Bio-Tek Synergy LX User manual

Synergy LX
Multi-Mode Microplate Reader
User Manual
ERRATA NOTICE: This document contains references to BioTek.
Please note that BioTek is now Agilent. For more information,
go to www.agilent.com/lifesciences/biotek.

Synergy LX
Multi-Mode Microplate Reader
User Manual
ERRATA NOTICE: This document
contains references to BioTek.
Please note that BioTek is now Agilent.
For more information, go to
www.agilent.com/lifesciences/biotek.
Document part number 1501000N, revision A
BioTek Instruments, Inc.
December 2021

Contents
Contents 2
Notices 5
Contact Information 6
Intended Use Statement 7
Quality Control 7
Warranty and Product Registration 7
Safety Notices 8
Warnings and Precautions 8
Symbols 12
Conformance to Standards 22
EMC Information and Technical Description 23
Ingress Protection Code 23
Disposal 23
Introduction 1
Product Description 2
Package Contents 3
Optional Accessories 4
Technical Support 6
Installation 7
Important Information 8
1. Unpack the Box and See What’s Inside 9
2. Remove the Shipping Screw and Insert the Light-Blocking Plug 10
3. (Gen5 control only) Prepare the Host Computer 12
4. (Gen5 control only) Connect the Host Computer andReader 13
5. Install the Power Supply 13
6. Turn on the Reader and Run the Power-Up System Test 15
7. (Gen5 control only) Start Gen5 and Test Communication 15
Operational/Performance Qualification 16
Repackaging and Shipping Instructions 17
2|Contents
Synergy LX User Manual
1501000N Revision A
BioTek Instruments, Inc.

Contents|3
Getting Started 23
External Components 24
Filter Cubes 26
Operate the Reader Using the Touchscreen 34
Run a Take3 Session Using the Touchscreen 45
Getting Started with Gen5 Software 48
Recommendations for Optimum Performance 50
Periodic Maintenance 52
Maintenance Overview 53
Warnings and Precautions 54
Clean Exposed Surfaces 55
Clean the Touchscreen 55
Inspect/Clean Filter Cubes 56
Clean the Air Filter 57
Replace the Halogen Bulb Assembly 59
Decontamination 60
Instrument Qualification Process 62
System Test 63
Plate Shaker Test 63
Absorbance Testing 64
FluorescenceTesting 73
Luminescence Test 81
Instrument Qualification Procedures 85
Overview 86
IQ/OQ/PQ Description 86
Recommended Qualification Schedule 88
System Test 89
Absorbance Plate Test 90
Absorbance Liquid Tests 92
Fluorescence Plate Tests 98
Fluorescence Liquid Tests 98
Synergy LX User Manual
1501000N Revision A
BioTek Instruments, Inc.

Luminescence Test 106
Specifications 108
General Specifications 109
Absorbance Specifications 110
Fluorescence Specifications 112
Luminescence Specifications 112
Sample Reports 113
Sample System Report 114
Sample Absorbance Test Plate Report 116
Sample Assay Results 117
Error Conditions 119
Overview 120
Error Codes 120
Safety Information 146
Safety Notices 147
In This Book 174
4|Contents
Synergy LX User Manual
1501000N Revision A
BioTek Instruments, Inc.

Notices|5
Notices
All Rights Reserved
© 2021, BioTek Instruments, Inc. No part of this publication may be reproduced,
transcribed, or transmitted in any form, or by any means electronic or mechanical,
including photocopying and recording, for any purpose other than the purchaser’s use
without written permission of BioTek Instruments, Inc.
Restrictions and Liabilities
Information in this document is subject to change and does not represent a commitment
by BioTek Instruments, Inc. Changes made to the information in this document will be
incorporated in new editions of the publication. No responsibility is assumed by BioTek for
the use or reliability of software or equipment that is not supplied by BioTek or its affiliated
dealers.
Synergy LX User Manual
1501000N Revision A
BioTek Instruments, Inc.

Contact Information
Agilent Technologies, Inc.
5301 Stevens Creek Blvd.
Santa Clara, CA 95051
Worldwide Sales and Support
www.agilent.com/en/contact-us/page
Technical Support and Service
www.agilent.com/en/support
[email protected]
Instrument service and repair is available worldwide at one of our international service
centers and in the field at your location.
UK Responsible Person (UKRP)
Agilent LD UK Ltd
5500 Lakeside
Cheadle Royal Business Park
Cheadle, Cheshire SK8 3GR
6|Contact Information
Synergy LX User Manual
1501000N Revision A
BioTek Instruments, Inc.

