Buchi SpeedExtractor E-916 User manual

SpeedExtractor E-916 / E-916XL / E-914
Operation Manual
093218G en

Imprint
Product Identification:
Operation Manual (Original), SpeedExtractor E-916 / E-916XL / E-914
093218G en
Publication date: 07.2019
BÜCHI Labortechnik AG
Meierseggstrasse 40
Postfach
CH-9230 Flawil 1
E-Mail: [e-mail]
BUCHI reserves the right to make changes to the manual as deemed necessary in the light of experience; espe-
cially in respect to structure, illustrations and technical detail.
This manual is copyright. Information from it may not be reproduced, distributed, or used for competitive purpos-
es, nor made available to third parties. The manufacture of any component with the aid of this manual without
prior written agreement is also prohibited.

Table of contents
2SpeedExtractor E-916 / E-916XL / E-914 Operation Manual
Table of contents
1 About this manual.............................................................................................................................................................. 5
1.1 Reference documents ......................................................................................................................................... 5
1.2 Trademarks.............................................................................................................................................................. 5
1.3 Abbreviations......................................................................................................................................................... 6
2 Safety ...................................................................................................................................................................................... 7
2.1 User qualication.................................................................................................................................................. 7
2.2 Proper use................................................................................................................................................................ 7
2.3 Improper use .......................................................................................................................................................... 7
2.4 Safety warnings and safety signs used in this manual............................................................................ 8
2.5 Product safety ......................................................................................................................................................10
2.5.1 General hazards.....................................................................................................................................10
2.5.2 Warning labels on housing and assemblies ................................................................................11
2.5.3 Personal protective equipment .......................................................................................................11
2.5.4 Built-in safety elements and measures .........................................................................................12
2.6 General safety rules............................................................................................................................................13
3 Technical data.................................................................................................................................................................... 15
3.1 Scope of delivery.................................................................................................................................................15
3.2 Instrument congurations...............................................................................................................................15
3.3 Materials used......................................................................................................................................................18
3.4 Technical data overview...................................................................................................................................18
4 Description of function ..................................................................................................................................................21
4.1 Functional principle ...........................................................................................................................................21
4.2 Overview of the instrument............................................................................................................................21
4.3 Overview of the extraction process .............................................................................................................23
4.4 Schematic representation of the process ..................................................................................................24
4.5 Controls and connections................................................................................................................................25
4.5.1 Instrument controls..............................................................................................................................25
4.5.2 Main displays of the instrument......................................................................................................26
4.5.3 General information on buttons......................................................................................................29
4.5.4 Rear connections...................................................................................................................................30
4.5.5 Side connections...................................................................................................................................30
5 Putting into operation.................................................................................................................................................... 31
5.1 Installation site.....................................................................................................................................................31
5.2 Electrical connections .......................................................................................................................................32
5.3 Gas and solvent connections..........................................................................................................................33
5.4 Dehydration..........................................................................................................................................................34
5.5 Functional test .....................................................................................................................................................34

Table of contents
3SpeedExtractor E-916 / E-916XL / E-914 Operation Manual
6 Operation ............................................................................................................................................................................35
6.1 Method development.......................................................................................................................................35
6.2 Preparing the instrument ................................................................................................................................36
6.2.1 Solvent reservoir....................................................................................................................................36
6.2.2 Modifying the SOLVENT LIST ............................................................................................................37
6.2.3 Preheating the instrument ................................................................................................................38
6.2.4 Activating/deactivating positions...................................................................................................39
6.2.5 Leak test....................................................................................................................................................40
6.2.6 Flushing the instrument.....................................................................................................................43
6.2.7 Activating the EcoMode .....................................................................................................................44
6.3 Preparing the sample ........................................................................................................................................45
6.3.1 Sample preparation..............................................................................................................................45
6.3.2 Extraction cell selection......................................................................................................................46
6.3.3 Packing of the cells...............................................................................................................................46
6.4 Extraction process ..............................................................................................................................................49
6.4.1 Placing the cells and bottles/vials into the instrument...........................................................49
6.4.2 Stages of an extraction cycle ............................................................................................................50
6.4.3 Creating new methods........................................................................................................................51
6.4.4 Summary of operating parameters ................................................................................................54
6.4.5 Example of an extraction method ..................................................................................................56
6.4.6 Open an existing method ..................................................................................................................56
6.4.7 Optimize a process ...............................................................................................................................57
6.4.8 Start, pause, stop and abort extraction.........................................................................................58
6.4.9 Post-extraction procedures...............................................................................................................58
6.5 Creating a report (optional) ............................................................................................................................59
7 Maintenance.......................................................................................................................................................................61
7.1 Daily maintenance..............................................................................................................................................61
7.2 Periodic maintenance .......................................................................................................................................62
7.2.1 Sealing system........................................................................................................................................62
7.2.2 Replacing the cup seals ......................................................................................................................62
7.2.3 Tube connections and needles........................................................................................................63
7.2.4 Septum......................................................................................................................................................65
7.3 Pump maintenance............................................................................................................................................66
7.3.1 Connections............................................................................................................................................66
7.3.2 Back washing..........................................................................................................................................67
8 Troubleshooting................................................................................................................................................................69
8.1 Malfunctions and their remedy .....................................................................................................................69
8.1.1 Action in case of a re..........................................................................................................................69
8.1.2 General malfunctions and their remedy.......................................................................................69
8.1.3 Handling and resolving blockages .................................................................................................74
8.1.4 The pump is not aspirating properly .............................................................................................79
8.1.5 Replacement of check valves............................................................................................................80
8.1.6 Precipitation in the outlet lines........................................................................................................81
8.1.7 Malfunctions of the rotating valves................................................................................................82
8.1.8 Upgrading a 2-port mixer to a 4-port mixer................................................................................82

