Harvard Apparatus QuikPrep ElectroPrep 74-1196 User manual

Publicaon 9511-073 REV-1.0
ElectroPrep™System
USER’S MANUAL
ElectroPrep System 74-1196

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ElectroPrep System
Publicaon 9511-073 REV-1.0
Table of Contents
SUBJECT PAGE #
Warranty Informaon 2-3
Safety Informaon 4-5
Product Overview 6
Using the ElectroPrep Unit 7-11
Assembly and Use 7-9
How to Select Your Chamber and
Membrane Conguraon 10-11
ElectroPrep Protocols 12-15
Electrodialysis through Simultaneous
Exchange of Buers 12
Selecve Electroltraon/Concentraon/Separaon
Based on Dierent Charges of Biomolecules 13
Rapid and Selecve Electroltraon or Concentraon 14
Electroeluon and Electrofraconaon of DNA,
Proteins or Other Biomolecules from Gel Pieces 15
Specicaons 16
General Care & Maintenance 17
Ordering Informaon 18-21

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ElectroPrep System
Publicaon 9511-073 REV-1.0
Warranty Information
RESEARCH USE ONLY
Harvard Apparatus
84 October Hill Rd
Holliston, MA 01746, USA
Phone: [phone]
Fax: [phone]
Web: www.harvardapparatus.com
Warranty
Harvard Apparatus warranes the QuikPrep ElectroPrep System for a period of one year
from the date of purchase. At its opon, Harvard Apparatus will repair or replace the
unit if it is found to be defecve as to workmanship or materials. This warranty does not
extend to any instrumentaon which has been (a) subjected to misuse, neglect, accident
or abuse, (b) repaired or altered by anyone other than Harvard Apparatus without
Harvard Apparatus express and prior approval, (c) used in violaon of instrucons
furnished by Harvard Apparatus. This warranty extends only to the original customer
purchaser. IN NO EVENT SHALL HARVARD APPARATUS BE LIABLE FOR INCIDENTAL
OR CONSEQUENTIAL DAMAGES. Some states do not allow exclusion or limitaon of
incidental or consequenal damages so the above limitaon or exclusion may not apply
to you. THERE ARE NO IMPLIED WARRANTIES OF MERCHANTABILITY, OR FITNESS
FOR A PARTICULAR USE, OR OF ANY OTHER NATURE. Some states do not allow this
limitaon on an implied warranty, so the above limitaon may not apply to you. Without
liming the generality of the foregoing, Harvard Apparatus shall not be liable for any
claims of any kind whatsoever, as to the equipment delivered or for non-delivery of
equipment, and whether or not based on negligence. Warranty is void if the instrument
is changed in any way from its original factory design or if repairs are aempted without
wrien authorizaon by Harvard Apparatus. Warranty is void if parts, connecons not
manufactured by Harvard Apparatus are used with the QuikPrep ElectroPrep System.
If a defect arises within the warranty period, promptly contact Harvard Apparatus,
84 October Hill Road, Holliston, Massachuses, USA 01746-1388 by phone at
800-547-6766 or 508-893-8999 or email at [email protected].
Goods will not be accepted for return unless an RMA (Returned Materials Authorizaon)
number has been issued by our returns/repairs department. The customer is responsible
for shipping charges. Please allow a reasonable period of me for compleon of repairs,
replacement and return. If the unit is replaced, the replacement unit is covered only for
the remainder of the original warranty period dang from the purchase of the original
device. This warranty gives you specic rights, and you may also have other rights, which
vary from state to state.

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ElectroPrep System
Publicaon 9511-073 REV-1.0
Warranty Information
Out of Warranty Service
Proceed exactly as for Warranty Service above. If our service department can assist you
by phone or other correspondence, we will be glad to help at no charge.
Repair service will be billed on the basis of labor and materials. A complete statement of
me spent and materials used will be supplied. Shipment to Harvard Apparatus should
be prepaid. Your bill will include return shipment freight charges.
Disassembly by the user is prohibited. Service should only be carried out by
experienced Harvard Apparatus technicians.
Repair Facilies and Parts
Harvard Apparatus stocks replacement and repair parts. When ordering, please describe
parts as completely as possible, preferably using our part numbers. If praccal, enclose a
sample photo or drawing.
Safety Precauons
• Always isolate ElectroPrep unit from the power supply before removing the safety
cover. Isolate the power supply from the main rst then disconnect the leads
• DO NOT exceed the maximum operang voltage or current.
• DO NOT operate the unit in metal trays.
• Following the replacement of electrodes, have the unit inspected and approved by
your safety ocer prior to use.
• DO NOT ll the unit with running buer above the maximum ll line.
• DO NOT move the unit when it is running
• CAUTION: During use very low quanes of various gases are produced at the
ElectroPrep electrodes. The type of gas produced depends on the composion of
the buer employed. To disperse these gases, make sure that the apparatus is run
in a well venlated area.