Intended Use Statement|7
Intended Use Statement
The Synergy LX is a multi-mode microplate reader and intended to be used for the
examination of specimens to analyze their characteristics and impact on a variety of analytes.
Quality Control
It is considered good laboratory practice to run laboratory samples according to instructions
and specific recommendations included in the assay package insert for the test to be
conducted. Failure to conduct Quality Control checks could result in erroneous test data.
Warranty and Product Registration
Review the warranty information that shipped with your product. Register your product to
ensure you receive important information updates about the products you have purchased.
Synergy LX User Manual
1501000N Revision A
BioTek Instruments, Inc.

Safety Notices
Raadpleeg Bijlage D voor informatie in andere talen.
Reportez-vous à l'annexe D pour obtenir des informations dans d'autres langues.
Informationen in anderen Sprachen finden Sie in Anhang D.
Fare riferimento all'Appendice D per informazioni in altre lingue.
Consulte el Apéndice D para obtener información en otros idiomas.
Pay special attention to the following safety notices in all product documentation.
AWARNING notice denotes a hazard. It calls attention to an operating procedure,
practice, or the like that, if not correctly performed or adhered to, could result in
personal injury or death. Do no proceed beyond a WARNINGnotice until the
indicated conditions are fully understood and met.
ACAUTION notice denotes a hazard. It calls attention to an operating procedure,
practice, pr the like that, if not correctly performed or adhered to, could result in
damage to the product or loss of important data. Do not proceed beyond a
CAUTIONnotice until the indicated conditions are fully understood and met.
Warnings and Precautions
Electrical Hazards
Internal Voltage. Always turn off the power switch and unplug the
power supply before cleaning the outer surface of the instrument.
Power Rating. The instrument’s power supply or power cord must be
connected to a power receptacle that provides voltage and current
within the specified rating for the system. Use of an incompatible power
receptacle may produce electrical shock and fire hazards.
Electrical Grounding. Never use a plug adapter to connect primary
power to the external power supply. Use of an adapter disconnects the
utility ground, creating a severe shock hazard. Always connect the
power cord directly to an appropriate receptacle with a functional
ground.
Service. Only qualified technical personnel should perform service
procedures on internal components.
Power Supply. Use only the power supply shipped with the instrument,
and operate it within the range of line voltages listed on it.
8|Safety Notices
Synergy LX User Manual
1501000N Revision A
BioTek Instruments, Inc.

Warnings and Precautions|9
Chemical/Environmental
Potential Biohazards. Some assays or specimens may pose a biohazard.
Adequate safety precautions should be taken as outlined in the assay’s
package insert. Always wear safety glasses and appropriate protective
equipment, such as chemical-resistant rubber gloves and apron.
Liquids. Avoid spilling liquids on the instrument; fluid seepage into internal
components creates a potential for shock hazard or instrument damage. If
a spill occurs while a program is running, stop the program and turn off the
instrument. Wipe up all spills immediately. Do not operate the instrument
if internal components have been exposed to fluid.
Liquids. Do not immerse the instrument, spray it with liquid, or use a
dripping-wet cloth on it. Do not allow water or other cleaning solution to
run into the interior of the instrument. If this happens, contact Technical
Support. Do not soak the touchscreen.
Environmental Conditions. Do not expose the instrument to temperature
extremes. For proper operation, temperature near the instrument should
remain within the range in the Specifications section of this document.
Performance may be adversely affected if temperatures fluctuate above or
below this range
Sodium Hypochlorite. Do not expose any part of the instrument to the
recommended diluted sodium hypochlorite solution for more than 20
minutes. Prolonged contact may damage the instrument surfaces. Be
certain to rinse and thoroughly wipe all surfaces.
Lubricants. Do not apply lubricants to moving parts. Lubricant on
components in the carrier compartment will attract dust and other
particles, which may cause the instrument to produce an error.
Components
Hot Surface. The lamp assembly is hot when the instrument is turned on.
Turn off the reader and allow the bulb to cool for at least 15 minutes
before attempting to replace it.
Accessories. Only accessories that meet the manufacturer's specifications
shall be used with the instrument.
Synergy LX User Manual
1501000N Revision A
BioTek Instruments, Inc.

