Bio-Tek Synergy HT User manual

Operator’s Manual
Multi-Mode Microplate Reader
Synergy™HT

Synergy™ HT
Multi-Mode Microplate Reader
Operator’s Guide
November 2008
© 2008
Part Number 7091000
Revision I
BioTek® Instruments, Inc.

ii | Preface
BioTek Instruments
Notices
BioTek® Instruments, Inc.
Highland Park, P.O. Box 998
Winooski, Vermont 05404-0998 USA
All Rights Reserved
© 2008, BioTek® Instruments, Incorporated. No part of this publication may be
reproduced, transcribed, or transmitted in any form, or by any means electronic or
mechanical, including photocopying and recording, for any purpose other than the
purchaser’s use without written permission of BioTek Instruments, Inc.
Trademarks
BioTek® is a registered trademark, and Synergy™ HT, Gen5™, KC4™, and BioStack™
are trademarks of BioTek Instruments, Inc. All other trademarks are the property of
their respective holders.
Restrictions and Liabilities
Information in this document is subject to change and does not represent a
commitment by BioTek Instruments, Inc. Changes made to the information in this
document will be incorporated in new editions of the publication. No responsibility is
assumed by BioTek for the use or reliability of software or equipment that is not
supplied by BioTek or its affiliated dealers.

Contents | iii
Synergy HT Operator’s Manual
Contents
Notices ...........................................................................................ii
Contact Information ....................................................................... vii
Revision History ............................................................................ viii
Document Conventions .................................................................. viii
Intended Use Statement ................................................................ xiii
Quality Control ............................................................................. xiii
Warranty & Product Registration...................................................... xiii
Warnings ..................................................................................... xiv
Hazards and Precautions ................................................................ xiv
Directives..................................................................................... xvi
Electromagnetic Interference and Susceptibility ................................ xvii
User Safety ................................................................................. xvii
Safety Symbols............................................................................xviii
Chapter 1: Introduction.......................................................................1
Synergy™ HT Multi-Mode Microplate Reader........................................ 2
Features ........................................................................................ 3
Package Contents............................................................................ 4
Optional Accessories........................................................................ 5
Product Support & Service................................................................ 6
Technical Assistance Center (TAC) ................................................ 6
Returning Instruments for Service/Repair ...................................... 6
Contacting BioTek for Applications Support .................................... 6
Chapter 2: Installation ........................................................................7
1: Unpack and Inspect the Reader .................................................... 8
2: Remove the Shipping Panel ........................................................10
3: Remove the Microplate Carrier Shipping Screw ..............................12
4: Install the Fluorescence Lamp Assembly .......................................13
5: Select an Appropriate Location ....................................................14
6: Connect the Power Supply ..........................................................15
7: Unpack and Inspect the Dispense Module .....................................16
8: Install the Dispense Module ........................................................19
9: Connect the Host Computer ........................................................23
10: Install Software on the Host Computer .......................................24
11: Turn on the Reader..................................................................24
12: Establish Communication ..........................................................25
Using Gen5 ..............................................................................26
Using KC4 ................................................................................26
13: Run a System Test ..................................................................27
14: Test the Injector System ..........................................................28
Register with BioTek .......................................................................30

