Agilent Technologies 1260 Infinity User manual

Agilent 1260 Infinity
HPLC-Chip Cube
Interface (G4240A )
User Manual

HPLC-Chip Cube Interface User’s Guide
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Manual Part Number
G4240-90006 Rev. B
Edition
05/2012
Printed in Germany
Agilent Technologies
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76337 Waldbronn
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HPLC-Chip Cube Interface User’s Guide 3
In This Guide…
This manual gives an introduction to HPLC-Chip technology
and explains how to install, maintain and troubleshoot you
HPLC-Chip Cube.
1Introduction to HPLC-Chip Technology
2Introduction to the Chip Cube
3Site Preparation
4Installation
5Operation
6Maintenance Procedures
7Consumables and Maintenance Parts
Appendix A Appendix A Safety Information

4 HPLC-Chip Cube Interface User’s Guide

Contents
HPLC-Chip Cube Interface User’s Guide 5
Content
1 Introduction to HPLC-Chip Technology 9
Overview 10
Chip Types 13
The MS Calibration and Diagnosis Chip, Part No. G4240-61001 13
The Protein ID Chip, Part No. G4240-62001 14
Modes of Operation 14
More Chips 18
2 Introduction to the Chip Cube 19
Intended Use 20
Introduction 21
Power Switch 22
Safety Features 23
Main Assemblies 24
Principle of Operation 26
Flow Connections 29
3 Site Preparation 31
Site Requirements for the G4240A Chip Cube 32
Power Considerations 32
Bench Space 32
Environment 33
Sound Emission 34
Specifications of the G4240A HPLC-Chip Cube 34
4 Installation 37
Unpacking the Agilent G4240A HPLC-Chip MS Interface 38
Damaged Packaging 38
Delivery Checklist 38

6 HPLC-Chip Cube Interface User’s Guide
Contents
Accessory Kit 40
System Setup Considerations 42
Single Stack Setup 43
Tw i n S t a c k s 45
Preparation of Existing Systems 47
Chip Cube Hardware Installation 49
5Operation53
Chip Loading and Unloading 54
Configuration 55
Configure Loading Pump and Analytical Pump 55
Injection Flush Volume 56
Injector Flush Volume and the use of Injector Programs 57
Operational Parameters 60
Setting up Chip Cube Parameters 61
Chip (Valve Positions / Tip) 61
Time 62
Time Table 63
Instrument Actuals 63
Chip Details Screen 64
Spray Ignition 66
Conditions to avoid 69
6 Maintenance Procedures 71
Maintenance Overview 72
Replacing Capillaries 73
Cleaning the Valve Rotors (quick procedure) 76
Updating the Chip Cube Firmware 79

Contents
HPLC-Chip Cube Interface User’s Guide 7
7 Consumables and Maintenance Parts 81
HPLC-Chips 82
Capillaries 83
Fittings 84
Infusion Spare Parts 85
Valve Rotor Cleaning Tools and Replacement Parts 86
8 Appendix A
Safety Information 87
Safety Information 88
Power Cords 88
Operation 89
Safety Symbols 90
Chemical and Biological Safety 91
Lithium Batteries Information 92
The Waste Electrical and Electronic Equipment (WEEE) Directive
(2002/96/EC) 93
Legal Notice 94

8 HPLC-Chip Cube Interface User’s Guide
Contents

9
Agilent 1260 Infinity HPLC-Chip Cube MS Interface (G4240A)
Chip Cube User’s Guide
Agilent Technologies
1
Introduction to HPLC-Chip Technology
Overview 10
Chip Types 13
The MS Calibration and Diagnosis Chip, Part No. G4240-61001 13
The Protein ID Chip, Part No. G4240-62001 14
This chapter will provide you with a basic understanding of Agilent’s
HPLC-Chip technology and an overview of the currently available chips.

10 HPLC-Chip Cube Interface User’s Guide
1 Introduction to HPLC-Chip Technology
Overview
Overview
Traditional nanospray mass spectrometry has proven it's potential as a
cost-effective, sensitive and reproducible technique for the identification of
peptides at femtomol to atomol levels. However, connecting nano capillaries,
columns and valves frequently is a tedious procedure and requires user skills
and routine. When handled incorrectly, nano flow connections are prone to
leakage which are difficult to detect and result in poor system performance
and extended downtime of the complete system. Quartz nano spray needles
are prone to blockages and require frequent replacement.
With the invention of HPLC-Chip technology, Agilent is significantly reducing
the need for user interaction and making nanospray a rugged state-of-the-art
technology.
The cornerstone of chip technology is the HLPC-Chip, a 3-dimensional
structure made of sandwiched polyimide films. Grooves of specific dimensions
are laser-ablated into a layer of polyimide film. The lamination of a top and
bottom layer then forms channels of trapezoidal or triangular shape inside the
chip which can either be used as capillaries or, if filled with packing material,
as nanocolumns. One end of the chip tapers off into a polyimide nanospray
emitter.