GeneReach POCKIT User manual

1 2017/03
USER MANUAL
Manufacturer:
GeneReach Biotechnology Corporation
TEL: [phone] / FAX: [phone]
No. 19, Keyuan 2nd Road, Central Taiwan Science Park, Taichung City 407, Taiwan
E-mail: [e-mail] / Website: www.genereach.com

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CONTENT
Symbols.......................................................................................................................................... 1
Section 1. Introduction................................................................................................................... 2
1.1 Intended Use......................................................................................................................... 2
Section 2. General Information...................................................................................................... 3
2.1 Product Components............................................................................................................ 3
2.2 Specifications....................................................................................................................... 3
2.3 Testing Capacity................................................................................................................... 3
2.4 Equipment and Materials Required But Not Provided......................................................... 3
2.5 Front View............................................................................................................................ 4
2.6 Rear View............................................................................................................................. 5
2.7 Software Version.................................................................................................................. 5
Section 3. Important Notes............................................................................................................. 6
3.1 Safety Information................................................................................................................ 6
Section 4. Operation....................................................................................................................... 7
4.1 Switch On............................................................................................................................. 7
4.2 Choose Detection Wavelength ............................................................................................. 7
4.3 Set up Date and Time........................................................................................................... 9
4.4 Run iiPCR Reaction........................................................................................................... 10
4.5 Display the Result .............................................................................................................. 12
4.6 Review the Raw Data......................................................................................................... 13
4.7 Exit the Experiment............................................................................................................ 13
4.8 Errors Occurred During the Operation............................................................................... 14
4.9 Switch Off .......................................................................................................................... 15
Section 5. Cleaning and Maintenance.......................................................................................... 16
5.1 General Care....................................................................................................................... 16
5.2 Dealing with Spills............................................................................................................. 16
Troubleshooting............................................................................................................................ 17
Reference...................................................................................................................................... 18

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Symbols
ConformitéEuropéenne
EU authorized representative
Supplementary fuse
Grounded conductor
Date of manufacturing
Manufacturer
Catalogue number
Serial number
Alternative current
Caution, consult instruction for use
WEEE symbol
This product is manufactured in compliance with the European
Union’s Waste Electrical & Electronic Equipment (WEEE)
Directive 2012/19/EU. Please follow your institutional
requirements for disposal of the accessories.

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Section 1. Introduction
The POCKIT™Nucleic Acid Analyzer (POCKIT) is designed for performing insulated
isothermal polymerase chain reaction (iiPCR) (Chang et al., 2012; Tsai et al., 2012). It provides
iiPCR-based analysis and is equipped with two optical channels (520 nm, 550 nm) for multiplex
detection. The qualitative test results are readily displayed on the LCD monitor, and
automatically saved in a SD card. It generates results for up to 8 nucleic acid samples within 1
hour.
1.1 Intended Use
POCKIT is intended to provide qualitative detection of nucleic acid targets using fluorescence-
based iiPCR reagents. Intended users are properly-trained laboratory technicians familiar with
molecular biology techniques, and those who want to perform iiPCR or its relative reagent kits
produced by GeneReach.
This instrument is intended for research purpose.

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Section 2. General Information
2.1 Product Components
POCKIT™Nucleic Acid Analyzer × 1 unit
User Manual × 1 copy
Warranty Card × 1 copy
Holder × 1 piece
2.2 Specifications
Dimensions: 280 mm (W) × 250 mm (D) × 85 mm (H)
Net Weight: 2.1 kg
Power Supply: 100-120/200-240 VAC, 50/60 Hz, 2A
Fuses: 2A, 250VAC, 5 × 20 mm fast-acting, low breaking capacity glass tube fuses.
Operating Temperature: 15 ~ 35°C
2.3 Testing Capacity
Number of Samples: 1 ~ 8 reactions per run
Reaction Volume: 45 ~ 55 l per reaction
2.4 Equipments and Materials Required But Not Provided
-Nucleic acid extraction kit
-Nucleic acid amplification reagents and kits
-200 l and 1000 l micropipette, tips, and 1.5 ml tubes
-R-tubes and Caps
-cubee™mini-centrifuge
-Disposable gloves
*Note: To avoid power failure, connecting POCKIT to an uninterrupted power system (UPS)
and/or voltage regulator is highly recommended.

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2.5 Front View
No
Item
Description
1
Lid
Isolates the heat source, and prevents light interference on the
reaction chamber.
2
Reaction well
Accommodate the R-tube holder to perform the amplification.
3
Cooling vents
Releases the heat generated during reaction.
4
Control panel
A touchscreen where users key in the operation commands
and view the results.
5
SD card slot
A slot where users insert the SD card to run POCKIT and
save reaction raw data.
6
Holder
Load R-tubes into the reaction wells.
②
③
④
⑥
①
⑤

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2.6 Rear View
No.
Item
Description
1
Main switch
To turn on/off the main power
2
Socket
To connect to the power cord
2.7 Software Version
No.
Item
Description
1
Software Version
Current software version on POCKIT system.
②
①
①

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Section 3. Important Notes
Check the device upon receiving. If any items are missing or harmed, please contact your local
distributor or GeneReach immediately for replacement. Do not use any damage items as they
may lead to poor performance of the test or harm the user.
3.1 Safety Information
DO NOT OPEN LID
DURING REACTION
Please carefully read these warnings, precautions and safety/operating instructions
completely before operating this product.
Electromagnetic Compatibility: This product complies with the emissions and immunity
requirements of IEC 61326. It is advisable to evaluate the electromagnetic environment prior
to operating the device. Do not use this device in close proximity to sources of strong
electromagnetic radiation (for example, unshielded intentional radio frequency (RF) sources).
Strong electromagnetic radiation may interfere with the proper operation of the device.
Transport condition at -29 ~ 50°C, 55 ± 20% R.H.
Storage condition at 10 ~ 40°C, 55 ± 20% R.H.
Operation altitude: under 5000 m.
Do not touch any plug and electric switch with wet hands.
This product should be placed in a dry, clean and ventilated indoor place. Strictly keep away
from moisture.
Switch off and unplug the device before moving it.
Hold the plug while pulling the power cord out from the electrical outlet. Do not pull it by
the cord directly.
Do not place the power cord on any hot surfaces.
Pack the device carefully before moving and delivering. Vibration or crash may cause damage.
Users should revalidate the device after it has been moved.
Do not look directly into the LED light in the reaction wells.
Only use consumables and accessories provided from GeneReach.
Make sure the ambient temperature during reaction is kept at 15 ~ 35°C.
SD card should not be removed until the device is switched off.
Do not disassemble the device if any problem occurs. Contact your local distributor or
GeneReach directly for troubleshooting.
In case of emergency or abnormal conditions, turn off the power switch or unplug. Do not
block access to power switch or plug.
When working around the device, always wear a lab coat, disposable gloves and protective
goggles.
Waste materials, such as gloves and R-tube, may lead to contamination of other experiments.
Please discard them carefully according to your local regulation.

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Section 4. Operation
4.1 Switch On
Plug in the POCKIT and switch on the main power located in the back. The instrument will
perform a self-test.
*Note: If self-testing runs longer than 15 minutes, please see Troubleshooting.
Caution: Make sure the power plug is connected to a grounded receptacle to prevent
electrical shock or fire.
4.2 Choose Detection Wavelength
1) After self-testing, choose the detection wavelength according to the kits or methods used and
press “OK”.

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2) “System Ready” will be shown after choosing the detection wavelength.
3) Before the reaction starts, to change the detection wavelength, press “CH Select” and
choose again.