Esco Labculture PLUS LP2-4S1 User manual

User
Manual
Esco Technologies, Inc.
2940 Turnpike Drive, Units 15-16 • Hatboro, PA 19040, USA
Toll-Free USA and Canada 1-877-479-3726
Tel [phone] • Fax [phone]
us.escoglobal.com • [e-mail]
Esco Micro Pte. Ltd.
21 Changi South Street 1 • Singapore 486 777
Tel [phone] • Fax [phone]
www.escoglobal.com • [email protected]m
Labculture•PLUS

Copyright Information
© Copyright 2011 Esco Micro Pte. Ltd. All rights reserved.
The information contained in this manual and the accompanying product is copyrighted and all rights are
reserved by Esco.
Esco reserves the right to make periodic minor design changes without obligation to notify any
person or entity of such change.
Labculture® and Sentinel™ are registered trademarks of Esco.
“Material in this manual is provided for informational purposes only. The contents and the product described in
this manual (including any appendix, addendum, attachment or inclusion), are subject to change without
notice. Esco makes no representations or warranties as to the accuracy of the information contained in this
manual. In no event shall Esco be held liable for any damages, direct or consequential, arising out of or related
to the use of this manual.”

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Class II Biological Safety Cabinet
INTRODUCTORY PAGES
i
Table of Contents
iii
Warranty Terms and Conditions
v
Introduction
v
1. Products Covered
v
2. Safety Warning
v
3. Limitation of Liability
v
4. European Union Directives on WEEE and RoHS
vii
Declaration of Conformity
USER SECTION
1
Chapter 1 - Product Information
1
1.1 Quick View
3
Chapter 2 - Sentinel Control System
3
2.1 Sentinel Control System
4
2.2 Menu Options
4
2.2.1 Settings
6
2.2.2 Calibration
6
2.2.3 Admin
7
2.2.4 Set Mode
7
2.3 Alarm and Warnings
9
Chapter 3 - Basic Cabinet Operation
9
3.1 Sash Window Operation
9
3.1.1 Sash Window State
9
3.1.2 Operating Motorized Sash Window
10
3.1.3 Using Sash Window
10
3.2 Starting and Shutting Down the BSC
10
3.2.1 Turning on the BSC
10
3.2.2 Turning off the BSC
10
3.3 Working in the BSC
11
3.4 Working Ergonomics
12
3.5 UV Lamps
12
3.6 Decontamination and Disinfecting Agents
15
Chapter 4 - Maintenance
15
4.1 Scheduled Maintenance
16
4.2 Maintenance/Service Log
APPENDIX

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iii
Class II Biological Safety Cabinet
Esco products come with either a 1, 2 or 3 year limited warranty, depending on the product purchased,
beginning on the date of shipment from any Esco international warehousing location. To determine which
warranty applies to your product, refer to the appendix below.
Esco's limited warranty covers defects in materials and workmanship. Esco's liability under this limited
warranty shall be, at our option, to repair or replace any defective parts of the equipment, provided if proven
to the satisfaction of Esco that these parts were defective at the time of being sold, and that all defective parts
shall be returned, properly identified with a Return Authorization.
This limited warranty covers parts only, and not transportation/insurance charges.
This limited warranty does not cover:
Freight or installation (inside delivery handling) damage. If your product was damaged in transit, you
must file a claim directly with the freight carrier.
Products with missing or defaced serial numbers.
Products for which Esco has not received payment.
Problems that result from:
oExternal causes such as accident, abuse, misuse, problems with electrical power, improper
operating environmental conditions.
oServicing not authorized by Esco.
oUsage that is not in accordance with product instructions.
oFailure to follow the product instructions.
oFailure to perform preventive maintenance.
oProblems caused by using accessories, parts, or components not supplied by Esco.
oDamage by fire, floods, or acts of God.
oCustomer modifications to the product
Consumables such as filters (HEPA, ULPA, carbon, pre-filters) and fluorescent / UV bulbs.
Esco is not liable for any damage incurred on the objects used on or stored in Esco equipment. If the
objects are highly valuable, user is advised to have in place independent external preventive
measures such as connection to a centralized alarm system.
Factory installed, customer specified equipment or accessories are warranted only to the extent guaranteed by
the original manufacturer. The customer agrees that in relation to these products purchased through Esco, our
limited warranty shall not apply and the original manufacturer's warranty shall be the sole warranty in respect
of these products. The customer shall utilize that warranty for the support of such products and in any event
not look to Esco for such warranty support.
Esco encourages all users to register their equipment online at www.escoglobal.com/warranty or complete the
warranty registration form included with each product.
ALL EXPRESS AND IMPLIED WARRANTIES FOR THE PRODUCT, INCLUDING BUT NOT LIMITED TO ANY IMPLIED
WARRANTIES AND CONDITIONS OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE
LIMITED IN TIME TO THE TERM OF THIS LIMITED WARRANTY. NO WARRANTIES, WHETHER EXPRESS OR
IMPLIED, WILL APPLY AFTER THE LIMITED WARRANTY PERIOD HAS EXPIRED. ESCO DOES NOT ACCEPT LIABILITY
BEYOND THE REMEDIES PROVIDED FOR IN THIS LIMITED WARRANTY OR FOR SPECIAL, INDIRECT,
CONSEQUENTIAL OR INCIDENTAL DAMAGES, INCLUDING, WITHOUT LIMITATION, ANY LIABILITY FOR THIRD-
PARTY CLAIMS AGAINST YOU FOR DAMAGES, FOR PRODUCTS NOT BEING AVAILABLE FOR USE, OR FOR LOST
WORK. ESCO'S LIABILITY WILL BE NO MORE THAN THE AMOUNT YOU PAID FOR THE PRODUCT THAT IS THE
SUBJECT OF A CLAIM. THIS IS THE MAXIMUM AMOUNT FOR WHICH ESCO IS RESPONSIBLE.

