Binder KBW 240 User manual

Issue 11/2016 Art. N0. 7001-0338
Operating Manual
Translation of the original operating manual
KBW (E6)
Growth chambers with light
with program control and adjustable light cassettes
Model Model version Art. No.
KBW 240 KBW240-230V 9020-0338, 9120-0338
KBW 400 KBW400-230V 9020-0339, 9120-0339
KBW 720 KBW720-230V 9020-0340, 9120-0340
BINDER GmbH
Address: Post office box 102, 78502 Tuttlingen, Germany Phone: [phone]
Fax: [phone] Internet: http://www.binder-world.com
E-mail: [e-mail] Service Hotline: [phone]
Service Fax: [phone] Service E-Mail: [e-mail]
Service Hotline USA: [phone] or [phone] x3
Service Hotline Asia Pacific: [phone] or [phone]
Service Hotline Russia and CIS: [phone]

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Content
1. SAFETY..................................................................................................................6
1.1 Legal considerations ...........................................................................................................................6
1.2 Structure of the safety instructions......................................................................................................6
1.2.1 Signal word panel......................................................................................................................6
1.2.2 Safety alert symbol ....................................................................................................................7
1.2.3 Pictograms.................................................................................................................................7
1.2.4 Word message panel structure .................................................................................................8
1.3 Localization / position of safety labels on the chamber.......................................................................8
1.4 Type plate............................................................................................................................................9
1.5 General safety instructions on installing and operating the chambers..............................................10
1.6 Intended use......................................................................................................................................12
1.7 Operating instructions .......................................................................................................................12
1.8 Measures to prevent accidents .........................................................................................................13
2. CHAMBER DESCRIPTION AND OVERVIEW .....................................................14
2.1 Chamber description.........................................................................................................................14
2.2 Chamber overview ............................................................................................................................15
2.3 Instrument panel................................................................................................................................15
2.4 Lateral control panels........................................................................................................................16
3. COMPLETENESS OF DELIVERY, TRANSPORTATION, STORAGE, AND
INSTALLATION....................................................................................................17
3.1 Unpacking, and checking equipment and completeness of delivery.................................................17
3.2 Guidelines for safe lifting and transportation.....................................................................................18
3.3 Storage..............................................................................................................................................18
3.4 Location of installation and ambient conditions.................................................................................18
4. INSTALLATION AND CONNECTIONS................................................................20
4.1 Spacer for wall distance....................................................................................................................20
4.2 Mounting the flexible tilt protection kit (KBW 400).............................................................................21
4.3 Installation and connection of the light cassettes..............................................................................22
4.4 Electrical connection .........................................................................................................................23
5. FUNCTIONAL OVERVIEW OF THE MB2 CHAMBER CONTROLLER...............24
5.1 Operating functions in normal display...............................................................................................25
5.2 Display views: Normal display, program display, chart-recorder display...........................................26
5.3 Controller icons overview..................................................................................................................27
5.4 Operating modes...............................................................................................................................29
5.5 Controller menu structure..................................................................................................................30
5.5.1 Main menu...............................................................................................................................31
5.5.2 “Settings” submenu .................................................................................................................32
5.5.3 “Service” submenu ..................................................................................................................32
5.6 Principle of controller entries.............................................................................................................33
5.7 Performance during and after power failures....................................................................................33
5.8 Performance when opening the door................................................................................................34
6. START UP............................................................................................................34
6.1 Turning on the chamber....................................................................................................................34
6.2 Controller settings upon start up .......................................................................................................35
7. SET-POINT ENTRY IN “FIXED VALUE” OPERATING MODE...........................36
7.1 Set-point entry through the “Setpoints” menu ...................................................................................37
7.2 Direct setpoint entry via Normal display............................................................................................38
7.3 Automatic correction of the actual value when turning on or off the illumination ..............................38
7.4 Light commutation and special controller functions via operation lines ............................................39

