Hettich MIKRO 185 User manual

Rev. 02 / 11.2022 Andreas Hettich GmbH & Co. KG AB1203EN_SA
MIKRO 185
EN
Operating Manual............................................................ 3

2/38 Rev. 02 / 11.2022 AB1203EN_SA
Andreas Hettich GmbH & Co. KG
Föhrenstraße 12, D-78532 Tuttlingen / Germany
Phone [phone] / 705-0
Fax [phone]
[email protected], ser[email protected]
www.hettichlab.com
Single Registration Number: DE-MF-000010680
© 2015 by Andreas Hettich GmbH & Co. KG
All rights reserved. No part of this publication may be reproduced without the prior written permission of the copyright
owner.
Änderungen vorbehalten! , Modifications reserved! , Sous réserve de modifications ! , Con riserva di modifiche!
AB1203EN_SA / Rev. 02

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Contents
1How to use these operating instructions.................................................................................................................5
2Symbol meanings ...................................................................................................................................................5
3Intended use...........................................................................................................................................................6
4Remaining risks......................................................................................................................................................6
5Technical specifications..........................................................................................................................................7
6Notes on safety.......................................................................................................................................................8
7Transport and storage...........................................................................................................................................10
7.1 Transport.......................................................................................................................................................10
7.2 Storage..........................................................................................................................................................10
8Scope of delivery ..................................................................................................................................................10
9Removing the transport securing device...............................................................................................................11
10 Initial operation..................................................................................................................................................11
11 Opening and closing the lid...............................................................................................................................12
11.1 Opening the lid...........................................................................................................................................12
11.2 Closing the lid ............................................................................................................................................12
12 Installation and removal of the rotor..................................................................................................................12
13 Loading the rotor...............................................................................................................................................13
14 Closing biosafety systems.................................................................................................................................13
15 Operating and display elements........................................................................................................................14
15.1 Displayed symbols.....................................................................................................................................14
15.2 Control panel keys .....................................................................................................................................14
15.3 Setting options ...........................................................................................................................................15
16 Entering centrifugation parameters ...................................................................................................................16
16.1 Direct input of the centrifugation parameters .............................................................................................16
16.1.1 Speed (RPM)......................................................................................................................................16
16.1.2 Relative centrifugal force (RCF) and centrifugal radius (RAD)............................................................16
16.1.3 Runtime ..............................................................................................................................................16
16.2 Input of the centrifugation parameters with the "SELECT" key..................................................................17
17 Centrifugation....................................................................................................................................................19
17.1 Centrifugation with preset time...................................................................................................................19
17.2 Continuous run...........................................................................................................................................20
17.3 Short-term centrifugation............................................................................................................................21
18 Emergency Stop................................................................................................................................................21
19 Cycle counter ....................................................................................................................................................22
20 Settings and queries..........................................................................................................................................22
20.1 Querying system information .....................................................................................................................23
20.2 Acoustic signal...........................................................................................................................................25
20.3 Optical signal after ending the centrifugation run.......................................................................................26
20.4 Automatic unlocking of the lid after the centrifugation run..........................................................................27
20.5 Backlighting of the display..........................................................................................................................28
20.6 Querying the hours of operation and the number of centrifugation runs ....................................................29
20.7 Resetting the cycle counter to zero............................................................................................................30
21 Relative centrifugal force (RCF)........................................................................................................................31

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22 Centrifugation of materials or mixtures of materials with a density higher than 1.2 kg/dm3...............................31
23 Emergency unlocking........................................................................................................................................31
24 Maintenance and servicing................................................................................................................................32
24.1 Centrifuge (housing, lid and centrifuging chamber)....................................................................................32
24.1.1 Surface cleaning and care..................................................................................................................32
24.1.2 Surface disinfection ............................................................................................................................32
24.1.3 Removal of radioactive contaminants.................................................................................................32
24.2 Rotors and Attachments ............................................................................................................................33
24.2.1 Cleaning and care...............................................................................................................................33
24.2.2 Disinfection.........................................................................................................................................33
24.2.3 Removal of radioactive contaminants.................................................................................................33
24.2.4 Rotors and accessories with limited service lives...............................................................................33
24.3 Autoclaving ................................................................................................................................................34
24.4 Centrifuge containers.................................................................................................................................34
25 Faults ................................................................................................................................................................35
26 Change mains input fuses.................................................................................................................................36
27 Returning Devices.............................................................................................................................................36
28 Disposal ............................................................................................................................................................36
29 Anhang / Appendix............................................................................................................................................37
29.1 Rotoren und Zubehör / Rotors and accessories.........................................................................................37

