GenScript EStain User manual

Version 2.0

June 4, 2010

Protein Staining System

User Manual

For electric staining of proteins in mini polyacrylamide gels

The Biology CRO

GenScript USA Inc.

120 Centennial Ave.

Piscataway, NJ 08854

Tel: 732-885-9188, 732-885-9688

Fax: 732-210-0262, 732-885-5878

Email: [email protected]

Table of Contents

Product Contents

Product Specifications

eStainTM Protein Staining Device

Accessory Products

Introduction

Quick Reference Guide

Protocols

Troubleshooting

Examples of Results

Technical Support

Warranty

eStainTM Protein Staining System www.genscript.com

Product Contents Product Contents, continued

Type of products

eStainTM Protein Staining

Device Contents

Upon Receiving

the Instrument

eStainTM Protein Staining

Pads

eStainTM Protein Staining

Pads, continued

Product

eStainTM Protein Staining Device

Cat. No.

L02010

Component

eStainTM Protein Staining Device

Specific Power Cord based on the type

of unit ordered (for U.S./Canada/China/

Taiwan/Japan, Europe, or UK)

Forceps

Shovel

eStainTM Graphite Electrode (11mm)

(installed inside the device)

Ice Pack

Quantity

1 each

Product

eStainTM Protein Staining Pads (R-250, 20-pak)

eStainTM Protein Staining Pads (G-250, 20-pak)

Cat. No.

L02011

L02012

This manual is supplied with the following products:

The contents of the eStainTM Protein Staining Device are listed below

Examine the unit carefully for any damage incurred during transit. File any

damage claims with the carrier. The warranty does not cover in-transit

damage.

The following eStainTM Protein Staining Pads are available from GenScript:

If you ordered the eStainTM Protein Staining Pads, you will receive the

components listed in the table below. Store the eStainTM Protein Staining

Pads at room temperature. For best results, use the eStainTM Staining Pads

before the expiration date printed on the package.

eStainTM Graphite

Electrode Product

eStainTM Graphite Electrode (11mm, 1-pak)

Cat. No.

L02014

The following eStainTM Graphite Electrode is available from GenScript:

For best results, when a Graphite Electrode was used for 200 times of electric

staining, replace it with a new one.

Component

The eStainTM Protein Staining Pads

(R-250, 20-pak) contains:

eStainTM Protein Staining Pad (R-250)

Gel Window (66mm×56mm)

Gel Window (76mm×64mm)

The eStainTM Protein Staining Pads

(G-250, 20-pak) contains:

eStainTM Protein Staining Pad (G-250)

Gel Window (66mm×56mm)

Gel Window (76mm×64mm)

each pack eStainTM Protein Staining Pad

(R-250) contains:

1×eStainTM Cathode Pad (R-250)

1×eStainTM Anode Pad

each pack eStainTM Protein Staining Pad

(G-250) contains:

1×eStainTM Cathode Pad (G-250)

1×eStainTM Anode Pad

eStainTM Protein Staining System www.genscript.com eStainTM Protein Staining System www.genscript.com

Quantity

- 2 --1 -

Product Contents Product Contents, continued

Type of products

eStainTM Protein Staining

Device Contents

Upon Receiving

the Instrument

eStainTM Protein Staining

Pads

eStainTM Protein Staining

Pads, continued

Product

eStainTM Protein Staining Device

Cat. No.

L02010

Component

eStainTM Protein Staining Device

Specific Power Cord based on the type

of unit ordered (for U.S./Canada/China/

Taiwan/Japan, Europe, or UK)

Forceps

Shovel

eStainTM Graphite Electrode (11mm)

(installed inside the device)

Ice Pack

Quantity

1 each

Product

eStainTM Protein Staining Pads (R-250, 20-pak)

eStainTM Protein Staining Pads (G-250, 20-pak)

Cat. No.

L02011

L02012

This manual is supplied with the following products:

The contents of the eStainTM Protein Staining Device are listed below

Examine the unit carefully for any damage incurred during transit. File any

damage claims with the carrier. The warranty does not cover in-transit

damage.

The following eStainTM Protein Staining Pads are available from GenScript:

If you ordered the eStainTM Protein Staining Pads, you will receive the

components listed in the table below. Store the eStainTM Protein Staining

Pads at room temperature. For best results, use the eStainTM Staining Pads

before the expiration date printed on the package.

eStainTM Graphite

Electrode Product

eStainTM Graphite Electrode (11mm, 1-pak)

Cat. No.

L02014

The following eStainTM Graphite Electrode is available from GenScript:

For best results, when a Graphite Electrode was used for 200 times of electric

staining, replace it with a new one.

