Hettich MIKRO 200 User manual

Rev. 02 / 12.2022 Andreas Hettich GmbH & Co. KG AB2400EN_SA
MIKRO 200
MIKRO 200 R
Operating Instructions.................................................... 4

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Fig. 1
Fig. 2 MIKRO 200
Fig. 3 MIKRO 200 R

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Andreas Hettich GmbH & Co. KG
Föhrenstraße 12, D-78532 Tuttlingen / Germany
Phone [phone] / 705-0
Fax [phone]
[e-mail], ser[email protected]
www.hettichlab.com
Single Registration Number: DE-MF-000010680
© 2003 by Andreas Hettich GmbH & Co. KG
All rights reserved. No part of this publication may be reproduced without the prior written permission of the copyright
owner.
Änderungen vorbehalten! , Modifications reserved! , Sous réserve de modifications ! , Con riserva di modifiche!
AB2400EN_SA / Rev. 02

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Contents
1 Use according to specification ................................................................................................................................6
2 Remaining risks ......................................................................................................................................................6
3 Technical specifications..........................................................................................................................................6
4 Notes on safety.......................................................................................................................................................7
5 Transport and storage ............................................................................................................................................9
5.1 Transport .........................................................................................................................................................9
5.2 Storage............................................................................................................................................................9
6 Symbol meanings .................................................................................................................................................10
7 Delivery checklist..................................................................................................................................................11
8 Unpacking the centrifuge ......................................................................................................................................11
9 Initial operation .....................................................................................................................................................11
10 Opening and closing the lid ...........................................................................................................................12
10.1 Opening the lid ..............................................................................................................................................12
10.2 Closing the lid................................................................................................................................................12
11 Installation and removal of the rotor ..............................................................................................................13
12 Loading the rotor ...........................................................................................................................................13
13 Closing biosafety systems .............................................................................................................................14
14 Control and display elements ........................................................................................................................14
14.1 Control knob ..................................................................................................................................................14
14.2 Control panel pushbuttons (keys)..................................................................................................................14
14.3 Adjustment possibilities .................................................................................................................................15
15 Programming.................................................................................................................................................15
15.1 Programme input/alteration ...........................................................................................................................15
15.2 Programme recall ..........................................................................................................................................15
16 Centrifugation ................................................................................................................................................16
16.1 Centrifugation with pre-set time.....................................................................................................................16
16.2 Continuous run ..............................................................................................................................................16
16.3 Short-term centrifugation ...............................................................................................................................16
17 Emergency Stop............................................................................................................................................16
18 Acoustic Signal..............................................................................................................................................17
19 Recall hours of operation...............................................................................................................................17
20 Cooling (only in centrifuges with cooling) ......................................................................................................17
20.1 Standby-cooling.............................................................................................................................................17
20.2 Pre-cooling the rotor ......................................................................................................................................17
21 Relative centrifugal force (RCF) ....................................................................................................................18
22 Centrifugation of materials or mixtures of materials with a density higher than 1.2 kg/dm3...........................18
23 Emergency release........................................................................................................................................18
24 Maintenance and servicing ............................................................................................................................19
24.1 Centrifuge (housing, lid and centrifuging chamber) .......................................................................................19
24.1.1 Surface cleaning and care ..................................................................................................................19
24.1.2 Surface disinfection ............................................................................................................................19
24.1.3 Removal of radioactive contaminants .................................................................................................19
24.2 Rotors and Attachments................................................................................................................................20

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24.2.1 Cleaning and care...............................................................................................................................20
24.2.2 Disinfection .........................................................................................................................................20
24.2.3 Removal of radioactive contaminants .................................................................................................20
24.2.4 Rotors and accessories with limited service lives ...............................................................................20
24.3 Autoclaving ....................................................................................................................................................21
24.4 Centrifuge containers.....................................................................................................................................21
25 Faults.............................................................................................................................................................22
26 Change mains input fuses .............................................................................................................................23
27 Returning Devices .........................................................................................................................................23
28 Disposal.........................................................................................................................................................24
29 Anhang / Appendix ........................................................................................................................................25
29.1 Rotoren und Zubehör / Rotors and accessories ............................................................................................25

