Knauer Azura CM 2.1S User manual

Conductivity Monitor CM 2.1S
Instructions
Document No. V6710
HPLCHPLC

Note: For your own safety, read the instructions and observe the warn-
ings and safety information on the device and in the instructions.
Keep the instructions for future reference.
Note: In case you require this instruction in another language, please
submit your request including the corresponding document number via
e-mail or fax to KNAUER.
Do you have questions about the installation or the operation of your
instrument or software?
International Support:
Contact your local KNAUER partner for support:
www.knauer.net/en/Support/Distributors-worldwide
Support in Germany
(Austria & Switzerland on case-to-case basis):
Phone: [phone] (workdays 9-17h CET)
Fax : [phone]
Email: [e-mail]
KNAUER Wissenschaftliche Geräte GmbH
Hegauer Weg 38
14163 Berlin
Germany
Phone: [phone]
Fax: [phone]
Internet: www.knauer.net
E-Mail: [e-mail]
Document number: V6710
Version number: 4.0
Release date: 2021/05/03
Translation of the original edition
The information in this document is subject to change
without prior notice. For the latest version of the instructions,
visit our website: www.knauer.net/library
The printed versions of our instructions are printed according to
Blue Angel standards (www.blauer-engel.de/en/uz195).
This document contains confidential information and may not be
reproduced without written consent of KNAUER Wissenschaftliche
Geräte GmbH.
© KNAUER Wissenschaftliche Geräte GmbH 2021
All rights reserved.
AZURA® is a registered trademark of
KNAUER Wissenschaftliche Geräte GmbH.
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AZURA® Conductivity Monitor CM 2.1S Instructions, V6710
Table of contents
1. General ..............................................................................1
1.1 About these instructions..........................................................1
1.2 Signal words ....................................................................1
1.3 Additional typographical conventions ..............................................1
1.4 Legal information ................................................................2
1.4.1 Liability limitation .........................................................2
1.4.2 Transport damage ........................................................2
1.4.3 Warranty conditions .......................................................2
1.4.4 Warranty seal .............................................................2
1.4.5 Declaration of conformity ..................................................2
2. Basic safety instructions ................................................................3
2.1 Intended use....................................................................3
2.1.1 Operating ranges .........................................................3
2.1.2 Foreseeable misuse .......................................................3
2.2 User qualification ................................................................4
2.3 Operator responsibility...........................................................4
2.4 Personal safety equipment ........................................................4
2.5 Safety features on the device ......................................................5
2.6 Working with solvents ............................................................5
2.6.1 General requirements .....................................................5
2.6.2 Contamination by health-threatening solvents ................................5
2.6.3 Avoiding leakage .........................................................5
2.7 Specific environments............................................................6
2.7.1 Earthquake-endangered areas..............................................6
2.7.2 Explosive environment.....................................................6
2.7.3 Cooling room ............................................................6
2.7.4 Wet room ................................................................6
2.8 Maintenance, care and repair .....................................................6
2.9 Service request form and decontamination report ...................................7
3. Product information ...................................................................7
3.1 Features ........................................................................7
3.2 Device views ....................................................................7
3.3 Symbols and signs ...............................................................8
3.4 Scope of delivery ................................................................8

