BRIGHT 5040 User manual

Revised May 2012 1
INSTRUCTION MANUAL
5040 Wax Microtome/Quick Release Feather Blade Holder
Serial No: 5560

Revised May 2012 2
INSTRUCTION MANUAL 1
SAFETY INFORMATION 3
Consumer Protection 3
Please Take Note Of The Following Warnings: 3
ELECTRICAL 3
OPERATION 4
ACCESSORIES 4
PRODUCT SAFETY SUGGESTIONS 4
DECONTAMINATION CERTIFICATES 4
IMPORTANT 4
WARRANTY 5
Optional Feedscrews 5040 7
1.2.3 QUICK RELEASE FEATHER BLADE HOLDER BLOCK 8
1.2.4 KNIFE CARRIAGE FEED CONTROL 8
1.2.4a FINE TRIM ADJUSTER / FTA (if fitted) 8
1.2.5 BLADE ANGLE 8
1.2.6 BLADE LOADING 8
1.2.7 SPECIMEN HOLDERS 8
1.2.8 SPECIMEN ORIENTATION (where fitted) 9
1.2.9 ADVANCE / REWIND CONTROL 9
1.2.10a LEFT HAND SIDE ADVANCE/REWIND CONTROL - /LHS (where fitted) 9
MOTOR DRIVE (where fitted) 9
FOOT SWITCH (where fitted) 10
1.2.11 SECTION COUNTER (where fitted) 10
1.2.12 MOTORISED ADVANCE / REWIND (where fitted) 10
1.2.13 OTHER ACCESSORIES 10
2. OPERATION 11
2.1 FITTING THE SPECIMEN 11
2.2 TRIMMING THE SPECIMEN 11
2.3 SECTION CUTTING AND COLLECTION 11
2.4 HINTS ON SECTIONING 11
3.1 DAILY CARE 12
3.2 MAINTENANCE & SERVICING 12
3.3 DECONTAMINATION 12
4. SPECIFICATION 12
ORDERING INFORMATION 14
OPTIONAL FEATURES 14
5. SPARE PARTS AND ACCESSORIES 15
5.2 5040 Electronics Spares List 17
5.3 Accessories and Consumables 18
A KNIFE SHARPENING SERVICE IS AVAILABLE - DETAILS ON REQUEST 18
MATERIAL SAFETY DATA SHEET 19
Name: CRYO-M-BED 19
MATERIAL SAFETY DATA SHEET 20
Name: CRYOSPRAY 20
MATERIAL SAFETY DATA SHEET 21
Name: LOW TEMPERATURE OIL 21
WARNINGS: Low Temperature 26
Knives/Blades 27
DECONTAMINATION CERTIFICATE 28
DECONTAMINATION PROCEDURES 29
QUALITY SURVEY REPORT 30

Revised May 2012 3
SAFETY INFORMATION
Consumer Protection
The Consumer Protection Act 1987 Part 1. refers to Product Liability. This legislation was issued as a direct result of an
EC Directive to all member states and has been in force with effect from 1 March 1988.
The Bright Instrument Company Limited, ever mindful of the need to ensure that their products are not subject to misuse
and/or incorrect handling, have made it their aim to communicate any possible dangers to their customers.
Whilst the Bright Instrument Company Limited markets products manufactured to the highest safety standards, it is in the
interest of the purchaser that he is aware of the resultant dangers of misuse and/or incorrect handling of these products.
Please Take Note Of The Following Warnings:
ELECTRICAL
a) Warning - A warning notice is fixed to the instrument (where applicable) stating that it should be disconnected
from the mains supply before removing the panels. This warning should be strictly observed.
b) Fuses – Fuse ratings are clearly indicated on all fuse panels adjacent to the fuse holder. If and when
replacement is necessary, the correct fuse rating and type must be adhered to.
c) Earthing (Grounding) – A protective earth terminal is fitted and must be used in all two-wire installations.
MICROTOME KNIVES/BLADES
The microtome knife/blade can be a hazard in the laboratory. Personnel should be made aware of the dangers and
observe the following warnings:
a) DO NOT leave the microtome unattended with an exposed knife/blade in position. Remove the knife/blade, or
cover with the guards or visor provided.
b) DO NOT leave unboxed knives/blades lying around. Place knives/blades that are not in use in their boxes or
packets.
c) DO NOT carry knives/blades unless secure in the box or packet provided.
d) DO NOT clean the knife/blade along its length. Wipe the knife from the back edge to the cutting edge.
e) REMEMBER that even used knives and blades are dangerous. The are still sharp and may have been used to
cut potentially infectious specimens.
f) DISPOSE of used knives and blades with the same care as other sharp objects. On no account should used
knives or blades be placed in waste bins.

Revised May 2012 4
OPERATION
When placing specimens in the microtome vice clamp jaws or holder, or carrying out manipulation, ensure the cutting
hand wheel is locked with the head in the uppermost position, to minimise risks.
LOW TEMPERATURES
Low temperatures are present in cryostats and in freezing head stages attached to microtomes. On no account should
you touch these cold surfaces without the use of gloves or sufficient clothing covering your exposed wrists/arms etc.
touching cold surfaces can result in cold “burns” and permanent tissue and nerve damage.
ACCESSORIES
Fluids supplied as accessories with Bright instruments, such as Cryospray 134 and microtome oil are strictly for
laboratory use only. They should not be taken by mouth and precautions afforded to other laboratory chemicals should
be adhered to.
PRODUCT SAFETY SUGGESTIONS
All Bright Instrument Company Limited personnel are encouraged to make suggests regarding product safety. We also
welcome such suggestions from our customers. They may be submitted by completing the appropriate (Safety) section
of the Quality Survey Record Form supplied with all Bright instruments, or alternatively, by letter, telephone, fax or email.
All communications should be direct to our Quality Assurance Department and will be acknowledged.
DECONTAMINATION CERTIFICATES
IMPORTANT
If the instrument or any part of it is to be returned to Bright Instrument Company Limited, a decontamination certificate
must accompany it, and please note the following:
a) If the instrument or any part of it has been exposed to or been in contact with potential pathogenic or radioactive
material, it is essential that it be decontaminated.
Set procedures are laid down by the Health and Safety Executive for decontamination. For the avoidance of
doubt, we ask that instruments or parts returned to us should be accompanied by a completed decontamination
certificate. A copy of this can be found at the back of this instruction manual and we suggest you use a
photocopy of this when returning parts.
Alternatively we would be pleased to:
b) either post or fax you another copy should you require.
c) Should the instrument or any part of it be received in a condition that Bright Instrument Company Limited,
consider to be a potential biological hazard, the instrument or part will be returned un-repaired at the expense of
the customer.
d) Overseas customers declarations must indicate that the package contains ‘British Returned Goods’. Failure to
do so will involve customs duty payable by us, which will be invoiced to the sender.

