Binder KBF P 240 Owner's manual

Issue 11/2016 Art. N0. 7001-0320
Operating Manual
Translation of the original operating manual
KBF P (E6)
Constant climate chambers with program control
and adjustable light cassettes
Model Model version Art. No.
KBF P 240 KBFP240-230V 9020-0328, 9120-0328
KBF P 240-UL KBFP240UL-240V 9020-0329, 9120-0329
KBF P 720 KBFP720-230V 9020-0330, 9120-0330
KBF P 720-UL KBFP720UL-240V 9020-0331, 9120-0331
KBF LQC (E6)
Constant climate chambers
with ICH compliant illumination and light dose detection
with program control and adjustable light cassettes
Model Model version Art. No.
KBF LQC 240 KBFLQC240-230V 9020-0332, 9120-0332
KBF LQC 240-UL KBFLQC240UL-240V 9020-0333, 9120-0333
KBF LQC 720 KBFLQC720-230V 9020-0334, 9120-0334
KBF LQC 720-UL KBFLQC720UL-240V 9020-0335, 9120-0335
KBWF (E6)
Growth chambers with light and humidity
with program control and adjustable light cassettes
Model Model version Art. No.
KBWF 240 KBWF240-230V 9020-0336, 9120-0336
KBWF 720 KBWF720-230V 9020-0337, 9120-0337
BINDER GmbH
Address: Post office box 102, 78502 Tuttlingen, Germany Phone: [phone]
Fax: [phone] Internet: http://www.binder-world.com
E-mail: [e-mail] Service Hotline: [phone]
Service Fax: [phone] Service E-Mail: [e-mail]
Service Hotline USA: [phone] or [phone] x3
Service Hotline Asia Pacific: [phone] or [phone]
Service Hotline Russia and CIS: [phone]

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Content
1. SAFETY..................................................................................................................7
1.1 Legal considerations ...........................................................................................................................7
1.2 Structure of the safety instructions......................................................................................................7
1.2.1 Signal word panel......................................................................................................................7
1.2.2 Safety alert symbol ....................................................................................................................8
1.2.3 Pictograms.................................................................................................................................8
1.2.4 Word message panel structure .................................................................................................9
1.3 Localization / position of safety labels on the chamber.......................................................................9
1.4 Type plate..........................................................................................................................................11
1.5 General safety instructions on installing and operating the chambers..............................................12
1.6 Intended use......................................................................................................................................14
1.7 Operating instructions .......................................................................................................................14
1.8 Measures to prevent accidents .........................................................................................................15
1.9 Resistance of the humidity sensor against harmful substances .......................................................16
2. CHAMBER DESCRIPTION AND OVERVIEW .....................................................17
2.1 Chamber description.........................................................................................................................17
2.1.1 KBF P / KBF LQC....................................................................................................................17
2.1.2 KBWF......................................................................................................................................17
2.1.3 General....................................................................................................................................18
2.2 Chamber overview ............................................................................................................................19
2.3 Instrument panel................................................................................................................................19
2.4 Lateral control panels........................................................................................................................20
2.5 Rear view with water connections.....................................................................................................21
3. COMPLETENESS OF DELIVERY, TRANSPORTATION, STORAGE, AND
INSTALLATION....................................................................................................22
3.1 Unpacking, and checking equipment and completeness of delivery.................................................22
3.2 Guidelines for safe lifting and transportation.....................................................................................23
3.3 Storage..............................................................................................................................................23
3.4 Location of installation and ambient conditions.................................................................................24
4. INSTALLATION AND CONNECTIONS................................................................26
4.1 Spacer for wall distance....................................................................................................................26
4.2 Wastewater connection.....................................................................................................................26
4.3 Freshwater supply.............................................................................................................................27
4.3.1 Automatic fresh water supply via water pipe ...........................................................................27
4.3.2 Manual fresh water supply via external freshwater can (option)..............................................28
4.3.3 Connection kit for connecting the chamber to the water main ................................................28
4.3.4 Safety kit: Hose burst protection device with reflux protection device (available via BINDER
INDIVIDUAL customized solutions)........................................................................................29
4.4 Installation and connection of the light cassettes..............................................................................31
4.5 Connecting the light sensors – KBF LQC..........................................................................................32
4.6 Electrical connection .........................................................................................................................33
4.7 Connection of the voltage changer (option for KBF P 240 / KBF LQC 240) .....................................34
5. FUNCTIONAL OVERVIEW OF THE MB2 CHAMBER CONTROLLER...............36
5.1 Operating functions in normal display...............................................................................................38
5.2 Display views: Normal display, program display, chart-recorder display...........................................39
5.3 Controller icons overview..................................................................................................................40
5.4 Operating modes...............................................................................................................................42
5.5 Controller menu structure..................................................................................................................43
5.5.1 Main menu...............................................................................................................................44
5.5.2 “Settings” submenu .................................................................................................................45
5.5.3 “Service” submenu ..................................................................................................................45

