Hanna Instruments HI 96759C User manual

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INSTRUCTION MANUAL
HI96759C
Maple Syrup Transmittance Analyzer

All rights are reserved. Reproduction in whole or in part is prohibited without the written consent
of the copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895, USA.
Thank you for choosing a Hanna Instruments product.
Please read this instruction manual carefully before using the instrument.
This manual will provide you with the necessary information for correct use of
the instrument, as well as a precise idea of its versatility.
If you need additional technical information, do not hesitate to e‑mail us at
[email protected] or view our worldwide contact list at www.hannainst.com.
Dear
Customer,

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TABLE OF CONTENTS
PRELIMINARY EXAMINATION ............................................................................................
GENERAL DESCRIPTION ....................................................................................................
ABBREVIATIONS ..............................................................................................................
SIGNIFICANCE AND USE....................................................................................................
SPECIFICATIONS...............................................................................................................
PRINCIPLE OF OPERATION ...............................................................................................
FUNCTIONAL DESCRIPTION ...............................................................................................
ERRORS AND WARNINGS .................................................................................................
GENERAL TIPS FOR AN ACCURATE MEASUREMENT .............................................................
STARTUP ........................................................................................................................
MEASUREMENT PROCEDURE ............................................................................................
BATTERY MANAGEMENT ...................................................................................................
BATTERY REPLACEMENT ...................................................................................................
ACCESSORIES ..................................................................................................................
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Please examine this product carefully. Make sure that the instrument is not damaged. If any damage
occurred during shipment, please contact your local Hanna Instruments Office.
Each HI96759 Ion Selective Meter is supplied complete with:
• Six Sample Cuvettes
• One Light Shield Cap
• 5 mL Syringes (2 pcs.)
• 30 mL Glycerol (1 bottle)
• 9V Battery
• Cloth for wiping cuvettes
• Instrument Quality Certificate
• Instruction Manual and Quick Reference Guide
• Rigid carrying case
Note: Save all packing material until you are sure that the instrument works correctly. Any
defective item must be returned in its original packing.
PRELIMINARY EXAMINATION

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The HI96759 is an auto diagnostic portable microprocessor meter that benefits from
Hanna Instruments’ years of experience as a manufacturer of analytical instruments. It has the
advanced optical system based on a special tungsten lamp and a narrow band interference filter
that allows most accurate and repeatable readings. All instruments are factory calibrated and the
electronic and optical design minimizes the need of frequent calibration.
All instruments are splash proof and the lamp and filter units are protected from dust or dirt. This
makes the instruments fulfill field applications. Display messages aid the user in routine operation.
The meter has an auto‑shut off feature that will turn off the instrument after 10 minutes of non
use in measurement mode or after 1 hour if left in calibration mode.
The meter uses an exclusive positive‑locking system to ensure that the cuvette is in the same position
every time it is placed into the measurement cell.
The HI96759 measures the percent light transmittance of maple syrup compared to analytical grade
glycerol. The transmittance value allows identification of the syrup quality class.
GENERAL DESCRIPTION
degree Celsius
degree Fahrenheit
milliliter
millivolt
Liquid Crystal Display
°C:
°F:
mL:
mV:
LCD:
ABBREVIATIONS

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SIGNIFICANCE AND USE
Color class Range (% Tansmittance)
Grade A Light Amber 75.0 to 100.0
Grade A Medium Amber 60.5 to 74.9
Grade A Dark Amber 44.0 to 60.4
Grade B for reprocessing 27.0 to 43.9
Substandard less than 27.0
The primary characteristic for classifying maple syrup is color. When syrup is very light in color, as
matching the minimum light transmittance standards, the grade assigned to syrup is high. When
syrup color is dark, the grade is low. Color classes are expressed in percent of light transmission as
compared to an analytical Glycerol Standard Reference fixed at one hundred percent transmission.
All syrup is considered to be at minimum density, 66.0 degrees Brix at 68 °F (20 °C), and to have
no flavors or other characteristics extraneous to pure maple syrup.

