Hettich ROTINA 420 User manual

Rev. 03 / 11.2022 Andreas Hettich GmbH & Co. KG AB4701EN_SA
ROTINA 420
ROTINA 420 R
EN
Operating Instructions.................................................... 4

2/65 Rev. 03 / 11.2022 AB4701EN_SA
A
Fig. 1
PROG RCF RPM t/min
PROG RCF RPM TIME
START
STOP
OPEN
12:304500
199
Fig. 2 ROTINA 420
PROG T/°C RCF RPM t/min
PROG T/°C RCF RPM TIME
START
STOP
OPEN
12:30450020
199
Fig. 3 ROTINA 420 R

AB4701EN_SA Rev. 03 / 11.2022 3/65
Andreas Hettich GmbH & Co. KG
Föhrenstraße 12, D-78532 Tuttlingen / Germany
Phone [phone] / 705-0
Fax [phone]
[email protected], servi[email protected]
www.hettichlab.com
Single Registration Number: DE-MF-000010680
© 2006 by Andreas Hettich GmbH & Co. KG
All rights reserved. No part of this publication may be reproduced without the prior written permission of the copyright
owner.
Änderungen vorbehalten! , Modifications reserved! , Sous réserve de modifications ! , Con riserva di modifiche!
AB4701EN_SA / Rev. 03

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4/65 Rev. 03 / 11.2022 AB4701EN_SA
Contents
1Use according to specification ................................................................................................................................7
2Remaining risks ......................................................................................................................................................7
3Technical specifications..........................................................................................................................................8
4Notes on safety.......................................................................................................................................................9
5Symbol meanings .................................................................................................................................................11
6Delivery checklist..................................................................................................................................................12
7Transport and storage ..........................................................................................................................................12
7.1 Transport .......................................................................................................................................................12
7.2 Storage..........................................................................................................................................................12
8Unpacking the centrifuge ......................................................................................................................................12
9Initial operation .....................................................................................................................................................13
10 Interface (only for centrifuge with interface) ......................................................................................................13
11 Opening and closing the lid ...............................................................................................................................13
11.1 Opening the lid...........................................................................................................................................13
11.2 Closing the lid ............................................................................................................................................13
12 Installation and removal of the rotor ..................................................................................................................14
13 Inserting and removing hangers into/from the rotor...........................................................................................15
14 Inserting and removing the adapters into/from the hangers ..............................................................................15
15 Loading the rotor ...............................................................................................................................................16
16 Closing biosafety systems.................................................................................................................................17
17 Control and display elements ............................................................................................................................18
17.1 Control knob...............................................................................................................................................18
17.2 Keys and setting options............................................................................................................................18
18 Enter centrifugation parameters ........................................................................................................................19
18.1 Running time..............................................................................................................................................20
18.2 Starting runtime timing ...............................................................................................................................20
18.3 Speed (RPM) .............................................................................................................................................20
18.4 Relative centrifugal force (RCF) and centrifuging radius (RAD).................................................................20
18.5 Start-up and run-down parameters ............................................................................................................21
18.5.1 Start-up stage and start-up time .........................................................................................................21
18.5.2 Braking stage and run-down time .......................................................................................................21
18.5.3 Brake switch-off speed .......................................................................................................................21
18.6 Temperature (only for centrifuge with refrigeration) ...................................................................................21
19 Programming.....................................................................................................................................................22
19.1 Inputting or changing programs .................................................................................................................22
19.2 Calling up programs...................................................................................................................................22
19.3 Write protection for programs.....................................................................................................................22
19.4 Program linkage.........................................................................................................................................23
19.4.1 Activating or deactivating program linkage .........................................................................................23
19.4.2 Linking programs or changing a program linkage ...............................................................................23
19.4.3 Calling up program linkage .................................................................................................................24
19.5 Automatic temporary storage.....................................................................................................................24
20 Centrifugation....................................................................................................................................................24

