cytiva SE 250 Mighty Small II User manual

SE 250/260 Mighty Small II
Operating Instructions
Original instructions
cytiva.com

Table of Contents
1Introduction ........................................................................................................ 3
1.1 About this manual ......................................................................................................................................... 4
1.2 Important user information ....................................................................................................................... 5
2 Safety information ............................................................................................. 7
2.1 Safety precautions ........................................................................................................................................ 8
2.2 Labels ................................................................................................................................................................. 15
2.3 Emergency procedures ............................................................................................................................... 16
3 System description ............................................................................................ 17
4 Installation .......................................................................................................... 19
5 Operation ............................................................................................................ 20
5.1 Preparations .................................................................................................................................................... 22
5.2 Assembly ........................................................................................................................................................... 25
5.3 Electrophoresis run ....................................................................................................................................... 31
6 Maintenance ....................................................................................................... 33
7 Troubleshooting ................................................................................................. 35
8 Reference information ....................................................................................... 38
8.1 Specifications .................................................................................................................................................. 39
8.2 Recycling information .................................................................................................................................. 40
8.3 Regulatory information ............................................................................................................................... 41
8.3.1 Contact information .................................................................................................................................... 42
8.3.2 European Union and European Economic Area .............................................................................. 43
8.3.3 Eurasian Economic Union
Евразийский экономический союз .................................................................................................. 44
8.3.4 Declaration of Hazardous Substances (DoHS) ................................................................................ 46
8.4 Ordering information ................................................................................................................................... 48
8.5 Health and Safety Declaration Form ...................................................................................................... 49
Index ........................................................................................................................... 51
Table of Contents
2 SE 250/260 Mighty Small II Operating Instructions 29281629 AB

1 Introduction
About this chapter
This chapter contains important user information, descriptions of safety notices, regu-
latory information, and intended use of the SE 250/260 Mighty Small II.
In this chapter
Section See page
1.1 About this manual 4
1.2 Important user information 5
1 Introduction
SE 250/260 Mighty Small II Operating Instructions 29281629 AB 3

1.1 About this manual
Purpose of this manual
The Operating instructions provide you with the information needed to install, operate
and maintain the product in a safe way.
Scope of this manual
The Operating Instructions covers the SE 250/260 Mighty Small II. The illustration
below shows the SE 250/260 Mighty Small II.
Illustrations
The images and annotations in this document are for illustrative purposes only. The
configuration of individual products may vary, and therefore illustrations may not
reflect the actual system delivered.
1 Introduction
1.1 About this manual
4 SE 250/260 Mighty Small II Operating Instructions 29281629 AB

1.2 Important user information
Read this before operating the
product
All users must read the entire Operating instruction before installing, oper-
ating or maintaining the product.
Always keep the Operating instruction at hand when operating the product.
Do not operate the product in any other way than described in the user documenta-
tion. If you do, you may be exposed to hazards that can lead to personal injury and you
may cause damage to the equipment.
Intended use of the product
The SE 250/260 Mighty Small II small format vertical slab gel unit is intended for rapid
electrophoresis of protein or nucleic acid samples. Most samples can be run in 45
minutes, and only a minimal amount of sample is required.
The SE 250/260 Mighty Small II accommodates one or two 10 × 8 cm gel sandwiches.
The SE 250/260 Mighty Small II accommodates one or two 10 × 8 cm or 10 × 10.5 cm gel
sandwiches. In both SE 250/260 Mighty Small II units, the upper buffer chamber is
formed when the notched side of a gel sandwich is sealed against the silicone rubber
gasket.
The upper buffer chamber core serves as a heat exchanger if cooling is required. The
core is hollow and equipped with ports on either side for coolant circulation.
SE 250/260 Mighty Small II is intended for research use only, and shall not be used in
any clinical procedures, or for diagnostic purposes.
Prerequisites
In order to operate the SE 250/260 Mighty Small II in the way it is intended:
•The user must have a general understanding of gel electrophoresis.
•The user must read and understand the Safety Instructions chapter in the Oper-
ating Instructions.
•The SE 250/260 Mighty Small II must be installed in accordance with the instruc-
tions in the Operating Instructions.
1 Introduction
1.2 Important user information
SE 250/260 Mighty Small II Operating Instructions 29281629 AB 5