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1 Use according to specification
The centrifuge MIKRO 200 / MIKRO 200 R is an in vitro diagnostic medical device according to the ln Vitro
Diagnostic Medical Devices Regulation (EU) 2017/746.
The device is used for centrifuging and enriching sample material of human origin for subsequent further processing
for diagnostic purposes. The user can set each of the variable physical parameters within the limits set by the device.
The centrifuge may only be used by qualified personnel in closed laboratories. The centrifuge is only intended for the
use referred to above. Intended use also includes observing all instructions in the Operating Manual and compliance
with the required inspection and maintenance work.
Any other use or use beyond this is considered improper. Andreas Hettich GmbH & Co. KG shall not be liable for any
damage arising from this.
2 Remaining risks
The device is built according to the state-of-the-art and the recognized safety regulations. If used and handled
improperly, there could be life-threatening danger to the user or third parties, or the device could be impaired or there
could be other property damage. The device is only to be used for its intended purpose and only when it is in safe
working condition.
Malfunctions which could affect safety must be corrected immediately.
3 Technical specifications
Manufacturer Andreas Hettich GmbH & Co. KG
D-78532 Tuttlingen
Model MIKRO 200 MIKRO 200 R
Basic-UDI-DI 4050674010010PZ
Type 2400 2400-01 2405 2405-07 2405-01
Mains voltage (r10%) 200-240 V 1a100-127 V 1a200-240 V 1a200-240 V 1a100-127 V 1a
Mains frequency 50 – 60 Hz 50 – 60 Hz 50 Hz 60 Hz 50 – 60 Hz
Connected load 240 VA 270 VA 450 VA 450 VA 630 VA
Current consumption 1.2 A 2.7 A 1.9 A 2.0 A 5.0 A
Cooling medium ---- R 134a
Max. capacity 24 x 2.0 ml
Allowed density 1.2 kg/dm3
Speed (RPM) 15000
Force (RCF) 21382
Kinetic energy 5800 Nm
Obligatory inspection
(DGUV Regel 100 – 500)
no
Ambient conditions (EN / IEC 61010-1)
 Set-up site Indoors only
 Altitude Up to 2000 m above sea level
 Ambient temperature 2°C to 40°C 5°C to 35°C
 Humidity Maximum relative humidity 80% for temperatures up to 31°C, linearly
decreasing to 50% relative humidity at 40°C.
 Excess-voltage category
(IEC 60364-4-443) ,,
 Pollution degree 2
Device protection class ,
Not suitable for use in explosion-endangered areas.
EMC
 Emitted interference,
Interference immunity
EN / IEC
61326-1,
Class B
FCC Class B EN / IEC 61326-1,
Class B
FCC Class B
Noise level (dependent on rotor) d58 dB(A) d53 dB(A) d54 dB(A)
Dimensions
 Width 275 mm 281 mm
 Depth 344 mm 553 mm
 Height 260 mm 260 mm
Weight approx. 11.5 kg approx. 28 kg

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4 Notes on safety
No claim of warranty will be considered by the manufacturer unless ALL instructions in this manual
have been followed.
Reports of serious incidents involving the device
Report any serious incidents involving the device to the manufacturer and, if necessary, to the competent
authority.
x The centrifuge should be installed on a good, stable base.
x Before using the centrifuge absolutely check the rotor for firm placement.
x When the centrifuge is running, according to EN / IEC 61010-2-020, no persons, dangerous
substances or objects may be within the safety margin of 300 mm around the centrifuge.
x Rotors, suspensions and accessories that possess traces of corrosion or mechanical damage or
if their term of use has expired may not be used any longer.
x The centrifuge may no longer be put into operation when the centrifuging chamber has safety-
related damages.
x With swing-out rotors the trunnions must be regularly lubricated (Hettich Lubricating Grease No.
4051) in order to ensure consistent swinging out of the hangers.
x For centrifuges without temperature control, when the room temperature is increased and/or if the
device is frequently used, the centrifuging chamber could be heated up. Therefore, it can't be
ruled out that the sample material might be changed due to the temperature.
x Before the initial operation of your centrifuge you should read and pay attention to the operating
instructions. Only personnel that has read and understood the operating instructions are allowed to
operate the device.
x Along with the operating instructions and the legal regulations on accident prevention, you should also follow the
recognised professional regulations for working in a safe and professional manner. These operating instructions
should be read in conjunction with any other instructions concerning accident prevention and environmental
protection based on the national regulations of the country where the device is to be used.
x This centrifuge is a state-of-the-art piece of equipment which is extremely safe to operate. However, it can lead to
danger for users or others if used by untrained staff, in an inappropriate way or for a purpose other than that it
was designed for.
x The centrifuge must not be moved or knocked during operation.
x In case of fault or emergency release, never touch the rotor before it has stopped turning.
x To avoid damage due to condensate, when changing from a cold to a warm room the centrifuge must either heat
up for at least 3 hours in the warm room before being connected to the mains, or run hot for 30 minutes in the
cold room.
x Only the rotors and accessories approved by the manufacturer for this device may be used (see chapter
"Anhang/Appendix, Rotoren und Zubehör/Rotors and accessories"). Before centrifuge vessels are used which are
not listed in the chapter "Appendix, Rotors and accessories", the user must make sure they can be used by
asking the manufacturer.
x The centrifuge rotor may only be loaded in accordance with the chapter "Loading the rotor".
x When centrifuging with maxim revolutions per minute the density of the materials or the material mixtures may not
exceed 1.2 kg/dm3.
x The centrifuge may only be operated when the balance is within the bounds of acceptability.
x The centrifuge may not be operated in explosion-endangered areas.
x The centrifuge must not be used with:
 inflammable or explosive materials
 materials that react with one another producing a lot of energy.

