Covaris microTUBE-500 User manual

1Part Number: 010526 Rev A
Covaris
June 2020
Introducon
The microTUBE-500 AFA Fiber Screw-Cap (PN 520185) is a singular vessel designed to hold up to 500 µl of
sample for sample processing. Each tube contains an AFA ber that improves reproducibility, promotes an
isothermal process, reduces fragmenng mes, and allows larger sample volumes to be processed.
The microTUBE-500s have been validated for DNA shearing at volumes of 320 µl and 500 µl. They are
compable with the M-series, S220, E-series and L-Series Covaris instruments and require the use of an
instrument specic Rack or Holder. Refer to the Covaris-cered Consumable Guide for further informaon
(covaris.com/wp-content/uploads/M020065.pdf). DNA shearing protocols with the microTUBE-500 are also
instrument specic. Do not store sheared samples in the microTUBEs.
The microTUBE-500 has a 2D barcode which can be used for sample traceability.
The microTUBE-500s are also a part of the truXTRAC® FFPE Plus product line and included in the following kits: PN 520255, PN
520252, PN 520262, and PN 520271. Please refer to the Nucleic Acid Extracon from FFPE Protocols: (covaris.com/resources/
protocols/nucleic-acid-extracon-from-pe/).
Recommended instrucons are subject to change without noce.
Ordering Informaon
• microTUBE-500 AFA Fiber Screw-Cap (25) (PN 520186)
• microTUBE-500 Screw-Cap Prep Staon (PN 500510)
• microTUBE-500 Centrifuge and Heat Block Adapter (10) (PN 500503)
DNA Shearing Protocols
• Quick Guide: DNA Shearing with M220 (hps://covaris.com/wp-content/uploads/pn_010252.pdf)
• Quick Guide: DNA Shearing with ME220 (hps://covaris.com/wp-content/uploads/pn_010349.pdf)
• Quick Guide: DNA Shearing with S220 (hps://covaris.com/wp-content/uploads/pn_010368.pdf)
• Quick Guide: DNA Shearing with E220 (www.covaris.com/wp-content/uploads/pn_010308.pdf)
• Quick Guide: DNA Shearing with LE220 (www.covaris.com/wp-content/uploads/pn_010156.pdf)
• Quick Guide: DNA Shearing with LE220-plus/R-plus/Rsc (www.covaris.com/wp-content/uploads/pn_010433.pdf)
Product Insert: microTUBE-500 AFA Fiber Screw-Cap

2Part Number: 010526 Rev A
Covaris
June 2020
Operang Limits and Condions
M220
ME220
S220
E220evoluon
Temperature (water bath) 4 °C minimum; 25 °C maximum
Recommended Sample Volume 500 µl, +/- 10 µl or 320 µl, +/- 10 µl
Centrifuge Up to 5,000 x g for 15 min with centrifuge adapters PN 500503
Storage Room temperature (15 °C to 30 °C)
Peak Incident Power 75 W maximum
Duty Cycle 25% maximum
Holder + Insert Holder XTU PN 500414; Insert XTU PN 500471
Peak Incident Power 75 W maximum
Duty Cycle 20% maximum
Rack Rack 4-Place microTUBE-500 (500525)
Waveguide ME220 Waveguide 4 Place (500534)
Plate Denion 4 microTUBE-500 Screw-Cap PN 520185
Peak Incident Power 200 W maximum*
Duty Factor 20% maximum
Holder Holder PN 500449
Water level (RUN scale) Level 8
*The S220 can operate using a power combina on of 200W with 10% Duty Factor, or 175 W with 20% Duty Factor. Do not use 200 W with 20% Duty Factor.
Peak Incident Power 200 W maximum*
Duty Factor 20% maximum
Rack Rack E220e 4 microTUBE-500 Screw Cap (500484)
Intensier 500141 required, installed on transducer (See Appendix A for details)
Water level (RUN scale) Level 6
Plate Denion 500484 E220e 4 microTUBE-500 Screw-Cap -9.9mm oset
*The E220evoluon can operate using a power combinaon of 200 W with 10% Duty Factor, or 175 W with 20% Duty Factor. Do not use 200 W with 20% Duty Factor.
CAUTION: All Covaris microTUBEs must operate within energy constraints. The power maximum levels are guides and should not be
exceeded. Each instrument has been tested with the microTUBE-500 for 2x the energy of the 150 bp DNA shearing protocol, and with
the truXTRAC® FFPE emulsicaon protocol. Operang outside of these limits or limits published in Covaris protocols may compromise
the integrity of the microTUBE-500.