Revised May 2012 5
WARRANTY
The Warranty relating to the instrument is outlined in our Terms and Conditions of Sale paragraph 6.
The Seller ‘s products are carefully inspected and submitted to its standard tests.
The Seller warrants all its products to be free from defects in workmanship and materials under normal conditions of use
and service provided always:
a) that if any of the goods so manufactured is alleged to be defective in workmanship and material and is returned
carriage paid, and protected against damage in transit to the Seller’s works at Huntingdon within 12 months
from the date of despatch and if after examination by the Seller that goods or part of them are found to be so
defective then the Seller will repair or replace them free of charge and will return them to the Buyer , carriage
paid.
b) where any part of the goods manufactured by the Seller is repaired or replaced under the terms of the foregoing
warranty, such warranty shall thereafter be limited to a period of six months from the date when the goods shall
have been re-delivered to the Buyer.
c) this warranty does not apply to any defects caused by wear and tear, incorrect installation abnormal conditions
of working, accident, misuse or neglect.
d) That save as in this clause herein before expressed, the Seller shall not be under any liability for negligence or
otherwise in respect of defects in goods delivered or for any injury, damage or loss resulting from such defects
and the Seller’s liability under this clause shall be in lieu of any warranty or condition implied by law as to the
quality or fitness for any particular purpose of such goods.
e) This warranty is expressly in lieu of all other warranties, guarantees or liabilities expressed or implied by any of
the Seller’s Representatives or Agents.
Please see our separate Product Warranty sheet for deliveries to the mainland UK.
WARNING
Before proceeding to Operating Instructions, ensure you are familiar with the contents of the pages marked
‘Safety Information’. This instrument must only be used by competent persons.

Revised May 2012 6
OPERATING INSTRUCTIONS
1) INTRODUCTION
The Bright 5040 is a rotary rocking microtome with specimen retraction on the return stroke. In standard form it
can cut sections up to 60µm thick and will accept specimens up to 50 x 70mm.
Suitable for paraffin wax and plastic embedded specimens, the microtome is fitted with a standard specimen
vice clamp or a quick release holder for cassettes, A wide range of options and accessories are available,
including motor drive and feed screws for cutting sections up to 300µm
Knife/Blade guards are fitted to protect the operator from the knife/blade during normal operation. All controls
are also placed clear of the knife/blade to permit all settings to be made in comfortable safety.
1.1 RECEIPT AND UNPACKING
All packing must be carefully removed and parts checked against enclosed packing list. If any damage or
discrepancy is noted, please inform our agent/distributor or Bright Instrument Company Limited immediately.
1.1.2 UNPACKING PROCEDURE
The following should be followed when the instrument is received:
a) At least two people are required during the unpacking. The microtome weighs 15Kg.
b) Check that the outer packing is in good order and does not show signs of serious damage.
c) Remove the microtome from the case and unpack it.
d) Remove and unpack the accessories.
FITTING THE CUTTING HAND WHEEL
Push the hand wheel FIG 1(1) over the shaft on the side of the microtome. Ensure pin on shaft engages slot in
centre of hand wheel, press home firmly then fit centre screw.
1.1.3 FITTING THE COARSE ADVANCE/REWIND CONTROL
The rewind knob is fitted FIG 1(3) into the top of the microtome, ensuring it engages properly.
1.2 FEATURES
Refer to FIGs 1 and 2.
1.2.1 CUTTING HAND WHEEL
The hand wheel can be locked with the cutting arm in the upper position. Move the handle on the wheel to the
top, pull the small lock knob FIG 1(2) and rotate it quarter of a turn, release and ensure it locates correctly.
To operate the microtome, release the lock and turn the hand wheel in a clockwise direction.
1
3
FIG 1
24
5
6

Revised May 2012 7
1.2.2 THICKNESS CONTROL
The knob FIG 1(5), on the front of the microtome, is used to set section thickness in µm. Simply turn the knob
to the desired setting
FEEDSCREWS
A range of alternative feedscrews is available to cover section thickness’ from 0.2µm to 300µm. If one of these
has been fitted in the factory, an appropriate scale of microns will also be fitted where available. However, if the
user substitutes an alternative himself, the following table should be used to obtain the current section
thickness.
Optional Feedscrews 5040
Optional
Feedscrews 5040
50256 manual
50256-1 MAR
50255 manual
50255-1 MAR
50257 manual
50257-1 MAR
50258 manual
50258-1 MAR
50259 manual
50259-1 MAR
50260 manual
50260-1 MAR
“Thin Section” Standard in cryostat Standard in wax
microtome
0.2 to 12µm 0.5 to 30µm 1 to 60 µm 2 to 120µm 3 to 180µm 5 to 300µm
0.2µm incrs. 0.5µm incrs. 1 µm incrs. 2µm incrs. 3µm incrs 5µm incrs.
With scale With scale With scale Without scale Without scale Without scale
Scale Markings:
2 5 10 20 30 50
4 10 20 40 60 100
6 15 30 60 90 150
8 20 40 80 120 200
10 25 50 100 150 250
12 30 60 120 180 300
Note: MAR = Motorised Advance/Rewind
FIG 2 5040 QUICK RELEASE FEATHER BLADE HOLDER
1. Blade clamp lever
2. Blade angle locking lever
3. Blade angle indicator
4. Blade guard
5. Blade loading plate
6. Fixed blade guard
7. Blade guard knob
8. Blade clamping plate
9. Knife block clamp locking lever
1
2
3
45
6
6
7
9
8

