LI-COR Odyssey M 3340 User manual

Odyssey® M
Operator’s Manual

CE Marking:
This product (model number 3340, 3350) is a CE-marked product. For conformity information,
contact LI-COR Support at http://www.licor.com/biotechsupport. Outside of the U.S., contact your
local sales office or distributor.
奥德赛M 成像仪
Odyssey M Imager
部件名称
Part Name
有毒有害物质或元素
Toxic and Hazardous Substances or Elements
铅
(PB)
汞
(Hg)
镉
(Cd)
六价铬
(Cr(VI))
多溴联苯
(PBB)
多溴二苯醚
(PBDE)
印刷电路板
(PCBs) X O O O O O
机电配件
(Electromechanical Parts) X O O O O O
光学
(Optics) X O X O O O
电缆和电线
(Cables and Wires) OOOOOO
金属部件
(Metal Parts) X O O O O O
塑料零件
(Plastic Parts) X O O O O O
电池
(Batteries) OOOOOO
本表格依据 SJ/T 11364 的规定编制
O = 表示该有害物质在该部件所有均质材料中的含量均在 GB/T 26572 规定的限量要求以下
indicates that the content of the toxic and hazardous substance in all the Homogeneous Materials of the part is below the concentration limit
requirement as described in GB/T 26572
X = 表示该有害物质至少在该部件的某一均质材料中的含量超出 GB/T 26572 规定的限量要求
indicates that the content of the toxic and hazardous substance in at least one Homogeneous Material of the part exceeds the concentration limit
requirement as described in GB/T 26572
Page 3

Notes on Safety
LI-COR products have been designed to be safe when operated in the manner described in this manual. The
safety of this product cannot be guaranteed if the product is used in any other way than is specified in this
manual. The Odyssey® M Imager is intended to be used by qualified personnel. Read this entire manual before
using the Odyssey M Imager.
Equipment Markings:
The product is marked with this symbol when it is necessary for you to refer to the
manual or accompanying documents in order to protect against damage to the product.
The product is marked with this symbol when a hazardous voltage may be present.
Manual Markings:
WARNING Warnings must be followed carefully to avoid bodily injury.
CAUTION Cautions must be observed to avoid damaging your equipment.
NOTE Notes contain additional information and useful tips.
IMPORTANT Information of importance to prevent procedural mistakes in the operation of the
equipment or related software. Failure to comply may result in a poor experimental
outcome but will not cause bodily injury or equipment damage.
Federal Communications Commission Radio Frequency Interference Statement
WARNING: This equipment generates, uses, and can radiate radio frequency energy and if not installed in
accordance with the instruction manual, may cause interference to radio communications. It has been tested
and found to comply with the limits for a Class A computing device pursuant to Subpart J of Part 15 of FCC
rules, which are designed to provide a reasonable protection against such interference when operated in a
commercial environment. Operation of this equipment in a residential area is likely to cause interference in
which case the user, at his own expense, will be required to take whatever measures may be required to
correct the interference.
Page 4

Notice
The information contained in this document is subject to change without notice. LI-COR MAKES NO
WARRANTY OF ANY KIND WITH REGARD TO THIS MATERIAL, INCLUDING, BUT NOT LIMITED TO
THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE.
LI-COR shall not be liable for errors contained herein or for incidental or consequential damages in connection
with the furnishing, performance, or use of this material.
This document contains proprietary information that is protected by copyright. All rights are reserved. No part
of this document may be photocopied, reproduced, or translated to another language without prior written
consent of LI-COR, Inc.
Printing History
Publication Number 977-19674
Published: June 15, 2021
Revised: June 15, 2021
Global Headquarters
4647 Superior Street • Lincoln, NE 68504
Toll free: 800-645-4267 • Fax: [phone] • [email protected] • licor.com/bio
Regional Offices
LI-COR Biosciences GmbH
Siemensstraße 25A • 61352 Bad Homburg • Germany
Phone: [phone] • Fax: [phone] • [email protected]
LI-COR Biosciences UK Ltd.
St. John’s Innovation Centre • Cowley Road
Cambridge • CB4 0WS
United Kingdom • Phone: [phone] • Fax: [phone] • [email protected]
LI-COR Biotechnology Distributor Network: www.licor.com/bio/distributors
LI-COR is an ISO 9001:2015 registered company. © 2021 LI-COR Inc. All rights reserved. Specifications subject
to change. LI-COR, Odyssey, and In-Cell Western are trademarks or registered trademarks of LI-COR, Inc. in the
United States and other countries. All other trademarks belong to their respective owners.
Page 5