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1 How to use these operating instructions
 Before using the centrifuge, read the operating instructions and observe them.
 The operating instructions are a part of the device. They must always be kept readily available.
 If the device is set up at a different location, the operating instructions must be provided with it.
2 Symbol meanings
Symbol on the device:
Attention, general hazard area.
Symbol on the device:
Observe operating instructions.
This symbol indicates that the user must observe the operating instructions provided.
Symbol in this document:
Attention, general hazard area.
This symbol refers to safety relevant warnings and indicates possibly dangerous situations.
The non-adherence to these warnings can lead to material damage and injury to personal.
Symbol on the device and in this document:
Beware of biohazard.
Symbol in this document:
This symbol refers to important circumstances.
Symbol on the device and in this document:
Symbol for the separate collection of electric and electronic devices according to the guideline
2002/96/EG (WEEE). The device belongs to Group 8 (medical devices).
Applies in the countries of the European Union, as well as in Norway and Switzerland.
Symbol on the shipping carton label:
This way up.
Symbol on the shipping carton label:
The shipping packaging must be transported and
handled within the indicated humidity range (10% - 80%).
Symbol on the shipping carton label:
The shipping packaging must be stored, transported and
handled within the temperature range shown (-20°C - +60°C).
Symbol on the shipping carton label:
The shipping packaging must be kept away from rain and
kept in a dry environment.
Symbol on the shipping carton label:
Fragile, handle with care.
Symbol on the shipping carton label:
Stack limit. Maximum number of identical packages which may be stacked on the bottom package,
whereby “n“ stands for the number of permissible packages. The bottom package is not included in “n”.

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3 Intended use
This device is a medical product (laboratory centrifuge) according to the in vitro diagnostic medical devices regulation
(EU) 2017/746.
The centrifuge was designed only for the separation of materials or mixtures with a density of no more than 1.2
kg/dm³.
This includes sample preparations for molecular biological investigations in the clinical area. Here, centrifugation is a
decisive step for extracting and purifying DNA and RNA from patient samples. In addition, many special kits are used
routinely. If kits and spin columns are used, the centrifuge, with a sufficiently high centrifugal acceleration and its
specially designed rotor for the centrifugation of spin column kits, meets the requirements for sample preparation in
infection diagnostics.
The centrifuge is only meant for this purpose.
Another use or one which goes beyond this, is considered to be non-intended. The company Andreas Hettich GmbH
& Co. KG is not liable for damage resulting from this.
Observing all information in the operating instructions and complying with the measures described therein is also a
part of the intended use.
If the centrifuge is installed in another device or in a system, the manufacturer of the overall system is responsible for
its safety.
4 Remaining risks
The device is built according to the state-of-the-art and the recognized safety regulations. If used and handled
improperly, there could be life-threatening danger to the user or third parties, or the device could be impaired or there
could be other property damage. The device is only to be used for its intended purpose and only when it is in safe
working condition.
Malfunctions which could affect safety must be corrected immediately.

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5 Technical specifications
Manufacturer Andreas Hettich GmbH & Co. KG
D-78532 Tuttlingen
Model MIKRO 185
Basic-UDI-DI 4050674010009QG
Type 1203 1203-01
Mains voltage (10%) 200 - 240 V 1100 - 127 V 1
Mains frequency 50 - 60 Hz 50 - 60 Hz
Connected load 390 VA 390 VA
Current consumption 1.8 A 3.6 A
Max. capacity 24 x 1.5 / 2.0 ml
Allowed density 1.2 kg/dm3
Speed (RPM) 14000
Force (RCF) 18845
Kinetic energy 2450 Nm
Obligatory inspection
(DGUV Regel 100 - 500) no
Ambient conditions (EN / IEC 61010-1)
 Set-up site Indoors only
 Altitude Up to 2000 m above sea level
 Ambient temperature 2°C to 40°C
 Humidity Maximum relative humidity 80% for temperatures up to 31°C, linearly
decreasing to 50% relative humidity at 40°C.
 Excess-voltage category
(IEC 60364-4-443) 
 Pollution degree 2
Device protection class 
Not suitable for use in explosion-endangered areas.
EMC
 Emitted interference,
Interference immunity EN / IEC 61326-1, Class B FCC Class B
Noise level (dependent on rotor) 59 dB(A)
Dimensions
 Width 261 mm
 Depth 353 mm
 Height 228 mm
Weight approx. 11 kg

