Beckman Coulter Optima XE Series User manual

PN B07711AA
May 2011
User Guide
Optima XE Series
Preparative Ultracentrifuges
For non-In Vitro Diagnostic Use
Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821

Optima XE
Ultracentrifuge
User Guide
PN B07711AA (May 2011)
© 2011 Beckman Coulter, Inc.
All Rights Reserved
Find us on the World Wide Web at:
www.beckmancoulter.com
Beckman Coulter Ireland, Inc.
Mervue Business Park, Mervue Galway, Ireland 353 91 774068
Beckman Coulter do Brasil Com e Imp de Prod de Lab Ltda
Estr dos Romeiros, 220 - Galpao G3 - Km 38.5
06501-001 - Sao Paulo - SP - Brasil
CNPJ: 42.160.812/0001-44
製造販売元 : ベックマン･コールター株式会社
東京都江東区有明三丁目5番7号
TOC 有明ウエストタワー
贝克曼库尔特有限公司，
美国加利福尼亚州，Brea 市，S. Kraemer 大街 250 号，
邮编：92821 电话：[phone]

iii
Contents
CHAPTER 1: Safety and Notices,1-1
Alerts for Danger, Warning, Caution, Important, and Note,1-1
Safety Notices,1-2
Information,1-2
Installation,1-2
Replacement Parts,1-3
Service,1-3
Power Supply,1-3
Fuse Replacement,1-3
Mechanical Safety,1-4
Chemical and Biological Safety,1-4
Precautions with Liquids,1-5
Volatile Liquids,1-5
Certification,1-5
Scope of Manual,1-5
CFC-Free Centrifugation,1-6
Radio Interference,1-6
United States FCC Certification,1-6
Canadian Department of Communications Certification,1-6
Industry Canada,1-6
Software Copyright,1-7
Summary of Instrument Labels,1-7
Caution Symbol,1-7
RoHS Caution,1-7
Universal Serial Bus (USB),1-8
Ethernet,1-8
Recycling Label,1-8
Multiple Compliance,1-9
CAUTION Static Sensitive Area,1-9
DANGER High Voltage,1-9
Protective Ground,1-10
Rotor Rotation,1-10
Symbols,1-11

iv
Contents
CHAPTER 2: Description,2-1
For non-In Vitro Diagnostic Use,2-1
Touch Screen,2-1
Name Rating Plate,2-1
Rotor Chamber,2-2
Vacuum System,2-2
Temperature Sensing and Control,2-2
Drive,2-2
Safety Features,2-3
Door,2-3
Barrier Ring,2-3
Imbalance Detector,2-3
Overspeed System,2-3
Dynamic Rotor Inertia Check (DRIC),2-3
Specifications,2-4
Control Features,2-4
Physical Data,2-5
Audible Sounds,2-6
Available Rotors,2-7
CHAPTER 3: The Touch Screen Interface,3-1
Areas on the Screen,3-1
Header Bar,3-2
Home Page Button,3-2
Menu Button,3-2
Status Display,3-3
Help Button,3-4
Footer Bar,3-4
Start Button,3-4
Stop Button,3-4
Footer Bar on the Home Page,3-5
Footer Bar on Other Pages,3-5
Vacuum Display/Button,3-5
System Name,3-5
Accel and Decel Display/Buttons,3-6
Page Display Area,3-6
Help Messages,3-6
Item Help,3-6
Global Help,3-7
CHAPTER 4: Operations,4-1
Manual Operation,4-1
Step 1: Start on the Home Page,4-2

v
Contents
Step 2: Set the Speed and Rotor,4-3
Step 3: Set the Time,4-5
Step 4: Set the Temperature,4-6
Step 5: Start the Run,4-7
Zonal and Continuous Flow Operation,4-7
Preparing for the Run,4-8
Starting the Run,4-9
Loading the Sample,4-9
Running the Sample,4-10
Unloading the Sample,4-10
Stopping the Run,4-10
Finishing the Run,4-10
CHAPTER 5: Functional Pages,5-1
Home Page,5-2
w²t Display,5-2
Set Speed Display/Button,5-3
Set Time Display/Button,5-3
Set Temp Display/Button,5-3
Menu Page,5-4
Set Speed Page,5-5
Set Time Page,5-6
Set Speed Time Page (w²t Mode),5-7
Set Temperature Page,5-8
Set Acceleration/Deceleration Profiles Page,5-9
Example Acceleration and Deceleration Profiles,5-10
Select Rotor and Labware,5-11
References Page,5-12
System Options Page,5-13
Zonal Authorization Page,5-14
Continuous Flow Authorization Page,5-15
Service Login Page,5-16
About Page,5-17
Rotor Catalog Page,5-18
Labware Catalog Page,5-19
Export User Manual Page,5-20
Set System Name Page,5-21
Set Date and Time Page,5-22
Set Sound Page,5-23

