Covaris AFA-TUBE 520292 User manual

1Part Number: 010503 Rev C
Covaris
October 2020
Intended Use
The product is designed for AFA-energecs™ sample processing in the following Covaris AFA®
Focused-ultrasonicators: ME220, LE220-plus, LE220R-plus, and LE220RSc.
For current protocols: www.covaris.com/technical-resources/
Operang Limits
Ordering Informaon
Plate Denions
Notes
• This strip must be used with the Rack 8 AFA-TUBE TPX strip PN 500609 or PN 500685
• Designed for use with automated 1, 8, and 96 channel pipeors
• Recommended instrucons are subject to change without noce
• If Plate Denion is not in SonoLab™ contact Technical Support at [e-mail]
8 AFA-TUBE™ TPX Strip (PN 520292)
LE220-plus, LE220R-plus, LE220Rsc, and R230 -
Average Incident Power (AIP) (Max)
90 W connuous mode
250 W pulsing mode
ME220 - Average Incident Power (AIP) (Max) 9 W connuous mode
14 W pulsing mode
Volume (Max) 200 µl
Water Level Automacally adjusted
Centrifuge 2,200 x RCF maximum
Storage Dry at ambient temperature and protected from direct sunlight
Part Name Part Number Part Descripon
8 AFA-TUBE TPX Strip (12) 520292 8 well AFA-TUBE TPX Strip
Rack 8 AFA-TUBE TPX Strip 500685 LE220-plus, LE220R-plus, and LE220Rsc Rack
Rack 8 AFA-TUBE TPX Strip 500609 ME220 Rack
Thin Foil Seals (25) 520235 Foil seals for 12 Strip Plate
Strip Foil Seal (12) 520108 Foil seals for sealing single strips during AFA processing
8 AFA-TUBE TPX Strip Caps 500639 Caps for sealing during AFA processing
Instrument(s) Part Descripon
ME220 “8 AFA-TUBE TPX Strip PN 520292”
LE220-plus, LE220R-plus, and LE220Rsc “LE220plus_500685 8 AFA-TUBE TPX Strip 1.8 oset”

2Part Number: 010503 Rev C
Covaris
October 2020
Package Contents
• Ready-to-use 8 AFA-TUBE TPX Strip
• Desiccant pouch
Nominal Strip Dimensions
Instrucons for Use
The 8 AFA-TUBE TPX Strip is a ready-to-use sample processing vessel. The specially designed 8 Strip is opmized for use with Covaris
Adapve Focused Acouscs® (AFA®).
The strip is designed as typical labware compable with automaon including heang blocks, magnec stands, etc. If volume is at
maximum, be sure to pipee slowly from the top of the tube to ensure no sample displacement.
Overall Height (top of tube) 19.1 mm (without seal)
Tu b e c e nt e r - t o - c e n t e r s p a c i n g 9.0 mm (SBS standard paern)
Tube depth 18.6 mm
Interior clearance diameter 5.2 mm (maximum p diameter 18.5 mm from end)
CAUTION: Remove the desiccant pack prior to use.

