Endress+Hauser analytikjena qTOWER3 84 auto User manual

Operating Manual
qTOWER³84/qTOWER³84G
Real-TimePCRThermal Cycler

Manufacturer
Analytik Jena GmbH+Co. KG
Konrad-Zuse-Straße 1
07745 Jena / Germany
Phone: [phone]
Fax: [phone]
Email: [email protected]
Technical Service Biometra GmbH
Rudolf-Wissell-Straße 30
37079 Göttingen / Germany
Phone: [phone]
Fax: + 49 551 50 68 666
Email: [email protected]
General Information http://www.analytik-jena.com
Documentation Number 10-3107-072-23
Edition D(05/2023)
Technical Documentation Analytik Jena GmbH+Co. KG
© Copyright 2023, Analytik Jena GmbH+Co. KG
For a proper and safe use of this product follow the instructions. Keep the
operating manual for future reference.

qTOWER³84/qTOWER³84G Table of contents
3
Table of contents
1 Notes on this operating manual........................................................................................................................... 5
2 Intended use......................................................................................................................................................... 6
3 Safety instructions................................................................................................................................................ 8
3.1 Safety markings ................................................................................................................................................ 8
3.2 Requirements for the operating personnel..................................................................................................... 8
3.3 Safety instructions– transport and installation.............................................................................................. 9
3.4 Safety instructions – operation........................................................................................................................ 9
3.4.1 General safety instructions for operation........................................................................................................ 9
3.4.2 Safety instructions – protection against explosion and fire........................................................................... 10
3.4.3 Safety instructions – electrical equipment ...................................................................................................... 10
3.4.4 Handling of auxiliary and operating materials and samples ......................................................................... 10
3.5 Safety instructions – maintenance and repair ................................................................................................ 11
3.6 Behavior during emergencies .......................................................................................................................... 11
4 Design and function.............................................................................................................................................. 12
4.1 Design, connections and control elements ..................................................................................................... 12
4.2 Function ............................................................................................................................................................ 14
4.2.1 Fluorescence spectrometer .............................................................................................................................. 14
4.2.2 PCR thermocycler.............................................................................................................................................. 16
4.2.3 Heated lid.......................................................................................................................................................... 16
4.2.4 Plastic ................................................................................................................................................................ 16
4.3 Type plate.......................................................................................................................................................... 17
5 Installation............................................................................................................................................................ 18
5.1 Installation location requirements................................................................................................................... 18
5.1.1 Spatial requirements ........................................................................................................................................ 18
5.1.2 Power supply..................................................................................................................................................... 18
5.2 Installation ........................................................................................................................................................ 19
6 Operation.............................................................................................................................................................. 21
6.1 Switching the device on and off....................................................................................................................... 21
6.2 Starting a real-time PCR analysis .................................................................................................................... 21
7 Error messages ..................................................................................................................................................... 23
8 Maintenance and care.......................................................................................................................................... 24
8.1 Cleaning the housing........................................................................................................................................ 24
8.2 Cleaning the sample block ............................................................................................................................... 25
8.3 Disinfecting the device ..................................................................................................................................... 25
8.4 Replacing fuses ................................................................................................................................................. 26
8.5 Installing color modules ................................................................................................................................... 27
9 Transport and storage.......................................................................................................................................... 31
9.1 Transport........................................................................................................................................................... 31
9.1.1 Inserting the transport lock.............................................................................................................................. 31
9.1.2 Return................................................................................................................................................................ 32

Table of contents qTOWER³84/qTOWER³84G
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9.1.3 Moving the device in the laboratory................................................................................................................ 32
9.2 Storage .............................................................................................................................................................. 33
10 Disposal ................................................................................................................................................................ 34
11 Specifications........................................................................................................................................................ 35
11.1 Technical data................................................................................................................................................... 35
11.2 Ambient conditions .......................................................................................................................................... 37
11.3 Standards and directives .................................................................................................................................. 37

