BiOptic Qsep1-Plus User manual

Operation Quick Start
Bio-Fragment Analyzer: Qsep1-Plus Document No.: F0081
Ver.: ENG-A
Page: 1/3
1. Ensure the SD card is properly inserted in the
socket (Fig. 1 and 2).
3. Plug the power cord (labeled Qsep1-Plus)
*SD card needs to be inserted before powering on.
The SD card CANNOT be initialized with hot plugging.
4. Switch on Qsep1-Plus, the LED lights up (multiple
color light) (Fig. 3). Wait for 25 seconds until the
instrument and Wi-Fi initialized, and the green LED
will start flashing (Fig. 4).
-Check if the AP source is available from your Wi-Fi
network in the computer (Fig. 5).
-Find the SSID named with “Qsep1+instrument ID
(16 digits) ” and click “Connect” (Fig. 6)
-Enter the password:
The password will show in the sticker which is
behind the instrument. It is the same as the middle
8 digits of instrument serial number (same as SSID
name) by blinding the prefixal “Qsep1” (Fig. 7).
2. Connect the air tube.
*The green flashing LED shows the instrument is
connected. If not, please repeat the step 4.
Wi-Fi connection: follow the steps below to
connect with Qsep1-Plus
Fig. 5
*DO NOT switch on the instrument immediately
after powering off. Wait at least 5 seconds
Ethernet cable connection:
refer to step 5 on Page 2
Ethernet cable
Fig. 6
*If the computer cannot find the SSID of Qsep1-Plus,
keep the distance between Qsep1-Plus and the
computer within 10 meters and start from step 4
again.
*If the computer cannot connect with Qsep1-Plus
-Please check the wi-fi IP assignment is “Automatic
(DHCP)”
-Allow Q-Analyzer pass through firewall or turn off
the firewall
Fig. 3
Fig. 4
Blue
Green
00923718
Fig. 7
Fig. 2
Fig. 1

8-well sample tray 12-well sample tray
Operation Quick Start
6. Double click the icon of Q-Analyzer.
7. Q-Analyzer user interface:
Cartridges
Alignment Maker
Separation Buffer
Dilution Buffer
Mineral Oil
Buffer Tray
Dropper
0.2 ml tubes
Bio-Fragment Analyzer: Qsep1-Plus Document No.: F0081
Ver.: ENG-A
Page: 2/3
Packing List of Cartridge Kit (Cat. C105200)
5. Check the LED status.
7-2. Click “Connect”, the Qsep1-Plus image will
change the color when it’s connected.
8. For 8-well and 12-well sample tray, prepare buffer and
Alignment Marker in 0.2ml PCR tubes and allocate at the
corresponding position on the sample tray.
*For 12-well sample tray, user needs to use
0.1ml tube (C104252)for sample and size marker
*S/P/C well can only place 0.2ml PCR tubes
*Make sure no air bubble appears in each tube
7-1. Click “Search”, and the instrument ID will display.
*Please confirm the instrument ID is the
connected with instrument
For 16+3-well sample tray, please use 16+3 Sample Tube
(C104254) and follow the instruction below to load the buffers,
markers and samples.
9. Click “Open Sample Door”, and
the sample door will open
automatically.
10. Slide the buffer tray into the
instrument. Make sure sample
tray is pushed to the end.
12. Open the cartridge door by pressing the white button
and insert cartridge (guiding groove facing front).
11. Click “Park”, and the sample tray will move into
instrument.
*Please follow cartridge unpacking guide to unpack the
cartridge before using
Guiding groove
13. Close the cartridge door and click “Latch”.
*The cartridge information will be displayed after latch.
Blue
Green

Operation Quick Start
15-3. Click on “Result Name” and enter the name of
result files.
14. Cartridge Calibration:
New cartridge need to be calibrated before use.
Please follow the steps below to proceed.
14-1. Click “HV Check”.
14-2. Click “Calibrate”.
* The storage and transportation
condition may influence the Gel-
matrix and cause unstable current.
During HV Check, check current
(gray line) and see if it’s stable. if
current is unstable, please repeat
this step 2-3 times.
* For troubleshooting, please refer to cartridge unpacking
guide for details.
Bio-Fragment Analyzer: Qsep1-Plus Document No.: F0081
Ver.: ENG-A
Page: 3/3
*The “Calibrate” button will
only display after HV check
passed.
15. Click on the blank column and designate ①the sample
locations, ②test method, sample duration, runs, ③result
name and ④Para by following steps 15-1, 15-2, 15-3 and 15-4.
15-1. Click the “Sample Position”, mark the position of sample on
the plate and then click “OK”.
15-2. Click “Method” to select analysis method.
15-4. Click the icon “Para” and set the parameters.
(Baseline Factor, Peak Threshold, Calculate, etc.).
16. Click “Run” to start the process.
Brief introduction of the Signal Chart:
Fluorescence Intensity (red line)
Current Unit
Related Fluorescence Unit
Current (gray line)
Unstable
During the “HV Check”, the
last LED will show red light
Blue → SD card
Green → WiFi connectable
Red → HV check
Blue
Green
Red
Please check the Alignment Marker has been placed in
correct position.
Software will recognize peaks from Alignment Marker
signal. DO NOT use Size Marker or DNA sample doing
“Calibrate”.
* If you want to analyze the samples with Alignment Marker, please
choose the appropriate Alignment Marker and then place it in the
corresponding position. (Check ✓the box)
Contact Information:
Company Name: BiOptic Inc.
Office Address: (23141) 5F., No.6, Ln. 130, Minquan Rd., Xindian Dis., New Taipei City, Taiwan
Factory Address: (23141) 4F., No.108-3,Minquan Rd., Xindian Dist., New Taipei City, Taiwan
Tel: [phone], Fax: [phone], E-mail: [email protected]