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5.6 Principle of controller entries.............................................................................................................46
5.7 Performance during and after power failures....................................................................................46
5.8 Performance when opening the door................................................................................................47
6. START UP............................................................................................................47
6.1 Turning on the chamber....................................................................................................................47
6.2 Controller settings upon start up .......................................................................................................48
6.3 Turning on/off humidity control..........................................................................................................49
7. FUNCTION OF LIGHT MEASUREMENT, AND INTEGRATION: LIGHT
QUANTUM CONTROL – KBF LQC.....................................................................50
7.1 Display of the instantaneous and the integrated values....................................................................50
7.2 Measurement of illumination intensity and temporal integration .......................................................50
8. SET-POINT ENTRY IN “FIXED VALUE” OPERATING MODE...........................52
8.1 Set-point entry through the “Setpoints” menu ...................................................................................53
8.2 Direct setpoint entry via Normal display............................................................................................54
8.3 Automatic correction of the actual value when turning on or off the illumination ..............................55
8.4 Light commutation and special controller functions via operation lines ............................................55
9. TIMER PROGRAM: STOPWATCH FUNCTION...................................................57
9.1 Starting a timer program ...................................................................................................................57
9.1.1 Performance during program delay time.................................................................................58
9.2 Stopping a running timer program.....................................................................................................58
9.2.1 Pausing a running timer program ............................................................................................58
9.2.2 Cancelling a running timer program ........................................................................................59
9.3 Performance after the end of the program........................................................................................59
10. TIME PROGRAMS ...............................................................................................60
10.1 Starting an existing time program .....................................................................................................60
10.1.1 Performance during program delay time.................................................................................61
10.2 Stopping a running time program......................................................................................................62
10.2.1 Pausing a running time program .............................................................................................62
10.2.2 Cancelling a running time program..........................................................................................62
10.3 Performance after the end of the program........................................................................................62
10.4 Creating a new time program............................................................................................................63
10.5 Program editor: program management.............................................................................................63
10.6 Section editor: Section management ................................................................................................65
10.6.1 Add a new section ...................................................................................................................66
10.6.2 Copy and insert or replace a section.......................................................................................66
10.6.3 Deleting a section....................................................................................................................68
10.7 Value entry for a program section.....................................................................................................69
10.7.1 Section duration.......................................................................................................................70
10.7.2 Set-point ramp and set-point step ...........................................................................................70
10.7.3 Light commutation and special controller functions via operation lines...................................72
10.7.4 Temperature setpoint ..............................................................................................................73
10.7.5 Humidity setpoint.....................................................................................................................74
10.7.6 Fan speed setpoint..................................................................................................................74
10.7.7 UVA target dose value – KBF LQC .........................................................................................75
10.7.8 VIS target dose value – KBF LQC...........................................................................................75
10.7.9 Tolerance ranges.....................................................................................................................76
10.7.10 Repeating one or several sections within a time program.......................................................77
10.7.11 Saving the time program .........................................................................................................78
11. WEEK PROGRAMS.............................................................................................79
11.1 Starting an existing week program....................................................................................................79
11.2 Cancelling a running week program..................................................................................................80
11.3 Creating a new week program ..........................................................................................................81
11.4 Program editor: program management.............................................................................................82

