Esco Labculture Troubleshooting guide

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Esco Controlled Environment
Laboratory and Cleanroom
Equipment Solutions
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User and Service Manual
Class ll, Type A2
Biological Safety Cabinets

User
And
Service
Manual
Thank you for purchasing this Esco Biological Safety
Cabinet. Please read this manual thoroughly to
familiarize yourself with the many unique features
and exciting innovations we have built into your new
equipment. Esco provides many other resources at our
website, www.escoglobal.com, to complement this
manual and help you enjoy many years of productive
and safe use of your Esco products.
US $ 50.00
Europe €40.00
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Esco LA2 User & Service Manual (Version 4)
Released 28 July 2008
For Technical Service, contact
North America
Esco Technologies, Inc.
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Rest of World
Esco Micro Pte. Ltd.
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Biological Safety Cabinets

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COPYRIGHT INFORMATION
© Copyright 2008 Esco Micro Pte. Ltd. All rights reserved.
The information contained in this manual and the accompanying product is copyrighted and all rights are reserved
by Esco.
Esco reserves the right to make periodic minor design changes without obligation to notify any
person or entity of such change.
Sentinel™ and Labculture®are registered trademarks of Esco.
“Material in this manual is provided for informational purposes only. The contents and the product described in this manual
(including any appendix, addendum, attachment or inclusion), are subject to change without notice. Esco makes no representations
or warranties as to the accuracy of the information contained in this manual. In no event shall Esco be held liable for any damages,
direct or consequential, arising out of or related to the use of this manual.”

i
:
Table of Contents
Warranty Terms and Conditions .................................................................................................... v
Introduction ................................................................................................................................... vii
1. Products Covered................................................................................................................................................. vii
2. Safety Warning .................................................................................................................................................... vii
3. Limitation Of Liability ........................................................................................................................................ viii
4. European Union Directive on WEEE and RoHS................................................................................................ viii
5. Symbols.................................................................................................................................................................. ix
Declaration Of Conformity............................................................................................................ xi
About Esco Biological Safety Cabinets ......................................................................................xiii
User Section
1. Basic Product Information ....................................................................................................... 1
1.1Quickview....................................................................................................................................................1
1.2Airflowpattern............................................................................................................................................2
2. Installation ................................................................................................................................ 3
2.1 Pre-requisites ......................................................................................................................................... 3
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5IFGPMMPXJOHSFRVJSFNFOUTTIPVMECFUBLFOJOUPBDDPVOU 
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4VQQPSU3FRVJSFNFOUT 
&YIBVTU3FRVJSFNFOUT 
&MFDUSJDBM3FRVJSFNFOUT 
4FSWJDF-JOF3FRVJSFNFOUT 
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2.2ExhaustCollarInstallation..........................................................................................................................8
2.3DrainValveInstallation.............................................................................................................................11
2.4CabinetPlumbing......................................................................................................................................12
2.5ConnectingTheCabinet............................................................................................................................14
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2.6 Performance Validation / Certification ....................................................................................................15
%JTDMBJNFS 
3. Operating Your Cabinet. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ..............................................................17
3.1Sentinel
TM Control System Overview ........................................................................................................17
3.2ConceptofDiferentSashWindowStates................................................................................................19
3.3 Starting The Cabinet .................................................................................................................................19
3.4WorkingInTheCabinet............................................................................................................................20
3.5WorkingErgonomics.................................................................................................................................22
Tabl e o f C onte n t s

