Hitachi L-2200 User manual

INSTRUCTION MANUAL
OPTIONAL SYRINGE
FOR
MODEL L-2200/2200U AUTOSAMPLER
Hitachi High-Technologies Corporation
12th Edition, August 2009
Copyright C Hitachi High-Technologies Corporation 2003. 1st Edition, 2003
All rights reserved. Printed in Japan. Part No. 890-9203-11 TK-Y (SY-HMS)

Be sure to read through and understand the following
points with regard to this manual.
1. Information contained in this document is subject to
change without notice for improvement.
2. This manual is copyrighted by Hitachi High-
Technologies Corporation with all rights reserved.
No part of this manual may be reproduced,
transmitted or disclosed to a third party in any form
or by any means without the express written
permission of Hitachi High-Technologies
Corporation.
3. Hitachi High-Technologies Corporation assumes no
liability for any direct, indirect, or consequential
damages arising from use not described in this
manual.
Utmost care must be exercised when using the
instrument.
4. This document does not provide any warranty or
permission for industrial properties or any rights to
grant license lawfully and without infringement.

PREFACE
Thank you very much for purchasing the optional syringe to be
used on Hitachi Model L-2200/2200U autosampler.
This optional syringe is specifically designed for the L-2200/
2200U autosampler. It provides a wide range of sample
injection from 50 µL to 4500 µL.
Note that physiologically or biologically active samples are not
applicable to this syringe because of possible infection with
bacteria or viruses.
This product is intended for use by persons having a basic
knowledge of chemical analysis, and is designed for general
analytical purposes only. Remember that improper use of
analytical instruments, chemicals or samples would result
not only in wrong analytical data but also in consequences
adverse to safety. Note that it is allowed only for persons
having a basic knowledge of chemical analysis procedures
to use this instrument.
Carefully read this instruction manual before attempting
operation. For proper use of this product, please acquaint
yourself with it.
After reading this manual, keep it in a safe place nearby so it can
be referred to whenever needed.
ABOUT THIS MANUAL
The operating procedures and maintenance/checkup instructions
for the L-2200/2200U autosampler optional syringe are
described in this manual.
First of all, read “IMPORTANT” at the beginning of this
manual and “SAFETY SUMMARY” contained in the L-2200/
2200U Autosampler Instruction Manual.
For proper use of the optional syringe, read through the L-2200/
2200U Autosampler Instruction Manual also.
If installation of this product is not yet completed, refer to
Section 2.

IMPORTANT - 1
IMPORTANT
WARRANTY ON PRODUCT
The Model L-2200/2200U Autosampler Optional Syringe is
warranted to be free from defects in material or workmanship
under normal use within the product specifications indicated in
this manual and under conditions given below. This warranty is
void if the instrument is not used according to the instruction
manual.
(1) Scope of Warranty
Any parts that prove to be defective in design or
workmanship during the warranty period will be repaired,
adjusted or replaced without charge. A substitute part
may be used for repair, or replacement with an equivalent
product may be made instead of repair. Such system
components as a personal computer and printer to be
updated frequently for improvement may not be available in
original versions at the time of replacement. Note that this
warranty does not apply to the instrument after it is
discarded, or if modified by the user or resold without
permission from the manufacturer, consumable parts, and
any failure of lifetime-expired parts.
The manufacturer assumes no liability for any damage to
data or application software due to any possible fault or
failure of this product.
(2) Warranty Period
One year from the date of initial installation
(In case a separate warranty document has been issued,
the warranty period indicated in it takes precedence over
the above period.)
(3) Limitations and Exclusions on Warranty
Note that the following cases are excluded from the scope
of this warranty, i.e., these cases are beyond the coverage
of free-of-charge repair even during the warranty period
indicated above.
(a) Failure due to operation at a place not meeting the
installation requirements specified by the manufacturer.