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8. TIMER PROGRAM: STOPWATCH FUNCTION...................................................40
8.1 Starting a timer program ...................................................................................................................40
8.1.1 Performance during program delay time.................................................................................41
8.2 Stopping a running timer program.....................................................................................................41
8.2.1 Pausing a running timer program ............................................................................................41
8.2.2 Cancelling a running timer program ........................................................................................42
8.3 Performance after the end of the program........................................................................................42
9. TIME PROGRAMS ...............................................................................................43
9.1 Starting an existing time program .....................................................................................................43
9.1.1 Performance during program delay time.................................................................................44
9.2 Stopping a running time program......................................................................................................45
9.2.1 Pausing a running time program .............................................................................................45
9.2.2 Cancelling a running time program..........................................................................................45
9.3 Performance after the end of the program........................................................................................45
9.4 Creating a new time program............................................................................................................46
9.5 Program editor: program management.............................................................................................46
9.6 Section editor: Section management ................................................................................................48
9.6.1 Add a new section ...................................................................................................................49
9.6.2 Copy and insert or replace a section.......................................................................................49
9.6.3 Deleting a section....................................................................................................................51
9.7 Value entry for a program section.....................................................................................................51
9.7.1 Section duration.......................................................................................................................52
9.7.2 Set-point ramp and set-point step ...........................................................................................52
9.7.3 Light commutation and special controller functions via operation lines...................................54
9.7.4 Temperature setpoint ..............................................................................................................55
9.7.5 Fan speed setpoint..................................................................................................................55
9.7.6 Tolerance ranges.....................................................................................................................56
9.7.7 Repeating one or several sections within a time program.......................................................57
9.7.8 Saving the time program .........................................................................................................58
10. WEEK PROGRAMS.............................................................................................59
10.1 Starting an existing week program....................................................................................................59
10.2 Cancelling a running week program..................................................................................................60
10.3 Creating a new week program ..........................................................................................................61
10.4 Program editor: program management.............................................................................................62
10.5 Section editor: Section management ................................................................................................63
10.5.1 Add a new section ...................................................................................................................64
10.5.2 Copy and insert or replace a section.......................................................................................65
10.6 Value entry for a program section.....................................................................................................65
10.6.1 Set-point ramp and set-point step modes................................................................................65
10.6.2 Weekday..................................................................................................................................66
10.6.3 Start time .................................................................................................................................66
10.6.4 Temperature setpoint ..............................................................................................................67
10.6.5 Fan speed setpoint..................................................................................................................67
10.6.6 Light commutation and special controller functions via operation lines...................................68
10.7 Deleting a week program ..................................................................................................................68
11. NOTIFICATION AND ALARM FUNCTIONS ........................................................69
11.1 Notification and alarm messages overview.......................................................................................69
11.1.1 Notifications.............................................................................................................................69
11.1.2 Alarm messages......................................................................................................................70
11.2 State of alarm....................................................................................................................................70
11.3 Resetting an alarm, list of active alarms ...........................................................................................71
11.4 Tolerance range settings...................................................................................................................71
11.5 Activating / deactivating the audible alarm (alarm buzzer)................................................................72

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12. TEMPERATURE SAFETY DEVICES...................................................................73
12.1 Over temperature protective device (class 1) ...................................................................................73
12.2 Overtemperature safety controller class 3.1 .....................................................................................73
12.2.1 Safety controller modes...........................................................................................................73
12.2.2 Setting the safety controller.....................................................................................................74
12.2.3 Message and measures in the state of alarm .........................................................................75
12.2.4 Function check ........................................................................................................................75
12.3 Temperature safety device class 3.3 (option) ...................................................................................76
12.3.1 Temperature safety device class 3.1.......................................................................................77
12.3.2 Temperature safety device class 3.2.......................................................................................78
13. USER MANAGEMENT.........................................................................................79
13.1 Authorization levels and password protection...................................................................................79
13.2 Log in.................................................................................................................................................82
13.3 Log out ..............................................................................................................................................83
13.4 User change......................................................................................................................................83
13.5 Password assignment and password change...................................................................................84
13.5.1 Password change....................................................................................................................84
13.5.2 Deleting the password for an individual authorization level.....................................................87
13.5.3 New password assignment for “service” or “admin” authorization level when the password
function was deactivated.........................................................................................................88
13.6 Activation code..................................................................................................................................89
14. GENERAL CONTROLLER SETTINGS................................................................90
14.1 Selecting the controller’s menu language .........................................................................................90
14.2 Selecting the temperature unit ..........................................................................................................90
14.3 Setting date and time ........................................................................................................................91
14.4 Display configuration.........................................................................................................................93
14.4.1 Adapting the display parameters.............................................................................................93
14.4.2 Touchscreen calibration ..........................................................................................................95
14.5 Network and communication.............................................................................................................96
14.5.1 Serial interfaces.......................................................................................................................96
14.5.2 Ethernet...................................................................................................................................97
14.5.3 Web server..............................................................................................................................98
14.5.4 E-Mail.....................................................................................................................................100
14.6 USB menu.......................................................................................................................................102
15. GENERAL INFORMATION ................................................................................103
15.1 Service contact page.......................................................................................................................103
15.2 Current operating parameters.........................................................................................................103
15.3 Event list..........................................................................................................................................104
15.4 Technical chamber information.......................................................................................................105
16. CHART RECORDER DISPLAY..........................................................................106
16.1 Views...............................................................................................................................................106
16.2 Setting the parameters....................................................................................................................108
17. DEFROSTING AT REFRIGERATING OPERATION..........................................110
18. ILLUMINATION SYSTEM...................................................................................111
18.1 Illumination for optimal plant growth................................................................................................111
18.2 Adjustable light cassettes................................................................................................................111
18.3 Illumination control ..........................................................................................................................113
19. OPTIONS............................................................................................................114
19.1 Communication software APT-COM™ 3 DataControlSystem (option)...........................................114
19.2 RS485 interface (option) .................................................................................................................114

