Fein Optic IRB20 User manual

Fein
IRB20 Inverted Microscope User’s Manual
info@feinoptic.com

1Fein
IRB20 Microscope Components
Eyepieces Condenser
Lamp Housing
Nosepiece
Mechanical
Stage
Rheostat
On/O
Power
Siedentopf
Trinocular Head
C-Mount Adapter Phase
Slider
Beam Splitter
Coarse Focus
Fine Focus
Stage
X-Y Control

2
Fein
IRB20 Microscope Components
Mechanical
Stage
On/O
Power
Stage
X-Y Control
1. Eyepiece
2. Siedentopf Trinocular Head
3. Phase Contrast Slider
4. Aperture Diaphragm Lever
5. Filter Slider
6. Lamp Housing
7. Condenser
8. Round Insert on Stage
9. Fixed Stage
10. Carrying Handle
11. Objective Lens
12. Nosepiece
13. Power Socket
14. Fuse Box
15. Power Switch
16. Surplus Water Plate
17. Mechanical Stage X-Y Knob
18. Light Rheostat Control
19. Coarse & Fine Focus Control
20. Attachable Mechanical Stage
21. Beam Splitter Knob

IRB20 Inverted Microscope Assembly
3Fein
• Once the microscope is assembled, turn on the main power source.
• Place the sample on the stage.
• Move the 10x objective lens into the light path.
• Focus on the sample using the coarse focus and then the ne focus.
• Adjust the brightness using the rheostat control.
• Adjust the interpupillary distance and the eyepiece diopter.
• Adjust the aperture diaphragm.
• Once the sample is in focus, lters may be placed in the optical path.

IRB20 Inverted Microscope Operation Instructions
4
Fein
Do not shake or drop the microscope.
Do not expose the microscope to direct sun, high temperatures, high humidity, dust,
or damp environments. Use a at work surface.
When moving the microscope use
both hands, holding the handle at the
back (1) and the frame at the front (2)
as shown in the image at right.
Set the power switch to o “O” before
replacing a bulb or fuse, and wait until the
lamp is cool. The power switch is located
on the side of the microscope. Microscope
uses 5W LED bulb. (Part # M40-001).
Voltage range of 100~240V (47-63Hz) is supported.
Turn the rheostat control knob (1) to the
minimum and turn the power switch (2) to
the on “I”position. Rotate the rheostat knob
to increase or decrease the brightness of the
illumination.
Adjust the aperture diaphragm lever (1) to
obtain the best image contrast.

IRB20 Inverted Microscope Operation Instructions
5Fein
Place the lter (1) inside
the lter holder (2), and
insert into the lter slot on
the condenser.
The tension of the coarse focusing knob can be
adjusted by holding the tension adjustment ring (1)
and rotating it. Turning the adjustment ring clockwise
will increase the focus tension and turning it counter-
clockwise will decrease the tension.
If the stage is drifting (falling) out of focus, tighten the
focus tension ring (clockwise) to create more tension
and keep the stage from drifting.
Do NOT rotate the coarse & ne focus knobs in
opposite directions at the same time!
Adjust the eyetubes to set the interpupillary
distance for your eyes. If multiple people
use the microscope daily, note the number
of your interpupillary setting for ease of
future setup.
Set both eyepieces to“0” scale on the
diopter. Look through the microscope
with only one eye and focus. Now
close the eye you just looked through
and look through the other eyepiece
with the other eye only. If the sample is
out of focus, adjust the diopter on that
eyepiece until it is clearly in focus.

IRB20 Inverted Microscope Operation Instructions
6
Fein
The eyepieces can be locked in place with
a set screw (1). To change the eyepiece,
loosen the set screw and pull the eyepiece
out of the eyetube.
FIXED STAGE:
Insert the round plate (1) into the xed
stage.
Install the stage clips (2) into the slot
on the xed stage.
MECHANICAL STAGE:
Insert the round plate (1) into the stage.
Install attached mechanical stage (2) with screws (3) provided.
Place petri dish or stage inserts (4) into stage as needed.

IRB20 Inverted Microscope Bulb Replacement
7Fein
Turn o and unplug the microscope prior to
changing the bulb.
1. Remove the screw with the allen wrench.
2. Lift the illumination cover.
3. The bulb is located at the front of the housing.
Once the cover is removed, unscrew the two
screws in the image shown below that lie on
either side of the wires leading to the bulb.

IRB20 Inverted Microscope Bulb & Fuse Replacement
8
Fein
When you lift the bulb housing be careful
since the wires are still connected. In order
to replace the bulb remove the solder joints
from the wires connected to the bulb.
Unscrew the 2 screws holding the bulb to
the housing. Screw the new bulb into the
housing. Re-solder the wires back onto the
bulb in the same positions they were in
prior to bulb replacement.
Replacement Bulb: M40-001 (5W LED).
FUSE REPLACEMENT:
Turn o and unplug the microscope prior to fuse replacement.
The fuse box is installed below the power socket.
Fuse: 5x20mm, 2A, 250V.

IRB20 Inverted Microscope Phase Contrast Setup
9Fein
Ensure phase slider is right side up and slide into the condenser.
Place phase objective in the light path and put the phase slider in the position that
matches the phase objective magnication.
Remove the eyepiece and insert the phase centering telescope. Use the phase
centering screws to align the bright and dark rings as shown in the illustrations
below. Image at left shows what the rings might look like prior to alignment. Image
at right shows once they are aligned.

IRB20 Inverted Microscope Trouble Shooting
10
Fein
TROUBLE POSSIBLE CAUSE SOLUTION
Field of view
obstructed or
irregular illumination
Nosepiece is not clicked into position. Move nosepiece into position.
Filter slider is not in place. Adjust lter slider.
Phase Contrast slider is not in place. Adjust phase slider.
Dust in eld of view
Objective lens is dirty. Clean objective lens.
Sample has dirt or dust on it. Use a new sample.
Eyepiece is dirty. Clean eyepiece lens.
Hot spot on image Aperture diaphragm isn’t open enough. Adjust aperture diaphragm.
Poor image: low
contrast, image is not
sharp, image details
are not clear.
Objective is not in correct position. Rotate nosepiece into position.
Objective lens is dirty. Clean objective lens.
Culture dish is too thick. Use normal size culture dish.
Sample is dirty. Clean sample.
Part of image is not
clear.
Objective is not in correct position. Rotate nosepiece into position.
Sample is not placed in correct place on stage. Adjust sample and holder.
Phase contrast
observation is poor.
Annular spot did not focus with dish holder. Readjust phase alignment.
Culture dish bottom is not at. Use standard culture dish.
Coarse focus knob is
too tight. Tension is too tight. Loosen tension adjustment ring.
Nosepiece slides
down automatically. Tension is too loose. Tighten tension adjustment ring.
Binocular eld of view
is inconsistent.
The interpupillary distance is not set properly. Adjust interpupillary distance.
Diopter is not set properly. Adjust diopter.
Left eye and right eye have dierent eyepieces
in them.
Make sure a matching pair of
eyepieces are being used.
When using the high
power objective it is
touching the sample.
The culture dish bottom is too thick. Use a standard culture dish.
The bulb does not
work.
No bulb is in place. Install a bulb.
Bulb or fuse is broken. Replace bulb or fuse.
The power cord is not plugged in or turned on. Ensure cord is plugged in and
unit is turned on.
Bulb burns out
quickly. Improper bulb is being used. Use M40-001 5W LED bulb only.
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