3Part Number: 010503 Rev C
Covaris
October 2020
Document Part # Revision Date Descripon of Change
010503 A05/2019 Release of 8 AFA-TUBE TPX Strip
010503 B 02/2020 Updang the PN for the 8 AFA-TUBE TPX Strip
010503 C 10/2020 Update document formang and hyperlinks
Recommended Sequence for Use
1. Fill the tubes:
• Dispense the samples about 2 to 3 mm from the boom of the tube or at the boom depending on sample volume.
2. Seal the plate for AFA-processing:
• ME220: Apply strip caps or foil seal strip to tops of tubes.
• LE220-plus, LE220R-plus, LE220Rsc: Apply strip caps or thin foil seal to tops of tubes. If using strip caps, use the solid rack
lid labeled “FOR CAP STRIP USE”. If using thin foil to seal, use the open rack lid labeled “FOR FOIL SEAL USE”.
3. Pre AFA-Centrifugaon:
• Centrifuge the strip aer sample addion to ensure the sample is at the boom of the tube for ≤ 10 seconds at up to
2,200 RCF. Visually inspect to verify that all liquid is at the boom of each tube before proceeding to Step 4.
4. AFA-processing (examples):
• ME220: Refer the Quick Guide, DNA shearing with ME220 Focused-ultrasonicator (covaris.com/wp/wp-content/
uploads/resources_pdf/pn_010349.pdf). The strip must be in the ME220 Rack 8 AFA-TUBE TPX Strip (PN 500609) using
the waveguide (PN 500526) for processing.
• LE220-plus, LE220R-plus, LE220Rsc: Refer to the Quick Guide, DNA shearing with Focused-ultrasonicator (covaris.
com/wp/wp-content/uploads/2020/06/pn_010433.pdf). The strips must be in the Rack 8 AFA-TUBE TPX Strip for
LE220-plus (PN 500685) for processing.
5. Post AFA Centrifugaon:
• Centrifuge the strip aer AFA for ≤ 10 seconds at up to 2,200 RCF before removing any sample.
6. Downstream sample handling:
• Aer AFA treatment, the samples are ready for downstream processing. The strip caps or foil seal can be removed for
processing in the 8 AFA-TUBE TPX Strip. Do not use the strip for long-term storage of the samples.
Technical Assistance
Ongoing assistance with the operaon or applicaon of the equipment and/or troubleshoong is provided via:
• Telephone
- United States: Tel: [phone] during the hours of 9:00 a.m. to 5:00 p.m., Monday through Friday,
Eastern Standard Time (EST), Greenwich Mean Time (GMT-05:00)
- Europe: Tel: [phone], during the hours of 9:00 a.m. to 5:00 p.m., Monday through Friday, Greenwich Mean Time
• E-mail queries to [email protected] or applica[e-mail]
Revision History

4Part Number: 010503 Rev C
Covaris
October 2020
Appendix A: Removing or Installing the Intensier (Covaris PN 500141) from a Covaris E System
The 500141 Intensier is a small inverted stainless steel cone centered over the E-Series transducer by four stainless wires. The wires
are held in place by a black plasc ring pressed into the transducer well.
If an AFA protocol requires “no Intensier”, please remove the Intensier, using the following steps:
1. Empty the water bath. Start the instrument and start the SonoLab™ soware.
2. Wait for the homing sequence to complete (the transducer will be lowered with the rack holder at the home posion, allowing
easy access to the Intensier).
3. Grasp opposite sides of plasc ring and gently pull the enre assembly out of the transducer well. Do not pull on the steel cone
or the wires. The ring is a fricon t in the well – no hardware is used to hold it in place.
The 500141 Intensier (le) shown installed in the E-Series transducer well and (right) removed. Note the “UP” marking at the center
of the Intensier.
If a protocol requires the Intensier to be present, simply reverse this process:
4. Align the black plasc ring with the perimeter of the transducer well. Note that the at side of the center cone (marked UP)
should be facing up (away from the transducer).
5. Gently press each secon of the ring into the well unl the ring is seated uniformly in contact with the transducer, with
approximately 2 mm of the ring evenly exposed above the transducer assembly. Do not press on the cone or wires. The
rotaon of the ring relave to the transducer assembly is not important.
6. Rell the tank. Degas and chill the water before proceeding.
Technical Support – Ongoing assistance with the operaon or applicaon of the equipment and/or troubleshoong is provided via:
• Telephone
- United States: Tel: [phone] during the hours of 9:00 a.m. to 5:00 p.m., Monday through Friday,
Eastern Standard Time (EST), Greenwich Mean Time (GMT-05:00)
- Europe: Tel: [phone], during the hours of 9:00 a.m. to 5:00 p.m., Monday through Friday, Greenwich Mean Time
• E-mail queries to [email protected] or applica[e-mail]