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11.5 Section editor: Section management ................................................................................................83
11.5.1 Add a new section ...................................................................................................................84
11.5.2 Copy and insert or replace a section.......................................................................................85
11.6 Value entry for a program section.....................................................................................................85
11.6.1 Set-point ramp and set-point step modes................................................................................85
11.6.2 Weekday..................................................................................................................................86
11.6.3 Start time .................................................................................................................................86
11.6.4 Temperature setpoint ..............................................................................................................87
11.6.5 Humidity setpoint.....................................................................................................................87
11.6.6 Fan speed setpoint..................................................................................................................88
11.6.7 UVA target dose value – KBF LQC .........................................................................................88
11.6.8 VIS target dose value – KBF LQC...........................................................................................89
11.6.9 Light commutation and special controller functions via operation lines...................................89
11.7 Deleting a week program ..................................................................................................................90
12. NOTIFICATION AND ALARM FUNCTIONS ........................................................91
12.1 Notification and alarm messages overview.......................................................................................91
12.1.1 Notifications.............................................................................................................................91
12.1.2 Messages when reaching a dose target value – KBF LQC.....................................................92
12.1.3 Alarm messages......................................................................................................................93
12.1.4 Messages concerning the humidity system.............................................................................93
12.2 State of alarm....................................................................................................................................94
12.3 Resetting an alarm, list of active alarms ...........................................................................................94
12.4 Tolerance range settings...................................................................................................................95
12.5 Activating / deactivating the audible alarm (alarm buzzer)................................................................96
13. TEMPERATURE SAFETY DEVICES...................................................................97
13.1 Over temperature protective device (class 1) ...................................................................................97
13.2 Overtemperature safety controller class 3.1 .....................................................................................97
13.2.1 Safety controller modes...........................................................................................................97
13.2.2 Setting the safety controller.....................................................................................................98
13.2.3 Message and measures in the state of alarm .........................................................................99
13.2.4 Function check ........................................................................................................................99
13.3 Temperature safety device class 3.3 (option).................................................................................100
13.3.1 Temperature safety device class 3.1.....................................................................................101
13.3.2 Temperature safety device class 3.2.....................................................................................102
14. USER MANAGEMENT.......................................................................................103
14.1 Authorization levels and password protection.................................................................................103
14.2 Log in...............................................................................................................................................106
14.3 Log out ............................................................................................................................................107
14.4 User change....................................................................................................................................107
14.5 Password assignment and password change.................................................................................108
14.5.1 Password change..................................................................................................................108
14.5.2 Deleting the password for an individual authorization level...................................................111
14.5.3 New password assignment for “service” or “admin” authorization level when the password
function was deactivated.......................................................................................................112
14.6 Activation code................................................................................................................................113
15. GENERAL CONTROLLER SETTINGS..............................................................114
15.1 Selecting the controller’s menu language .......................................................................................114
15.2 Selecting the temperature unit ........................................................................................................114
15.3 Setting date and time ......................................................................................................................115
15.4 Display configuration.......................................................................................................................117
15.4.1 Adapting the display parameters...........................................................................................117
15.4.2 Touchscreen calibration ........................................................................................................119
15.5 Network and communication...........................................................................................................120
15.5.1 Serial interfaces.....................................................................................................................120
15.5.2 Ethernet.................................................................................................................................121
15.5.3 Web server............................................................................................................................122

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15.5.4 E-Mail.....................................................................................................................................124
15.6 USB menu.......................................................................................................................................126
16. GENERAL INFORMATION ................................................................................127
16.1 Service contact page.......................................................................................................................127
16.2 Current operating parameters.........................................................................................................127
16.3 Event list..........................................................................................................................................128
16.4 Technical chamber information.......................................................................................................129
17. CHART RECORDER DISPLAY..........................................................................130
17.1 Views...............................................................................................................................................130
17.2 Setting the parameters....................................................................................................................134
18. HUMIDIFICATION / DEHUMIDIFICATION SYSTEM .........................................136
18.1 Function of the humidifying and dehumidifying system...................................................................138
19. DEFROSTING AT REFRIGERATING OPERATION..........................................139
20. ILLUMINATION SYSTEM...................................................................................140
20.1 ICH compliant illumination according to CPMP/ICH/279/95 (Q1B) – KBF P, KBF LQC.................140
20.2 Illumination for optimal plant growth – KBWF.................................................................................141
20.3 Adjustable light cassettes................................................................................................................141
20.4 Illumination control ..........................................................................................................................143
20.5 Characteristic features of the light sensors – KBF LQC..................................................................144
20.5.1 LUX sensor............................................................................................................................144
20.5.2 UVA sensor............................................................................................................................144
20.5.3 Spectral range .......................................................................................................................144
20.5.4 Spatial sensitivity ...................................................................................................................145
21. OPTIONS............................................................................................................147
21.1 Communication software APT-COM™ 3 DataControlSystem (option)...........................................147
21.2 RS485 interface (option) .................................................................................................................147
21.3 Data logger kits (option)..................................................................................................................147
21.4 Analog outputs for temperature and humidity (option)....................................................................148
21.5 Zero-voltage relay alarm outputs for temperature and humidity (option) ........................................148
21.6 Water protected internal socket (option).........................................................................................149
21.7 Additional flexible Pt 100 temperature sensor (available via BINDER INDIVIDUAL customized
solutions).........................................................................................................................................150
21.8 Object temperature display with flexible Pt 100 temperature sensor (option).................................150
21.9 External freshwater and wastewater cans (option) .........................................................................151
21.9.1 Mounting the freshwater can .................................................................................................151
21.9.2 Mounting the wastewater can................................................................................................153
21.9.3 Mounting with wastewater recycling ......................................................................................154
21.10 BINDER Pure Aqua Service (option)...............................................................................................155
22. MAINTENANCE, CLEANING, AND SERVICE...................................................155
22.1 Maintenance intervals, service........................................................................................................155
22.2 Replacement of the fluorescent tubes.............................................................................................156
22.3 Calibrating the light sensors and adjusting the controller display – KBF LQC ................................157
22.4 Cleaning and decontamination........................................................................................................157
22.4.1 Cleaning.................................................................................................................................157
22.4.2 Decontamination....................................................................................................................159
22.5 Sending the chamber back to BINDER GmbH ...............................................................................160
23. DISPOSAL..........................................................................................................160
23.1 Disposal of the transport packing....................................................................................................160
23.2 Decommissioning............................................................................................................................161
23.3 Disposal of the chamber in the Federal Republic of Germany........................................................161

