Agilent Technologies G1978B User manual

Agilent Technologies
Agilent G1978B
Multimode Source for
6410 Triple Quad LC/MS
User Guide

2 Multimode Source for 6410 Triple Quad LC/MS User Guide
Notices
© Agilent Technologies, Inc. 2006
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Manual Part Number
G1978-90010
Edition
First Edition, October 2006
Printed in USA
Agilent Technologies, Inc.
5301 Stevens Creek Blvd.
Santa Clara, CA 95051 USA
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Multimode Source for 6410 Triple Quad LC/MS User Guide 3
In This Guide
This guide explains how to install, maintain and troubleshoot your multimode
ion source.
1Basic Operation and Maintenance
This chapter describes basic operation and maintenance for the multimode
source.
2Installation
This chapter tells you how to install the multimode source.
3Reference
This chapter contains an overview of the multimode source, safety
precautions, and technical specifications.

4 Multimode Source for 6410 Triple Quad LC/MS User Guide

Contents
Multimode Source for 6410 Triple Quad LC/MS User Guide 5
Content
1 Basic Operation and Maintenance 7
To set up a method to use the multimode source 8
To open the multimode source 10
To clean the multimode source daily 11
To clean the multimode source weekly 13
To check tuning with the multimode source 15
2 Installation 17
Step 1. Prepare to install 18
Step 2. Install the HV control PCA and cables 19
To remove the multimode source 23
To convert from multimode to ESI or APCI 24
To convert from ESI or APCI to the multimode source 25
3 Reference 31
Safety and Specifications 32
Safety 32
Safety Symbols 36
Technical Specifications 37
Overview 38
Benefits of a multimode source 38
Mobile phase considerations when using the multimode source 46
Source comparison in positive ion mode 48
Source comparison in negative ion mode 49
Guidelines 50
Guidelines for multimode source settings in MM-ES mode 50
Guidelines for multimode source settings in MM-APCI mode 51
Guidelines for multimode source settings in MM-ES+APCI mode 52
Empirical Formulas for high resolution MS 53

6 Multimode Source for 6410 Triple Quad LC/MS User Guide
Contents
G2421A MM-ES Calibration Ions 53
G2432A MM-APCI Calibration Ions 54
Tuning Mix and Test Mix 55
G2421A ES Tuning Mix (100mL) 55
G2432A APCI Tuning Mix (100mL) 56
Chemical structures in the multimode test mix 57

7
Agilent G1978B Multimode Source for 6410 Triple Quad LC/MS
User Guide
Agilent Technologies
1
Basic Operation and Maintenance
To set up a method to use the multimode source 8
To open the multimode source 10
To clean the multimode source daily 11
To clean the multimode source weekly 13
To check tuning with the multimode source 15
This chapter describes the tasks that you need to operate and maintain the
multimode source.
s

8 Multimode Source for 6410 Triple Quad LC/MS User Guide
1 Basic Operation and Maintenance
To set up a method to use the multimode source
To set up a method to use the multimode source
1In the MassHunter software, change the Context to Acquisition.
2In the MS QQQ tab, set Ion source to MMI (see Figure 1 on page 9).
3In the Time Segments table, chose an ionization mode from the Ion Mode
list. You may set the ionization mode to one of the following:
•ESI
•APCI
•ESI+APCI
The Ion Mode selection ESI + APCI will specify a method for alternating ESI
and APCI operation.
Note that the Ion Mode selection is only visible if Ion source is set to MMI.
4In the Source tab, set the desired source conditions. See the
“Guidelines” on page 50 for suggested source conditions for the multimode
source for the different ionization modes.
5Make any other changes that are necessary for your method.
6Save the method.

Multimode Source for 6410 Triple Quad LC/MS User Guide 9
Basic Operation and Maintenance 1
To set up a method to use the multimode source
Figure 1 Multimode acquisition settings
WARNING The LC/MS diverter valve is an integral part of the G1978B safety system. The LC
mobile phase flow must always be connected to the diverter valve inlet filter. Never
bypass the diverter valve and connect directly to the nebulizer. If the diverter valve is
used in a manner not specified by Agilent Technologies, the protections provided by
the diverter valve may be impaired and the system may catch fire.

10 Multimode Source for 6410 Triple Quad LC/MS User Guide
1 Basic Operation and Maintenance
To open the multimode source
To open the multimode source
Open the multimode source to access the end cap and the capillary cap for
cleaning and inspection.
1Turn off the multimode source temperatures and flows:
aChange the Context view to Acquisition.
bClick the MS QQQ tab.
cTurn off all voltages and temperatures in the Source tab.
dWait approximately 20 minutes for the source to cool down.
2Open the spray chamber cover by pulling the latch.
The high voltage automatically turns off when the chamber door is opened
so that no high voltages are present within the chamber.
3Check that the vaporizer temperature sensor is straight and extends 15mm
from back of chamber.
4Check that the separator is aligned vertically.
5Check that the APCI corona needle is in and extends approximately 3mm
from the corona guide.
6Check that the source is clean.
WARNING Do not touch the multimode source or the capillary cap. They may be very hot. Let the
parts cool before you handle them.
WARNING Never touch the source surfaces, especially when you analyze toxic substances or
when you use toxic solvents. The source has several sharp pieces which can pierce
your skin including the APCI corona needle, vaporizer sensor and counter current
electrode.
WARNING Do not insert fingers or tools through the openings on the multimode chamber. When
in use, the capillary and capillary cap are at high voltages up to 4 kV.