Endress+Hauser Raman Rxn2 User manual

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Operating Instructions
Raman Rxn2
BA02151C/66/EN/04.23
70212038

Operating Instructions Raman Rxn2
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Raman Rxn2 Operating Instructions
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Table of Contents
1About this document............................ 4
1.1 Warnings..................................................................4
1.2 Symbols on the device.............................................4
1.3 U.S. export compliance ...........................................4
1.4 List of abbreviations................................................5
2Basic safety instructions...................... 7
2.1 Requirements for the personnel............................7
2.2 Intended use ............................................................7
2.3 Workplace safety.....................................................7
2.4 Operational safety...................................................7
2.5 Product safety..........................................................8
2.6 IT security.................................................................8
3Product description .............................. 9
3.1 The Raman Rxn2 analyzer .....................................9
3.2 Raman RunTime software overview ...................10
3.3 Product design .......................................................11
3.4 Probe connectors...................................................13
4Incoming product acceptance and
identification ......................................14
4.1 Incoming acceptance.............................................14
4.2 Scope of delivery....................................................15
4.3 Certificates and approvals ....................................15
5Installation..........................................16
5.1 Site requirements..................................................16
5.2 Initial analyzer setup ............................................17
6Electrical and I/O connections ..........23
6.1 Overview of rear panel electrical and I/O
connections............................................................23
7Commissioning................................... 24
7.1 Connectivity...........................................................24
7.2 Electrical and I/O connections.............................24
7.3 Interior of the Raman Rxn2 .................................31
7.4 Raman Rxn2 hardware components...................32
8Operation ............................................ 35
8.1 Raman RunTime embedded software.................35
8.2 Initial Raman RunTime setup ..............................35
8.3 Calibration and verification .................................36
9Diagnostics and troubleshooting...... 38
9.1 Warnings and errors.............................................38
9.2 Raman Rxn2 system and power loss...................40
10 Maintenance....................................... 41
10.1 Optimization..........................................................41
10.2 Servicing the Raman Rxn2 analyzer ...................42
11 Repair .................................................. 45
11.1 Servicing and spare parts.....................................45
12 Technical data .................................... 46
12.1 Specifications.........................................................46
12.2 Certifications .........................................................47
13 Supplementary documentation ........ 48
14 Index.................................................... 49

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1About this document
1.1 Warnings
Structure of Information
Meaning
WARNING
Causes (/consequences)
Consequences of non-compliance (if applicable)
‣ Corrective action
This symbol alerts you to a dangerous situation. Failure to avoid the
dangerous situation can result in a fatal or serious injury.
CAUTION
Causes (/consequences)
Consequences of non-compliance (if applicable)
‣ Corrective action
This symbol alerts you to a dangerous situation. Failure to avoid this situation
can result in minor or more serious injuries.
NOTICE
Cause/situation
Consequences of non-compliance (if applicable)
‣ Action/note
This symbol alerts you to situations which may result in damage to property.
1.2 Symbols on the device
Symbol
Description
The Laser Radiation symbol is used to alert the user to the danger of exposure to hazardous visible laser radiation
when using the Raman Rxn2 system.
The High Voltage symbol that alerts people to the presence of electric potential large enough to cause injury or
damage. In certain industries, high voltage refers to voltage above a certain threshold. Equipment and conductors that
carry high voltage warrant special safety requirements and procedures.
The CSA Certification Mark indicates that the product was tested against and met the applicable North American
standards requirements.
The WEEE symbol indicates that the product should not be discarded as unsorted waste but must be sent to separate
collection facilities for recovery and recycling.
The CE Marking indicates conformity with health, safety, and environmental protection standards for products sold
within the European economic area (EEA).
1.3 U.S. export compliance
The policy of Endress+Hauser is strict compliance with U.S. export control laws as detailed on the website of the
Bureau of Industry and Security at the U.S. Department of Commerce.

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1.4 List of abbreviations
Term
Description
AC
alternating current
ALT
alternate
ANSI
American National Standards Institute
ATX
advanced technology extended
ATEX
atmosphere explosible
AWG
American wire gauge
°C
Celsius
CAL
calibration
CDRH
Center for Devices and Radiological Health
CFR
Code of Federal Regulations
cm
centimeter
COLL
collection
CSM
calibration switching module
CSV
comma separated value
DC
direct current
EMC
electromagnetic compatibility
EO
electro-optical
EPL
equipment protection level
EU
European Union
EXC
excitation
°F
Fahrenheit
FC
ferrule connector
GLP
good laboratory practice
GMP
good manufacturing practice
HCA
Raman calibration accessory
Hz
hertz
IEC
International Electrotechnical Commission
INTLK
interlock
IP
internet protocol
IPA
isopropyl alcohol
IR
infrared
IS
intrinsically safe
LED
light emitting diode
LVD
low voltage directive
mm
millimeter
MT
mechanical transfer
mW
milliwatt