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Labculture•PLUS
These Terms and Conditions shall be governed by and construed in accordance with the laws of Singapore and
shall be subject to the exclusive jurisdiction of the courts of Singapore.
Technical Support, Warranty Service Contacts
USA: 1-877-479-3726
Singapore: [phone]
Global Email Helpdesk: supp[email protected]m
Visit http://www.escoglobal.com/ to talk to a Live Support Representative
Distributors are encouraged to visit the Distributor Intranet for self-help materials.
Product Appendix, Warranty Listings
Biological Safety Cabinets, Laminar Flow Cabinets,
HEPA-Filtered Cabinets (except Streamline brand)
The warranty periods for BSC may vary by country. Contact
your local distributor for specific warranty details.
Laboratory Fume Hoods
1 year limited.
Ductless Fume Hoods
3 years limited for Ascent Opti's, 5 years for Ascent Max's.
Cleanroom Equipment
1 year limited.
Laboratory Ovens and Incubators
1 year limited.
CO2Incubators
2 years limited.
Containment/Pharma Products
2 years limited.
Ultralow Temperature Freezer
3 years limited. 60 months on Compressor.
The warranty period starts two months from the date your equipment is shipped from Esco facility for
international distributors. This allows shipping time so the warranty will go into effect at approximately the
same time the equipment is delivered to the user. The warranty protection extends to any subsequent owner
during the warranty period. Distributors who stock Esco equipment are allowed an additional four months for
delivery and installation, providing the product is registered with Esco. User can register product online at
www.escoglobal.com/warranty or complete the warranty registration form included with each product.
Policy updated on 12th Apr 2011 (This limited warranty policy does not apply to products purchased before 12th
Apr 2011)