AZURA® Conductivity Monitor CM 2.1S Instructions, V6710
3.5 Eluents/buers ..................................................................9
3.5.1 Suitable eluents/buers....................................................9
3.5.2 Unsuitable eluents/buers ................................................10
4. Installation...........................................................................10
4.1 Setup .........................................................................10
4.1.1 Location requirements ....................................................10
4.1.2 Unpacking ..............................................................11
4.1.3 Integration into the FPLC/HPLC System .....................................11
4.2 CM flow cell ...................................................................12
4.2.1 Attaching CM flow cell to conductivity monitor...............................13
4.2.2 Connecting the capillaries of the CM flow cell ...............................13
4.3 pH monitor (optional) ...........................................................14
4.3.1 Assembling pH monitor and attaching to conductivity monitor.................14
4.3.2 Connecting pH monitor to capillaries .......................................15
4.4 Connecting the device to the computer ...........................................16
4.4.1 Connecting the device to the computer via LAN .............................16
4.4.2 Configuring the LAN settings ..............................................16
4.4.3 Connecting devices with LAN..............................................16
4.4.4 Configuring the router ....................................................17
4.4.5 Integrating the LAN into a company network ................................17
4.4.6 Controlling several systems separately in a LAN..............................18
4.4.7 Analog output ...........................................................18
4.5 Setting IP addresses via software .................................................18
4.5.1 Firmware Wizard: Setting a static IP address .................................19
4.5.2 Firmware Wizard: Setting a dynamic IP address ..............................20
4.6 Power connection ..............................................................20
5. Operation ...........................................................................21
5.1 Initial start-up ..................................................................21
5.2 Switch on ......................................................................21
5.2.1 Operating elements ......................................................22
5.2.2 Menu items and factory settings ...........................................22
5.3 Selecting the interface ..........................................................23
5.4 Checking the operating hours....................................................24
5.5 Activating/setting the temperature compensation ..................................24
5.6 Adjusting the calibration function.................................................25
5.7 Specifying the oset value .......................................................26
5.8 Calibrating the pH electrode .....................................................27

AZURA® Conductivity Monitor CM 2.1S Instructions, V6710
5.9 Switching o device ............................................................28
6. Functionality tests ....................................................................29
7. Troubleshooting......................................................................30
7.1 LAN...........................................................................30
7.2 Possible problems and solutions..................................................31
8. Maintenance and care ................................................................32
8.1 Maintenance contract ...........................................................32
8.2 Cleaning the device.............................................................32
8.3 Flushing and cleaning the CM flow cell ............................................32
8.4 Replacing the CM flow cell.......................................................33
8.5 Flushing and cleaning the pH monitor.............................................34
8.5.1 Cleaning pH electrode....................................................34
8.5.2 Cleaning pH flow cell .....................................................34
8.6 Replacing pH electrode .........................................................35
9. Transportation and storage ............................................................35
9.1 Preparing wetted components ...................................................35
9.2 Disassembling the device........................................................36
9.2.1 Disconnecting the power supply ...........................................36
9.2.2 Removing the CM flow cell ................................................36
9.2.3 pH-Monitor abbauen .....................................................36
9.3 Storing the device ..............................................................36
9.3.1 Storage of the conductivity monitor and the CM flow cell......................36
9.3.2 Storage of the pH monitor ................................................37
10. Disposal.............................................................................37
10.1 AVV marking in Germany ........................................................37
10.2 WEEE registration ..............................................................37
10.3 Solvents and other operating materials ............................................37
11. Technical data........................................................................38
12. Chemical compatibility of wetted materials..............................................39
12.1 General .......................................................................39
12.2 Plastics ........................................................................39
12.3 Non-metals ....................................................................41
12.4 Metals.........................................................................42
13. Repeat orders........................................................................43

General 1
AZURA® Conductivity Monitor CM 2.1S Instructions, V6710
1. General
1.1 About these instructions
These operating instructions enable the safe and ecient operation of
the device. The user must have carefully read and understood these
operating instructions before starting any work.
The basic prerequisite for safe operation is compliance with all safety
instructions (see chapter “2 Basic safety instructions”, p. 3). In addition
to the safety and warning instructions in these operating instructions, the
local accident prevention regulations and the national industrial safety
regulations apply.
These operating instructions are an integral part of the device. It must be
kept in the immediate vicinity of the device and accessible to the user at
all times.
You can download these and other instructions from the KNAUER web-
site: www.knauer.net/library
1.2 Signal words
Possible dangers related to the device are distinguished in personal and
material damages.
Symbol Meaning
DANGER (red) indicates a highly hazardous situa-
tion. If not avoided, it will result in death or serious
injury.
WARNING (orange) indicates a hazardous situa-
tion. If not avoided, it could result in death or
serious injury.
CAUTION (yellow) indicates a moderate hazardous
situation. If not avoided, it could result in minor or
moderate injury.
NOTICE (blue) is used to address issues which are
not related to physical injury.
1.3 Additional typographical conventions
Note: Specific information are prefixed with the word “Note” and an
information icon.
Note: This is an example.