IMPORTANT - 2
(b) Failure due to power supply voltage/frequency other
than specified by the manufacturer or due to
abnormality in power supply.
(c) Corrosion or deterioration of the tubing due to
impurities contained in gas, air or cooling water
supplied by the user.
(d) Corrosion of the electric circuits or deterioration of the
optical elements due to highly corrosive atmospheric
gas.
(e) Failure due to use of hardware, software or spare parts
other than specified by the manufacturer.
(f) Failure due to improper handling not described in the
instruction manual or repair not approved by the
manufacturer.
(g) Failure due to maintenance or repair by a service
agent not approved or authorized by the manufacturer.
(h) Failure due to relocation or transport not approved by
the manufacturer after initial installation.
(i) Failure due to disassembly, modification or relocation
not approved by the manufacturer.
(j) Failure due to acts of God, including fire, earthquake,
storm, flood, lightning, social disturbance, riot, crime,
insurrection, war (declared or undeclared), radioactive
pollution, contamination with harmful substance, etc.
(k) Failure of the hardware, or damage to the system
software, application software, data or hard disk due to
computer virus infection.
(l) After disposal of this instrument, after its resale without
prior approval from the manufacturer, consumable
parts, and failure of any part that have reached the end
of its service life.
(m) Failure due to a life-limited part that has exceeded the
end of its useful lifetime.

IMPORTANT - 3
(4) Disclaimer of Warranty
THE MANUFACTURER MAKES NO WARRANTIES,
EITHER EXPRESS OR IMPLIED, EXCEPT AS PROVIDED
HEREIN, INCLUDING WITHOUT LIMITATION THEREOF,
WARRANTIES AS TO MARKETABILITY,
MERCHANTABILITY, FOR A PARTICULAR PURPOSE
OR USE, OR AGAINST INFRINGEMENT OF ANY
PATENT. IN NO EVENT SHALL THE MANUFACTURER
BE LIABLE FOR ANY DIRECT, INCIDENTAL OR
CONSEQUENTIAL DAMAGES OF ANY NATURE, OR
LOSSES OR EXPENSES RESULTING FROM ANY
DEFECTIVE PRODUCT OR THE USE OF ANY PRODUCT.
NO ORAL OR WRITTEN INFORMATION OR ADVICE
GIVEN BY THE MANUFACTURER, ITS DEALERS,
DISTRIBUTORS, AGENTS OR EMPLOYEES SHALL
CREATE A WARRANTY OR IN ANY WAY INCREASE
THE SCOPE OF THIS WARRANTY.

IMPORTANT - 4
INSTALLATION, RELOCATION AND AFTER-SALE TECHNICAL SERVICE
(1) Installation and Relocation
(a) It is not allowed for the user to perform installation at
the time of delivery of the instrument.
For ensuring safety and high accuracy of the
instrument, installation shall be carried out by or under
supervision of qualified service personnel of the
manufacturer or its authorized service agent.
(b) Before installation of the instrument, the customer is
requested to make necessary preparation referring to
this manual.
(c) If relocation of the instrument becomes necessary
after initial installation (delivery), please notify your
local Hitachi High-Technologies Corporation sales
representative or service office of Hitachi High-
Technologies Corporation sales representative.
(2) After-sale Technical Service
(a) For details of after-sale technical service, contact your
local Hitachi High-Technologies Corporation sales
representative or service office of Hitachi High-
Technologies Corporation sales representative.
(b) For service after the warranty period, consult your local
Hitachi High-Technologies Corporation sales
representative or service office of Hitachi High-
Technologies Corporation sales representative with
regard to a maintenance and inspection service
contract.

IMPORTANT - 5
TECHNICAL SEMINARS AND TRAINING FOR CUSTOMERS
For the customers to acquire in-depth understanding of the
analytical instruments, technical seminars and customer training
courses are available at the manufacturer or your site.
For further information, contact your local Hitachi High-
Technologies Corporation sales representative.
(The technical seminars and customer training courses are
available on a chargeable basis.)
DISPOSAL OF INSTRUMENT
Although at present this instrument does not use harmful
materials that would directly cause environmental disruption, the
environmental protection laws and regulations may be revised or
amended. When disposing of this instrument, be sure to check
the latest issues of the relevant laws and regulations or consult
your local Hitachi High-Technologies Corporation sales
representative.
SERVICE LIFE OF THIS INSTRUMENT
This instrument has a useful service life of seven years after the
date of its initial use (installation), which is estimated under the
condition that periodic maintenance, checkup, replacement of
life-limited parts, and repair of worn parts are carried out as
specified in the present instruction manual.
(In use of the instrument under standard operating conditions)

