BiOptic Qsep1 User manual

BiOptic Inc.,
4F., No.108-3, Minquan Rd., Xindian Dist., New Taipei City 23141, Taiwan (R.O.C.)
www.bioptic.com.tw
Revolution
Qsep1& Qsep100 Operation Manual
--- Hardware

2
Welcome
Thank you for purchasing Qsep™ Series single channel capillary electrophoresis
system. Now you can experience this high-performance, fully-automated and easy-
to-use system.
Copyright and Trademarks
Copyright © BiOptic Inc.
All rights reserved. Reproduction, adaptation, or translation of this manual is
prohibited without prior written permission of BiOptic Inc., except as allowed under
the copyright laws.
Qsep™ Series and Q-Analyzer™ are registered trademarks of BiOptic Inc.
Microsoft Windows is registered trademarks of Microsoft Corporation.
Product and company names listed are trademarks or trade names of their
respective companies.
Symbols of Qsep Series
Symbol
Description
CE mark for European Conformity.
FCC mark of the United States Federal Communications
Commission.
*FCC ID for RFID Module: 2AUQB

3
Welcome........................................................................................................................2
Copyright and Trademarks........................................................................................2
Symbols of Qsep Series................................................................................................2
Applications..................................................................................................................4
Packing List..................................................................................................................4
Cautions........................................................................................................................5
1. System Overview......................................................................................................7
1.1 Qsep100™Instrument............................................................................................8
1.1.1 Qsep100™ Front View.....................................................................................8
1.1.2 Qsep100™ Back View....................................................................................10
1.1.3 Qsep100™ Top View......................................................................................11
1.2 Qsep1™ Instrument............................................................................................12
1.2.1 Qsep1™ Front View.....................................................................................12
1.2.2 Qsep1™ Back View......................................................................................13
1.2.3 Qsep1™ Top View........................................................................................14
1.3 Hardware Requirements.................................................................................15
2. Installation Instruction..........................................................................................16
2.1 Environment Requirements............................................................................16
2.2 Power Considerations......................................................................................17
2.3 Hardware Installation .....................................................................................18
2.3.1 Qsep100™Installation...................................................................................18
2.3.2 Qsep1™ Installation ....................................................................................19
3. Quick Start .............................................................................................................20
3.1 Qsep1™ Quick Start ...........................................................................................20
3.2 Qsep100™ Quick Start.........................................................................................28

4
Limitation of Liability
Qsep™ Series Bio-Fragment Analyzer, Q-Analyzer™software, and all related
reagents are designed for the use of electrophoresis analysis in general bio-
chemistry laboratory. If you use it for profit, diagnosis, or other non-research
purposes experiments, the company is not responsible for the accuracy and
reliability of the result and the safety of operation.
BEFORE ATTEMPTING TO OPERATE THE INSTRUMENT, READ ALL PRODUCT
MANUALS AND FOLLOW THE INSTRUCTIONS.
BiOptic Inc. assumes no liability whatsoever for any personal injury, property
damage, or other loss resulting from not complying or familiar with the manuals, or
improper operation of the devices.
Applications
Genetic Profile Screening
Amplified Fragment Length Polymorphisms
Bacterial/Viral Genotyping and Identification
DNA Fragment/PCR Product Analysis
Rapid Analysis of Genomic DNA Samples
RNA Analysis
Protein Analysis
Packing List
Each Qsep™ Series Instrument package comes with the following:
Qsep™ Series CE Instrument
Qsep™ Series Standard and High resolution cartridge Kit (C105200)
Power Cord with Adapter
USB Cable
Software Key (Basic edition)
Installation Disc including Q-Analyzer™and Qsep™ Series Operation Manual
*Note: The Software key contains 8GB storage space, Q-Analyzer™and Qsep™
Series Operation Manual, including Hardware version and Software version.

