BiOptic Qsep 1 User manual

Operation Quick Start
Bio-Fragment Analyzer: Qsep1Document No.: F0016
Ver.: C-20190726
Page: 1/3
1. Ensure the SD card is properly plugged in the
socket (Fig. 1 and 2).
3. Plug the power cord (labeled Qsep1)
*SD card needs to be plugged-in before power on.
The SD card CAN NOT be initialized with hot plugging.
4. Switch on Qsep1, the LED lights (multiple color light)
(Fig. 3). Wait 25 seconds for the instrument and Wi-
Fi initialization, and the green LED will start flashing
(Fig. 4).
-Check if the AP source is available from your Wi-Fi
network in the computer (Fig. 5).
-Find the SSID named with “Qsep1+instrument ID
(16 digits) ” and click “Connect” (Fig. 6)
-Enter the password:
The password will show in the sticker which is
behind the instrument. It is the same as the middle
8 digits of instrument serial number (same as SSID
name) by blinding the prefixal “Qsep1” (Fig. 7).
2. Connect the air tube.
Fig. 2
Fig. 1
*The green flashing LED shows the instrument is
successfully ready for connection. If not, please
repeat the step 4.
Wi-Fi connection: follow the steps below to
connect with Qsep1
Fig. 5
*DO NOT switch on the instrument immediately
after powering off. Wait at least 5 seconds
Ethernet cable connection:
refer to step 5 on Page 2
Ethernet cable
Fig. 3 Fig. 4
Fig. 6
*If your computer can’t find the SSID of Qsep1,
please keep the distance between Qsep1and the
computer within 10 meterS and try again from the
step 4.
*If you can not connect with Qsep1
-Please check the wi-fi IP assignment is “Automatic
(DHCP)”
-Allow Q-Analyzer pass through firewall or turn off
the firewall
blue
green
00923718
Fig. 7

8-well sample tray 12-well sample tray
Operation Quick Start
6. Double click the Q-Analyzer.
7. Q-Analyzer user interface:
Cartridges
Alignment maker
Separation buffer
Dilution buffer
Mineral oil
Buffer tray
Dropper
Tubes for Alignment Marker
Bio-Fragment Analyzer: Qsep1Document No.: F0016
Ver.: C-20190726
Page: 2/3
12. Open the cartridge door by pressing the white button
and insert cartridge (guiding groove facing front).
13. Close the cartridge door and click “Latch”.
Packing List of Cartridge Kit (Cat. C105200)
5. Check the LED status.
7-2. Click “Connect”, the image will switch to Qsep1
when it’s connected
8. Prepare buffer and alignment marker in 0.2ml PCR tubes and
allocate at the corresponding position on the sample tray
*For 12-well sample tray, user needs to use
0.1ml tube (C104252) for sample and size marker
*S/P/C well can only place 0.2ml PCR tubes
9. Click “Change Sample” or “Change
Buffer”, the sample door will open
automatically.
10. Slide the buffer tray into the
instrument. Make sure sample tray
is pushed to the end
11. Click “Park”, the sample tray move into instrument
*Please follow cartridge unpacking guide to unpack the
cartridge before using
Guiding groove
*The cartridge information will be displayed after latched.
*Make sure no air bubble appears in each tube
blue
green
7-1. Select “Network” mode and click “Search”
*After “Search” the instrument ID will display
*Please confirm the instrument ID is the connected instrument

Operation Quick Start
15-3. Click on “Result Name ” and enter the name of result files.
14. Cartridge Calibration:
New cartridge needS to be calibrated before using.
Please proceed with the following steps.
14-1. Click “HV Check”.
14-2. Click “Calibrate”.
* The storage and
transportation condition may
influence the Gel-matrix and
cause unstable current. During
HV Check, check current (gray
line) and see if it’s stable. if
current is unstable, please
repeat this step 2-3 times.
* For troubleshooting, please refer to cartridge unpacking
guide for details.
Bio-Fragment Analyzer: Qsep1Document No.: F0016
Ver.: C-20190726
Page: 3/3
*The “Calibrate” button will only
display after HV check passed.
15. To analyze samples, click on the blank column and designate
the sample locations, test method, runs and result name
followed steps 15-1 to 15-4
15-1. Click the “Sample Position”, mark the position of sample on
the plate and then click “OK”.
15-2. Click on “Method” to select a proper method for your application.
15-4. Click the icon “Para” and set the parameters. (Baseline
Factor, Peak Threshold, Calculate…etc.).
16. Click “Run” to start the process.
During Calibration
Please check the Alignment Marker has been located
at correct position.
Software will recognize two peaks from Alignment
Marker signal. DO NOT use Size Marker or DNA sample
doing “Calibrate”.
Brief introduction of the Signal Chart:
Fluorescence Intensity (red line)
Current Unit
Related Fluorescence Unit
Current (gray line)
Unstable
①②③ ④
blue
green
red
During the “HV Check”, the
last LED will show red light
Blue→ SD card
green→ WiFi connectable
red→ HV check