ii :
$MBTT**5ZQF"#JPMPHJDBM4BGFUZ$BCJOFUtUser and Service Manual
3.6CleaningAndShuttingDownTheCabinet.............................................................................................23
3.7MenuOptions............................................................................................................................................25
4FUUJOHT$MPDL5JNFSBOE6OJUT 
4FU$MPDL5JNF 
8BSN6Q5JNFS 
1PTU1VSHF5JNFS 
675JNFS 
&915JNFS 
"JS7FMPDJUZ6OJU4FMFDUJPO 
5FNQFSBUVSF6OJU4FMFDUJPO 29
$BMJCSBUJPO 29
"ENJOJTUSBUPS"DDFTTBOE1JO 
/FX"ENJO1*/ 
/FX'BO1*/ 
"'.POJUPS 
3FTFU#MPXFS)PVS.FUFS 
3FTFU67)PVS.FUFS 
3FTFU%FGBVMU 
4FUUJOHUIF.PEF 
/PSNBM.PEF 
.BJOUFOBODF.PEF 
4. Maintaining Your Cabinet ..................................................................................................... 35
Log Record........................................................................................................................................................36
Service Section
1. Re-Certification And Maintenance By Service Personnel................................................... 39
1.1CertificationFlowchart..............................................................................................................................40
1.2AirflowAdjustment...................................................................................................................................40
1.3DamperAdjustment..................................................................................................................................41
1.4UnitRe-certification..................................................................................................................................42
1.5CalibrationofSentinel™Control.............................................................................................................49
4FUUJOH$BCJOFUUP.BJOUFOBODF.PEF49
4FOTPS$POTUBOU5FNQFSBUVSF4FOTPS"DUJWBUJPO 
8BSNJOH6QUIF4QFFE$POUSPM 
$BMJCSBUJOHUIF*OnPX'BJM4FU1PJOU 
$BMJCSBUJOHUIF*OnPX/PNJOBM4FU1PJOU
$BMJCSBUJOHUIF%PXOnPX/PNJOBM4FU1PJOUEPXOnPXWFMPDJUZ 
4FUUJOH$BCJOFUCBDLUP/PSNBM.PEFBOE'JOBM$IFDLJOH 
1.6ReplacementofFilters...............................................................................................................................52
1.7 Replacement of Blower.............................................................................................................................53
1.8ReplacementofAirflowSensor................................................................................................................55
1.9ReplacementofSashGlass........................................................................................................................56
1.10ReplacementofFlourescentLampandUVLamp.................................................................................57
Tabl e o f C onte n t s

iii
:
1.11DecontaminationProcedure...................................................................................................................57
8BSOJOHPO'PSNBMJO&YQPTVSF 
$PNNPO-JTUPG&RVJQNFOU6TFE 
"QQSPQSJBUF1SPUFDUJWF$MPUIJOH 
$PNNPO"QQBSBUVT 
$PNNPO1SFQBSBUPSZ4UFQT 
%FDPOUBNJOBUJPO1SPDFTT 
'PSNBMEFIZEF"NNPOJB$PNCJOBUJPO 
1BSBGPSNBMEFIZEF"NNPOJVN#JDBSCPOBUF$PNCJOBUJPO 
$PNQMFUJPO4UFQT 
7BMJEBUJPOPG%FDPOUBNJOBUJPO1SPDFEVSF0QUJPOBM 
2. Troubleshooting...................................................................................................................... 65
2.1HardwareandSoftwareTroubleshooting...............................................................................................65
)BSEXBSF5SPVCMFTIPPUJOH 
4PGUXBSF5SPVCMFTIPPUJOH 
2.2OperationModeSummary.......................................................................................................................93
3. Product Specification ............................................................................................................. 95
3.1LA2EngineeringDetails...........................................................................................................................95
3.2LA2(A-Series)-230VCabinetsTechnicalSpecification Summary ........................................................97
3.3LA2(A-Series)-115VCabinetsTechnicalSpecification Summary ........................................................98
3.4LA2(L-Series)TechnicalSpecificationSummary......................................................................................99
3.5ElectricalSchematicDrawingFor220-240V,AC,50/60 Hz Variant (LA2-_A1/3) ................................101
3.6ElectricalSchematicDrawingFor220-240V,AC,50/60 Hz Variant (LA2-_L1/3)..................................103
3.7ElectricalSchematicDrawingFor220-240V,AC,50/60 Hz Variant (LA2-_A1/3-M) ............................105
3.8ElectricalSchematicDrawingFor220-240V,AC,50/60 Hz Variant (LA2-_L1/3-M) .............................107
3.9ElectricalSchematicDrawingFor110-130V,AC,60 Hz Variant (LA2-_A2) .........................................109
3.10EnvironmentalandElectricalRequirements.......................................................................................111
Replacement Parts List (Standard) ............................................................................................ 113
Replacement Parts List (Motorized Window)........................................................................... 117
Appendix
A. Further Information and Reference Materials ...................................................................123
B. Log Record ............................................................................................................................ 125
Tabl e o f C onte n t s