Revised May 2012 8
1.2.3 QUICK RELEASE FEATHER BLADE HOLDER BLOCK
The complete knife holder block FIG 1(6) can be moved towards or away from the specimen. Raise the knife block clamp
locking lever FIG 2(9) on the right side of the knife holder and slide the holder to the desired position, or use knife
carriage feed control. Note that the knife carriage feed control can be replaced by the FTA (Fine Trim Adjuster 1.2.4a)
Press the lever down to lock.
In normal paraffin was section cutting, the knife holder would seldom be moved once it has been put in the most
convenient position for the size of the block.
NB: The knife block clamp lever must be firmly locked during sectioning to prevent instability.
1.2.4 KNIFE CARRIAGE FEED CONTROL
The knife carriage feed control FIG 1(4) on the centre front of the microtome moves the knife holder backwards or
forwards once the knife lock clamp lever FIG 2(9) has been raised. This device permits incremented movements of the
knife holder, for example, for trimming a specimen.
1.2.4a FINE TRIM ADJUSTER / FTA (if fitted)
Works exactly the same as the knife carriage feed control
1.2.5 BLADE ANGLE
Release the blade angle locking lever FIG 2(2) and taking the body of the blade holder in both hands, move it against the
lower part of the assembly until the correct angle is shown on the blade angle indicator (3). Then re-lock the blade holder
body with the blade angle locking lever. Nominally this angle should be set to 3º.
1.2.6 BLADE LOADING
a. Move the blade guard to the lower position using the blade guard knob FIG 2(7).
b. Unlock the blade clamping plate FIG 2(8) using the blade clamp lever FIG 2(1).
c. Push the blade loading plate FIG 2 (5) to one side and very carefully place a new blade into position making sure that
the blade is the correct way up.
d. Slide the blade loading plate back to the centre of the assembly and use the blade clamp lever to lock the blade into
position. Please note that as the blade wears it is possible to slide it to a new position by repeating this process and by
doing this it is possible to utilise the whole length of the blade.
1.2.7 SPECIMEN HOLDERS
318 Quick Release Holder for wax cassettes
The microtome is normally supplied with an orientating specimen holder with a specimen vice
Alternatively, a cassette quick release holder may be fitted. To exchange specimen holders, simply
unscrew the holder supplied from the orientating head.
Some versions of the microtome are supplied with a fixed (non-orientating) specimen vice. In this case
a cassette quick release holder can be clamped into this vice.
Other types of specimen holders are available.
FTA (Cryostat
version)

Revised May 2012 9
1.2.8 SPECIMEN ORIENTATION (where fitted)
To change the orientation of the specimen, first unlock the clamping lever, then either turn
the adjusting knobs, or move the specimen holder by hand.
NOTE: Before attempting to change the orientation ensure cutting hand wheel is locked.
1.2.9 ADVANCE / REWIND CONTROL
The Advance / Rewind control (on top of the microtome) is
used to advance the specimen to the knife and for trimming. It is also used to reset the
mechanism usually after cutting each specimen.
NOTE: the control cannot be rewound if the specimen is at the top of the cutting stroke;
either lower the specimen or set the thickness control to 0µm to disengage the ratchet
mechanism.
A used feed indicator on the front of the microtome shows how much of the specimen-advance
remains. Rewind the mechanism when the white pointer reaches the red zone, by turning the knob on
top of the microtome clockwise, or if Motorised Advance / Rewind is fitted then see paragraph 1.2.13
1.2.10a LEFT HAND SIDE ADVANCE/REWIND CONTROL - /LHS (where fitted)
The operation is exactly the same as for the Advance/Rewind control on the top of the microtome. Please note that this
option is not available with Motorised Advance/Rewind
1.2.10 MOTOR DRIVE (where fitted)
The motorised cutting system on the 5040 microtome is used for cutting large or hard specimens, and is also useful for
serial sectioning. The system can be disengaged for manual cutting.
1. Ensure the specimen will not collide with the knife.
2. To engage motor drive, pull and turn hand wheel plunger knob.
Then slowly rotate hand wheel until plunger engages. The hand
wheel is locked in motor drive. See section 1.2.1 for manual use.
3. Switch on drive. The green power light will illuminate.
4. Select trim mode (for continuous running of motor drive) press
start button (green)
5. Use the speed control to adjust cutting speed, when the head is in
the cutting zone.
6. Adjust the zone control. The space between the upper and lower
positions indicates that part of the specimen travel which is
determined by the speed control.
7. By switching to single, the arm will be made to stop at the bottom
of each stroke, allowing section collection.
8. To stop the drive, press stop. Start and stop functions can be used at any time.
9. The emergency-stop button stays in when pressed. Twist button in direction of arrows to release. Then press
start to continue drive.
LHS (Cryostat
version)