qTOWER³84/qTOWER³84G Notes on this operating manual
5
1 Notes on this operating manual
This operating manual describes the following thermocycler models:
¡qTOWER³84
¡qTOWER³84 G
In the text below, these devices are collectively called qTOWER³84, or simply 'device'.
Differences between the models are explained in the corresponding section.
The device is intended for operation by qualified specialist personnel observing this user
manual.
The operating manual describes the design and function of the device, and and provides
personnel familiar with PCR technology with the necessary know-how for the safe han-
dling of the equipment. Furthermore, this operating manual provides information on
system maintenance and care, and on sources of potential faults or malfunctions and
how to remedy them.
Conventions Instructions for actions occurring in chronological order are numbered and combined
into action units.
Warnings are indicated by a warning triangle and a signal word. The type, source and
consequences of the hazard are stated together with notes on preventing the hazard.
Elements of the control and analysis program are indicated as follows:
¡Program terms are in bold (e.g., the System menu).
¡Menu items are separated by vertical lines (e.g., System|Device).
Symbols and signal words used
in this manual
The user manual uses the following symbols and signal words to indicate hazards or in-
structions. These warnings are always placed before an action.
WARNING
Indicates a potentially hazardous situation which can cause death or very serious (possi-
bly permanent) injury.
CAUTION
Indicates a potentially hazardous situation which can cause slight or minor injuries.
NOTICE
Provides information on potential material or environmental damage.

Intended use qTOWER³84/qTOWER³84G
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2 Intended use
NOTICE
The device is intended for general laboratory use.
The device may only be used for the applications described in these operating instruc-
tions.
The manufacturer does not accept liability for any other use.
The device is a thermocycler which is licensed for real-time PCR experiments that am-
plify DNA via polymerase chain reaction (PCR) while using fluorescence spectroscopy for
the highly sensitive detection of target sequences. The signal from the fluorescent dyes
excited by a light source correlates quantitatively with the amount of PCR product and
may be shown in real-time.
The integrated detector allows measurement of the sample flourescence in up to six
spectral channels during PCR, providing verification of multiple target sequences in a
single PCR reaction. The filters used on the color or FRET modules are precisely tuned to
the properties of the fluorescent dye most often used, allowing sensitive and selective
detection of fluorescent PCR products.
Analytik Jena offers a series of color or FRET modules, with up to six being able to be si-
multaneously installed in the device.
The replacement and retrofit of color or FRET modules is easy.
The device is an open platform for real-time PCR and supports both intercalating dyes as
well as individual sensors and kits from various manufacturers. The device can be used
in various applications, such as expression analyses, genotyping and the detection of
pathogens.
The device is fully controlled from the PC using the qPCRsoft384 software. The software
provides the following functions:
¡Device control and monitoring
¡Context-sensitive help functions
¡Design of real-time PCR experiments and their evaluation
¡Storage of methods (templates) and measuring results (projects)
¡User management
¡Planning and evaluation of
– Absolute quantifications
– Relative quantifications
– ΔΔCt analyses
– DNA melting curves
– Genotyping
– End point analyses
¡Results export to MS EXCEL or as CSV file
¡Results printout
¡Results export to extended programs for the analysis of real-time PCR data (e.g.
GenEx, qBASE)
A detailed description of the software can be found in the software manual.
We recommend the following scientific publication for an intensive introduction into the
real-time PCR analysis techniques and applications:

qTOWER³84/qTOWER³84G Intended use
7
LOGAN, Julie; EDWARDS, Kristin; SAUNDERS, Nick (ed.): Real-Time PCR – Current Tech-
nology and Application. Norfolk UK: Caister Academic Press, 2009

Safety instructions qTOWER³84/qTOWER³84G
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3 Safety instructions
For your own safety and to ensure error-free and safe operation of the device, please
read this chapter carefully before commissioning.
Observe all safety instructions listed in this user manual and all messages and displayed
by the control and analysis software on the monitor.
3.1 Safety markings
Warning and mandatory action labels have been attached to the device and must always
be observed.
Damaged or missing warning and mandatory action labels can cause incorrect actions
leading to personal injury or material damage. The labels must not be removed. Dam-
aged warning and mandatory action labels must be replaced immediately!
The following warning and mandatory action labels have been attached to the device:
Warning/mandatory sign Meaning
Disconnect the power supply before opening the
device cover.
The device contains controlled substances. Ana-
lytik Jena GmbH+Co. KG warrants that these sub-
stances will not be released from the device
within the next 25years provided the device is
employed as intended.
General warning sign
Warning about hot surface
Further symbols can be found on the type plate (→ "Type plate" 17).
3.2 Requirements for the operating personnel
The device must only be operated by qualified specialist personnel instructed in the use
of the device. The instruction also includes imparting the contents of this operating
manual.
In addition to the safety at work instructions in this operating manual the generally ap-
plicable safety and accident prevention regulations of the respective country of opera-
tion must be observed and adhered to. The operator must ensure the latest version of
these regulations.
¡The operating manual has to be accessible to the operating and maintenance per-
sonnel at all times!
¡Only authorized personnel may work with the device. The operating personnel must
be familiar with the dangers arising from samples and excipients. Use appropriate
personal protection.