Shipping Hardware. All shipping hardware must be removed before
operating the instrument and reinstalled before repackaging the
instrument for shipment.
Filter Cube (F models). The reader’s internal filter cube table must exactly
match the contents of the installed filter cube. Gen5 users: The Gen5
software filter cube table must exactly match the contents of the filter
cube.
If you exchange the filter cube or modify its contents, you must update the
filter cube table(s).
The filter cube is accessed through a hinged door in the front of the
instrument. Do not open the door to access the filter cube during
instrument operation! Doing so may result in invalid data.
Touchscreen. Use your fingertip to operate the touchscreen. Do not use a
sharp stylus or pencil on the touchscreen. Doing so will damage the
touchscreen's surface. You can use a stylus designed for resistive
touchscreens.
Touchscreen. Avoid strong solvents, such as alcohol, acetone, ammonium
chloride, methylene chloride, and hydrocarbons. These will permanently
damage the touchscreen. Avoid fibrous materials, such as paper towels,
which can scratch the touchscreen. Dirt particles and cleaning agents will
get trapped in the scratches. Never spray solutions directly on the
touchscreen.
Spare Parts. Only approved spare parts should be used for maintenance.
The use of unapproved spare parts and accessories may result in a loss of
warranty and potentially impair instrument performance or cause damage
to the instrument.
Service. Only qualified technical personnel should perform service
procedures on internal components.
Intended Product Use
Software Quality Control. The operator must follow the manufacturer’s
assay package insert when modifying software parameters and establishing
reading methods. It is considered good laboratory practice to run
laboratory samples according to instructions and specific
recommendations included in the assay package insert for the test to be
conducted. Failure to conduct quality control checks could result in
erroneous test data.
10 |Warnings and Precautions
Synergy LX User Manual
1501000N Revision A
BioTek Instruments, Inc.

Warnings and Precautions|11
Data Reduction. No limits are applied to the raw measurement data. Data
exported via computer control must be analyzed by the operator. The
performance characteristics of the data reduction software have not been
established with any laboratory diagnostic assay. Users must evaluate this
instrument and PC-based software in conjunction with their specific assay
(s). This evaluation must include the confirmation that performance
characteristics for the specific assay(s) are met.
Unspecified Use. Failure to operate equipment according to the guidelines
and safeguards specified in the product user documentation could result in
a hazardous condition.
Use of labware other than described in this document can result in
positioning errors during program execution.
Synergy LX User Manual
1501000N Revision A
BioTek Instruments, Inc.

Symbols
Veiligheidssymbolen
Symboles de sécurité
Sicherheitssymbole
Simboli di sicurezza
Símbolos de seguridad
Caution, consult the instructions for use for important cautionary
information such as warnings and precautions
Voorzichtig, raadpleeg de gebruiksaanwijzing voor belangrijke
voorzorgsinformatie zoals waarschuwingen en voorzorgsmaatregelen
Attention, pour des informations de mise en garde importantes telles
que des avertissements et des précautions, consultez le mode d'emploi.
Achtung, lesen Sie die Gebrauchsanweisung für wichtige
Vorsichtshinweise wie Warnungen und Sicherheitsvorkehrungen
Attenzione, consultare le istruzioni per l'uso per importanti informazioni
cautelative come avvertenze e precauzioni
Precaución, consulte las instrucciones de uso para obtener información
importante, como advertencias y precauciones
Warning; Biological hazard
Waarschuwing; biologisch gevaar
Avertissement : Risque biologique
Warnung; biologische Gefahr
Avvertenza, rischio biologico
Advertencia: peligro biológico
12 |Symbols
Synergy LX User Manual
1501000N Revision A
BioTek Instruments, Inc.

Symbols|13
Warning; Pinch hazard
Waarschuwing; beknellingsgevaar
Avertissement : risque de pincement
Warnung; Quetschgefahr
Avvertenza, rischio di pizzicamento
Advertencia: peligro de atrapamiento
Warning; Hot surface
Waarschuwing; heet oppervlak
Avertissement : surface chaude
Warnung; heiße Oberfläche
Avvertenza, superficie molto calda
Advertencia: superficie caliente
Synergy LX User Manual
1501000N Revision A
BioTek Instruments, Inc.