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Term
Description
NA
numerical aperture
NAT
network address translation
nm
nanometer
OPC
Open Platform Communications
OPC UA
OPC Unified Architecture
PAT
process analytical technology
PCM
power control module
PDF
portable document format
p/n
part number
QbD
quality by design
RTU
remote terminal unit
SPC
spectrum
TCP
transmission control protocol
UPS
uninterruptible power supply
USB
universal serial bus
V
volt
W
watt
WEEE
waste electrical and electronic equipment

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2Basic safety instructions
Read this section carefully to avoid danger to individuals or the facility. Additional laser safety information and
hazardous area certification and safety instructions are contained in the Raman Rxn2 Safety Instructions
(XA02700C). See Supplementary documentation → .
2.1 Requirements for the personnel
•Installation, commissioning, operation, and maintenance of the measuring system may be carried out only by
specially trained technical personnel.
•Technical personnel must be authorized by the plant operator to carry out the specified activities.
•Electrical connections may be performed only by an electrical technician.
•Technical personnel must have read and understood these Operating Instructions and must follow the
instructions contained herein.
•Faults at the measuring point may only be rectified by authorized trained personnel. Repairs not described in
this document must be carried out only directly at the manufacturer's site or by the service organization.
2.2 Intended use
The Raman Rxn2 analyzer is designed for use in chemical composition measurements of solids, liquids, or turbid
media in a laboratory or process development environment.
The Raman Rxn2 is particularly suited for use in the following applications:
•Endpoint monitoring of chemical reactions
•Crystallinity monitoring of solid materials
•Critical process parameter monitoring and control in upstream cell culture or fermentation bioprocesses
•Molecular structure and composition of plant-based proteins, dairy solids, and cell-based foods
•Small molecule pharmaceutical polymorph identification and monitoring
Use of the device for any purpose other than that described poses a threat to the safety of people and of the entire
measuring system and is not permitted.
2.3 Workplace safety
•Do not use the Raman Rxn2 for anything other than its intended use.
•Do not drape the power cord over counters or on hot surfaces, or in areas where damage to the integrity of the
power cord may occur.
•Do not open the enclosure of the Raman Rxn2.
•Do not look directly into the laser beam.
•Do not allow emitted laser light to reflect off mirrored or shiny surfaces in an uncontrolled way.
•Minimize the presence of shiny surfaces in the working area and always use a laser beam block to prevent
uncontrolled transmission of the laser light.
•Do not leave unused probes uncapped or unblocked while they are still attached to the analyzer.
2.4 Operational safety
Before commissioning the entire measuring point:
1. Verify that all connections are correct.
2. Ensure that electrical cables and optical fiber connections are not damaged.
3. Do not operate damaged products. Protect them against unintentional operation.
4. Label damaged products as defective.

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During operation:
1. If faults cannot be rectified, products must be taken out of service and protected against unintentional operation.
2. Keep the door closed when not carrying out service and maintenance work.
CAUTION
Activities while the analyzer is in operation introduce risk of exposure to hazardous materials.
Follow standard procedures for limiting exposure to chemical or biological materials.
Follow workplace policies on personal protective equipment including wearing protective clothing, goggles and
gloves and limiting physical access to analyzer location.
Clean any spills following the appropriate site policies and cleaning procedures.
CAUTION
There is risk of injury from the analyzer door stop mechanism.
If the enclosure needs to be open, always open the analyzer door fully to ensure the analyzer door stop engages
properly.
2.5 Product safety
The product is designed to meet local safety requirements for the intended application, and has been tested
accordingly, leaving the factory in a condition in which it is safe to operate. All applicable regulations and
international standards have been observed. Devices connected to the analyzer must also comply with the applicable
safety standards, and users should follow the probe-specific product safety instructions.
2.6 IT security
Our warranty is valid only if the device is installed and used as described in the Operating Instructions. The device is
equipped with security mechanisms to protect it against any inadvertent changes to the settings.
IT security measures, which provide additional protection for the device and associated data transfer, must be
implemented by the operators themselves in line with their security standards.