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x When centrifuging hazardous substances or mixtures, which are toxic, radioactive or contaminated with
pathogenic microorganisms, suitable measures must be taken by the user.
Fundamentally, centrifuge containers with special screw closures must be used for hazardous substances. For
materials of risk groups 3 and 4, in addition to sealable centrifuge containers, a bio-safety system must be used
(see the "Laboratory Bio-safety Manual" from the World Health Organization).
In a bio-safety system, a bio-seal (sealing ring) prevents droplets and aerosols from escaping.
If the hanger of a bio-safety system is used without the lid, the sealing ring must be removed from the hanger to
prevent damage to the sealing ring during the centrifugation run.
Damaged bio-safety systems are no longer microbiologically sealed.
If a bio-safety system is not used, a centrifuge is not microbiologically sealed for the purposes of the standard EN
/ IEC 61010-2-020.
When closing a bio-safety system, follow the instructions in the chapter "Closing biosafety systems".
For the available bio-safety systems, see the chapter "Appendix, Rotors and accessories". If in doubt, you can
get the information you need from the manufacturer.
x The centrifuge must not be operated with highly corrosive substances which could impair the mechanical integrity
of rotors, hangers and accessories.
x Repairs must only be carried out by personnel authorised to do so by the manufacturer.
x Only original spare parts and original accessories licensed by the Andreas Hettich GmbH & Co. KG company are
allowed to be utilised.
x The following safety regulations apply:
EN / IEC 61010-1 and EN / IEC 61010-2-020 as well as their national deviations.
x The safe operation and reliability of the centrifuge can only be guaranteed if:
 the centrifuge is operated in accordance with the operating instructions,
 the electrical installation on the site where the centrifuge is installed conforms to the demands of EN / IEC
stipulations.
x Meeting the country-specific requirements concerning occupational safety with regard to the use of laboratory
centrifuges at the workplaces provided for this purpose by the user is the responsibility of the user.

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5 Transport and storage
5.1 Transport
Before transporting the device, the transport securing device must be installed.
When the device and accessories are transported, the following ambient conditions must be complied with:
x Ambient temperature: -20°C to +60°C
x Relative humidity: 10% to 80%, non-condensing
5.2 Storage
The device and the accessories may only be stored in closed and dry rooms.
When the device and accessories are stored, the following ambient conditions must be complied with:
x Ambient temperature: -20°C to +60°C
x Relative humidity: 10% to 80%, non-condensing

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6 Symbol meanings
Symbol on the device:
Attention, general hazard area.
Symbol on the device:
Observe operating instructions.
This symbol indicates that the user must observe the operating instructions provided.
Symbol in this document:
Attention, general hazard area.
This symbol refers to safety relevant warnings and indicates possibly dangerous situations.
The non-adherence to these warnings can lead to material damage and injury to personal.
Symbol on the device and in this document:
Beware of biohazard.
Symbol in this document:
This symbol refers to important circumstances.
Symbol on the device and in this document:
Symbol for the separate collection of electric and electronic devices according to the guideline
2012/19/EU.
Applies in the countries of the European Union, as well as in Norway and Switzerland.
Symbol on the shipping carton label:
This way up.
Symbol on the shipping carton label:
The shipping packaging must be transported and
handled within the indicated humidity range (10% - 80%).
Symbol on the shipping carton label:
The shipping packaging must be stored, transported and
handled within the temperature range shown (-20°C - +60°C).
Symbol on the shipping carton label:
The shipping packaging must be kept away from rain and
kept in a dry environment.
Symbol on the shipping carton label:
Fragile, handle with care.
Symbol on the shipping carton label:
Stack limit. Maximum number of identical packages which may be stacked on the bottom package,
whereby “n“ stands for the number of permissible packages. The bottom package is not included in “n”.