Revised May 2012 10
FOOT SWITCH (where fitted)
Plug the foot switch into the socket on the lower front surface of the motor drive control box. When the motor drive is in
use, the left and right foot switches will duplicate the action of the stop and start buttons respectively.
1.2.11 SECTION COUNTER (where fitted)
The section counter is used to pre-set the number of cutting stokes from 1 to 9,999 strokes. Press the buttons on the
counter until the required number of sections is displayed, then press the reset button.
For each cutting stroke the counter display will increase by one, giving the number of sections cut until it reaches the pre-
set value when the red up light will illuminate and the drive will stop. To repeat the cycle, press the reset and start
buttons.
1.2.12 MOTORISED ADVANCE / REWIND (where fitted)
Do not operate the rewind motor when the cutting arm is in motion. Only operate the rewind motor
with the cutting arm at the rest position. The advance motor may be operated when the cutting arm
is in motion. Operating outside these parameters can cause damage to the mechanical
components of the machine.
MOTORISED SPECIMEN ADVANCE
1. Set the Specimen thickness control to 0µm
2. Fully rewind specimen, by pressing the rocker switch in the appropriate direction.
3. Adjust position of knife carriage with knife to specimen clearance set to a minimum.
4. Lock knife carriage.
5. Begin advance, with cutting arm fully up, by pressing and releasing ‘advance’ switch whilst rotating the hand
wheel. The longer the switch is pressed the thicker the section. If ‘advance’ switch is pressed for more than 2
seconds, a faster advance of the specimen is achieved.
MOTORISED SPECIMEN REWIND
1. Fully rewind specimen press ‘rewind’ switch for a minimum of 2 seconds and release. The feedscew will
automatically stop when fully rewound.
2. To stop the specimen rewinding during the period, press ‘advance’ switch and release immediately.
3. For small rewind increments, press and quickly release ‘rewind’ switch.
1.2.13 OTHER ACCESSORIES
Debris Tray: Use this tray to collect section trimmings etc:
Alternative Feed Screws: See section 1.2.2 Instructions for fitting the alternative feed screws are supplied with the
feed screws.

Revised May 2012 11
2. OPERATION
2.1 FITTING THE SPECIMEN
Ensuring the cutting hand wheel is locked and the blade guard is covering the blade. Fit the specimen into the specimen
holder. If the standard vice clamp is used, ensure the specimen is tightly clamped without damaging it. Alternatively, if
the cassette quick release is used, care should be taken to ensure a close fit to the cassette. Remove all excess wax.
Cassettes may be fitted vertically or horizontally.
2.2 TRIMMING THE SPECIMEN
Release the knife block clamp locking lever FIG 2(9) and move the knife holder close to the specimen (see section
1.2.3) Re-lock the lever.
Either set the section thickness control to thick sections, eg: 15-20µm (see section 1.2.2) and turn the hand wheel to trim
the specimen.
OR rotate/rock the hand wheel while using the coarse advance (see section 1.2.4) to trim the specimen.
NOTE: If rocking the hand wheel, ensure trimming is done on the down stroke (hand wheel knob is 1 o’clock round to 5
o’clock position). Trimming on the upward (retraction) stroke will result in a subsequent very thick section and may
damage the specimen.
2.3 SECTION CUTTING AND COLLECTION
Select the desired section thickness. Turn the cutting hand wheel a few times to remove any surface roughness on the
specimen block caused by trimming.
Cut sections using slow, steady rotation of the hand wheel. Wax-embedded specimens should form a ribbon of sections
on the blade.
If the sections keep rolling then make sure that the wax blocks are still cool (+5ºC is the best temperature for most wax).
If they continue to roll then try gently blowing down onto the knife to turn down the leading edge of the section.
Sections can be removed either singularly or as a ribbon using forceps. Wax sections are usually floated onto warm
water to soften and flatten them.
They can be collected on microscope slides as follows:
a. Dip a microscope slide vertically into the warm water some distance from the sections.
b. Carefully bring the slide close to the section so that the sections edge contacts the slide in the appropriate
position.
c. Now raise the slide vertically from the water.
2.4 HINTS ON SECTIONING
1. Ensure the knife is sharp and clean. During use, the knife should be cleaned occasionally to remove a build-up
of wax. Buff with a paper towel away from the edge of the knife.
2. Wax embedding specimens usually cut more easily if they have been chilled. It is common practice to trim a
batch of wax blocks, then place them face down on a cold plate or crushed ice to cool them. This way they can
be returned to the microtome and sections cut while they are still cold.
3. A slow, steady cutting action is needed to obtain the best results. However, different specimens may benefit
from different cutting speeds, so experimentation will sometimes bring improved results.
4. If the section ribbon is excessively folded, creased or compressed on the knife, first float it onto cold water and
flatten it with the aid of a small artist’s brush. Now collect sections with a microscope slide and carefully re-float
onto warm water.
5. After collection, dry the wax sections onto the slides using either an oven or a hot plate. Using temperatures
10º-20º above the melting point of the wax. Sections will flatten and stick to the slide without being damaged.
For more detailed instructions on the preparation, cutting and subsequent staining of paraffin wax sections, see any
conventional text book of histological technique.