2 General
AZURA® Conductivity Monitor CM 2.1S Instructions, V6710
1.4 Legal information
1.4.1 Liability limitation
The manufacturer is not liable for the following issues:
Non-compliance of these instructions
Non-observance of necessary safety precautions
Improper use
Operation of the device by unqualified personnel (see chapter “2.2
User qualification”, p. 4)
Use of non-approved spare parts
Technical changes by the user such as opening the device and unau-
thorized modifications
Violations of General Terms and Conditions (GTC)
1.4.2 Transport damage
The packaging of our devices provides the best possible protection
against transport damage. However, check the packaging for transport
damage. In case you notice any damage, inform the technical support
and the shipping company within three workdays.
1.4.3 Warranty conditions
For information on warranty please refer to our general terms and condi-
tions on the website: www.knauer.net/terms
1.4.4 Warranty seal
A blue or orange warranty seal is axed to some devices.
A blue seal is used by KNAUER’s Manufacturing or Customer Support
for devices to be sold.
After repair, service technicians attach an orange seal onto the identi-
cal position.
After repair, the service technician axes an orange seal in the same
place. If unauthorised persons tamper with the device or if the seal is
damaged, the warranty will lapse.
1.4.5 Declaration of conformity
The declaration of conformity is enclosed as a separate document with
the product and can be obtained online:
www.knauer.net/en/Support/Declarations-of-conformity

Basic safety instructions 3
AZURA® Conductivity Monitor CM 2.1S Instructions, V6710
2. Basic safety instructions
The device has been developed and constructed in such a way that haz-
ards arising from its intended use are largely excluded. Nevertheless, the
following safety instructions must be observed in order to exclude residu-
al hazards.
2.1 Intended use
Note: Only use the device for applications that fall within the range of
the intended use. Otherwise, the protective and safety equipment of the
device could fail.
The conductivity monitor CM 2.1S measures the electrical resistance of a
solution and indicates its conductivity. For example, the change in con-
ductivity during a programmed salt or buer gradient - as used in FPLC
and HPLC - can be followed.
The monitor consists of a processing device and a flow cell, and can addi-
tionally be used with a pH monitor. The pH flow cell and the pH electrode
must be ordered separately. In case you are not using the pH monitor,
you have to protect the connector on the device front with a with a BNC
blind plug that comes with the delivery.
The monitor is used for salinity gradient monitoring in the following
areas:
Biochromatography
Process analysis
Biochemical analysis
Pharmaceutical analysis
Environmental analysis
2.1.1 Operating ranges
The device is intended to be used indoors for chromatographic
applications.
2.1.2 Foreseeable misuse
Refrain from the use of the device for the following purposes or
conditions:
Medical purposes. The device is not approved as a medical product.
Operating outdoors. Otherwise, the manufacturer does not guarantee
the functionality and safety of the device.
Operation in potentially explosive areas without special and addition-
al explosion protection. Contact the KNAUER Customer Support for
more information.
Description
Operating Range