Disposal Notice: Dispose of the instrument according to Directive
2012/19/EU, “on waste electrical and electronic equipment (WEEE)” or
local ordinances
Kennisgeving van verwijdering: Verwijder het instrument volgens
Richtlijn 2012/19/EU betreffende afgedankte elektrische en
elektronische apparatuur (AEEA) of lokale verordeningen
Avis concernant la mise au rebut : mettez l'instrument au rebut
conformément à la directive 2012/19/EU portant sur les déchets
d'équipement électrique et électronique (DEEE) ou aux dispositions
locales.
Entsorgungshinweis: Entsorgen Sie das Gerät gemäß der Richtlinie
2012/19/EU „für Elektro- und Elektronik-Altgeräte (WEEE)“bzw. den
Landesvorschriften.
Avviso per lo smaltimento: smaltire lo strumento in base alla Direttiva
2012/19/EU, sui "rifiuti di apparecchiature elettriche ed elettroniche
(WEEE)" o le ordinanze locali
Aviso de eliminación: elimine el instrumento de conformidad con la
Directiva 2012/19/UE sobre residuos de aparatos eléctricos y
electrónicos (RAEE) o las ordenanzas locales
14 |Symbols
Synergy LX User Manual
1501000N Revision A
BioTek Instruments, Inc.

Symbols|15
CE Marking CE Marking – Indicates compliance with the requirements of
the Directive 2014/30/EU on Electromagnetic Compatibility and the
Directive 2014/35/EU on Low Voltage
CE-markering – Geeft aan dat wordt voldaan aan de vereisten van
Richtlijn 2014/30/EU inzake elektromagnetische compatibiliteit en
Richtlijn 2014/35/EU inzake laagspanning
Marquage CE – Indique la conformité aux exigences de la directive
2014/30/UE sur la compatibilité électromagnétique et de la directive
2014/35/UE sur la basse tension
CE-Kennzeichnung – Zeigt die Einhaltung der Anforderungen der
Richtlinie 2014/30/EU über elektromagnetische Verträglichkeit und der
Richtlinie 2014/35/EU über Niederspannung
Marcatura CE – Indica la conformità ai requisiti della Direttiva
2014/30/UE sulla Compatibilità Elettromagnetica e della Direttiva
2014/35/UE sulla Bassa Tensione
Marcado CE: indica el cumplimiento de los requisitos de la Directiva
2014/30 / UE sobre compatibilidad electromagnética y la Directiva
2014/35 / UE sobre baja tensión.
Date of manufacture
Productiedatum
Date de fabrication
Herstellungsdatum
Data di produzione
Fecha de fabricación
TÜV SÜD Certification Mark – Type tested; production monitored
TÜV SÜD certificeringsmerk - type getest; productie bewaakt
TÜV SÜD Marque de certification – Type testé ; production contrôlée
TÜV SÜD-Prüfzeichen – Typ geprüft; Produktion überwacht
Marchio di certificazione TÜV SÜD: tipo testato, produzione monitorata
Marca de certificación TÜV SÜD: tipo probado, producción controlada
Synergy LX User Manual
1501000N Revision A
BioTek Instruments, Inc.

This product complies with environmental protection use period as
defined in People’s Republic of China Electronic Industry Standard
SJ/T11364-2006. Toxic or hazardous substances will not leak or mutate
under normal operating conditions for 40 years.
Dit product voldoet aan de milieubeschermingsgebruiksperiode zoals
gedefinieerd in de Electronic Industry Standard SJ/T11364-2006 van de
Volksrepubliek China. Giftige of gevaarlijke stoffen zullen onder normale
bedrijfsomstandigheden gedurende 40 jaar niet lekken of muteren.
Ce produit est conforme à la période d'utilisation dans le cadre de la
protection de l'environnement telle que définie par la norme de
l'industrie électronique de la République populaire de Chine SJ/T11364-
2006. Les substances toxiques ou dangereuses ne fuiront pas ou ne
subiront pas de mutation dans des conditions de fonctionnement
normales pendant 40 ans.
Dieses Produkt entspricht der Umweltschutz-Nutzungsdauer gemäß der
Definition im Electronic Industry Standard SJ/T11364-2006 der
Volksrepublik China. Giftige oder gefährliche Stoffe werden unter
normalen Betriebsbedingungen 40 Jahre lang nicht austreten oder
mutieren.
Questo prodotto è conforme al periodo di utilizzo della protezione
ambientale come definito nello Standard del settore elettronico della
Repubblica Popolare Cinese SJ/T11364-2006. Le sostanze tossiche o
pericolose non fuoriescono o non subiscono mutazioni in condizioni
operative normali per 40 anni.
Este producto cumple con el periodo de uso de protección ambiental
según el estándar SJ/T11364-2006 de la República Popular China para la
industria electrónica. Las sustancias tóxicas o peligrosas no se filtrarán ni
mutarán en condiciones de funcionamiento normales durante 40 años.
16 |Symbols
Synergy LX User Manual
1501000N Revision A
BioTek Instruments, Inc.