IMPORTANT - 6
OTHER PRECAUTIONS
Handling of Chemicals and Samples
(1) The user is responsible for following relevant legal
standards and regulations in the handling, storage and
discarding of chemicals and samples used in analytical
operations of the instrument.
(2) Reagents, standard solutions and accuracy-control
samples shall be handled, stored and discarded as
instructed by the respective suppliers.
(3) Physiologically or biologically active samples are not
applicable to this syringe because of possible infection with
bacteria or viruses.

- i -
CONTENTS
PREFACE
ABOUT THIS MANUAL
IMPORTANT ...............................................................................................IMPORTANT-1
WARRANTY ON PRODUCT ..................................................................IMPORTANT-1
INSTALLATION, RELOCATION AND AFTER-SALE
TECHNICAL SERVICE...........................................................................IMPORTANT-4
TECHNICAL SEMINARS AND TRAINING FOR CUSTOMERS..............IMPORTANT-5
DISPOSAL OF INSTRUMENT................................................................IMPORTANT-5
SERVICE LIFE OF THIS INSTRUMENT.................................................IMPORTANT-5
OTHER PRECAUTIONS.........................................................................IMPORTANT-6
1. OUTLINE................................................................................................................ 1-1
1.1 Optional Syringe....................................................1-1
1.2 Syringe-Piping Combination................................. 1-2
2. INSTALLATION......................................................................................................2-1
2.1 Unpacking..............................................................2-1
2.2 Setup ...................................................................... 2-1
2.2.1 How to Set Syringe Capacity and
Wash Count Parameters............................. 2-1
2.2.2 How to Remove Parts ................................. 2-2
2.2.3 How to Mount 0.5 mL/1 mL/
2.5 mL Syringe............................................ 2-4
2.2.4 How to Mount 5 mL Syringe........................ 2-7
2.2.5 Check of Pipe in Motion .............................2-8
2.2.6 Replacement Parts for Use at
High Flow Rate............................................ 2-9
2.2.7 Additional Replacement Parts in
L-2200U...................................................... 2-9
3. FUNCTIONS........................................................................................................... 3-1
3.1 Control Knobs and Switches................................ 3-1
3.2 Intended Use.......................................................... 3-1
3.3 Specifications........................................................ 3-1
4. OPERATION ..........................................................................................................4-1
4.1 Preparation for Operation..................................... 4-1
4.2 Measurement ......................................................... 4-1
5. REPLACEMENT PARTS........................................................................................ 5-1
5.1 Consumables.........................................................5-1

1.1
1 - 1
1. OUTLINE
1.1 Optional Syringe
This product is an optional device specifically designed for Model
L-2200 autosampler. It provides a wide range of sample
injection from 50 µL to 4500 µL. For details of the L-
2200/2200U autosampler, refer to its instruction manual.
Table 1-1 lists optional syringe kits for the L-2200/2200U
autosampler.
Table 1-1 Optional Syringe Kits
Part No. Name Remarks
890-3511 0.5 mL syringe kit Syringe, Piping
890-3512 1.0 mL syringe kit Syringe, Piping
890-3513 2.5 mL syringe kit Syringe, Piping
890-3514 5.0 mL syringe kit Syringe, Piping