iv | Preface
BioTek Instruments
Operational/Performance Qualification...............................................30
Repackaging and Shipping Instructions .............................................31
Chapter 3: Getting Started ................................................................35
Key Components............................................................................36
Power Switch, Carrier Eject Button, Microplate Carrier ....................36
Lamp Assembly and Filter Wheel Access .......................................37
Excitation and Emission Filter Wheels ...........................................38
Installing the Time-Resolved Fluorescence Cartridge ......................41
Configuring the System for Luminescence Measurements................42
The External Dispense Module.....................................................43
Gen5 Software...............................................................................46
Viewing/Updating the Filter and Wavelengths Tables ......................46
Creating Protocols and Experiments .............................................47
Controlling the Dispense Module..................................................50
KC4 Software ................................................................................52
Viewing/Updating the Filter and Wavelengths Tables ......................52
Creating Protocols .....................................................................53
Reading Plates ..........................................................................55
Controlling the Dispense Module..................................................56
Recommendations for Achieving Optimum Performance .......................59
Chapter 4: Instrument Qualification..................................................61
Overview ......................................................................................62
IQ/OQ/PQ .....................................................................................62
Recommended Qualification Schedule ...............................................64
System Test ..................................................................................65
Description...............................................................................65
Procedure ................................................................................66
Absorbance Plate Test.....................................................................70
Description...............................................................................70
Test Plate Certificates ................................................................71
Setup: Gen5.............................................................................72
Procedure: Gen5 .......................................................................73
Setup: KC4 ..............................................................................74
Procedure: KC4.........................................................................74
Results & Troubleshooting Tips....................................................78
Absorbance Liquid Tests..................................................................80
Stock Solution Formulation ..............................................................81
Liquid Test 1.............................................................................82
Liquid Test 2.............................................................................83
Liquid Test 3.............................................................................86
Fluorescence Tests .........................................................................89
Required Materials.....................................................................90
Test Solutions...........................................................................91

Contents | v
Synergy HT Operator’s Manual
Procedure ................................................................................92
Results Analysis ........................................................................92
Troubleshooting ........................................................................93
Pipette Map ..............................................................................94
Gen5 Protocol Reading Parameters ..............................................95
KC4 Protocol Reading Parameters ................................................96
Fluorescence Tests Using Methylumbelliferone...............................98
Dispense Module Tests.................................................................. 102
Required Materials................................................................... 103
Test Solution Recipes............................................................... 104
Test Setup: Gen5 .................................................................... 105
Test Setup: KC4...................................................................... 105
Test Procedure: Gen5 .............................................................. 106
Test Procedure: KC4 ................................................................ 108
Results Analysis ...................................................................... 109
Creating Test Protocols Using Gen5............................................ 110
Creating Test Protocols Using KC4 ............................................. 115
Dispense Accuracy & Precision Test Worksheet ................................. 121
Chapter 5: Preventive Maintenance.................................................123
Recommended Maintenance Schedule ............................................. 124
Overview ............................................................................... 124
Dispense Module ..................................................................... 124
Schedule................................................................................ 126
Warnings & Precautions ................................................................ 126
Cleaning Exposed Surfaces ............................................................ 127
Inspecting/Cleaning Excitation and Emission Filters ........................... 128
Flushing/Purging the Fluid Path ...................................................... 129
Running a Dispense Protocol (Optional) ........................................... 130
Emptying/Cleaning the Tip Priming Trough....................................... 132
Cleaning the Priming Plate............................................................. 132
Cleaning the Internal Components .................................................. 133
Required Materials................................................................... 134
Removing the Reader’s Shroud.................................................. 135
Removing the Internal Tubes and Injector Heads ......................... 137
Cleaning the Internal Tubes and Injector Heads........................... 140
Cleaning the Optical Probes ...................................................... 141
Cleaning the Reader’s Internal Surface ....................................... 149
Reassembling the Components.................................................. 150
Performance Check.................................................................. 151
Appendix A: Decontamination .........................................................153
Purpose ...................................................................................... 154
Required Materials........................................................................ 155
Procedure for Models without Injectors............................................ 156

vi | Preface
BioTek Instruments
Routine Procedure for Models with Injectors..................................... 157
Clean Exposed Surfaces ........................................................... 157
Decontaminate the Fluid Lines................................................... 158
Rinse the Fluid Lines................................................................ 159
Clean the Internal Tubing and Injector Heads .............................. 159
Clean the Tip Prime Trough and Priming Plate ............................. 160
Alternate Procedure for Models with Injectors................................... 161
Appendix B: Computer Control ........................................................163
Appendix C: Error Codes..................................................................165
Appendix D: Specifications ..............................................................207
Appendix E: Instrument Dimensions for Robotic Interface..............215