$MBTT**5ZQF"#JPMPHJDBM4BGFUZ$BCJOFUtUser and Service Manual

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:
WARRANTY TERMS AND CONDITIONS
Warranty Terms and Conditions
Esco warrants that this equipment will perform according to specifications for 3 years from the date of purchase.
Esco’s limited warranty covers defects in materials and workmanship. Esco’s liability under this limited warranty shall
be, at our option, to repair or replace any defective parts of the equipment, provided if proven to the satisfaction of
Esco that these parts were defective at the time of being sold, and that all defective parts shall be returned, properly
identified with a Return Authorization.
This limited warranty covers parts only, and not transportation/ insurance charges.
This limited warranty does not cover:
√Freight or installation (inside delivery handling) damage. If your product was damaged in transit, you must file a
claim directly with the freight carrier.
√Products with missing or defaced serial numbers
√Products for which Esco has not received payment
√Problems that result from:
¤ External causes such as accident, abuse, misuse, problems with electrical power, improper operating environmen-
tal conditions
¤ Servicing not authorized by Esco
¤ Usage that is not in accordance with product instructions
¤ Failure to follow the product instructions
¤ Failure to perform preventive maintenance
¤ Problems caused by using accessories, parts, or components not supplied by Esco
¤ Damage by fire, floods, or acts of God
¤ Customer modifications to the product
√Consumables such as filters (HEPA, ULPA, carbon, pre-filters) and fluorescent / UV bulbs
Factory installed, customer specified equipment or accessories are warranted only to the extent guaranteed by the
original manufacturer. The customer agrees that in relation to these products purchased through Esco, our limited
warranty shall not apply and the original manufacturer’s warranty shall be the sole warranty in respect of these
products. The customer shall utilise that warranty for the support of such products and in any event not look to Esco
for such warranty support.
Esco encourages all users to register their equipment online at www.escoglobal.com/warranty or complete the war-
ranty registration form included with each product.
ALL EXPRESS AND IMPLIED WARRANTIES FOR THE PRODUCT, INCLUDING BUT NOT LIMITED TO ANY IMPLIED WAR-
RANTIES AND CONDITIONS OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE LIMITED IN TIME
TO THE TERM OF THIS LIMITED WARRANTY. NO WARRANTIES, WHETHER EXPRESS OR IMPLIED, WILL APPLY AFTER
THE LIMITED WARRANTY PERIOD HAS EXPIRED. ESCO DOES NOT ACCEPT LIABILITY BEYOND THE REMEDIES PRO-
VIDED FOR IN THIS LIMITED WARRANTY OR FOR SPECIAL, INDIRECT, CONSEQUENTIAL OR INCIDENTAL DAMAGES,
INCLUDING, WITHOUT LIMITATION, ANY LIABILITY FOR THIRD-PARTY CLAIMS AGAINST YOU FOR DAMAGES, FOR
PRODUCTS NOT BEING AVAILABLE FOR USE, OR FOR LOST WORK. ESCO’S LIABILITY WILL BE NO MORE THAN THE
AMOUNT YOU PAID FOR THE PRODUCT THAT IS THE SUBJECT OF A CLAIM. THIS IS THE MAXIMUM AMOUNT FOR
WHICH ESCO IS RESPONSIBLE.

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$MBTT**5ZQF"#JPMPHJDBM4BGFUZ$BCJOFUtUser and Service Manual
These Terms and Conditions shall be governed by and construed in accordance with the laws of Singapore and shall
be subject to the exclusive jurisdiction of the courts of Singapore.
Technical Support, Warranty Service Contacts
Toll-Free USA and Canada 888-375-ESCO
Singapore: [phone]
Global Email Helpdesk: [email protected]
Visit http://www.escoglobal.com/ to talk to a Live Support Representative
Distributors are encouraged to visit the Distributor Intranet for self-help materials.
Policy updated on 30th Jan 2007 (This limited warranty policy does not apply to products purchased before 30th Jan 2007).
Warranty Terms and Conditions

