Bio-Tek 405 TS User manual

Getting Started Guide
Microplate Washer
405™ TS

Quick Start
Here are guidelines for quickly getting up and running with the 405™
TS Microplate Washer:
Install the 405 TS
Follow instructions for removing shipping
hardware and installing the instrument.
page 9
Optimize performance
Review the best practices for optimal
performance.
page 37
Review the Safety Information page 85
Copy the 405 TS Operator's Manual to
your computer to make it easy to find
reference information in the future. The
complete operator's manual is delivered
on the USB flash drive shipped with the
instrument.
Visit us at www.biotek.com.
1171009
Rev C

2 | 405 TS Getting Started Guide
Contents
Quick Start 1
Notices 4
Global Service and Support 5
BioTek's Customer Resource Center 7
Install the 405 TS 9
Unpack and Inspect the Instrument 10
Remove the Shipping Hardware 10
Setting Up the 405 TS 14
Install Software/Connect to Computer 20
Connect to Power 21
Define Instrument Settings 22
Verify Performance 25
Basic Operation 29
Touch Screen Basics 30
Create or Modify a Protocol 32
Verify Performance 34
Optimize Performance 37
Predefined Protocols Listing 41
Predefined Sample Protocols 42
Operating with the BioStack 47
Use the BioStack 49
Cell Wash 51
Biomagnetic Separation - Magnetic Bead Assays 56
Manifold Stop Screw Adjustment Kit 62
Vacuum Filtration for Filter Plate Assays 64
Changing the Instrument's Settings 68
Washer Settings 69
AutoPrime 70
Maintenance 75
Overview 76
Recommended Maintenance Schedule 77
Daily Maintenance 79
Overnight/Multi-Day Maintenance 79
AutoClean the Washer 80
Removing Protein Residuals and Fungi Growth 82
Safeguards and Troubleshooting 85
Hazards and Precautions 86
CE Mark 89
Electromagnetic Interference and Susceptibility 90
Safety Symbols 92
Troubleshooting 94
405™ TS Microplate Washer

| 3
Washer Problems 95
Specifications and Part Numbers 103
Physical Specifications 104
Performance Specifications 105
Package Contents 107
Optional Accessories 110
113
Chemical Compatibility 116
405 TS Customer Training Guide 117
BioTek Instruments, Inc.
Index ...............................................................................................................

4 | 405 TS Getting Started Guide
Notices
BioTekInstruments, Inc.
Highland Park, P.O. Box 998
Winooski, Vermont 05404-0998 USA
All Rights Reserved
© 2013, BioTek®Instruments, Incorporated. No part of this publication may be
reproduced, transcribed, or transmitted in any form, or by any means electronic
or mechanical, including photocopying and recording, for any purpose other
than the purchaser’s use without written permission of BioTek Instruments, Inc.
Trademarks
BioTek®is a registered trademark and 405™ TS Microplate Washeris a
trademark of BioTek Instruments, Inc.
Microsoft®, Internet Explorer®, Windows®, Windows 7, Windows 8, Windows
Vista®, Windows XP, and Excel®are either registered trademarks or trademarks
of Microsoft Corporation in the United States and/or other countries.
All other trademarks are the property of their respective holders.
Restrictions and Liabilities
Information in this document is subject to change and does not represent a
commitment by BioTek Instruments, Inc. Changes made to the information in
this document will be incorporated in new editions of the publication. No
responsibility is assumed by BioTek for the use or reliability of software or
equipment that is not supplied by BioTek or its affiliated dealers.
405™ TS Microplate Washer