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23.4 Disposal of the chamber in the member states of the EU except for the Federal Republic of
Germany..........................................................................................................................................162
23.5 Disposal of the chamber in non-member states of the EU .............................................................163
24. TROUBLESHOOTING .......................................................................................164
25. TECHNICAL DESCRIPTION..............................................................................167
25.1 Factory calibration and adjustment .................................................................................................167
25.2 Over current protection....................................................................................................................167
25.3 Definition of usable volume.............................................................................................................167
25.4 Technical Data ................................................................................................................................168
25.5 Equipment and options (extract) .....................................................................................................170
25.6 Spare parts and accessories (extract) ............................................................................................171
25.7 Dimensions size 240.......................................................................................................................173
25.8 Dimensions size 720.......................................................................................................................174
26. CERTIFICATES AND DECLARATIONS OF CONFORMITY .............................175
26.1 EU Declaration of Conformity for KBF P.........................................................................................175
26.2 EU Declaration of Conformity for KBF LQC....................................................................................177
26.3 EU Declaration of Conformity for KBWF.........................................................................................179
26.4 Certificate for the GS mark of conformity of the “Deutsche Gesetzliche Unfallversicherung e.V.“
(German Social Accident Insurance) DGUV...................................................................................181
27. PRODUCT REGISTRATION ..............................................................................183
28. CONTAMINATION CLEARANCE CERTIFICATE..............................................184
28.1 For chambers located outside USA and Canada............................................................................184
28.2 For chambers located in USA and Canada.....................................................................................187

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Dear customer,
For the correct operation of the chambers, it is important that you read this operating manual completely
and carefully and observe all instructions as indicated. Failure to read, understand and follow the instruc-
tions may result in personal injury. It can also lead to damage to the chamber and/or poor equipment per-
formance.
1. Safety
This operating manual is part of the components of delivery. Always keep it handy for reference. The de-
vice should only be operated by laboratory personnel especially trained for this purpose and familiar with
all precautionary measures required for working in a laboratory. Observe the national regulations on mini-
mum age of laboratory personnel. To avoid injuries and damage observe the safety instructions of the
operating manual.
WARNING
Failure to observe the safety instructions.
Serious injuries and chamber damage.
Observe the safety instructions in this operating manual.
Carefully read the complete operating instructions of the chambers.
1.1 Legal considerations
This operating manual is for informational purposes only. It contains information for installing, start-up,
operation and maintenance of the product. Note: the contents and the product described are subject to
change without notice.
Understanding and observing the instructions in this operating manual are prerequisites for hazard-free
use and safety during operation and maintenance. In no event shall BINDER be held liable for any dam-
ages, direct or incidental arising out of or related to the use of this manual.
This operating manual cannot cover all conceivable applications. If you would like additional information,
or if special problems arise that are not sufficiently addressed in this manual, please ask your dealer or
contact us directly by phone at the number located on page one of this manual
Furthermore, we emphasize that the contents of this operating manual are not part of an earlier or existing
agreement, description, or legal relationship, nor do they modify such a relationship. All obligations on the
part of BINDER derive from the respective purchase contract, which also contains the entire and exclu-
sively valid statement of warranty administration. The statements in this manual neither augment nor re-
strict the contractual warranty provisions.
1.2 Structure of the safety instructions
In this operating manual, the following safety definitions and symbols indicate dangerous situations follow-
ing the harmonization of ISO 3864-2 and ANSI Z535.6.
1.2.1 Signal word panel
Depending on the probability of serious consequences, potential dangers are identified with a signal word,
the corresponding safety color, and if appropriate, the safety alert symbol.
DANGER
Indicates an imminently hazardous situation that, if not avoided, will result in death or serious
(irreversible) injury.
WARNING
Indicates a potentially hazardous situation which, if not avoided, could result in death or serious
(irreversible) injury.

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CAUTION
Indicates a potentially hazardous situation which, if not avoided, may result in moderate or minor
(reversible) injury.
CAUTION
Indicates a potentially hazardous situation which, if not avoided, may result in damage to the product
and/or its functions or of a property in its proximity.
1.2.2 Safety alert symbol
Use of the safety alert symbol indicates a risk of injury.
Observe all measures that are marked with the safety alert symbol in order to avoid death or
injury.
1.2.3 Pictograms
Warning signs
Electrical hazard
Hot surface
Explosive atmosphere Stability hazard
Lifting hazard
Scalding hazard
High humidity
UV light hazard
Danger of frost
Risk of corrosion and /
or chemical burns
Harmful substances
Biohazard
Pollution Hazard
Mandatory action signs
Mandatory regulation
Read operating
instructions
Disconnect the power
plug
Lift with several persons
Lift with mechanical
assistance
Environment protection
Wear protective gloves
Wear safety goggles