Revised May 2012 12
3. MAINTENANCE
No routine maintenance is required.
3.1 DAILY CARE
Empty section debris from the plastic debris tray. Brush away debris from around microtome. Ensure knife is kept sharp
and free of nicks.
No other daily attention is required.
3.2 MAINTENANCE & SERVICING
The microtome requires no routine maintenance. In the event of problems, first refer to section 2.5. If no sections are
being cut, ensure microtome feed has been rewound (see section 1.2.10).
For advice on repairs, contact either Bright Instrument Co Ltd or your local representative, giving details of the fault and
quoting the serial number of your instrument.
3.3 DECONTAMINATION
It is the responsibility of the user to ensure that a decontamination procedure is employed which is appropriate to the
nature of the work carried out.
The 5040 Sledge Microtome is constructed of corrosion resistant materials, but hypochlorite (bleach) solutions are
corrosive to many metals and should be avoided.
4. SPECIFICATION
Construction
Section thickness
Range
Specimen advance
Knife block
movement
•Rotary rocking design of cast alloys, with detergent and solvent resistant epoxy-polyester
finish
•Standard range 1-60µm in 1µm steps for wax microtomes and 0.5-30µm in 0.5µm steps for
the cryostat version. – See table below
•Total specimen advance is 5600µm.
•Total knife block movement is 44mm.
Retraction
•Approximately 50µm
Cutting stroke •56mm
Specimen orientation
•± 8º horizontal and vertical (standard on 5040-01)
Remaining feed
Indicator
•Visual display
Knife holders •Standard knife holder accepts conventional steel knives; tungsten carbide tipped knives,
glass knives and disposable blade holders. Alternatively, the quick release Feather blade
holder is available as an optional feature either in addition to or in place of the standard
knife holder block.
Motorised section
cutting
•(Optional) With variable speed, variable cutting zone, single or continuous modes, hands off
footswitch and emergency stop.
Safety features •Counter-balance handwheel with safety lock. Integral knife guards, sliding both ways to
cover knife edges
•
Dimensions •H300 x D500 x W300
•Net weight (manual): 20kg
•Net weight (motorised): 30kg
•Gross weight (manual): 25kg
•Gross weight (motorised): 35kg
•Shipping volume: 0.158m3
•Packing dimensions: H580 x D680 x W400mm
•
Note: Weights vary according to specification of instrument

Revised May 2012 13
OPTIONAL FEEDSCREWS 5040
Optional
Feedscrews 5040
50256 manual
50256-1 MAR
50255 manual
50255-1 MAR
50257 manual
50257-1 MAR
50258 manual
50258-1 MAR
50259 manual
50259-1 MAR
50260 manual
50260-1 MAR
“Thin Section” Standard in cryostat Standard in wax
microtome
0.2 to 12µm 0.5 to 30µm 1 to 60 µm 2 to 120µm 3 to 180µm 5 to 300µm
0.2µm incrs. 0.5µm incrs. 1 µm incrs. 2µm incrs. 3µm incrs 5µm incrs.
With scale With scale With scale Without scale Without scale Without scale
Scale Markings:
2 5 10 20 30 50
4 10 20 40 60 100
6 15 30 60 90 150
8 20 40 80 120 200
10 25 50 100 150 250
12 30 60 120 180 300
Note: MAR = Motorised Advance/Rewind

Revised May 2012 14
ORDERING INFORMATION
Order Code Description
5040-01 5040 Rotary Microtome with fine
adjustment specimen orientation
5040-02 5040 Rotary Microtome without
specimen orientation
5040-CV 5040 Rotary Microtome for cryostats
OPTIONAL FEATURES
Order Code Description
215 Knife carriage feed screw mechanism
217 Micro-adjustable knife angle mechanism
318 Quick release holder
53613 Supercassette quick release holder
225MR Robomatic motor drive for 220/240V AC, 50/60Hz
226MR Robomatic motor drive for 110/115V AC, 50/60Hz
225MR(S) Robomatic motor drive as
225MR but for very hard specimens
226MR(S) Robomatic motor drive as
226MR but for very hard specimens
/C Electronic section counter
/C(M) Mechanical section counter
/V Foot switch for motor drive
/Ma/r Motorised advance rewind
/LHS Advance/rewind handle on left-hand side front
/FTA Fine trim adjuster control
/LC Cryostat type handwheel lock (not available with motor drive)
53430 Quick Release Feather Blade Holder
57903 Lever Release Feather Blade Holder

Revised May 2012 15
5. SPARE PARTS AND ACCESSORIES
Parts can be obtained through your local Bright representative, or from Bright Instrument Co Ltd. When ordering parts, please provide the following details:
a Model type and serial number of your instrument
b Full description, part number and quantity of part(s) required
c Address to which parts are to be delivered
d Address to which invoice is to be sent
5.1 5040 Parts List
Item Number Quantity Part Number Description
1 1 50384 Anti-roll assembly
2 1 50442 Knife clamp tie-bar
3 1 50431 Extension
4 4 50984 Knob
5 1
50983 Knob
6 1 50432 Adjusting screw
7 1 50399 Locating pin
8 2 50351 Knife clamp screw
9 3 50986 Knob
10 1 50406 Knife clamp, left hand
11 2 51344 Grub screw
12 1 52442 Knife guard assembly, left hand
13 2 52395 Lock screw
14 1 52355 Knife block
15 1 52443 Knife guard assembly, right hand
16 2 52203 Bush
17 1 52378 Push rod
18 1 52379 Push rod end
19 1 55000 Grub screw
20 1 50526 Knife angle pointer
21 4 55000 Grub screw
22 1 50410 Connecting crank
23 2 55600 PTFE washer
24 1 50357-2 Con rod assembly
25 1 50411 Crank pin
26 1 50407 Crank plate
27 1 50405 Knife clamp, right hand
28 1 50430 Clamp lever
29 1 50471 Stop pin
30 1 55001 Grub screw
31 1 50412 Pawl drive rod
32 1 50413 Pawl pivot
33 1 50414 Pawl spring
34 1 52439 Pawl
35 1 52380 Push rod holder
36 2 55241 Cap head screw
37 2 55616 Washer
38 1 55104 Cap head screw
39 1 55003 Grub screw
40 1 52390 Top arm pivot pin
41 1 52372 Pivot arm rod
42 1 50255 Feed screw assembly
43 1 50404 Bearing post
44 1 50416 Thickness plate
45 1 50459 Knock off arm
46 2 55601 Ball
47 5 55101 Cap head screw
48 1 50458 Retaining plate
49 1 50457 Spring plate
50 1 52371 Pivot arm adaptor
51 1 55610 Washer
52 1 50476 Clamp plate
53 1 50450 Locating pin
54 1 50449 Vee block
55 1 50353 Vice clamp screw
56 2 52427 Blind clamp spring
57 2 52427 Top blind clamp
58 2 53428 Top blind clamp screw
59 1 50987 Rewind knob
60 1 55222 CSK SLTD screw
61 1 50363 Rewind handle
62 1 52348 Top arm (std)
63 1 55108 Cap head screw
64 2 50370 Spring anchor pin
65 1 55621 Washer
66 1 55627 Double coil washer
67 6 50510 Anti rattle body
68 1 50453-1 Pivot arm
69 1 50372 Stop spacer
70 2 55105 Cap head screw
71 8 50423 Anti rattle joint
72 1 50375 Retraction link rod
73 28 55100 Cap head screw
74 3 50438 Retaining plate
75 1 50447 Main hinge