Global Service and Support| 5
Global Service and Support
BioTek instrument service and repair is available worldwide at several of BioTek’s
International Service Centers and in the field at your location. Contact the office
nearest you to arrange service or to get answers to your technical questions, call the
Technical Assistance Center (TAC) at 802-655-4740 in the US.
BioTek World Headquarters US BioTek China
Mailing Address:
PO Box 998, Highland Park
Winsooki, VT 05404-0998 United States
Service Shipping Address:
15 Tigan Street
Winooski, VT 05404 United States
Room 304, Tower D
Ocean International Center
62 Middle 4th East Ring Road
Chaoyang District
Beijing 100024
P.R. China
Phone: [phone]
Fax: [phone]
Phone: [phone]
Fax: [phone]
Email: [e-mail]
Website: www.biotek.com
Email: [e-mail]
Website: www.biotekchina.com.cn
BioTek Germany Service Center
& European Coordination Center
BioTek Instruments SAS
Bureau de liaison France
Kocherwaldstrasse 34 D-74177
Bad Friedrichshall
Germany
50 avenue d’Alsace
68025 Colmar Cedex
France
Phone: [phone]
Fax: [phone]
Phone: [phone]
Fax: [phone]
Email: [e-mail]
Website: www.biotek.de
Email: [e-mail]
Website: www.biotek.fr
BioTek India BioTek Singapore
Unit 223, Linkway Estate
New Link Road, Malad West
Mumbai 400064
India
20 Science Park Road #01-08A
Teletech Park
Singapore 117674
Phone: [phone]
Fax: [phone]
Phone: [phone]
Fax: [phone]
Email: [e-mail]
Website: www.biotek.in
Email: [e-mail]
Website: www.biotek.com
BioTek Instruments, Inc.

6 | 405 TS Getting Started Guide
BioTek South Korea BioTek Switzerland
3F, Gyungnam building, 830-48
Yeoksam-dong, Gangnam-gu
Seoul, South Korea (135-936)
Zentrum FanhÖfli 8
6014 Luzern
Switzerland
Phone: [phone]
Fax: [phone]
Phone: [phone]
Fax: [phone]
Email: [e-mail]
Website: www.biotekinstruments.co.kr
Email: [e-mail]
Website: www.biotek.ch
BioTek United Kingdom (UK)
6 Bull Street
Potton, Bedfordshire SG19 2NR
United Kingdom
Phone: [phone]
Fax: [phone]
Email: [e-mail]
Website: www.biotek.uk.com
405™ TS Microplate Washer

BioTek's Customer Resource Center| 7
BioTek's Customer Resource Center
BioTek's Customer Resource Center (CRC) continues our tradition of superior service
and support. After an easy registration process, you can access lots of useful
information about your BioTek microplate instrumentation and software. On the
secure CRC website, you can:
lTrack orders
lAccess warranty information, user manuals and software updates
lDownload technical and application information
lMaintain equipment inventory (product registration)
lRequest service and technical support
lView service history
lAnd much more!
Register at https://customer.biotek.com
Intended Use Statement
lThe 405™ TS Microplate Washer provides microplate priming, washing, and
dispensing for ELISA™, fluorescence and chemiluminescence immunoassays,
cellular and agglutination assays.
lIf the instrument has an "IVD" label it may be used for clinical and non-clinical
purposes, including research and development. If there is no such label the
instrument may only be used for research and development and non-clinical
purposes.
Warnings
Operate the instrument on a level, stable surface away from excessive
humidity.
When operated in a safe environment, according to the instructions in this
document, there are no known hazards associated with the 405 TS.
However, the operator should be aware of certain situations that could
result in serious injury; these vary depending on the instrument type. See
Hazards and Precautions.
Strict adherence to instrument maintenance and qualification procedures
is required to ensure accurate dispense volumes and risk-free operation.
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8 | 405 TS Getting Started Guide
Quality Control
It is considered good laboratory practice to run laboratory samples according to
instructions and specific recommendations included in the assay package insert for
the test to be conducted. Failure to conduct Quality Control checks could result in
erroneous test data.
Warranty and Product Registration
Please take a moment to review the Warranty information that shipped with your
product. Please also register your product with BioTek to ensure that you receive
important information and updates about the product(s) you have purchased.
You can register online through BioTek’s Customer Resource Center (CRC) at
www.biotek.com or by calling 888/451-5171 or 802/655-4740.
Repackaging and Shipping
If you need to ship the instrument to BioTek for service or repair, contact BioTek
for a Return Materials Authorization (RMA) number and use the original packing
materials. Other forms of commercially available packaging are not recommended
and can void the warranty. If the original packing materials have been damaged or
lost, contact BioTek for replacement packing.
405™ TS Microplate Washer