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20.1 Centrifugation with preselected time ..........................................................................................................24
20.2 Continuous operation.................................................................................................................................25
20.3 Short-time centrifugation ............................................................................................................................25
21 Change the settings during the centrifugation run .............................................................................................25
22 Integral RCF......................................................................................................................................................26
22.1 Query integral RCF ....................................................................................................................................26
22.2 Activate or deactivate the integral RCF display..........................................................................................26
23 Emergency Stop................................................................................................................................................26
24 Cycle counter ....................................................................................................................................................27
24.1 After starting the first centrifugation run, enter the maximum permitted number of rotor cycles or deactivate
the cycle counter ......................................................................................................................................................27
24.2 Reset cycle counter to "0" and enter the maximum permitted number of rotor cycles ...............................28
24.3 Activating or deactivating the cycle counter ...............................................................................................28
25 Activating/deactivating the "Dual time mode" function" .....................................................................................29
26 Activating or deactivating start-up and run-down times .....................................................................................29
27 Acoustic signal ..................................................................................................................................................30
28 Displayed centrifugation data after switch-on....................................................................................................30
29 Set temperature unit (only for centrifuge with refrigeration)...............................................................................31
30 Backlighting of the display.................................................................................................................................31
31 Setting program locks........................................................................................................................................32
32 PIN (Personal Identification Number) ................................................................................................................33
32.1 Setting or changing the PIN .......................................................................................................................33
32.2 Procedure in the event that PIN is lost .......................................................................................................33
33 Centrifuge address ............................................................................................................................................34
34 Query the operating hours, centrifugation runs and cycle counter ....................................................................34
35 Query the system information............................................................................................................................34
36 Immediate display of centrifugation data after switch-on...................................................................................34
37 Cooling (only in centrifuges with cooling) ..........................................................................................................35
37.1 Standby-cooling .........................................................................................................................................35
37.2 Precooling of the rotor................................................................................................................................35
37.3 Time-controlled cooling ..............................................................................................................................35
37.4 Prevent cooling being switched on during run-out .....................................................................................36
37.5 Temperature monitoring.............................................................................................................................36
38 Heating (only on centrifuges with heating/cooling option) .................................................................................37
39 Relative centrifugal force (RCF) ........................................................................................................................37
40 Centrifugation of materials or mixtures of materials with a density higher than 1.2 kg/dm3...............................38
41 Rotor recognition ...............................................................................................................................................38
42 Emergency release ...........................................................................................................................................38
43 Maintenance and servicing................................................................................................................................39
43.1 Centrifuge (housing, lid and centrifuging chamber)....................................................................................39
43.1.1 Surface cleaning and care ..................................................................................................................39
43.1.2 Surface disinfection.............................................................................................................................39
43.1.3 Removal of radioactive contaminants .................................................................................................39
43.2 Rotors and Attachments.............................................................................................................................40
43.2.1 Cleaning and care...............................................................................................................................40

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43.2.2 Disinfection .........................................................................................................................................40
43.2.3 Removal of radioactive contaminants .................................................................................................40
43.2.4 Trunnions............................................................................................................................................41
43.2.5 Rotors and accessories with limited service lives ...............................................................................41
43.3 Autoclaving ................................................................................................................................................41
43.4 Centrifuge containers.................................................................................................................................41
44 Faults ................................................................................................................................................................42
45 Switch on automatic circuit breaker...................................................................................................................44
46 Returning Devices.............................................................................................................................................44
47 Disposal ............................................................................................................................................................44
48 Anhang / Appendix............................................................................................................................................45
48.1 Rotoren und Zubehör / Rotors and accessories.........................................................................................45

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1 Use according to specification
The centrifuge is an in vitro diagnostic medical device according to the in vitro diagnostic medical devices regulation
(EU) 2017/746.
The centrifuge was designed only for the separation of materials or mixtures with a density of no more than 1.2
kg/dm³. In particular, these include samples for preparation of in-vitro diagnostic purposes in human medicine.
The centrifuge is only meant for this purpose.
Another use or one which goes beyond this, is considered to be non-intended. The company Andreas Hettich GmbH
& Co. KG is not liable for damage resulting from this.
Observing all information in the operating instructions and complying with the measures described therein is also a
part of the intended use.
2 Remaining risks
The device is built according to the state-of-the-art and the recognized safety regulations. If used and handled
improperly, there could be life-threatening danger to the user or third parties, or the device could be impaired or there
could be other property damage. The device is only to be used for its intended purpose and only when it is in safe
working condition.
Malfunctions which could affect safety must be corrected immediately.

