cytiva Amersham ImageQuant 800 User manual

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QUICK START GUIDE
Amersham ImageQuant 800
Turning system ON
1. Press the power button on the side of the Amersham™
ImageQuant™ instrument
2. Press the power button on the mini PC attached to the rear
of the system
Starting imaging process
1. After system initialization, enter password and click Login
2. ConrmCCD status on the bottom left corner of the screen
is green
3. Choose detection method from the Home screen to begin
• Options: Chemiluminescence, Colorimetric, and
Fluorescence
Utilities
• Scheduler is a booking tool to reserve the imager for a
specicdateandtime
• Image library allows access to the default save location
where images are stored with options to view, copy/move, or
delete images
• Use Settings to view, set, and change system settings,
if needed
Using the chemiluminescence
imaging mode
1. Under Select Exposure, choose settings (or choose Open
method if you have saved a method previously)
• Auto: Sets exposure time automatically for the entire
blot based on highest pixel intensity. There is an option to
choose Pre-capture to select an area of interest, which
calculates optimal exposure time for the selected area.
• Manual: Enter exposure time manually
• Time series: Perform a series of exposures (maximum of
50). Signal is cumulated for each image if Cumulative is ON.
Toggle Cumulative OFF if summed images are not needed.
2. Click Start to begin imaging
Note Use the black tray with white insert if using for colorimetric marker. Chemiluminescence
detection is available in both upper and lower tray positions.

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Selecting SNOW exposure mode
Use SNOW exposure mode to extend dynamic range and
automatically detect both high and low abundance proteins at
the same time without saturation and with reduced noise.
1. After pre-capture, select the region of interest and background
2. Toggle Auto stop ON (recommended)
• Signal-to-noise ratio (S/N) improvement for image will be
visible in real time on the screen and exposure is stopped
automatically when optimum S/N is reached
Note The SNOW exposure option is available for both Chemiluminescence and Fluorescence
detection modes.
Advanced settings
• Binning: Default binning is pre-set. Use slider to increase or
decrease binning.
• Fluorescence multiplex: Useforimagingadditionaluor
channels along with chemi exposure (only available in Auto
and Manual exposure modes)
• Capture area: Default capture area is pre-selected. Choose
other area if needed.
Colorimetric
1. Choose Gel documentation or OD measurement. If
OD measurement is selected, intensity is converted to
ODvalueandsavedasacalibratedle.
Note For Gel documentation, use the black tray with white insert, and for OD measurement,
use the glass tray.
Fluorescence
1. Choose exposure type and select up to three channels.
Customltersanddyescanbeaddedifneededfrom
Settings menu.
Note Use black tray WITHOUT white insert for Fluorescence exposures. Fluorescence is
available in lower tray position only.
Exiting the system
1. Click the icon from the top right corner on the
Home screen
• Select Shut down to turn the instrument and PC off
completely (DO NOT press the power button to turn
system off)
• Minimize application gives you access to Windows®.
Returntocontrolsoftwarefromthetaskbarwhennished.
• Select Logout to leave the system ON but exit your account
and lock the system from unauthorized use