Table of contents
4SpeedExtractor E-916 / E-916XL / E-914 Operation Manual
Read this manual carefully before installing and running your system and note the safety precautions in chapter 2
in particular. Store the manual in the immediate vicinity of the instrument, so that it can be consulted at any time.
No technical modications may be made to the instrument without the prior written agreement of BUCHI. Unau-
thorized modications may aect the system safety or result in accidents.
This manual is copyright. Information from it may not be reproduced, distributed, or used for competitive pur-
poses, nor made available to third parties. The manufacture of any component with the aid of this manual without
prior written agreement is also prohibited.
The English manual is the original language version and serves as basis for all translations into other
languages. If you need another language version of this manual, you can download available versions
at www.buchi.com.
8.2 Description of the service menu...................................................................................................................83
8.2.1 Checking the valves .............................................................................................................................84
8.2.2 Checking the sensors...........................................................................................................................85
8.2.3 Running the pump ...............................................................................................................................85
8.2.4 Inspecting the lines (ow test).........................................................................................................86
8.2.5 Moving the cell and vial lift ...............................................................................................................88
8.2.6 Changing the fan performance........................................................................................................88
8.2.7 Displaying the operating hours.......................................................................................................88
8.2.8 Unit information....................................................................................................................................88
8.3 Customer service.................................................................................................................................................89
9 Shutdown, storage, transport and disposal............................................................................................................91
9.1 Storage and transport.......................................................................................................................................91
9.2 Disposal..................................................................................................................................................................92
9.3 Health and safety clearance............................................................................................................................93
10 Spare parts..........................................................................................................................................................................95
11 Declarations and requirements.................................................................................................................................105
11.1 FCC requirements (for USA and Canada) ................................................................................................ 105

1 About this manual
5SpeedExtractor E-916 / E-916XL / E-914 Operation Manual
1 About this manual
This manual describes the SpeedExtractor E-916 / E-914 and provides all information required for
its safe operation and to maintain it in good working order. It is addressed in particular to laboratory
personnel and operators.
NOTE
The symbols pertaining to safety are explained in chapter2.
1.1 Reference documents
For information on complementary BUCHI devices, please refer to the corresponding manuals:
Complementary devices
MultivaporP-6/P-12,OperationManual
VacuumController,Operation Manual
Vacuum Pump, Operation Manual
Syncore Platform, Operation Manual
SyncoreAccessories,OperationManual
NOTE
• All manuals are available via www.buchi.com
• For download a free online registration is required
1.2 Trademarks
The following product names and any registered and unregistered trademarks mentioned in this
manual are used for identication purposes only and remain the exclusive property of their respective
owners:
• SpeedExtractor is a registered trademark of BÜCHI Labortechnik AG
• ASE is a registered trademark of Dionex Corporation

1 About this manual
6SpeedExtractor E-916 / E-916XL / E-914 Operation Manual
1.3 Abbreviations
Process-related
ASE Accelerated Solvent Extraction
PSE Pressurized Solvent Extraction
Materials and chemicals
FEP Combination of tetrauoroethylene and hexauoropropylene
FFPM Peruoro caoutchouc
PTFE Polytetrauoroethylene
POM Polyoxymethylene (commercialized as Delrin® by DuPont)
PEEK Polyether ether ketone
THF Tetrahydrofurane
Miscellaneous
FW rm ware
qty quantity
DT Temperature dierence
Dp Pressure dierence

