DNS AS-410 User manual

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AS-410
Operation Manual
Auto Trimming Unit
2017/8/21
Ver. １.00

２
Overview
This option enables the System to perform rough-cutting (trimming) while automatically monitoring the
exposure of the tissue on the sample block surface via a CCD camera under two LED lights (incident
and reflective). The System continues trimming until tissue is exposed for the predetermined tissue
exposure ratio (%). The photographed image is sent to the attached PC. The analysis processing and
judgment are done by automatic surface analysis software.
In order to use the Automatic Trimming Function, it is necessary to set up the conditions on the AS -
410 operation screen, the Trimming Setting screen, and the automatic surface analysis software. In
case of sectioning using this function, this setting is reflected in all the set blocks.
Incident LED light
CCD camera
Reflective LED light
Sample
Microtome blade Tissue
Paraffin-embedded tissue block (sample)

３
Device Configuration
Reflective Light
Auto Trimming Unit
photographing area
Diffused lighting
CCD camera

４
１．How to set
1－1．Setting on AS-410
Auto Surface Detection application detects the sample surface by following procedure.
①Apply Surface Cut (rough cut) to eliminate unevenness of the block surface.
②From the top surface of the block, the area of the block surface and the tissue image were taken.
Analyze and calculate the maximum tissue embedding area from the image.
③Initial trimming (roughing) is performed in order to expose the tissue surface to the block surface.
④Reflected light illuminates the surface. Tissue image exposed on the surface is photographed, and
the tissue surface is analyzed from the image and calculated.
⑤With the calculated maximum tissue embedding area as 100, the proportion of the tissue surface
exposed on the surface calculated in ④is displayed as the surface detection rate (%). The
measurement result is equal to or higher than the preferred set surface detection rate after the
roughing, sample preparation operation starts. If the exposed area ratio did not reach to the set
surface detection rate, the system repeats the rough cutting and measuring.
Note
・Tilting mechanism does not work with the Auto Trimming function.
・Samples in following conditions are not suitable for Auto Trimming function.
1. Color of the tissue specimen similar to the color of paraffin or paraffin
casette.
2. Tissue surface is not parallel to paraffin surface.
3. Embedded tissue thickness is less than 5mm.
4. Multiple tissues are embedded at different depth in single paraffin block.
5. Large gap between the tissue size observed from upper side and surfaced
tissue size. Exmaple: tube form
① Surface Cutting
② Capturing the
whole tissue image
③ Initial Trimming
④ Surfaced
area
⑤ Calculate the surface
detection rate (%).

５
1-1-1．Setting the Main Body
Set the conditions for the Auto Trimming function using the operation panel of the main
unit.
Setting Auto Trimming
Chose “MAINTENANCE” on the MAIN MENU screen.
①Chose “AUTO TRIMMING” on the MAINTENANCE screen.
When Auto trimming setting screen appears, enter the values referring to the Table 1.
MAINTENANCE screen
MAIN MENU screen
AUTO TRIM SETTING screen
Note
・Set the amount which eliminates unevenness for the Surface Cut amount.
・Make the initial setting for the Initial Trimming 100µm to start sectioning operation first. Confirm
the Auto Trimming condition and apply adjustment when necessary.
・Fort the Max. Vol, make the initial setting 500µm to start sectioning and confirm the
condition of the Auto Trimming, and apply adjustments if necessary.
・Make the initial setting value 70% and confirm the condition after the Auto Trimming, apply
adjustments if necessary.
表面カット量については、表面に凹凸が無くなる厚さを設定してください。

６
Chart 1. Auto Trimming Setting items
Basic auto trimming setting
On-screen item Description
Setting range
Surface detection ratio (Threshold)
(%)
Size of tissue specimen on paraffin surface compare to
the entire tissue size. 1 to 99
1st Thickness
Thickness of 1st surface trimming per single slicing.
3 to 30
2nd Thickness
Thickness of 2nd initial trimming per single slicing.
3 to 30
Sample Size
Sample block surface size
Reguler: 24x37mm to 16x16mm Small :15x15mm to
under
―
Surface trimming (1st trimming) (μm)
Amount of rough-cut before detecting entire tissue size. 0 to 999
Initial trimming (2nd trimming) (μm)
Amount of rough cut before detecting tissue size on
paraffin surface.
1 to 999
Retry count
Maximum number of the retry-trimming.
1 to 99
Maximum volume (thickness)
Max. limit of thickness of rough-cut during auto trimming
function.
―
Wait limit
In case of failing to reach threshold after auto trimming,
the machine will:
On: make tissue slides Off: skip slide sectioning
―
Trimming rate (%) The amount of additional trimming (rough-cut) if the
surface detection ratio does not exceeded the
threshold. The rough-cut volume is determined by the
increased volume (δ Threshold).
1 to 99
1 to 999
- 99 to 99
Humidification treatment
On-screen item Description
Setting range
Humidifying time
Length of humidification before measuring the tissue size
on paraffin surface.
0 to 999
Auto trimming manual check mode
On-screen item Description
Setting range
ON,OFF
ON: Apply Auto Trimming to all sample blocks first,
check, then start sectioning of the blocks. OFF: Apply
Auto Trimming and sectioning to each block one by one.
Add trimming (μm)
Amount of additional trimming after checking the image
on Auto Trimming screen. 0 to 999
Blade Change
Number of blocks to apply Auto Trimming per blade

