Millipore 8003 User manual

Stirred Ultraltration Cells
Models 8003, 8010, 8050, 8200, 8400
User Guide
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Notice
The information in this document is subject to change
without notice and should not be construed as a
commitment by Millipore Corporation or an afliated
corporation. Neither Millipore Corporation nor any of its
afliated corporations assumes responsibility for any
errors that may appear in this document. This manual
is believed to be complete and accurate at the time of
publication. In no event shall Millipore Corporation or an
afliated corporation be liable for incidental or conse-
quential damages in connection with or arising from the
use of this manual.
© 2008 MILLIPORE CORPORATION. PRINTED IN
THE UNITED STATES OF AMERICA. ALL RIGHTS
RESERVED.
Millipore and Amicon are registered trademarks of Millipore Corporation.
The M mark is a trademark of Millipore Corporation.
Cellosolve is a registered trademark of Union Carbide Chemicals &
Plastics Technology Corporation.
Tygon is a registered trademark of Saint-Gobain Performance Plastics
Corporation.
Saint-Gobain is a registered trademark of Compagnie de Saint-Gobain
Corporation.
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Contents
Introduction ............................................. 1
Models Available ..................................... 1
Safety Features ...................................... 1
Model 8003 Parts List ............................. 2
Model 8010 Parts List ............................. 4
Model 8050 Parts List ............................. 6
Model 8200 Parts List ............................. 8
Model 8400 Parts List ........................... 10
Operating Modes .................................. 12
Unpacking the System .......................... 13
Setting Up the System .......................... 14
Operating Guidelines ............................ 17
Limitations ............................................ 18
Cell Operation ....................................... 19
Shutting Down the System .................... 21
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Sterilization ........................................... 21
Storage ................................................. 22
Maintenance ......................................... 22
Troubleshooting .................................... 23
Specications ....................................... 24
Materials of Construction ...................... 24
Compliance with the Pressure Equipment
Directive, 97/23/EC ............................... 25
Product Ordering Information................ 26
Technical Assistance............................. 30
Standard Warranty ................................ 30
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Introduction
Millipore stirred ultraltration cells are designed for
rapid concentration or purication of macromolecular
solutions in volumes from 3 to 400 mL. All models are
used in conjunction with a stirring table. The table is
magnetically coupled to a stirring bar, which maintains
uid movement during operation, thereby reducing the
negative effects of concentration polarization (i.e., the
buildup of concentrated solutes on the membrane).
Models Available
Model Capacity Millipore Cat. No.
8003 3 mL 5125
8010 10 mL 5121
8050 50 mL 5122
8200 200 mL 5123
8400 400 mL 5124
Safety Features
Cell has a built-in pressure-relief valve, preset to ■
nominal 75 psi (5.3 kg/cm2). The recommended
operating pressure is <55 psi (3.9 kg/cm2).
Cell remains xed in the retaining stand until ■
the pressure-relief valve is opened and the cap
depressed.
Stand provides a large base for improved cell stability.■
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2www.millipore.com
Model 8003 Parts List
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3Stirred Ultraltration Cells
Model 8003 Parts List,continued
Item Description Quantity Supplied
A Cap Assembly 1
BPressure Relief Valve —
CO-Ring 2
DStirrer Assembly 1
EBody 1
FO-Ring 2
GMembrane —
HMembrane Holder 1
IElastomeric Tubing 4 in.
J Base 1
KTube Fitting Assembly 2
L Tubing, Plastic 4 ft
MStand Assembly 1
NO-Ring 2
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4www.millipore.com
Model 8010 Parts List
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5Stirred Ultraltration Cells
Model 8010 Parts List, continued
Item Description Quantity Supplied
A Cap Assembly 1
BPressure Relief Valve —
CO-Ring 2
DStirrer Assembly 1
EBody 1
FO-Ring 2
GMembrane —
HMembrane Holder 1
IElastomeric Tubing 4 in.
J Base 1
KTube Fitting Assembly 2
L Tubing, Plastic 4 ft
MStand Assembly 1
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6www.millipore.com
Model 8050 Parts List
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7Stirred Ultraltration Cells
Model 8050 Parts List, continued
Item Description Quantity Supplied
A Cap Assembly 1
BPressure Relief Valve —
CO-Ring 2
DStirrer Assembly 1
EBody 1
FO-Ring 2
GMembrane —
HMembrane Holder 1
IElastomeric Tubing 4 in.
J Base 1
KTube Fitting Assembly 2
L Tubing, Plastic 4 ft
MStand Assembly 1
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8www.millipore.com
Model 8200 Parts List
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9Stirred Ultraltration Cells
Model 8200 Parts List,continued
Item Description Quantity Supplied
A Cap Assembly 1
BPressure Relief Valve —
CO-Ring 2
DStirrer Assembly 1
EBody 1
FO-Ring 2
GMembrane —
HMembrane Holder 1
IElastomeric Tubing 4 in.
