cytiva F9-R User manual

Fraction collector F9-R
Operating Instructions
Original instructions
F9-R
cytiva.com

Table of Contents
1 Introduction ........................................................................................................ 3
1.1 Important user information ....................................................................................................................... 4
1.2 About this manual ......................................................................................................................................... 5
1.3 Associated documentation ....................................................................................................................... 6
2 Safety instructions ............................................................................................. 8
2.1 Safety precautions ........................................................................................................................................ 9
2.2 Labels and symbols ....................................................................................................................................... 14
2.3 Emergency procedures ............................................................................................................................... 16
3 Overview .............................................................................................................. 18
3.1 Function ............................................................................................................................................................ 19
3.2 Illustrations ...................................................................................................................................................... 20
3.3 Tubes .................................................................................................................................................................. 23
4 Installation .......................................................................................................... 25
4.1 Site preparation .............................................................................................................................................. 26
4.1.1 Delivery and storage ................................................................................................................................... 27
4.1.2 Space requirements .................................................................................................................................... 28
4.1.3 Site requirements ......................................................................................................................................... 30
4.2 Connections to the ÄKTA instrument .................................................................................................... 31
4.2.1 Power and communication ...................................................................................................................... 32
4.2.2 Tubing connections ..................................................................................................................................... 36
4.3 Delay volume ................................................................................................................................................... 38
4.4 Performance test ........................................................................................................................................... 39
5 Operation ............................................................................................................ 40
6 Maintenance ....................................................................................................... 45
7 Reference information ....................................................................................... 46
7.1 Specifications .................................................................................................................................................. 47
7.2 Chemical resistance ..................................................................................................................................... 48
7.3 Recycling information .................................................................................................................................. 49
7.4 Regulatory information ............................................................................................................................... 50
7.4.1 Contact information .................................................................................................................................... 51
7.4.2 European Union and European Economic Area .............................................................................. 52
7.4.3 Eurasian Economic Union
Евразийский экономический союз .................................................................................................. 53
7.4.4 Regulations for North America ............................................................................................................... 55
7.4.5 Regulatory statements .............................................................................................................................. 56
7.4.6 Declaration of Hazardous Substances (DoHS) ................................................................................ 57
7.5 Health and Safety Declaration Form ...................................................................................................... 59
Index ........................................................................................................................... 61
Table of Contents
2 Fraction collector F9-R Operating Instructions 29656880 AA

1 Introduction
About this chapter
This chapter contains information about this manual and associated user documenta-
tion, important user information and intended use of the product.
In this chapter
Section See page
1.1 Important user information 4
1.2 About this manual 5
1.3 Associated documentation 6
1 Introduction
Fraction collector F9-R Operating Instructions 29656880 AA 3

1.1 Important user information
Read this before operating the
product
All users must read the entire Operating Instructions before installing, oper-
ating or maintaining the product.
Always keep the Operating Instructions at hand when operating the product.
Do not install, operate, or perform maintenance on the product in any other way than
described in the user documentation. If you do, you may be exposed or expose others
to hazards that can lead to personal injury and you may cause damage to the equip-
ment.
Intended use
Fraction collector F9-R is an automated fraction collector intended for the collection of
fractions from purification runs. It is intended for research use only, and shall not be
used in clinical procedures, or for diagnostic purposes.
Prerequisites
In order to operate Fraction collector F9-R in the way it is intended:
•The user must know how to use a computer with Microsoft® Windows®.
•The user should understand the concepts of liquid chromatography.
•The user must be familiar with the purification system and have read the Operating
Instructions for the system.
•The user must read and understand the Safety Instructions chapter in the Oper-
ating Instructions.
•Fraction collector F9-R must be installed in accordance with the site requirements
and instructions in the Operating Instructions.
1 Introduction
1.1 Important user information
4 Fraction collector F9-R Operating Instructions 29656880 AA

1.2 About this manual
Purpose of this manual
The Operating Instructions manual provides information needed to install, operate and
maintain the product in a safe way.
Scope of this manual
The Operating Instructions manual covers Fraction collector F9-R, in the manual also
referred to as the product.
Typographical conventions
Software items are identified in the text by bold italic text.
Hardware items are identified in the text by bold text.
In electronic format, references in italics are clickable hyperlinks.
Notes and tips
Note: A note is used to indicate information that is important for trouble-free and
optimal use of the product.
Tip: A tip contains useful information that can improve or optimize your proce-
dures.
1 Introduction
1.2 About this manual
Fraction collector F9-R Operating Instructions 29656880 AA 5

