Emerson Rosemount 550pH User manual

Reference Manual
00809-0100-3550, Rev AA
March 2019
Rosemount™ 550pH
Single-use pH Sensor for Bioprocessing Applications

Essential instructions
Read this page before proceeding!
Emerson designs, manufactures, and tests its products to meet many national and international standards. Because these
instruments are sophisticated technical products, you must properly install, use, and maintain them to ensure they continue to
operate within their normal specifications. The following instructions must be adhered to and integrated into your safety program
when installing, using, and maintaining Emerson products. Failure to follow the proper instructions may cause any one of the
following situations to occur: loss of life, personal injury, property damage, damage to this instrument, and warranty invalidation.
•Read all instructions prior to installing, operating, and servicing the product.
•If this Reference Manual is not the correct one, call 1-800-999-9307 to request the correct Reference Manual. Save this
Reference Manual for future reference.
•If you do not understand any of the instructions, contact your Emerson representative for clarification.
•Follow all warnings, cautions, and instructions marked on and supplied with the product.
•Inform and educate your personnel in the proper installation, operation, and maintenance of the product.
•Install equipment as specified in the installation instructions of the appropriate Reference Manual and per applicable local and
national codes. Connect all products to the proper electrical and pressure sources.
•To ensure proper performance, use qualified personnel to install, operate, update, program, and maintain the product.
•When replacement parts are required, ensure that qualified people use replacement parts specified by Rosemount.
Unauthorized parts and procedures can affect the product's performance, place the safe operation of your process at risk, and
may result in fire, electrical hazards, or improper operation.
•Ensure that all equipment doors are closed and protective covers are in place, except when maintenance is being performed
by qualified people, to prevent electrical shock and personal injury.
2