Component

The eStainTM Protein Staining Pads

(R-250, 20-pak) contains:

eStainTM Protein Staining Pad (R-250)

Gel Window (66mm×56mm)

Gel Window (76mm×64mm)

The eStainTM Protein Staining Pads

(G-250, 20-pak) contains:

eStainTM Protein Staining Pad (G-250)

Gel Window (66mm×56mm)

Gel Window (76mm×64mm)

each pack eStainTM Protein Staining Pad

(R-250) contains:

1×eStainTM Cathode Pad (R-250)

1×eStainTM Anode Pad

each pack eStainTM Protein Staining Pad

(G-250) contains:

1×eStainTM Cathode Pad (G-250)

1×eStainTM Anode Pad

eStainTM Protein Staining System www.genscript.com eStainTM Protein Staining System www.genscript.com

Quantity

- 2 --1 -

- 4 -

Product Specifications Product Specifications, continued

eStainTM Protein Staining

Device Specifications

- 3 -

eStainTM Graphite

Electrode Specifications

Weight:

Dimensions:

Electrical Parameters:

Built-in Features:

Compatibility:

Materials:

Operating Temperature:

Forceps:

Shovel:

2.3 kg

200 mm (l) ×180 mm (w) ×90 mm (h)

100-120 V, 220-240 V, 50/60 Hz, 3.2 A

Digital display, alarm, light LED

Suitable for electric staining of proteins in mini

polyacrylamide gels

Acrylonitrile Butadiene Styrene, Polycarbonate,

Aluminum, Titanium, Plasticized silicone.

15 - 40°C

Stainless steel

Polycarbonate

Dimensions:

Weight:

Materials:

100 mm (l) ×90 mm (w) ×11mm (h)

180g

Powdered carbon, clay

eStainTM Graphite Electrode (11mm)

The eStainTM Graphite Electrode is used as the replaceable anode of eStainTM

Protein Staining Device and available separately from GenScript.

The specifications for eStainTM Graphite Electrode are listed below:

eStainTM Protein Staining

Pads Specifications

size:

Materials:

8cm (l) x 7cm (w) x2.5mm (thick)

Blotting filter paper presoaked with proprietary

cathode buffer containing CBB R-250 or G-250

size:

Materials:

8cm (l) x 7cm (w) x2.5mm (thick)

Blotting filter paper presoaked with proprietary

anode buffer

The eStainTM Protein Staining Pads are used with the eStainTM Protein Staining

Device.

The specifications of the eStainTM Protein Staining Pads are listed below:

eStainTM Cathode Pad (R-250 or G-250)

Avoid acetone, dimethyl sulfoxide, and acetic acid. These reagents can erode or

damage the device.

eStainTM Anode Pad

Inter frame size:

Materials:

66mm×56mm

76mm×64mm

Polycarbonate

Gel Window

eStainTM Protein Staining System www.genscript.com eStainTM Protein Staining System www.genscript.com

- 4 -

Product Specifications Product Specifications, continued

eStainTM Protein Staining

Device Specifications

- 3 -

eStainTM Graphite

Electrode Specifications

Weight:

Dimensions:

Electrical Parameters:

Built-in Features:

Compatibility:

Materials:

Operating Temperature:

Forceps:

Shovel:

2.3 kg

200 mm (l) ×180 mm (w) ×90 mm (h)

100-120 V, 220-240 V, 50/60 Hz, 3.2 A

Digital display, alarm, light LED

Suitable for electric staining of proteins in mini

polyacrylamide gels

Acrylonitrile Butadiene Styrene, Polycarbonate,

Aluminum, Titanium, Plasticized silicone.

15 - 40°C

Stainless steel

Polycarbonate

Dimensions:

Weight:

Materials:

100 mm (l) ×90 mm (w) ×11mm (h)

180g

Powdered carbon, clay

eStainTM Graphite Electrode (11mm)

The eStainTM Graphite Electrode is used as the replaceable anode of eStainTM

Protein Staining Device and available separately from GenScript.

The specifications for eStainTM Graphite Electrode are listed below:

eStainTM Protein Staining

Pads Specifications

size:

Materials:

8cm (l) x 7cm (w) x2.5mm (thick)

Blotting filter paper presoaked with proprietary

cathode buffer containing CBB R-250 or G-250

size:

Materials:

8cm (l) x 7cm (w) x2.5mm (thick)

Blotting filter paper presoaked with proprietary

anode buffer

The eStainTM Protein Staining Pads are used with the eStainTM Protein Staining

Device.

The specifications of the eStainTM Protein Staining Pads are listed below:

eStainTM Cathode Pad (R-250 or G-250)

Avoid acetone, dimethyl sulfoxide, and acetic acid. These reagents can erode or

damage the device.

eStainTM Anode Pad

Inter frame size:

Materials:

66mm×56mm

76mm×64mm

Polycarbonate

Gel Window

eStainTM Protein Staining System www.genscript.com eStainTM Protein Staining System www.genscript.com

- 6 -

eStainTM Protein Staining Device eStainTM Protein Staining Device, continued

Front View of

eStainTM Device

Rear View of

eStainTM Device

- 5 -

Control Panel of

eStainTM Device

Inside View of

eStainTM Device

The control panel of the eStainTM Protein Staining Device is described below.