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19.3 Data logger kit (option)....................................................................................................................114
19.4 Analog outputs for temperature (option) .........................................................................................115
19.5 Zero-voltage relay alarm outputs for temperature (option)..............................................................115
19.6 Water protected internal socket (option).........................................................................................116
19.7 Additional flexible Pt 100 temperature sensor (available via BINDER INDIVIDUAL customized
solutions).........................................................................................................................................117
19.8 Object temperature display with flexible Pt 100 temperature sensor (option).................................117
20. MAINTENANCE, CLEANING, AND SERVICE...................................................118
20.1 Maintenance intervals, service........................................................................................................118
20.2 Replacement of the fluorescent tubes.............................................................................................119
20.3 Cleaning and decontamination........................................................................................................119
20.3.1 Cleaning.................................................................................................................................120
20.3.2 Decontamination....................................................................................................................121
20.4 Sending the chamber back to BINDER GmbH ...............................................................................122
21. DISPOSAL..........................................................................................................123
21.1 Disposal of the transport packing....................................................................................................123
21.2 Decommissioning............................................................................................................................123
21.3 Disposal of the chamber in the Federal Republic of Germany........................................................123
21.4 Disposal of the chamber in the member states of the EU except for the Federal Republic of
Germany..........................................................................................................................................125
21.5 Disposal of the chamber in non-member states of the EU .............................................................126
22. TROUBLESHOOTING .......................................................................................127
23. TECHNICAL DESCRIPTION..............................................................................129
23.1 Factory calibration and adjustment .................................................................................................129
23.2 Over current protection....................................................................................................................129
23.3 Definition of usable volume.............................................................................................................129
23.4 Technical Data ................................................................................................................................130
23.5 Equipment and options (extract) .....................................................................................................132
23.6 Spare parts and accessories (extract) ............................................................................................133
23.7 Dimensions KBW 240.....................................................................................................................134
23.8 Dimensions KBW 400.....................................................................................................................135
23.9 Dimensions KBW 720.....................................................................................................................136
24. CERTIFICATES AND DECLARATIONS OF CONFORMITY .............................137
24.1 EU Declaration of Conformity..........................................................................................................137
24.2 Certificate for the GS mark of conformity of the “Deutsche Gesetzliche Unfallversicherung e.V.“
(German Social Accident Insurance) DGUV...................................................................................139
25. PRODUCT REGISTRATION ..............................................................................141
26. CONTAMINATION CLEARANCE CERTIFICATE..............................................142
26.1 For chambers located outside USA and Canada............................................................................142
26.2 For chambers located in USA and Canada.....................................................................................145

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Dear customer,
For the correct operation of the chambers, it is important that you read this operating manual completely
and carefully and observe all instructions as indicated. Failure to read, understand and follow the instruc-
tions may result in personal injury. It can also lead to damage to the chamber and/or poor equipment per-
formance.
1. Safety
This operating manual is part of the components of delivery. Always keep it handy for reference. The de-
vice should only be operated by laboratory personnel especially trained for this purpose and familiar with
all precautionary measures required for working in a laboratory. Observe the national regulations on mini-
mum age of laboratory personnel. To avoid injuries and damage observe the safety instructions of the
operating manual.
WARNING
Failure to observe the safety instructions.
Serious injuries and chamber damage.
Observe the safety instructions in this operating manual.
Carefully read the complete operating instructions of the chambers.
1.1 Legal considerations
This operating manual is for informational purposes only. It contains information for installing, start-up,
operation and maintenance of the product. Note: the contents and the product described are subject to
change without notice.
Understanding and observing the instructions in this operating manual are prerequisites for hazard-free
use and safety during operation and maintenance. In no event shall BINDER be held liable for any dam-
ages, direct or incidental arising out of or related to the use of this manual.
This operating manual cannot cover all conceivable applications. If you would like additional information,
or if special problems arise that are not sufficiently addressed in this manual, please ask your dealer or
contact us directly by phone at the number located on page one of this manual
Furthermore, we emphasize that the contents of this operating manual are not part of an earlier or existing
agreement, description, or legal relationship, nor do they modify such a relationship. All obligations on the
part of BINDER derive from the respective purchase contract, which also contains the entire and exclu-
sively valid statement of warranty administration. The statements in this manual neither augment nor re-
strict the contractual warranty provisions.
1.2 Structure of the safety instructions
In this operating manual, the following safety definitions and symbols indicate dangerous situations follow-
ing the harmonization of ISO 3864-2 and ANSI Z535.6.
1.2.1 Signal word panel
Depending on the probability of serious consequences, potential dangers are identified with a signal word,
the corresponding safety color, and if appropriate, the safety alert symbol.
DANGER
Indicates an imminently hazardous situation that, if not avoided, will result in death or serious
(irreversible) injury.