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6 Notes on safety
No claim of warranty will be considered by the manufacturer unless ALL instructions in this manual
have been followed.
Reports of serious incidents involving the device
Report any serious incidents involving the device to the manufacturer and, if necessary, to the competent
authority.
 The centrifuge should be installed on a good, stable base.
 Before using the centrifuge absolutely check the rotor for firm placement.
 When the centrifuge is running, according to EN / IEC 61010-2-020, no persons, dangerous
substances or objects may be within the safety margin of 300 mm around the centrifuge.
 Rotors, suspensions and accessories that possess traces of corrosion or mechanical damage or
if their term of use has expired may not be used any longer.
 The centrifuge may no longer be put into operation when the centrifuging chamber has safety-
related damages.
 For centrifuges without temperature control, when the room temperature is increased and/or if the
device is frequently used, the centrifuging chamber could be heated up. Therefore, it can't be
ruled out that the sample material might be changed due to the temperature.
 Before the initial operation of your centrifuge you should read and pay attention to the operating
instructions. Only personnel that has read and understood the operating instructions are allowed to
operate the device.
 Along with the operating instructions and the legal regulations on accident prevention, you should also follow the
recognised professional regulations for working in a safe and professional manner. These operating instructions
should be read in conjunction with any other instructions concerning accident prevention and environmental
protection based on the national regulations of the country where the device is to be used.
 This centrifuge is a state-of-the-art piece of equipment which is extremely safe to operate. However, it can lead to
danger for users or others if used by untrained staff, in an inappropriate way or for a purpose other than that it
was designed for.
 The centrifuge must not be moved or knocked during operation.
 In case of fault or emergency release, never touch the rotor before it has stopped turning.
 To avoid damage due to condensate, when changing from a cold to a warm room the centrifuge must either heat
up for at least 3 hours in the warm room before being connected to the mains, or run hot for 30 minutes in the
cold room.
 Only the rotors and accessories approved by the manufacturer for this device may be used (see chapter
"Anhang/Appendix, Rotoren und Zubehör/Rotors and accessories"). Before centrifuge vessels are used which are
not listed in the chapter "Appendix, Rotors and accessories", the user must make sure they can be used by
asking the manufacturer.
 The centrifuge rotor may only be loaded in accordance with the chapter "Loading the rotor".
 When centrifuging with maxim revolutions per minute the density of the materials or the material mixtures may not
exceed 1.2 kg/dm3.
 The centrifuge may only be operated when the balance is within the bounds of acceptability.
 The centrifuge may not be operated in explosion-endangered areas.
 The centrifuge must not be used with:
 inflammable or explosive materials
 materials that react with one another producing a lot of energy.

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 If users have to centrifuge hazardous materials or compounds contaminated with toxic, radioactive or pathogenic
micro-organisms, they must take appropriate measures.
For hazardous substances centrifuge containers with special screw caps must strictly be used. In addition to the
screw cap centrifuge containers, for materials in hazard category 3 and 4 a biosafety system must be used (see
the World Health Organisation’s “Laboratory Biosafety Manual”).
In a biosafety system, droplets and aerosols are prevented from escaping by a bioseal (packing ring).
Damaged packing rings must not be used to seal the biosafety system.
Without the use of a biosafety system the centrifuge is not microbiologically sealed in the sense of the
EN / IEC 610101-2-020 standard.
When closing a biosafety system, please observe the instructions provided in the chapter, "Closing Biosafety
Systems".
For further details of available biosafety systems see chapter "Anhang/Appendix, Rotoren und Zubehör/Rotors
and accessories". If in doubt, you should obtain relevant information from the manufacturer.
 The centrifuge must not be operated with highly corrosive substances which could impair the mechanical integrity
of rotors, hangers and accessories.
 Repairs must only be carried out by personnel authorised to do so by the manufacturer.
 Only original spare parts and original accessories licensed by the Andreas Hettich GmbH & Co. KG company are
allowed to be utilised.
 The following safety regulations apply:
EN / IEC 61010-1 and EN / IEC 61010-2-020 as well as their national deviations.
 The safe operation and reliability of the centrifuge can only be guaranteed if:
 the centrifuge is operated in accordance with the operating instructions,
 the electrical installation on the site where the centrifuge is installed conforms to the demands of EN / IEC
stipulations.
 Meeting the country-specific requirements concerning occupational safety with regard to the use of laboratory
centrifuges at the workplaces provided for this purpose by the user is the responsibility of the user.