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ElectroPrep System
Publicaon 9511-073 REV-1.0
Please read the following safety precauons to ensure proper use of your generator.
If the equipment is used in a manner not specied, the protecon provided by the
equipment may be impaired.
To Prevent Hazard or Injury
Make Proper Connecons
Make sure all connecons are made properly and securely. Any signal wire
connecons to the unit must be no longer than three meters.
Observe All Terminal Rangs
Review the operang manual to learn the rangs on all connecons.
Avoid Exposed Circuitry
Do not touch any electronic circuitry inside of the product.
Do Not Operate with Suspected Failures
If damage is suspected on or to the product do not operate the product.
Contact qualied service personnel to perform inspecon.
Orient the Equipment Properly
Do not orient the equipment so that is dicult to manage the connecon
and disconnecon of devices.
Place Product in Proper Environment
Review the operang manual for guidelines for proper operang environments.
Observe all Warning Labels on Product
Read all labels on product to ensure proper usage.
If there are any quesons about the operaon of this instrument,
call Harvard Apparatus Technical Service at 800-272-2775, or 508-893-8999.
Safety Information

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ElectroPrep System
Publicaon 9511-073 REV-1.0
Safety Information
Cauon Noce
The QuikPrep ElectroPrep System is intended for laboratory use only and may be used in
research and development applicaons. These systems have been designed to meet the
applicable safety requirements for electrical equipment for measurement, control, and
laboratory use. The unit itself does not generate waste, but may be used to treat samples
that are hazardous. Please use appropriate PPE and ensure disposal in accordance with local
regulaons and pracces.
This product should not be used in the presence of a ammable atmosphere such as an
anesthec mixture with air, oxygen, or nitrous oxide.
Cauon Cauon Risk of
Electric Shock

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ElectroPrep System
Publicaon 9511-073 REV-1.0
Product Overview
The QuikPrep® ElectroPrep™ Electrodialysis System is an extremely versale patented
sample preparaon technology that is capable of separang samples by both size and
charge. It is ideal for the rapid puricaon of proteins, nucleic acids, carbohydrates and
other biomolecules. Membranes of dierent MWCO (molecular weight cut o), from
100 to 300,000 daltons, can be used for selecve buer exchange, dialysis, ltraon,
concentraon, fraconaon and eluon.
The ElectroPrep System provides faster dialysis mes due to movement of charged
molecules in an electric eld during dialysis, thus combining electrophoresis with dialysis.
With a run me of 5 to 10 minutes, ElectroPrep provides speed and convenience, even at
the very low currents (5 to 10 mA) used with this system.
Applicaons
• Electroeluon from gels and soluons, e.g. gel extracon of vector and
insert during cloning
• Electrodialysis (with an average buer exchange me of 5 to 10 minutes)
• Electroconcentraon
• Selecve electroltraon
• Size fraconaon of DNA and proteins from complex lysates
• Primer removal following PCR amplicaon
• Salt removal from DNA mini-preps
• Detergent removal
• Dye-terminator removal

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Publicaon 9511-073 REV-1.0
Using the ElectroPrep Unit
Assembly and Use
A funconal ElectroPrep System consists of the ElectroPrep Tank, power supply,
and one or more Dialyzer Units.
The ElectroPrep Tank (74-1196) is supplied with a tank, lid and connectors, and a
replacement gasket. Power supply and Dialyzer Units must be purchased separately.
The ElectroPrep System uses at least one Dialyzer Unit to perform a sample
electrodialysis. A basic Dialyzer Unit is comprised of a dialyzer chamber, dialysis
membranes at one or both ends of the chamber, and two end caps.
Example of basic Dialyzer Unit, unassembled, comprised of one dialysis chamber,
two membranes and two end caps.