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Prohibition signs
Do NOT touch Do NOT spray with
water
Do NOT climb
Information to be observed in order to ensure optimum function of the product.
1.2.4 Word message panel structure
Type / cause of hazard.
Possible consequences.
∅Instruction how to avoid the hazard: prohibition
Instruction how to avoid the hazard: mandatory action.
Observe all other notes and information not necessarily emphasized in the same way, in order to avoid
disruptions that could result in direct or indirect injury or property damage.
1.3 Localization / position of safety labels on the chamber
The following labels are located on the chamber:
Pictograms (warning signs)
Risk of injury (on outer door, only KBF-240V and KMF-240V).
Observe the safety instructions in the operating manual.
Hot surface (inner glass door above the glass door handle)
UV light hazard (on outer door, KBF P / KBF LQC only)
Burning and scalding hazard (chamber rear)
Service label

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Figure 1: Position of labels on the chamber front (example: KBFP-240V)
Figure 2: Position of labels on the chamber rear
Keep safety labels complete and legible.
Replace safety labels that are no longer legible. Contact BINDER Service for these replacements.

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1.4 Type plate
The type plate sticks to the left side of the chamber, bottom right-hand.
Figure 3: Type plate (example KBF P 240 regular chamber 9020-0328)
Indications of the type plate (example)
Information
BINDER
Manufacturer: BINDER GmbH
KBF P 240
Model designation
Constant climate chamber
Device name
Built
00000000000000
Serial no. of the chamber
Serial No.
2017
Year of construction
Nominal temperature
70 °C / 158 °F
Nominal temperature
IP protection
20
IP type of protection acc. to standard EN 60529
Temp. safety device DIN 12880
Temperature safety device acc. to standard DIN
12880:2007
Class
3.1
Class of temperature safety device
Art. No.
9020-0328
Art. no. of the chamber
Project No.
---
Optional: Special application acc. to project no.
2,40 kW
Nominal power
10,9 A
Nominal current
200-230 V / 50 Hz Nominal voltage range +/-10%
at the indicated power frequency
1 N ~
Current type
Max. operating pressure 15 bar
Max operating pressure in the refrigerating system
(15 bar / 218 PSI)
R 134A - 0,575 kg
Refrigerant type and filling weight
Contains fluorinated greenhouse gases covered by the Kyoto Protocol
Symbol on the type plate
Information
CE conformity marking
Electrical and electronic equipment manufactured / placed on the mar-
ket in the EC after 13 August 2005 and be disposed of in separate col-
lection according to Directive 2012/19/EU on waste electrical and elec-
tronic equipment (WEEE).
GS mark of conformity of the “Deutsche Gesetzliche Unfallversicher-
ung e.V. (DGUV), Prüf- und Zertifizierungsstelle Nahrungsmittel und
Verpackung im DGUV Test“ (German Social Accident Insurance
(DGUV), Testing and Certification Body for Foodstuffs and Packaging
Industry in DGUV Test).
The chamber is certified according to Customs Union Technical Regu-
lation (CU TR) for Russia, Belarus and Kazakhstan.
Nominal temp.
70 °C
2,40 kW / 10,9 A
Max. operating pressure 15 bar
158 °F
200-230 V / 50 Hz
R 134A – 0,575 kg
IP protection
20
Contains fluorinated
Safety device
DIN 12880
1 N ~
greenhouse gases covered
Class
3.1
by the Kyoto Protocol
Art. No.
9020-0328
Project No.
Built
2017
Constant climate chamber
BINDER GmbH
Im Mittleren Ösch 5
78532 Tuttlingen / Germany
www.binder-world.com
KBF P 240
E6
Serial No. 00000000000000
Made in Germany

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1.5 General safety instructions on installing and operating the chambers
With regard to operating the chambers and to the installation location, please observe the DGUV guide-
lines 213-850 on safe working in laboratories (formerly BGI/GUV-I 850-0, BGR/GUV-R 120 or ZH 1/119,
issued by the employers’ liability insurance association) (for Germany.
BINDER GmbH is only responsible for the safety features of the chamber provided skilled electricians or
qualified personnel authorized by BINDER perform all maintenance and repair, and if components relating
to chamber safety are replaced in the event of failure with original spare parts.
To operate the chamber, use only original BINDER accessories or accessories from third-party suppliers
authorized by BINDER. The user is responsible for any risk caused by using unauthorized accessories.
CAUTION
Danger of overheating.
Damage to the chamber.
∅Do NOT install the chamber in unventilated recesses.
Ensure sufficient ventilation for dispersal of the heat.
Do not operate the chambers in hazardous locations.
DANGER
Explosion hazard.
Danger of death.
∅Do NOT operate the chamber in potentially explosive areas.
∅KEEP explosive dust or air-solvent mixtures AWAY from the chamber.
The chambers do not dispose of any measures of explosion protection.
DANGER
Explosion hazard.
Danger of death.
∅Do NOT introduce any substance into the chamber which is combustible or explosive at
working temperature.
∅NO explosive dust or air-solvent mixture in the inner chamber.
Any solvent contained in the charging material must not be explosive or inflammable. I.e., irrespective of
the solvent concentration in the steam room, NO explosive mixture with air must form. The temperature
inside the chamber must lie below the flash point or below the sublimation point of the charging material.
Familiarize yourself with the physical and chemical properties of the charging material, as well as the con-
tained moisture constituent and its behavior with the addition of heat energy and humidity.
Familiarize yourself with any potential health risks caused by the charging material, the contained moisture
constituent or by reaction products that may arise during the temperature process. Take adequate
measures to exclude such risks prior to putting the chamber into operation.