qTOWER³84/qTOWER³84G Safety instructions
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¡When using the device, observe the appropriate laboratory diligence and cleanliness
to prevent contamination of the device. This reduces the risk of the user being con-
taminated with potentially infectious material and the risk of cross-contamination of
the samples. Use protective gloves and use other protective measures if skin contact
with infectious materials can occur during use of the device.
¡Decontaminate the device if the housing or the sample block has become contami-
nated with hazardous substances. Suitable disinfection agents and procedures are
described in "Disinfecting the device" (→ "Disinfecting the device" 25).
NOTICE
Other disinfection agents than those listed may only be used after consultation with An-
alytik Jena.
3.3 Safety instructions– transport and installation
The device may only be transported with the transport locks in place and in its original
packaging. Always ensure that the device is empty and no sample vessels are in the
sample block. More information can be found in the corresponding chapter of this oper-
ating manual.
The device can be installed by Analytik Jena customer service or trained specialist per-
sonnel authorized by Analytik Jena.
Observe the guide values and adhere to the legally mandated limits for lifting and carry-
ing without auxiliary means!
¡For safety reasons, 2 persons are required to transport the device.
¡Since the device does not have handles, grip the device firmly with both hands at
the bottom and lift it at the same time.
¡The installation location and the space required must be in accordance with specifi-
cations.
3.4 Safety instructions – operation
3.4.1 General safety instructions for operation
The operator must make sure that the device and its safety equipment is in sound condi-
tion each time before starting up the device. The technical condition must always com-
ply with the legal requirements and regulations.
¡Ensure easy access to the main power switch on the rear of the device housing dur-
ing operation.
¡The ventilation fittings at the rear and the bottom of the device must be free and
operational. Covered ventilation grilles or slots etc. may cause the device to break
down or may cause damage to it.
¡Ensure that the lid is securely closed before starting the program! Do not touch the
heated lid!
¡Be careful to avoid crushing or pinching injuries when closing the device.
¡The thermal block, the samples, and the heated lid reach high temperatures.
There is a risk of burns during contact.
¡Wear safety goggles during operation!
The rapid heating of the thermal block can cause liquids to evaporate explosively.
¡Only use plates, tubes, foils and caps suitable for high temperatures (up to 110°C)!

Safety instructions qTOWER³84/qTOWER³84G
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¡Do not touch hot sample tubes or plates and do not open them or boiling liquid may
escape!
3.4.2 Safety instructions – protection against explosion and fire
¡The device must not be operated with flammable, explosive or volatile substances.
¡The device may not be operated in an explosion-hazard environment.
3.4.3 Safety instructions – electrical equipment
The device is in accordance with the interference emission and interference immunity
requirements of the corresponding standards.
¡The device may only be connected to power sources whose nominal voltage is the
same as that on the rating plate of the device.
¡The electrical components must be checked regularly by a qualified electrician. Any
defects, such as loose connections or faulty/damaged cables must be repaired im-
mediately by specialist personnel.
¡In case of malfunctions of the electrical components, switch off the device at the
main switch immediately and disconnect the mains plug from the grid.
¡Before opening, the device must be isolated from all circuits!
¡Work on the electrical components of the device may only be performed by Analytik
Jena customer service employees and specially authorized technicians in accordance
with valid electrotechnical regulations. Life-threatening voltages may be applied
within the device! There is a risk of electric shock if contact is made with live compo-
nents, which may lead to serious injury or death.
¡Any work on the interior of the device, except for that described in this operating
manual, may only be carried out by Analytik Jena customer service and specially au-
thorized technicians.
¡Make sure the correct fuses are used and replace these if necessary. To do this, sep-
arate the device from the mains supply.
¡The device may only be used with the supplied power cable or a power cable with
the same specification (1.5m length, shielded, with grounding conductor). Exten-
sion of the power cable used is not permitted. The use of different power cables is
not permitted, and can result in increased electromagnetic interference emission or
decreased electromagnetic interference immunity of the device and incorrect device
operation.
¡Wipe spilled samples or reagents immediately with an absorbent cloth or piece of
paper. Do not allow any liquid to enter the device.
¡Do not use the device in environments with extreme humidity (>95%), or in loca-
tions in which condensation may occur.
3.4.4 Handling of auxiliary and operating materials and samples
The operator is responsible for the selection of substances used in the process, as well as
for their safe handling. This applies in particular for radioactive, pathogenic, infectious,
poisonous, corrosive, flammable, explosive or otherwise hazardous materials. For de-
tails, contact the safety officer responsible for your location.
¡In general, always wear safety goggles when handling reagents.
¡For your own safety, please observe the potential infectious qualities of the exam-
ined biological material.
¡Observe all notices on the cleaning and decontamination of the device. The use of
other cleaning or decontamination procedures is only permitted following prior con-
sultation with Analytik Jena.