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WARNING
Indicates a potentially hazardous situation which, if not avoided, could result in death or serious
(irreversible) injury.
CAUTION
Indicates a potentially hazardous situation which, if not avoided, may result in moderate or minor
(reversible) injury.
CAUTION
Indicates a potentially hazardous situation which, if not avoided, may result in damage to the product
and/or its functions or of a property in its proximity.
1.2.2 Safety alert symbol
Use of the safety alert symbol indicates a risk of injury.
Observe all measures that are marked with the safety alert symbol in order to avoid death or
injury.
1.2.3 Pictograms
Warning signs
Electrical hazard
Hot surface
Explosive atmosphere Stability hazard
Lifting hazard Risk of corrosion and /
or chemical burns
Harmful substances
Biohazard
Pollution Hazard
Mandatory action signs
Mandatory regulation
Read operating
instructions
Disconnect the power
plug
Lift with mechanical
assistance
Environment protection
Wear protective gloves
Wear safety goggles

KBW (E6) 11/2016 page 8/147
Prohibition signs
Do NOT touch Do NOT spray with
water
Do NOT climb
Information to be observed in order to ensure optimum function of the product.
1.2.4 Word message panel structure
Type / cause of hazard.
Possible consequences.
∅Instruction how to avoid the hazard: prohibition
Instruction how to avoid the hazard: mandatory action.
Observe all other notes and information not necessarily emphasized in the same way, in order to avoid
disruptions that could result in direct or indirect injury or property damage.
1.3 Localization / position of safety labels on the chamber
The following labels are located on the chamber:
Pictograms (warning signs)
Hot surface (inner glass door above the glass door handle)
Service label
Keep safety labels complete and legible.
Replace safety labels that are no longer legible. Contact BINDER Service for these replacements.

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1.4 Type plate
The type plate sticks to the left side of the chamber, bottom right-hand.
Figure 1: Type plate (example KBW 240 regular chamber)
Indications of the type plate (example)
Information
BINDER
Manufacturer: BINDER GmbH
KBW 240
Model designation
Growth chamber
Device name
Built
2017
Serial no. of the chamber
Serial No.
00000000000000
Year of construction
Nominal temperature
70 °C / 158 °F
Nominal temperature
IP protection
20
IP type of protection acc. to standard EN 60529
Temp. safety device DIN 12880 Temperature safety device acc. to standard DIN
12880:2007
Class
3.1
Class of temperature safety device
Art. No.
9020-0338
Art. no. of the chamber
Project No.
---
Optional: Special application acc. to project no.
1,40 kW
Nominal power
6,1 A
Nominal current
200-230 V / 50 Hz Nominal voltage range +/-10%
at the indicated power frequency
1 N ~
Current type
Max. operating pressure 15 bar
Max operating pressure in the refrigerating system
(15 bar / 218 PSI)
R 134A - 0,27 kg
Refrigerant type and filling weight
Contains fluorinated greenhouse gases covered by the Kyoto Protocol
Symbol on the type plate
Information
CE conformity marking
Electrical and electronic equipment manufactured / placed on the mar-
ket in the EC after 13 August 2005 and be disposed of in separate col-
lection according to Directive 2012/19/EU on waste electrical and elec-
tronic equipment (WEEE).
GS mark of conformity of the “Deutsche Gesetzliche Unfallversicher-
ung e.V. (DGUV), Prüf- und Zertifizierungsstelle Nahrungsmittel und
Verpackung im DGUV Test“ (German Social Accident Insurance
(DGUV), Testing and Certification Body for Foodstuffs and Packaging
Industry in DGUV Test).
The chamber is certified according to Customs Union Technical Regu-
lation (CU TR) for Russia, Belarus and Kazakhstan.
Nominal temp.
70 °C
1,40 kW / 6,1 A
Max. operating pressure 15 bar
158 °F
200-230 V / 50 Hz
R 134A – 0,27 kg
IP protection
20
Contains fluorinated
Safety device
DIN 12880
1 N ~
greenhouse gases covered
Class
3.1
by the Kyoto Protocol
Art. No.
9020-0338
Project No.
Built
2017
Growth chamber
BINDER GmbH
Im Mittleren Ösch 5
78532 Tuttlingen / Germany
www.binder-world.com
KBW 240
E6
Serial No. 00000000000000
Made in Germany