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DANGER
Electrical hazard.
Danger of death.
∅The chamber must NOT become wet during operation or maintenance.
The chambers were produced in accordance with VDE regulations and were routinely tested in accord-
ance to VDE 0411-1 (IEC 61010-1).
During and shortly after operation, the temperature of the inner surfaces almost equals the set-point.
CAUTION
The glass doors, the glass door handles, the inner chamber, and the light cassettes
will become hot during operation.
Danger of burning.
∅Do NOT touch the glass doors, the inner surfaces, the light cassettes or the charging
material during operation.
WARNING
Stability hazard.
Danger of injury.
Damage to the chamber and the charging material.
Housing cover breakaway.
∅Do NOT climb on the lower housing cover.
∅Do NOT load the lower housing cover with heavy objects while the chamber door is
open.

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1.6 Intended use
Constant climate chambers series KBF P / KBF LQC and growth chambers with light and humidity series
KBWF are suitable for exact conditioning of harmless materials. A mixture of any component of the charg-
ing material with air must NOT be explosive. The operating temperature must lie below the flash point or
below the sublimation point of the charging material. Any component of the charging material must NOT
be able to release toxic gases.
Other applications are not approved.
The chambers are not classified as medical devices as defined by the Medical Device Directive
93/42/EEC.
Following the instructions in this operating manual and conducting regular maintenance work
(chap. 22) are part of the intended use.
DANGER
Explosion or implosion hazard.
Danger of poisoning.
Danger of death.
∅Do NOT introduce any substance combustible or explosive at working temperature into
the chamber, in particular no energy sources such as batteries or lithium-ion batteries.
∅NO explosive dust or air-solvent mixture in the inner chamber.
∅Do NOT introduce any substance which could lead to release of toxic gases.
The charging material shall not contain any corrosive ingredients that may damage the ma-
chine components made of stainless steel, aluminum, and copper. Such ingredients include in
particular acids and halides. Any corrosive damage caused by such ingredients is excluded
from liability by BINDER GmbH.
WARNING: If customer should use a BINDER chamber running in non-supervised continuous
operation, we strongly recommend in case of inclusion of irrecoverable specimen or samples
to split such specimen or samples and store them in at least two chambers, if this is feasible.
In case of foreseeable use of the chamber there is no risk for the user through the integration of the
chamber into systems or by special environmental or operating conditions in the sense of EN 61010-
1:2010. For this, the intended use of the chamber and all its connections must be observed.
1.7 Operating instructions
Depending on the application and location of the chamber, the operator of the chamber must provide the
relevant information for safe operation of the chamber in a set of operating instructions.
Keep these operating instructions with the chamber at all times in a place where they are
clearly visible. They must be comprehensible and written in the language of the employees.

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1.8 Measures to prevent accidents
The operator of the chamber must observe the following rule: “Betreiben von Arbeitsmitteln. Betreiben von
Kälteanlagen, Wärmepumpen und Kühleinrichtungen“ (Operation of work equipment. Operation of refrig-
eration systems, heat pumps and refrigeration equipment) (GUV-R 500 chap. 2.35) (for Germany).
The manufacturer took the following measures to prevent ignition and explosions:
•Indications on the type plate
See operating manual chap. 1.4.
•Operating manual
An operating manual is available for each chamber.
•Overtemperature monitoring
The chamber is equipped with a temperature display, which can be read from outside.
The chamber is equipped with an additional safety controller (temperature safety device class 3.1 acc.
to DIN 12880:2007). Visual and audible (buzzer) signals indicate temperature exceeding.
•Safety, measurement, and control equipment
The safety, measuring, and control equipment is easily accessible.
•Electrostatic charge
The interior parts are grounded.
•Non-ionizing radiation
Non-ionizing radiation is not intentionally produced, but released only for technical reasons by electrical
equipment (e.g. electric motors, power cables, solenoids). The machine has no permanent magnets. If
persons with active implants (e.g. pacemakers, defibrillators) keep a safe distance (distance of field
source to implant) of 30 cm, an influence of these implants can be excluded with high probability.
•Protection against touchable surfaces
Tested according to EN ISO 13732-1:2008.
•Floors
See operating manual chap. 3.4 for correct installation
•Cleaning
See operating manual chap. 22.4.
•Examinations
The chamber has been inspected by the “Deutsche Gesetzliche Unfallversicherung e.V. (DGUV)
(German Social Accident Insurance (DGUV)” (German Social Accident Insurance (DGUV), Testing
and Certification Body for Foodstuffs and Packaging Industry in DGUV Test) and bears the GS mark.