Notes and tips
Note: A note is used to indicate information that is important for trouble-free and
optimal use of the product.
Tip: A tip contains useful information that can improve or optimize your proce-
dures.
1 Introduction
1.2 Important user information
6 SE 250/260 Mighty Small II Operating Instructions 29281629 AB

2 Safety information
About this chapter
This chapter describes safety precautions, labels and symbols that are attached to the
equipment. In addition, the chapter describes emergency and recovery procedures,
and provides recycling information.
Important
WARNING
Before installing, operating or maintaining the product, all
users must read and understand the entire contents of this
chapter to become aware of the hazards involved.
In this chapter
Section See page
2.1 Safety precautions 8
2.2 Labels 15
2.3 Emergency procedures 16
2 Safety information
SE 250/260 Mighty Small II Operating Instructions 29281629 AB 7

2.1 Safety precautions
Introduction
The SE 250/260 Mighty Small II is powered by an external power supply. Before instal-
ling, operating or maintaining the system, you must be aware of the hazards described
in this manual.
Follow the instructions provided to avoid injury to the operator or other
personnel, to the product, or to other equipment in the area.
The safety precautions in this section are grouped into the following categories:
•General precautions
•Personal protection
•Using flammable liquids
•Installing and moving the product
•Power supply
•System operation
•Maintenance
Always follow the instructions below to avoid injury when using the SE 250/260 Mighty
Small II.
Definitions
This user documentation contains safety notices (WARNING, CAUTION, and NOTICE)
concerning the safe use of the product. See definitions below.
WARNING
WARNING indicates a hazardous situation which, if not avoided,
could result in death or serious injury. It is important not to proceed
until all stated conditions are met and clearly understood.
CAUTION
CAUTION indicates a hazardous situation which, if not avoided,
could result in minor or moderate injury. It is important not to
proceed until all stated conditions are met and clearly understood.
NOTICE
NOTICE indicates instructions that must be followed to avoid
damage to the product or other equipment.
2 Safety information
2.1 Safety precautions
8 SE 250/260 Mighty Small II Operating Instructions 29281629 AB

General precautions
WARNING
Before installing, operating or maintaining the product, all
users must read and understand the entire contents of this
chapter to become aware of the hazards involved.
WARNING
Only properly trained personnel may operate and maintain the
product.
WARNING
Do not operate the SE 250/260 Mighty Small II in any other way
than described in the SE 250/260 Mighty Small II Operating
Instructions.
WARNING
Do not damage the power supply cord by bending, twisting, heating
or allowing them to become pinned under the equipment. Using
damaged power cords could result in fire or electric shock.
If the power supply cords are damaged, contact your local Cytiva
representative for replacements.
WARNING
The safety lid must be in place before connecting the power leads
to a power supply.
WARNING
Any liquid on the equipment must be dried off before connecting
the power supply.
2 Safety information
2.1 Safety precautions
SE 250/260 Mighty Small II Operating Instructions 29281629 AB 9

CAUTION
Circulate only water or 50/50 water/ethylene glycol through the
heat exchanger. Never use anti-freeze or any organic solvent in the
heat exchanger.
CAUTION
Never introduce anti-freeze or any organic solvent into any part of
the instrument. Organic solvents will cause irreparable damage to
the instrument.
Personal protection
WARNING
Always use appropriate Personal Protective Equipment (PPE)
during operation and maintenance of this product.
WARNING
Hazardous substances and biological agents. When using
hazardous chemical and biological agents, take all suitable protec-
tive measures, such as wearing protective clothing, glasses and
gloves resistant to the substances used. Follow local and/or
national regulations for safe operation and maintenance of this
product.
WARNING
Spread of biological agents. The operator must take all neces-
sary actions to avoid spreading hazardous biological agents. The
facility must comply with the national code of practice for
biosafety.
CAUTION
Handle the glass components with care! Wear appropriate
personal protective equipment (PPE).
2 Safety information
2.1 Safety precautions
10 SE 250/260 Mighty Small II Operating Instructions 29281629 AB