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Publicaon 9511-073 REV-1.0
Using the ElectroPrep Unit
Dialyzer Units can be congured in a number of more complex ways to perform
dierent applicaons using a combinaon of components:
• Dialysis Chamber: the major receptacles for either samples or dialyzed materials.
Includes one main chamber with two open ports and two open end caps. The sample
chambers are made of PTFE, an inert material especially suited for high sample
recovery and are available in a range of 50 µl to 1500 µl volumes. All Dialysis
Chambers use 15/16” diameter membranes.
-Two end caps may be used, one at the end of each chamber
• Union: joins two dialysis chambers together
- Without membranes to make a larger volume chamber
- With dialysis membranes of appropriate MWCOs for serial dialysis. (The
juncon between a Dialysis Chamber and a Union accommodates the same
size 15/16” diameter membranes as the juncon between a Dialysis Chamber
and its end cap).
• Link Chambers: Link chambers may be used for concentraon of dialyzed samples
or for size fraconaon of samples using membranes of dierent MWCOs. As with
Unions, Link Chambers may also be connected to Chambers without membranes to
make a larger volume chamber. Each Link Chamber comes with one open end cap.
Primary and Secondary Link Chambers accept dierent size membranes at their
juncons on either side facing the Dialysis Chamber or the Link Chamber cap.
- Primary Link Chamber: can be joined directly to a dialysis chamber on one
end and joined to a cap or a secondary link chamber on the other end.
Primary link chambers are available in a range of 50 µl to 1500 µl volumes.
The juncon between a Dialysis Chamber and a Primary Link Chamber
accepts a 15/16” diameter membrane and the juncon between a Primary
link chamber and a Secondary Link Chamber or cap accepts an 11/16”
diameter membrane.
- Secondary Link Chamber: can be joined to a primary link chamber on one
end and can be joined to a cap on the other end. Secondary link chambers
are available in either 50 µl or 100 µl volumes. The juncon between a
Primary and Secondary Link Chambers accepts an 11/16” size membrane and
the juncon between a Secondary Link Chamber and its cap accepts a 7/16”
diameter membrane.

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ElectroPrep System
Publicaon 9511-073 REV-1.0
Using the ElectroPrep Unit
• Dialysis Membranes are added at one or both ends or between Dialysis Chamber
and/or Link Chambers and Unions. Membranes with molecular weight cut o
points (MWCOs), ranging from 500 to 300,000 daltons, may be used in combinaon
with dierent Dialysis and Link Chambers for selecve eluon, ltraon, dialysis,
fraconaon and concentraon of complex samples. Dialysis Membranes are available
in three sizes: 7/16”, 11/16”, and 15/16” Membrane diameters are available. The
Ordering Informaon at the end of this manual indicates what diameter membrane is
used for various components and chamber sizes.
NOTE: ElectroPrep dialysis chambers, unions, link chambers and membranes are
purchased separately. Components required depend on conguraon.

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ElectroPrep System
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Using the ElectroPrep Unit
Primary Link Chamber
Secondary Link
Dialysis Chamber
ElectroPrep Membrane
Open End Cap
Key
Open End Caps:
Lock membranes
into posion.
Membranes:
Facilitate selecve separaon
of biological samples from 500
to 300,000 Da.
Dialysis Chamber:
The major receptacle for
sample or dialyzed product.
Secondary Link Chamber:
Can be connected to a
Primary Link Chamber, and
joined with an Open End Cap
at the other end. Membranes
of dierent MCWOs may be
used at juncons of these
chambers and caps.
Primary Link Chamber:
Can be connected to a
Dialysis Chamber, and
joined with a Secondary Link
Chamber or Open End Cap at
the other end. Membranes
of dierent MCWOs may be
used at juncons of these
chambers and caps for size
fraconaon.
How to Select Your Chamber and Membrane Conguraon
1. Decide upon your applicaon, e.g., electrodialysis, electroeluon, electroltraon,
electroconcentraon, electroseparaon.
2. Select a Dialysis Chamber able to hold the desired sample volume (50 to 1500 µl.)
Note that two Dialysis Chambers can be joined with a Union (with or without
membranes added between the Union and Chambers) to increase sample
volume (up to 600 or 3500 µl).