qTOWER³84/qTOWER³84G Safety instructions
11
3.5 Safety instructions – maintenance and repair
Maintenance of the device is always carried out by the Analytik Jena customer service
department or specialist personnel trained and authorized by Analytik Jena. Unautho-
rized maintenance can damage the device.
The operator may only carry out the tasks listed in the chapter "Maintenance and care".
¡The exterior of the device may only be cleaned with a damp, not dripping, cloth af-
ter the device has been switched off.
¡Do not use alcohol (e.g., methanol or ethanol), organic solvents or abrasives to
clean the device.
¡Generally, all service and repair work on the device must be carried out in switched-
off condition (unless stated otherwise).
¡Use only original spare parts, wear parts and consumables. They have been tested
and ensure safe operation.
3.6 Behavior during emergencies
If there is no immediate risk of injury, switch off the device and the connected system
components immediately in hazardous situations or in the event of an accident and/or
disconnect the power plugs from the power outlets.

Design and function qTOWER³84/qTOWER³84G
12
4 Design and function
4.1 Design, connections and control elements
The device combines a PCR thermocycler with a patented fluorescence photometer.
The lock with handle and the LED for status display are located on the front side of the
device.
1
2
3
4
Fig.1 Front view
1 Upper part with fluorescence spectrom-
eter
2 Lock with handle
3 LED for status display 4 Lower part with thermocycler
The device is opened by folding back the upper part and the cover for the sample block
and fluorescence photometer it contains. To this end press the handle in until the lock
disengages with a click and the upper part gently snaps open. The upper part can then
be folded back from the handle.
Fig.2 Open device
1 Heated lid 2 Sample block
3 Locking pins

qTOWER³84/qTOWER³84G Design and function
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The mains connection and the power switch are located on the device rear.
The interface for connection to the PC is also located on the device rear.
1
2
3
456
7
8
Fig.3 Back
1 Ventilation for fluorescence spectrome-
ter
2 Ventilation grille on the thermal cycler
3 Service connection 4 Power connection
5 Fuse holder for device fuses 6 Mains switch
7 Type plate 8 Interface for the connection cable to the
connected PC
The voltage selector switch is located on the bottom of the device, behind a cover. It can
be used to adjust the operating voltage to the mains voltage.
Fig.4 Voltage selection switch on the bottom of the device
The following accessories are included in the delivery scope of the device:
¡Power cable
¡Connection cable for connecting to the PC
¡CD or USB stick with qPCRsoft384 software, with manual for software and device
¡Operating manual and software manual (print version)
¡Packaging and packaging instructions