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Class II Biological Safety Cabinet
1. Products Covered
Esco Labculture Plus, Class II Biological Safety Cabinet
Electrical Rating
1.2 meters (4 feet)
1.8 meters (6 feet)
220-240 V AC, 50Hz, 1Φ
LP2-4S1
LP2-6S1
220-240 V AC, 60Hz, 1Φ
LP2-4S3
LP2-6S3
2. Safety Warning
Anyone working with, on or around this equipment should read this manual. Failure to read,
understand and follow the instructions given in this documentation may result in damage to the unit,
injury to operating personnel, and / or poor equipment performance.
Any internal adjustment, modification or maintenance to this equipment must be undertaken by
qualified service personnel.
The use of any hazardous materials in this equipment must be monitored by an industrial hygienist,
safety officer or some other suitably qualified individual.
Before you process, you should thoroughly understand the installation procedures and take note of
the environmental / electrical requirements.
In this manual, important safety related points will be marked with the symbol.
If the equipment is used in a manner not specified by this manual, the protection provided by this
equipment may be impaired.
3. Limitation of Liability
The disposal and / or emission of substances used in connection with this equipment may be governed by
various local regulations. Familiarization and compliance with any such regulations are the sole responsibility
of the users. Esco’s liability is limited with respect to user compliance with such regulations.
4. European Union Directive on WEEE and RoHS
The European Union has issued two directives:
• Directive 2002/96/EC on Waste Electrical and Electronic Equipment (WEEE)
This product is required to comply with the European Union’s Waste Electrical &
Electronic Equipment (WEEE) Directive 2002/96/EC. It is marked with the following
symbol:
Esco sells products through distributors throughout Europe. Contact your local Esco
distributor for recycling/disposal.
• Directive 2002/95/EC on Restriction on the use of Hazardous Substances (RoHS)
With respect to the directive on RoHS, please note that this hood falls under category 8 (medical devices) and
category 9 (monitoring and control instruments) and is therefore exempted from requirement to comply with
the provisions of this directive.

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Labculture•PLUS

vii
Class II Biological Safety Cabinet
Declaration of Conformation
In accordance to EN ISO/IEC 17050-1:2010
We, Esco Micro Pte. Ltd.
of 21 Changi South Street 1
Singapore, 486777
Tel: [phone]
Fax: [phone]
declare on our sole responsibility that the product:
Category : Class II Biological Safety Cabinet
Brand : Labculture Plus
Model : LP2-4S1, LP2-6S1
in accordance with the following directives:
2006/95/EEC : The Low Voltage Directive and its amending directives
92/31/EEC : The Electromagnetic Compatibility Directive and its amending
directives
has been designed to comply with the requirement of the following Harmonized Standard:
Low Voltage : EN 61010-1:2010
EMC : EN 61326-1:2006 Class B
Design/ : EN 12469 (2000) Class II Biological Safety Cabinet
Performance
Criteria
More information may be obtained from Esco’s authorized distributors located within the European Union. A
list of these parties and their contact information is available on request from Esco.
_______________________________
XQ Lin
Group CEO, Esco
This Declaration of Conformity is only applicable for 230V AC 50/60Hz units

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Labculture•PLUS

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Class II Biological Safety Cabinets
-
1.1 Quick View
1. Optional exhaust collar
2. Frameless shatter proof motorized sash
3. Raised armrest
4. Multi-piece work surface
5. Hinged front panel
6. Esco Sentinel microprocessor control system
7. Service fixtures
8. Universal electrical outlet
9. Optional support stand
1
2
3
4
5
6
7
8
9

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Class II Biological Safety Cabinets
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2.1 Sentinel Control System
1. Fan Button
oTurns on and turn off the fan.
2. Lamp Button
oTurns on and turn off the fluorescent lamps.
3. Socket Button
oTurns on and turn off the electrical socket (retrofit kit).
oThe maximum rating of all the outlets in the cabinet is 5 A. If overload, the fuse will blow.
4. UV Button
oTurns on and turn off the UV lamp.
oUV lamp can only be activated when the sash window is fully closed. Since the sash is
capable of filtering UV rays, users are protected from the harmful UV radiation.
5. Up (▲) and Down (▼) Arrow Button
oMove the menu options upwards and downwards.
oIncrease and decrease corresponding value inside one of the menu options.
oMove the sash window upward and downward
6. Set or Mute or Diagnostic Button
oProceed to the next step, level or sequence inside the menu options.
oTo mute the fully opened sash and airfail alarm sound
oEnter diagnostic mode
7. Menu Button
oTo enter and exit from the menu options.
oTo go back to the previous level of the menu options.
oTo access maintenance mode from error condition.
Menu
Button
Set/Mute/
Diag Button
Up
Button
Down
Button
LCD
Display
Fan
Button
Lamp
Button
Gas/Socket
Button
UV
Button
When you are entering menu options, the alarm will sound to indicate that the microprocessor is
not monitoring the operation of the cabinet. No further warnings will be given.
Visual
Alarm