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7 Delivery checklist
1 Connecting cable
2 Fuses
1 Hex. pin driver 2,5 mm
1 Hex. pin driver 5 mm
1 Operating instructions
1 Notes on moving the equipment safely
The rotor(s) and associated accessories are included in the delivery in the quantity ordered.
8 Unpacking the centrifuge
x Lift the carton upward and remove the padding.
x Do not lift by the front panel.
Observe the weight of the centrifuge, refer to chapter "Technical specifications".
Lift the centrifuge on both sides with an appropriate number of helpers and place it on the laboratory table.
9 Initial operation
x Remove the transportation safety device from the bottom of the housing, see sheet ”Transportation safety
device"
x Position the centrifuge in a stable and level manner in a suitable place. During set-up, the required safety
margin of 300 mm around the centrifuge is to be kept according to EN / IEC 61010-2-020.
When the centrifuge is running, according to EN / IEC 61010-2-020, no persons, dangerous
substances or objects may be within the safety margin of 300 mm around the centrifuge.
x Ventilation openings may not be blocked.
A distance of 300 mm must be maintained from the ventilation slots and openings of the centrifuge.
x Check whether the mains voltage tallies with the statement on the type plate.
x Connect the centrifuge with the power cord to a standard mains socket. For connection ratings refer to Chapter
"Technical specification".
x Turn on the mains switch. Switch position ",".
The machine type and program version will be displayed and the LEDs light up. After 8 seconds
OPEN OEFFNEN will be displayed on centrifuges with cooling and the left LED in the key will
flash. On centrifuges without cooling the cover will open automatically and the last used centrifuge data will be
displayed.
x Open the cover on centrifuges with cooling.
The last used centrifuge data will be displayed.
x Remove the transportation safety device in the centrifuge, see sheet ”Transportation safety device”.

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10 Opening and closing the lid
10.1 Opening the lid
The lid can only be opened when the centrifuge is switched on and the rotor is at rest. If it cannot be
opened under these circumstances, see the section on “Emergency release”.
x Press the button The lid unlocks via the motor and the left LED in the pushbutton
extinguishes.
The cover opens automatically approx. 45°.
This opening angle can be adjusted, if needed.
x Turn the adjusting screw (A) with the hex head wrench included in delivery.
Turning clockwise: Cover is opened less.
Turning counterclockwise: Cover is opened wider.
10.2 Closing the lid
Do not put your fingers between lid and housing.
Do not bang the lid shut.
If the left LED in the button flashes, press the button so that the motor-driven lid
lock goes into the basic position (opened).
x Place the lid and lightly press down the front edge of the lid. The locking action is effected by motor. The left LED
in the button lights up.

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11 Installation and removal of the rotor
x Clean the motor shaft (C) and the rotor drilling (A), and lightly grease the motor shaft
afterwards. Dirt particles between the motor shaft and the rotor hinder a perfect seating
of the rotor and cause an irregular operation.
x Place the rotor vertically on the motor shaft. The motor shaft dog (D) has to fit in the
rotor slot (B). The alignment of the groove is labelled on the rotor.
x Tighten the rotor tension nut with the supplied wrench by turning in a clockwise
direction.
x Check the rotor for firm seating.
To ensure a tight fit of the rotor, the nut of the rotor must be hand-
tightened.
x Loosening the rotor: Loosen the tension nut by turning in a counter clockwise direction,
and turning until the working point for lifting. After passing the working point for lifting
the rotor is loosened from the motor shaft cone. Turn the tension nut until the rotor is
able to be lifted from the motor shaft.
12 Loading the rotor
Standard centrifuge containers of glass will not stand RCF values exceeding 4000 (DIN 58970, pg. 2).
x Check the rotor for firm seating.
x The rotors must be loaded symmetrically. The centrifuge containers have to be distributed evenly on all rotor
positions. For authorised combinations see Chapter "Anhang/Appendix, Rotoren und Zubehör/Rotors and
accessories".
Example:
Rotor is evenly loaded Not permitted!
Rotor is not evenly loaded
x The centrifuge containers may only be filled outside of the centrifuge.
x The maximum filling quantity for the centrifuge containers specified by the manufacturer must not be exceeded.
The centrifuging vessels may only be filled so
far that no fluid can be expelled from them
while the centrifuge is running.
Fluid
Centrifugal force
x When loading the rotor, no liquid may enter the rotor or the centrifuging chamber.
x In order to maintain the weight differences within the centrifuge container as marginal as possible, a consistent
fill level in the containers is to be heeded.
x The weight of the permissible filling quantity is specified on each rotor. This weight may not be exceeded.