5
Cautions
Qsep™ Series are capillary electrophoresis instruments driven by high voltage.
Carefully read and thoroughly comply with the following instructions to maintain the
integrity of the equipment, the reliability of results, and the safety of operation.
Keep Qsep™ Series away from other electronic device and voltage sources.
1. Only the components and consumables provided by BiOptic Inc. are
suggested to use.
2. DO NOT perform the following actions:
•Disassemble Qsep™ Series instrument and its associated parts
•Bumps or jolts to Qsep™ Series
•Move the instrument or remove any connected external equipment,
such as computers and air pumps, while Qsep™ Series are in action
•Open the sample door or cartridge door while Qsep™ Series are in
action
•Remove the cartridge while Qsep™ Series are in action
•Power off Qsep™ Series before confirming Qsep™ Series are inactived
3. Store the cartridge upright; do not lay the cartridge on a table horizontally
or place it upside down.
4. Store the cartridge in the calm-shell container (Cartridge needs to be
stored in a proper environment as the instructions of cartridge unpacking
guide to maintain its lifetime and quality).
5. Ensure the cartridge is taking out from Qsep100™ before shutting down or
disconnecting instrument. Or ensure the cartridge is placed at PARK
position before shutting down or disconnecting Qsep™ Series. Failure to do
so will cause dryness of the gel at the cartridge tip, which will damage the
cartridge.
6. Before operating Qsep™ Series, ensure the buffer tray is placed properly.
7. Dilute the PCR DNA test sample to 1
20 with our dilution buffer before
running the test for obtaining better result and retaining the lifetime of the
cartridge. You can adjust the dilution ratio after primary test runs.
8. Ensure that the test sample, alignment marker, cartridge, and buffer tray
have been removed from the instrument before transporting or shipping.
9. If the buffer solution spills out on the platform inside the instrument, wipe
it with tissue and clean it up immediately.
10. Ensure that the main power of the instrument has been shut down and all

6
the test materials have been removed before cleaning Qsep™ Series .
11. User can also use N2 gas as an air source to insure the air quality and
prevent damages to the devices caused by dirty air.
12. Make sure to press the plastic connection ring before pulling the air tube
out from the back of the instrument.
13. Release the condensation water in the external air pump periodically and
check water level before each use to avoid the mist damage the devices of
Qsep™ Series.
14. To cut off the power please disconnect the power plug.
15. Please clean the instrument with dry cloth. For other maintenance, please
contact local distributor.
16. Please use the MAINS power cord within adequate rating.
17. The conditions of transportation and storage: Temperature -30 to +60°C
Humidity 20 to 80% RH, non-condensing.
*Note: Strongly suggest performing this step whenever you start to operate
Qsep™Series .
Warning: Qsep™Series are high-voltage Single-channel electrophoresis
system. Please follow the operation manual and laboratory safety guidelines for
system operation. Do not remove covers. For operation and safety questions,
please contact BiOptic Inc. at the official website or with your local BiOptic
representatives.

7
1.System Overview
Qsep™ Series are a fully-automated CE system developed by BiOptic Inc., which uses
pen-shaped disposable gel-cartridges to improve efficiency. Time-consuming manual
procedures such as gel preparation, sample loading, and capillary changing are no
longer required. Further, the information will be obtained easily with the fully-
automated Qsep™ Series. Qsep100™and Qsep1™are designed to accommodate the
standard 96-well and 8-12 well PCR plate, respectively.
Qsep™ Series’s compact design helps you to set up and operate the instrument
intuitively. The disposable gel-cartridge with integrated pre-programmed test
methods makes capillary electrophoresis experiments no longer a painful procedure
that requires well-trained operators. No more worries about operation and human
errors caused by different operators which affect the accuracy and reproducibility of
the results.
The following sections will describe the product overview, the functions of Qsep™
Series instruments and the gel-cartridge. Please read through this section and get
ready to be amazed with the power of Qsep™ Series.
Caution: The operator of this instrument is advised that if the equipment is
operated in a manner not specified in this manual, the protection provided by the
equipment may be impaired.

8
1.1 Qsep100™Instrument
1.1.1 Qsep100™ Front View
Figure 1-1 Qsep100™front view
A. High voltage alert lamp:
The red LED blinks when the high voltage source of the capillary electrophoresis
is ON.
B. Main Power switch:
The blue LED lights up when it is turned on.
C. Operation room:
Open the sample door. A rotation platform includes three regions for operation
which are sample array region (i), marker array region (ii), and buffer solution
region (iii). (Figure 1-2)

9
Figure 1-2 Rotation platform

10
1.1.2 Qsep100™ Back View
Figure 1-3 Qsep100™back view
A. Air pressure gauge:
Display the value of the pressure operating in Qsep100™.
B. Air flow Inlet Port:
Connect to an external pump using an 1/8 inches tube.
C. USB Port:
Connect to a computer.
D. Power Connector
E. Qsep100™Serial Number

11
1.1.3 Qsep100™ Top View
Figure 1-4 Qsep100™top view
A. Cartridge door

12
1.2 Qsep1™ Instrument
1.2.1 Qsep1™Front View
A. Indicator lamp:
B. SD Card Port
C. Cartridge Door
D. Sample Door and Sample Tray

13
1.2.2 Qsep1™ Back View
A. Power Connector
B. Main Power switch
C. Air flow Inlet Port:
Connect to an external pump using an 1/8 inch tube.
D. Cable Port