Revised May 2012 16
76 1 50373 Stop pin
77 1 50454-1 Hinge segment
78 4 50445 Small hinge
79 8 50446 Retaining plate
80 3 56725 Full nut
81 1 55626 Crinkle washer
82 1 50475 Washer
83 1 50507 Anti-rattle body
84 1 55234 Pan head screw
85 1 50451 Pawl arm
86 2 50980 Click block spring
87 2 51443 Click ball
88 1 50417 Thickness block
89 2 55111 Btn head screw
90 2 50991 Pawl arm bearing
91 2 50475 Pawl washer
92 2 51058 Main shaft brush
93 1 56402 Circlip
94 2 55001 Grub screw
95 1 52430 Main drive shaft
96 1 52345 Base
97 2 55241 Cap head screw
98 1 52357 Bearing holder
99 4 52202 Bush
100 1 52361 Thickness collar
101 2 52360 Thickness gear
102 1 52359 Thickness shaft
103 2 52387 Knife block runners
104 1 56700 Thickness pointer
105 1 52358 Thickness knob shaft
106 1 52356 Bearing holder
107 2 55120 Cap head screw
108 3 51443 Ball plunger
109 2 55003 Grub screw
110 1 52362 Thickness ring holder
111 1 52370 Thickness knob
112 1 52363 Thickness ring (std)
113 1 50387 Knob
114 1 52441 Locating pin
115 1 50388 Bearing holder
116 1 50389 Bush
117 1 56403 Circlip
118 1 50392 Leadscrew
119 1 52389 Clamp bolt
120 2 51344 Grub screw
121 1 50378 Lock screw
122 2 50386 Bearing post
123 1 50419 Centre spindle
124 2 55002 Grub screw
125 1 50443 Thickness crank arm
126 1 52388 Knife carriage block
127 2 55508 Nut
128 1 52386 Thickness drive rod
129 1 55105 Cap head screw
130 1 50402 Clamp plate
131 1 52411 Retraction roller
132 1 50401 Retraction roller pin
133 2 50371 Spring
134 2 56725 Nut
135 1 55628 Double coil washer
136 2 50403 Retraction plate
137 2 50376 Spring anchor pin
138 1 50374 Pivot stud
139 4 50391 Plastic feet
140 1 52373 Pivot rod adaptor
141 1 52374 Pivot rod
142 1 52375 Pivot
143 1 55001 Grub screw
144 2 52437 Bush
145 1 52376 Pivot block
146 1 55001 Grub screw
147 1 52377 Pivot rod end
148 1 55106 Cap head screw
149 1 55620 Washer
150 2 55104 Cap head screw
151 1 55630 Washer
152 1 50493 Bottom washer
153 1 50493-1 Spring

Revised May 2012 17
5.2 5040 Electronics Spares List
Part Number Description
52471 5040 control box for wax version
52472 5040 control box top
52474 Facia panel (blank with rewind)
52473-1 acia panel (rewind 230V 0.5AT printed)
52473-2 acia panel (rewind 115V 1.0AT printed)
52474 Facia panel (blank no rewind)
52474-1 acia panel (no rewind 230V 0.5AT printed)
52474-2 acia panel (no rewind 115V 1.0AT printed)
Earth stud 4mm
Earthboltc/s
137043-02 Ring crimp 4mm
134024 Bridge rectifier P1620F
133078 Flex bushing
132106-17 Capacitor RAD 4700MF/40V
133016 Capacitor clip
133064 Feet, rubber grey
138404-19 Fuse 22mm 2.0AT
138404-16 Fuse 22mm 1.0AT
133034 Fuseholder 22mm panel
134028 Lamp, neon green
136002 Motor drive board
136017 Slider board
52339 Slider knob
130200-61 Resistor 0.25W, 100K
137031 Socket IEE for 1 amp
137031 Socket IEE for 1 amp
137023 Socket DIN 5 way 60 deg
137025-02 Socket Dee 15 way
137013-09 Housing Molex 10 way 0.1
137013-14 Housing Molex 15 way 0.1
137013-18 Crimp kk0.1
137013-19 Polarising key
138016 Switch SP rocker
138114-01 Switch emergency stop
138114-03 Sw Em stop contact block
138118-01 Push switch green mom
138118-02 Push switch red mom
137042-15 Receptacle red 1/4 inch
133022-03 Terminal block
138014 Transformer 75 Va
131105 Coiled coil 6 amp
139025 Motor-DC, 36 V/3000 RPM
137026-02 Plug dee 15 way
137027-02 Shell for dee 15
130403 Servopot 6657S-1-103
Additional Items for /C and /C(M)
139096-01 Counter
139096-02 Connector
139096-03 Fixing plate
137013-04 Plug 5 pin molex
137013-18 Crimp
130200-41 Resistor 0.25W 2k2
130200-49 Resistor 0.25W 10k0
Additional Items for /Ma/r
138103 Motor(s), PF55-44C
138075-00 Micro sw CHERRY DC2
138075-01 Micro sw lever short
139089 Advance/rewind board
138004-05 Relay 24V DPCO
138004-02 Relay socket
137013-04 Housing 5MX 0.1
137013-18 Crimp
137017-09 Housing 10MX 0.156
137017-18 Crimp
138117-01 Switch centre off mom
138117-02 Switch lever black
138117-03 Switch bezel black
Additional Items for /V
137024 Plug free DIN
138026 Twin footswitch
Additional Items for Microtome Light
138034-02 Ballast 8W/115V
138034-01 Ballast 8W/230V
137032 Plug IEE straight
138017 Lamp assembly 2 x 4W
138018 Magnifier