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1.5 General safety instructions on installing and operating the chambers
With regard to operating the chambers and to the installation location, please observe the DGUV guide-
lines 213-850 on safe working in laboratories (formerly BGI/GUV-I 850-0, BGR/GUV-R 120 or ZH 1/119,
issued by the employers’ liability insurance association) (for Germany.
BINDER GmbH is only responsible for the safety features of the chamber provided skilled electricians or
qualified personnel authorized by BINDER perform all maintenance and repair, and if components relating
to chamber safety are replaced in the event of failure with original spare parts.
To operate the chamber, use only original BINDER accessories or accessories from third-party suppliers
authorized by BINDER. The user is responsible for any risk caused by using unauthorized accessories.
CAUTION
Danger of overheating.
Damage to the chamber.
∅Do NOT install the chamber in unventilated recesses.
Ensure sufficient ventilation for dispersal of the heat.
Do not operate the chambers in hazardous locations.
DANGER
Explosion hazard.
Danger of death.
∅Do NOT operate the chamber in potentially explosive areas.
∅KEEP explosive dust or air-solvent mixtures AWAY from the chamber.
The chambers do not dispose of any measures of explosion protection.
DANGER
Explosion hazard.
Danger of death.
∅Do NOT introduce any substance into the chamber which is combustible or explosive at
working temperature.
∅NO explosive dust or air-solvent mixture in the inner chamber.
Any solvent contained in the charging material must not be explosive or inflammable. I.e., irrespective of
the solvent concentration in the steam room, NO explosive mixture with air must form. The temperature
inside the chamber must lie below the flash point or below the sublimation point of the charging material.
Familiarize yourself with the physical and chemical properties of the charging material, as well as the con-
tained moisture constituent and its behavior with the addition of heat energy.
Familiarize yourself with any potential health risks caused by the charging material, the contained moisture
constituent or by reaction products that may arise during the temperature process. Take adequate
measures to exclude such risks prior to putting the chamber into operation.

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DANGER
Electrical hazard.
Danger of death.
∅The chamber must NOT become wet during operation or maintenance.
The chambers were produced in accordance with VDE regulations and were routinely tested in accord-
ance to VDE 0411-1 (IEC 61010-1).
During and shortly after operation, the temperature of the inner surfaces almost equals the set-point.
CAUTION
The glass doors, the glass door handles, the inner chamber, and the light cassettes
will become hot during operation.
Danger of burning.
∅Do NOT touch the glass doors, the inner surfaces, the light cassettes or the charging
material during operation.
WARNING
Stability hazard.
Danger of injury.
Damage to the chamber and the charging material.
Housing cover breakaway.
∅Do NOT climb on the lower housing cover.
∅Do NOT load the lower housing cover with heavy objects while the chamber door is
open.

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1.6 Intended use
Growth chambers with light KBW are suitable for exact simulation of growth conditions and conditioning of
harmless materials. Because of their precise temperature accuracy these devices are especially useful for
incubation of cultures at a standard temperature of 37 °C. A mixture of any component of the charging
material with air must NOT be explosive. The operating temperature must lie below the flash point or be-
low the sublimation point of the charging material. Any component of the charging material must NOT be
able to release toxic gases.
Other applications are not approved.
The chambers are not classified as medical devices as defined by the Medical Device Directive
93/42/EEC.
Following the instructions in this operating manual and conducting regular maintenance work
(chap. 20) are part of the intended use.
DANGER
Explosion or implosion hazard.
Danger of poisoning.
Danger of death.
∅Do NOT introduce any substance combustible or explosive at working temperature into
the chamber, in particular no energy sources such as batteries or lithium-ion batteries.
∅NO explosive dust or air-solvent mixture in the inner chamber.
∅Do NOT introduce any substance which could lead to release of toxic gases.
The charging material shall not contain any corrosive ingredients that may damage the ma-
chine components made of stainless steel, aluminum, and copper. Such ingredients include in
particular acids and halides. Any corrosive damage caused by such ingredients is excluded
from liability by BINDER GmbH.
WARNING: If customer should use a BINDER chamber running in non-supervised continuous
operation, we strongly recommend in case of inclusion of irrecoverable specimen or samples
to split such specimen or samples and store them in at least two chambers, if this is feasible.
In case of foreseeable use of the chamber there is no risk for the user through the integration of the
chamber into systems or by special environmental or operating conditions in the sense of EN 61010-
1:2010. For this, the intended use of the chamber and all its connections must be observed.
1.7 Operating instructions
Depending on the application and location of the chamber, the operator of the chamber must provide the
relevant information for safe operation of the chamber in a set of operating instructions.
Keep these operating instructions with the chamber at all times in a place where they are
clearly visible. They must be comprehensible and written in the language of the employees.

