GeneReach POCKIT User manual
i
CONTENT
Symbols.......................................................................................................................................... 1
Section 1. Introduction................................................................................................................... 2
1.1 Intended Use......................................................................................................................... 2
Section 2. General Information...................................................................................................... 3
2.1 Product Components............................................................................................................ 3
2.2 Specifications....................................................................................................................... 3
2.3 Testing Capacity................................................................................................................... 3
2.4 Equipment and Materials Required But Not Provided......................................................... 3
2.5 Front View............................................................................................................................ 4
2.6 Rear View............................................................................................................................. 5
2.7 Software Version.................................................................................................................. 5
Section 3. Important Notes............................................................................................................. 6
3.1 Safety Information................................................................................................................ 6
Section 4. Operation....................................................................................................................... 7
4.1 Switch On............................................................................................................................. 7
4.2 Choose Detection Wavelength ............................................................................................. 7
4.3 Set up Date and Time........................................................................................................... 9
4.4 Run iiPCR Reaction........................................................................................................... 10
4.5 Display the Result .............................................................................................................. 12
4.6 Review the Raw Data......................................................................................................... 13
4.7 Exit the Experiment............................................................................................................ 13
4.8 Errors Occurred During the Operation............................................................................... 14
4.9 Switch Off .......................................................................................................................... 15
Section 5. Cleaning and Maintenance.......................................................................................... 16
5.1 General Care....................................................................................................................... 16
5.2 Dealing with Spills............................................................................................................. 16
Troubleshooting............................................................................................................................ 17
Reference...................................................................................................................................... 18
1
Symbols
ConformitéEuropéenne
EU authorized representative
Supplementary fuse
Grounded conductor
Date of manufacturing
Manufacturer
Catalogue number
Serial number
Alternative current
Caution, consult instruction for use
WEEE symbol
This product is manufactured in compliance with the European
Union’s Waste Electrical & Electronic Equipment (WEEE)
Directive 2012/19/EU. Please follow your institutional
requirements for disposal of the accessories.
2
Section 1. Introduction
The POCKIT™Nucleic Acid Analyzer (POCKIT) is designed for performing insulated
isothermal polymerase chain reaction (iiPCR) (Chang et al., 2012; Tsai et al., 2012). It provides
iiPCR-based analysis and is equipped with two optical channels (520 nm, 550 nm) for multiplex
detection. The qualitative test results are readily displayed on the LCD monitor, and
automatically saved in a SD card. It generates results for up to 8 nucleic acid samples within 1
hour.
1.1 Intended Use
POCKIT is intended to provide qualitative detection of nucleic acid targets using fluorescence-
based iiPCR reagents. Intended users are properly-trained laboratory technicians familiar with
molecular biology techniques, and those who want to perform iiPCR or its relative reagent kits
produced by GeneReach.
This instrument is intended for research purpose.
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Section 2. General Information
2.1 Product Components
POCKIT™Nucleic Acid Analyzer × 1 unit
User Manual × 1 copy
Warranty Card × 1 copy
Holder × 1 piece
2.2 Specifications
Dimensions: 280 mm (W) × 250 mm (D) × 85 mm (H)
Net Weight: 2.1 kg
Power Supply: 100-120/200-240 VAC, 50/60 Hz, 2A
Fuses: 2A, 250VAC, 5 × 20 mm fast-acting, low breaking capacity glass tube fuses.
Operating Temperature: 15 ~ 35°C
2.3 Testing Capacity
Number of Samples: 1 ~ 8 reactions per run
Reaction Volume: 45 ~ 55 l per reaction
2.4 Equipments and Materials Required But Not Provided
-Nucleic acid extraction kit
-Nucleic acid amplification reagents and kits
-200 l and 1000 l micropipette, tips, and 1.5 ml tubes
-R-tubes and Caps
-cubee™mini-centrifuge
-Disposable gloves
*Note: To avoid power failure, connecting POCKIT to an uninterrupted power system (UPS)
and/or voltage regulator is highly recommended.
4
2.5 Front View
No
Item
Description
1
Lid
Isolates the heat source, and prevents light interference on the
reaction chamber.
2
Reaction well
Accommodate the R-tube holder to perform the amplification.
3
Cooling vents
Releases the heat generated during reaction.