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2.2 Menu Options
Please refer to the following diagram for complete reference to all menu options available.
MENU
SETTINGS
SET MODE
CALIBRATION
ADMIN
SET TIME
WARM UP
POSTPURGE
UV TIMER
VELOCITY UNIT
TEMP UNIT
SET CONSTANT
ZERO SENSOR
CALIB SENSOR
NORMAL
MAINTENANCE
NEW ADMIN PIN
NEW FAN PIN
A/F MONITOR
HH:MM
XX MINUTES
XX MINUTES
HH:MM
FPM | M/S
XXXX (Default 0009)
XXXX (Default 0000)
ENABLE | DISABLE
CELCIUS | FAHRENHEIT
RESET B/H/M
RESET UV/H/M
RESET DEFAULT
SASH SELF TEST
2.2.1 Settings
The user may use the settings menu function to customize the operation of the Cabinet to meet specific
application requirements.
2.2.1.1 Set Clock (Time)
Users can set the time by increasing/decreasing the hour and minute values. The correct time will be
maintained even after the unit is turned off.
MENU SETTINGS SET TIME HH:MM

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Class II Biological Safety Cabinets
2.2.1.2 Warm Up Timer
There will be a period of warm-up, before the fan is fully functioning. This is to ensure that the sensors, the
fan, and the control system are stabilized, as well as purging the work zone of contaminants. The default
setting is 3 minutes and user can set it up to 15 minutes.
MENU SETTINGS WARM UP XX MINUTES
2.2.1.3 Post Purge Timer
After the user switches off the fan, there will be a period of post-purge. This feature is to ensure that all
residual contaminants are purged from the work zone. The default setting is zero minute and user can set it
from zero minute to 15 minutes. Setting it to zero minute will disable this feature. However, it is
recommended to purge the Cabinet by leaving the fan on for around 3 minutes after the work is complete.
MENU SETTINGS POSTPURGE XX MINUTES
2.2.1.4 UV Timer
To switch off the UV lamp automatically after a fixed period, UV TIMER menu can be used. The UV TIMER can
be set up to 18 hours. By default, this feature is disabled. UV lamp, then, has to be switched off manually. Esco
does not recommend that you leave the UV lamp on for more than 60 minutes or overnight as it shortens the
lifespan of the UV lamp.
MENU SETTINGS UV TIMER HH:MM
2.2.1.5 UV Timer
In this unit, the system has a one push sash up movement control which means that if the sash is below the
nominal height and the up button is pushed once, the sash will reach the nominal height and stop. Otherwise
it will reach the top most position and stop.
To make that possible, the microprocessor has to store the exact location of the sash, so for that it has to
initially move the sash to the fully open position and store that position to be the reference position. This
feature is called sash self test.
This feature is automatically activated only in three conditions:
•The first time the microprocessor is installed in the cabinet during production.
•The BSC functionality mode is set to Maintenance Mode and after maintenance brought back to the
normal mode.
•The BSC functionality mode is set to Cleaning Mode and after cleaning brought back to the normal
mode.
MENU SETTINGS SASH SELF TEST YES | NO
2.2.1.6 Airflow Unit Selection
Using this option, you can select the unit in which air velocity is measured and displayed. You can choose
between meter per second (m/s) and feet per minute (fpm). When the fan is running and also during the
calibration process, airflow measurement is displayed in the selected unit.
MENU SETTINGS VELOCITY UNIT M/S
FPM
2.2.1.7 Temperature Unit Selection
Using this option, you can select the unit in which temperature is measured and displayed. You can choose
between Celsius and Fahrenheit. The exhaust temperature is displayed in the selected unit.
MENU SETTINGS TEMP UNIT FAHRENHEIT
CELSIUS