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3 Technical specifications
Manufacturer Andreas Hettich GmbH & Co. KG
D-78532 Tuttlingen
Model ROTINA 420 ROTINA 420 R
Basic UDI-DI 4050674010015QB
Type 4701 4701-01
4706,
4706-20,
4706-50
4706-07 4706-01
Mains voltage (10%) 200 – 240 V
1
100 – 127 V
1
200 – 240 V
1
100–127V
1
100 V
1
Mains frequency 50 – 60 Hz 50 – 60 Hz 50 Hz 60 Hz 60 Hz 50 Hz
Connected load 870 VA 900 VA 1600 VA 1300 VA 1850 VA
Current consumption 4.3 A 9.0 A 7.5 A 16.0 A
Cooling medium ---- R452A
Max. capacity 4 x 600 ml
Allowed density 1.2 kg/dm3
Speed (RPM) 15000
Force (RCF) 24400
Kinetic energy 24000 Nm
Obligatory inspection
(DGUV Regel 100 – 500)
yes
Ambient conditions (EN / IEC 61010-1)
 Set-up site Indoors only
 Altitude Up to 2000 m above sea level
 Ambient temperature 2°C to 35°C 5°C to 35°C
 Humidity Maximum relative humidity 80% for temperatures up to 31°C, linearly
decreasing to 50% relative humidity at 40°C.
 Excess-voltage category
(IEC 60364-4-443) 
 Pollution degree 2
Device protection class 
Not suitable for use in explosion-endangered areas.
EMC
 Emitted interference,
Interference immunity
EN / IEC
61326-1,
Class B
FCC Class
B
EN / IEC 61326-1,
Class B
FCC Class B
Noise level (dependent on rotor) 63 dB(A) 58 dB(A) 64 dB(A) 58 dB(A)
Dimensions
 Width 506 mm 713 mm
 Depth 650 mm 654 mm
 Height 423 mm 423 mm
Weight approx. 75 kg approx. 84 kg approx. 107.5 kg,
109 kg (4706-50)
approx. 117 kg

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4 Notes on safety
No claim of warranty will be considered by the manufacturer unless ALL instructions in this manual
have been followed.
Reports of serious incidents involving the device
Report any serious incidents involving the device to the manufacturer and, if necessary, to the competent
authority.
 The centrifuge should be installed on a good, stable base.
 Before using the centrifuge absolutely check the rotor for firm placement.
 When the centrifuge is running, according to EN / IEC 61010-2-020, no persons, dangerous
substances or objects may be within the safety margin of 300 mm around the centrifuge.
 Rotors, suspensions and accessories that possess traces of corrosion or mechanical damage or
if their term of use has expired may not be used any longer.
 The centrifuge may no longer be put into operation when the centrifuging chamber has safety-
related damages.
 With swing-out rotors the trunnions must be regularly lubricated (Hettich Lubricating Grease No.
4051) in order to ensure consistent swinging out of the hangers.
 For centrifuges without temperature control, when the room temperature is increased and/or if the
device is frequently used, the centrifuging chamber could be heated up. Therefore, it can't be
ruled out that the sample material might be changed due to the temperature.
 Before the initial operation of your centrifuge you should read and pay attention to the operating
instructions. Only personnel that has read and understood the operating instructions are allowed to
operate the device.
 Along with the operating instructions and the legal regulations on accident prevention, you should also follow the
recognised professional regulations for working in a safe and professional manner. These operating instructions
should be read in conjunction with any other instructions concerning accident prevention and environmental
protection based on the national regulations of the country where the device is to be used.
 This centrifuge is a state-of-the-art piece of equipment which is extremely safe to operate. However, it can lead to
danger for users or others if used by untrained staff, in an inappropriate way or for a purpose other than that it
was designed for.
 The centrifuge must not be moved or knocked during operation.
 In case of fault or emergency release, never touch the rotor before it has stopped turning.
 To avoid damage due to condensate, when changing from a cold to a warm room the centrifuge must either heat
up for at least 3 hours in the warm room before being connected to the mains, or run hot for 30 minutes in the
cold room.
 Only the rotors and accessories approved by the manufacturer for this device may be used (see chapter
"Anhang/Appendix, Rotoren und Zubehör/Rotors and accessories"). Before centrifuge vessels are used which are
not listed in the chapter "Appendix, Rotors and accessories", the user must make sure they can be used by
asking the manufacturer.
 The centrifuge rotor may only be loaded in accordance with the chapter "Loading the rotor".
 When centrifuging with maxim revolutions per minute the density of the materials or the material mixtures may not
exceed 1.2 kg/dm3.
 The centrifuge may only be operated when the balance is within the bounds of acceptability.
 The centrifuge may not be operated in explosion-endangered areas.
 The centrifuge must not be used with:
 inflammable or explosive materials
 materials that react with one another producing a lot of energy.