vii
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2. SAFETY WARNING
t Anyone working with, on or around this equipment should read this manual. Failure to read, understand and
follow the instructions given in this documentation may result in damage to the unit, injury to operating personnel,
and / or poor equipment performance.
t"OZJOUFSOBMBEKVTUNFOUNPEJmDBUJPOPSNBJOUFOBODFUPUIJTFRVJQNFOUNVTUCFVOEFSUBLFOCZRVBMJmFETFSWJDF
personnel.
t 5IFVTFPGBOZIB[BSEPVTNBUFSJBMJOUIFDBCJOFUNVTUCFNPOJUPSFECZBOJOEVTUSJBMIZHJFOJTUTBGFUZPGmDFSPS
some other suitably qualified individual.
t &YQMPTJWFPSJOnBNNBCMFTVCTUBODFTTIPVMEOFWFSCFVTFEJOUIFDBCJOFUVOMFTTBRVBMJmFETBGFUZQSPGFTTJPOBMIBT
evaluated the risk involved.
t *GDIFNJDBMSBEJPMPHJDBMPSPUIFSOPONJDSPCJPMPHJDBMIB[BSETBSFCFJOHVTFEJOUIFDBCJOFUBEEJUJPOBMQSPUFDUJWF
measures should be taken. Besides that, the operation should be monitored by a suitably trained individual.
t 5IFCJPMPHJDBMTZNCPMTIPXOBMPOHTJEFPOUIFGSPOUQBOFMPGUIFDBCJOFUJOEJDBUFTUIFQSFTFODFPG
biological substances that pose a threat to human health.
t 5IJTDBCJOFUJTTVJUBCMFGPSBHFOUTDMBTTJmFEVOEFSCJPMPHJDBMTBGFUZMFWFMTBOE
t 'PSVTFPGCJPMPHJDBMTBGFUZMFWFMBHFOUTJOUIFDBCJOFUUIFBHFOUTTIPVMECFQMBDFEJOBOFHBUJWFQSFTTVSFBSFB
Please note that while doing so, the operator should be wearing a full body positive pressure isolation suit.
1. PRODUCTS COVERED
This manual is applicable and specific to the following Esco products.
Note:
1. The A - Series (LA2-_A1) (220-240V, AC, 50Hz, 1Ø) are designed to comply with the European EN 12469 requirements.
2. The L - Series (LA2-_L1) (220-240V, AC, 50Hz, 1Ø) are designed to comply with the European EN 12469 requirements.
Introduction
Note
:
Electrical Nominal Size
0.9 meters (3’) 1.2 meters (4’) 1.5 meters (5’) 1.8 meters (6’)
7"$)[ -"" -"" -"" -""
7"$)[ -"". -"". -"". -"".
7"$)[ -"" -"" -"" -""
7"$)[ -"". -"". -"". -"".
7"$)[ -"" -"" -"" -""
7"$)[ -"". -"". -"". -"".
7"$)[ -"- -"- -"- -"-
7"$)[ -"-. -"-. -"-. -"-.
7"$)[ -"- -"- -"- -"-
7"$)[ -"-. -"-. -"-. -"-.
INTRODUCTION