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7 Transport and storage
7.1 Transport
Before transporting the device, the transport securing device must be installed.
When the device and accessories are transported, the following ambient conditions must be complied with:
 Ambient temperature: -20°C to +60°C
 Relative humidity: 10% to 80%, non-condensing
7.2 Storage
The device and the accessories may only be stored in closed and dry rooms.
When the device and accessories are stored, the following ambient conditions must be complied with:
 Ambient temperature: -20°C to +60°C
 Relative humidity: 10% to 80%, non-condensing
8 Scope of delivery
The following accessories are delivered with the centrifuge:
1 Power cord
2 fuses
1 hex head wrench
1 operating manual
1 information sheet, transport securing device
1 information sheet, emergency unlocking
The rotor(s) and associated accessories are included in the delivery in the quantity ordered.

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9 Removing the transport securing device
It is imperative that the transport securing device be removed.
Keep the transport securing device in a safe place since it must be installed again before transporting the
device.
The device may only be transported with the transport securing device installed.
To protect the device from damage during transport, the motor is fixed in place.
This transport securing device must be removed when the device is put into operation.
(a)
(b) (b)
(a)
 Remove the two screws (a) and spacer sleeves (b).
The transport securing device is installed in the opposite order.
10 Initial operation
 A type B residual current circuit breaker must be used if the device is additionally protected with a residual
current circuit breaker in the building installation. When using a different type, the residual current circuit breaker
may either not switch off the unit if there is a fault on the unit, or it may switch off the unit even though there is no
fault on the unit.
 Remove the transport securing device from the housing floor; see the "Removing the transport securing device"
chapter.
 Position the centrifuge in a stable and level manner in a suitable place. During set-up, the required safety
margin of 300 mm around the centrifuge is to be kept according to EN / IEC 61010-2-020.
When the centrifuge is running, according to EN / IEC 61010-2-020, no persons, dangerous
substances or objects may be within the safety margin of 300 mm around the centrifuge.
 Ventilation openings may not be blocked.
A distance of 300 mm must be maintained from the ventilation slots and openings of the centrifuge.
 Check whether the mains voltage tallies with the statement on the type plate.
 Connect the centrifuge with the power cord to a standard mains socket. For connection ratings refer to Chapter
"Technical specifications".
 Switch on the mains switch (switch position "").
The following displays appear one after the other:
1. The centrifuge model
2. The type number and program version
3. The last used centrifugation data
If the lid is closed, the message "Open the lid" is displayed.
In this case, open the lid to display the centrifugation data.

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11 Opening and closing the lid
11.1 Opening the lid
The lid can only be opened if the centrifuge is switched on and the rotor is stationary.
If this shouldn't be possible, see the "Emergency unlock" chapter.
When the cycle counter is activated, after a centrifugation run, while opening the lid, the remaining number
of running cycles (centrifugation runs) is briefly displayed.
Example:
t/min:s RPM >RCF<
STOP
OPEN
 Press the key.
The lid is unlocked by the motor.
: Lid unlocked.
Example:
t/min:s RPM >RCF<
11.2 Closing the lid
Do not reach with your fingers between the lid and housing.
Do not slam the lid closed.
 Put the lid on and lightly press the front
edge of the lid down.
The lid is locked by the motor.
: Lid locked.
Example:
t/min:s RPM >RCF<
12 Installation and removal of the rotor
C
D
A
B
Installation:
 Clean the motor shaft (A) and the bore of the rotor and then apply a
thin coat of grease to the motor shaft. Dirt particles between the
motor shaft and rotor prevent the rotor from having a perfect seat and
cause it to run unsteadily.
 Place the rotor vertically onto the motor shaft. The motor shaft dog
(B) has to fit in the rotor slot (C). The alignment of the groove is
labelled on the rotor (D).
 Tighten the clamping nut of the rotor with the Allen wrench (included
in delivery) by turning clockwise.
 Check the rotor to make sure it is seated firmly.
To ensure a tight fit of the rotor, the nut of the rotor must
be hand-tightened.
Removal:
 Loosen the rotor's clamping nut by turning counter-clockwise with the
Allen wrench (included in delivery) and turn up to the lifting pressure
point. After overcoming the lifting pressure point, the rotor is released
from the cone of the motor shaft. Turn the clamping nut until the rotor
can be lifted up from the motor shaft.
 Lift up the rotor from the motor shaft.

