, Revision B © Leica Biosystems Imaging, Inc. 20202
Aperio AT2 DX User’s Guide
Copyright Notice
ÌCopyright © 2019-2020 Leica Biosystems Imaging, Inc. All Rights Reserved. LEICA and the Leica logo are registered trademarks of Leica
Microsystems IR GmbH. Aperio is a trademark of the Leica Biosystems group of companies in the USA and optionally in other countries. Other
logos, product and/or company names might be trademarks of their respective owners.
ÌThis product is protected by registered patents. For a list of patents, contact Leica Biosystems.
Customer Resources
ÌFor the latest information on Leica Biosystems Aperio products and services, please visit www.LeicaBiosystems.com/Aperio.
Disclaimers
ÌUse normal care in maintaining and using your MDDS. Interrupting network connections or turning off the MDDS while it is writing scanned
images can result in data loss.
ÌCaution: Federal (U.S.) law restricts this device to sale by or on the order of an appropriately licensed healthcare practitioner.
ÌThis manual is not a substitute for the detailed operator training provided by Leica Biosystems Imaging or for other advanced instruction. Leica
Biosystems Imaging Field Representatives should be contacted immediately for assistance in the event of any instrument malfunction. Installation
of hardware should only be performed by a certified Leica Biosystems Imaging Service Engineer.
ÌThe barcode decoding software used by Aperio scanners was provided by Honeywell International, Inc.
Contact Information – Leica Biosystems Imaging, Inc.
Headquarters Customer Support General Information
Leica Biosystems Imaging, Inc.
1360 Park Center Drive
Vista, CA 92081
USA
Tel: [phone] (toll free)
Direct International Tel: [phone]
Contact your local support representative
with any query and service request.
https://www.leicabiosystems.com/service-
support/technical-support/
US/Canada Tel: [phone] (toll free)
Direct International Tel: [phone]
Email: [email protected]
23AT2DX

Aperio AT2 DX User’s Guide, Revision B © Leica Biosystems Imaging, Inc. 2020 3
Customer Service Contacts
Please contact the office for your country for technical assistance.
Australia:
96 Ricketts Road
Mount Waverly, VIC 3149
AUSTRALIA
Tel: [phone] (toll free)
Between 8:30 AM-5 PM, Monday-Friday, AEST
Email: [email protected]
Austria:
Leica Biosystems Nussloch GmbH
Technical Assistance Center
Heidelberger Strasse 17
Nussloch 69226
GERMANY
Tel: [phone] (toll free)
In-country Tel: [phone]
Email: [email protected]
België/Belgique:
Tel: [phone] (toll free)
In-country Tel: [phone]
Email: [email protected]
Canada:
Tel: [phone] (toll free)
Direct International Tel: [phone]
Email: T[email protected]
China:
17F, SML Center No. 610 Xu Jia Hui Road, Huangpu District
Shanghai, PRC PC:200025
CHINA
Tel: [phone]
Fax: [phone]
Email: [email protected]
Remote Care email: [email protected]
Danmark:
Tel: [phone] (toll free)
In-country Tel: [phone]
Email: [email protected]
Deutschland:
Leica Biosystems Nussloch GmbH
Technical Assistance Center
Heidelberger Strasse 17
Nussloch 69226
GERMANY
Tel: [phone] (toll free)
In-country Tel: [phone]
Email: [email protected]
Eire:
Tel: [phone] (toll free)
In-country Tel: [phone]
Email: [email protected]
España:
Tel: [phone] (toll free)
In-country Tel: [phone]
Email: [email protected]
France:
Tel: [phone] (toll free)
In-country Tel: [phone]
Email: [email protected]
Italia:
Tel: [phone] (toll free)
In-country Tel: [phone]
Email: [email protected]
Japan:
1-29-9 Takadannobaba, Sinjuku-ku
Tokyo 169-0075
JAPAN
Nederland:
Tel: [phone] (toll free)
In-country Tel: [phone]
Email: [email protected]

Aperio AT2 DX User’s Guide, Revision B © Leica Biosystems Imaging, Inc. 20204
New Zealand:
96 Ricketts Road
Mount Waverly, VIC 3149
AUSTRALIA
Tel: [phone] (toll free)
Between 8:30 AM-5 PM, Monday-Friday, AEST
Email: [email protected]
Portugal:
Tel: [phone] (toll free)
In-country Tel: [phone]
Email: [email protected]
The Russian Federation
BioLine LLC
Pinsky lane 3 letter A
Saint Petersburg 197101
THE RUSSIAN FEDERATION
Tel: [phone] (toll free)
In-country Tel: [phone]
Email: [email protected]
Sweden:
Tel: [phone] (toll free)
In-country Tel: [phone]
Email: [email protected]
Switzerland:
Tel: [phone] (toll free)
In-country Tel: [phone]
Email: [email protected]
United Kingdom:
Tel: [phone] (toll free)
In-country Tel: [phone]
Email: [email protected]
USA:
Tel: [phone] (toll free)
Direct International Tel: [phone]
Email: T[email protected]

Aperio AT2 DX User’s Guide, Revision B © Leica Biosystems Imaging, Inc. 2020 5
1 Introduction ...................................................................................................................... 8
Intended Use...................................................................................................................................9
Image Viewing Restrictions for Primary Diagnosis....................................................................................9
If You Need Help..............................................................................................................................9
Symbols and Warnings....................................................................................................................10
Instrument Warnings....................................................................................................................... 11
Compliance Specifications................................................................................................................ 11
Installation ................................................................................................................................... 11
2 Startup and Shutdown................................................................................................... 12
System Startup..............................................................................................................................12
System Shutdown ..........................................................................................................................12
Calibration and Verification...............................................................................................................13
3 Preparing for Scanning ................................................................................................. 14
Training........................................................................................................................................14
Slide Preparation Tips......................................................................................................................14
Tissue Preparation ......................................................................................................................14
Staining....................................................................................................................................14
Slide Preparation ........................................................................................................................14
Coverslips .................................................................................................................................15
Label Issues ..............................................................................................................................15
Glass Slide Quality Control ...............................................................................................................16
4 Scanning Slides ............................................................................................................. 17
Scanner Controls............................................................................................................................17
Scanning Parameters ......................................................................................................................18
Starting the Console .......................................................................................................................18
Barcode Requirement......................................................................................................................18
Manually Entering a Barcode.........................................................................................................19
Multiple Slide Scanning Using the AutoLoader......................................................................................19
Replacing a Rack ........................................................................................................................19
Contents