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Publicaon 9511-073 REV-1.0
Using the ElectroPrep Unit
3. Choose Dialysis Membranes of suitable size, type and MWCO depending on the
applicaon being done and the molecular weight of the biological molecule of interest.
a. Membrane Type: Take into account the membrane’s suitability for use in
aqueous or organic solvents.
• For organic solvents, use either regenerated cellulose or polycarbonate
• For aqueous soluons, use cellulose acetate
b. Membrane Size: Refer to the Ordering Informaon (Pages 18-21) for what
membrane diameter you need for each component in your conguraon.
4. Assemble Dialysis Unit
• With one Dialysis Chamber, two membranes and two Open End Caps for desalng or
buer exchange (Conguraon #1)
• With two Dialysis Chambers of equal volume, three membranes, a Union, and two
Open End Caps for electroseparaon and electroeluon (Conguraon #2)
• With Dialysis Chamber, three membranes, a smaller volume Link Chamber, and two
Open End Caps for electroconcentraon or electroltraon (Conguraon #3)
• With Dialysis Chambers, six membranes of dierent MWCO, a Union, and mulple Link
Chambers for electrofraconaon (Conguraon #4)
• Note: Conguraons 1 to 4 are just a few examples of Electroprep Unit assembly.
Addional conguraons for electrofraconaon are possible using addional
combinaons of Dialysis Chambers, Unions, Primary Link Chambers, and Secondary
Link Chambers.
Most Basic:
To Desalt or Buer Exchange
Two Dierent Volume Chambers:
To Selecvely Concentrate
Larger Volume Chambers:
To Purify and Concentrate or Filter
Complex Conguraon:
For Concentraon/Filtraon/Separaon
Conguraon #1: Single Chamber with two
matching open-ended caps
Conguraon #3: Single chamber with primary
link and two open end caps.
Conguraon #2: Two chambers connected
with a union and enclosed with two matching
open end caps.
Conguraon #4: Two chambers connected
with a union, each using a primary link that is
enclosed with two matching open end caps.
Example Conguraons

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ElectroPrep System
Publicaon 9511-073 REV-1.0
ElectroPrep Protocols
Electrodialysis through Simultaneous Exchange of Buers
(Conguraon #1)
Applicaons
• Rapidly remove 100% of primer aer PCR reacon (5 to 10 minutes).
• De-salng of neutral materials that do not move in an electric eld (such as sugars)
or charged molecules at their isoelectric point.
In this conguraon, a sample is placed in the Dialysis Chamber between Membranes (a) and (b),
both of which have MWCOs lower than the molecular weight of the desired biomolecules. The
sample is dialyzed through the simultaneous exchange of buers in the electric eld. This method is
very fast and very eecve.
Steps
1. Select the proper size Dialyzer Chamber to use as much of the available chamber volume as
possible (minimize air gaps).
2. Chose membranes, (a) and (b), that have MWCOs lower than the molecular weight of the
biomolecule(s) of interest.
3. Assemble Dialyzer Unit by placing one membrane on the plaorm of the Dialyzer Chamber and
hand-ghtening one End Cap.
4. Place the sample in the Dialysis Chamber between the two membranes.
5. Place the second membrane and assemble the second End Cap to create the completed Dialysis
Chamber unit.
6. Install the Dialysis Chamber unit into the tank by gently pushing the unit through the ElectroPrep
tank gasket to secure in place.
7. Fill the Electroprep tank with buer and ensure the Dialysis Chamber is covered, but the buer does
not ow over the paron. Dialysis Chamber and caps must be completely immersed in buer so
that no air bubbles are present. Keeping all parts submersed in buer prevents the introducon of air
bubbles into the Dialyzer Chambers.
8. Assemble the lid with cables and connect to your power supply. Use current and voltage sengs as
required for the sample and buer (5 to 10 mA suggested as a starng point).
ElectroPrep Dialyzer
Open End Cap
Membrane
Key
Electrodialysis
through Simultaneous
Exchange of Buers

