cytiva miniVE User manual

miniVE
Operating Instructions
Original instructions
cytiva.com

Table of Contents
1Introduction ........................................................................................................ 3
1.1 About this manual ......................................................................................................................................... 4
1.2 Important user information ....................................................................................................................... 5
2 Safety instructions ............................................................................................. 7
2.1 Safety precautions ........................................................................................................................................ 8
2.2 Labels ................................................................................................................................................................. 13
2.3 Emergency procedures ............................................................................................................................... 14
3 System description ............................................................................................ 15
3.1 Instrument overview .................................................................................................................................... 16
3.2 Detailed description ..................................................................................................................................... 17
4 Installation .......................................................................................................... 19
5 Operation ............................................................................................................ 20
5.1 Electrophoresis module .............................................................................................................................. 22
5.1.1 Prepare the electrophoresis module ................................................................................................... 23
5.1.2 Pre-cast gels ................................................................................................................................................... 30
5.1.3 Final electrophoresis assembly .............................................................................................................. 32
5.1.4 Electrophoresis run ..................................................................................................................................... 34
5.1.5 After electrophoresis .................................................................................................................................. 35
5.2 Electrotransfer module ............................................................................................................................... 36
5.2.1 Prepare the electrotransfer module .................................................................................................... 37
5.2.2 Final electrotransfer assembly ............................................................................................................... 41
5.2.3 Electrotransfer run ....................................................................................................................................... 43
5.2.4 After electrotransfer ................................................................................................................................... 44
6 Maintenance ....................................................................................................... 45
7 Troubleshooting ................................................................................................. 47
7.1 Electrophoresis troubleshooting ............................................................................................................ 48
7.2 Electrotransfer troubleshooting .............................................................................................................. 51
8 Reference information ....................................................................................... 54
8.1 Specification .................................................................................................................................................... 55
8.2 Ordering information ................................................................................................................................... 57
8.3 Recycling information .................................................................................................................................. 58
8.4 Regulatory information ............................................................................................................................... 59
8.4.1 Contact information .................................................................................................................................... 60
8.4.2 European Union and European Economic Area .............................................................................. 61
8.4.3 Eurasian Economic Union
Евразийский экономический союз .................................................................................................. 62
8.4.4 Declaration of Hazardous Substances (DoHS) ................................................................................ 64
8.5 Health and Safety Declaration Form ...................................................................................................... 66
Index ........................................................................................................................... 68
Table of Contents
2 miniVE Operating Instructions 29281778 AB

1 Introduction
About this chapter
This chapter contains important user information, descriptions of safety notices, regu-
latory information, and intended use of the miniVE system.
In this chapter
Section See page
1.1 About this manual 4
1.2 Important user information 5
1 Introduction
miniVE Operating Instructions 29281778 AB 3

1.1 About this manual
Purpose of this manual
The Operating Instructions provide you with the information needed to install, operate
and maintain the product in a safe way.
Scope of this manual
The Operating Instructions cover the miniVE instrument. The illustration below shows
the miniVE instrument.
Illustrations
The images and annotations in this document are for illustrative purposes only. The
configuration of individual products may vary, and therefore illustrations may not
reflect the actual system delivered.
1 Introduction
1.1 About this manual
4 miniVE Operating Instructions 29281778 AB

1.2 Important user information
Read this before operating the
product
All users must read the entire Operating Instructions before installing, oper-
ating or maintaining the product.
Always keep the Operating Instructions at hand when operating the product.
Do not operate the product in any other way than described in the user documenta-
tion. If you do, you may be exposed to hazards that can lead to personal injury and you
may cause damage to the equipment.
Intended use of the product
miniVE is intended for protein and nucleic acid electrophoresis under commonly used
denaturing and non-denaturing conditions. miniVE can be combined with an optional
blotting module. miniVE Blot Module is primarily intended for blotting of proteins and
nucleic acids at laboratory-scale. The system can be used for a variety of research
purposes to fulfill the needs of the users in academia and in life sciences industry.
miniVE shall not be used in any clinical procedures, or for diagnostic purposes.
Prerequisites
In order to operate the miniVE in the way it is intended:
•The user must have a general understanding of electrophoresis and blotting techni-
ques.
•The user must read and understand the Safety Instructions chapter in the Oper-
ating Instructions.
•miniVE must be installed in accordance with the instructions in the Operating
Instructions.
Safety notices
This user documentation contains safety notices (WARNING, CAUTION, and NOTICE)
concerning the safe use of the product. See definitions below.
1 Introduction
1.2 Important user information
miniVE Operating Instructions 29281778 AB 5