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$MBTT**5ZQF"#JPMPHJDBM4BGFUZ$BCJOFUtUser and Service Manual
Introduction
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safely changed.
NB: Cytotoxic substances cannot be inactivated by conventional formaldehyde decontamination
t #FGPSFZPVQSPDFFEZPVTIPVMEUIPSPVHIMZVOEFSTUBOEUIFJOTUBMMBUJPOQSPDFEVSFTBOEUBLFOPUFPGUIF
environmental/electrical requirements of the cabinet
t *OUIJTNBOVBMJNQPSUBOUTBGFUZSFMBUFEQPJOUTXJMMCFNBSLFEXJUIUIJTTZNCPM
t *GUIFFRVJQNFOUJTVTFEJOBNBOOFSOPUTQFDJmFECZUIJTNBOVBMUIFQSPUFDUJPOQSPWJEFECZUIJTFRVJQNFOUNBZ
be impaired.
3. LIMITATION OF LIABILITY
The disposal and / or emission of substances used in connection with this cabinet may be governed by various local
regulations. Familiarization and compliance with any such regulation are the sole responsibility of the users of the
cabinet. Esco’s liability is limited with respect to user compliance with such regulations.
4. EUROPEAN UNION DIRECTIVE ON WEEE AND RoHS
The European Union has issued two directives:
t %JSFDUJWF&$PO8BTUF&MFDUSJDBMBOE&MFDUSPOJD&RVJQNFOU8&&&
The objective of the WEEE directive is to promote “… the reuse, recycling and other forms of
recovery of such wastes (WEEE) so as to reduce the disposal of waste besides improving the
environmental performance of all operators involved in the life cycle of electrical and electronic
equipment, e.g. producers, distributors and consumers…” and hence this directive refers to the
disposal of this cabinet within the EU. A “wheelie bin” sticker (shown alongside) has to be paste on
all products covered by this directive, indicating that at the time of disposing of the product, it should
not be grouped together with general unsorted municipal waste. Instead, distributors of electrical and electronic
equipment should be responsible for the collection and scrapping of the products they have sold. Please note that
this cabinet has been classified as “fixed industrial equipment” and hence the WEEE directive is not applicable to its
disposal. You may, at the time of disposing this cabinet, still contact your local Esco distributor who can arrange for
collection and recycling of this cabinet for a reasonable fee.
t %JSFDUJWF&$PO3FTUSJDUJPOPOUIFVTFPG)B[BSEPVT4VCTUBODFT3P)4
With respect to the directive on RoHS, please note that this cabinet falls under category 9 (monitoring and control
instruments) and is therefore exempted from requirement to comply with the provisions of this directive.

ix
:
5. SYMBOLS
Information in this manual may be prefaced with the following symbols. They are provided to help you identify
important operational, safety, maintenance or conformance issues.
&MFDUSJDBM)B[BSE%BOHFSPGFMFDUSJDTIPDL
5VSO 0GG "OE %JTDPOOFDU 'SPN .BJO 4VQQMZ #FGPSF 1SPDFFEJOH %P OPU QFSGPSN UIJT PQFSBUJPO XIJMF UIF VOJU JT
PQFSBUJPOBM
The Biohazard SymbolPOUIFGSPOUQBOFMPGUIFDBCJOFUJOEJDBUFTUIFQSFTFODFPGCJPMPHJDBMTVCTUBODFTUIBUQPTFBUISFBU
UPIVNBOIFBMUI
Important Information:3FBEBOEVOEFSTUBOECFGPSFQSPDFFEJOH*NQPSUBOU TBGFUZ SFMBUFEQPJOUTXJMMCF NBSLFE XJUI
UIJTTZNCPM
Approved Service Engineer Only:0QFSBUJPOUPCFQFSGPSNFEPOMZCZBQQSPWFEFOHJOFFS
Introduction

$MBTT**5ZQF"#JPMPHJDBM4BGFUZ$BCJOFUtUser and Service Manual

xi
:
Declaration of Conformity *
In accordance with EN 45014:1998
We, Esco Micro Pte Ltd
based at 21 Changi South Street 1
Singapore 486777
Tel: [phone]
Fax: [phone]
declare on our sole responsibility that the product:
Equipment : Biological Safety Cabinet
Model : Labculture Class II (LA2-3A_,LA2-4A_,LA2-5A_,LA2-6A_)
(LA2-3A_-M ,LA2-4A_-M ,LA2-5A_-M ,LA2-6A_-M)
(LA2-3L_, LA2-4L_,LA2-5L_,LA2-6L_)
(LA2-3L_-M , LA2-4L_-M ,LA2-5L_-M ,LA2-6L_-M )
in accordance with the following directives:
  t&&$ 5IF-PX7PMUBHF%JSFDUJWFBOEJUTBNFOEJOHEJSFDUJWFT
  t&&$ 5IF&MFDUSPNBHOFUJD$PNQBUJCJMJUZ%JSFDUJWF
and its amending directives
has been designed to comply with the requirements of the following Harmonized Standard:
  t-PX7PMUBHF &/
  t&.$ &/$MBTT#
  t%FTJHO &/$MBTT**#JPMPHJDBM4BGFUZ$BCJOFU
Performance
Criteria
More information may be obtained from Esco’s authorized distributors located within the EU. A list of these parties
and their necessary contact information is available on request from Esco.
Lim Lay Yew
Chief Executive Officer
* Applicable only to 220-240V, AC, 50 Hz cabinets
%FDMBSBUJPOPG$POGPSNJUZ