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13 Loading the rotor
Standard centrifuge containers of glass will not stand RCF values exceeding 4000 (DIN 58970, pg. 2).
 Check the rotor for firm seating.
 The rotors must be loaded symmetrically. The centrifuge containers have to be distributed evenly on all rotor
positions. For authorised combinations see Chapter "Anhang/Appendix, Rotoren und Zubehör/Rotors and
accessories".
Example:
Rotor is evenly loaded Not permitted!
Rotor is not evenly loaded
 The centrifuge containers may only be filled outside of the centrifuge.
 The maximum filling quantity for the centrifuge containers specified by the manufacturer must not be exceeded.
The centrifuging vessels may only be filled so
far that no fluid can be expelled from them
while the centrifuge is running.
Fluid
Centrifugal force
 When loading the rotor, no liquid may enter the rotor or the centrifuging chamber.
 In order to maintain the weight differences within the centrifuge container as marginal as possible, a consistent
fill level in the containers is to be heeded.
 The weight of the permissible filling quantity is specified on each rotor. This weight may not be exceeded.
14 Closing biosafety systems
In order to guarantee that it is sealed, the lid to the biosafety system has to be firmly closed.
In order to prevent the packing ring from twisting when opening and closing the cover, the packing ring must
be lightly rubbed with a rubber care product.
For further details of available biosafety systems see chapter "Anhang/Appendix, Rotoren und Zubehör/Rotors
and accessories". If in doubt, you should obtain relevant information from the manufacturer.
Lid with screw closure and turning handle
 Place the lid centred on the rotor.
 Tightly seal the lid by hand by turning the turning handle clockwise.

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15 Operating and display elements
SELECT
t/min:s
RPM
>RCF<
RPM/RCF
t
RCF
STOP
OPEN
START
PULSE
15.1 Displayed symbols
Lid unlocked.
Lid locked.
Rotation display. The rotation display lights up, rotating counterclockwise, as long as the rotor is turning.
15.2 Control panel keys
RPM/RCF
 Input the speed directly.
If the key is kept pressed, the value changes with increasing speed.
t
 Input the runtime directly.
Adjustable in steps of 1 second up to a minute, and in steps of 1 minute starting from 1 minute.
 Input the centrifuging parameters.
If the key is kept pressed, the value changes with increasing speed.
SELECT
 Selection key for activating the individual parameters.
Every time the key is pressed, the next parameter is activated.
 Call up the "MACHINE MENU" (keep the key pressed for 8 seconds).
 In the "Machine Menu", select the menus " Info", " Settings" and " Time & Cycles".
 Scroll forward in the menus.
RCF
 Switching between the RPM display (RPM) and RCF display ( RCF ).
RCF values are displayed in parentheses .
RPM Speed
RCF : Relative centrifugal acceleration
START
PULSE
 Start the centrifugation run.
 Short-term centrifugation.
Centrifugation is run as long as the key is kept pressed.
 Call up the menus " Info", " Settings" and " Time & Cycles".
STOP
OPEN
 Finish the centrifugation run.
The rotor runs down with a pre-selected brake stage.
Pressing the key twice triggers the Emergency Stop.
 Unlock the lid.