2 Safety
7SpeedExtractor E-916 / E-916XL / E-914 Operation Manual
2 Safety
This chapter highlights the safety concept of the SpeedExtractor and contains general rules of behav-
ior and warnings about hazards concerning the use of the product.
The safety of users and personnel can only be ensured if these safety instructions and the safety re-
lated warnings in the individual chapters are strictly observed and followed, therefore, the manual must
always be available to all persons performing the tasks described herein.
2.1 User qualication
The instrument may be used only by laboratory personnel or other persons who on account of instruc-
tion or professional experience have an overview of the dangers that can develop when operating the
instrument.
Personnel without such instruction or persons who are currently being trained require careful supervision.
The present Operation Manual serves as a basis for instruction.
2.2 Proper use
The instrument has been designed and built for laboratory use only. It serves for activities associated
with the parallel extraction of multiple samples by means of heating under pressure. The pressure is
typically applied by the HPLC pump.
2.3 Improper use
Applications beyond those described above are improper. Furthermore, applications that do not
comply with the technical data are also considered improper. The operator bears the sole risk for any
damage caused by such improper use.
The following applications are expressly forbidden:
• Use of solvents with an self ignition point between 40 to 220°C.
• Use of the instrument in rooms that require ex-protected instruments.
• Use as a calibrating instrument for other instruments.
• Preparation of samples that can explode or iname due to shock, friction, heat, or spark formation.
• Use in high pressure situations, i.e. > 200bar.
• Use in conjunction with solvents that have a low self ignition point or contain peroxides, such as
diethyl ether or THF.
• Use of cells, seals, hoses, and tubes other than the originals from BUCHI.

2 Safety
8SpeedExtractor E-916 / E-916XL / E-914 Operation Manual
2.4 Safety warnings and safety signs used in this manual
DANGER, WARNING, CAUTION and NOTICE are standardized signal words for identifying levels of
hazard seriousness of risks related to personal injury and property damage. All signal words, which are
related to personal injury are accompanied by the general safety sign.
For your safety it is important to read and fully understand the table below with the dierent signal
words and their denitions!
Sign Signal word Definition Risk level
!DANGER Indicatesahazardoussituationwhich,ifnotavoided,willresultin
death or serious injury. ★★★★
!WARNING Indicatesahazardoussituationwhich,ifnotavoided,couldresult
in death or serious injury. ★★★☆
!CAUTION Indicatesahazardoussituationwhich,ifnotavoided,mayresult
in minor or moderate injury. ★★☆☆
no NOTICE Indicates possible property damage, but no
practices related to personal injury.
★☆☆☆
(propertydamageonly)
Supplementary safety information symbols may be placed in a rectangular panel on the left to the
signal word and the supplementary text (see below example).
Space for
supplementary
safety
information
symbols.
!SIGNAL WORD
Supplementary text, describing the kind and level of hazard/risk seriousness.
• List of measures to avoid the herein described, hazard or hazardous situation.
• ...
• ...
Table of supplementary safety information symbols
The reference list below incorporates all safety information symbols used in this manual and their
meaning.
Symbol Meaning
General warning
Electrical hazard
Harmful to life-forms

2 Safety
9SpeedExtractor E-916 / E-916XL / E-914 Operation Manual
Symbol Meaning
Fire hazard
Hot item, hot surface
Device damage
Inhalation of substances
Chemical burns by corrosives
Wear laboratory coat
Wear protective goggles
Wear protective gloves
Heavy weight, lifting requires more than one person
Additional user information
Paragraphs starting with Note transport helpful information for working with the device/software or its
supplementary. Notes are not related to any kind of hazard or damage.
NOTE
Useful tips for the easy operation of the instrument/software.

2 Safety
10 SpeedExtractor E-916 / E-916XL / E-914 Operation Manual
2.5 Product safety
The SpeedExtractor has been designed and built in accordance with current state-of-the-art technol-
ogy. Safety warnings in this manual (as described in section 2.4) serve to make the user alert to and
avoid hazardous situations emanating from residual dangers by giving appropriate counter measures.
However, risks to users, property and the environment can arise when the instrument is damaged,
used carelessly or improperly.
2.5.1 General hazards
The following safety messages show hazards of general kind which may occur when handling the
instrument. The user shall observe all listed counter measures in order to achieve and maintain the
lowest possible level of hazard.
Additional warning messages can be found whenever actions and situations described in this manual
are related to situational hazards.
!WARNING
Death or serious injuries by formation of explosive atmospheres inside the instrument.
• Before operation, check all gas connections for correct installation
• Regularly discharge the waste bottle to avoid overow
• Check for proper system tightness
!DANGER
Death or serious injuries by use in explosive environments.
• Do not store or operate the instrument in explosive environments
• Provide sucient ventilation and make sure to directly withdraw fumes
!WARNING
Death or serious burns by ammable vapors.
• Remove all sources of ammable vapors
• Do not store ammable chemicals in the vicinity of the device
!CAUTION
Risk of burns by hot heating block and extraction cells.
• Do not touch hot parts or surfaces
• Let the system and inserted extraction cells cool down safely
• Do not move the instrument or parts of it when hot