Chapter 1: Installation and Safety Information
Safety Considerations 9
Intended Use of the Odyssey M Imager 9
Odyssey M Model Numbers (3340 and 3350) 9
Laser Safety 9
Safety Interlocks 12
Placement in the Laboratory 12
Ambient Laboratory Conditions 12
Imager Ventilation 13
Space Requirements 13
Moving the Imager 13
Leveling the Imager 14
Chemical Safety 15
Electrical Considerations 15
Power Cords 15
Fuse Information 16
Routine Maintenance 16
Inspect Cables and Cords 16
Maintain Scan Surface 16
Maintain Imager Exterior 16
Clean or Replace Fan Filter 17
Networking Cables 17
Chapter 2: System Overview
External Panels and Controls 19
Front Panel and Scan Surface 19
Rear Panel 32
General Description of Scanning 35
LI-COR Acquisition Software Overview 35
Continuous Operation 35
Disconnecting the Power 35
Optical System Description 35
Brief Description of Illumination Sources 35
Computer Connections and Networking 37
Odyssey® M
Imager
Table of Contents
Page 6

Chapter 3: Operation
Before You Begin 39
Clean the Scan Surface 39
Clean Silicone Mat (926-70003) 40
Clean Alignment Guides (926-18972 and 926-18971) 40
Imaging Membranes 40
Handling Membranes 40
Scanning Fluorescent and Colorimetric Membranes 41
Scanning Chemiluminescent Membranes 41
Imaging Gels 42
Scanning Gels 42
Imaging Multiwell Plates 42
Scanning Multiwell Plates 43
Imaging Slides 43
Scanning Slides 43
Obtaining Technical Support 45
Chapter 4: Appendix
Imaging System Specifications 47
Operating Specifications 48
Odyssey M Detection Channel Summary 50
Embedded Firmware Source Code 52
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Chapter 1: Installation and Safety Information
Safety Considerations
Intended Use of the Odyssey M Imager
The Odyssey M provides reliable data for membranes, plate-based assays, slides, nucleic acid gels, and
protein gels. The Odyssey M is versatile and can capture assays in 18 channels, including white light,
luminescence (bioluminescence and chemiluminescence), and fluorescence (near-infrared / visible).
Odyssey M Model Numbers (3340 and 3350)
Odyssey M Model 3350 and Odyssey M Model 3340 are the same, except that Odyssey M Model 3350
contains hardware that allows it to image chemiluminescence. See "Imaging System Specifications"
on page47 for details.
Laser Safety
The Center for Devices and Radiological Health (CDRH) was established in October 1982, by the
U.S. Food and Drug Administration (FDA) to protect the public health in the fields of medical
devices and radiological health.
Manufacturers of products subject to performance standards under the Radiation Control for Health
and Safety Act of 1968 are required to furnish various reports to the CDRH.
The Odyssey M is certified as a Class I laser product. This means that hazardous laser radiation is not
emitted outside the instrument. Radiation emitted inside the Odyssey M is confined within protective
housings and external covers. A series of safety interlocks ensures that the laser beam cannot escape
during any phase of user operation.
The CDRH implemented regulations for laser products on August 1, 1976 (CDRH radiation
performance standard 21, Code of Federal Regulations Chapter 1, Subchapter J). Compliance for
products marketed in the United States is mandatory. The label that must be attached to laser
products marketed in the United States is illustrated in Figure 1-2 and is located on the rear panel of
the Odyssey® M, indicating compliance with CDRH regulations.
WARNING: Use of controls or adjustments or performance of procedures other than those specified
herein may result in hazardous radiation exposure.
AVERTISSEMENT: L'utilisation des commandes ou le réglage ou la performance des procédures
autrement qu'il a été indiqué dams le présent texte peut résulter en de dangereuses expositions aux
radiations.
The manufacturer label is illustrated in Figure 1-2 and is also on the rear panel of the instrument.
Odyssey® M
Imager
Page 9