1.2 Syringe-Piping Combination
1 - 2
1.2 Syringe-Piping Combination
Five kinds of syringes are available as shown in Table 1-2.
For each syringe capacity, a sample loop for “needle - injection
valve connection” and a connecting coil for “injection valve -
syringe valve connection” are provided (see Figures 1-1 to 1-3).
Be sure to use any one of the syringe-piping combinations
indicated in Table 1-2.
To install an optional syringe onto the L-2200U autosampler,
remove the stainless pipe (P/N 890-3452, 0.1 mm ID ×0.36 m)
connected between the injection port and the injection valve port
3 on the L-2200U autosampler, and instead of it, connect the
stainless pipe (P/N 890-3451, 0.17 mm ID ×0.36 m) included in
the accessory box accompanying the L-2200 autosampler.
In an application where a considerably high flow rate is used
(e.g., a flow rate of 6 mL/mm or higher), replace the stainless
pipe connected between the injection port and the injection valve
port 3 with the stainless pipe (P/N 890-3473, 0.5 mm ID ×
0.36 m) furnished together with the optional syringe.
Table 1-2 Kinds of Syringes
Sample Loop
(For connection between needle
and port 6 of injection valve)
Connecting Coil
(For connection between port 5
of injection valve and port 3 of
syringe valve)
Syringe
Capacity
Syringe-
Piping
Combina-
tion Part
No.
Syringe
Part No.
Part No. Dimensions
(Capacity) Part No. Dimensions (Capacity)
0.1 mL* ⎯890-3228 890-3462 0.5 mm ID ×0.7 m
(0.14 mL) 890-3455 0.25 mm ID ×1.0 m (0.05 mL)
890-3481 0.5 mm ID ×0.7 m
(0.14 mL) [Loop 1]
0.5 mL 890-3511 080-1064
890-3466 0.8 mm ID ×0.8 m
(0.4 mL) [Loop 2]
890-3467 0.8 mm ID ×1.0 m
(0.5 mL)
890-3481 0.5 mm ID ×0.7 m
(0.14 mL) [Loop 1]
1 mL 890-3512 080-1592
890-3468 1.0 mm ID ×1.2 m
(0.9 mL) [Loop 2]
890-3469 1.0 mm ID ×1.3 m
(1.0 mL)
890-3481 0.5 mm ID ×0.7 m
(0.14 mL) [Loop 1]
2.5 mL 890-3513 080-1092
890-3470 1.0 mm ID ×3.1 m
(2.4 mL) [Loop 2]
890-3471 1.0 mm ID ×3.2 m
(2.5 mL)
5 mL 890-3514 080-1593 890-3464 1.0 mm ID ×6.4 m
(5.0 mL) 890-3465 1.0 mm ID ×6.4 m (5.0 mL)
* Furnished as a standard syringe kit for the L-2200 autosampler.

1.2
1 - 3
Fig. 1-1 Piping Arrangement (For 0.1 mL Syringe)
Fig. 1-2 Piping Arrangement (For 0.5 mL, 1 mL or 2.5 mL Syringe)
From Pump To Column
12
3
45
6
Injection valve
Stainless steel pipe
(890-3454)
(1.0 mm ID ×0.1 m)
Connected
t
o por
t
2 of syringe valve
Connected to por
t
3 of syringe valve
Connecting coil
(See Table 1-2)
Sample loop 1 (890-3481)
(0.5 mm ID ×0.7 m)
Stainless steel pipe
(890-3451, 0.17 mm ID ×0.36 m
or 890-3473, 0.5 mm ID ×0.36 m)
Needle
Injection por
t
Sample loop 2
(See Table 1-2)
Join
t
From Pump To Column
2
3
45
6
Injection valve
Stainless steel pipe
(890-3454)
(1.0 mm ID ×0.1 m)
Connected to por
t
2 of syringe valve
Connected to por
t
3 of syringe valve
Connecting coil
(890-3455)
(0.25 mm ID ×1.0 m)
Sample loop
(890-3462)
(0.5 mm ID ×0.7 m)
Stainless steel pipe
(890-3451)
(0.17 mm ID ×0.36 m)
Needle
Injection por
t
1

1.2 Syringe-Piping Combination
1 - 4
NOTICE: Stainless steel pipe (P/N 890-3451, 0.17 mm ID ×
0.36 m) and stainless steel pipe (P/N 890-3452,
0.1 mm ID × 0.36 m) can be exchanged each
other depending on a flow during analysis (see
2.2.6).
Fig. 1-3 Piping Arrangement (For 5 mL Syringe)
NOTICE: Stainless steel pipe (P/N 890-3451, 0.17 mm ID ×
0.36 m) and stainless steel pipe (P/N 890-3452,
0.1 mm ID × 0.36 m) can be exchanged each
other depending on a flow during analysis (see
2.2.6).
From Pump To Column
12
3
45
6
Injection valve
Stainless steel pipe
(890-3454)
(1.0 mm ID ×0.1 m)
Connected to port
2 of syringe valve
Connected to port
3 of syringe valve
Connecting coil
(890-3465)
(1.0 mm ID ×6.4 m)
Sample loop (890-3464)
(1.0 mm ID ×6.4 m)
Stainless steel pipe
(890-3451, 0.17 mm ID ×0.36 m
or 890-3473, 0.5 mm ID ×0.36 m)
Needle
Injection port