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 When centrifuging hazardous substances or mixtures, which are toxic, radioactive or contaminated with
pathogenic microorganisms, suitable measures must be taken by the user.
Fundamentally, centrifuge containers with special screw closures must be used for hazardous substances. For
materials of risk groups 3 and 4, in addition to sealable centrifuge containers, a bio-safety system must be used
(see the "Laboratory Bio-safety Manual" from the World Health Organization).
In a bio-safety system, a bio-seal (sealing ring) prevents droplets and aerosols from escaping.
If the hanger of a bio-safety system is used without the lid, the sealing ring must be removed from the hanger to
prevent damage to the sealing ring during the centrifugation run.
Damaged bio-safety systems are no longer microbiologically sealed.
If a bio-safety system is not used, a centrifuge is not microbiologically sealed for the purposes of the standard EN
/ IEC 61010-2-020.
When closing a bio-safety system, follow the instructions in the chapter "Closing biosafety systems".
For the available bio-safety systems, see the chapter "Appendix, Rotors and accessories". If in doubt, you can
get the information you need from the manufacturer.
 The centrifuge must not be operated with highly corrosive substances which could impair the mechanical integrity
of rotors, hangers and accessories.
 Repairs must only be carried out by personnel authorised to do so by the manufacturer.
 Only original spare parts and original accessories licensed by the Andreas Hettich GmbH & Co. KG company are
allowed to be utilised.
 The following safety regulations apply:
EN / IEC 61010-1 and EN / IEC 61010-2-020 as well as their national deviations.
 The safe operation and reliability of the centrifuge can only be guaranteed if:
 the centrifuge is operated in accordance with the operating instructions,
 the electrical installation on the site where the centrifuge is installed conforms to the demands of EN / IEC
stipulations.
 Meeting the country-specific requirements concerning occupational safety with regard to the use of laboratory
centrifuges at the workplaces provided for this purpose by the user is the responsibility of the user.

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5 Symbol meanings
Symbol on the device:
Attention, general hazard area.
Symbol on the device:
Observe operating instructions.
This symbol indicates that the user must observe the operating instructions provided.
Symbol in this document:
Attention, general hazard area.
This symbol refers to safety relevant warnings and indicates possibly dangerous situations.
The non-adherence to these warnings can lead to material damage and injury to personal.
Symbol on the device and in this document:
Beware of hot surface.
Nonobservance of this warning can lead to material damage and personal injury.
Symbol on the device and in this document:
Beware of biohazard.
Symbol on the device and in this document:
Plastic suspension can only be used in temperatures up to a maximum of 40°C / 104°F.
Nonobservance of this warning can lead to material damage and personal injury.
Symbol on the device:
Equipotential: Connector (PE connector) for potential equalization (only for centrifuge with PE
connector).
IOIOI
RS232
Symbol on the device:
RS232 interface (only for device with RS232 interface).
F2
Symbol on the device:
Automatic circuit breaker (only in centrifuges with a circuit breaker).
Symbol in this document:
This symbol refers to important circumstances.
Symbol on the device and in this document:
Symbol for the separate collection of electric and electronic devices according to the guideline
2002/96/EG (WEEE). The device belongs to Group 8 (medical devices).
Applies in the countries of the European Union, as well as in Norway and Switzerland.
Symbol on the shipping carton label:
This way up.
Symbol on the shipping carton label:
The shipping packaging must be transported and
handled within the indicated humidity range (10% - 80%).
Symbol on the shipping carton label:
The shipping packaging must be stored, transported and
handled within the temperature range shown (-20°C - +60°C).
Symbol on the shipping carton label:
The shipping packaging must be kept away from rain and
kept in a dry environment.