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15.3 Setting options
t/min Runtime. Adjustable from 1 - 99 min, in steps of 1 minute.
t/sec Runtime. Adjustable from 1 - 59 s, in steps of 1 second.
Continuous run "--:--". Set the parameters t/min and t/sec to zero.
RPM Speed
A number value from 200 rpm to the maximum speed of the rotor can be set.
Adjustable from 200 RPM to 10000 RPM in steps of 10, and above 10000 RPM, in steps of 100.
For the maximum rotor speed, see the chapter "Appendix, Rotors and accessories".
RCF Relative centrifugal force
A number value can be set which results in a speed between 200 rpm and the maximum rotor speed.
Adjustable up to 10000 in steps of 1, and above 10000, in steps of 10.
It is only possible to input the relative centrifugal force (RCF) if the RCF display ( RCF ) is
activated.
The relative centrifugal force (RCF) depends on the centrifuging radius (RAD). After entering the
RCF, check to make sure that the correct centrifuging radius has been set.
RAD/mm Centrifuging radius
Adjustable from 10 mm to 250 mm, in steps of 1 mm.
For the centrifuging radius, see the chapter "Appendix, Rotors and accessories".
It is only possible to input the centrifuging radius if the RCF display ( RCF ) is activated.
DEC Brake stage. fast = short run-out time, slow = long run-out time.

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16 Entering centrifugation parameters
16.1 Direct input of the centrifugation parameters
The speed (RPM), the relative centrifugal force (RCF), the centrifuging radius (RAD) and the runtime can be input
directly with the keys without previously having to press the
SELECT
key.
The set centrifugation parameters are only stored after starting the centrifugation run.
16.1.1 Speed (RPM)
Example:
RCF
 Press the key to activate the RPM display
(RPM) as needed.
t/min:s RPM >RCF<

RPM/RCF
 Set the desired value with the keys.
t/min:s RPM >RCF<
16.1.2 Relative centrifugal force (RCF) and centrifugal radius (RAD)
Example:
RCF
 Press the key to activate the RCF display
(RCF ) as needed.
t/min:s RPM >RCF<

RPM/RCF
 Set the desired RCF value with the keys.
t/min:s RPM >RCF<

t
 Set the desired centrifuging radius with
the keys as needed.
t/min:s RPM >RCF<
t/min:s RPM >RCF<
16.1.3 Runtime
Up to 1 minute, the runtime can be set in steps of 1 second, and starting from 1 minute, it can only be set in
steps of 1 minute.
In order to set the continuous run, the parameters t/min and t/sec must be set to zero. In the time display
(t/min:s), "--:--" appears.
Example:
t/min:s RPM >RCF<
Example:
t
 Set the desired value with the keys.
t/min:s RPM >RCF<

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16.2 Input of the centrifugation parameters with the "SELECT" key
The runtime can be set in minutes and seconds (parameters t/min and t/sec).
In order to set the continuous run, the parameters t/min and t/sec must be set to zero. In the time display
(t/min:s), "--:--" appears.
Example:
t/min:s RPM >RCF<
The relative centrifugal acceleration (RCF) depends on the centrifuging radius (RAD).
During the input of the RCF, the set centrifuging radius is displayed.
If no key is pressed for 8 seconds after selection or during parameter input, the previous values are shown on
the display. The parameters must then be input again.
By pressing the
START
PULSE
button, the settings will be saved.
If several parameters are input, the
START
PULSE
key must be pressed after setting the last parameter.
Entering parameters can be cancelled at any time by pressing the
STOP
OPEN
key. In this case, the settings are not
stored.
Example:
RPM display (RPM) Example: RCF display ( RCF )
RCF
 Press the key to activate the RPM display
(RPM) or the RCF display ( RCF ) as
needed.
t/min:s RPM >RCF< t/min:s RPM >RCF<