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2.2.2 Calibration
The purpose of calibration is to ensure the accuracy of the airflow display and alarm (if present). This involves
measuring airflow with reference instrumentation and establishing reference between airflow sensor(s) on the
Cabinet to the standard reference. Calibration should only be carried out by trained personnel.
SET CONSTANT
MENU CALIBRATION ZERO SENSOR
CALIB SENSOR
2.2.2.1 Set Constant
Every sensor manufactured by Esco has a specific Sensor Constant which is used for temperature
compensation performed by the temperature sensor.
2.2.2.2 Zero Sensor
This option is to let the controller record the specific sensor output voltage and correspond it to 0 m/s or 0
fpm.
2.2.2.3 Calib Sensor
This option allows proper calibration and operation of the airflow sensor alarm. There will be three points to
be calibrated, namely inflow fail point, inflow nominal point, and downflow nominal point.
2.2.3 Admin
The admin menu allows you to change both Fan and Admin. PIN, also to disable it (not recommended). The
reset blower hour meter and reset UV hour meter functions are usually used after you change the filter and
UV lamp. While the reset default function will return the options in the settings menu to their factory settings.
2.2.3.1 New Admin. PIN
Admin. PIN restricts access to MENU functions, including service functions, like calibration. User must enter
four digits PIN before accessing MENU. Admin. PIN has higher priority and can be used to control the fan
(override Fan PIN).
Setting PIN to 0000 will disable this feature.
MENU ADMIN NEW ADMIN PIN XXXX
2.2.3.2 New Fan PIN
Fan PIN restricts access to fan control. User must enter four-digit PIN before switching fan on or off. As such, it
can restrict access to operating the Cabinet by unauthorized personnel. It will also prevent unauthorized
shutdown of the Cabinet when continuous operation is required. Note that continuous operation is
recommended for better safety. Fan PIN is also needed to disable the alarm when the sash is fully raised and
cleaning needs to be performed.
Setting the PIN to 0000 will disable this feature.
MENU ADMIN NEW FAN PIN XXXX
2.2.3.3 A/F Monitor
Whenever the air velocity falls below the fail point, the air fail alarm will be triggered. This option is used to
enable/ disable alarm.
MENU ADMIN A/F MONITOR ENABLE
DISABLE

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Class II Biological Safety Cabinets
2.2.3.4 Reset Blower Hour Meter
This option is used to reset the blower hour meter. The blower hour meter indicates how long the blower has
been in operation. The value can also provide some help in setting up maintenance schedule, including filter
change.
MENU ADMIN RESET B/H/M
2.2.3.5 Reset UV Hour Meter
This option is used to reset the UV hour meter. The UV hour meter indicates how long the UV lamp has been
used. Maximum counter is set at 9999 hours. The counter value can be checked while in maintenance mode.
Please reset the UV hour meter after each UV replacement.
MENU ADMIN RESET UV/H/M
2.2.3.6 Reset Default
User can reset the default setting by choosing this option. The features being reset are warm-up period (3
minutes), post-purge period (0 minute), UV timer (0 minute), airflow unit (Metric), temperature unit (Celsius),
Admin. PIN (0009), and Fan PIN (0001).
Note that the calibration settings cannot be reset as it may cause the Cabinet to operate in an unsafe manner.
The hour meters cannot be reset either.
MENU ADMIN RESET DEFAULT
2.2.4 Set Mode
Cabinet has two working mode, the default normal mode which is used in a day to day activity, and
maintenance mode.
MENU SET MODE
NORMAL
MAINTENANCE
2.2.4.1 Normal Mode
Every time the Cabinet is restarted, this mode will be activated by default. In this mode, all alarms and
interlocks are enabled.
2.2.4.2 Maintenance Mode
Maintenance mode should only be accessed by qualified personnel during maintenance. In this mode, all
alarms are disabled and all interlocks are defeated.
2.3 Alarms and Warnings
BSC uses alarms to indicate that the condition inside the BSC is not safe for the operator, so check the LCD
display to understand the cause of these alarms. The most common alarm is the SASH ALARM that indicates
that the sash is neither at the normal operating height nor at fully closed position (UV mode) –this condition
can easily be corrected by putting the sash at the appropriate operation position.
Another warning that should be acted upon is AIR FAIL! which indicates that there is airflow failure. The
operator should check if there is any obstruction to the airflow, and correct it if possible. However, if the
problem continues, the operator should stop working as the BSC’s protection may have been compromised.
Call service or Esco’s local distributor.
Other alarms that indicate a failure or an error in the BSC system:
•ERR.AIRFAIL will be displayed if the blower is turned off while there is an airflow failure.
•ERR.MSWITCH will be displayed if the microprocessor (controller) detects more than one microswitch
activated at the same time, which is impossible, as the sash can only be at one position at one time.
This indicates a failure in the sash detection system.
•ERR.CALIB will be displayed if the airflow velocity sensor is not yet calibrated.