J Base 1
KTube Fitting Assembly 2
L Tubing, Plastic 4 ft
MStand Assembly 1
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10 www.millipore.com
Model 8400 Parts List
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11Stirred Ultraltration Cells
Model 8400 Parts List,continued
Item Description Quantity Supplied
A Cap Assembly 1
BPressure Relief Valve —
CO-Ring 2
DStirrer Assembly 1
EBody 1
FO-Ring 2
GMembrane —
HMembrane Holder 1
IElastomeric Tubing 4 in.
J Base 1
KTube Fitting Assembly 2
L Tubing, Plastic 4 ft
MStand Assembly 1
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12 www.millipore.com
Operating Modes
Stirred ultraltration cells can be operated in two
modes: concentration and dialtration.
Concentration
In this mode, apply gas pressure directly to the ultra-
ltration cell. Solutes above the membrane molecular
weight cut-off are retained in the cell, while water and
solutes below the cut-off pass into the ltrate and out of
the cell.
Dialtration
Connect the ultraltration cell to an auxiliary reservoir
(Millipore cat. no. 6028) containing a dialtration
solution with the desired microsolute concentration.
The use of stirred cells in the dialtration mode also
requires use of the CDS-10 Concentration/Dialysis
Selector (Milipore cat. no. 6003). The combination of
the auxiliary reservoir and the CDS-10 selector form
a system that keeps the stirred cell uid volume and
macrosolute concentration constant as the ltrate
volume is replaced by the dialtration solution. This
technique provides a simple means for rapid microso-
lute exchange, and is typically used as a substitute for
dialysis.
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13Stirred Ultraltration Cells
Unpacking the System
Place cell on a level surface and remove packing
inserts. Turn the pressure relief valve knob to the hori-
zontal position. Hold the body and push the cap down.
Slide the cell out of the retaining stand and remove the
cap assembly with a twisting motion. Pull out the stirrer
assembly and discard packing. Unscrew the base and
remove the membrane holder. Remove the O-ring from
the membrane holder.
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14 www.millipore.com
Setting Up the System
To identify the parts of the stirred cell, refer to the
diagrams in the appropriate model parts list section.
This section explains how to install the membrane and
stirring assembly.
Membrane Installation
NOTE: Handle the membrane by its edges, to avoid
scratching or contaminating the surface.
Model 8003 only:1.
Invert the cell body and place the smaller membrane
holder O-ring (Model 8003 Parts List — N) into the
recess. Gently tap to ensure that the O-ring remains
when the body is upright. Place the membrane into
the holder, shiny side up. Then place the larger
membrane holder O-ring (Model 8003 Parts List — F)
down, so that it contacts and seats the membrane
evenly in the bottom of the holder.
All other models (8010, 8050, 8200, and 8400):
Place the membrane into the holder, shiny side up.
Place the O-ring on top of the membrane. Gently
push the O-ring down, so that it contacts and seats
the membrane evenly in the bottom of the holder.
Fit the membrane holder into the cell body, aligning 2.
the tabs on the sides of the holder with the slots in
the base of the cell body.
Invert the cell body and membrane holder; screw the 3.
base rmly into the bottom of the cell body.
Push the ltrate exit tubing onto the exit spout of the 4.
membrane holder.
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15Stirred Ultraltration Cells
Stirrer Assembly Installation
Place the stirrer assembly into the cell body. When 1.
properly installed, the arms of the stirrer assembly
will rest on the small ridge inside the top of the cell
body.
Pour the sample into the cell.2.
Push the cap assembly down onto the cell body, 3.
using a twisting motion and orienting the gas inlet
port on the cap opposite the ltrate exit port on the
holder.
NOTE: If the cap assembly does not slide easily,
lubricate the O-ring lightly with water or
petroleum jelly. To avoid clogging the
membrane pores, do not allow petroleum
jelly to contact the membrane.
Set the pressure-relief valve knob to the horizontal 4.
(open) position.
Slide the cell into the retaining stand, tting the ring 5.
on the cell base into the hole in the stand. The
attened edge on the bottom ange of the cap
ensures that the cell is inserted properly and
prevents rotation of the cell once inside the stand.
Turn the pressure-relief valve knob to the vertical 6.
(closed) position.
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16 www.millipore.com
Gas Pressure Line Installation
Attach the gas pressure line to the tube tting on the
cap as follows:
Unscrew the hexagonal nut (a) from the cap assem-1.
bly and remove the O-ring (d).
Slide the hex nut onto the end of gas tubing. 2.
Slide the metal grab ring (b) down one half-inch onto 3.
the tubing, with the ange facing away from the hex
nut.
Add the white spacer (c), positioning the counter-4.
sunk surface toward the ange on the grab ring.
Slide the O-ring (d) onto the end of the tubing. 5.
Insert the exposed tubing into the cap assembly and 6.
hand-tighten the hexagonal nut. Do not overtighten.
a
b
d
c
½”
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