2 Safety
11 SpeedExtractor E-916 / E-916XL / E-914 Operation Manual
NOTICE
Risk of instrument damage by liquids or mechanical shocks.
• Do not spill liquids over the instrument or its components
• Do not move the instrument when it is loaded with sample liquid
• Do not drop the instrument or its components
• Keep external vibrations away from the instrument
• Safely attach the instrument to the bench in earthquake prone regions
• Do not operate the instrument without the safety shield installed
NOTICE
Risk of instrument damage by wrong mains supply.
• External mains supply must meet the voltage given on the type plate
• Check for sucient grounding
2.5.2 Warning labels on housing and assemblies
The following warning sticker(s) can be found on the housing or assemblies of the SpeedExtractor:
Symbol Meaning Location
Hot item, hot surface Sticker/label, located at the heating block
2.5.3 Personal protective equipment
Always wear personal protective equipment such as protective eye goggles, protective clothing and
gloves. The personal protective equipment must meet all requirements of the supplementary data
sheets for the chemicals used.
!WARNING
Serious chemical burns by corrosives.
• Observe supplementary data sheets of all used chemicals.
• Handle corrosives in well ventilated environments only.
• Always wear protective goggles.
• Always wear protective gloves.
• Always wear protective clothes.
• Do not use damaged glassware.

2 Safety
12 SpeedExtractor E-916 / E-916XL / E-914 Operation Manual
2.5.4 Built-in safety elements and measures
• The heating element is equipped with overtemperature protection which is activated at
260°C±10°C.
• The pressure parts are protected by a mechanical pressure control valve which is activated at
200bar±20bar.
• To start a program at least one extraction position must be activated.
• Safety shield sensor: To start an extraction the protection shield must be closed.
• Vial rack sensor: To start an extraction the vial rack must be installed.
• The presence of extraction cells in the heating block is checked in the tightness test at the begin-
ning of each extraction process.
Seismic tie-down
In earthquake-susceptible regions the instrument
should be tied down by the ventilation slot on the
rear of the instrument.

2 Safety
13 SpeedExtractor E-916 / E-916XL / E-914 Operation Manual
2.6 General safety rules
Responsibility of the operator
The head of the laboratory is responsible for training his/her personnel.
The operator shall inform the manufacturer without delay of any safety-related incidents which might
occur during operation of the instrument or its accessories. Legal regulations, such as local, state and
federal laws applying to the instrument or its accessories must be strictly followed.
Duty of maintenance and care
The operator is responsible for the proper condition of instrument. This includes maintenance, service
and repair jobs that are performed on schedule by authorized personnel only.
Spare parts to be used
Use only genuine consumables and spare parts for maintenance to assure good system performance,
reliability and safety. Any modications of spare parts or assemblies are only allowed with the prior
written permission of the manufacturer.
Modications
Modications to the instrument are only permitted after prior consultation and with the written approval
of the manufacturer. Modications and upgrades shall only be carried out by an authorized BUCHI
technical engineer. The manufacturer will decline any claim resulting from unauthorized modications.

2 Safety
14 SpeedExtractor E-916 / E-916XL / E-914 Operation Manual

3 Technical data
15 SpeedExtractor E-916 / E-916XL / E-914 Operation Manual
3 Technical data
This chapter introduces the reader to the SpeedExtractor and its main components. It contains techni-
cal data, requirements and performance data.
3.1 Scope of delivery
Check the scope of delivery according to the order number and your shipping note.
NOTE
For detailed information on the listed products, see www.buchi.com or contact your local dealer.
3.2 Instrument congurations
The SpeedExtractor is available in 21 dierent congurations diering in the number of positions (E-916:
6 positions, E-916XL: 6 position, E-914: 4 positions), the type of solvent mixer (2 ports or 4 ports), and the
size of the extraction cells (E-916: 10– 40mL, E-916XL: 60 mL, E-914: 10 – 120mL). The SpeedExtractor
E-914 is available with and without pedestal (the pedestal allows to accommodate large volume collec-
tion recipients).
E-914 without and with pedestal