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ElectroPrep System
Publicaon 9511-073 REV-1.0
ElectroPrep Protocols
Selecve Electroltraon/Concentraon/Separaon Based on
Dierent Charges of Biomolecules (Variaon of Conguraon #2)
Applicaons
• Separaon and puricaon of biomolecules with unknown isoelectric potenal
In Conguraon #2 the sample is placed in a union between two membranes (b), both of which should
have a MWCO larger than the desired biomolecules. Membranes (a) and (c) should have MWCOs smaller
than the desired biomolecules.
Based on their charges, the desired biomolecules will move to either dialysis Chamber (1) or Chamber
(2), whereas the lowest molecular weight molecules will migrate through membranes (a) and (c) into
the tank. Biomolecules with unknown isoelectric points can also be separated and puried using this
method. Dialysis chambers of smaller volume can be used to concentrate samples.
Steps
1. Select the proper size Dialyzer Chamber and Union to use as much of the available chamber volume as
possible (minimize air gaps).
2. Assemble Dialyzer Unit by placing one membrane (a) on the plaorm of the Dialyzer Chamber and
hand-ghtening an End Cap.
3. Add buer to the Dialyzer chamber.
4. Place your next membrane (b) on the other side of the Dialyzer Chamber and assemble the Union.
5. Add your sample to the Union compartment and place your next membrane (b) and Dialyzer Chamber.
6. Add buer to the second Dialyzer Chamber, place the last membrane (c), and place End Cap
to create the completed Dialysis Chamber unit.
7. Gently push the Dialysis Chamber unit through the ElectroPrep tank gasket to secure the unit in place.
8. Fill the Electroprep tank with buer and ensure the Dialysis Chambers are covered, but the uid does
not ow over the paron. Chambers and caps must be completely immersed in buer so that no air
bubbles are present. Keeping all parts submersed in buer prevents the introducon of air bubbles
into the Dialyzer Chambers.
9. Assemble the lid with cables and connect to your power supply. Use current and voltage sengs as
required for the sample and buer (5 to 10 mA suggested as a starng point).



ElectroPrep Dialyzer
Open End Cap
Membrane
Union
Key
Selecve Electro-Filtraon/
Concentraon/Separaon
Based on Dierent Charges
on Biomolecules

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ElectroPrep System
Publicaon 9511-073 REV-1.0
ElectroPrep Protocols
Rapid and Selecve Electroltraon or Concentraon
(Variaon of Conguraon #2)
Applicaons
• Concentraon of small samples and/or selecve ltraon
In this Conguraon #2 variant, the sample is placed in the sample compartment comprised of a Dialysis
Chamber (1) and the Union connected to a receiving Dialysis Chamber (2) of same or smaller volume. The
MWCO of membrane (b) should be larger than the molecular weight of the biomolecules and the MWCO
of membranes (a) and (c) should be smaller. Upon the passage of electric current, the biomolecules will
pass through membrane (b) and collect in Dialysis Chamber (2) while smaller molecules will connue to
pass through membrane (b) and (a). This is a fast and eecve method for selecve ltraon, and for
concentrang small samples.
Steps
1. Select the proper size Dialyzer Chamber to use as much of the available chamber volume as possible
(minimize air gaps).
2. Assemble Dialyzer Unit by placing one membrane (a) on the plaorm of the Dialyzer Chamber (2)
and hand-ghtening an End Cap.
3. Add buer to the Dialyzer Chamber.
4. Place your next membrane (b) on the other side of the Dialyzer Chamber and assemble the Union to
create a combined large compartment.
5. Assemble second Dialyzer Chamber (1) to the Union and add your sample to the combined
large compartment.
6. Place your next membrane (c) on the other side of the combined chamber and assemble End Cap.
7. Gently push the assemblage of chambers through the ElectroPrep tank gasket to secure the dialysis
unit in place.
8. Fill the Electroprep tank with electroeluon buer and ensure the Dialysis Chambers are covered,
but the uid does not ow over the paron. Chambers and caps must be completely immersed
in buer so that no air bubbles are present. Keeping all parts submersed in buer prevents the
introducon of air bubbles into the Dialyzer Chambers.
9. Assemble the lid with cables and connect to your power supply. Use current and voltage sengs as
required for the sample and buer (5 to 10 mA suggested as a starng point). Eluon me can be
calculated by measuring the me required for the biomolecule to migrate 1 cm during gel electrophoresis.