4 Basic safety instructions
AZURA® Conductivity Monitor CM 2.1S Instructions, V6710
2.2 User qualification
The users are qualified to handle the device if all of the following points
apply:
They have at least a basic knowledge of liquid chromatography.
They have knowledge about the properties of the used solvents and
their health risks.
They are trained for the special tasks and activities in the laboratory
and know the relevant standards and regulations.
Due to their technical training and experience, they can under-
stand and carry out all the work described in the operating instruc-
tions on the instrument and recognize and avoid possible dangers
independently.
Their ability to react is not impaired by the consumption of drugs,
alcohol or medication.
They have participated in the installation of an instrument or training
by KNAUER or an authorized company.
If users do not meet these qualifications, they must inform their
supervisors.
2.3 Operator responsibility
The operator is any person who operates the device himself or leaves it to
a third party for use and who bears the legal product responsibility for the
protection of the user or third parties during operation.
The obligations of the operator are listed below:
Know and follow the applicable work safety regulations
Identify hazards arising from the working conditions at the place of use
in a risk assessment.
Set up operating instructions for the operation of the device.
Regularly check whether the operating instructions correspond to the
current status of the regulations.
Clearly regulate and specify responsibilities for installation, operation,
troubleshooting, maintenance and cleaning and set clear rules
Ensure that all personnel who work with the device have read and
understood these operating instructions
Train the personnel who work with the device at regular intervals and
inform them about the dangers.
Provide the necessary safety equipment to the employees working
with the unit (see section below).
2.4 Personal safety equipment
The protective measures required in the laboratory must be observed
and the following protective clothing worn during all work on the device:
Safety glasses with side protection
Protective gloves in accordance with the prevailing ambient conditions
and used solvents (e.g. heat, cold, protection against chemicals)
Lab coat

Basic safety instructions 5
AZURA® Conductivity Monitor CM 2.1S Instructions, V6710
Personalised protective safety equipment which is specified in the
particular laboratory.
2.5 Safety features on the device
Power switch: Devices of the AZURA® L series may be switched o
using the power switch (toggle switch on the back side of housing) at
any time, this causes no damage to the device. To switch o devices of
the AZURA® S series, remove the plug from the power socket.
2.6 Working with solvents
2.6.1 General requirements
The user is trained for handling dierent solvents.
Note recommended solvents and concentrations in these instructions
in order to avoid personal injury or damage to the device. For exam-
ple, certain chemicals may cause PEEK capillaries to swell or burst (see
chapter “12 Chemical compatibility of wetted materials”, p. 39).
Note that organic solvents are toxic above a certain concentration. For
handling hazardous solvents see the following section.
Mobile phases and samples may contain volatile or combustible sol-
vents. Avoid the accumulation of these substances. Ensure good ven-
tilation of the installation site. Avoid open flames and sparks. Do not
operate the instrument in the presence of flammable gases or vapors.
Only use solvents which do not self-ignite under given conditions. This
applies especially to the use of a thermostat where liquids could get
onto hot surfaces in the interior.
Degas solvents before use and observe their purity.
2.6.2 Contamination by health-threatening solvents
Contamination with toxic, infectious or radioactive substances poses
a hazard for all persons involved during operation, repair, sale, and dis-
posal of a device.
All contaminated devices must be properly decontaminated by a spe-
cialist company or the operating company before they can be recom-
missioned, repaired, sold, or disposed (see chapter “10 Disposal”, p.
37).
2.6.3 Avoiding leakage
Risk of electrical shock or short circuit if solvents or other liquids leak into
the interior of the device. You can avoid a leakage through the following
measures:
Tightness: Visually check the device or system regularly for leaks.
Solvent tray: The use of a solvent tray prevents liquids get from the
bottles into the inside of the device.
Eluent lines: Install capillaries and hoses in such a way that, in case of a
leak, liquids cannot get into the interior of the devices underneath.
In case of leakage: Switch o the system. Only take the device into
operation if the cause of the leak has been resolved
(see chapter “8 Maintenance and care”, p. 32).