Design and function qTOWER³84/qTOWER³84G
14
Fig.5 Mains and connection cable
Only use the mains cable supplied or a mains cable with the same specifications.
4.2 Function
4.2.1 Fluorescence spectrometer
The detector unit for a thermal block with 384 wells is a patented 16-channel epi-fluo-
rescence photometer with fiber multiplexer and a mechanical scanning device.
The following schematic illustration shows the components of the flourescence spec-
trometer:
Detector
Electronic unit
Multi-chip LED light source
Multiplexer
Measuring head above
thermal block PMT
DCU (CPU)
USB RS232 Energy
PCR thermoblock
Scan fibers
Optical fiber bundle
Heated lid
Color modules
Stepper
motor
Spindle
motor
Spindle
Lenses in the
measuring
head
Fig.6 Schematic representation of the fluorescence spectrometer
Light source A long-life, sturdy four-color LED (blue, green, white and red) in the device is used as an
excitation light source for the emission of fluorescent dyes. The LEDs allow for sensitive
excitation of various dyes across a very broad wavelength spectrum that goes deep into
red, with the light source not requiring any warm-up period.
Multiplexer The light is passed through optic fibers to collimator lenses, bundled and then trans-
ferred to the excitation filter of the color modules fitted to a rotating filter wheel. The
light is deflected via a beam divider and passed through additional optical fibers to a
lens array in a shuttle system scanning the sample block in columns.

qTOWER³84/qTOWER³84G Design and function
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Measuring head The fluorescent dyes are specifically excited in the reaction mix thanks to the light and
emit light of a longer wavelength. Using the lenses in the shuttle system, the emitted
light is bundled and passed back to the color modules via the optical fibers.
Photomultiplier (PMT) In the color modules, the light passes the beam divider followed by two emission filters
and is then transferred to the photomultiplier (CPM) for detection.
The following illustration schematically displays the beam path of the light starting at
the light source via the blue and green arrows.
1
2
3
4
5
6
7
8
9
10
Fig.7 Schematic representation of the light beam path through a filter color
module
1 Aspherical lens 2 Excitation filter
3 Collimator lens 4 Light source
5 Aspherical lens 6 Detector
7 Emission filter (glass) 8 Emission filter (interference)
9 Beam splitter 10 Sample
The filter wheel of the photometer can be populated freely with a choice of filter mod-
ules. The product portfolio of Analytik Jena comprises a total of 12 different filter mod-
ules (6 color modules for the most frequently used fluorescent dyes, from the blue to the
red excitation range; 5 filter modules specially optimized for FRET applications and 1
protein module for melting curve analysis).
In addition, filter modules can be retrofitted at any time, extending the application spec-
trum of the device.

Design and function qTOWER³84/qTOWER³84G
16
4.2.2 PCR thermocycler
The thermal block with 384 wells is made of aluminum that conducts heat very well.
This achieves a high temperature homogeneity and uniformity in combination with
heating rates of up to 4°C/s and cooling rates of up to 2°C/s.
This powerful thermal block is suitable for high-throughput applications in particular.
The G model variant is additionally equipped with a gradient function, and is particularly
suited for establishing new primary pairs.
The thermal blocks are perfectly sealed to prevent condensed water from penetrating
the Peltier elements underneath the sample block and other parts of the electronics.
This protects the Peltier elements and prolongs the service life of the device.
4.2.3 Heated lid
The device comes equipped with an automated heated lid. This can be set to
30to110°C and prevents condensation forming in the area of the reaction tubes above
the block surface level. Furthermore, the heated lid guarantees – regardless of the con-
sumables used – a reliable contact between the reaction tubes and the thermal block
during the entire real-time PCR run thanks to a constant contact pressure. This signifi-
cantly improves temperature uniformity.
4.2.4 Plastic
Sample block in 384-well SBS format are only suitable for use with standard PCR plates
with 384 wells.
These and other consumables can be ordered from Analytik Jena.
For real-time PCR applications, it is important that the sample carriers in plate format
are sealed with optically transparent foil (sealing foil) before the PCR run. 0.2mL indi-
vidual tubes and 8-well strips must be sealed with the corresponding suitable optical
lids.
NOTICE!The optical transparency of the foils affects the fluorescence signal directly.
For this reason, only use clear adhesive foil such as that provided for real-time PCR.
Regardless of the sealing method used, thanks to the lid technology, the same pressure
is always applied to the consumables for absolutely reproducible conditions.
The device is not limited to specific detection reagents or the plastic products of a spe-
cific manufacturer.

qTOWER³84/qTOWER³84G Design and function
17
4.3 Type plate
The type plate is located on the rear of the device. It provides the following information
shown in the figure:
¡Manufacturer and address
¡Protection class of the housing
¡Safety symbols (Caution: Observe the accompanying documents!
¡Device number
¡Year of manufacture
¡Disposal instructions (Do not dispose of as domestic waste!)
¡Conformity and test sign
¡Electrical connection data
¡Serial number
¡Order number
¡Device type and model