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1.8 Measures to prevent accidents
The operator of the chamber must observe the following rule: “Betreiben von Arbeitsmitteln. Betreiben von
Kälteanlagen, Wärmepumpen und Kühleinrichtungen“ (Operation of work equipment. Operation of refrig-
eration systems, heat pumps and refrigeration equipment) (GUV-R 500 chap. 2.35) (for Germany).
The manufacturer took the following measures to prevent ignition and explosions:
•Indications on the type plate
See operating manual chap. 1.4.
•Operating manual
An operating manual is available for each chamber.
•Overtemperature monitoring
The chamber is equipped with a temperature display, which can be read from outside.
The chamber is equipped with an additional safety controller (temperature safety device class 3.1 acc.
to DIN 12880:2007). Visual and audible (buzzer) signals indicate temperature exceeding.
•Safety, measurement, and control equipment
The safety, measuring, and control equipment is easily accessible.
•Electrostatic charge
The interior parts are grounded.
•Non-ionizing radiation
Non-ionizing radiation is not intentionally produced, but released only for technical reasons by electrical
equipment (e.g. electric motors, power cables, solenoids). The machine has no permanent magnets. If
persons with active implants (e.g. pacemakers, defibrillators) keep a safe distance (distance of field
source to implant) of 30 cm, an influence of these implants can be excluded with high probability.
•Protection against touchable surfaces
Tested according to EN ISO 13732-1:2008.
•Floors
See operating manual chap. 3.4 for correct installation
•Cleaning
See operating manual chap. 20.3.
•Examinations
The chamber has been inspected by the “Deutsche Gesetzliche Unfallversicherung e.V. (DGUV)
(German Social Accident Insurance (DGUV)” (German Social Accident Insurance (DGUV), Testing
and Certification Body for Foodstuffs and Packaging Industry in DGUV Test) and bears the GS mark.