Install the 405 TS
Follow the instructions in this section to install your
instrument.
Unpack and Inspect the Instrument 10
Remove the Shipping Hardware 10
Setting Up the 405 TS 14
Install Software/Connect to Computer 20
Connect to Power 21
Define Instrument Settings 22
Verify Performance 25

10 | 405 TS Getting Started Guide
Unpack and Inspect the Instrument
Important: Save all packaging materials. If you need to ship the instrument or
accessories to BioTek for repair or replacement, you must use the original packaging.
Using other forms of commercially available packaging is not recommended and can void
the warranty. Improper packaging that results in damage to the instrument may lead to
additional charges. Refer to the operator's manual for repacking instructions.
Inspect the shipping box, packaging, instrument, and accessories for signs of
damage.
If the 405™ TS Microplate Washer is damaged, notify the carrier and your BioTek
representative. Keep the shipping cartons and packing material for the carrier’s
inspection. BioTek will arrange for repair or replacement of your instrument
immediately, before the shipping-related claim is settled.
1. Unpack the boxes containing the instrument and other equipment:
l405™ TS Microplate Washer and accessories
lVacuum Pump and accessories
lVacuum Filtration Accessory Kit
2. Place all packing materials back into the shipping boxes for reuse if necessary.
Refer to the Package Contents on page 107 to make sure you have all expected
equipment.
Remove the Shipping Hardware
The 405 TS is shipped with a protective manifold shipping bracket. Remove this
bracket before using the washer and reinstall it prior to shipping to avoid
irreparable damage to the manifold. Failure to remove and reinstall the shipping
bracket may void your warranty.
Keep in mind that you must reinstall the shipping hardware and use the original
shipping material if it is necessary to return the instrument to BioTek for service or
repair.
405™ TS Microplate Washer

Remove the Shipping Hardware| 11
Remove the manifold shipping bracket
1. Use the 9/64” (3.57 mm) hex wrench to
unscrew the cap screws at the base of
the shipping bracket and remove it.
2. Slide the bracket towards you and
remove.
lMost brackets are black, unlike
the one shown here.
3. Store the bracket: mount it on the back
panel, on the studs provided.
lDirect Drain Waste System: if you are
using the direct drain waste method,
wait till later to mount the shipping
bracket. For direct drain, an
intermediate collection bottle is
attached to the washer using the
same studs as the shipping bracket.
See the Direct Drain Kit instructions.
405 TS with Verify™ Technology "Q" Models
"Q" Models have
three shipping
brackets:
remove two
before powering
up, and the last
one after start
up.
BioTek Instruments, Inc.

12 | 405 TS Getting Started Guide
1. Use the 9/64” hex wrench to unscrew the
cap screws and remove the bracket on the
side of the wash manifold that holds the
Verify sensor in place.
2. Remove the large bracket in front holding
the wash manifold: Remove the three
screws. Slightly lift the manifold(s) to
release the bracket from the base, and pull
it away from the manifold(s).
3. Set the brackets, screws and washers aside.
Follow instructions to set up the washer, e.g.
install the vacuum pump and tubing. Then,
perform Step 4, below.
4. When you're ready to power up the
instrument, plug it in and turn it on.
Release the latch on the left side of the
Verify™ sensor unit, and to lift it up to
remove the third shipping bracket.
5. For safe keeping, put the cap screws and
washers for the two thin brackets in the
studs provided on the longest, last-
removed bracket.
6. Put the manifold bracket's top screw back
in its hole. Save the two longer screws for
storing all the brackets on the back of the
instrument.
405™ TS Microplate Washer

Remove the Shipping Hardware| 13
Layer the brackets on top of each
other on the studs on the back.
7. Store the brackets: mount them on the back
panel on the studs provided. Beginning
with the longest (3rd) bracket, layer the
brackets on top of each other, and use the
longest screws to secure them in place.
BioTek Instruments, Inc.