1.3 Associated documentation
Introduction
This section describes the user documentation delivered with the product, and how to
find related literature that can be downloaded or ordered from Cytiva.
User documentation for Fraction
collector F9-R
The user documentation is listed in the table below. The manuals are delivered on
removable media together with the product, but can also be downloaded from
cytiva.com.
Documentation Main contents
Fraction collector F9-R Operating
Instructions
Instructions needed to install and
operate the Fraction collector F9-R in a
correct and safe way.
Translations of Fraction collector F9-R
Operating Instructions
Translated versions of the original
instructions.
User documentation for compatible
purification systems
This section lists examples of user documentation for purification systems that can be
used together with this product. The manuals can be downloaded from cytiva.com.
Documentation Main contents
Operating Instructions for the ÄKTA™
instrument
Instructions needed to install and
operate the ÄKTA instrument in a
correct and safe way.
Translated versions are delivered
together with the printed Operating
Instructions and are also found on
cytiva.com.
Cue Cards (if available) for the ÄKTA
instrument
Condensed information on how to
operate the ÄKTA instrument.
User Manual for the ÄKTA instrument Additional information in order to get
the optimal performance from the
system.
1 Introduction
1.3 Associated documentation
6 Fraction collector F9-R Operating Instructions 29656880 AA

Help and user documentation within
the UNICORN software
For help regarding UNICORN™, mark the area of interest in the software and press F1.
It is then possible to navigate further to find, for example, software manuals.
Tip: There are specific help texts for instructions that only can be reached by
marking the instruction and pressing F1.
1 Introduction
1.3 Associated documentation
Fraction collector F9-R Operating Instructions 29656880 AA 7

2 Safety instructions
About this chapter
This chapter describes safety precautions, labels and symbols that are attached to the
equipment. In addition, the chapter describes emergency and recovery procedures.
In this chapter
Section See page
2.1 Safety precautions 9
2.2 Labels and symbols 14
2.3 Emergency procedures 16
Important
WARNING
Before installing, operating or maintaining the product, all
users must read and understand the entire contents of this
chapter to become aware of the hazards involved.
2 Safety instructions
8 Fraction collector F9-R Operating Instructions 29656880 AA

2.1 Safety precautions
Introduction
Fraction collector F9-R handles materials that can be hazardous.
Before installing, operating or maintaining the system, you must be aware of the
hazards described in this manual.
Definitions
This user documentation contains safety notices (WARNING, CAUTION, and NOTICE)
concerning the safe use of the product. See definitions below.
WARNING
WARNING indicates a hazardous situation which, if not avoided,
could result in death or serious injury. It is important not to proceed
until all stated conditions are met and clearly understood.
CAUTION
CAUTION indicates a hazardous situation which, if not avoided,
could result in minor or moderate injury. It is important not to
proceed until all stated conditions are met and clearly understood.
NOTICE
NOTICE indicates instructions that must be followed to avoid
damage to the product or other equipment.
General precautions
WARNING
Do not operate Fraction collector F9-R in any other way than
described in the Operating Instructions.
WARNING
Operation and user maintenance of Fraction collector F9-R should
be performed by properly trained personnel only.
2 Safety instructions
2.1 Safety precautions
Fraction collector F9-R Operating Instructions 29656880 AA 9

WARNING
Accessories. Use only accessories supplied or recommended by
Cytiva.
WARNING
Do not use Fraction collector F9-R if it is not working properly, or if
it has suffered any damage, for example:
•damage to the power cord or its plug
•damage caused by dropping the equipment
•damage caused by splashing liquid onto it
Flammable liquids
CAUTION
Explosion hazard during fractionation of flammable liquids.
Do not use a covered fraction collector (Fraction collector F9-C) to
fractionate flammable liquids. When using an open fraction
collector (Fraction collector F9-R), do not place the fraction
collector in a closed compartment and make sure that the room
ventilation meets the local requirements.
CAUTION
Fractionation with flammable liquids using Fraction
collector F9-R. Before you start the run, make sure that the tubes
in the fraction collector are of correct size according to fraction
size settings.
CAUTION
Moving tubes with flammable liquids. Move tubes filled with
flammable liquids carefully, to prevent spillages that may come in
contact with an ignition source.
2 Safety instructions
2.1 Safety precautions
10 Fraction collector F9-R Operating Instructions 29656880 AA

Personal protection
CAUTION
Always use appropriate personal protective equipment during
operation and maintenance of Fraction collector F9-R.
CAUTION
Hazardous substances. When using hazardous chemical and
biological agents, take all suitable protective measures, such as
wearing protective glasses and gloves resistant to the substances
used. Follow local and/or national regulations for safe operation,
maintenance and decommissioning of the equipment.
Installing the fraction collector
WARNING
UniNet cable. Only use UniNet cables delivered or approved by
Cytiva.
Operation
CAUTION
Risk of breaking test vials. Do not use excessive force to press
vials with incorrect dimensions into the fraction collector. Glass
vials may break and cause injuries.
CAUTION
Avoid spillage and overflow. Make sure that the equipment is
prepared according to the settings in the method to be run.
CAUTION
Replacement of the fraction collector bowl in Fraction
collector F9-R. Do not replace the bowl during a run.
2 Safety instructions
2.1 Safety precautions
Fraction collector F9-R Operating Instructions 29656880 AA 11