3 Technical data
16 SpeedExtractor E-916 / E-916XL / E-914 Operation Manual
List of loose parts
Position Item PU Universal Order no.
E-914
Order no.
E-916
Order no.
E-916XL
aFEP tube D3.2/1.6, 5m 2 11055604
bFEP waste tube 1/16", 0.5m 4/6 053303
cSolvent bottle 1 L 1 053203
dQuartz sand 0.3–0.9mm, 2.5kg 1 037689
eExtraction Record demo license 1 053074
fExtraction cell carrier 1 053691 053690 110 69547
gSolvent lter 4 044340
hTurix wrench 1 044349
Number of positions
1 1 5 1
Size of extraction cells
Number of solvent ports
E-914: 4 positions
4
E-914: 4 positions mounted on pedestal*
5
E-916: 6 positions
6
10 mL (E-916 only)
01
20 mL (E-916 only)
02
40 mL (E-916 or E-914 only)
04
80 mL (E-914 only)
08
120 mL (E-914 only)
12
2-port
2
4-port (E-916 and E-914 only)
4
60 mL (E-916XL only)
06
ab
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3 Technical data
17 SpeedExtractor E-916 / E-916XL / E-914 Operation Manual
List of loose parts
Position Item PU Universal Order no.
E-914
Order no.
E-916
Order no.
E-916XL
iAllen wrench 3mm 1 000610
jSpanner wrench 1/4" 1 053204
kSpanner wrench 8/10mm 1 053608
lTorx screwdriver TX20 1 053668
mUSB cable 2.0 A-B, 4.5m 1 049226
nPlug screws 2 053209
oMetal frit 25 049568
pCup seals, top 12 053671 053669 11069763
pCup seals, bottom 12 053670
qCellulose lter, top 100 051249 049572 11069533
qGlass ber lter, bottom 100 11055932
rSwagelok nut and ferrules 1/8" 111055342
sUNF-28 Fitting 1/8", green 10 053663
sUNF-28 Ferrule 1/8", green 10 053664
tUNF-28 Fitting 1/16", gray 25 044816
tUNF-28 Ferrule 1/16", gray 25 044269
Supporting ring PEEK 2 053667 053666 11069769
Filter hook 1 053316
Plunger 1 053038 053037 110 69530
Syringe 60mL 1 034882
Brush small 1 053256
Brush large 1 053257
Gripper extraction cell 1 053026 053030 110 69534
Bit wrench 1 052783
Extruder rod 111055284
ab
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3 Technical data
18 SpeedExtractor E-916 / E-916XL / E-914 Operation Manual
List of loose parts
Position Item PU Universal Order no.
E-914
Order no.
E-916
Order no.
E-916XL
Tube cutter 1019830
3.3 Materials used
Materials used
Component Material designation
Housing SpeedExtractor Stainless steel
Lines to pump FEP
Solvent valve PEEK, FFPM
Mixer PEEK, FFPM
Media valve PEEK, PTFE
Lines to and from heating block Stainless steel
Pressure gauges Stainless steel
Position valves Stainless steel, PTFE
Outlet valves PEEK, PTFE
Heating block Aluminum
Heating block cover PTFE
Cup seals PTFE
Extraction cells Stainless steel
Lines to waste FEP
Needles Stainless steel
Collection vials Glass
Septa for vials Silicon, PTFE
Collection unit Stainless steel, POM
Pump PTFE, ceramic, stainless steel
Protective shield Glass, POM
ab
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3 Technical data
19 SpeedExtractor E-916 / E-916XL / E-914 Operation Manual
3.4 Technical data overview
Technical data of the SpeedExtractor
Description Technical data
Dimensions (W×H×D) 670×725×500mm
Weight 90kg
Connection voltage 100– 240VAC ±10%
Max. power consumption max. 1750W
Mains connection 3-pole (P, N, E) via power cord
Frequency 50/60Hz
Fuse 14 A/240V
Interface USB 2.0
Installation category II
Degree of protection IP21
Pollution degree 2
Temperature control range 30–200°C
Temperature accuracy ±3°C
Pressure range 50–150bar
Pressure accuracy ±5bar
Primary pressure nitrogen connection 6– 10bar
Flow rate pump 1– 50mL/min
Precision ow rate ±2%
Precision mixer ±2% (±5% for isopropanol)
Extraction cell size E-916: 10, 20, 40mL
E-916XL: 60 mL
E-914: 10*, 20*, 40, 80, 120mL
Environmental conditions For indoor use only
Temperature 5–40°C
Altitude up to 2000m
Humidity maximum relative humidity 80% for temperatures
up to 31°C, and then linearly decreasing to 50%
at 40°C
Noise level <70dB
*Accessories