Agilent Technologies G1313A User manual

s1
Agilent 1100 Series
Standard, Micro and
Preparative
Autosamplers
Reference Manual

Agilent Technologies
Hewlett-Packard-Strasse 8
76337 Waldbronn
Germany
 opyright Agilent
Technologies 2001
All rights reserved.
Reproduction,
adaption, or translation
without prior written
permission is
prohibited, except as
allowed under the
copyright laws.
Part No. G1313-90004
Edition 08/01
Printed in Germany
Warranty
The information
contained in this
document is subject to
change without notice.
Agilent Technologies
makes no warranty of
any kind with regard
to this material,
including, but not
limited to, the implied
warranties or
merchantability and
fitness for a particular
purpose.
Agilent Technologies
shall not be liable for
errors contained herein
or for incidental or
consequential damages
in connection with the
furnishing,
performance, or use of
this material.
WARNI NG
For details of safety,
see Safety Information
on page 279.
Warning Symbols Used
In This Book
The apparatus is
marked with this
symbol when the user
should refer to the
instruction manual in
order to prevent risk of
harm to the operator
and to protect the
apparatus against
damage.
!

Reference Manual
Agilent 1100 Series Standard, Micro and Preparative Autosamplers

4
In This Book
This manual contains technical reference information about the Standard,
Micro and Preparative Autosamplers and their respective Thermostatted
version. The manual describes the following:
• installing the autosampler,
• optimizing performance,
• troubleshooting and test functions,
• repairing the autosampler,
• parts and materials,
• introduction to the autosampler,
• theory of operation and
• control module screens.

Contents
5
1 Installing the Autosampler
Site requirements and installation of the autosampler and
thermostatted autosampler 13
Site Requirements 14
Unpacking the Autosampler 17
Optimizing the Stack onfiguration 22
Installing the Autosampler 26
Installing the Thermostatted Autosampler 28
Flow onnections 31
Sample Trays 32
hoice of Vials and aps 35
Transporting the Autosampler 38
2 Optimizing erformance
How to optimize the autosamplers to achieve best results 3
Optimization for Lowest arry-over 41
Fast Injection ycle and Low Delay Volume 44
Precise Injection Volume 46
hoice of Rotor Seal 48
3 Troubleshooting and Test Functions
The modules built-in troubleshooting and test functions 4
Status Indicators 51
Power Supply Indicator 52
Instrument Status Indicator 52

6
Contents
Error Messages 53
Timeout 54
Shutdown 55
Remote Timeout 56
Sychronization Lost 57
Leak 58
Leak Sensor Open 59
Leak Sensor Short 60
ompensation Sensor Open 61
ompensation Sensor Short 62
Fan Failed 63
Open over 64
Restart Without over 65
Arm Movement Failed 66
Valve to Bypass Failed 67
Valve to Mainpass Failed 68
Needle Up Failed 69
Needle Down Failed 70
Missing Vial 71
Initialization Failed 72
Metering Home Failed 73
Motor Temperature 74
Initialization with Vial 75
Safety Flap Missing 76
Vial in Gripper 77
Missing Wash Vial 78
Invalid Vial Position 79
Maintenance Functions 80
hange Needle 81
hange Piston 83

Contents
7
Park Arm (Park Gripper) 84
hange Gripper ( hange Arm) 85
Tray Alignment 87
Step Commands 89
Troubleshooting Guide for the Sample Transport
Assembly 94
Intermittent lock-ups with or without vial in the gripper
fingers 95
Jittery (shaky) movement in X and or theta axes and/or when
the needle goes through the gripper arm into the via 97
Poor alignment, seen during vial pickup and vial replacement
and/or when the needle hits the gripper arm 99
4 Repairing the Autosampler
Instructions on simple, routine repair procedures as well as
more extensive repairs requiring exchange of internal
parts 101
Updating the Firmware 103
leaning the Autosampler 104
Using the ESD Strap 105
Overview of Main Repair Procedures 106
Simple Repairs 107
Needle Assembly 108
Needle-Seat Assembly 111
Stator Face 114

8
Contents
Rotor Seal 117
Metering Seal and Plunger 120
Gripper Arm 123
Interface Board 125
Exchanging Internal arts 126
Assembling the Main over 127
Top over and Foam 128
Installing the Top over and Foam 130
Transport Assembly 132
Sampling Unit 134
Injection-Valve Assembly 140
Metering-Drive Motor and Belt 142
Needle-Drive Motor and Belt 144
Fan 146
ASM Board 148
SUD Board 152
Power Supply 154
Leak Sensor 157
5 arts and Materials
Detailed illustrations and lists for identification of parts and
materials 15
Main Assemblies 160
Sampling Unit Assembly 162
Analytical-Head Assembly 165
Injection-Valve Assembly 168
Sheet Metal Kit 170
over Parts 171