Using flammable liquids
WARNING
A fume hood or similar ventilation system shall be installed when
flammable or noxious substances are used.
Installing and moving the product
CAUTION
When lifting and moving the instrument be careful not to drop it.
This may cause injury.
CAUTION
Make sure that the system is placed on a stable, level bench with
adequate space for ventilation.
CAUTION
Turn off the power switch and remove connecting cables before
moving the equipment.
CAUTION
The electrophoresis unit is heavy, especially when filled with buffer.
Handle the unit with care to avoid personal injury.
Power supply
WARNING
Power cord. Only use power cords with approved plugs delivered
or approved by Cytiva.
WARNING
The safety lid must be in place before connecting the power leads
to a power supply.
2 Safety information
2.1 Safety precautions
SE 250/260 Mighty Small II Operating Instructions 29281629 AB 11

WARNING
Make sure that there is access to the instrument power supply cord
at all times.
Operation
WARNING
The high voltage power supply must always be disconnected when
the safety lid of the electrophoresis unit is taken off. The high
voltage power supply must never be switched on unless the safety
lid is on the electrophoresis unit.
WARNING
Acrylamide is a neurotoxin. Always wear gloves and observe all
laboratory safety procedures.
WARNING
Never exceed the operating limits stated in this document and on
the system label. Operation of the product outside these limits can
damage equipment and cause personal injury or death.
WARNING
Turn all power supply controls off and disconnect the power leads
before removing the safety lid.
WARNING
Always disconnect the high voltage leads from the power supply
before removing the lid from the unit.
CAUTION
Circulate coolant through the heat exchanger to minimize heating.
Overheating will cause irreparable damage to the unit! Do not
connect the heat exchanger to a water tap or any coolant source
where the water pressure is unregulated.
2 Safety information
2.1 Safety precautions
12 SE 250/260 Mighty Small II Operating Instructions 29281629 AB

CAUTION
Do not operate with buffer temperatures above the maximum
specified technical specifications. Overheating will cause irrepar-
able damage to the unit!
CAUTION
Devices must only be operated with a certified power supply
capable of limiting the voltage and current to specified ratings.
NOTICE
After initial monitoring, do not leave the unit unattended for more
than 45 min before checking the progress of the bands and the
buffer level.
NOTICE
If running only one gel, block off the unused part of the core with a
glass plate. Do not fill this side with buffer.
Maintenance
WARNING
Decontaminate before maintenance. To avoid personnel being
exposed to potentially hazardous substances, make sure that the
SE 250/260 Mighty Small II is properly decontaminated and sani-
tized before maintenance or service.
WARNING
Only spare parts and accessories that are approved or supplied by
Cytiva may be used for maintaining or servicing the product.
WARNING
Disconnect power. Always disconnect power from the instru-
ment before performing any maintenance task.
2 Safety information
2.1 Safety precautions
SE 250/260 Mighty Small II Operating Instructions 29281629 AB 13

WARNING
Decommissioning. Decontaminate the equipment before
decommissioning to make sure that hazardous residues are
removed.
NOTICE
Cleaning. Keep the exterior of the instrument dry and clean. Wipe
regularly with a soft damp tissue and, if necessary, a mild cleaning
agent. Let the instrument dry completely before use.
2 Safety information
2.1 Safety precautions
14 SE 250/260 Mighty Small II Operating Instructions 29281629 AB