WARNING
WARNING indicates a hazardous situation which, if not avoided,
could result in death or serious injury. It is important not to proceed
until all stated conditions are met and clearly understood.
CAUTION
CAUTION indicates a hazardous situation which, if not avoided,
could result in minor or moderate injury. It is important not to
proceed until all stated conditions are met and clearly understood.
NOTICE
NOTICE indicates instructions that must be followed to avoid
damage to the product or other equipment.
Notes and tips
Note: A note is used to indicate information that is important for trouble-free and
optimal use of the product.
Tip: A tip contains useful information that can improve or optimize your proce-
dures.
1 Introduction
1.2 Important user information
6 miniVE Operating Instructions 29281778 AB

2 Safety instructions
About this chapter
This chapter describes safety precautions, labels and symbols that are attached to the
equipment. In addition, the chapter describes emergency and recovery procedures,
and provides recycling information.
Important
WARNING
Before installing, operating or maintaining the product, all
users must read and understand the entire contents of this
chapter to become aware of the hazards involved.
In this chapter
Section See page
2.1 Safety precautions 8
2.2 Labels 13
2.3 Emergency procedures 14
2 Safety instructions
miniVE Operating Instructions 29281778 AB 7

2.1 Safety precautions
Introduction
miniVE is powered by an external power supply and handles materials that are consid-
ered hazardous material. Before installing, operating or maintaining the system, you
must be aware of the hazards described in this manual.
Follow the instructions provided to avoid injury to the operator or other
personnel, to the product, or to other equipment in the area.
The safety precautions in this section are grouped into the following categories:
•General precautions
•Personal protection
•Using flammable liquids
•Installing and moving the product
•Power supply
•System operation
•Maintenance
Always follow the instructions below to avoid injury when using the miniVE.
General precautions
WARNING
Before installing, operating or maintaining the product, all
users must read and understand the entire contents of this
chapter to become aware of the hazards involved.
WARNING
Only properly trained personnel may operate and maintain the
product.
WARNING
Do not operate the miniVE in any other way than described in the
miniVE Operating Instructions.
2 Safety instructions
2.1 Safety precautions
8 miniVE Operating Instructions 29281778 AB

WARNING
Do not damage the power supply cord by bending, twisting, heating
or allowing them to become pinned under the equipment. Using
damaged power cords could result in fire or electric shock.
If the power supply cords are damaged, contact your local Cytiva
representative for replacements.
WARNING
Use only approved parts. Only spare parts and accessories that
are approved or supplied by Cytiva may be used for maintaining or
servicing the product.
WARNING
The safety lid must be in place before connecting the power leads
to a power supply.
WARNING
The high voltage power supply must always be disconnected when
the safety lid of the electrophoresis unit is taken off. The high
voltage power supply must never be switched on unless the safety
lid is on the electrophoresis unit.
WARNING
Never exceed the operating limits stated in this document and on
the system label. Operation of the product outside these limits can
damage equipment and cause personal injury or death.
WARNING
Any liquid on the equipment must be dried off before connecting
the power supply.
CAUTION
Handle the glass components with care! Wear appropriate
personal protective equipment (PPE).
2 Safety instructions
2.1 Safety precautions
miniVE Operating Instructions 29281778 AB 9

CAUTION
Never introduce anti-freeze or any organic solvent into any part of
the instrument. Organic solvents will cause irreparable damage to
the instrument.
CAUTION
When lifting and moving the instrument be careful not to drop it.
This may cause injury.
Personal protection
WARNING
Always use appropriate Personal Protective Equipment (PPE)
during operation and maintenance of this product.
WARNING
Hazardous substances and biological agents. When using
hazardous chemical and biological agents, take all suitable protec-
tive measures, such as wearing protective clothing, glasses and
gloves resistant to the substances used. Follow local and/or
national regulations for safe operation and maintenance of this
product.
WARNING
Spread of biological agents. The operator must take all neces-
sary actions to avoid spreading hazardous biological agents. The
facility must comply with the national code of practice for
biosafety.
Using flammable liquids
WARNING
A fume hood or similar ventilation system shall be installed when
flammable or noxious substances are used.
2 Safety instructions
2.1 Safety precautions
10 miniVE Operating Instructions 29281778 AB

Installing and moving the product
CAUTION
Turn off the power switch and remove connecting cables before
moving the equipment.
CAUTION
When lifting and moving the instrument be careful not to drop it.
This may cause injury.
CAUTION
Make sure that the system is placed on a stable, level bench with
adequate space for ventilation.
CAUTION
The electrophoresis unit is heavy, especially when filled with buffer.
Handle the unit with care to avoid personal injury.
Power supply
WARNING
Power cord. Only use power cords with approved plugs delivered
or approved by Cytiva.
WARNING
Make sure that there is access to the instrument power supply cord
at all times.
WARNING
Disconnect power. Always disconnect power from the instru-
ment before replacing any component on the instrument, unless
stated otherwise in the user documentation.
2 Safety instructions
2.1 Safety precautions
miniVE Operating Instructions 29281778 AB 11