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2. Chamber description and overview
2.1 Chamber description
A high level of precision, reliability, and safety for all growth parameters ensures optimum incubation con-
ditions. Moreover, the KBW growth chamber with light is designed for maximum usability – even in contin-
uous operation year after year. It fulfills all technical and application-specific requirements arising in exper-
imentation such as in the areas of biotechnology, medicine, the nutrition industry, pharmaceutical and
cosmetics industries, botany, and zoology.
The chambers are equipped with a multifunctional microprocessor color display program controller for
temperature, and illumination control. Temperature is displayed accurate to one-tenth of a degree. The
KBW is a high-precision growth chamber for programmable light and temperature conditions. With its
comprehensive program control functions, the MB2 display program controller permits the high precision
performance of temperature cycles and illumination control.
Two important temperature technologies have been combined to achieve perfect temperature control. The
specially developed DCT™ refrigerating system, a direct refrigerating process, in conjunction with the
APT.line™ preheating chamber technology, satisfies the unique prerequisites for attaining highly-precise
temperature control and particularly short recovery times after opening the door.
The refrigerating system is distinguished by direct, precise, and rapid temperature conduction. Large-area
labyrinth evaporator plates directly conduct the cold to the atmosphere of the working space.
The horizontal air conduction of the APT.line™ preheating chamber technology, in conjunction with the
controllable air turbine enables simulation of the natural air-flow conditions. The APT.line™ preheating
chamber system guarantees high level of spatial and time-based temperature precision, thanks to the
direct and distributed air circulation into the interior. This is especially important for maintaining tempera-
tures – especially with full chambers – and for rapid restoration of optimum growth conditions after open-
ing the door. The inner glass door ensures that the temperature remains constant when observing the
incubation process. The fan supports exact attainment and maintenance of the desired temperature accu-
racy. The fan speed is digitally adjustable. The heating and refrigerating systems are microprocessor
regulated to a tenth of a degree. In addition, the growth chamber with light provides almost unlimited pos-
sibilities for adaptation to individual customer requirements based upon extensive programming options of
the controller.
The chamber is regularly equipped with daylight illumination tubes. It can be optionally equipped with plant
growth lamps bearing the ideal spectrum for photosynthesis and thus become the ideal growing chamber
for plants under programmable temperature conditions. The fluorescent tubes are built in light cassettes
that can be freely positioned within wide areas. They illuminate very homogeneously the racks below
them. The lamps can be switched in three groups. By adequately adjusting the program controller, an
automatic day/night simulation can be effected.
All chamber functions are easy and comfortable to use thanks to their clear arrangement. Major features
are easy cleaning of all chamber parts and avoidance of undesired contamination.
The inner chamber, the pre-heating chamber and the interior side of the doors are all made of stainless
steel V2A (German material no. 1.4301, US equivalent AISI 304). The housing is RAL 7035 powder-
coated. All corners and edges are completely coated.
The efficient program controller is equipped with a multitude of operating functions, in addition to recorder
and alarm functions. Programming of test cycles is easily accomplished via the modern MB2 touch screen
controller and is also possible directly with a computer via Intranet in connection with the communication
software APT-COM™ 3 DataControlSystem (option, chap. 19.1). The chamber comes equipped with an
Ethernet serial interface for computer communication. In addition, the BINDER communication software
APT-COM™ 3 permits networking up to 30 chambers and connecting them to a PC for controlling and
programming, as well as recording and representing temperature data. For further options, see chap.23.5.
The chambers are equipped with four castors. Both front castors can be easily locked via the attached
brakes.
Temperature range at an ambient temperature of 22 °C +/- 3 °C / 71.6 °F ± 5.4 °F:
•Without light cassettes: 0 °C / 32 °F up to +70 °C / 158 °F.
•With maximum illumination +10 °C / 41 °F up to +60 °C / 140 °F

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2.2 Chamber overview
Figure 2: Growth chamber with light KBW 240
(A) Instrument box
(B) Door handle
(C) Outer door
(D) Refrigerating machine
2.3 Instrument panel
5,7" controller display with touchscreen
USB interface
Pilot lamp
Figure 3: Instrument panel with MB2 program controller and USB interface
(A)
(B)
(C)
(D)

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2.4 Lateral control panels
(1)
(2)
(3)
(4)
(5)
(6)
(7)
(8)
(9)
right side left side (optional)
Figure 4: Lateral control panels at the sides of the refrigerating module
with optional equipment
(1) Main power switch
(2) DIN socket for additional Pt 100 sensor (may be available via BINDER INDIVIDUAL customized
solutions)
(3) DIN socket for analog outputs (option)
(4) RS485 interface
(5) DIN socket for switching contacts (may be available via BINDER INDIVIDUAL customized solu-
tions)
(6) DIN socket for zero-voltage relay alarm output (option)
(7) Ethernet interface
(8) Temperature safety device class 3.1 (part of option “Safety device class 3.3”)
(9) Temperature safety device class 3.2 (part of option “Safety device class 3.3”)

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3. Completeness of delivery, transportation, storage, and installa-
tion
3.1 Unpacking, and checking equipment and completeness of delivery
After unpacking, please check the chamber and its optional accessories, if any, based on the delivery
receipt for completeness and for transportation damage. Inform the carrier immediately if transportation
damage has occurred.
The final tests of the manufacturer may have caused traces of the shelves on the inner surfaces. This has
no impact on the function and performance of the chamber.
Please remove any transportation protection devices and adhesives in/on the chamber and on the doors
and remove the operating manuals and accessory equipment.
CAUTION
Sliding or tilting of the chamber.
Damage to the chamber.
Risk of injury by lifting heavy loads.
∅Do NOT lift or transport the chamber using the door, the handle, or the lower housing.
∅Do NOT lift the chamber by hand.
Lift the chamber from the pallet using technical devices (fork lifter). Set the fork lifter
only from the front or rear in the middle of the chamber.
∅Do NOT set the fork lifter from the chamber side.
If you need to return the chamber, please use the original packing and observe the guidelines for safe
lifting and transportation (chap. 3.2).
For disposal of the transport packing, see chap. 21.1.
Note on second-hand chambers (Ex-Demo-Units):
Second-hand chambers are chambers that were used for a short time for tests or exhibitions. They are
thoroughly tested before resale. BINDER ensures that the chamber is technically sound and will work
flawlessly.
Second-hand chambers are marked with a sticker on the chamber door. Please remove the sticker before
commissioning the chamber.