14
1.2.3 Qsep1™ Top View
A. Cartridge door
A

15
1.3 Hardware Requirements
Minimum
Recommended
CPU
Intel Core i3 530
2933MHz (Dual Core)
Intel Core i7
2.8 GHz
RAM
4G RAM or More
8G RAM or More
HDD
320GB or More
500GB or More
PORT
USB 2.0 x 1
USB 3.0 x 1
INTERNET
Required

16
2. Installation Instruction
A suitable operating environment is essential to ensuring the best performance of
Qsep™ Series.
2.1 Environment Requirements
⚫The optimal operating temperature of Qsep™ Series are 18°C~26°C (65°F ~78°F).
⚫The optimal operating humidity of Qsep™ Series are 40%~95% RH, non-
condensing.
⚫The operating altitude of Qsep™ Series are -20m~2000m (-65ft~6500ft).
For proper capillary electrophoresis operation, do not place Qsep™ Series on a
vibrating surface or near vibrating objects. For convenient access for loading and
installation, it is recommended to leave approximately 711 mm (28 inches) clear
space above the counter space or table. Allow approximately 76 mm (3 inches) of
clear space at the rear and to the side of the instrument for proper air ventilation
and electrical connections.
Warning: Do not store, transport, or use Qsep™ Series under conditions which
the temperature fluctuation could cause water condensation inside the instrument.
Condensation may damage electronic devices of the instrument. If the instrument
was transported in cold weather, do not remove the instrument from its original box
immediately because it might cause condensation. Make sure Qsep™ Series have
been warmed up inside the original box before you start unpacking.

17
2.2 Power Considerations
The input voltage of Qsep100™ instrument is 24V DC, and the maximum current is
1.25A. A suitable power adapter with cord, SINPRO MPU32B-108, is included in the
package (100~240V AC, 47~63Hz). The dimensions are 38cm(L)*30cm(W)*40cm(H)
and weight of the instrument is 15Kg. Direct control the instrument with USB cable.
Connect the air source within the range of 50psi~75psi.
The input voltage of Qsep1™instrument is 12V DC, and the maximum power is
80W. A suitable power adapter with cord, MEAN WELL GSM90B12-P1M, is included
in the package (100~240V AC, 50~60Hz). The dimensions are
24cm(L)*21cm(W)*30cm(H) and weight of the instrument is 5.5Kg. Remote control
the instrument with WiFi. Connect the air source within the range of 50psi~65psi.
*Note: Use a surge protector outlet for both Qsep series and the computer to avoid
electromagnetic interferences and damages to the inner electrical devices.

18
2.3 Hardware Installation
Place Qsep™ Series instrument on a stable platform, and ensure it is away from
water and any other high-power electronic devices. Qsep™ Series need to be
operated in a clean and well-ventilated environment. Remove the power adapter
from the package and attach it with a suitable power cord.
When using the portable air pump as the air source, please pay attention to the
condensation water that might develop inside the portable air pump. If this occurs,
be sure to release the condensation water before using Qsep™ Series. If you need to
continuously operate Qsep™ Series, check the air pump every 12 hours to avoid the
mist of condensation water damaging the devices of Qsep™ Series.
2.3.1 Qsep100™Installation
Prepare a computer with Windows operation system (i.e. Windows 10). Use the
supplied USB cable to connect Qsep100™with the computer. Turn ON the power of
the computer and Qsep100™and install the operation software, Q-Analyzer™.
After checking all connections, you are now ready to start using Qsep100™.
Figure 2-1 Qsep100TM Hardware installation

19
2.3.2 Qsep1™ Installation
Prepare a computer with Windows operation system (i.e. Windows 10).
For Qsep1™,user could use the cable or WiFi function to connect the instrument
with your computer after Turn on Qsep1™.
Turn on the power, wait 25 second for WiFi function initialization (Green light
flashing) and install the operation software, Q-Analyzer™. After checking all
connections, you are ready to start using Qsep1Series.
Figure 2-2 Qsep1TM Hardware installation

20
3. Quick Start
3.1 Qsep1™ Quick Start
1. Ensure the SD card is properly plugged in the socket (Fig. 1 and 2).
SD card needs to be plugged-in before power on. The SD card CAN NOT be
initialized with hot plugging.
2. Connect the air tube.
3. Plug the power cord (labeled Qsep1)
4. Switch on Qsep1, the LED lights (multiple color light) (Fig. 3). Wait 25 seconds
for the instrument and Wi-Fi initialization, and the green LED will start flashing
(Fig. 4).
DO NOT switch on the instrument immediately after powering off. Wait at least
5 seconds
The green flashing LED shows the instrument is successfully ready for
connection. If not, please repeat the step 4.