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１－２．Setting the Auto Trimming Analysis Software
1－2－1．How to install the Auto Trimming Analysis software
（１） Open the AsScan_9 folder.
（２）Click
（３）Press Next (N) when the AsScan setup wizard appears.
（４）Choose the folder to install the software then
click next.
（５）Click Next on the installation conformation screen
when appeared.
。
（６）Click Close when the installation completion screen
appered.
（７）Open the installed folder（\KURABO\AsScan）, copy
BASEIMAGE、BASEIMAGED, and BASEIMAGER from
The AsScan_9 folder.
（8） Right click on the AsScan icon, choose Properties,
and check on “Run this program as an administrator.”

８
1－2－2．Setting device
１）Click “AsScan”icon on the desktop.
２）Check if Auto-Trimming analysis software opens normally.
３）Press “Abort” on the main menu of the analysis software.
４）Press the Save Directory button(①) and choose where to
save the examination images. （ex：Ｃ:\AS-400\work）
５）Press Parameter Setting button(②).
６）Enter the Password （71953）.
7）Set the examination setting parameter referring
to the list below dingly to the list below.
Item１ Item 2 Value
Examination
Area
(Entire area)
GAIN
Gain 350
White Balance B 43
White Balance R 56
Tissue area
(Tissue Size)
GAIN
Gain 277
White Balance B 43
White Balance R 56
Surface Area
(Tissue Surface)
GAIN
Gain 300
White Balance B Auto
Test
(Test)
GAIN
Gain 350
White Balance B Auto
Fig.2 main menu (PC)
Fig.1 Password input screen
Fig.3 Parameter setting Screen
①
②

９
8）Press Device button
Check the below listed items.
① If [Serial Number] is consistent with the Serial
Number with camera.
② If [Video Format] is BY8（640×480 VGA）.
③ If Frame Rate(FPS) is set with “30.0003.”
9）Press ‟Properties.”
10）Check the settings for Color, Exposure, and Image.
① Color
② Exposure
③ Image
11）Check the settings and press
[Update]→[OK] to finish.
Hue
180
Saturation 128
White Balance Red 56
White Balance Blue 43
Brightness
0
Saturation
300
Exposure
1/16 sec
Auto Reference
128
Auto Max Value
Auto
Image
100
Fig. 4 Device Setting screen
Fig. 5 Device Setting screen (color)
Fig. 6 Device Setting screen (Exposure)
Fig. 7 Device Setting screen (Image)

１０
1－2－３．３．Shooting position and light quantity adjustment
１）Set the block for adjustment in the device.
２）On the main unit operation panel, press “Main Menu”
→“Maintenance”→“Maker Maintenance”and input
password, press“Device
Setting,”and confirm that the Auto-Trimming is “ON.”
３）On the main unit operation panel, press “Main Menu”
→“Maintenance”→”Manual Operation” → “Sample
Feeding” and place the block for adjustment on
the Sample Stage.
４）Ｃhose“Manual Operation”“Sample Holder”to detect the level then detect the height.
Move the right and left axis (X) position to the surface detection position as well as the
up and down axis (Z).
５）Press LIVE button on the Menu screen.
６）Adjust the camera up & down and right & left position as viewing the screen.
７）Choose “Manual Operation” →”Auto-trimming” to move the reflected illumination to the
right and turn ON the light. Make certain to turn OFF the internal light for the device
(switch located on the front door).
８）Press the blue circles to check the amount of
light at the upper 2 points and the lower 2 points
and the central point on the block.
９）Adjust the aperture of the camera if amount
of detected blue light at the five points is not
between 90 ～110.
If there is a significant irregularity of lights,
check the position of the illumination position and
angle.
10） Start the Parameter Setting on PC and
press camera test “Reflected” button.
Fig.9 main menu PC (2)
Fig.11 main menu PC (1)
Fig.10 main menu PC (3)