Fein Optic RB20 User manual

Fein
RB20 Biological Microscope
User’s Manual
[e-mail]

Fein
Microscope Components
Eyepieces
Condenser
Objective Lens
1
Coarse Adjustment
Fine Adjustment
Tension
Adjustment
Microscope Frame /
Carrying Handle
Condenser Adjustment
Knob

Nosepiece
Mechanical
Stage
Fein
Microscope Components
2
Lock Screw
Fixed Koehler
Illumination
Focus Arm
Trinocular Head
Vertical
Adjustment
Knob
Horizontal
Adjustment
Knob
Power Switch
Light
Adjustment
Knob

Before Use
3Fein
Do not shake or drop the microscope.
Do not expose the microscope to direct sun, high temperatures, high humidity, dust,
or damp environments. Use a at work surface.
When moving the microscope use
both hands, holding the handle at the
back (1) and the frame at the front (2)
as shown in the image at right.
Set the power switch to o “O” before
replacing a bulb or fuse, and wait until the
lamp is cool. The power switch is located
on the side of the microscope.
Voltage range of 100~240V (47-63Hz) is supported.
Turn the rheostat control knob (1) to the
minimum and turn the power switch to the on
“I” position. Rotate the rheostat knob to increase
or decrease the brightness of the illumination. 1
1
2

4
Fein
Microscope Assembly
Follow the numbers below when assembling the microscope.
Eyepiece
Condenser
Objectives
Power Cord
1
2
3
4

Microscope Assembly
5Fein
Assemble the Condenser:
Rotate the coarse focus knob (1) to raise the
stage to its highest position.
Fully loosen the condenser lock-screw (2).
Insert the condenser into the hole of base
according to the arrowhead pointed (3), until
the condenser is aligned with the base, and
rotate the condenser to make the handle face
forward. Tighten the condenser lock-screw (2)
of condenser, then raise the condenser with the
up-down knob to the highest position.
2
Assemble the Objective:
Rotate the coarse focusing knob (1) to lower
the state to the lowest position. Then install
the objectives into the nosepiece (2) from
the lowest magnication to the highest in a
clockwise direction.
1
3
1
1
2

Fein 6
Microscope Assembly
Assemble the Eyepiece:
Take o the eyepiece tube cover (1).
Insert the eyepiece (2) into the eyepiece tube,
until the eyepiece is ush with the eyepiece
tube.
Tighten the eyepiece with M2.5 inner hexagon
lock-screw (3).
1
3
2
Assemble the Eyepiece:
Take o the eyepiece tube cover (1).
Insert the eyepiece (2) into the eyepiece tube,
until the eyepiece is ush with the eyepiece
tube.
Tighten the eyepiece with M2.5 inner hexagon
lock-screw (3).
1
3
2

Microscope Operation
7Fein
Turning the Lamp ON:
Set the power switch to the ON position “-”
(1). Adjust the illumination knob rotating
clockwise to increase the light intensity and
rotating counterclockwise to decrease the
light intensity.
Placing the Specimen Slide:
Push the spring lever (1) of the specimen
holder backwards. Place the specimen
slide (2) into the clip, then slowly move the
specimen holder lever back into place. This
will secure the specimen slide. Rotate the X
and Y axis knobs (3) on the stage until the
center of the specimen slide is aligned with
the objective.
Adjust the Focus:
Rotate the condenser adjustment knob (1)
raising it to the highest position. Move the
4x objective into the optical path. Looking
through the eyepieces, rotate the coarse
focusing knob (2) until the specimen appears
in the eld of view and begins to come into
focus. Rotate the ne focusing knob (3) to
bring the specimen into crisp focus.
1
2
12
3
1
2
3

Fein 8
Microscope Operation
Adjust the Focusing Tension:
If it becomes hard to move the coarse focus
knob, or the stage drifts and the sample falls
out of focus the tension needs to be adjusted.
Tighten the tension by adjusting the tension
adjustment ring (1) in the direction of the
arrow shown at right. Loosen the tension by
rotating the tension adjustment ring in the
opposite direction.
Adjusting the Interpupillary Distance:
When observing with both eyes, adjust
the eyepieces (2) to t your personal
interpupillary distance. The scale between
the eyepieces (1) notes the interpupillary
distance setting. It’s a good idea to note
this number when working in a setting
where multiple people are using the same
instrument. This allows you to easily reset
your interpupillary distance.
1
Aperture Diaphragm:
The aperture diaphragm dictates the
numerical aperture (NA) of the illumination.
The best resolution, contrast, and depth
of eld is obtained when the NA of the
illumination matches with the NA of the
objective. Rotate the diaphragm adjustment
ring (1) so the pointer matches up with the
inscription of the objective being used.
1
1
2
1
2

9Fein
Microscope Operation
Using the 100x Immersion Oil Objective:
Start with the 4x objective and focus on
the sample. Place a drop of immersion oil
(1) on the specimen. Rotate the nosepiece
counterclockwise so the 100x objective is in the
light path and the oil seals the space between
the objective and the cover slip. Use the ne
focus knob to obtain a clear image. Make sure
there are no air bubbles in the oil. If there are,
rotate the nosepiece slightly to remove them.
After use, wipe the lens with a tissue moistened
with microscope cleaning solution.
Using Filters:
Filters can increase the contrast in microscopy
images. The lter sits on top of the light as
shown at the right. The RB20 accepts a 34mm
diameter lter.
C-Mount assembly:
Loosen the trinocular head set screw (1)
and remove the trinocular port dust cap
(2). Remove any dust cover caps from the
c-mount (3). Insert the c-mount into the
trinocular port as image shows at right and
tighten the set screw (1). Connect the camera
to the c-mount threads (4) and re-tighten the
set screw. When the eyepieces are in focus,
adjust the focus on the c-mount to parfocal
the camera with the eyepieces.
1
1
3
24