Symbols|17
UK Conformity Assessed marking is a certification mark that indicates
conformity with the applicable requirements for products sold within
Great Britain.
De 'UK Conformity Assessed'-markering is een certificeringsmerk dat
aangeeft dat producten die in Groot-Brittannië worden verkocht,
voldoen aan de toepasselijke eisen.
Le marquage UK Conformity Assessed est une marque de certification qui
indique la conformité aux exigences applicables aux produits vendus en
Grande-Bretagne.
Die Kennzeichnung „UK Conformity Assessed“ist ein
Zertifizierungszeichen, das die Konformität mit den geltenden
Anforderungen für in Großbritannien verkaufte Produkte anzeigt.
Il marchio UKCA (conformità valutata del Regno Unito) è un marchio di
certificazione che indica la conformità ai requisiti applicabili per i prodotti
venduti in Gran Bretagna.
El marcado UKCA (UK Conformity Assessed) es una marca de certificación
que indica la conformidad con los requisitos aplicables para los
productos vendidos en Gran Bretaña.
Synergy LX User Manual
1501000N Revision A
BioTek Instruments, Inc.

EAC-MED is a certification mark to indicate products that conform to all
the safety and quality requirements of the Eurasian Customs Union. It
means that the EAC-MED marked products meet all requirements of the
corresponding technical regulations and have passed all conformity
assessment procedures.
EAC-MED is een certificeringsmerk om producten aan te duiden die
voldoen aan alle veiligheids- en kwaliteitseisen van de Euraziatische
douane-unie. Dit betekent dat de producten met een EAC-MED-
markering aan alle eisen van de desbetreffende technische voorschriften
voldoen en alle conformiteitsbeoordelingsprocedures hebben doorlopen.
EAC-MED est une marque de certification qui indique la conformité des
produits à toutes les exigences de sécurité et de qualité de l'Union
douanière eurasiatique. Cela signifie que les produits marqués EAC-MED
satisfont à toutes les exigences des réglementations techniques
correspondantes et ont passé toutes les procédures d'évaluation de la
conformité.
EAC-MED ist ein Zertifizierungszeichen zur Kennzeichnung von
Produkten, die allen Sicherheits- und Qualitätsanforderungen der
Eurasischen Zollunion entsprechen. Das bedeutet, dass die EAC-MED-
gekennzeichneten Produkte alle Anforderungen der entsprechenden
technischen Bestimmungen erfüllen und alle
Konformitätsbewertungsverfahren bestanden haben.
EAC-MED è un marchio di certificazione che indica prodotti conformi a
tutti i requisiti di sicurezza e qualità dell'Unione doganale eurasiatica. Ciò
significa che i prodotti con marchio EAC-MED soddisfano tutti i requisiti
dei regolamenti tecnici corrispondenti e hanno superato tutte le
procedure di valutazione della conformità.
EAC-MED es una marca de certificación para indicar productos que
cumplen con todos los requisitos de seguridad y calidad de la Unión
Aduanera Euroasiática. Significa que los productos con la marca EAC MED
cumplen todos los requisitos de los reglamentos técnicos
correspondientes y han superado todos los procedimientos de
evaluación de conformidad.
18 |Symbols
Synergy LX User Manual
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Symbols|19
Product complies with Australian Communications Requirements
EESS - The Regulatory Compliance Mark (RCM)
ACMA Labeling Requirements
Product voldoet aan de Australische communicatie-eisen
EESS - De markering voor naleving van de regelgeving (RCM)
ACMA-etiketteringsvoorschriften
Le produit est conforme aux exigences australiennes en matière de
communication
EESS - Marque réglementaire de conformité (RCM)
Exigences en matière d'étiquetage ACMA
Das Produkt entspricht den australischen
Kommunikationsanforderungen.
EESS – Kennzeichnung „Regulatory Compliance Mark“(RCM)
ACMA-Kennzeichnungsanforderungen
Il prodotto è conforme ai requisiti Australian Communications
Requirements
EESS: marchio di conformità alle normative
Requisiti di etichettatura ACMA
El producto cumple con los requisitos de comunicaciones de Australia.
EESS: marcado RCM (Regulatory Compliance Mark) de cumplimiento de
la normativa.
Requisitos de etiquetado de ACMA
Synergy LX User Manual
1501000N Revision A
BioTek Instruments, Inc.