SELECT
 Press the key.
t/min : Runtime, minutes.
t/min:s RPM >RCF< t/min:s RPM >RCF<

t
 Set the desired value with the keys.
t/min:s RPM >RCF< t/min:s RPM >RCF<

SELECT
 Press the key.
t/sec : Runtime, seconds.
t/min:s RPM >RCF< t/min:s RPM >RCF<

t
 Set the desired value with the keys.
t/min:s RPM >RCF< t/min:s RPM >RCF<

Continued on next page

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Example:
RPM display (RPM) Example: RCF display ( RCF )
SELECT
 Press the key.
RPM : Speed.
RAD/mm : Centrifuging radius.
It is only possible to display and input the
centrifuging radius if the RCF display
(RCF ) is activated.
t/min:s RPM >RCF< t/min:s RPM >RCF<

t
 Set the desired value with the keys.
t/min:s RPM >RCF< t/min:s RPM >RCF<

SELECT
 Press the key.
R : Centrifuging radius.
RCF : Relative centrifugal force
t/min:s
RPM
>RCF<

t
 Set the desired value with the keys.
t/min:s
RPM
>RCF<

SELECT
 Press the key.
DEC : Brake stage.
fast : short run-out time.
slow : long run-out time.
t/min:s RPM >RCF< t/min:s RPM >RCF<

t
 Set the desired value with the keys.
t/min:s RPM >RCF< t/min:s RPM >RCF<

START
PULSE
 Press the button to save the settings.
t/min:s RPM >RCF<
t/min:s
RPM
>RCF<

EN
AB1203EN_SA Rev. 02 / 11.2022 19/38
17 Centrifugation
In accordance with EN / IEC 61010-2-020, no persons, hazardous substances or objects may be within a
safety range 300 mm around the centrifuge during a centrifugation run.
After each centrifugation run, while the lid is unlocking, the remaining number of running cycles (centrifugation
runs) will be briefly displayed.
Example:
t/min:s RPM >RCF<
If the permissible weight difference was exceeded within the rotor load, the centrifugation run is aborted during
start-up and the following will appear on the display:
t/min:s RPM >RCF<
A centrifugation run can be aborted at any time by pressing the
STOP
OPEN
key.
During a centrifugation run, centrifugation parameters can be selected and changed. The changed values,
however, only apply to the current centrifugation run and are not saved.
With the
RCF
key, you can toggle between the RPM display (RPM) and RCF display ( RCF ) at any time. If
the RCF display ( RCF ) is worked with, the centrifuging radius must be input.
The following is displayed:
t/min:s RPM >RCF<
Further operation of the centrifuge is then only possible after opening the lid.
Operation errors and malfunctions will be shown (see the chapter on “Malfunctions”).
 Switch on in the mains switch. Switch position .
 Load the rotor and close the centrifuge lid.
17.1 Centrifugation with preset time
Example:
RPM display (RPM) Example: RCF display ( RCF )
RCF
 Press the key to activate the RPM display
(RPM) or the RCF display ( RCF ) as
needed.
t/min:s RPM >RCF< t/min:s RPM >RCF<

 Enter the desired centrifugation parameters (see the
chapter “Entering centrifugation parameters”).
t/min:s RPM >RCF< t/min:s RPM >RCF<

START
PULSE
 Press the key to start the centrifugation
run.
During the centrifugation run, the rotor
speed or the resulting RCF value and
remaining time are displayed.
t/min:s RPM >RCF< t/min:s RPM >RCF<

Continued on next page

EN
20/38 Rev. 02 / 11.2022 AB1203EN_SA
Example:
RPM display (RPM) Example: RCF display ( RCF )
 After the time elapses or if the centrifugation run is
aborted by pressing the
STOP
OPEN
key, the run-out
occurs with the set brake stage.
The brake stage is displayed. Example f
t/min:s RPM >RCF< t/min:s RPM >RCF<
17.2 Continuous run
Example:
RPM display (RPM) Example: RCF display ( RCF )
RCF
 Press the key to activate the RPM display
(RPM) or the RCF display ( RCF ) as
needed.
t/min:s RPM >RCF< t/min:s RPM >RCF<

 Input the desired centrifugation parameters. Set the
parameters t/min and t/sec to zero (see the chapter
“Entering centrifugation parameters”).
t/min:s RPM >RCF< t/min:s RPM >RCF<

START
PULSE
 Press the key to start the centrifugation
run.
During the centrifugation run, the rotor
speed or the resulting RCF value and
elapsed time are displayed.
t/min:s RPM >RCF< t/min:s RPM >RCF<

STOP
OPEN
 Press the key to end the centrifugation
run.
The run-out occurs with the set brake
stage. The brake stage is displayed.
Example f
t/min:s RPM >RCF< t/min:s RPM >RCF<