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13 Closing biosafety systems
In order to guarantee that it is sealed, the lid to the biosafety system has to be firmly closed.
In order to prevent the packing ring from twisting when opening and closing the cover, the packing ring must
be lightly rubbed with a rubber care product.
For further details of available biosafety systems see chapter "Anhang/Appendix, Rotoren und Zubehör/Rotors
and accessories". If in doubt, you should obtain relevant information from the manufacturer.
Lid with screw top, without hole in turning handle
x Place the lid centred on the rotor.
x Tightly seal the lid by hand by turning the turning handle clockwise.
14 Control and display elements
See figure on page 2.
Fig. 2, Fig. 3: Display and control panel
14.1 Control knob
For setting the individual parameters.
Turning anticlockwise reduces the value. Turning clockwise increases the value.
14.2 Control panel pushbuttons (keys)
x Selection control key for selection of specific parameter.
The subsequent parameter is selected by every further keystroke.
x Start centrifugation run. The LED in the button lights up during the centrifugation run as long as the
rotor is turning.
x Short-term centrifugation.
The centrifugation run is effected as long as the button is held down. The LED in the button lights up
during the centrifugation run as long as the rotor is turning.
x Store inputs and changes.
x End centrifugation run.
The rotor runs down with a pre-selected brake step. The right-hand LED in the button lights up until the
rotor is stationary. Once the rotor is stationary the left-hand LED flashes in the button. Pressing the
button twice triggers the EMERGENCY STOP.
x Unlock the lid.
The left-hand LED in the button goes out.
x Leave the parameter input.
x Switch between RPM and RCF display.
RCF values are displayed in > <.
x Start pre-cooling.
The pre-cooling speed is settable. It is pre-adjusted to 10000 RPM.

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14.3 Adjustment possibilities
PROG RCL Program position of the called-up program.
t/min Running time. Settable from 0 - 99 min, in 1 min increments.
t/sec Running time. Settable from 0 - 59 s, in 1 second increments.
Continuous run "f". Set parameter t/min and t/sec to zero.
RPM Revolutions per minute. A numerical value from 500 RPM up to the maximum speed of the rotor can be
set. Maximum speed of the rotor, see Chapter "Anhang/Appendix, Rotoren und Zubehör/Rotors and
accessories". Settable in increments of 10.
RAD/mm Centrifugation radius. Input in mm. For centrifugation radius see Chapter "Anhang/Appendix, Rotoren
und Zubehör/Rotors and accessories". The input of the radius is only possible if the RCF display
(> RCF <) is selected.
RCF Relative centrifugal force. A numerical value can be set, which gives a speed between 500 RPM and the
maximum speed of the rotor. Adjustable up to 100 in intervals of 1, and from 100 in intervals of 10. The
RCF value is automatically rounded up or rounded down with regard to the RPM interval. The input of
the RCF is only possible if the RCF display (> RCF <) is selected.
Starting steps 1 - 9. Step 9 = shortest starting time, Step 1 = longest starting time.
Brake steps 1 - 9. Step 9 = shortest run-down time, Step 1 = longest run-down time.
T/°C Temperature Set Point (only in centrifuges with cooling). Adjustable from -10°C to +40°C, in 1°C
intervals. The lowest obtainable temperature depends on the rotor (see Chapter "Anhang/Appendix,
Rotoren und Zubehör/Rotors and accessories").
PROG STO Program position on which the program is stored. 4 programs can be stored (program positions 1 - 2 – 3
- 4).
15 Programming
15.1 Programme input/alteration
If no key is pressed for 8 seconds long after the selection or during the input of parameters, the previous
values will be shown in the display. The input of parameter then has to be executed again.
x Select the RPM or RCF display with the key . RCF values are displayed in > <.
x Select the desired parameters using the button and set using the knob .
In order to set continuous operation, the parameters t/min and t/sec must be set to zero with the knob.
Continual running is represented in the display by the following symbol, "f".
x The parameter PROG STO can be selected using the button and the desired program position set using
the knob .
x Press the button in order to store the setting on the desired program position.  ok  is
displayed briefly as confirmation.
The previous data in the program position is overwritten during saving.
15.2 Programme recall
x Select the parameter PROG RCL using the button and set the desired program position using the knob
.
x Press the button . The centrifugation data of the selected program position is displayed.
x The parameters can be checked by pressing the button .
To leave the parameter display press the button or press no button for a period of 8 seconds.