$MBTT**5ZQF"#JPMPHJDBM4BGFUZ$BCJOFUtUser and Service Manual

xiii
:
About Esco Biological Safety Cabinets
ABOUT ESCO BIOLOGICAL SAFETY CABINETS
5IFEJGGFSFOUOPNFODMBUVSFVTFECZWBSJPVTTUBOEBSETBOEUIFJOnPXSFRVJSFNFOU
Standard origin The standard nomenclature Minimum inflow requirement (m/s)
NSF / ANSI 49 64"$MBTT**5ZQF" 
NSF / ANSI 49 64"$MBTT**5ZQF" 
+*4, +BQBO$MBTT**5ZQF"# 5ZQF"5ZQF#
&/ &VSPQFBO6OJPO$MBTT** 
"4 "VTUSBMJB$MBTT** 5PQBTTBJSCBSSJFSUFTU
#JPTBGFUZ-FWFM#4-
Lethality Medium Cure Example
1Safe -JRVJE Yes #BDJMMVT4VCUJMJT
24PNF -JRVJE 4PNF )*7
34FSJPVT "JSCPSOF 4PNF TBC
4&YUSFNF "JSCPSOF None &CPMB
Name / Number Governing Body Referenced
NSF / ANSI 49 5IF/BUJPOBM4BOJUBUJPO'PVOEBUJPO/4'*OUFSOBUJPOBMIUUQXXXOTGPSH 64(MPCBM
/PUFT
4UBOEBSEIBTCFFOXJEFMZBEPQUFEOPUPOMZJOUIF6OJUFE4UBUFTCVUBMTPBSPVOEUIFXPSME
"QQMJFTUP$PWFST
Class II biological safety cabinets
Name / Number Governing Body Referenced
&/ *OUFSOBUJPOBM0SHBOJ[BUJPOGPS4UBOEBSEJ[BUJPOIUUQXXXJTPPSH &6(MPCBM
/PUFT
5IF&VSPQFBO/PSNJTUIFMBUFTUJOUFSOBUJPOBMTUBOEBSEEFWFMPQFEBTBEJSFDUDPOTFRVFODF
PGUIF&VSPQFBO6OJPOTIBSNPOJ[BUJPOFGGPSUT*USFQMBDFTQSFWJPVTTUBOEBSETTVDIBTUIF(FSNBO
TUBOEBSE%*/UIF#SJUJTITUBOEBSE#4BOEUIF'SFODITUBOEBSE/'95IF&/
IBTCFFOBEPQUFEPGmDJBMMZBTBOBUJPOBMTUBOEBSEJOBMMNFNCFSOBUJPOTPGUIF&6
"QQMJFTUP$PWFST
Class I biological safety cabinets
Class II biological safety cabinets
Class III biological safety cabinets
Name / Number Governing Body Referenced
"4 4UBOEBSET"VTUSBMJBIUUQXXXTUBOEBSETDPNBV "VTUSBMJB/FX;FBMBOE
/PUFT
5IF"4JTUIF"VTUSBMJBOTUBOEBSEGPSCJPMPHJDBMTBGFUZDBCJOFUSZ*UIBTBMTPMBSHFMZCFFO
BEPQUFEBTBOBUJPOBMTUBOEBSEJOOFBSCZ/FX;FBMBOE*UBQQMJFTUPCPUI$MBTT*BOE$MBTT**DBCJ
OFUSZXJUIBGPDVTPODBCJOFUDPOTUSVDUJPOEFTJHOBOEQFSGPSNBODF"VTUSBMJBOTUBOEBSEDBCJOFUT
JODPSQPSBUFUXJOCMPXFSTBOEBSFMBSHFMZEJGGFSFOUGSPN&VSPQFBOBOE"NFSJDBOiUZQFwNPEFMT
"QQMJFTUP$PWFST
Class I biological safety cabinets
Class II biological safety cabinets
Biological safety cabinet plays a significant role in many pharmaceutical, clinical, microbiological and industrial
laboratories. Its basic function is to protect the operator and the environment from biological hazards that would
otherwise pose a threat to human life during microbiological work.
At Esco, we encourage customers to learn more about the functions and operating principles of the biological safety
cabinets available in the market today. We believe this will help you choose the cabinet that is most suitable for your
specific needs.
MAJOR INTERNATIONAL STANDARDS
International standards play an important role in harmonization and ensuring that cabinets meet established industry
guidelines for safety and performance. Consequently, you should understand the scope and application of these
standards. The following tables detail some common international standards:

xiv :
$MBTT**5ZQF"#JPMPHJDBM4BGFUZ$BCJOFUtUser and Service Manual
About Esco Biological Safety Cabinets
BIOLOGICAL SAFETY CABINET SUMMARY TABLE OF FEATURES
FURTHER INFORMATION
For further information on the topics discussed above, we have many documents available at www.escoglobal.com.
Here you will find the most up-to-date information in far more detail than is possible to include in this manual.
B
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A
B
IN
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F
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Name / Number Governing Body Referenced
+*4, +BQBOFTF*OEVTUSJBM4UBOEBSET$PNNJUUFFIUUQXXXKJTDHPKQFOH Japan
/PUFT
5IF+*4,XBTEFSJWFEGSPNUIF/4'BOEVTFEPOMZJO+BQBO+VTUMJLF/4'+*4,
POMZBQQMJFTUP$MBTT**CJPMPHJDBMTBGFUZDBCJOFUT
"QQMJFTUP$PWFST
Class II biological safety cabinets
F
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R
INFOR
M
ATION
Class
5ZQF
Biosafety
Level
1SPUFDUJPO
Offered Min. Inflow
Velocity
Recirc.
Air
Exhaust
Air
Contaminated
1MFOVN4VSSPVOEFE
By
Exhaust Alternatives
Oper-
ator
1SPE
uct
I
 ¸˚ NTGQN 0%  0VUTJEFBJS-BCSPPN *OTJEFSPPN)BSE%VDU
t #BTJDEFTJHOBOEGFBUVSFTFU
t 6OmMUFSFE SPPN BJS nPXT JOUP UIF DBCJOFU QBTTFT UISPVHI UIF XPSL[POF BOE UIFO UISPVHI B mMUSBUJPO TZTUFN USBQQJOH BJSCPSOF
DPOUBNJOBOUT$MFBOEFDPOUBNJOBUFEBJSJTFYIBVTUFEGSPNUIFDBCJOFU
t 5IFTDPQFBOEBQQMJDBUJPOPG$MBTT*DBCJOFUTJTMJNJUFEBOEJUJTMBSHFMZDPOTJEFSFEPCTPMFUF
II A1
 ¸¸ NTGQN   0VUTJEFBJS-BCSPPN *OTJEFSPPN5IJNCMF%VDU
t 3PPNBJSFOUFSTUIFDBCJOFUUISPVHIBGSPOUJOMFUHSJMMFBOEJTmMUFSFECFGPSFFOUFSJOHUIFXPSL[POFDPOUBNJOBUFEBJSJTQBTTFTUISPVHIBmMUSBUJPOTZTUFN
USBQQJOHBJSCPSOFDPOUBNJOBOUT$MFBOEFDPOUBNJOBUFEBJSJTSFDJSDVMBUFEUISPVHIUIFXPSL[POFPSFYIBVTUFEGSPNUIFDBCJOFU
t 5ZQF"DBCJOFUTIBWFBQPTJUJWFMZQSFTTVSJ[FEQMFOVNCPSEFSJOHUIFBNCJFOUFOWJSPONFOU
t 5ZQF"JTOPXMBSHFMZDPOTJEFSFEPCTPMFUF
II A2
 ¸¸ NTGQN   /FHBUJWFQSFTTVSF *OTJEFSPPN5IJNCMF%VDU
t /FHBUJWFQSFTTVSFTVSSPVOETUIFQPTJUJWFMZQSFTTVSJ[FEDPOUBNJOBUFEQMFOVNQSPWJEJOHBEEFEQSPUFDUJPOJOUIFDBTFPGBMFBLJOUIF