4
Control panel
A touchscreen where users key in the operation commands
and view the results.
5
SD card slot
A slot where users insert the SD card to run POCKIT and
save reaction raw data.
6
Holder
Load R-tubes into the reaction wells.
②
③
④
⑥
①
⑤
5
2.6 Rear View
No.
Item
Description
1
Main switch
To turn on/off the main power
2
Socket
To connect to the power cord
2.7 Software Version
No.
Item
Description
1
Software Version
Current software version on POCKIT system.
②
①
①
6
Section 3. Important Notes
Check the device upon receiving. If any items are missing or harmed, please contact your local
distributor or GeneReach immediately for replacement. Do not use any damage items as they
may lead to poor performance of the test or harm the user.
3.1 Safety Information
DO NOT OPEN LID
DURING REACTION
Please carefully read these warnings, precautions and safety/operating instructions
completely before operating this product.
Electromagnetic Compatibility: This product complies with the emissions and immunity
requirements of IEC 61326. It is advisable to evaluate the electromagnetic environment prior
to operating the device. Do not use this device in close proximity to sources of strong
electromagnetic radiation (for example, unshielded intentional radio frequency (RF) sources).
Strong electromagnetic radiation may interfere with the proper operation of the device.
Transport condition at -29 ~ 50°C, 55 ± 20% R.H.
Storage condition at 10 ~ 40°C, 55 ± 20% R.H.
Operation altitude: under 5000 m.
Do not touch any plug and electric switch with wet hands.
This product should be placed in a dry, clean and ventilated indoor place. Strictly keep away
from moisture.
Switch off and unplug the device before moving it.
Hold the plug while pulling the power cord out from the electrical outlet. Do not pull it by
the cord directly.
Do not place the power cord on any hot surfaces.
Pack the device carefully before moving and delivering. Vibration or crash may cause damage.
Users should revalidate the device after it has been moved.
Do not look directly into the LED light in the reaction wells.
Only use consumables and accessories provided from GeneReach.
Make sure the ambient temperature during reaction is kept at 15 ~ 35°C.
SD card should not be removed until the device is switched off.
Do not disassemble the device if any problem occurs. Contact your local distributor or
GeneReach directly for troubleshooting.
In case of emergency or abnormal conditions, turn off the power switch or unplug. Do not
block access to power switch or plug.
When working around the device, always wear a lab coat, disposable gloves and protective
goggles.
Waste materials, such as gloves and R-tube, may lead to contamination of other experiments.
Please discard them carefully according to your local regulation.
7
Section 4. Operation
4.1 Switch On
Plug in the POCKIT and switch on the main power located in the back. The instrument will
perform a self-test.
*Note: If self-testing runs longer than 15 minutes, please see Troubleshooting.
Caution: Make sure the power plug is connected to a grounded receptacle to prevent
electrical shock or fire.
4.2 Choose Detection Wavelength
1) After self-testing, choose the detection wavelength according to the kits or methods used and
press “OK”.
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2) “System Ready” will be shown after choosing the detection wavelength.
3) Before the reaction starts, to change the detection wavelength, press “CH Select” and
choose again.
9
4.3 Set up Date and Time
1) Press “Time Setting”.
2) Touch the numbers that needs to be changed on the screen. Press “Up” and “Down” buttons
to select the desired number. Press “OK” to confirm and return to the “System Ready”screen.
Press “Cancel” to quit and leave the “Time Setting” screen.
*Note: The data derived from each test is stored in the SD card and in a folder named after
the device’s default date and time.
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4.4 Run iiPCR Reaction
1) Place R-tubes into a holder.
2) Place the holder with R-tubes into the reaction well. Close the lid and press “Run” to start
the reaction.
*Note: Ensure the holder with R-tubes completely fits into the reaction wells.
Caution: Do not look directly into LED lights in the reaction wells.
3) The screen will display the remaining reaction time and the folder name of the reaction.
Caution: Should any of the circumstances below occur, the results are unreliable:
The lid is opened or the SD card is removed during the POCKIT reaction.
The ambient temperature is not in the range of 15-35°C during the reaction.
The variation of ambient temperature is greater than 5°C during the reaction.
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4) When the reaction complete, a beeping tone is made from POCKIT. Press “Buzzer OFF” to
turn off the sound.