vi
Contents
Select Language Page,5-24
Diagnostic History Page,5-25
Compatible Tubes for Rotor Page,5-26
Zonal Operation Page,5-27
Continuous Flow Operation Page,5-28
Running Home Page,5-29
CHAPTER 6: Maintenance and Troubleshooting,6-1
Field Service,6-1
Rotors and Labware,6-1
Cleaning,6-1
Instrument Surfaces,6-2
Rotor Chamber,6-2
Chamber Door O-ring,6-2
Decontamination,6-2
Sterilization and Disinfection,6-3
Diagnostics/User Messages,6-3
Retrieving Your Sample in Case of Power Failure,6-3
During a Run,6-3
Rotor Spinning at Restoration,6-3
Rotor Stopped at Restoration,6-4
Getting Access to the Sample,6-4
Restoring the Instrument to Operating Condition,6-8
Storage and Transportation,6-8
Supply List,6-9
Replacement Parts,6-9
Supplies,6-9
APPENDIX A: Preinstallation Requirements,A-1
Overview,A-1
Space Requirements,A-1
Safety,A-1
Ventilation,A-2
Temperature,A-2
Electrical Requirements,A-3
APPENDIX B: Special Warranty for the Optima™ XE,B-1
Special Warranty,B-1

vii
Contents
APPENDIX C: Diagnostics,C-1
Overview,C-1
Diagnostics/User Messages Chart,C-1

viii
Contents

PN B07711AA 1-1
CHAPTER 1
Safety and Notices
This chapter presents the important notices that apply to the instrument and describes the
conventions used in the document.
Alerts for Danger, Warning, Caution, Important, and Note
DANGER
DANGER indicates an imminently hazardous situation which, if not avoided, will
result in death or serious injury. This signal word is to be limited to the most
extreme situations.
WARNING
WARNING indicates a potentially hazardous situation which, if not avoided, could
result in death or serious injury. May be used to indicate the possibility of
erroneous data that could result in an incorrect diagnosis (does not apply to all
products).
CAUTION
CAUTION indicates a potentially hazardous situation, which, if not avoided, may
result in minor or moderate injury. It may also be used to alert against unsafe
practices. May be used to indicate the possibility of erroneous data that could
result in an incorrect diagnosis (does not apply to all products).
IMPORTANT IMPORTANT is used for comments that add value to the step or procedure being performed.
Following the advice in the Important adds benefit to the performance of a piece of equipment or to a
process.
NOTE NOTE is used to call attention to notable information that should be followed during installation, use,
or servicing of this equipment.

PN B07711AA
1-2
Safety and Notices
Safety Notices
Safety Notices
Before installing, using, or maintaining the instrument, be certain that you know all the following
precautions.
Information
CAUTION
Read all product manuals and consult with Beckman Coulter-trained personnel
before attempting to operate instrument. Do not attempt to perform any
procedure before carefully reading all instructions. Always follow product labeling
and manufacturer’s recommendations. If in doubt as to how to proceed in any
situation, contact your Beckman Coulter Representative.
Installation
CAUTION
This instrument is designed to be installed by a Beckman Coulter Field Service
representative. Installation by anyone other than authorized Beckman Coulter
personnel invalidates any warranty covering the instrument. Also, should the
instrument need to be moved, a Beckman Coulter Field Service representative
must reinstall and re-level the instrument in its new location.
WARNING
Do not place the ultracentrifuge near areas containing flammable or combustible
fluids, or any other source of vapors that could enter the ultracentrifuge air
system and be ignited by the motor.
WARNING
Ultracentrifuge operations generate high energy levels that require precautions
against sudden movements that could result from the rare event of a rotor failure.
Maintain a 30-cm. (1-ft.) clearance envelope around and above the
ultracentrifuge. Do not install any equipment or furniture in this envelope. While
the ultracentrifuge is running, keep the envelope clear of any persons or objects
and do not reach into the envelope except when required to change operating
controls.