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16 Centrifugation
When the centrifuge is running, according to EN / IEC 61010-2-020, no persons, dangerous substances or
objects may be within the safety margin of 300 mm around the centrifuge.
If the permissible weight difference within the rotor loading has been exceeded, the drive shuts down during
the start-up, the unbalance display lights up, and IMBALANCE is displayed.
A centrifugation run can be stopped at any time by pushing the key .
All parameters can be selected and altered during the centrifugation run (see Chapter “Programming”).
You can switch-over at any time between the RPM and RCF display with the key . The input of the
centrifugation radius is necessary if you are working with the RCF display.
If OPEN OEFFNEN is displayed, a further operation of the centrifuge is only possible after opening the lid
once.
x Turn on the mains switch. Switch position ,.
x Load the rotor and close the centrifuge lid.
16.1 Centrifugation with pre-set time
x Adjusting time or recall a programme with pre-set time (see Chapter "Programming").
x Press the key . The LED in the button lights up for as long as the rotor turns.
x After expiration of the time or with truncation of the centrifugation run by pushing the key , the run-
down is effected with the selected brake step. The brake step is displayed.
During the centrifugation run, the rotor speed or the RCF value, the temperature in the centrifuging chamber (only for
centrifuge with cooling), and the remaining time are displayed. After the centrifuge has ended its run and the rotor
has come to a halt, the lid opens automatically on centrifuges without cooling.
16.2 Continuous run
x Adjusting the symbol for recall a continuous run programme (see Chapter "Programming").
x Press the key . The LED in the button lights up for as long as the rotor turns. The
time metering begins at 00:00.
x Press the key in order to stop the centrifugation run. The run-down is effected with the selected
brake step. The brake step is displayed.
During the centrifugation run, the rotor speed or the RCF value, the temperature in the centrifuging chamber (only for
centrifuge with cooling), and the elapsed time are displayed. After the centrifuge has ended its run and the rotor has
come to a halt, the lid opens automatically on centrifuges without cooling.
16.3 Short-term centrifugation
x Hold down the key. The LED in the button lights up for as long as the rotor turns. The time
metering begins at 00:00.
x Let go of the key again in order to stop the centrifugation run. The run-down is effected with the
selected brake step. The brake step is displayed.
During the centrifugation run, the rotor speed or the RCF value, the temperature in the centrifuging chamber (only for
centrifuge with cooling), and the elapsed time are displayed. After the centrifuge has ended its run and the rotor has
come to a halt, the lid opens automatically on centrifuges without cooling.
17 Emergency Stop
x Press the key twice.
With Emergency Stop the run-down is effected with brake step 9 (shortest run-down time). Brake step 9 is displayed.

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18 Acoustic Signal
The acoustic signal sounds:
x Upon the appearance of a disturbance in 2 second intervals.
x After completion of a centrifugation run and rotor standstill in 30 second intervals (only in centrifuges with
cooling).
The acoustic signal is stopped by opening the lid or pressing any key.
The signal after completion of the centrifugation run can be activated or deactivated in the following manner, if the
rotor is at standstill:
x Hold down the key for 8 seconds.
After 8 seconds, SOUND / BELL appears in the display.
x Set using the knob OFF or ON.
x Press the key in order to store the setting.
As confirmation,  ok  will be displayed for a short period.
19 Recall hours of operation
Recall hours of operation is only possible during rotor standstill.
x Hold down the key for 8 seconds.
After 8 seconds, SOUND / BELL appears in the display.
x Press the key once again.
The centrifuge’s hours of operation (CONTROL: ) are displayed.
x Press the key to exit the hours of operation recall.
20 Cooling (only in centrifuges with cooling)
The temperature set-point can be adjusted from -10°C to +40°C. The lowest obtainable temperature is dependent on
the rotor (see Chapter "Anhang/Appendix, Rotoren und Zubehör/Rotors and accessories").
20.1 Standby-cooling
With rotor standstill and closed lid the centrifugal chamber is cooled to the pre-selected temperature. The
temperature set-point is shown in the display.
Standby cooling will be subject to a timed delay after a centrifuge run and the display will show OPEN
OEFFNEN. The delay time can be pre-set in 1minute steps from 1 to 5 minutes. It is pre-set to 1 minute.
With the rotor standing still and the cover open the delay time can be set as follows:
x Hold down the key for 8 seconds.
After 8 seconds, t/min = X appears in the display.
x Use the rotary button to set the delay time.
x Press the key in order to store the setting.
As confirmation,  ok  will be displayed for a short period.
To leave the delay time display press the key or do not press any key for a period of 8 seconds.
20.2 Pre-cooling the rotor
x Press the key . The LED in the button lights up for as long as the rotor turns.
x Press the button to end the pre-cooling. The run-down is effected with the selected brake step. The
brake step is displayed.
During the centrifugation run, the rotor speed or the RCF value, the temperature in the centrifuging chamber, and the
elapsed time are displayed.
The pre-cooling speed can be adjusted in decadic steps from 500 RPM to the max RPM of the rotor. It is pre-
adjusted to 10000 RPM.
When the rotor is stationary and the lid open the pre-cooling speed can be set in the following manner:
x Hold down the key for 8 seconds.
After 8 seconds, t/min = X appears in the display.
x Press the key once again.
The set pre-cooling RPM - RPM = XXXX will be displayed.
x Set the desired pre-cooling speed using the knob .
x Press the key in order to store the setting.
As confirmation,  ok  will be displayed for a short period.
To leave the pre-cooling RPM display press the key or do not press any key for a period of 8 seconds.