Chapter 1: Installation and Safety Information
The Odyssey® M Near-Infrared Imaging System contains four lasers; one emitting at 488 nm, 520
nm, 685 nm, and 785 nm. The lasers emit visible and invisible laser radiation - avoid eye or skin
exposure to direct or scattered radiation. Laser radiation is emitted out of the top of the Odyssey
M microscope, up through the scan glass. Because the microscope moves in both planes relative to
the glass scanning surface, laser radiation could be focused at any position on this scanning surface.
Safety interlocks (described below) automatically turn the lasers off when the Odyssey M lids are
opened.
Labels
The labels shown in Figure 1-1 and Figure 1-2 are affixed to the Odyssey M case.
P/N: 250-19148
DOC#: 13-19454
Figure 1-1. Laser radiation danger label. Figure 1-2. Label with CDRH compliance statement.
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Odyssey® M Imager
Imager Label Locations
Figure 1-3. The label affixed to the rear panel of the imager contains ETL, CDRH regulation compliance, and
manufacturer information.
Figure 1-4. A laser safety label is visible on the front panel of the imager with the Tray Holder removed.
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Chapter 1: Installation and Safety Information
Figure 1-5. There is a laser safety label on the bottom panel of the imager.
Safety Interlocks
The Odyssey® M Imager has safety interlocks that prevent access to the lasers, LEDs, and moving
parts, by disabling these hazards when the front or rear lid is opened.
Do not attempt to defeat these interlocks.
Placement in the Laboratory
The Odyssey M Imager Model 3340 weighs 115 lbs (52kg). The Odyssey M Imager Model 3350
weighs 121 lbs (55kg). The imager should be placed on a laboratory bench that is sufficiently sturdy
to bear its weight. The bench should also be able to bear the weight of the computer if the computer
will be installed beside the Odyssey M Imager.
The Odyssey M is intended for indoor use only.
Ambient Laboratory Conditions
Place the Odyssey M Imager away from external heat sources (furnaces, windows, etc.). Additional
heating can cause high temperatures within the enclosure. Place the instrument away from sinks or
other sources of water that pose a shock hazard. Recommended operating conditions are 15 - 35 °C
and a dew point not greater than 22 °C to prevent condensation on the laser/microscope assembly
during operation. Allow the instrument to adjust to the temperature and humidity of the room
before powering it on to avoid damaging the instrument. It may be necessary to allow up to a day in
the new location in cases of extreme temperature and/or humidity change.
Page 12

Odyssey® M Imager
Imager Ventilation
The instrument enclosure and circuit boards are cooled with multiple internal fans. The fan on the
rear of the instrument serves as an exhaust port, so the fan shrouds are not filtered, and the cover
serves only as an exhaust outlet. There are no restrictions regarding placement of the instrument as
spacers on the rear of the instrument ensure sufficient airflow for the exhaust port. However, it is
important to not allow any objects (e.g., paper or a cover) to block airflow from the exhaust port
while the instrument is on.
Space Requirements
The Odyssey M Imager requires an area approximately 24" width x 30" depth x 15" height (61 cm
width x 76 cm depth x 38 cm height). With the lid fully open, the Odyssey M has a height of 28" (71
cm).
Moving the Imager
LI-COR recommends that you do not move your imager. Instead, please contact LI-COR Technical
Support for options.
LI-COR cannot be held responsible for any damage incurred while moving, transporting, or
shipping the Odyssey M Imager, even if an existing warranty and/or service contract applies.
LI-COR cannot be held responsible for any injury incurred while moving, transporting, or
shipping the Odyssey M Imager.
Page 13