6 Basic safety instructions
AZURA® Conductivity Monitor CM 2.1S Instructions, V6710
2.7 Specific environments
2 .7.1 Earthquake-endangered areas
In earthquake-endangered areas, do not stack more than 3 devices on
top of each other. Otherwise there is risk of injury due to falling devices
or loose parts.
2 .7. 2 Explosive environment
Never use the system in potentially explosive atmospheres without
appropriate protective equipment. For more information, contact the
KNAUER Customer Support.
2 .7. 3 Cooling room
You may operate the device in a cooling room. To prevent condensation,
note the following instructions:
Allow the device to acclimatize for min. 3 hours before taking it into
operation.
After taking into operation, the device should stay switched on.
Avoid temperature fluctuations.
2 .7. 4 Wet room
The device must not be operated in wet rooms.
2.8 Maintenance, care and repair
Avoiding electric shock: Before performing any maintenance and ser-
vice work, disconnect the device from the power supply.
Tools: Use only tools recommended or prescribed by the
manufacturer.
Spare parts and accessories: Only use original parts and accessories
made by KNAUER or a company authorized by KNAUER.
PEEK fittings: Use PEEK fittings only for a single port or brand-new
PEEK fittings in order to avoid dead volume or not exactly fitting
connections.
Column care: Follow KNAUER or other manufacturer’s instructions on
caring for the columns (see www.knauer.net/columncare)
Used capillaries: Do not use any used capillaries elsewhere in the
system in order to avoid dead volumes, not exactly fitting connections
and spreading contamination.
Safety features: The device may only be opened by the KNAUER Cus-
tomer Support of KNAUER or any company authorized by KNAUER
(see chapter “1.4.1 Liability limitation”, p. 2).
For more information visit the KNAUER website:
www.knauer.net/hplc-troubleshooting

Product information 7
AZURA® Conductivity Monitor CM 2.1S Instructions, V6710
2.9 Service request form and decontamination
report
Devices which are shipped without the completed document “Service
request form and decontamination report” will not be repaired. If you
would like to return a device to KNAUER, make sure to enclose the com-
pleted document: www.knauer.net/servicerequest.
3. Product information
3.1 Features
The conductivity monitor is non-contacting. This means that the liquid flo-
wing through the device does not come into contact with any conductive
or current-carrying material at any point. This design makes the
CM 2.1S a practically maintenance-free device, since unlike other tech-
niques, electrochemical reactions are prevented. The tracer is made of
aluminum and PEEK.
Flow cells with flow rate ranges between 0 –10ml/min and
10 –100ml/min are available. 1/16“ capillaries are part of the flow cell
and serve to connect the flow cell to the FPLC/HPLC system.
The pH monitor consists of a calomel pH electrode and a flow cell. You
can integrate the sensor into the system by mounting the pH flow cell
onto an AZURA® organizer. The pH measuring kit and the AZURA® orga-
nizer are not included in the scope of delivery and can be purchased
separately.
3.2 Device views
Legend:
1
Display
2
Arrow keys
3
Connection for
pH monitor (optional)
4
Connection for CM
flow cell
Fig. 1: Front view
Flow cells
pH monitor
2
1
3
4

8 Product information
AZURA® Conductivity Monitor CM 2.1S Instructions, V6710
Legend:
5
LAN connector
6
Analog ports
7
RS-232 connector
8
Power connection
9
Ground
Fig. 2: Rear view
3.3 Symbols and signs
The following symbols and labels are located on the device:
Symbol Description
Electrostatic discharge hazard, damages to
system, device, or components can occur.
Hazard due to high voltage.
A device or system marked with CE fulfills the
product specific requirements of European
directives. This is confirmed in a Declaration
of Conformity.
3.4 Scope of delivery
Note: Only use original parts and accessories made by the manufacturer
or a company authorized by the manufacturer.
AZURA® Conductivity monitor CM 2.1S
CM flow cell with screw fittings and tool
Instructions (V6710)
Mains power connection 110 V – 240 V, voltage output: 24V/60W
Power cable
CM 2.1S accessories kit
5
6
7
8
9

Product information 9
AZURA® Conductivity Monitor CM 2.1S Instructions, V6710
3.5 Eluents/buers
Refer to the following listing for eluent selection.
Even small quantities of other substances, such as additives, modifiers,
or salts can influence the durability of the materials. If there is any doubt,
contact the technical support of the manufacturer.
Note: The list of selected solvents was compiled based on research in
the pertinent literature and is only a recommendation by KNAUER.
3.5.1 Suitable eluents/buers
Eluents/buers suitable for CM flow cell and pH flow cell
Acetate buer solutions
Acetone
Benzene
Chloroform
Ethyl acetate
Ethanol
Formiate buer solution
Isopropanol
Potassium chloride solutions
Carbon dioxide
Methanol
Sodium chloride solutions
Phosphate buer solutions
Toluol
Ammoniated dilute solution
Dilute acetic acid
Dilute sodium hydroxide
Water
Eluents/buers suitable for pH electrode or glass electrodes in
general
Aqueous salt or buer solutions
Also observe the operating instructions of the ph electrode
for suitable eluents or contact the manufacturer.
CM flow cell and
pH flow cell
pH electrode