*Note: If there is no beep sound after the completion of the reaction, please see
Troubleshooting.
5) If the reaction temperature is abnormal during the reaction, “Temperature Warning” will be
shown on the screen.
Caution: Fluctuation of ambient temperature should not be more than 5C during
the reaction.
12
4.5 Display the Result
1) An example of results displayed at the end of a single-wavelength (either 520 nm or 550 nm)
detection program.
2) An example of results displayed at the end of a double-wavelength (520 nm + 550 nm)
detection program.
3) Description of the results:
Results
Description
Positive
Negative
Undetermined
Warning
*Note: Please interpret the results according to the user manual of the kits used.
*Note: When you see a " " symbol, please contact your local distributors or GeneReach for
assistance.
13
4.6 Review the Raw Data
1) Insert the SD card on a PC or Mac. Find the raw.csv file in the folder.
2) Please refer to the kit manual for data interpretation.
4.7 Exit the Experiment
1) Press “Abort”to pause the reaction.
2) Press “Yes”to confirm and return to the self-testing screen, or press “No” to continue the
reaction.
14
4.8 Errors Occurred During the Operation
If any Errors listed below are shown, please see Troubleshooting.
15
4.9 Switch Off
After the reaction, switch off the main power and transfer the SD card to a PC or Mac to read
the raw data.
Caution: Ensure the reaction has been completed before shutting down the device.
Main power
16
Section 5. Cleaning and Maintenance
5.1 General Care
Use only clean and wet paper towel or cotton swab to wipe off stains in the reaction chamber.
Do not disassemble the POCKIT for cleaning as it will void the warranty. Do not use solvent,
detergent or bleach to clean the POCKIT surface.
*Note: Always wear gloves, and always switch off and unplug the POCKIT before cleaning.
5.2Dealing with Spills
1) Wipe off visible spills with disposable paper towel immediately, and contact your local
distributor or GeneReach directly for further assistance.
2) Please use ultraviolet lights (UV) to irradiate POCKIT for 15 minutes.
17
Troubleshooting
Observation or Problems
Comments and Suggestions
(a)
Self test runs more than 15 minutes
Re-start the device.
Contact your local distributors or GeneReach for
assistance.
(b) System crash
Re-start the device, prepare new reagents in R-tube,
and repeat the reaction.
If any further problems occur, please contact your
local distributor or GeneReach for assistance.
(c) SD card error
Re-insert SD card, or restart device.
Contact your local distributor or GeneReach for
assistance if the problem persists.
(d) No display on screen
Make sure the plug has been connected to the power
system.
Replace fuse.
Contact your local distributor or GeneReach for
assistance.
(e) System error
Contact your local distributor or GeneReach for
assistance.
(f) Temperature error
Keep ambient temperature between 15-35°C during
reaction.
Contact your local distributor or GeneReach for
assistance.
(g) Camera error
Contact your local distributor or GeneReach for
assistance.
(h) Time sequencing error
Contact your local distributor or GeneReach for
assistance.
(i) Create data directory error
Check storage space is available in SD card.
Keep number of files/folders below 512 in each
directory.
Contact your local distributor or GeneReach for
assistance.
(j) No beep sound
Contact your local distributor or GeneReach for
assistance.
(k) " " symbol shown after the run
Contact your local distributor or GeneReach for
assistance.
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Reference
1. Chang, H. G., Tsai, Y., Tsai, C., Lin, C., Lee, P., Teng, P., et al. (2012). Athermally baffled device
for highly stabilized convective PCR. Biotechnology Journal, 7(5), 662-666. doi: 10.1002/
biot.201100453
2. Tsai, Y., Lin, Y., Chou, P., Teng, P., Lee, P., et al. (2012). Detection of white spot syndrome virus
by polymerase chain reaction performed under insulated isothermal conditions. J. Virol. Methods
181:134-137.
3. Tsai, Y., Wang, H. T., Chang, H. G., Tsai, C., Lin, C., Teng, P., et al. (2012). Development of
TaqMan Probe-Based Insulated Isothermal PCR (iiPCR) for Sensitive and Specific On-Site
Pathogen Detection. PLoS ONE, 7(9), e45278. doi:10.1371/journal.pone.0045278
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