QPTJUJWFMZQSFTTVSJ[FEQMFOVN
t .PTU&VSPQFBO+BQBOFTFBOE"VTUSBMJBO$MBTT**DBCJOFUTIBWFBJSnPXSFDJSDVMBUJPOBOEFYIBVTUSBUJPTTJNJMBSUP$MBTT**5ZQF"DBCJOFU
BMPOHXJUIUIFOFHBUJWFQSFTTVSFJTPMBUFEQMFOVNPQFSBUJOHJOUIFTBNFQSJODJQMFBTUIF/4'$MBTT**5ZQF"CVUXJUIMPXFSJOnPX
WFMPDJUZSFRVJSFNFOU
II B1
 ¸¸ NTGQN   /FHBUJWFQSFTTVSF )BSEEVDUPOMZ
t 5ZQF#DBCJOFUTBMTPIBWFBEFEJDBUFEFYIBVTUGFBUVSFGPSXPSLEPOFJOTJEFUIFDBCJOFUUPXBSETUIFCBDL
t 5PYJDDIFNJDBMTTIPVMEPOMZCFVTFEJGUIFZEPOPUJOUFSGFSFXJUIXPSLXIFOSFDJSDVMBUFEJOUIFEPXOnPX
t 5ZQF#DBCJOFUTIBWFMBSHFMZCFFOSFQMBDFECZ5ZQF#UIFZBSFOPXHFOFSBMMZDPOTJEFSFEPCTPMFUF
II B2
 ¸¸ NTGQN 0%  /FHBUJWFQSFTTVSF )BSEEVDUPOMZ
t 5ZQF#DBCJOFUTFYIBVTUBMMJOnPXBOEEPXOnPXBJSBGUFS)&1"mMUSBUJPOUPUIFFYUFSOBMFOWJSPONFOU
t 5ZQF#DBCJOFUTBSFTVJUBCMFGPSXPSLXJUIUPYJDDIFNJDBMTVTFEBTBOBEKVODUUPNJDSPCJPMPHJDBMQSPDFTTFT
t 5ZQF#DBCJOFUTNBZCFDPOTJEFSFEUPCFUIFTBGFTUPGBMM$MBTT**CJPMPHJDBMTBGFUZDBCJOFUTJOUIFTFOTFUIBUUIFUPUBMFYIBVTUGFBUVSF
BDUTBTBGBJMTBGFBHBJOTUEPXOnPXBOEPSFYIBVTU)&1"mMUSBUJPONBMGVODUJPO)PXFWFSUIFDBCJOFUSFRVJSFTBTUBCMFCVJMEJOHFYIBVTU
TZTUFNUIBUJTQSFDJTFMZUVOFEUPUIFDBCJOFUBJSnPXSFRVJSFNFOUT
III
 ¸¸ 11B
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)BSE%VDU
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t 8PSLJTQFSGPSNFEUISPVHIHMPWFQPSUTJOUIFGSPOUPGUIFDBCJOFU
t %VSJOHSPVUJOFPQFSBUJPOOFHBUJWFQSFTTVSFSFMBUJWFUPUIFBNCJFOUFOWJSPONFOUJTNBJOUBJOFEXJUIJOUIFDBCJOFU
t $MBTT***DBCJOFUTQSPWJEFTQSPEVDUQSPUFDUJPOBOEQSFWFOUDSPTTDPOUBNJOBUJPOPGTBNQMFT
t &YIBVTUBJSJTVTVBMMZ)&1"mMUFSFEBOEJODJOFSBUFE"MUFSOBUJWFMZEPVCMF)&1"mMUSBUJPOXJUIUXPmMUFSTJOTFSJFTNBZCFVUJMJ[FE
t $MBTT***DBCJOFUTVTVBMMZFYIBVTUBJSCBDLUPUIFMBCPSBUPSZBJSNBZBMTPCFFYIBVTUFEUPUIFFYUFSOBMFOWJSPONFOU
t 8IFO B EFEJDBUFE EVDUXPSL TZTUFN JT FNQMPZFE UIFZ BSF TVJUBCMF GPS XPSLJOH XJUI UPYJD DIFNJDBMT BT BO BEKVODU UP NJDSPCJPMPHJDBM
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USER SECTION

$MBTT**5ZQF"#JPMPHJDBM4BGFUZ$BCJOFUtUser and Service Manual