10 Installation
AZURA® Conductivity Monitor CM 2.1S Instructions, V6710
3.5.2 Unsuitable eluents/buers
Eluents/buers unsuitable for CM flow cell and pH flow cell
Acetonitril
Eluents/buers unsuitable for pH electrode or glass electrodes in
general
Media that can dissolve the glass surface, for example:
Hydrofluoric acid solutions
Acidified fluoride solutions
Concentrated alkalis
Media that can dehydrate the glass surface, for example:
Ethanol
Sulfuric acid
Purified or demineralized water is not suitable as a storage medium
Media with components that can adhere to the glass surface
Media with organic content
Note: Be sure to observe the separate storage regulations for
CM flow cell, pH flow cell and pH electrode.
4. Installation
4.1 Setup
When installing the device, please pay attention to the suitability of the
location according to the requirements. The setup requirements and a
description can be found in the following section.
4.1.1 Location requirements
Device defect
The device overheats at exposure to sunlight and insucient air
circulation. Device failures are very likely.
ÎSet up the device in such a way that it is protected against
exposure to direct sunlight.
ÎLeave room for air circulation: See paragraph „space
requirements“.
Sunlight: Protect the device against direct exposure to sunlight.
A/C system: Set up the device at a location not exposed to air drafts.
Vibration: Do not set up the device in the vicinity of other machines
that cause floor vibrations.
CM flow cell and
pH flow cell
pH electrode

Installation 11
AZURA® Conductivity Monitor CM 2.1S Instructions, V6710
4.1.2 Unpacking
There is no noticeable damage to the packaging.
Utility knife
Process
1. Set-up the package in such a way that you can read the label. Using
the utility knife, cut the adhesive tape and open the packaging.
2. Remove the foam insert. Take out the accessories kit and the
manual.
3. Open the accessories kit. Check the scope of delivery. In case any
parts are missing, contact the technical support.
4. Clasp the device at its side panels and lift it out of the packaging.
5. Remove the foam inserts from the device.
6. Check the device for signs of damage that occurred during trans-
port. In case you notice any damage, contact the technical support.
7. Set-up the device in its location.
8. Remove the protective foil.
Carefully store all packaging material.
4.1.3 Integration into the FPLC/HPLC System
Integrate the conductivity monitor into the FPLC/HPLC system. In case
you want to use the conductivity monitor together with an UV detector
and a pH monitor in one system, you should position the monitor behind
the UV detector and before the pH flow cell, as the pH monitor has a
maximum pressure stability of 5 bar.
Legend:
1
Solvent bottles
2
Solvent tray
3
Injection valve
4
Binary pump
5
Column
6
Flow cell of UV detector
7
CM flow cell
8
Conductivity monitor
AZURA® CM 2.1S
9
pH flow cell
bl
pH electrode
bm
AZURA® Organizer
bn Waste bottle
Fig. 3: Example: Connetion of a binary FPLC gradient system with a
UV detector
Prerequisites
Tools
Next steps
2
3
4
5
6
7
9
bm
bn
bl
8
1

12 Installation
AZURA® Conductivity Monitor CM 2.1S Instructions, V6710
4.2 CM flow cell
The CM flow cell is supplied with built-in capillaries that cannot be disas-
sembled. In the event that the capillary is clogged and cannot be cleaned
even by repeated flushing processes, the CM flow cell must be replaced.
There is a plug on the rear side of the CM flow cell, which is used to con-
nect the flow cell to the front side of the monitor.
Note: The analytical flow cell A4156 generates a certain back pressure
(approximately 1 bar at 1 ml/min). For minimal backpressure choose flow
cell A4157.
Legend:
1
Fitting
2
SST ferrule
3
PEEK ferrule
4
1/8"-1/16"-
reducer
5
1/16"-1/16"-coupling
6
Capillary
7
Indication of manufactu-
rer, part number, serial
number
8
Indication of recom-
mended operating
range
9
Drill holes for mounting
bl Screws
bm Plug
Fig. 4: CM flow cell: front with labeling, accessories
Fig. 5: CM flow cell: rear side with connector
7
4
bl
1
2
9
6
358
bm