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1.9 Resistance of the humidity sensor against harmful substances
The following list of harmful substances refers only to the humidity sensor and does not include any other
materials incorporated in the chamber or prohibited substances in relation to explosion protection.
Some gases - especially clean gases - do not have any influence on the humidity sensor. Others do have
a very small influence, whereas others may influence the sensor to a larger extent.
•The following gases do not influence the sensor and the humidity measurement: Argon (Ar), carbon
dioxide (CO2),helium (He), hydrogen (H2), neon (Ne), nitrogen (N2), nitrous oxide (N2O), oxygen (O2)
•The following gases do not or to a minor extent influence the sensor and the humidity measurement:
Butane (C4H10), ethane (C2H6), methane (CH4), natural gas propane (C3H8)
•The following gases do not, or to a minor extent influence the sensor and the humidity measurement,
provided that the indicated loads are not exceeded:
Maximum work place
threshold limit value
Tolerated concentration
with permanent load
Substance
Formula
ppm
mg/m3
ppm
mg/m3
Ammonia
NH3
20
14
5500
4000
Acetone
CH3COCH3
500
1200
3300
8000
Benzene
300
1200
150000
Chlorine
Cl2
0.5
1.5
0.7
2
Acetic acid
CH3COOH
10
25
800
2000
Ethyl acetate
CH3COOC2H5
400
1400
4000
15000
Ethanol
C2H5OH
500
960
3500
6000
Ethylene glycol
HOCH2CH2OH
10
26
1200
3000
Formaldehyde
HCHO
0.3
0.37
2400
3000
Isopropanol
(CH3)2CHOH
200
500
4800
12000
Methanol
CH3OH
200
260
3500
6000
Methyl ethyl ketone
C2H5COCH3
200
590
3300
8000
Ozone
O3
0.1
0.2
0.5
1
Hydrochloric acid
HCl
2
3
300
500
Hydrogen sulphide
H2S
10
15
350
500
Nitrogen oxides
NOx
5
9
5
9
Sulphur dioxide
SO2
5
13
5
13
Toluol
C6H5CH3
100
380
1300
5000
Xylene
C6H4(CH3)2
100
440
1300
5000
These values are to be considered as approximate values. The sensor resistance largely depends on
the temperature and humidity conditions during the time of exposure to harmful substances. Avoid
simultaneous condensation. Tolerated error of measurement: ± 2 %r.H. The maximum work place
threshold limit value is one that can be regarded as harmless for humans.
•Vapors of oil and fat are dangerous for the sensor because they may condensate at the sensor and
thus prevent its function (insulating layer). For similar reasons it is not possible to measure smoke
gases.