14 | 405 TS Getting Started Guide
Setting Up the 405 TS
Important: Avoid excessive humidity. Condensation directly on the sensitive
electronic circuits can cause the instrument to fail internal self checks.
Install the instrument on a level, stable surface in an area where ambient
temperatures between 10°C (50°F) and 40°C (104°F) can be maintained.
The instrument should be operated in a non-condensing humid environment
having a maximum relative humidity of 80% at temperatures up to 31°C
decreasing linearly to 50% relative humidity at 40°C.
Connect the Vacuum Pump, Tubes, and Bottles
For optimal operation of the 405 TS, all tubing, cables, and fittings for the fluid
supply and waste systems must be properly connected. This image illustrates
the rear panel of the instrument and the locations of the ports and connections
for the fluid supply and waste systems.
Rear Panel
Follow the Direct Drain Waste System Instructionsprovided with if you
purchased this accessory (in lieu of the standard waste system described below).
405™ TS Microplate Washer

Setting Up the 405 TS| 15
Waste System
Caution! Pump Installation. Do not plug the vacuum pump cable into a wall
outlet! Use the adapter provided with the pump to connect it to the Accessory
Outlet on the back of the instrument. This allows the 405 TS to regulate the
pump, turning it on and off as specified by the protocol.
When using a standard pump (rather than the high flow pump), set the
instrument’s Vacuum Dissipation Delay to prevent the pump from drawing
excess current and blowing the 5-amp fuse. See Define Instrument Settings on
page 22
lNote: The waste tubes have colored bands that match similarly colored
dots next to the inlet/outlet ports on the waste bottle caps to ensure the
correct connection of the tubing.
Waste System
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16 | 405 TS Getting Started Guide
Three lengths of tubing are shipped with the waste module:
Tubing: Connects:
Short tube with yellow and green bands gThe two waste bottles to each other
Long tube with green bands on both ends gBottle without sensor to vacuumport
Long tube with yellow and orange bands gBottle with waste sensor to the vacuum
pump
1. Locate the quick-release caps shipped inside the waste bottles and attach the
tubing to them as follows:
2. Connect the waste bottles to each other using the shortest length of tubing,
matching the colored bands on the tubing to colored dots on the caps.
3. Attach the waste sensor cable to the Waste Sensor port on the back of the
washer.
4. Attach the tube from the waste bottle with the waste sensor in its cap to the
vacuum pump.
5. Attach the tube from the waste bottle that does NOT have the waste sensor
in its cap to the Vacuum port on the back of the instrument.
6. Important!When installing BioTek’s vacuum pump, connect the pump’s AC
power cable to the vacuum pump Accessory Outlet on the back of the
instrument. (Use the accessory outlet adapter provided, if applicable.)
7. Place the waste bottles and vacuum pump on the same horizontal plane as the
instrument or below it, such as the floor beneath the work surface. This will
help optimize performance.
8. Make sure the waste bottle’s caps are well sealed.
lBioTek strongly recommends installing the vacuum line filter to protect
your vacuum pump.
Install the Vacuum Line Filter
The optional vacuum line filter (PN 48294) can be installed halfway between the
last waste bottle (overflow bottle) and the vacuum pump.
To do this, cut the tubing and insert the filter, noting the direction of flow. The flow
arrow on the filter should point toward the vacuum pump.
In the event of a fluid overflow, the filter should prevent the destruction of the
vacuum pump’s internal components. If an overflow does occur, check the filter for
trapped fluid. If fluid is found in the filter, remove the filter and drain using the
small white nut on top of the filter. Tighten the white nut and reinstall the filter.
405™ TS Microplate Washer

Setting Up the 405 TS| 17
Install the Fluid Supply System
Prepare the supply bottles and tubing:
1. Remove the Quick Release Connector
from its bag inside the supply bottle
and connect it to the raw supply
tubing provided.
lYou may need to cut the tubing to the
desired length.
2. Snap the connector into the top of the
supply bottle.
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18 | 405 TS Getting Started Guide
Make sure you hear the
connector click!
Attach the labels provided
with the washer to identify
the buffer bottles.
Note: To avoid spilling fluid when refilling bottles or changing reagents, first release the
Quick Connector from the bottle cap, use a paper towel to sop up the few drops in the
cap. Then, refill the bottle.
With Buffer Switching (4 Buffers):
1. Place the four supply
bottles on the same
surface as the
instrument.
2. Connect the tubing from
one of the supply bottles
to the “A” port on the
back of the washer.
3. Repeat step 2 with the
other three supply
bottles for “B,” “C,” and
“D” Buffers.
405™ TS Microplate Washer