Installation 13
AZURA® Conductivity Monitor CM 2.1S Instructions, V6710
4.2.1 Attaching CM flow cell to conductivity monitor
Device defect
Device damage possible.
ÎSwitch o the device before mounting/dismounting the flow
cell.
ÎObserve the notes in chapter „Replacing the CM flow cell“ on
page 33.
The device has been switched o.
Hexagon wrench, size 1.5 mm
Process Figure
1. Plug the CM flow cell onto the
socket 1on the front side of
the conductivity monitor.
2. Using the wrench, tighten the
2 outerscrews 2.
2
Connect the capillaries.
4.2.2 Connecting the capillaries of the CM flow cell
The device has been switched o.
The capillaries belong to the flow cell and can not be removed. To integ-
rate the flow cell into the FPLC/HPLC flow path, use the supplied cou-
plings to connect the capillaries.
INLET: Connect one capillary end of the CM flow cell to the column or
downstream detector.
OUTLET: Connect the other capillary end to the waste or intermediate
instruments such as pH electrode, pressure sensor, fraction collector,
etc.
Install the pH monitor at the connection on the front side of the monitor
(s. Fig.1, Nr. 3), or close the connection using the BNC blind plug sup-
plied in the scope of delivery.
Prerequisites
ToolsTools
1
Next steps
Prerequisites
Process
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14 Installation
AZURA® Conductivity Monitor CM 2.1S Instructions, V6710
4.3 pH monitor (optional)
The pH monitor is used to measure the pH value in aqueous solutions
such as buers. The pH monitor consists of a pH electrode and a pH flow
cell.
When flushing the monitor at high flowrates or using non-saline solvents,
the pH electrode should be excluded from the flowpath. If the pH electro-
de is not plugged into the socket of the CM 2.1S (s. Fig.1, Nr. 3), cover
the socket using the BNC blind plug.
Legend:
1
Locking ring
2
pH flow cell
3
pH electrode with
protective cap
4
BNC blind plug
Fig. 6: pH flow cell, pH electrode and BNC blind plug
4.3.1 Assembling pH monitor and attaching to conductivity
monitor
Process Figure
1. Screw the locking ring 1o
the pH flow cell and pull it over
the cable of the pH electrode
2.
2. Remove the protective cap 3
from the pH electrode.
3. Insert the pH electrode into
the pH flow cell and tighten
the locking ring.
4
2
1
3
2
1
3

Installation 15
AZURA® Conductivity Monitor CM 2.1S Instructions, V6710
Process Figure
4. Connect the plug of the elec-
trode to the socket 4on the
front side of the monitor.
Integrate the pH monitor into the FPLC/HPLC flow path by installing it
downstream of the conductivity monitor. You can do this by mounting
the pH monitor onto the AZURA® Organizer, which is placed on the
AZURA® Click (both sold separately) at the side of the system. For more
information see supplement “AZURA® Organizer (V6868)“.
Note: Make sure that the maximum backpressure of 5 bar is not
exceeded in this configuration.
4.3.2 Connecting pH monitor to capillaries
The pH monitor has been mounted to the monitor.
Connect the capillaries in the direction of the flow. Use bushings and
ferrules with flat bottoms. Do not use any tools to tighten the bushings.
Legend:
1
Inlet
1
Outlet
Fig. 7: Inlet und Outlet of the pH electrode
INLET: Connect the capillary coming from the conductivity monitor.
OUTLET: Connect the capillary which either go to waste, another
detector or the fraction collector.
Conduct the calibration of the pH electrode with 2 dierent pH calibrat-
ion buer solutions (e. g. 4 and 7, see chapter „Calibrating the pH electro-
de“ on page 27).
4
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