Contact Information | vii
Synergy HT Operator’s Manual
Contact Information
 For more detailed information on contacting BioTek for product support
and service, turn to page 6.
BioTek® Instruments, Inc.
Highland Park, P.O. Box 998
Winooski, Vermont 05404-0998 USA
Customer Service and Sales
Internet: www.biotek.com
Phone: [phone] (toll free in the U.S.)
802-655-4740 (outside the U.S.)
Fax: [phone]
E-Mail: [email protected]
Service/TAC
Phone: [phone] (toll free in the U.S.)
802-655-4740 (outside the U.S.)
Fax: [phone]
E-Mail: [email protected]
European Coordination Center/
Authorized European Representative
BioTek® Instruments GmbH
Kocherwaldstrasse 34
D-74177 Bad Friedrichshall
Germany
Internet: www.biotek.de
Phone: [phone]
Fax: [phone]
E-Mail: [email protected]

viii | Preface
BioTek Instruments
Revision History
Rev Date Changes
A 04/2002 First issue
B 08/2002 Added Time-Resolved Mode
C 08/2003 Added Dual Fluid Dispense Feature
Ch. 1, Introduction: Updated specifications, accessories, and technical
support.
Ch. 2, Instrument Description: Updated component descriptions and
added drawings.
Ch. 3, Installation: Revised Dispenser Module setup instructions and
KC4 launch procedure. Revised unpacking/repackaging instructions.
Added new Chapter 4, “Getting Started With KC4.”
Ch. 5, Performance Verification/Qualification Tests (formerly Chapter 4):
Revised test procedures.
Reformatted Appx A, Decontamination, and Appendix B, Computer
Control.
Updated Appx C, Error Codes.
Renamed Appx D, Microplate Location Dimensions to “Instrument
Dimensions.”
D 12/2003 Preface: Updated safety symbols and text (p. ix and x). Updated
Intended Use Statement (p. xi). Revised Warranty (p. xii).
Chapter 1: Modified Introduction (p. 1-3). Updated list of optional
accessories (p. 1-4). Revised absorbance reading speed information (p.
1-5). Clarified fluorescence specifications (p. 1-8 and 1-9). Added
specifications for injector model (p. 1-10).
Chapter 2: Clarified description of external and internal components of
the injector model and updated drawings. Moved procedure for replacing
the lamp assembly to Ch. 6.
Chapter 5: Added note regarding the availability of the Installation-
Operational-Performance (IQ-OQ-PQ) package (PN 7090521) (p. 5-2).
Updated liquid test procedures.
Added new Chapter 6, Maintenance and Troubleshooting, which
includes: Sample reproducible page from maintenance logbook;
Procedures for maintenance and routine cleaning; Instructions for
changing injector positions.
Updated decontamination procedure (Appendix A).
Modified Appendix B, Computer Control.
Corrected Appendix C, Error Codes.
Changed “Dispenser Module” to “Dispense Module” throughout.
E 02/2004 Chapter 1, Introduction: Removed reference to “NB” version (p. 1-3).
Added specifications to reflect the use of an additional PMT type
(R4220PHA) to Hardware Features (p. 1-3). Moved filter plug (7082073)
from Optional Accessories list to Package Contents (p. 1-4). Added
required specifications for microplates used in “Luminescence” mode