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Class II Biological Safety Cabinets
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3.1 Sash Window Operation
3.1.1 Sash Window State
3.1.2 Operating Motorized Sash Window
The motorized sash uses a “push and hold” mechanism, so if you remove your finger from the button the sash
will stop immediately –this is a safety feature to control the closure and prevent anything getting trapped in
the aperture as the sash descends.
Lower Sash from Fully Open Position
When the sash is fully open, pressing the down button and holding it will cause the sash to move to the Safe
Height setting and stop. If the fluorescent lights are on as the sash descends, they will stay on as long as the
sash stops in the Safe Position (not applicable for NC2). If you release the button before the sash has reached
Safe Position the lights will switch off automatically.
Lower Sash from Safe Height Position
When the sash is at safe operating height pressing the down button and holding it will cause the sash to move
down to the fully closed position and stop. If the fluorescent lights are on as the sash descends, they will
switch off automatically as soon as the sash reaches fully closed. If you release the button before the sash has
reached the fully closed position the lights will switch off automatically
Raise Sash from Fully Closed Position
When the sash is fully closed, pressing the up button and holding it will prompt the user to input the password
to turn on the fan. If the password is correct, if it was on fan will turn on and the sash will move up to the Safe
Height setting and stop.
Raise Sash from Safe Height Position
When the sash is safe operation position, pressing the up button and holding it will cause the sash to move up
to the fully open position and stop. If the fluorescent lights are on as the sash rises, they will stay on as long as
the sash is allowed to fully open. Stopping the sash midway will cause the lights to switch off automatically.
Sash is fully open
Blower can be active
Fluorescent lights can be used
Unsafe working condition
Sash is in safe position
Blower can be activated
Fluorescent lights can be used
Safe working condition
Sash is fully closed
Blower can’t be activated
Fluorescent lights unusable

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3.1.3 Using Sash Window
The sash window should be fully closed when the cabinet is not in use. This helps keep the work zone
interior clean.
The sash window should always be in the normal operating height at all times when the cabinet is in
use. Even if the cabinet is left unattended, but the blower is on, the sash window should never be
moved from the normal operating height, unless during loading or unloading of materials/apparatus
into the cabinet.
The alarm will be activated whenever the sash window is moved from the normal operating height.
Whenever the sash window is moved to the correct height from a higher or lower position, the light
will automatically be turned on as a signal to the user.
The sash window may be opened to its maximum position for the purpose of loading/unloading of
materials/apparatus into the cabinet. When the sash window is fully opened, the alarm sound may be
muted by pressing MUTE button but, will be automatically sounded again after 5 minutes to remind
the user that it is not safe to work in the cabinet and the light will be turned on to facilitate cleaning.
3.2 Starting and Shutting Down the BSC
3.2.1 Turning on the BSC
1. Raise the sash to the indicated normal operational height (READY state). The lamp will turned on
when this height is reached.
2. Turn on the fan by pressing the FAN button. Input the Fan PIN if asked (if PIN ≠0000). This will start
the warm up procedure (default: 3 minutes). All buttons are diabled during warm up period.
3. The BSC is ready for work.
3.2.2 Turning off the BSC
1. Turn off the fan by pressing the FAN button. Input the Fan PIN if asked (if PIN ≠0000). This will start
the post purge procedure (default: 0 minute). All buttons are diabled during post purge period.
2. Lower the sash to the fully closed position (the display will show UV MODE). The sash can be lowered
immediately after turning off the fan as it will not interrupt the post purge procedure.
3. Turn on the UV lamp (when present) to decontaminate the work area by pressing the UV button.
Leave the UV lamp on to make sure the decontamination is done effectively . The UV lamp can
only be turned on after the post purge procedure is fini shed.
3.3 Working in the BSC
Surface-decontaminate the work area (work surface, back and side wall, UV lamp, electrical outlet,
service fixture and the inner surface of the sash window) before and after using the BSC.
Allow the BSC to purge any contaminant by allowing the blower to operate at least 3 minutes before
and after using the BSC.
Surface-decontaminate any item (including your arms) or apparatus with appropriate disinfectant
before entering or exiting the work area.
Place the waste container (containment bag, pippete discard pans, etc.) inside the BSC work area.
Place all items and apparatus in the safe working area.
Safe Working Area for Multi Tray BSC