Revised May 2012 18
5.3 Accessories and Consumables
Part Number Description
/R Conveyor for section ribbon.
50539 Orientating object holder, with fine adjustment screws, factory fitted.
50657 Orientating object holder, without fine adjustment screws, factory fitted.
51637 Quick release hardboard holder, without orientation, for 5040-02.
51637-1 Quick release hardboard holder, with orientation, for 5040-01.
50533 Quick release wax cassette holder, without orientation, for 5040-02.
50209 Triangular glass knife holder, fixed.
50681 Triangular glass knife holder, orientating. These accept 25mm high Latta-Hartmann knives with edges up to 15mm.
52985 CO2(gas) object holder and knife freezer.
50208 Sloped (20º) knife holder.
50213 Ralph knife holder.
50214 Ralph knife holder with section float-out trough. For long edged glass knives with edges up to 38mm.
52987 Illuminated magnifier.
50230 Standard knife with box, C-profile, 189 x 27 x 10mm. 22º angle, tapped to accept holder for Shandon knife
sharpener, safety cut-outs at each end.
50724 Knife sharpening back for 50230.
50232-1 hardened steel knife with box, C-profile, 160 x 35 x 9mm. 15º angle, suitable for large area sections.
50210 Knife sharpening back for 50232-1.
50207 Steel knife with box, C-profile, 160 x 32 x 13mm. 23º.
50211 Knife sharpening back for 50207.
51675 Handle for 50230, 50232-1 and 50207.
50234 Tungsten carbide tipped knife with box, 228 x 38 x 6mm. Suitable for cutting bone and hard plastics. NB: needs to be
resharpened at our factory.
52647 Stem for 50232-1 and 50234 knives.
52671 Knife sharpening kit. Includes hone, strop, oil, three grades of abrasive and instructions.
57612 Coarse abrasive, 6g net.
57611 Fine abrasive, 6g net.
57610 Honing compound, 6g net.
50239 Clock oil, 6g net.
50140 ‘Magnacut’ disposable blade system. Consists of magnetic blade holder, 50mm anti-roll plate, 10 blades, blade removal
tool, micro-adjusting T-piece and stem, box and instructions.
50133 ‘Magnacut’ disposable blade system. Consists of magnetic blade holder, Magnaplate anti-roll plate, 10 blades, blade
removal tool, box and instructions.
50540 ‘Magnacut’ disposable blade holder only.
52727 Long-life disposable blades for Magnacut, C-profile, pack of 50.
51564 ‘Magnacut’ blade removing tool.
50240 Feather blade holder with box.
50241 Feather disposable blades, pack of 50.
A KNIFE SHARPENING SERVICE IS AVAILABLE - DETAILS ON REQUEST
50260 5 - 300µm feed screw.
50259 3 - 180µm feed screw.
50258 2 - 120µm feed screw.
50257 1 - 60µm feed screw.
50255 0.5 - 30µm feed screw.
50256 0.2 - 12µm feed screw.
50259-1 3 - 180µm feed screw for /Ma/r option.
50258-1 2 - 120µm feed screw for /Ma/r option.
50257-1 1 - 60µm feed screw for /Ma/r option.
50255-1 0.5 - 30µm feed screw for Ma/r option.
50256-1 0.2 - 12µm feed screw for /Ma/r option.
50734-1 Object holder, 22mm diameter, solid.
50734-2 Object holder, 22mm diameter, solid - as 50734-1 but long type.
50735-1 Object holder, 37mm diameter, solid
50735-2 Object holder, 37mm diameter, solid - as 50735-1 but long type.
50744 Object holder, 5mm diameter, solid.
50721 Object holder, 50mm diameter.
50741 Object holder, 50 x 50mm.
50743 Object holder, 50 x 70mm.
50221 Quick release object holder clamp, complete with 50 metal object discs.
50690 Metal object holder discs, 22mm, pack of 50.
50204 Cork object holder discs, 22mm, pack of 50.
50204-1 Cork object holder discs, 50 x 50mm, pack of 50.
50162 Adaptor, to accept Ames cryostat, saddle type object holders.
52658 Vertical vice clamp.
57713-1 Bright Cryospray 134, 300ml aerosol can.
57713 Bright Cryospray 134, 300ml aerosol can, carton of 12 cans.
54791 Low temperature oil, 200ml bottle.
54791-1 Low temperature oil, 4.54 litre drum.
57344 Knife cleaning brush.
57808 Anti static brush, 12mm.
52531 Wax brush and debris tray.
53573 Spare fuse set for 5040-001 or 5040-002 with 225MR.
53574 Spare fuse set for 5040-001 or 5040-002 with 226MR.