PN B07711AA 1-3
Safety and Notices
Safety Notices 1
Replacement Parts
WARNING
Do not replace any centrifuge components with parts not specified for use on this
instrument.
Service
WARNING
Any servicing of this equipment that requires removal of any covers can expose
parts which involve the risk of electric shock or personal injury. Make sure that the
power switch is turned off, and the instrument is disconnected from the main
power source, by removing its power plug from the receptacle. Refer such
servicing to qualified personnel.
NOTE It is your responsibility to decontaminate the instrument and accessories before requesting service by
Beckman Coulter Field Service.
Power Supply
DANGER
To reduce the risk of electrical shock, this instrument uses a three-wire electrical
cord and plug to connect this equipment to earth-ground. Make sure that the
matching wall outlet receptacle is properly wired and earth-grounded.
Fuse Replacement
WARNING
Fuses protect certain electrical circuits within this instrument against overcurrent
conditions. The fuse is not customer replaceable. For continued protections,
please contact Beckman Coulter Field Service.

PN B07711AA
1-4
Safety and Notices
Safety Notices
Mechanical Safety
DANGER
For safe operation of the equipment, observe the following:
• Use only the Beckman Coulter rotors and accessories designed for use in this
instrument.
• Do not exceed the maximum rated speed of the rotor in use.
• NEVER attempt to slow or stop a rotor by hand.
• Do not move the centrifuge while the drive motor is spinning.
• In the event of a power failure, do not attempt to retrieve the sample from the
instrument for at least one hour. Then follow the instructions for recovery of
the sample in the Maintenance and Troubleshooting chapter.
Chemical and Biological Safety
DANGER
Normal operation may involve the use of solutions and test samples that are
pathogenic, toxic, or radioactive. Such materials require that you take all
necessary safety precautions.
• Handle body fluids with care because they can transmit disease. No known
test offers complete assurance that they are free of micro-organisms.
• Handle all infectious samples according to good laboratory procedures and
methods to prevent spread of disease.
• Because spills may generate aerosols, observe proper safety precautions for
aerosol containment.
• Some of the most virulent infectious agents—Hepatitis (B and C) and HIV (I-V)
viruses, atypical mycobacteria, and certain systemic fungi—require
additional emphasis on aerosol protection.
• Do not run toxic, pathogenic, or radioactive materials in a rotor without taking
appropriate safety precautions.
• Risk Group II materials (as identified in the World Health Organization
Laboratory Biosafety Manual) require biosafe containment. Materials of a
higher group require more than one level of protection.
• Dispose of all waste solutions according to appropriate environmental health
and safety guidelines.

PN B07711AA 1-5
Safety and Notices
Certification 1
Precautions with Liquids
WARNING
Do not place containers holding liquid on or near the chamber door. Liquid, if
spilled, may get into the instrument and damage electrical or mechanical
components.
Volatile Liquids
DANGER
This instrument is not designed for use with materials capable of developing
flammable or explosive vapors. Do not centrifuge such materials (for example,
chloroform or ethyl alcohol) in this instrument nor handle or store them near the
centrifuge.
Certification
To ensure full system quality, Beckman Coulter Optima XEs have been manufactured in a registered
ISO 9001 or 13485 facility. They have been designed and tested to be compliant (when used with
Beckman Coulter rotors) with the laboratory equipment requirements of applicable regulatory
agencies. Declarations of conformity and certificates of compliance are available at
www.beckmancoulter.com.
Scope of Manual
This manual is designed to familiarize you with the Optima XE, its functions, specifications,
operation, and routine operator care and maintenance. Read this entire manual, especially the
safety notices and all safety-related information, before operating the instrument or performing
maintenance.
NOTE If the instrument is used in a manner other than specified in this manual, the safety and performance
of this equipment could be impaired. Further, the use of any equipment other than that recommended
by Beckman Coulter has not been evaluated for safety. Use of any equipment not specifically
recommended in this manual and/or the appropriate rotor manual is the sole responsibility of the user.

PN B07711AA
1-6
Safety and Notices
CFC-Free Centrifugation
CFC-Free Centrifugation
To ensure minimal environmental impact, no CFCs are used in the manufacture or operation of the
Optima XE ultracentrifuge.
Radio Interference
This instrument is certified with regard to radio frequency interference for both the United States
and Canada.
United States FCC Certification
This equipment has been tested and found to comply with the limits for a Class A digital device,
pursuant to Part 15 of FCC Rules. These limits are designed to provide reasonable protection against
harmful interference when the equipment is operated in a commercial environment. This
equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in
accordance with this instruction manual, may cause interference to radio communications.
Operation of this equipment in a residential area is likely to cause interference, in which case the
user will be required to correct the interference at his or her own expense.
Canadian Department of Communications Certification
This equipment does not exceed the Class A limits for radio noise emissions from digital apparatus
as set out in the radio interference regulations of the Canadian Department of Communications.
Le présent appareil numérique n’émet pas de bruits radioélectriques dépassant les limites
applicables aux appareils numériques de Classe A prescrites dans le reglement sur le brouillage
radioelectrique édicté par le Ministère des Communications du Canada.
Industry Canada
This Class A digital apparatus complies with Canadian ICES-003.
Cet appareil numérique de la classe A est conforme à la norme NMB-003 du Canada.