Revision History | ix
Synergy HT Operator’s Manual
Rev Date Changes
(E) (p. 1-5). Added “6- to 96-well plates” and “fluorescence and
luminescence read modes” to Injector Model features (p. 110).
Removed “option” from reference to Incubation specifications (p. 1-11).
Chapter 3, Installation: Removed references to “NB” version from
description of reader model options (Setting Communication Parameters
in KC4, p. 3-12).
Chapter 5, Performance Verification/Qualification Tests: Updated Figure
5-1, Sample System Test (p. 5-6 and 5-7).
Updated Appendix C, Error Codes.
F 07/2004 General: Edited and reformatted text according to new template. Added
photographs for clarification as needed.
Chapter 1: Updated Optional Accessories list.
Chapter 2: Updated text and graphics to describe/illustrate: Removal of
the front injector. Redesign of the priming plate, to limit splatter.
Improved system design to reduce need for periodic maintenance.
Elimination of the right-front tip priming trough, and redesign of the
left-rear trough. Improved bottle holder setup.
Chapter 3: Updated diagram showing removal of Dispense Module from
inner shipping box. Updated procedure for setting up Dispense Module
on the Injector Model.
Chapter 4: Removed instructions for setting injector position.
Chapter 6: Added preventive maintenance procedure for periodic
cleaning of the top/bottom fluorescence optical probes and the
absorbance read channel optical path.
Appendix A: Updated drawing of tip priming trough and priming plate.
G 8/2005 Updated Warranty information.
Moved “Specifications” from Chapter 1 to Appendix D. Corrected
operating temperature (18-40°C) and injector accuracy (±1 µl at 5-50
µl) to match published specifications.
Removed Chapter 2, “Instrument Description” and distributed the
information and photos among the remaining chapters.
Reorganized the flow of the “Installation” chapter to better represent
actual practice. Added test to verify the injector system setup.
Changed the former “Getting Started with KC4” chapter to a broader
“Getting Started” chapter that includes information on the key
instrument components. Added new topic for configuring the system for
luminescence reads.
Renamed the “Performance Verification/Qualification Tests” chapter to
“Instrument Qualification.” Replaced former Dispense Precision &
Accuracy Tests with new tests that use a single green dye solution and a
single microplate.
Restructured the “Preventive Maintenance” chapter to better represent
actual practice. Added a recommended maintenance table for models
without injectors. Added new photos to help with identification of the
various components.
Updated the “Error Codes” appendix with recent information.
Additional minor corrections and improvements throughout.

x | Preface
BioTek Instruments
Rev Date Changes
H 5/06 Redesigned the front cover. Removed unnecessary Warranty
information; a Warranty card ships with every instrument. Added
warning to shut down instrument and wait for the fluorescence lamp to
cool down before replacing it. Added the PN for a replacement
fluorescence lamp (7080500).
For models with injectors: Simplified the installation and setup steps for
the Dispense Module. Added recommendation to set a tip prime volume
equal to the per-well dispense volume for volumes < 20 µl.
Updated Absorbance Plate Test instructions related to Peak Wavelength,
to support the modified 7-filter test plate.
Simplified the process for creating Titration Dyes for the Fluorescence
(SF) Sensitivity Test. Added information to the pass/fail criteria table for
the (SF) Sensitivity Test.
Clarified that for models without injectors, the reader’s internal chamber
and optical probes are not user-accessible for cleaning.
Updated the “Error Codes” appendix with recent information.
Additional minor cosmetic changes throughout.
Added/modified instructions throughout to support Gen5™, including:
Chapter 2, Installation - Added instructions for installing software,
establishing communication with the reader, installing/testing dispense
module components.
Chapter 3, Getting Started - Added introductory information for new
Gen5 users.
Chapter 4, Instrument Qualification - Added instructions for performing
the System Test, Absorbance Plate Test, and Dispense Accuracy &
Precision Test.
Chapter 5, Preventive Maintenance - Added instructions for creating the
optional Dispense protocol in Gen5.
I 11/2008 Throughout: Changed product description from “Multi-Detection” to
“Multi-Mode”. Changed “Bio-Stack” to “BioStack.”
Preface: Corrected Service/TAC fax number. Updated the Intended Use
section with respect to IVD labeling. Added cautions for Electromagnetic
Environment and Compatibility. Updated Directives. Added ‘Pinch
hazard’ to Hazards and Precautions. Added ‘Consult Instructions for use’
and ‘IVD’ to Safety Symbols.
Chapter 1, Introduction: In the product introduction section, added note
that Synergy HT basecode software version 2.24 or greater is required
for use with Gen5™. Under ‘Package Contents’ added notice that part
numbers are subject to change over time, and updated part numbers for
the priming plate and tip priming trough.
Chapter 2, Installation: Added section “Product Registration.”
Chapter 4, Instrument Qualification: Modified sample System Test
Report and Absorbance Test Plate Results to reflect more current date
and minimum basecode for Synergy HT to work with Gen5. Absorbance
Liquid Tests section: Liquid Test 3, removed instructions for creating the
rarely used Buffer Solution A. Fluorescence Liquid Tests section: Added
option to use Sodium Borate instead of PBS with sodium fluorescein.