18/27 Rev. 02 / 12.2022 AB2400EN_SA
21 Relative centrifugal force (RCF)
The relative centrifugal force (RCF) is given as a multiple of the acceleration of gravity (g). It is a unit-free value and
serves to compare the separation and sedimentation performance.
These values are calculated using the formula below:
1,118r
2
1000
RPM
RCF uu ¸
¹
·
¨
©
§1000
1,118r
RCF
RPM u
u
RCF = relative centrifugal force
RPM = rotational speed (revolutions per minute)
r = centrifugal radius in mm = distance from the centre of the turning axis to the bottom of the centrifuge
container. For more on the centrifugal radius see the chapter
“Anhang/Appendix,Rotoren und Zubehör/Rotors and accessories”.
The relative centrifugal force (RCF) stands in relation to the revolutions per minute and the centrifugal
radius.
22 Centrifugation of materials or mixtures of materials with a density higher than 1.2 kg/dm3
When centrifuging with maxim revolutions per minute the density of the materials or the material mixtures may not
exceed 1.2 kg/dm3.
The speed must be reduced for materials or mixtures of materials with a higher density.
The permissible speed can be calculated using the following formula:
[RPM]speedmaximumx
[kg/dm³]densityGreater
1.2
=)(nspeedReduced red
e.g.: maximum speed RPM 4000, density 1.6 kg/dm3
RPM3464=RPM4000x
kg/dm³1.6
kg/dm³1.2
=nred
In the exceptional case that the maximum loading indicated on the hanger is exceeded, the speed must also be
reduced.
The permissible speed can be calculated using the following formula:
[RPM]speedmaximumx
[g]loadactual
[g]loadmaximum
=)(nspeedReduced red
e.g.: maximum speed RPM 4000, maximum load 300 g, actual load 350 g
RPM3703=RPM4000x
g350
g300
=nred
If in doubt you should obtain clarification from the manufacturer.
23 Emergency release
During a power failure the lid cannot be unlocked by motor. An emergency release has to be executed by hand.
For emergency release disconnect the centrifuge from the mains.
Open the lid only during rotor standstill.
See figure on page 2.
x Switch off the mains switch (switch position "0").
x Look through the window in the lid to be sure that the rotor has come to a standstill.
x Insert the hexagonal wrench key into the bore hole (Fig. 1, A) and carefully rotate by half a turn in clockwise
direction until the lid can be opened.
x Pull the hexagon socket head wrench out of the drilling again.
x If the left LED in the button flashes after the centrifuge is switched on again, press the
button so that the motor-driven lid lock goes into the basic position (opened) again.