PN B07711AA 1-7
Safety and Notices
Software Copyright 1
Software Copyright
The software and other information incorporated into the Optima XE is protected by international
copyright laws. Unauthorized copying, use, distribution, transfer, or sale is a violation of those laws
that may result in civil or criminal penalties. This computer program is also subject to additional
restrictions contained in the following Microsoft OEM Customer License Agreement for Embedded
Systems:
“If you use the Device to access or utilize the services or functionality of Microsoft Windows XP
Server (all editions), or use the Device to permit workstation or computing devices to access or
utilize the services or functionality of Microsoft Windows XP Server, you may be required to obtain
a Client Access License for the Device and/or each such workstation or computing device. Refer to
the End-User License Agreement for Microsoft Windows XP Server for additional information.”
The End-User License Agreement is available on the Microsoft Embedded Systems website.
Summary of Instrument Labels
This section provides information for some labels and symbols appearing on the Optima XE
instrument housing. These labels and symbols may be associated with user-serviceable procedures.
Individual hazards associated with a specific procedure in this manual may use these labels and
symbols, and are included in Warnings or Cautions within the procedures for that task.
Caution Symbol
This symbol indicates a caution message and appears adjacent to an explanation or other symbols
that define the caution.
RoHS Caution
This label and materials declaration table (the Table of Hazardous Substance's Name and
Concentration) are to meet People's Republic of China Electronic Industry Standard SJ/T11364-2006
“Marking for Control of Pollution Caused by Electronic Information Products” requirements.
!

PN B07711AA
1-8
Safety and Notices
Summary of Instrument Labels
This logo indicates that this electronic information product contains certain toxic or hazardous
elements, and can be used safely during its environmental protection use period. The number in the
middle of the logo indicates the environmental protection use period for the product. The outer
circle indicates that the product can be recycled. The logo also signifies that the product should be
recycled immediately after its environmental protection use period has expired. The date on the
label indicates the date of manufacture.
Universal Serial Bus (USB)
This symbol indicates the location of a universal serial bus (USB) connector.
Ethernet
This symbol indicates the location of an ethernet connector.
Recycling Label
This symbol is required in accordance with the Waste Electrical and Electronic Equipment (WEEE)
Directive of the European Union. The presence of this marking on the product indicates:
• the device was put on the European Market after August 13, 2005 and
• the device is not to be disposed of via the municipal waste collection system of any member
state of the European Union.
It is very important that customers understand and follow all laws regarding the proper
decontamination and safe disposal of electrical equipment. For Beckman Coulter products bearing
this label, please contact your dealer or local Beckman Coulter office for details on the take-back
program that will facilitate the proper collection, treatment, recovery, recycling and safe disposal
of the device.
A28219-AA

PN B07711AA 1-9
Safety and Notices
Summary of Instrument Labels 1
Multiple Compliance
This symbol indicates compliance with:
• certain US Federal Communications Commission Part 15 Rules as described on the label
• N395 – The C-Tick mark is intended for use on products that comply with Australian
Communication Authority (ACA) EMC Requirements.
• 169502 – This label indicates recognition by a Nationally Recognized Testing Laboratory (NRTL)
that the instrument has met the relevant product safety standards.
• CE – This label indicates conformance to various Directives set forth under European Union law.
• Recycling – Refer to the Recycling Label section in this document.
CAUTION Static Sensitive Area
Indicates an area of the instrument which is sensitive to static electrostatic discharge (ESD). To
prevent damage due to electrostatic discharge, always wear a properly earth-grounded wrist strap
while operating this instrument. For details on proper grounding, see IEEE standard P1100.
DANGER High Voltage
Operation, replacement or servicing of any components where contact with bare, live hazardous
parts could occur, possibly resulting in electric shock, should only be performed by your
Beckman Coulter representative.

PN B07711AA
1-10
Safety and Notices
Summary of Instrument Labels
Protective Ground
This symbol is used to indicate a protective ground. This instrument must be properly grounded.
Do not under any circumstances operate the instrument unless it is properly grounded.
Rotor Rotation
This indicates the direction of instrument rotor rotation.

PN B07711AA 1-11
Safety and Notices
Symbols 1
Symbols

PN B07711AA
1-12
Safety and Notices
Symbols