Revision History | xi
Synergy HT Operator’s Manual
Rev Date Changes
(I) Reconfigured the SF test solutions, dilutions, and pipette maps for
efficiency and consistency with other BioTek products. Added option to
use Methylumbelliferone to test the top optics. Dispense Module Tests
section: Corrected the formula for Accuracy % Error.
Chapter 5, Preventive Maintenance: Removed unnecessary “Clean
Supply Bottle” section. Modified the Running a Dispense Protocol
procedure to include running the experiment and inspecting the plate.
Added a missing word to the Cleaning the Optical Probes section.
Appendix C, Error Codes: Corrected the range of error codes under
Home Sensor Initial Find Errors. Removed some text from the Status
String Format section that was misleading.

xii | Preface
BioTek Instruments
Document Conventions
This icon calls attention to important safety notes.
Warning! A Warning indicates the potential for bodily harm and
tells you how to avoid the problem.
Caution A Caution indicates potential damage to the instrument
and tells you how to avoid the problem.
Note: Bold text is primarily used for emphasis.
italic
Topics that apply only to specific Synergy HT models are
preceded by a notice in italics, for example: Applies only to
Synergy™ HT models with injectors.
 This icon calls attention to important information.

Intended Use Statement | xiii
Synergy HT Operator’s Manual
Intended Use Statement
The Synergy™ HT is a single-channel absorbance, fluorescence, and luminescence micro-
plate reader that uses a dual-optics design to perform measurements of samples in a
microplate format. The performance characteristics of the data reduction software have not
been established with any laboratory diagnostic assay. The user must evaluate this
instrument and PC-based software in conjunction with the specific assay. This evaluation
must include the confirmation that performance characteristics for the specific assay are
met.
• This system is designed for use with PC-based software only. BioTek’s software
packages, Gen5™ and KC4™, provide the user with instrument control.
• The Synergy HT can operate with standard robotic systems, such as BioTek’s
BioStack™ Microplate Stacker.
• The intended use of this instrument is dependent on the instrument’s labeling. If
there is an IVD label, then the instrument may be used for clinical, research and
development, or other non-clinical purposes. If there is no such label, then the
instrument may only be used for research and development, or for other non-
clinical purposes.
Quality Control
It is considered good laboratory practice to run laboratory samples according to
instructions and specific recommendations included in the assay package insert for
the test to be conducted. Failure to conduct Quality Control checks could result in
erroneous test data.
Warranty & Product Registration
Please take a moment to review the Warranty information that shipped with your
product. Please also register your product with BioTek to ensure that you receive
important information and updates about the product(s) you have purchased.
You can register online through the Customer Care Center at www.biotek.com or by
calling 888/451-5171 or 802/655-4740.