Fein 10
Microscope Operation
Using the Storage Box:
When the microscope is not in use, the power
cord can be placed in the storage box. Press
the position (1) of the storage box cover with
thumb. When you hear a click, push up and
put the power cord into the storage box.
Close the back cover by pressing down on
the cover. 1

Troubleshooting
11 Fein
TROUBLE POSSIBLE CAUSE SOLUTION
Field of view is dark
even when light is on
The eld diaphragm is not large enough. Enlarge the eld diaphragm
Condenser is too low. Adjust the position of condenser.
The edge of the eld
of view is dark or not
even.
The nosepiece is not in the right position. Turn the nosepiece into the right
position.
Stain or dust has accumulated on the lens such
as condenser, objective, or eyepiece. Clean the lens.
Stain or dust is
observed in the eld
of view.
Stains have accumulated on the specimen. Clean the specimen.
Stains have accumulated on the lens. Clean the lens.
Glare or hot spot on
observed image.
Condenser is set too low. Raise condenser.
Aperture iris diaphragm is closed too much. Open aperture iris diaphragm.
Unclear image
There’s no cover glass on the specimen. Add the cover glass.
The cover glass is not standard. Use a standard cover glass with
thickness of 0.17mm.
The slide is upside down. Adjust the slide.
The immersion oil has accumulated on the dry
objective.
Clean the objective thoroughly.
The immersion oil is not being used for oil
objective.
Use immersion oil.
Air bubble is in the immersion. Get rid of the air bubbles.
The aperture diaphragm is not opened
correctly.
Adjust the aperture diaphragm.
Stain or dust has accumulated on the eyepiece
lens.
Clean the lens.
The condenser is too low. Adjust the condenser.
One side of the eld
of view is dark or the
image moves while in
focus.
The specimen is not xed. Fix the specimen.
The nosepiece is not in the right position. Turn the nosepiece into the right
position.
Condenser centered incorrectly. Center the condenser.
The eyes feel tired
easily. The right
eld of view doesn’t
superpose with the
left.
Interpupillary distance is wrong. Adjust the interpupillary
distance.
Eyepieces for the left eye and right eye are
dierent. Use the same eyepiece.

IRB20 Inverted Microscope Bulb & Fuse Replacement
12
Fein
TROUBLE POSSIBLE CAUSE SOLUTION
Coarse focusing knob
is too tight. Tension knob is too loose. Tighten it a little.
Stage declines itself,
cannot stay on the
focal plane when
observing.
Tension knob is too loose. Tighten it a little.
Coarse focusing knob
can’t rise. The limit stop knob is locked. Loosen the knob.
Coarse focusing knob
can’t decline. The base of the condenser is too low. Raise the base.
Cannot move the
slide smoothly.
The slide is not xed correctly. Adjust it
The movable specimen holder is not xed
properly. Adjust it
The image moves
obviously when
touching the stage.
The stage is fastened incorrectly. Fasten the stage correctly.
The LED light does
not work.
No power supply. Check the connection of the
power cable.
The LED bulb is not installed correctly. Install it correctly.
The LED bulb is burnt out. Replace it.
The bulb burns out
frequently. The wrong bulb is being used. Replace it with the correct bulb.
The illumination is
not bright enough.
The wrong bulb is being used. Replace it with the correct bulb.
The illumination adjustment is not set in a
bright position.
Adjust the illumination knob to
intensify the light.
Troubleshooting

Bulb Replacement:
13 Fein
Before performing any of these steps the
microscope MUST be turned o and unplugged.
With the microscope powered o and
unplugged, gently lay the microscope down on a
sturdy surface ensuring the carrying handle side
is down.
Locate the 4 rubber feet on the underside
of the microscope. Remove the rubber feet
exposing the 4 screws securing the bottom
plate of the microscope.
Gently lay microscope on
carrying handle.
Using an Allen wrench remove the 4 screws
securing the bottom plate of the microscope.
With all 4 screws removed, gently remove the
bottom plate exposing the internal electrical
components of the microscope.

The electrical components are mounted onto
the internal side of the bottom plate.
IRB20 Inverted Microscope Bulb & Fuse Replacement
14
Fein
Bulb Replacement:
With all 4 screws removed, carefully remove
the bottom plate exposing the internal
components of the microscope.
Locate the 2 screws securing the LED bulb
in place. Using a screw driver, remove the 2
screws.
Locate the black wire connection. Using your
thumb and forenger, pinch the sides of the
black wire plug connection and gently pull it
from the wire housing.

Bulb Replacement:
15 Fein
Place the new LED bulb (part# RB20-001) on to
the LED bulb platform and attach using the small
set screws that were previously removed.
Insert the black plastic wire plug back into
the wire housing.
With the LED bulb replaced, position the
bottom of the microscope plate back onto
the microscope frame. Using a screw driver,
secure the bottom plate with the 4 set screws.
Place the 4 rubber feet over the top of the 4
set screws.
LED replacement bulb part #RB20-001
Return the microscope to its upright position.
Plug the microscope back in, power on, and
adjust the rheostat control as needed.
[e-mail]
www.feinoptic.com
Fein