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Symbol on the shipping carton label:
Fragile, handle with care.
Symbol on the shipping carton label:
Stack limit. Maximum number of identical packages which may be stacked on the bottom package,
whereby “n“ stands for the number of permissible packages. The bottom package is not included in “n”.
6 Delivery checklist
1 Connecting cable
1 Hex. pin driver 2,5 mm
1 Hex. pin driver 5 mm
1 Lubricating grease for trunnions
1 Operating instructions
1 Notes on moving the equipment safely
The rotor(s) and associated accessories are included in the delivery in the quantity ordered.
7 Transport and storage
7.1 Transport
Before transporting the device, the transport securing device must be installed.
When the device and accessories are transported, the following ambient conditions must be complied with:
 Ambient temperature: -20°C to +60°C
 Relative humidity: 10% to 80%, non-condensing
7.2 Storage
The device and the accessories may only be stored in closed and dry rooms.
When the device and accessories are stored, the following ambient conditions must be complied with:
 Ambient temperature: -20°C to +60°C
 Relative humidity: 10% to 80%, non-condensing
8 Unpacking the centrifuge
 Lift the carton upward and remove the padding.

Do not lift by the front panel.
Observe the weight of the centrifuge, refer to chapter "Technical specifications".
Lift the centrifuge on both sides with an appropriate number of helpers and place it on the laboratory table.

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9 Initial operation
 Position the centrifuge in a stable and level manner in a suitable place. During set-up, the required safety
margin of 300 mm around the centrifuge is to be kept according to EN / IEC 61010-2-020.
When the centrifuge is running, according to EN / IEC 61010-2-020, no persons, dangerous
substances or objects may be within the safety margin of 300 mm around the centrifuge.
 Ventilation openings may not be blocked.
A distance of 300 mm must be maintained from the ventilation slots and openings of the centrifuge.
 In the case of the centrifuge, type 4706-20, connect the nitrogen supply according to the enclosed instruction
sheet AH4706-20XX.
It is mandatory that the centrifuge be connected according to the enclosed instruction sheet.
Make sure you observe the enclosed instruction sheet.
 Centrifuge with PE connector:
If required, connect the PE connector at the back of the device with an additional medical potential equalization
system.
 Centrifuge with RS232 interface:
Connect the RS232 centrifuge interface with an RS232 connecting cable (not included in the scope of delivery) to
the PC.
 Check whether the mains voltage tallies with the statement on the type plate.
 Connect the centrifuge with the power cord to a standard mains socket. For connection ratings refer to Chapter
"Technical specifications".
 Switch on the mains switch (switch position ""). The LEDs in the keys are blinking.
The following displays appear one after the other:
1. The centrifuge model
2. The program version
3. The rotorcode (rotor), the maximum rotor speed (Nmax) and the preset centrifuging radius (R) of the last rotor
that was recognized by rotor recognition.
4. OPEN OEFFNEN
 Open the lid.
The centrifuge data of the last used program or program 1 will be displayed.
 Remove the transport safety device (see instruction sheet on “Moving the equipment safely”).
10 Interface (only for centrifuge with interface)
As an option, the device can be equipped with an RS232 interface.
The RS232 interface is labeled with the
IOIOI
RS232
symbol.
The centrifuge can be controlled and data queried via this interface.
The LED in the
PROG
key lights up during data communication.
11 Opening and closing the lid
11.1 Opening the lid
The lid can only be opened when the centrifuge is switched on and the rotor is at rest. If it cannot be
opened under these circumstances, see the section on “Emergency release”.
 Press the button
OPEN / STOP
. The lid unlocks via the motor and the left LED in the pushbutton
OPEN / STOP
extinguishes.
11.2 Closing the lid
Do not put your fingers between lid and housing.
Do not bang the lid shut.
If the left LED in the
OPEN / STOP
button flashes, press the
OPEN / STOP
button so that the motor-driven lid
lock goes into the basic position (opened).
 Place the lid and lightly press down the front edge of the lid. The locking action is effected by motor. The left LED
in the button
OPEN / STOP
lights up.

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12 Installation and removal of the rotor
Rotor installation:
Dirt particles between the motor shaft and rotor prevent the rotor from
having a perfect seat and cause it to run unsteadily.
 Clean the motor shaft (C) and the bore of the rotor (A) and then apply a thin coat
of grease to the motor shaft.
 Place the rotor vertically onto the motor shaft. The driver of the motor shaft (D)
must be in the groove of the rotor (B). The groove alignment is marked on the
rotor.
 Tighten the clamping nut of the rotor by turning it in the clockwise direction
using the key included in delivery.
 Check the rotor to make sure it is seated firmly.
To ensure a tight fit of the rotor, the nut of the rotor must be hand-tightened.
A
B
C
D
Removing the rotor:
 Loosen the clamping nut by turning it in the counter clockwise direction up to the
lifting pressure point. After overcoming the lifting pressure point, the rotor is
released from the cone of the motor shaft.
 Turn the clamping nut until the rotor can be lifted up from the motor shaft.