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2. Chamber description and overview
2.1 Chamber description
2.1.1 KBF P / KBF LQC
KBF P und KBF LQC constant climate chambers are equipped with a multifunctional microprocessor dis-
play controller with 2-channel technology for temperature and humidity plus a digital display accurate to
one-tenth of a degree resp. 0.1% r.H. With its comprehensive program control functions, the MB2 display
program controller permits the high precision performance of temperature and humidity cycles. With its
microprocessor controlled humidifying and dehumidifying system the chambers are high-precision con-
stant climate chambers.
The chambers completely meet the requirements for climatic chambers of the stipulated stability and du-
rability tests for pharmaceutical products:
•Stability tests acc. to ICH guideline CPMP/ICH/2736/99 (Q1A)
•Photostability tests acc. to ICH guideline CPMP/ICH/279/95 (Q1B) (KBF P)
Furthermore, thy permit simulating exactly and over long periods constant conditions for other applications
such as sample conditioning for material testing of paper, textiles, plastics, building materials, etc.
The APT.line™ preheating chamber system guarantees high level of spatial and time-based temperature
precision, thanks to the direct and distributed air circulation into the interior. The fan supports exact at-
tainment and maintenance of the desired temperature accuracy.
KBF LQC: The function Light Quantum Control permits integration of UV intensity and luminous intensity
inside the usable volume. The optical sensors used according to the ICH guideline for stability and durabil-
ity tests of pharmaceutical products Q1B. Sensor measurement is to a great extent directionally inde-
pendent, diffused light is also weighted. The function Light Quantum Control permits apart from displaying
the actual values of UVA and the visible spectral range cumulative measurement of the light doses In
Manual Mode target dose values of UVA and the visible spectral range can be entered. When they are
reached, the UVA and cool white fluorescent tubes are automatically turned off and notifying and alarm
messages are released.
2.1.2 KBWF
KBWF growth chambers allow simulating the parameters for natural conditions, such as temperature,
humidity and light. They can be universally used for conditioning of various types of charging material,
even for long-term applications.
The chambers are equipped with a multifunctional microprocessor color display program controller for
temperature, humidity, and illumination control. Temperature is displayed accurate to one-tenth of a de-
gree and humidity to 0.1% r.H. With its microprocessor controlled humidifying and dehumidifying system
the KBWF is a high-precision growth chamber for programmable light and climate conditions. With its
comprehensive program control functions, the MB2 display program controller permits the high precision
performance of temperature and humidity cycles and illumination control. Any climatic conditions can ex-
actly be simulated constantly and precisely over long periods of time.
The chamber is regularly equipped with daylight illumination tubes. It can be optionally equipped with plant
growth lamps bearing the ideal spectrum for photosynthesis and thus become the ideal growing chamber
for plants under programmable climate conditions. Operative ranges are plant biotechnology, agricultural
industry, forestry and timber industry, pharmaceutical and chemical industry, basic research, quality as-
surance and material testing. The fluorescent tubes are built in light cassettes that can be freely posi-
tioned within wide areas. They illuminate very homogeneously the racks below them. The lamps can be
switched in three groups. By adequately adjusting the program controller, an automatic day/night simula-
tion can be effected.

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The horizontal air conduction of the APT.line™ preheating chamber technology, in conjunction with the
controllable air turbine enables simulation of the natural air-flow conditions. The APT.line™ preheating
chamber system guarantees high level of spatial and time-based temperature precision, thanks to the
direct and distributed air circulation into the interior. The fan supports exact attainment and maintenance
of the desired temperature accuracy.
2.1.3 General
A resistance humidifying system humidifies the air. For this purpose, use deionized (demineralized) water.
The option BINDER Pure Aqua Service allows using the chamber with any degree of water hardness.
The inner chamber, the pre-heating chamber and the interior side of the doors are all made of stainless
steel V2A (German material no. 1.4301, US equivalent AISI 304). The housing is RAL 7035 powder-
coated. All corners and edges are also completely coated.
All chamber functions are easy and comfortable to use thanks to their clear arrangement. Major features
are easy cleaning of all chamber parts and avoidance of undesired contamination.
The efficient program controller is equipped with a multitude of operating functions, in addition to recorder
and alarm functions. Programming of test cycles is easily accomplished via the modern MB2 touch screen
controller and is also possible directly with a computer via Intranet in connection with the communication
software APT-COM™ 3 DataControlSystem (option, chap. 21.1). The chamber comes equipped with an
Ethernet serial interface for computer communication. In addition, the BINDER communication software
APT-COM™ 3 permits networking up to 30 chambers and connecting them to a PC for controlling and
programming, as well as recording and representing temperature and humidity data. For further options,
see chap.25.5.
The chambers are equipped with four castors. Both front castors can be easily locked via the attached
brakes.
Temperature range: 0 °C / 32 °F up to 70 °C / 158 °F without light cassettes, 10 °C / 50 °F up to 60 °C /
140 °F with illumination
KBF LQC: When at least one light sensor is plugged-in, the maximum temperature is automatically limited
to 60 °C.
Humidity range: 10% r.H. to 80% r.H.
For the control ranges of temperature and humidity, see climatic diagrams (chap. 18).

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2.2 Chamber overview
Figure 4: Constant climate chamber / Growth chamber size 240
(A) Instrument box
(B) Door handle
(C) Outer door
(D) Refrigerating machine and humidity generation module
2.3 Instrument panel
5,7" controller display with touchscreen
USB interface
Pilot lamp
Figure 5: Instrument panel with MB2 program controller and USB interface
(A)
(B)
(C)
(D)

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2.4 Lateral control panels
(1)
(2)
(3)
(4)
(5)
(6)
(7)
(8)
(9)
right side left side (optional)
Figure 6: Lateral control panels at the sides of the refrigerating / humidity generation module
with optional equipment
(1) Main power switch
(2) DIN socket for additional Pt 100 sensor (may be available via BINDER INDIVIDUAL customized
solutions)
(3) DIN socket for analog outputs (option)
(4) RS485 interface
(5) DIN socket for switching contacts (may be available via BINDER INDIVIDUAL customized solu-
tions)
(6) DIN socket for zero-voltage relay alarm output (option)
(7) Ethernet interface
(8) Temperature safety device class 3.1 (part of option “Safety device class 3.3”)
(9) Temperature safety device class 3.2 (part of option “Safety device class 3.3”)