3Part Number: 010526 Rev A
Covaris
June 2020
E220
LE-Series
Instrucons for Use
1. The microTUBE-500 has a closed septum. Load the microTUBE-500 into the microTUBE-500 Screw-Cap prep staon.
2. Unscrew the cap.
3. Add sample, being careful to keep the AFA Fiber in the microTUBE-500.
4. Screw the cap back on unl the stop point.
5. Load the microTUBE-500 into the appropriate rack or holder and process the sample.
6. Once nished, remove all sample from the microTUBE-500. Do not store sample in the microTUBE.
Revision History
Peak Incident Power 200 W maximum
Duty Factor 20% maximum
Rack Rack, 24 microTUBE-500 Screw Cap (500452)
Intensier 500141 required, installed on transducer (See Appendix A for details)
Water level (RUN scale) Level 6
E220 Plate Denion E220_500452 Rack 24 Place microTUBE-500 Screw-Cap +6mm oset
*The E220 can operate using a power combinaon of 200 W with 10% Duty Factor, or 175 W with 20% Duty Factor. Do not use 200 W with 20% Duty Factor.
Peak Incident Power 450 W maximum
Duty Cycle 30% maximum
Rack Rack, 24 microTUBE-500 Screw Cap (500452)
LE220 Water level (RUN scale) Level 6
LE220 Plate Denion LE220_500452 Rack 24 Place microTUBE-500 Screw-Cap +6mm oset
LE220-plus/R-plus Plate Denion LE220plus_500452 Rack 24 Place microTUBE-500 Screw-Cap +6mm oset
LE220Rsc-plus LE220PRSC_500452 Rack 24 Place microTUBE-500 Screw-Cap +6mm oset
NOTE: If the plate denion is not present on the system, contact Covaris Technical Support ([e-mail]) with the system
serial number.
Document Part # Revision Date Descripon of change
010526 A6/2020 New document

4Part Number: 010526 Rev A
Covaris
June 2020
Appendix A: Removing or Installing the Intensier (Covaris PN 500141) from a Covaris E System
The 500141 Intensier is a small inverted stainless steel cone centered over the E-Series transducer by four stainless wires. The wires
are held in place by a black plasc ring pressed into the transducer well.
If an AFA protocol requires “no Intensier”, please remove the Intensier, using the following steps:
1. Empty the water bath. Start the instrument and start the SonoLab™ soware.
2. Wait for the homing sequence to complete (the transducer will be lowered with the rack holder at the home posion, allowing
easy access to the Intensier).
3. Grasp opposite sides of plasc ring and gently pull the enre assembly out of the transducer well. Do not pull on the steel cone
or the wires. The ring is a fricon t in the well – no hardware is used to hold it in place.
The 500141 Intensier (le) shown installed in the E-Series transducer well and (right) removed. Note the “UP” marking at the center
of the Intensier.
If a protocol requires the Intensier to be present, simply reverse this process:
4. Align the black plasc ring with the perimeter of the transducer well. Note that the at side of the center cone (marked UP)
should be facing up (away from the transducer).
5. Gently press each secon of the ring into the well unl the ring is seated uniformly in contact with the transducer, with
approximately 2 mm of the ring evenly exposed above the transducer assembly. Do not press on the cone or wires. The
rotaon of the ring relave to the transducer assembly is not important.
6. Rell the tank. Degas and chill the water before proceeding.
Technical Support – Ongoing assistance with the operaon or applicaon of the equipment and/or troubleshoong is provided via:
• Telephone
- United States: Tel: [phone] during the hours of 9:00 a.m. to 5:00 p.m., Monday through Friday,
Eastern Standard Time (EST), Greenwich Mean Time (GMT-05:00)
- Europe: Tel: [phone], during the hours of 9:00 a.m. to 5:00 p.m., Monday through Friday, Greenwich Mean Time
• E-mail queries to [email protected] or applica[e-mail]