Rapid and Selecve
Electro-Filtraon or
Concentraon
ElectroPrep Dialyzer
Open End Cap
Membrane
Union
Key

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ElectroPrep System
Publicaon 9511-073 REV-1.0
ElectroPrep Protocols
Electroeluon and Electrofraconaon of DNA, Proteins or Other
Biomolecules from Gel Pieces (Variaon of Conguraon #2)
Applicaons
• Eluon of DNA, proteins, or other biomolecules from one or more gel pieces. Oponally, electrofracon
of eluted biomolecules by size may be done at the same me.
Using the ElectroPrep system in this Conguraon #2 variant, eluon of DNA, proteins, or any other biomolecules
from a gel slice/plug can be achieved quickly and easily with excellent recovery. Using a Union, Chambers can be
joined in any combinaon necessary to accommodate the required gel volume. Samples can be concentrated if
desired, by choosing a receiving Chamber of suitable smaller volume. The MWCO of the membranes (a and b) can
also be chosen to achieve very selecve ltraon or size fraconaon during the electroeluon process.
Steps
1. Choose desired eluon and sample Dialyzer Chambers, Union and Membranes.
2. Assemble Dialyzer Unit (beginning with Dialyzer Chamber that will be the eluon sample collecon
chamber). Place Membrane (a), lower MWCO than your biomolecule, on the membrane plaorm of the
Dialyzer chamber (2) and add an Open End Cap (hand ghten).
3. Add buer to the eluon sample collecon Dialyzer Chamber.
4. Place membrane (b), MWCO larger than your biomolecule, into the eluon sample collecon Dialyzer
Chamber. Add Union onto other end.
5. Assemble second Dialyzer Chamber (1) to the Union to create a combined large compartment, and add gel
slice(s) and buer to the combined large compartment.
6. Place your next Membrane (c), lower MWCO than your biomolecule on the other side of the combined
large compartment and assemble End Cap.
7. Gently push the assemblage of chambers through the ElectroPrep tank gasket to secure the dialysis unit in place.
8. Fill the Electroprep tank with electroeluon buer and ensure the Dialysis Chambers are covered, but the
uid does not ow over the paron. Chambers and caps must be completely immersed in buer so that
no air bubbles are present. Keeping all parts submersed in buer prevents the introducon of air bubbles
into the Dialyzer Chambers.
9. Assemble the lid with cables and connect to your power supply. Set current and voltage sengs as
required for the sample, gel, and buer (15 mA suggested as a starng point). Eluon me can be
calculated by measuring the me required for the biomolecule to migrate 1 cm during gel electrophoresis.



Electro-Elution of DNA,
Proteins or Other Biomolecules
from Gel Pieces
ElectroPrep Dialyzer
Open End Cap
Membrane
Union
Gel
Key

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ElectroPrep System
Publicaon 9511-073 REV-1.0
Specifications
TECHNICAL SPECIFICATIONS
Maximum Power 50 W
Maximum Voltage 1000 V
Maximum Current 500 mA
Maximum Liquid Temperature 45°C
Unit Dimensions (L x H x W) 9.8 x 5.2 x 5.5 in (25 x 13.2 x 13.14 cm)
Weight 1.9 lb (0.86 kg)
Maximum Buer Volume 1000 ml
Power Output Connectors
(Diameter)
Shrouded, 4 mm
Installaon Category II
ENVIRONMENTAL CONDITIONS
Locaon Indoor use only
Operaon, Ambient
Temperature
5 to 40°C
Operaon, Maximum Relave
Humidity
80% (non-condensing)
Operaon, Altude 2000 m
Shipping and Transportaon,
Ambient Temperature
-20 to 60°C
Shipping and Transportaon,
Maximum Relave Humidity
95% (non-condensing)
Polluon Degree 2*
* The apparatus is rated POLLUTION DEGREE 2 in accordance with IEC 60664-1.
POLLUTION DEGREE 2, states that: “Normally only non-conducve polluon occurs.
Occasionally, however, a temporary conducvity caused by condensaon must
be expected”.