Installation qTOWER³84/qTOWER³84G
18
5 Installation
5.1 Installation location requirements
Ambient conditions The climate conditions for the installation location are listed in the technical specifica-
tions (→ "Ambient conditions" 37). If required, ensure that the room is tempera-
ture-controlled.
Installation location require-
ments
¡This laboratory device is designed for indoor use.
¡Do not use the device in wet and damp environments. Keep the device surface clean
and dry.
¡Avoid direct sunlight and radiation from heaters onto the device. If necessary, pro-
vide air conditioning.
¡Place the device on a heat-resistant and acid-resistant surface.
¡Do not locate the device near sources of electromagnetic interference.
¡Avoid mechanical shocks and vibrations.
¡Do not use the device in explosion-hazard environments.
¡Place the device on a stable surface.
¡The installation site must be free of drafts, dust and caustic fumes.
¡Keep the ventilation slits free and do not obstruct them with other devices.
5.1.1 Spatial requirements
NOTICE
When opening the device, the upper device hood tilts back. Provide adequate space for
this.
The opened device has a spatial requirement of 70cmx27.5cmx50cm (HxWxD).
In addition, keep a safety distance of at least 10cm to other equipment or walls.
Additional space is required next to the device for the PC, monitor and possibly a printer.
The PC, monitor and printer may also be placed on a separate table.
5.1.2 Power supply
WARNING
Risk of electric shock!
The device may only be connected to a properly earthed power outlet in accordance with
the voltage specifications on the type plate.
The device operates on single-phase alternating current. Prior to making the connection
check that the voltage selection switch of the device is set to the correct value.
The device may only be used with the supplied power cable or a power cable with the
same specification (1.5m length, shielded, with grounding conductor).

qTOWER³84/qTOWER³84G Installation
19
Electrical connection require-
ments Operating voltage 100/115/230V(AC)
Line frequency 50to60Hz
Power consumption ≤850W
Device fuse 2x10AT/250V
5.2 Installation
WARNING
Risk of electric shock!
Check that the mains connection conditions match those indicated on the type plate on
the rear of the device.
Before connecting the device to the mains network set the correct operating voltage at
the voltage switch on the underside of the device.
NOTICE
Unsuitable packaging material may cause damage to the device! Keep the original pack-
aging and transport lock for subsequent transports.
The following steps are required during the installation of the device:
¡Installing the color module (if not already installed)
¡Checking the set operating voltage and correcting it if it does not match the voltage
supplied at the site
¡Connecting the device to the mains and the PC
¡Installing software on the PC
}Remove the device, the connection cables and the operating manual with the instal-
lation CD or USB stick from the transport packaging. Wait until the device has
reached room temperature for commissioning.
}Verify that the delivery is complete. Check the device and accessories for transport
damage.
In the event of an incomplete delivery or transport damage, please contact Analytik
Jena.
}Install the color modules if these are not already installed (→ "Installing color mod-
ules" 27).
}Set the operating voltage:
– Place the device on its side. Remove the cover from the voltage selection switch
on the bottom.
– Adjust the switch using a screwdriver or a coin so that the arrow points in the di-
rection of the mains voltage available on site.
– Replace the cover on the selection switch and put the device back on its feet.

Installation qTOWER³84/qTOWER³84G
20
Fig.8 Voltage selection switch on the bottom of the device
}Open the lid of the device. To do so, press in the red handle on the front until the
lock clicks open. Fold back the upper part of the device.
}Remove the transport lock and put it aside for later transports.
}Connect the connection cable to the device interface and to the PC.
}Connect the power cable to the device. Plug the plug into the mains socket.
NOTICE!Ensure that the power switch and the power cable can be easily accessed.
This is important in the event that the device needs to be disconnected from the supply
voltage.
}Switch on the PC. Install the software on the PC. Observe the information in the soft-
ware manual for this.
}Switch on the device via the power switch.
When first switched on, the device is detected as a connected device. After the driv-
ers install automatically, the device is ready for operation. If the drivers are not in-
stalled automatically, you can complete the installation via the Windows routine.
The drivers can be found on the installation CD or USB stick.
}Start the software.
}If color modules have been installed, specify these in the software.
üThe device is operational.
Operation is controlled via the software program. The settings for the measuring or
temperature program can be found in the software manual.