Revised May 2012 19
MATERIAL SAFETY DATA SHEET
Name: CRYO-M-BED
Part Number: 53581
Product Information
By: Bright Instrument Company Limited
Address: St Margaret’s Way, Huntingdon, Cambs, PE29 6EU, England
Telephone: [phone] / 451 499 / 451 980 Emergency: 999
Fax: [phone]
Email: [e-mail]
Trade/Type: EMBEDDING COMPOUND
Container: Disposable plastic bottles
Uses: Embedding compound for frozen tissue
specimens
Data Sheet: 3
Description: Colourless viscous liquid
Information on Ingredients Physical and Chemical Properties
Blend of polyviol alcohol 217, thymol and water Colourless viscous liquid
Hazards Identification: Stability and Reactivity
Skin: Can cause skin irritation May react with oxidising materials
Respiratory: May cause difficulty in breathing if
exposed to very high concentration
Ingest: May be harmful by ingestion
Eyes: Eye irritation
First Aid procedures Toxicoloigical Information
Skin: Wash thoroughly, with soap and water No harmful effects if handled correctly. May give off toxic fumes
in the case of fire
Respiratory: Move to fresh air
Ingest: Rinse mouth out with water, in sever
cases seek medical attention
Eyes: Flush copiously for at least 15 minutes
Fire Fighting Measures Ecological Information
Hazards: May cause toxic fumes Degradable, miscible in all proportions
Equipment: Water spray, foam, dry powder, Co2
Accidental Release Measures Disposal Considerations
Spill: Absorb on an inert absorbent, bag and
arrange disposal. Wash area in water
and detergent
Waste: Bag and dispose of in accordance with
local authority requirements
Handling and Storage Transport Information
Special
Requirements:
NONE No restrictions
Exposure Controls Regulatory Information
OES: Not assigned (long term, 8 hour TWA) NONE
Skin: Avoid contact
Respiratory: Avoid very high concentrations
Ingest: Do not eat, drink or smoke
Eyes: Goggles should be worn
Additional Information/Comments:
Information given is, to the best of the Company’s knowledge and belief, accurate and reliable. However, no warranty, guarantee or representation is made
to it’s accuracy, reliability of completeness.
Issue 6 /June 2000 - Last reviewed: June 2004 /Next review: June 2006

Revised May 2012 20
MATERIAL SAFETY DATA SHEET
Name: CRYOSPRAY
Part Number: 57713
Product Information
By: Bright Instrument Company Limited
Address: St Margaret’s Way, Huntingdon, Cambs, PE29 6EU, England
Telephone: [phone] / 451 499 / 451 980 Emergency: 999
Fax: [phone] Email: [e-mail]
Trade/Type: Bright Cryospray 134 Aerosol Freezing Agent
Container: Aerosol
Uses: Rapid Freezing of tissue specimens to –52ºC
Data Sheet: 48
Description: Colourless viscous liquid
Information on Ingredients Physical and Chemical Properties
1, 1, 1, 2 – Tetrafluoroethane – contents 80-100% Appearance: Aerosol
Odour: Characteristic
Hazards Identification: Stability and Reactivity
May cause frost bite if intentionally misused Avoid powdered metal, alkali metals alkali earth metals
Avoid heat, flames and other sources of ignition
First Aid procedures Toxicoloigical Information
Skin: Wash thoroughly, with soap and water May cause frost bite if intentionally misused No harmful effect if
handled correctly. May give off toxic fumes in the case of fire
Respiratory: Provide rest, warmth and fresh air If
discomfort continues, seek medical
attention
Ingest: Rinse mouth out with water, in sever
cases seek medical attention
Eyes: Flush copiously for at least 15 minutes
SEEK MEDICAL ADVICE
Fire Fighting Measures Ecological Information
This product is not classed as flammable under current
regulations. Special
Fire Fighting Procedures : use water to keep fire exposed
containers cool and disperse vapours. Breathing apparatus
should be worn if exposure of fumes s likely.
Unusual Fire Explosion Hazards: Possible risk of can rupture
when exposed to fire/high temperatures.
Hazardous Decomposition Products: Fire or high
temperatures create halogenated hydrocarbons, oxides of
carbon
Water Hazard Classification: Discharge of product will enter the
atmosphere and will not result I long term aqueous
contamination
Global Warming Potential: 0.28 (R11=1) (1,1,1,2 –
Tetrafluorethane) Ozone Depletion Potential 0 (R11=1)
(1,1,1,2 – Tetrafluorethane)
Accidental Release Measures Disposal Considerations
Spill: Let evaporate and ventilate area well Waste: DO NOT pierce or burn empty cans.
Dispose of in accordance with local
authority requirements.
Handling and Storage Transport Information
Usage Precautions: CAUTION pressurised container DO NOT
expose to temperatures exceeding 50ºC
DO NOT puncture or incinerate even when empty
DO NOT spray onto naked flame or any incandescent material
Spray in short bursts to prevent cooling of the can
STORAGE PRECAUTIONS: Store in a cool dry place, away
from all sources of heat, including direct sunlight
Road: UN No.1950 CEFUC TEC (R) No.20G26-1 ADR
Class 2 ADR ITEM No.5A
Air: UN Air No.1950 Air Transport Class 2
Sea: UN Sea No.1950 Sea Transport Class No.2 IMDG
Page No.2102
Exposure Controls Regulatory Information
1,1,1,2 – Tetrafluoroethane (HFC 134a) OED: Long term exp
(8hours TWA ref period) 1000ppm (rec)/4240mg/m3 Skin: Avoid
contact, it is advised to wear gloves
Respiratory: Good ventilation required if used in confined space
Ingest: Do not eat, drink or smoke
Eyes: Wear goggles during use if there is any risk of eye contact,
but not generally required under normal use.
CHIP: S23 DO NOT breathe gas/fumes/vapour/spray
CHIP: S24/S25 Avoid contact with skin and eyes
CHIP: S51 use only in well ventilated areas
COSHH Regulations 1999
Additional Information/Comments:
Information given is, to the best of the Company’s knowledge and belief, accurate and reliable. However, no warranty, guarantee or representation is made
to it’s accuracy, reliability of completeness.
Issue 7 /January 2004 - Last reviewed: May 2004 /Next review: May 2006