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13 Inserting and removing hangers into/from the rotor
For swing-out rotors, all rotor places must be occupied with the
same hangers.
Certain hangers are marked with the number of the rotor place.
These hangers may only be inserted in the corresponding rotor
place.
Hangers which are labelled with a set number, e.g. S001/4,
may only be used in a set.
B
A
C
Inserting hangers in the rotor:
 Check the rotor to make sure it is seated firmly.
 Grease the lifting lug (C) (Hettich lubricating grease, no. 4051).
 Insert hangers (A) into the rotor. While doing so, make sure that the
lifting lugs (C) are in the grooves (B) of the hangers.
 Push the hangers downward as far as they can go.
Removing hangers from the rotor:
 Pull the hangers (A) straight upward and out of the rotor.
14 Inserting and removing the adapters into/from the hangers
Inserting the adapters into the hangers:
 Insert the adapters (A) horizontally into the hangers (B).
Remove the adapters from the hangers:
 Remove the adapters (A) upward and out of the hangers (B).
B
A
B
A

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15 Loading the rotor
Standard centrifuge containers of glass will not stand RCF values exceeding 4000 (DIN 58970, pg. 2).
 Check the rotor for firm seating.
 With swing-out rotors all rotor positions must be lined with identical hangers. Certain hangers are marked with
the number of the rotor position. These hangers may only be used in the respective rotor position. Hangers that
are marked with a set number (e.g. S001/4) may only be used in the set.
 The rotors and hangers may only be loaded symmetrically. The centrifuge containers have to be distributed
evenly on all rotor positions. For authorised combinations see Chapter "Anhang/Appendix, Rotoren und
Zubehör/Rotors and accessories".
In the case of angle rotors all possible rotor positions must be loaded, see chapter "Anhang/Appendix, Rotoren
und Zubehör/Rotors and accessories".
Rotor is loaded symmetrically. Not permitted!
Rotor is loaded asymmetrically.
Rotor is evenly loaded Not permitted!
Rotor is not evenly loaded
 On certain hangers, the weight of the maximum load or the weight of the maximum load and the maximum
weight of the completely loaded hanger is specified. This weight may not be exceeded. In case of exception, see
chapter " Centrifugation of materials or mixtures of materials with a density higher than 1.2 kg/dm3". The weight
specification of the maximum load includes the total weight of the adapter, centrifuge container and contents.
 In containers with rubber inserts, the same number of rubber inserts must always be among the centrifuge
containers.
 The centrifuge containers may only be filled outside of the centrifuge.

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 The maximum filling quantity for the centrifuge containers specified by the manufacturer must not be exceeded.
In the case of angle rotors, the centrifuging
vessels may only be filled so far that no fluid
can be expelled from them while the centrifuge
is running.
Fluid
Centrifugal force
 When loading the angle rotors, no liquid may get into the angle rotors or centrifuging chamber.
 When loading the hangers and swing-out rotors and when swivelling out the hangers while the centrifuge is
running, no liquid may enter the hangers or the centrifuging chamber.
 In order to maintain the weight differences within the centrifuge container as marginal as possible, a consistent
fill level in the containers is to be heeded.
16 Closing biosafety systems
In order to guarantee that it is sealed, the lid to the biosafety system has to be firmly closed.
In order to prevent the packing ring from twisting when opening and closing the cover, the packing ring must
be lightly rubbed with a rubber care product.
If the hanger of a biosafety system is used without the lid, the packing ring must be removed from the hanger
in order to prevent the packing ring from being damaged during the centrifugation run. Damaged packing rings
must not be used to seal the biosafety system.
For further details of available biosafety systems see chapter "Anhang/Appendix, Rotoren und Zubehör/Rotors
and accessories". If in doubt, you should obtain relevant information from the manufacturer.
Lid with screw top and hole in turning handle
 Place the lid centred on the rotor.
 Insert the supplied key into the hole in the handle and close the lid firmly by turning the key in
a clockwise direction.
Lid with screw closure
 Place the lid on the hanger.
 Tightly seal the lid by hand by turning it clockwise.