When upper status light is on, which indicates staining mode, the Digital

Display shows running time; otherwise when both upper and lower status lights

are on, which indicates numbering mode, the Digital Display shows how many

times of electric staining the eStainTM Graphite Electrode has been used for.

The Reset button is used to clear parameters.

The Time button is used to set running time and toggle between staining mode

and numbering mode.

The Start/Stop button is used to activate/stop the staining program.

The inside view showing various parts of the eStainTM Protein Staining

Device is shown below:

The rear view showing various parts of the eStainTM Protein Staining Device

is shown below.

The front top view showing various parts of the eStainTM Protein Staining Device

is shown below.

Control Panel

Handle Bar

eStainTM Protein Staining System www.genscript.com eStainTM Protein Staining System www.genscript.com

Power Switch AC Inlet Titanium Cathode

Graphite Anode

- 6 -

eStainTM Protein Staining Device eStainTM Protein Staining Device, continued

Front View of

eStainTM Device

Rear View of

eStainTM Device

- 5 -

Control Panel of

eStainTM Device

Inside View of

eStainTM Device

The control panel of the eStainTM Protein Staining Device is described below.

When upper status light is on, which indicates staining mode, the Digital

Display shows running time; otherwise when both upper and lower status lights

are on, which indicates numbering mode, the Digital Display shows how many

times of electric staining the eStainTM Graphite Electrode has been used for.

The Reset button is used to clear parameters.

The Time button is used to set running time and toggle between staining mode

and numbering mode.

The Start/Stop button is used to activate/stop the staining program.

The inside view showing various parts of the eStainTM Protein Staining

Device is shown below:

The rear view showing various parts of the eStainTM Protein Staining Device

is shown below.

The front top view showing various parts of the eStainTM Protein Staining Device

is shown below.

Control Panel

Handle Bar

eStainTM Protein Staining System www.genscript.com eStainTM Protein Staining System www.genscript.com

Power Switch AC Inlet Titanium Cathode

Graphite Anode

- 8 -

Accessory Products Introduction

eStainTM Protein Staining System www.genscript.com

eStainTM Protein Staining

Pads

eStainTM Graphite

Electrode

Additional Products

- 7 -

eStainTM Protein Staining System www.genscript.com

System Overview

System Components

The eStainTM Protein Staining System is a unique electric staining system that

allows you to quickly, reliably and efficiently stain proteins in various types of

mini polyacrylamide gels with Coomassie blue dye reagents. It consists of the

eStainTM Protein Staining Device and eStainTM Protein Staining Pads.

The proprietary electric staining technology of the eStainTM Protein Staining

Device combined with the eStainTM Protein Staining Pads applies a high

electric force generated between graphite anode and titanium cathode to

allow for quick and directional movement of negatively charged Coomassie

blue dye reagents into the gel matrix to stain the proteins and also the quick

and directional movement of the free staining reagents out of the gel matrix to

destain the gel matrix within only 7 minutes or less. The eStainTM Protein

Staining System integrates three steps of conventional Coomassie blue

staining method into a single step and greatly cuts down the time required for

protein staining analysis. The proteins stained using the eStainTM Protein

Staining System exhibit as high detection sensitivity as proteins stained using

conventional Coomassie blue staining method.

The eStainTM Protein Staining System consists of:

eStainTM Protein Staining Device

The eStainTM Protein Staining Device is a user-friendly electric staining unit

that allows for fast, convenient and efficient in-gel protein staining with

Coomassie blue dye reagents.

eStainTM Protein Staining Pads

The eStainTM Protein Staining Pads are disposable pads to perform electric

staining of proteins in gels with Coomassie blue dye. Two types of eStainTM

Protein Staining Pad, R-250 and G-250, are available separately from

GenScript and for different requirements. Each pack eStainTM Protein Staining

Pad contains an eStainTM Cathode Pad presoaked with proprietary cathode

buffer containing CBB dye R-250 or G-250, and an eStainTM Anode Pad

presoaked with proprietary anode buffer, allowing for rapid, convenient and

reliable in-gel protein staining without the need to prepare any solutions and

buffers.

eStainTM Graphite Electrode is available separately from GenScript. Ordering

information is provided below.

The precast ExpressTM PAGE Gels as well as premade buffers or buffer powders

are available from GenScript. For details, contact Technical Support or visit

www.genscript.com.

eStainTM Protein Staining Pads are available separately from GenScript. Ordering

information is provided below.

Product

eStainTM Protein Staining Pads

(R-250, 20-pak)

eStainTM Protein Staining Pads

(G-250, 20-pak)

Cat. No.

L02011

L02012

Quantity

1 box

Product

eStainTM Graphite Electrode (11mm, 1-pak)

Cat. No.

L02014

Quantity

1 unit