2.2.1
2 - 1
2. INSTALLATION
2.1 Unpacking
NOTICE: It is not allowed for the user to perform installation
at the time of delivery of the instrument.
For ensuring safety and high accuracy of the
instrument, installation shall be carried out by or
under supervision of qualified service personnel of
the manufacturer or its authorized service agent.
Before installation of the instrument, the customer
is requested to make necessary preparation
referring to this manual. If relocation of the
instrument becomes necessary after initial
installation (delivery), please notify your local
Hitachi High-Technologies Corporation sales
representative or service office of Hitachi High-
Technologies Corporation sales representative.
2.2 Setup Install this optional syringe in the inside of the L-2200/2200U
autosampler in the manner instructed below.
2.2.1 How to Set
Syringe Capacity
and Wash Count
Parameters
(1) Referring to the L-2200 Autosampler Instruction Manual -
4.3.2 (3) “Setting Operating Conditions” - (a) “Sequential
Parameters” (L-2200U Autosampler Instruction Manual -
4.3.2 (3) “Setting Operation Conditions” - (a) “Sequential
Parameters”), enter a code corresponding to the capacity
of the optional syringe to be used (SYRINGE VOL).
(2) Referring to the L-2200 Autosampler Instruction Manual -
4.3.2 (3) “Setting Operating Conditions” - (c) “WASH
PARAM” (L-2200U Autosampler Instruction Manual - 4.3.2
(3) “Setting Operation Conditions” - (c) “WASH PARAM”),
specify “1” for INJ WASH STROKES that is a wash
condition setting parameter in the sequential program to
be carried out.

2.2 Setup
2 - 2
(3) Turn off power to the instrument.
NOTICE: A settable syringe speed varies depending on the
capacity of the syringe to be used (SYRINGE
VOL).
In the use of the optional syringe, the needle can
be washed efficiently even under the condition that
“1” is specified for INJ WASH STROKES.
2.2.2 How to Remove
Parts
Remove the following parts (see Figures 1-1, 2-1, 2-2 and 2-3).
(1) Using the spanner furnished together with the L-2200/
2200U autosampler, remove the sample loop (890-3462)
and connecting coil (890-3455).
(2) Remove the 0.1 mL syringe.
(3) Remove the needle cover.
Fig. 2-1 Removal of Parts
0.1 mL syringeSample loop

2.2.2
2 - 3
Fig. 2-2 Removal of Needle Cover
Fig. 2-3 Removal of Parts
Needle cove
r
Setscrew 1 Setscrew 2Sample loop

2.2 Setup
2 - 4
2.2.3 How to Mount
0.5 mL/1 mL/
2.5 mL Syringe
Mount the following parts (see Figures 1-2, 2-4, 2-5 and 2.6)
(1) Mount the 0.5 mL/1 mL/2.5 mL syringe.
(2) Using the spanner furnished together with the L-2200/
2200U autosampler, mount the sample loop 1 (890-3481),
coupling, sample loop 2 (890-3466, 890-3468, 890-3470),
and connecting cil (890-3467, 890-3469, 890-3471).
Fig. 2-4 Mounting of 0.5 mL/1 mL/2.5 mL Syringe
NOTICE: 1. Push sample loop 1 (890-3481) completely into
the needle coupling, and then tighten setscrew 1
(see Figure 2-5).
2. Store sample loop 2 (890-3466, 890-3468, 890-
3470) and connecting coil (890-3467, 890-3469,
890-3471) in the storage space at the side of
injection valve (see Figure 2-6).
Syringe
(080-1064, 080-1592,
080-1092)
Connecting coil
(890-3467,
890-3469,
890-3471)
Coupling
Sample loop 2
(890-3466, 890-3468,
890-3470)
Sample loop 1
(890-3481)