Contents
Chapter 1 Sensor readiness............................................................................................................ 5
1.1 Storage and handling....................................................................................................................... 5
1.2 Specifications................................................................................................................................... 5
1.3 Dimensional drawings...................................................................................................................... 7
Chapter 2 Install the sensor............................................................................................................9
Chapter 3 Wire the sensor............................................................................................................13
Chapter 4 Calibrate...................................................................................................................... 17
4.1 One-point standardization based on a grab sample........................................................................ 17
Chapter 5 Troubleshoot............................................................................................................... 19
5.1 Faulty reading.................................................................................................................................19
5.2 Label information too small............................................................................................................ 19
Chapter 6 China RoHS table..........................................................................................................21
Chapter 7 EU Declaration of Conformity....................................................................................... 23
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Contents Reference Manual
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1 Sensor readiness
1.1 Storage and handling
Store the sensors attached to the single-use bioreactor until needed.
WARNING
Do not allow sensors to freeze or go below 39 °F (4° C).
Disposal
If the sensors have been contaminated, decontaminate prior to disposal. Please dispose
with other electrical and electronic waste per local guidelines. Declarations of Conformity
can be found at the end of this manual.
1.2 Specifications
Table 1-1: Rosemount™ 550pH Sensor Specifications
Specifications Materials and units
Operating temperature range 39 to 104 °F (4 to 40 °C)
Pressure range 0 to 30 Psig (0 to 2 bar)
pH range 2 to 12
Sensor drift Less than 0.005 pH drift per day in laboratory
conditions
Initial one-point standardization accuracy using
internal sensor buffer
Less than ±0.1 pH at pH 7
Shelf life 2 years at 77 °F (25 °C), expiration date on
sensor label
Measuring method
Potentiometry pH glass electrode and double-junction
reference electrode
Wetted materials
Body material ULTEM™/PEI (USP Class VI)
pH electrode Proprietary glass formulation
O-ring EPDM (USP Class VI)
Operations parameters
Process connection 1-in. barb fitting
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Table 1-1: Rosemount™ 550pH Sensor Specifications (continued)
Specifications Materials and units
Electrical connector Variopol (VP 8.0)
Sensitivity Greater than 57 mV / pH at 77 °F (25 °C)
Electrolyte Phosphate based (see SDS on product website)
Integral temperature sensor Pt-100
Gamma irradiation Up to 50 kGy
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1.3 Dimensional drawings
See Figure 1-1 for sensor dimensions
Figure 1-1: Rosemount 550pH A1M22E0VP000 Dimensional Drawing
A. VP8 cable connection
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2 Install the sensor
The sensor will be integrated into a 1-in. barb fitting, sealed by several zip tie connectors.
Note
Complete Step 1 through Step 3 before filling the single-use bioreactor with solution.
Procedure
1. Orient the sensor within 10 and 45 ° of horizontal.
See Figure 2-1.
Figure 2-1: Sensor Orientation
2. Wire the sensor to the transmitter and wait for the reading to stabilize.
See Wire the sensor for instructions on wiring the sensor to Rosemount™
transmitters.
3. Use the Calibration menu on the transmitter to go to Slope/offset setting.
Recommendation: Do not fill the bag with buffer or other solution until after 3.b to
confirm sensor functionality.
a) Input the factory calibrated slope on the sensor label into the "Slope at 25 C"
field and press ENTER. Press EXIT to go back to the Calibration menu.
Do not touch the value in the "Offset" field. The offset will change and be
shown after 3.b.
Note
To find the sensor slope and cal pH, scan the two-dimensional barcode on
the sensor label.
See Figure 2-2.
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Figure 2-2: Enter Data on Calibrate Screen
b) In the Calibration menu, go to the "Standardize (grab)" option. Press ENTER
at the installation prompt since the reading should already be stabilized.
Input the Cal pH on the sensor label into the "pH of grab sample" field to
complete the initial sensor standardization.
Note
Use pH sensor prior to expiration date.
See Figure 2-2.
4. Clip the zip tie, locking the clip in place. Remove the circular warning tag if it is still
present.
See Figure 2-3.
Figure 2-3: Clipped Zip Tie
5. Press the slide lock into the unlocked position.
The small lip of the lock should be the side that is pressed in. See Figure 2-4.
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Figure 2-4: Unlocked Slide Lock
Note
Before continuing to the next step, ensure the sensor is fully submerged in the
solution.
6. Use the plunger assembly to push the sensor into the activated position.
See Figure 2-5.
Figure 2-5: Activated Sensor
7. Press the slide lock into the locked position.
The small tab on the slide lock should be protruding out. See Figure 2-6.
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Figure 2-6: Locked Slide Lock
8. Install a zip tie into the slide lock to prevent the sensor from returning to storage
position.
See Figure 2-7.
Figure 2-7: Zip Tie
Postrequisites
After the start-up is complete, there is no additional work needed on the sensor unless a
one-point standardization is deemed necessary. See One-point standardization based on a
grab sample for this procedure using an off-line sample.
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3 Wire the sensor
Use the following wiring diagrams to wire the Rosemount™ 550pH Sensor to the
Rosemount 56, 1056, 1057, 1066, and 5081 Transmitters.
1. See Figure 3-1 to wire the Rosemount 550pH Sensor to the Rosemount 56, 1056, or
1057 Transmitter.
Figure 3-1: Wire the Rosemount 550pH Sensor to the Rosemount 56, 1056, or
1057 Transmitter
2. See Figure 3-2 to wire the Rosemount 550pH Sensor to the Rosemount 1066
Transmitter.
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Figure 3-2: Wire the Rosemount 550pH Sensor to the Rosemount 1066
Transmitter
3. See Figure 3-3 to wire the Rosemount 550pH Sensor to the Rosemount 5081
Transmitter.
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Figure 3-3: Wire the Rosemount 550pH Sensor to the Rosemount 5081
Transmitter
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4 Calibrate
4.1 One-point standardization based on a grab
sample
Complete the following steps to perform a one-point calibration against an off-line
sample. This procedure is written for a Rosemount ™ 56 transmitter.
Procedure
1. Press ENTER on the transmitter.
2. Select "S1 Measurement" or "S2 Measurement", depending on which sensor you
wish to standardize.
3. Select "Standardize (grab)" in the Calibration menu.
4. Once the pH reading of the sensor is stabilized, press ENTER to pass the installation
prompt.
5. Input the pH of the grab sample into the "pH of grab sample" field and press ENTER.
6. Press EXIT to complete the calibration.
The pH reading on the screen should now equal the pH of the grab sample.
Note
pH is a temperature dependent attribute. Please ensure off-line sample is at the same
temperature as the process, so that no errors in standardization occur.
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5 Troubleshoot
5.1 Faulty reading
The one-point standardization at startup has a faulty reading.
Recommended actions
1. Rotate sensor within sensor sleeve 90 degrees.
2. Repeat sensor standardization procedure.
5.2 Label information too small
The serial number, Cal pH, or slope are too small to read.
Recommended action
Scan the two-dimensional bar code on the sensor label to retrieve information.
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