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3Product description
3.1 The Raman Rxn2 analyzer
The Raman Rxn2 analyzer, powered by Kaiser Raman technology, is a for-purpose embedded system with built-in
Raman RunTime control software. Raman spectroscopy provides the chemical specificity of mid-infrared (IR)
spectroscopy and the sampling simplicity of near-IR spectroscopy. By operating in the visible or near-IR spectral
region, Raman spectroscopy allows vibrational spectra to be collected in situ, using fiber-coupled probes, without
sample purging, and without the use of specialty sampling devices.
There are four possible configurations of the Raman Rxn2 analyzer: single-channel, four-channel, hybrid, and
starter. All Raman Rxn2 analyzers employ a unique self-monitoring system to ensure the validity of each analysis.
The analyzer is capable of two-point self-calibration in extreme environments and utilizes self-diagnostics and
spectral correction methods when system calibration is unnecessary. The analyzer's precision is essential for robust
chemometric analyses and calibration transfer between instruments. The Raman Rxn2 suite of analyzers allows for
remote fiber-optic connections to probe sampling points for installation flexibility. And all configurations of the
Raman Rxn2 analyzer are designed for use with the Endress+Hauser line of Raman fiber-optic probes and optics. An
ergonomic mobile cart, including built-in probe and optic storage, is available as an option for all Raman Rxn2
configurations.
3.1.1 The Raman Rxn2 single-channel and four-channel configurations
The Raman Rxn2 single-channel configuration offers one fiber-optic sampling probe connector for the accurate
measurement, collection, monitoring, and analysis of a single sample. The Raman Rxn2 four-channel configuration
provides four fiber-optic sampling probe connectors. Raman Rxn2 single-channel or four-channel configurations are
available with a 532 nm, 785 nm, or 993 nm excitation wavelength laser.
Both the Raman Rxn2 single-channel and four-channel configurations are designed for use in an analytical or
process development laboratory for routine sample measurements, quality assurance, or process development
applications in life sciences, chemical, and food and beverage industries. The four-channel Raman Rxn2 was
designed for customers who need to support process development activities so that they can monitor multiple
vessels. The ability to follow several different reactions at the same time can quickly build process knowledge and
simplify technology transfer from a laboratory into a process environment.
With the Raman RunTime software embedded into the analyzer, the Raman Rxn2 single-channel and four-channel
analyzers meet the needs of both good laboratory practice (GLP) and good manufacturing practice (GMP) governed
areas within the pharmaceutical industry for process analytical technology (PAT) and quality by design (QbD)
applications.
3.1.2 The Raman Rxn2 hybrid configuration
The Raman Rxn2 hybrid configuration is unique because it contains connectors for an Rxn-20 large volumetric probe
and a second, alternate (ALT) backscattered probe. The Raman Rxn2 hybrid configuration is only available with a
785 nm excitation wavelength laser.
The two different probe types enable a variety of applications for solids, liquids, and turbid media. A backscattered
immersion probe is the preferred approach for measuring liquids because of its short focus, optical window, and
bubble-shedding design. The Rxn-20 probe is optimized for large volumetric measurements, enabling focus-free,
non-contact representative measurements of solids or turbid media. The hybrid configuration provides maximal
sampling flexibility for laboratory, quality control, and process development purposes.
With the Raman RunTime software embedded into the analyzer, the Raman Rxn2 hybrid configuration meets the
needs of both good laboratory practice (GLP) and good manufacturing practice (GMP) governed areas within the
pharmaceutical industry for process analytical technology (PAT) and quality by design (QbD) applications.

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3.1.3 The Raman Rxn2 starter configuration
The standard Raman Rxn2 starter configuration is the analyzer with a single Rxn-10 Raman spectroscopic probe.
There is an optional upgrade to configure the analyzer with up to four channels, and the analyzer is compatible with
the full range of Endress+Hauser liquid or bioprocessing probes. The Raman Rxn2 starter configuration is only
available with a 785 nm excitation wavelength.
The Raman Rxn2 starter configuration is designed for cart-portable or benchtop uses such as material quality,
reaction monitoring, basic science research, quality assurance, and unknowns identification. The variety of
non-contact or immersion optics compatible with the Rxn-10 probe provides sampling flexibility to support a variety
of applications.
3.2 Raman RunTime software overview
Raman RunTime embedded software is the control platform for the suite of Raman Rxn analyzers. Raman RunTime
software is intended for easy integration with standard multivariate analysis and automation platforms to enable a
real-time, in situ process monitoring and control solution. Raman RunTime presents an OPC and Modbus interface,
which provides clients with analyzer data as well as analyzer control functions. Raman RunTime is fully embedded
into Raman Rxn analyzers. Refer to the Raman RunTime Operating Instructions (BA02180C) for descriptions of
analyzer operations including analyzer operation, calibration, data models, and error reports.