AB2400EN_SA Rev. 02 / 12.2022 19/27
24 Maintenance and servicing
The device can be contaminated.
Pull the mains plug before cleaning.
Before any other cleaning or decontamination process other than that recommended by the manufacturer is
applied, the user has to check with the manufacturer that the planned process does not damage the device.
x Centrifuges, rotors and accessories must not be cleaned in rinsing machines.
x They may only be cleaned by hand and disinfected with liquids.
x The water temperature must be between 20 – 25°C.
x Only detergents/disinfectants may be used which:
 have a pH between 5 - 8
 do not contain caustic alkalis, peroxides, chlorine compounds, acids and alkaline solutions
x In order to prevent appearances of corrosion through cleaning agents or disinfectants, the application guide from
the manufacturer of the cleaning agent or disinfectant are absolutely to be heeded.
24.1 Centrifuge (housing, lid and centrifuging chamber)
24.1.1 Surface cleaning and care
x Clean the centrifuge housing and the centrifuging chamber regularly, using soap or a mild detergent and a damp
cloth if required. For one thing, this services purposes of hygiene, and it also prevents corrosion through
adhering impurities.
x Ingredients of suitable detergents:
soap, anionic tensides, non-ionic tensides.
x After using detergents, remove the detergent residue by wiping with a damp cloth.
x The surfaces must be dried immediately after cleaning.
x In the event of condensation water formation, dry the centrifugal chamber by wiping out with an absorbent cloth.
x Lightly rub the rubber seal of the centrifuge chamber with a rubber care product after each cleaning.
x The centrifuging chamber is to be checked for damage once a year.
If damage is found which is relevant to safety, the centrifuge may no longer be put into operation. In this
case, notify Customer Service.
24.1.2 Surface disinfection
x If infectious materials penetrates into the centrifugal chamber this is to be disinfected immediately.
x Ingredients of suitable disinfectants:
ethanol, n-propanol, ethyl hexanol, anionic tensides, corrosion inhibitors.
x After using disinfectants, remove the disinfectant residue by wiping with a damp cloth.
x The surfaces must be dried immediately after disinfecting.
24.1.3 Removal of radioactive contaminants
x The agent must be specifically labelled as being an agent for removing radioactive contaminants.
x Ingredients of suitable agents for removing radioactive contaminants:
anionic tensides, non-ionic tensides, polyhydrated ethanol.
x After removing the radioactive contaminants, remove the agent residue by wiping with a damp cloth.
x The surfaces must be dried directly after removing the radioactive contaminants.

20/27 Rev. 02 / 12.2022 AB2400EN_SA
24.2 Rotors and Attachments
24.2.1 Cleaning and care
x In order to avoid corrosion and changes in materials, the rotors and accessories have to be cleaned regularly
with soap or with a mild cleaning agent and a moist cloth. Cleaning is recommended at least once a week.
Contaminants must be removed immediately.
x Ingredients of suitable detergents:
soap, anionic tensides, non-ionic tensides.
x After using detergents, remove detergent residue by rinsing with water (only outside of the centrifuge) or wipe off
with a damp cloth.
x The rotors and accessories must be dried directly after cleaning.
x Angle rotors, container and hanger made of aluminium are to be lightly greased after drying using acid-free
grease, e.g. vaseline.
x In the case of biosafety systems (for further details of available biosafety systems see chapter
"Anhang/Appendix, Rotoren und Zubehör/Rotors and accessories"), the packing rings must be checked and
cleaned regularly (weekly).The sealing ring is to be replaced immediately upon indication of crack formation,
embrittlement or abrasive wear. In order to prevent the packing ring from twisting when opening and closing the
cover, the packing ring must be lightly rubbed with a rubber care product.
x In order to prevent corrosion as a result of moisture between the rotor and the motor shaft, the rotor should be
disassembled and cleaned at least once a month, and the motor shaft should be lightly greased.
x The rotors and accessories have to be checked monthly for wear and tear and damage due to corrosion.
Rotors and attachments may no longer be utilised upon indication of wear and tear or corrosion.
x Check the firm seating of the rotor on a weekly basis.
24.2.2 Disinfection
x If infectious material should get on the rotors or accessories, they must be appropriately disinfected.
x Ingredients of suitable disinfectants:
ethanol, n-propanol, ethyl hexanol, anionic tensides, corrosion inhibitors.
x After using disinfectants, remove disinfectant residue by rinsing with water (only outside of the centrifuge) or wipe
off with a damp cloth.
x The rotors and accessories must be dried directly after disinfection.
24.2.3 Removal of radioactive contaminants
x The agent must be specifically labelled as being an agent for the removal of radioactive contaminants.
x Ingredients of suitable agents for removing radioactive contaminants:
anionic tensides, non-ionic tensides, polyhydrated ethanol.
x After removing the radioactive contaminants, remove agent residue by rinsing with water (only outside of the
centrifuge) or wipe off with a damp cloth.
x The rotors and accessories must be dried directly after removing the radioactive contaminants.
24.2.4 Rotors and accessories with limited service lives
The use of certain rotors, hangers and accessory parts is limited by time.
These are marked with the maximum permitted number of operating cycles or with an expiration date and the
maximum permitted number of operating cycles or just with the expiration date; e.g.:
- "einsetzbar bis Ende: ,V. Quartal 2011 / usable until end of: ,V. Quarter 2011" or
"einsetzbar bis Ende Monat/Jahr: 10/2011 / usable until end of month/year: 10/2011"
- "Max. Lauf Zyklen / max. cycles: 40000".
For safety reasons, rotors, hangers and accessory parts may no longer be used if either the indicated
maximum number of operating cycles or the indicated expiration date has been reached.