2.2 Labels
Introduction
This section describes the system label and other safety or regulatory labels that are
attached to the product.
Description of symbols on the system
label
The table below describes the various symbols that may be found on the system label.
Label Meaning
Warning! Read the user documentation before using the system.
Do not open any covers or replace parts unless specifically stated
in the user documentation.
Serial no.: Serial number of the product
Manufac-
tured:
Year (YYYY) and month (MM) of manufacture
Safety labels
The table below describes the various symbols that may be found on the product.
Symbol/text Description
Warning! Read the user documentation before using
the system. Do not open any covers or replace parts
unless specifically stated in the user documentation.
Warning! High Voltage. Always make sure that the
system is disconnected from electric power before
removing the lid.
2 Safety information
2.2 Labels
SE 250/260 Mighty Small II Operating Instructions 29281629 AB 15

2.3 Emergency procedures
Introduction
This section describes how to shut down of the SE 250/260 Mighty Small II in an emer-
gency situation, and the procedure for restarting the SE 250/260 Mighty Small II.
The section also describes the result in the event of power failure.
Precautions
WARNING
Make sure that there is access to the instrument power supply cord
at all times.
Emergency shutdown
In an emergency situation, shut down the power supply in accordance with its emer-
gency procedure.
Power failure
In case of power failure to the SE 250/260 Mighty Small II, the run is interrupted imme-
diately.
Restart after emergency shutdown or
power failure
To restart the run after an emergency shutdown or power failure, follow these steps:
Step Action
1Make sure all connections are in place.
2Start the power supply as described in the power supply's User Manual.
2 Safety information
2.3 Emergency procedures
16 SE 250/260 Mighty Small II Operating Instructions 29281629 AB

3 System description
About this chapter
This chapter gives an overview of the SE 250/260 Mighty Small II.
Illustration of the instrument
The illustration below shows the SE 250/260 Mighty Small II instrument.
1
2
3
4
5
6
8
7
Part Function
1 Color-coded leads (2)
2 Safety lid
3 System description
SE 250/260 Mighty Small II Operating Instructions 29281629 AB 17

Part Function
3 Upper buffer chamber core
4 Foam gasket
5 Coolant port (2)
6 Spring clamps (4)
7 Positioning tabs
8 SE 250/260 Mighty Small II Lower buffer chamber
The following parts are included, but not shown in the illustration:
•Glass plates
•Notched alumina plates
•Gel seal, 1/4 oz.
•Spacer-Mate
•Well-locating decal
Note: Power supply with a minimum rating of 250 V, 50 mA, constant current or
constant voltage is required, but not included.
3 System description
18 SE 250/260 Mighty Small II Operating Instructions 29281629 AB

4 Installation
About this chapter
This chapter provides required information to enable users and service personnel to
unpack the SE 250/260 Mighty Small II instrument.
Safety precautions
CAUTION
When lifting and moving the instrument be careful not to drop it.
This may cause injury.
CAUTION
Make sure that the system is placed on a stable, level bench with
adequate space for ventilation.
Unpacking procedure
Unwrap all packages carefully.
Inspect all visible parts for damage or missing pieces. If any damage is observed, record
this on the receiving documents and inform your Cytiva representative. Make sure to
keep all packing material for damage claims or to use should it become necessary to
return the unit.
4 Installation
SE 250/260 Mighty Small II Operating Instructions 29281629 AB 19

5 Operation
About this chapter
This chapter gives instructions on how to operate the product in a safe way.
Safety precautions
WARNING
The high voltage power supply must always be disconnected when
the safety lid of the electrophoresis unit is taken off. The high
voltage power supply must never be switched on unless the safety
lid is on the electrophoresis unit.
WARNING
Acrylamide is a neurotoxin. Always wear gloves and observe all
laboratory safety procedures.
WARNING
Never exceed the operating limits stated in this document and on
the system label. Operation of the product outside these limits can
damage equipment and cause personal injury or death.
CAUTION
Do not operate with buffer temperatures above the maximum
specified technical specifications. Overheating will cause irrepar-
able damage to the unit!
CAUTION
Circulate only water or 50/50 water/ethylene glycol through the
heat exchanger. Never use anti-freeze or any organic solvent in the
heat exchanger.
5 Operation
20 SE 250/260 Mighty Small II Operating Instructions 29281629 AB