CAUTION
Replacing tubes. Do not replace the tubes in the fraction
collector during a run.
Maintenance
CAUTION
Electrical shock hazard. All repairs should be done by service
personnel authorized by Cytiva. Do not open any covers or replace
parts unless specifically stated in the user documentation.
CAUTION
Disconnect power. Always switch off power to Fraction collector
F9-R, before cleaning any of its components, unless stated other-
wise in the user documentation. This is done by switching off the
chromatographic instrument that provides Fraction collector F9-R
with power.
CAUTION
Hazardous chemicals and biological agents. Before mainte-
nance, service and decommissioning, wash Fraction collector F9-R
with a neutral solution to make sure that any hazardous solvents
and biological agents have been flushed out from the fraction
collector.
CAUTION
Always use appropriate personal protective equipment when
decommissioning the equipment.
2 Safety instructions
2.1 Safety precautions
12 Fraction collector F9-R Operating Instructions 29656880 AA

CAUTION
Cleaning Fraction collector F9-R before decommissioning.
•Wipe Fraction collector F9-R with a damp tissue using a
cleaning agent so that no hazardous solvents or biological
agents remain on the surface.
•Perform a system CIP using a neutral solution. Make sure that
any hazardous solvents or biological agents are flushed out
from the fraction collector.
NOTICE
Cleaning. Keep Fraction collector F9-R dry and clean. Wipe regu-
larly with a soft damp tissue and, if necessary, a mild cleaning
agent. Let Fraction collector F9-R dry completely before use.
2 Safety instructions
2.1 Safety precautions
Fraction collector F9-R Operating Instructions 29656880 AA 13

2.2 Labels and symbols
Introduction
This section describes the product nameplate (label) and other safety and regulatory
labels attached to the product.
System label
The system label is located on the back of the equipment. The system label identifies
the equipment and shows electrical data, regulatory compliance, and warning
symbols.
Label information
The label contains information on the serial number, regulatory compliance symbols,
recycling symbols, and specifications.
The label information is explained in the table below.
Label text Meaning
Code no Fraction collector code number
Serial no Fraction collector serial number
Mfg Year Manufacturing year and month
Voltage Supply voltage
Max Power Max power consumption
Protection class Protection class, ingress protection according to IEC
60529
Warning! Read the Operating Instruction before
using the module. Do not open any covers or replace
parts unless specifically stated in the Operating
Instructions.
This symbol indicates that electrical and electronic
equipment must not be disposed of as unsorted
municipal waste and must be collected separately.
Please contact an authorized representative of the
manufacturer for information concerning the
decommissioning of equipment.
The fraction collector complies with applicable Euro-
pean directives
2 Safety instructions
2.2 Labels and symbols
14 Fraction collector F9-R Operating Instructions 29656880 AA

Label text Meaning
The equipment complies with the applicable require-
ments for Australia and New Zealand
2 Safety instructions
2.2 Labels and symbols
Fraction collector F9-R Operating Instructions 29656880 AA 15

2.3 Emergency procedures
Introduction
The ÄKTA instrument supplies the Fraction collector F9-R with power. This section
describes how to perform an emergency shutdown of the Fraction collector F9-R by
shutting down the ÄKTA instrument.
This section also describes the result in the event of power failure or network interrup-
tion.
For more information on how to shut down and restart the instrument, refer to the
Operating Instructions for the ÄKTA instrument.
Emergency shutdown
In an emergency situation, stop the run by either pausing the run or switching off the
instrument as described below:
If you want
to...
then...
pause the run •press the Pause button on the Instrument control panel:
Note:
The buttons on the Instrument control panel may be locked.
This is an option available in the System settings.
or
•click the Pause icon in UNICORN:
Result: All pumps in the instrument are stopped.
2 Safety instructions
2.3 Emergency procedures
16 Fraction collector F9-R Operating Instructions 29656880 AA

If you want
to...
then...
switch off the
instrument •press the Power switch to the 0 position, or
•disconnect the power cord from the wall socket.
Result: The run is interrupted immediately.
Note:
The sample and data may be lost as a result of switching off the
power.
Power failure
In case of a power failure or if communication is lost, the run is interrupted immediately
and all moving parts in the fraction collector are stopped.
2 Safety instructions
2.3 Emergency procedures
Fraction collector F9-R Operating Instructions 29656880 AA 17

3 Overview
About this chapter
This chapter gives an overview of Fraction collector F9-R and suitable disposables and
accessories to be used with the fraction collector.
In this chapter
Section See page
3.1 Function 19
3.2 Illustrations 20
3.3 Tubes 23
3 Overview
18 Fraction collector F9-R Operating Instructions 29656880 AA

3.1 Function
The fraction collector collects fractions from purification runs on ÄKTA go, ÄKTA pure,
and ÄKTA avant instruments.
The fraction collector can be used for:
•Fixed volume fractionation
•Peak fractionation
•Combined fixed volume fractionation and peak fractionation
•Collecting fractions in reversed phase separations using organic solvents
Fraction collector F9-R has the following function for reducing sample spill during frac-
tionation:
•Drop Sync
3 Overview
3.1 Function
Fraction collector F9-R Operating Instructions 29656880 AA 19

3.2 Illustrations
Introduction
This subsection provides illustrations of Fraction collector F9-R. The main features and
components are indicated.
3 Overview
3.2 Illustrations
20 Fraction collector F9-R Operating Instructions 29656880 AA