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SPECIFICATIONS
Range 0.0 to 100.0 % Transmittance
Resolution 0.1 % Transmittance
Accuracy
@25 ˚C (77 ˚F) ±3 % @75.0 % Transmittance
Light Source Tungsten Lamp
Light Detector Silicon Photocell with narrow band interference filter @560 nm
Method Direct Measurement
Environment 0 to 50 °C (32 to 122 °F);
max 95% RH non‑condensing
Battery Type 9V (1 pc.)
Auto‑Shut off
After 10’ of non‑use in measurement mode;
after 1 hour of non‑use in calibration mode;
with last reading reminder
Dimensions 192 x 104 x 69 mm (7.6 x 4.1 x 2.7”)
Weight 320 g (11.3 oz.)
REQUIRED REAGENTS
Code Description Quantity/test
HI93703‑57 Glycerol 4 mL

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Absorption of Light is a typical phenomenon of interaction between electromagnetic radiation and
matter. When a light beam crosses a substance, some of the radiation may be absorbed by atoms,
molecules or crystal lattices.
If pure absorption occurs, the fraction of light absorbed depends both on the optical path length
through the matter and on the physical‑chemical characteristics of the substance according to the
Lambert‑Beer Law:
‑log I/Io= ελc d
or
A = ελc d
Where:
‑log I/Io = Absorbance (A)
Io = intensity of incident light beam
I = intensity of light beam after absorption
ε
λ= molar extinction coefficient at wavelength λ
c = molar concentration of the substance
d = optical path through the substance
Therefore, the concentration “c” can be calculated from the absorbance of the substance as the
other factors are known.
Photometric chemical analysis is based on the possibility to develop an absorbing compound from a
specific chemical reaction between sample and reagents. Given that the absorption of a compound
strictly depends on the wavelength of the incident light beam, a narrow spectral bandwidth should
be selected as well as a proper central wavelength to optimize measurements.
The optical system of Hanna Instruments’ HI96 series colorimeter is based on special subminiature
tungsten lamps and narrow‑band interference filters to guarantee both high performance and
reliable results.
HI96 series block diagram (optical layout)
PRINCIPLE OF OPERATION

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PRINCIPLE OF OPERATION
A microprocessor controlled special tungsten lamp emits radiation which is first optically conditioned
and beamed to the sample contained in the cuvette. The optical path is fixed by the diameter of
the cuvette. Then the light is spectrally filtered to a narrow spectral bandwidth, to obtain a light
beam of intensity Ioor I.
The photoelectric cell collects the radiation Ithat is not absorbed by the sample and converts it
into an electric current, producing a potential in the mV range.
The microprocessor uses this potential to convert the incoming value into the desired measuring
unit and to display it on the LCD.
The measurement process is carried out in two phases: first the meter is zeroed and then the actual
measurement is performed.
The cuvette has a very important role because it is an optical element and thus requires particular
attention. It is important that both, the measurement and the calibration (zeroing) cuvettes, are
optically identical to provide the same measurement conditions. Whenever possible use the same
cuvette for both. It is necessary that the surface of the cuvette is clean and not scratched. This to avoid
measurement interference due to unwanted reflection and absorption of light. It is recommended
not to touch the cuvette walls with hands.
Furthermore, in order to maintain the same conditions during the zeroing and the measuring phases,
it is necessary to close the cuvette to prevent any contamination.

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DISPLAY ELEMENTS DESCRIPTION
1) The measuring scheme (lamp, cuvette, detector), appears during different phases of zero or
reading measurement
2) Error messages and warnings
3) The battery icon indicates the charge state of the battery
4) The hourglass appears when an internal checking is in progress
5) Four digit main display
6) Measuring units
KEYPAD DESCRIPTION
• ON/OFF: to turn the meter on and off.
• CAL: to calibrate the meter prior to measurement.
• READ: to make a measurement.
OPERATING MODES
• Measurement mode: default operation mode, enables both calibration and measurement.
FUNCTIONAL DESCRIPTION
INSTRUMENT DESCRIPTION
1) Liquid Crystal Display (LCD)
2) CAL key
3) READ key
4) ON/OFF key
5) Cuvette alignment indicator
6) Cuvette holder