BiOptic Qsep1 User manual

Operation Quick Start
Bio-Fragment Analyzer: Qsep1Document No.: F0016
Ver.: ENG-F
Page: 1/3
1. Ensure the protecting foam is removed (Fig. 1 & 2).
2. Plug the power cord (labeled Qsep1) and Ethernet
cable (optional).
*Scan the QR code for instruction video.
4. Switch on Qsep1, the LED lights up (multiple color
light) (Fig. 3). Wait 25 seconds until the instrument
and Wi-Fi initialized, and the green LED will start
flashing (Fig. 4).
-Check if the AP source is available for Wi-Fi network
on the computer (Fig. 5).
-Find the SSID named with “Qsep1+instrument ID
(16 digits) ”and click “Connect” (Fig. 6).
-Enter the password:
The password will show in the sticker which is
behind the instrument. It is the same as the middle
8 digits of instrument serial number (same as SSID
name) without the prefix “Qsep1”(Fig. 7).
3. Connect the air tube.
*The green flashing LED shows the instrument is
ready to connect. If not, please repeat step 4.
Wi-Fi connection: follow the steps below to
connect with Qsep1
Fig. 5
*DO NOT switch on the instrument immediately
after powering off. Wait at least 5 seconds.
Ethernet cable connection:
refer to step 5 on Page 2
Ethernet cable
Fig. 3
Fig. 6
*If the computer cannot find the Qsep1SSID, keep
the distance between Qsep1and the computer
within 10 meters and start from step 4 again.
*If the computer cannot connect with Qsep1,
-Check the Wi-Fi IP assignment is “Automatic
(DHCP)”
-Initiate the software as administrator
-Allow Q-Analyzer pass through firewall or turn
off the firewall
Fig. 4
Blue
Green
00923718
Fig. 7
Fig. 1 Fig. 2

8-well Sample tray 12-well Sample tray
Operation Quick Start
6. Double click the icon of Q-Analyzer.
7. Q-Analyzer user interface:
Cartridges
Alignment Maker
Separation Buffer
Dilution Buffer
Mineral Oil
Buffer Tray
Dropper
0.2 ml tubes
Bio-Fragment Analyzer: Qsep1Document No.: F0016
Ver.: ENG-F
Page: 2/3
Packing List of Cartridge Kit (Cat. C105200)
5. Check the LED status.
7-2. Click “Connect”, the Qsep1image will change
the color when it’s connected.
8. Prepare buffer and Alignment Marker in 0.2ml PCR tubes and
allocate them at the corresponding position on the sample tray.
*For 12-well sample tray, user needs to use
0.1ml tubes (C104252) for samples and size marker
*S/P/C well can only place 0.2ml PCR tubes
7-1. Click “Search”, and the instrument ID will display.
*Please confirm the instrument ID is the
connected instrument
9. Click “Open Sample Door”, and the sample door will open
automatically.
10. Slowly slide the sample tray into
the instrument and make sure
sample tray is pushed to the end.
12. Open the cartridge door by pressing the white button
and insert cartridge (guiding groove facing front).
11. Click “Park”, and the sample tray
will move into instrument.
*Please follow cartridge unpacking guide to unpack the
cartridge before use
Guiding groove
13. Close the cartridge door and click “Latch”.
*The cartridge information will be displayed after latch.
Green
Red
Blue →On
Green →Wi-Fi connectable
Red →HV on
When the Qsep1connected with the computer, the
green LED will flash longer.
*Make sure there is no air bubble in the tubes
Blue

Operation Quick Start
15-3. Click on “Result Name ”and enter the name of result files.
14. Cartridge Calibration:
New cartridge needs to be calibrated before use.
Please follow the steps below to proceed.
14-1. Click “HV Check”.
14-2. Click “Calibrate”.
* The storage and transportation
condition may influence the Gel-
matrix and cause unstable
current. During HV Check, check
current (gray line) and see if it’s
stable. If current is unstable,
please repeat this step 2-3 times.
* For troubleshooting, please refer to cartridge unpacking
guide for details.
Bio-Fragment Analyzer: Qsep1Document No.: F0016
Ver.: ENG-F
Page: 3/3
*The “Calibrate” button will
only display after HV check
passed.
15-1. Choose the appropriate Alignment Marker and click the
“Sample Position”. Mark the position of sample on the
plate and then click “OK”.
15-2. Click “Method” to select analysis method.
15-4. Click the icon “Para” and set the parameters.
(Baseline Factor, Peak Threshold, Calculate, etc.)
16. Click “Run” to start the analysis.
Brief introduction of the Signal Chart:
Fluorescence Intensity (red line)
Current Unit
Related Fluorescence Unit
Current (gray line)
Unstable
Please check the Alignment Marker has been placed in
correct position.
Software will recognize peaks from Alignment Marker
signal. DO NOT use Size Marker or DNA sample doing
“Calibrate”.
Contact Information:
Company Name: BiOptic Inc.
Office Address: (23141) 5F., No.6, Ln. 130, Minquan Rd., Xindian Dis., New Taipei City, Taiwan
Factory Address: (23141) 4F., No.108-3, Minquan Rd., Xindian Dist., New Taipei City, Taiwan
Tel: [phone], Fax: [phone], E-mail: s[email protected]
Note: if user want to move and pack the instrument,
please click “Lock” before turning off the power.
This command has the motor move to proper
position to prevent shipping damage.
15. Click on the blank column and ①designate the
sample locations, ②test method, sample
duration, runs, ③result name and ④Para by
following steps 15-1, 15-2, 15-3 and 15-4.