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18/65 Rev. 03 / 11.2022 AB4701EN_SA
17 Control and display elements
See figure on page 2.
Fig. 2: Display and control panel
17.1 Control knob
For setting the individual parameters.
Turning anticlockwise reduces the value. Turning clockwise increases the value.
17.2 Keys and setting options
TIME
 Running time, parameters t/hms.
h: hours. Adjustable from 1 h to 99 h, in 1 hour increments.
m: minutes. Adjustable from 1 min to 59 min, in 1 minute increments.
s: seconds. Adjustable from 1 s to 59 s, in 1 second increments.
 Continual running ""
 Set runtime timing This setting can only be made if the function "Dual time mode" is activated. See chapter
"Activate or deactivate function "Dual time mode". This function is activated ex works.
You can decide whether the runtime is timed immediately after the start of the centrifugation run or as
soon as the set speed is reached.
Timing begins at Start = The runtime begins to count immediately after the centrifugation run starts.
Timing begins at Speed = The runtime only begins to count after the set speed is reached.
This is indicated on the display by the symbol , to the left next to the time.
RPM
 Revolution, parameters rpm.
Adjustable from 50 rpm to a maximum rotor speed of the rotor (Nmax), in increments of 10. For maximum
rotor speed, see chapter "Anhang/Appendix, Rotoren und Zubehör/Rotors and accessories".
RCF
 Relative centrifugal force, parameter RCF.
The RCF is shown in brackets . The LED in the button lights up.
The figure that is adjustable is the figure that produces a revolution speed between 50 rpm and the
maximum rotor speed (Nmax). Adjustable in increments of 1.
 Centrifuging radius, parameters RAD.
Adjustable from 10 mm to 330 mm in 1 millimeter increments. For centrifuging radius, see chapter
"Anhang/Appendix, Rotoren und Zubehör/Rotors and accessories".
 Requesting information from integral RCF.
You can only query integral RCF if the integral RCF display is activated. See chapter "Activate or
deactivate integral RCF display".
Start-up and run-down parameters
 Run-in steps, parameters .
Step 9 = shortest run-in time, ... Step 1 = longest run-in time.
 Start-up time, parameter
t
. Can be set in 1-second steps. The adjustable time range depends on the set
speed.
It is only possible to set start-up times if these have been activated. See the chapter "Activating or
deactivating start-up and run-down times".
 Braking stages, parameter .
1-9 = Linear braking curve.
Step 9 = shortest run-out time, ... Step 1 = long run-out time, step 0 = non-braked run-out.
 Run-down time, parameter
t
. Can be set in 1-second steps. The adjustable time range depends on the
set speed.
It is only possible to set run-down times if these have been activated. See the chapter "Activating or
deactivating start-up and run-down times".
 Brake switch-off revolution, parameters N Brake.
Adjustable from 50 rpm to the maximal rotor speed (Nmax) in increments of 10.
Once the rotor speed has been reached, non-braked run-out begins.