KBW (E6) 11/2016 page 18/147
3.2 Guidelines for safe lifting and transportation
The front castors can be blocked by brakes. After operation, please observe the guidelines for temporarily
decommissioning the chamber (chap. 21.2). Please move the chambers with castors only when empty
and on an even surface, otherwise the castors may be damaged.
CAUTION
Sliding or tilting of the chamber.
Damage to the chamber.
Risk of injury by lifting heavy loads.
Transport the chamber in its original packaging only.
For moving or shipping, secure the chamber with transport straps.
∅Do NOT lift or transport the chamber using the door, the handle, or the lower housing.
∅Do NOT lift the chamber by hand.
Lift the chamber using technical devices (fork lifter). Set the fork lifter only from the
front or rear in the middle of the chamber.
∅Do NOT set the fork lifter from the chamber side.
•Permissible ambient temperature range during transport: -10 °C / 14°F to +60 °C / 140°F
You can order transport packing for moving or shipping purposes from BINDER service.
3.3 Storage
Intermediate storage of the chamber is possible in a closed and dry room. Observe the guidelines for
temporary decommissioning (chap. 21.2).
•Permissible ambient temperature range during storage: -10 °C / 14°F to +60 °C / 140°F
•Permissible ambient humidity: max. 70 % r.H., non-condensing
When after storage in a cold location you transfer the chamber to its warmer installation site, condensa-
tion may form. Before start-up, wait at least one hour until the chamber has attained ambient temperature
and is completely dry.
3.4 Location of installation and ambient conditions
Set up the chamber on a flat, even surface, and in a well-ventilated, dry location and align it using a spirit
level. The site of installation must be capable of supporting the chamber’s weight (see technical data,
chap. 23.4). The chambers are designed for setting up inside a building (indoor use).
CAUTION
Danger of overheating.
Damage to the chamber.
∅Do NOT set up chamber in non-ventilated recesses.
Ensure sufficient ventilation for dispersal of the heat.

KBW (E6) 11/2016 page 19/147
•Permissible ambient temperature range during operation: +18 °C / 64.4 °F to +32 °C / 89.6 °F. At ele-
vated ambient temperature values, fluctuations in temperature can occur.
The ambient temperature should not be substantially higher than the indicated ambient tem-
perature of +22 °C +/- 3 °C / 71.6 °F ± 5.4 °F to which the specified technical data relate. Devi-
ations from the indicated data are possible for other ambient conditions. Lower values of the
temperature range indicated in the technical data are valid at an ambient temperature of max.
25 °C / 77 °F.
With each degree of ambient temperature >25 °C / 77 °F, the refrigeration power decreases by
1.5 K.
•Permissible ambient humidity: 70 % r.H. max., non-condensing
When operating the chamber at temperature set-points below ambient temperature, high ambient humidi-
ty may lead to condensation on the chamber.
•Installation height: max. 2000 m / 6562 ft. above sea level.
When placing several chambers of the same size side by side, maintain a minimum distance of 250 mm /
9.84 in between each chamber. Wall distances: rear 100 mm / 3.9 in, sides 160 mm / 6.29 in. Spacing
above the chamber of at least 100 mm / 3.9 inmust also be accounted for.
CAUTION
Danger by stacking.
Damage to the chambers.
∅Do NOT place the chambers on top of each other.
To completely separate the chamber from the power supply, you must disconnect the power plug. Install
the chamber in a way that the power plug is easily accessible and can be easily pulled in case of danger.
For the user there is no risk of temporary overvoltages in the sense of EN 61010-1:2010.
With an increased amount of dust in the ambient air, clean the condenser fan (by suction or blowing) sev-
eral times a year.
Avoid any conductive dust in the ambiance according to the chamber layout complying with pollution de-
gree 2 (IEC 61010-1).
Do not install or operate the chamber in potentially explosive areas.
DANGER
Explosion hazard.
Danger of death.
∅Do NOT operate the chamber in potentially explosive areas.
KEEP explosive dust or air-solvent mixtures AWAY from the vicinity of the chamber.

KBW (E6) 11/2016 page 20/147
4. Installation and connections
4.1 Spacer for wall distance
Please fix both spacers with the supplied screws at the chamber rear. This serves to ensure the pre-
scribed minimum distance to the rear wall of 100 mm / 3.94 in.
Figure 5: Spacer for wall distance
Figure 6: Chamber rear with mounted spacers