xiv | Preface
BioTek Instruments
Warnings
Operate the instrument on a flat surface away from excessive humidity.
Bright sunlight or strong incandescent light can reduce the linear performance range
of the instrument.
Measurement values may be affected by extraneous particles (such as dust) in the
microplate wells. A clean work area is necessary to ensure accurate readings.
When operated in a safe environment according to the instructions in this document,
there are no known hazards associated with the instrument. However, the operator
should be aware of certain situations that could result in serious injury; these may
vary depending on the instrument model. See Hazards and Precautions.
Hazards and Precautions
Hazards
Warning! Power Rating. The instrument’s power supply or power cord must be
connected to a power receptacle that provides voltage and current within the specified
rating for the system. Use of an incompatible power receptacle may produce electrical
shock and fire hazards.
Warning! Electrical Grounding. Never use a two-prong plug adapter to connect
primary power to the external power supply. Use of a two-prong adapter disconnects
the utility ground, creating a severe shock hazard. Always connect the power cord
directly to a three-prong receptacle with a functional ground.
Warning! Internal Voltage. Always turn off the power switch and unplug the
power supply before cleaning the outer surface of the instrument.
Warning! Liquids. Avoid spilling liquids on the instrument; fluid seepage into
internal components creates a potential shock hazard. Wipe up all spills immediately.
Do not operate the instrument if internal components have been exposed to fluid.
Warning! Potential Biohazards. Some assays or specimens may pose a biohazard.
Adequate safety precautions should be taken as outlined in the assay’s package insert.
Always wear safety glasses and appropriate protective equipment, such as chemically
resistant rubber gloves and apron.
Warning! Hot Surface. The fluorescence lamp assembly is hot when the instrument
is turned on. Turn off the reader and allow the lamp to cool down before attempting
to replace it.
Warning! Unspecified Use. Failure to operate this equipment according to the
guidelines and safeguards specified in this manual could result in a hazardous
condition.

Hazards and Precautions | xv
Synergy HT Operator’s Manual
Warning! Software Quality Control. The operator must follow the manufacturer’s
assay package insert when modifying software parameters and establishing reading
methods. Failure to conduct quality control checks could result in erroneous
test data.
Warning! Pinch Hazard. Some areas of the Dispense Module can present pinch
hazards when the instrument is operating. These areas are marked with the symbol
shown here. Keep hands/fingers clear of these areas when the instrument is
operating.
Precautions
The following precautions are provided to help avoid damage to the instrument:
Caution: Service. The Synergy™ HT should be serviced by BioTek authorized
service personnel. Only qualified technical personnel should perform troubleshooting
and service procedures on internal components.
Caution: Environmental Conditions. Do not expose the system to temperature
extremes. For proper operation, ambient temperatures should remain between
18°-40°C. Performance may be adversely affected if temperatures fluctuate above or
below this range. Storage temperature limits are broader.
Caution: Sodium Hypochlorite. Do not expose any part of the instrument to the
recommended diluted sodium hypochlorite solution (bleach) for more than 20
minutes. Prolonged contact may damage the instrument surfaces. Be certain to rinse
and thoroughly wipe all surfaces.
Caution: Power Supply. Only use the power supply shipped with the instrument.
Operate this power supply within the range of line voltages listed on it.
Caution: Shipping Panel and Carrier Shipping Screw. The shipping panel and
carrier shipping screw must be removed before operating the reader. They must be
reinstalled before repackaging the reader for shipment. See Chapter 2, Installation.
Caution: Disposal. This instrument contains printed circuit boards and wiring with
lead solder. Dispose of the instrument according to Directive 2002/96/EC, “on waste
electrical and electronic equipment (WEEE).”
Caution: Electromagnetic Environment. Per IEC 61326-2-6 it is the user’s
responsibility to ensure that a compatible electromagnetic environment for this
instrument is provided and maintained in order that the device will perform as
intended.
Caution: Electromagnetic Compatibility. Do not use this device in close
proximity to sources of strong electromagnetic radiation (e.g. unshielded intentional
RF sources), as these may interfere with the proper operation.
Caution: Warranty. Failure to follow preventive maintenance protocols may void
the warranty. See Chapter 5, Preventive Maintenance.