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T/°C
 Temperature (only for centrifuge with refrigeration)
Adjustable in degrees Celsius (°C) or in degrees Fahrenheit (°F). For setting the temperature unit, see
chapter "Temperatur-Einheit einstellen/Setting the temperature unit".
Parameters T/°C = degrees Celsius (°C).
Adjustable from -20°C to +40°C, in 1°C increments (for the heat/cool option, adjustable in the range from -
20°C to +90°C).
Parameters T/°F = degrees Fahrenheit (°F).
Adjustable from 4°F to +104°F, in 1°F increments (for the heat/cool option, adjustable in the range from -
4°F to +194°F).
The lowest temperature that can be reached depends on the rotor (see chapter "Anhang/Appendix,
Rotoren und Zubehör/Rotors and accessories").
 Activate or deactivate the heater, parameter Heater on/off (only with heating/cooling option).
 Scroll backwards in the menus.
PROG
 Call up programs and program linkages; parameter RCL.
Programs: Program locations 1 to 99 (for centrifuge without cooling) and program locations 1 to 98 and
PREC (for centrifuge with cooling). Program linkages: Program locations A to Z.
 Save programs and program linkages; parameter STO.
99 programs can be saved (for centrifuges without refrigeration: program places 1 to 99, for centrifuges
with refrigeration: program places 1 to 98 and PREC). The program place PREC (PRECOOLING) is
reserved for the precooling program. Program place 0 is used for temporary storage for centrifuge data
from the last centrifugation run. No programs can be saved at this program place.
It is possible to save 25 program linkages (program locations A to Z; there is no program location J). A
program linkage can consist of 20 programs.
 Link programs; parameter EDIT.
 Call up the "Machine menu" (keep the key press for 8 seconds).
 Scroll forwards in the menus.
 Start centrifugation run for pre-cooling the rotor (only for centrifuges with cooling). The LED in the key is lit
during the centrifugation run, as long as the rotor is revolving.
The centrifugation run for pre-cooling the rotor is done automatically with the program PREC
(PRECOOLING).
START
 Start centrifugation run. The LED in the key is lit during the centrifugation run, as long as the rotor is
revolving.
 Short-time centrifugation.
The centrifugation run occurs while the key is kept pressed. The LED in the key is lit during the
centrifugation run, as long as the rotor is revolving.
 Saving entries and changes.
 In the "Machine Menu", call up the submenus.
STOP
OPEN
 End centrifugation run.
The rotor decelerates with the preset run-down parameters. The right-hand LED in the button lights up
until the rotor is stationary. Once the rotor is stationary the left-hand LED flashes in the button. Pressing
the button twice triggers the EMERGENCY STOP.
 Unlock the lid.
The left-hand LED in the button goes out.
 Exit the parameter input and the menus.
18 Enter centrifugation parameters
It is not possible to input the centrifugation parameters if a program lock has been set. The functions of the
various program locks are described in the chapter "Setting program locks".
If no key is pressed for 8 seconds long after the selection or during the input of parameters, the previous
values will be shown in the display. The input of parameter then has to be executed again.
If several parameters are input, the
START
key must be pressed after setting the last parameter in order to
accept all changes.
If parameters are changed, the program place number is displayed in parentheses (). This means that the
centrifugation data in the display no longer corresponds to the centrifugation data from the program place that
has been saved.
You can no discontinue entering parameters at any time by pressing the key
OPEN / STOP
. In this case the
adjustments are not saved.

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18.1 Running time
In order to set continual running, the minutes, seconds and hours have to be set to zero.
Continual running is represented in the display by the following symbol, "".
 Press the
TIME
key. The parameters t/hms is displayed. The minutes (m) are shown in parentheses , and can
be changed.
 Use the adjusting knob to set the value you want.
 Press the
TIME
key. The seconds (s) are shown in parentheses and can be changed.
 Use the adjusting knob to set the value you want.
 Press the
TIME
key. The hours (h) are shown in parentheses  and can be changed.
 Use the adjusting knob to set the value you want.
 To apply the setting to the display, either press the
START
key or press the
TIME
key as often as is required until
the centrifugation data are displayed.
18.2 Starting runtime timing
The start to runtime timing can only be set if the function, "Dual time mode" has been activated. See chapter
"Activate or deactivate function "Dual time mode". This function is activated ex works.
 Press the
TIME
key as often as is required until Timing begins at start or Timing begins at speed is displayed.
 Select the desired setting with the turning knob .
Timing begins at Start = The runtime begins to count immediately after the centrifugation run starts.
Timing begins at Speed =The runtime only begins to count after the set speed is reached.
This is indicated on the display by the symbol , to the left next to the time.
 Press the
TIME
or
START
keys to apply the setting to the display.
18.3 Speed (RPM)
 Press the
RPM
key. The parameters RPM is displayed.
 Use the adjusting knob to set the value you want.
 Press the
RPM
or
START
keys to apply the setting to the display
18.4 Relative centrifugal force (RCF) and centrifuging radius (RAD)
Relative centrifugal force (RCF) depends on the centrifugal radius (RAD). Before RCF is set, the centrifugal
radius has to be set.
 Press the
RCF
key as often as is required until the parameters RAD and RCF are displayed and the value of
the parameter, RAD is displayed in parentheses,  e.g. RAD = 146RCF = 3695. The LED is lit in the key.
 Use the adjusting knob to set the centrifuging radius you want.
By changing the centrifuging radius, the value adjusts automatically to the RCF.
 Press the
RCF
key again. The value of the parameter, RCF is displayed in  parentheses, e.g. RAD = 146
RCF = 3695.
 Use the adjusting knob to set the RCF you want.
 Press the
PROG
key to save the set RCF value. See the "Inputting or changing programs" chapter.
Only by storing (STO) the set RCF value will the RPM value resulting from that be accepted.