17
ElectroPrep System
Publicaon 9511-073 REV-1.0
General Care & Maintenance
Tank
• Disconnect leads from power supply before servicing unit.
• To remove the safety lid, push thumbs down on the plasc lugs and li the lid vercally
with your ngers.
• Before use, clean and dry the apparatus with DISTILLED WATER ONLY.
IMPORTANT: Acrylic plasc is NOT resistant to aromac or halogenated hydrocarbons,
ketones, esters, alcohols (over 25%) and acids (over 25%); they will cause crazing and
cracking of UV transparent plasc and should NOT be used for cleaning. DO NOT use
abrasive creams or scourers. Dry components with clean ssues prior to use.
• Before use, check the unit for any leaks at the bonded joints. Place the unit on a sheet
of dry ssue and then ll with DISTILLED WATER ONLY to the maximum ll line. Any
leakage will be seen on the ssue paper. If any leakage is seen DO NOT ATTEMPT TO
REPAIR OR USE THE APPARATUS, but nofy Harvard Apparatus immediately.
• The replacement planum electrodes are parally shrouded for protecon. However,
when cleaning the main tank DO NOT use cleaning brushes in the electrode area.
Usually a thorough rinse with dislled water is all that is required.
• Ensure that the connectors are clean and dry before usage or storage.
PTFE Chambers
• Clean by rinsing with deionized (DI) water prior to each use.
• Take care to not damage the threads.
• For cases where contaminaon is severe, low concentraon detergents may be used,
followed by rinsing thoroughly with deionized (DI) water.
Membranes
• Prior to use, rinse with deionized (DI) water and treat with your buer soluon.
• Discard membranes aer use. Do not reuse.

18
ElectroPrep System
Publicaon 9511-073 REV-1.0
Ordering Information
Descripon Item #
ElectroPrep Hardware
ElectroPrep Tank, with lid, gasket, 4 mm red and black
connector cables
74-1196
ElectroPrep Replacement Connector Cables, 4 mm, red and
black (1 each)
74-1197
Power Supply for Electroprep, 300 V, 500 mAmp, 90W 74-1103
Replacement Gasket, Qty. of 3 74-1113
Dialysis Chambers, Qty. of 2, 15/16” Inner Diameter
50 µl Chamber Volume 7411-502D
100 µl Chamber Volume 7411-1002D
250 µl Chamber Volume 7411-2502D
500 µl Chamber Volume 7411-5002D
1000 µl Chamber Volume 7411-10002D
1500 µl Chamber Volume 7411-15002D
Link Chambers, Qty. of 2
50 µl Chamber Volume, (1) 11/16" dia Primary link and (1)
7/16'' dia Secondary link
7411-502L
100 µl Chamber Volume, (1) 11/16" dia Primary link and
(1) 7/16'' dia Secondary link
7411-1002L
250 µl Chamber Volume, (2) 11/16" dia Primary links 7411-2502L
500 µl Chamber Volume, (2) 11/16" dia Primary links 7411-5002L
1000 µl Chamber Volume, (2) 11/16" dia Primary links 7411-10002L
1500 µl Chamber Volume, (2) 11/16" dia Primary links 7411-15002L
Union (Dialysis Chamber Connector), Qty. of 2, 15/16” Diameter
(1) 600 µl and (1) 3500 µl to join Dialysis Chambers 74-1194
ElectroPrep Membranes for All Dialysis Chambers (50 to 1500 µl, 15/16” Diameter)
Type and MWCO
Regenerated Cellulose
1 kDa MWCO 7410-RC1K
2 kDa MWCO 7410-RC2K
3.5 kDa MWCO 7410-RC3.5K
10 kDa MWCO 7410-RC10K

19
ElectroPrep System
Publicaon 9511-073 REV-1.0
Ordering Information
Descripon Item #
25 kDa MWCO 7410-RC25K
50 kDa MWCO 7410-RC50K
Cellulose Acetate
500 Da MWCO 7410-CA500
1 kDa MWCO 7410-CA1K
2 kDa MWCO 7410-CA2K
5 kDa MWCO 7410-CA5K
10 kDa MWCO 7410-CA10K
25 kDa MWCO 7410-CA25K
50 kDa MWCO 7410-CA50K
100 kDa MWCO 7410-CA100K
300 kDa MWCO 7410-CA300K
Polycarbonate
0.01 µm Pore Size 7410-PC01
0.05 µm Pore Size 7410-PC05
0.10 µm Pore Size 7410-PC10
0.60 µm Pore Size 7410-PC60
ElectroPrep Membranes for Primary Link Chambers (50 to 250 µl, 11/16” Diameter)
Type and MWCO
Regenerated Cellulose
1 kDa MWCO 7416-RC1K
2 kDa MWCO 7416-RC2K
3.5 kDa MWCO 7416-RC3.5K
10 kDa MWCO 7416-RC10K
25 kDa MWCO 7416-RC25K
50 kDa MWCO 7416-RC50K
Cellulose Acetate
500 Da MWCO 7416-CA500
1 kDa MWCO 7416-CA1K
2 kDa MWCO 7416-CA2K
5 kDa MWCO 7416-CA5K