xvi | Preface
BioTek Instruments
Based on the testing described below and information contained
herein, this instrument bears the CE mark
Directive 2004/108/EC: Electromagnetic Compatibility
Emissions - Class A
The system has been type tested by an independent, accredited testing laboratory
and found to meet the requirements of EN 61326-1 for Radiated Emissions and
Line Conducted Emissions. Verification of compliance was conducted to the limits
and methods of the following: CISPR 16-1, CISPR 16-2, and EN 55022.
This equipment has been designed and tested to CISPR 11 Class A. In a domestic
environment it may cause radio interference, in which case, you may need to
mitigate the interference.
Immunity
The system has been type tested by an independent, accredited testing laboratory
and found to meet the requirements of EN 61326-1 for Immunity. Verification of
compliance was conducted to the limits and methods of the following:
EN 61000-4-2 Electrostatic Discharge
EN 61000-4-3 Radiated EM Fields
EN 61000-4-4 Electrical Fast Transient/Burst
EN 61000-4-5 Surge Immunity
EN 61000-4-6 Conducted Disturbances
EN 61000-4-11 Voltage Dips, Short Interruptions and Variations
Directive 73/23/EEC Low Voltage (Safety)
The system has been type tested by an independent testing laboratory and was found
to meet the requirements of EC Directive 73/23/EEC for Low Voltage. Verification of
compliance was conducted to the limits and methods of the following:
EN 61010-1. “Safety requirement for electrical equipment for measurement, control
and laboratory use. Part 1, General requirements.”
Directive 2002/96/EC: Waste Electrical and Electronic Equipment
Disposal Notice. This instrument contains printed circuit boards and wiring with
lead solder. Dispose of the instrument according to Directive 2002/96/EC, “on waste
electrical and electronic equipment (WEEE)” or local ordinances.
Directive 98/79/EC: In Vitro Diagnostics (if labeled for this use)
• Product registration with competent authorities
• Traceability to the U.S. National Institute of Standards and Technology (NIST):
Optical density measurementsare traceable to NIST.

Electromagnetic Interference and Susceptibility | xvii
Synergy HT Operator’s Manual
Electromagnetic Interference and Susceptibility
USA FCC CLASS A
Warning: Changes or modifications to this unit not expressly approved by the
manufacturer could void the user's authority to operate the equipment.
This equipment has been tested and found to comply with the limits for a Class A
digital device, pursuant to Part 15 of the FCC Rules.
These limits are designed to provide reasonable protection against harmful
interference when the equipment is operated in a commercial environment. Like all
similar equipment, this equipment generates, uses, and can radiate radio frequency
energy and, if not installed and used in accordance with the instruction manual,
may cause harmful interference to radio communications. Operation of this
equipment in a residential area is likely to cause interference, in which case the
user will be required to correct the interference at his own expense.
Canadian Department of Communications Class A
This digital apparatus does not exceed Class A limits for radio emissions from
digital apparatus set out in the Radio Interference Regulations of the Canadian
Department of Communications.
Le present appareil numerique n'met pas du bruits radioelectriques depassant les
limites applicables aux appareils numerique de la Class A prescrites dans le
Reglement sur le brouillage radioelectrique edicte par le ministere des
Communications du Canada.
User Safety
This device has been type tested by an independent laboratory and found to meet the
requirements of the following:
• Underwriters Laboratories UL 61010A-1, 1st edition, 2002
“Electrical Equipment for Laboratory Use; Part 1: General Requirements”
• Canadian Standards Association CAN/CSA C22.2 No. 1010.1-1992
“Safety requirements for electrical equipment for measurement, control and
laboratory use; part 1: general requirements”

xviii | Preface
BioTek Instruments
Safety Symbols
Some of the following symbols may appear on the instrument.
Alternating current
Courant alternatif
Wechselstrom
Corriente alterna
Corrente alternata
Direct current
Courant continu
Gleichstrom
Corriente continua
Corrente continua
Both direct and alternating current
Courant continu et courant alternatif
Gleich - und Wechselstrom
Corriente continua y corriente alterna
Corrente continua e corrente alternata
Earth ground terminal
Borne de terre
Erde (Betriebserde)
Borne de tierra
Terra (di funzionamento)
Protective conductor terminal
Borne de terre de protection
Schutzleiteranschluss
Borne de tierra de protección
Terra di protezione
On (Supply)
Marche (alimentation)
Ein (Verbindung mit dem Netz)
Conectado
Chiuso
Off (Supply)
Arrêt (alimentation)
Aus (Trennung vom Netz)
Desconectado
Aperto (sconnessione dalla rete di alimentazione)