Operation
CAUTION
Do not operate with buffer temperature above 75°C.
WARNING
Acrylamide is a neurotoxin. Always wear gloves and observe all
laboratory safety procedures.
Maintenance
WARNING
Decontaminate before maintenance. To avoid personnel being
exposed to potentially hazardous substances, make sure that the
miniVE is properly decontaminated and sanitized before mainte-
nance or service.
WARNING
Disconnect power. Always disconnect power from the instru-
ment before performing any maintenance task.
WARNING
Decommissioning. Decontaminate the equipment before
decommissioning to make sure that hazardous residues are
removed.
2 Safety instructions
2.1 Safety precautions
12 miniVE Operating Instructions 29281778 AB

2.2 Labels
Introduction
This section describes the system label and other safety or regulatory labels that are
attached to the product.
Description of symbols on the system
label
The table below describes the various symbols that may be found on the system label.
Label Meaning
Warning! Read the user documentation before using the system.
Do not open any covers or replace parts unless specifically stated
in the user documentation.
Serial no.: Serial number of the product
Manufac-
tured:
Year (YYYY) and month (MM) of manufacture
Safety labels
The table below describes the various symbols that may be found on the product.
Symbol/text Description
Warning! Read the user documentation before using
the system. Do not open any covers or replace parts
unless specifically stated in the user documentation.
Warning! High Voltage. Always make sure that the
system is disconnected from electric power before
removing the lid.
2 Safety instructions
2.2 Labels
miniVE Operating Instructions 29281778 AB 13

2.3 Emergency procedures
Introduction
This section describes how to shut down the product in an emergency situation, and
the procedure for restarting the product.
The section also describes the result in the event of power failure.
Precautions
WARNING
Make sure that there is access to the instrument power supply cord
at all times.
Emergency shutdown
In an emergency situation, shut down the power supply in accordance with its emer-
gency procedure.
Power failure
In case of power failure to the product, the run is interrupted immediately.
Restart after emergency shutdown or
power failure
To restart the run after an emergency shutdown or power failure, follow these steps:
Step Action
1Make sure all connections are in place.
2Start the power supply as described in the power supply's User Manual.
2 Safety instructions
2.3 Emergency procedures
14 miniVE Operating Instructions 29281778 AB

3 System description
About this chapter
This chapter gives an overview of the miniVE instrument, and a brief description of its
function.
In this chapter
Section See page
3.1 Instrument overview 16
3.2 Detailed description 17
3 System description
miniVE Operating Instructions 29281778 AB 15

3.1 Instrument overview
Introduction to miniVE
The miniVE is a vertical electrophoresis electrotransfer instrument.
This section gives an overview of the miniVE, and the optional miniVE Blot Module.
Illustration of the instrument
The illustration below shows the miniVE instrument.
miniVE is powered using a separate power supply.
3 System description
3.1 Instrument overview
16 miniVE Operating Instructions 29281778 AB

3.2 Detailed description
Introduction
This section gives an overview of miniVE, and miniVE Blot Module.
Illustration of miniVE
The illustration below shows the locations and gives brief descriptions of the miniVE
instrument.
1
2
3
4
5
Part Function
1 Color-coded leads
2 Safety lid
3 Electrophoresis module
4 Banana plug connectors
5 Tank
miniVE requires a power supply with a minimum rating of 50 mA and 250 V.
3 System description
3.2 Detailed description
miniVE Operating Instructions 29281778 AB 17

Illustration of miniVE Blot Module
The illustration below shows the locations of the optional miniVE Blot Module.
1
2
Part Function
1 Black cathode side of blotting module
2 Red anode side of blotting module
3 System description
3.2 Detailed description
18 miniVE Operating Instructions 29281778 AB

4 Installation
Introduction
This chapter provides required information to enable users and service personnel to
unpack miniVE.
Safety precautions
CAUTION
When lifting and moving the instrument be careful not to drop it.
This may cause injury.
CAUTION
Make sure that the system is placed on a stable, level bench with
adequate space for ventilation.
Unpacking procedure
Unwrap all packages carefully.
Inspect all visible parts for damage or missing pieces. If any damage is observed, record
this on the receiving documents and inform your Cytiva representative. Make sure to
keep all packing material for damage claims or to use should it become necessary to
return the unit.
4 Installation
miniVE Operating Instructions 29281778 AB 19

5 Operation
About this chapter
This chapter gives instructions on how to safely operate the product.
Safety precautions
WARNING
Never exceed the operating limits stated in this document and on
the system label. Operation of the product outside these limits can
damage equipment and cause personal injury or death.
WARNING
The safety lid must be in place before connecting the power leads
to a power supply.
WARNING
The high voltage power supply must always be disconnected when
the safety lid of the electrophoresis unit is taken off. The high
voltage power supply must never be switched on unless the safety
lid is on the electrophoresis unit.
WARNING
Any liquid on the equipment must be dried off before connecting
the power supply.
CAUTION
Never introduce anti-freeze or any organic solvent into any part of
the instrument. Organic solvents will cause irreparable damage to
the instrument.
5 Operation
20 miniVE Operating Instructions 29281778 AB