Chapter 1: Installation and Safety Information
Leveling the Imager
Leveling the imager is important for many reasons, including to prevent liquid from pooling in the
imaging tray and to keep the imager from tilting.
The imager sits on three feet. The two front feet can be used to raise or lower the front corners of the
imager. The back foot cannot be adjusted.
Feet
Figure 1-6
Follow these steps to level the imager:
1. Place a circular bubble level on the center of the Scan Surface.
Figure 1-7
Use the bubble level to determine if the imager is level or which adjustments might be needed to
level the imager.
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Odyssey® M Imager
2. Slightly raise the front of the imager to remove pressure from the foot you want to adjust.
3. Turn the screw on the foot clockwise to lower the corner or counterclockwise to raise the corner.
Figure 1-8 Turn the metal inserts on the feet to adjust the height.
Chemical Safety
LI-COR Biosciences recommends that all biochemicals be handled carefully, and that safe laboratory
procedures be followed at all times. Be aware of the hazards associated with any chemical before you
begin work.
The LI-COR Odyssey M should not be used with any radioactive materials.
Electrical Considerations
Power Cords
The Odyssey M is provided with a power supply that converts wall voltage to 24 V DC. Use only the
provided power supply cord to connect the Odyssey M to the wall outlet.
lThe plug will only fit into a grounding-type outlet. This is a necessary safety feature. If you are
unable to insert the plug into the outlet, you will need to replace the outlet. Do not defeat the
purpose of the grounding-type plug.
lDo not place the Odyssey M where the power cord will be walked on or exposed to water or
chemical spills.
lThe Odyssey M draws < 3 Amps at 120V. If an extension cord is used, make sure the total of the
ampere ratings on the instruments plugged into the extension cord does not exceed the extension
cord ampere rating. Also make sure the total amperage of instruments plugged into the wall
outlet does not exceed the amperage capacity for the outlet (usually 15 or 20 amperes in the
United States).
lIf the power supply's DIN8 output connector (Figure 1-9) is handled improperly (such as if the
pins are pressed against a screw on the imager chassis), the pins in the power supply's DIN8
output connector can short or spark.
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Chapter 1: Installation and Safety Information
Figure 1-9
lFor information about disconnecting power, see Disconnecting the Power on page 35.
Fuse Information
There are no user-serviceable fuses. If the imager does not power on, please contact LI-COR
Biosciences or a LI-COR representative.
Routine Maintenance
The Odyssey® M requires only minimal maintenance. However, as with any equipment utilizing
electrical voltages, there is a danger of fire or electrical shock if the equipment is not properly
maintained.
LI-COR Biosciences recommends that you routinely inspect the system and the Scan Surface. The
following are some general maintenance guidelines.
Inspect Cables and Cords
Inspect all cables and power cords for evidence of fraying, exposed wire, or loose connections.
Maintain Scan Surface
lRemove all chemical spills from the Scan Surface to prevent damage to the surface coating.
lClean the Scan Surface according to instructions in "Clean the Scan Surface" on page39.
lPrevent organic solvents from pooling on the imager's Scan Surface and keep the Scan Surface
from contacting other combustibles.
Maintain Imager Exterior
lRemove all chemical spills from the imager exterior to prevent damage to the imager exterior.
lClean the exterior case parts with warm water and a damp cloth.
Do not use scouring compounds or solvents (e.g., acetone, benzene, carbon tetrachlorides,
lacquer thinner, or alcohol) to clean the case.
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Odyssey® M Imager
Clean or Replace Fan Filter
The fan filter behind the Tray Holder should be inspected and cleaned at least once a year, but more
frequent cleaning may be necessary depending on the cleanliness of the imager's surroundings.
If the fan filter becomes damaged, contact LI-COR for a replacement.
Follow this procedure when you need to inspect or clean the fan filter:
1. Open and remove the Tray Holder (see Remove Tray Holder on page 28).
2. Remove the screw holding the retaining plate in place using a No. 2 screwdriver.
Figure 1-10Exploded view of the fan filter assembly
3. Remove the retaining plate and the fan filter.
4. To clean the fan filter, flush it with water. If flushing the filter with water does not adequately
remove oil and trapped particles, you can immerse the filter in hot water and detergent.
5. When the fan filter is clean, you can wring it out to remove most of the water. Wait for the fan
filter to dry completely.
6. Once the fan filter is completely dry, place the fan filter, retaining plate, and Tray Holder back on
the imager (see Install Tray Holder on page 30).
Networking Cables
Figure 1-11. Networking
port label.
A Category 6 network cable is provided with the Odyssey M Imager. The
networking port is on the back panel of the imager and is marked with the
symbol in Figure 1-11.
Page 17

Page 18

Chapter 2: System Overview
External Panels and Controls
Front Panel and Scan Surface
Figure 2-1. Front panel features and power button on the Odyssey M.
Power Button
Briefly press the Power button  to turn the imager on and off when the imager is not connected to
LI-COR® Acquisition Software.
lWhen the imager is powered on, the power button will be illuminated solid blue.
lDuring the process of powering on or powering off, the power button will flash blue.
Display Panel
The front display panel shows the following:
Odyssey® M
Imager
Page 19

Chapter 2: System Overview
lThe username chosen in LI-COR® Acquisition Software when the Odyssey® M Imager was con-
nected to LI-COR Acquisition Software.
lIf the lid is open.
lThe time remaining in a scan.
lDuring a scan, the type of scan will be shown.
Front Panel Indicator Light
An indicator light is located on the front panel. In most circumstances, the color of the light mirrors
a status indicated in LI-COR Acquisition Software.
Indicator
Light
Figure 2-2
Indicator Light State Meaning
Off No connection to LI-COR Acquisition Software
Green Ready or Done
Yellow/Orange Scanning or Lid Open
Red And error has occurred. Check LI-COR Acquisition Software for more information.
Scan Surface
Gently lift the handle on the front edge of the lid to access the Scan Surface. Instructions for
acquiring images are provided in "Operation" on page39.
Gently press down on the lid when you are ready to close it. There will be slight upward resistance
when the lid is nearly in the close position to help prevent the lid from closing with excessive force.
During imaging, the Scan Surface will automatically move to the rear portion of the imager to place
the samples in the appropriate location relative to components of the optical system. When the scan
is complete, the Scan Surface will return to the front of the imager.
Page 20