Contents
9
Foam Parts 172
Power and Status Light Pipes 173
Leak System Parts 174
Vial Trays 175
Autosampler Accessory Kit G1313-68705 177
Thermostatted Autosampler Accessory Kit G1329-68705 178
Micro Thermostatted Autosampler Accessory Kit G1329-
68715 179
Preparative ALS Accessory Kit G2260-68705 180
Maintenance Kit G1313-68709 181
Multi-Draw Kit G1313-68711 182
900 µl Injection Upgrade Kit G1363A for G1313A / 29A 183
External Tray G1313-60004 184
Cable Overview 185
Analog ables 187
Remote ables 189
B D ables 195
Auxiliary able 197
AN able 197
External ontact able 198
RS-232 able Kit 199
LAN ables 200
6 Introduction to the Autosampler
An introduction to the standard, micro and preparative au-
tosamplers and thermostatted autosamplers 201
Introduction to the Autosampler 202
Sampling Sequence 204

10
Contents
Sampling Unit 207
Transport Assembly 210
Early Maintenance Feedback (EMF) 212
Electrical onnections 214
7 Theory of Operation
Theory of operation of mechanical hardware, electronics,
and instrument interfaces 217
Autosampler Control and Electronics 218
Position and Movement Sensors 219
Autosampler Main Board (ASM) 220
Firmware Description 226
Optional Interface Boards 228
Interfaces 231
Setting the 8-bit onfiguration Switch 236
The Main Power Supply Assembly 241
8 Introduction to the Control Module
Introduction to the screens available for operation of the Ag-
ilent 1100 Series autosamplers with the control module 245
Screens available from the Analysis screen 247
Screens available from the System screen 258
Screens available from the Records screen 260
Diagnostics and Tests 264

Contents
11
9 Specifications
Performance specifications of the Agilent 1100 Series Stan-
dard, Micro and Preparative Autosamplers 26
Performance Specifications 270
10 Legal, Safety and Warranty Information
Warranty Statement 276
Safety Information 27
Lithium Batteries Information 282
Radio Interference 283
Sound Emission 283
Solvent Information 284
Agilent Technologies on Internet 285

12
Contents

1
1Installing the
Autosampler
Site requirements and installation of the autosampler and
thermostatted autosampler

14
Installing the Autosampler
Site Requirements
A suitable site environment is important to ensure optimal performance of
the autosampler.
ower Consideration
The autosampler power supply has wide-ranging capability. onsequently
there is no voltage selector in the rear of the autosampler. There are also
no externally accessible fuses, because automatic electronic fuses are
implemented in the power supply.
The thermostatted autosampler comprises two modules, the autosampler
and the thermostat (G1330B). Both modules have a separate power supply
and a power plug for the line connections. The two modules are connected
by a control cable and both are turned on by the autosampler module.
WARNING To disconnect the autosampler from line power, unplug the power cord.
The power supply still uses some power, even if the power switch on the
front panel is turned off.
WARNING To disconnect the thermostatted autosampler from line power, unplug
the power cord from the autosampler and the ALS thermostat. The
power supplies still use some power, even if the power switch on the
front panel is turned off. lease make sure that it is always possible to
access the power plug.
WARNING Shock hazard or damage of your instrumentation can result if the
devices are connected to a line voltage higher than specified.

15
Installing the Autosampler
Site Requirements
ower Cords
Your autosampler is delivered with a power cord which matches the wall
socket of your particular country or region. The plug on the power cord
which connects to the rear of the instrument is identical for all types of
power cord.
WARNING Never operate your instrumentation from a power outlet that has no
ground connection. Never use a power cord other than the power cord
designed for your region.
WARNING Never use cables other than the ones supplied by Agilent Technologies
to ensure proper functionality and compliance with safety or EMC
regulations.
Bench Space
The autosampler dimensions and weight (see Table 1) allow the
instrument to be placed on almost any laboratory bench. The instrument
requires an additional 2.5 cm (1.0 inch) of space on either side, and
approximately 8 cm (3.1 inches) at the rear for the circulation of air, and
room for electrical connections. Ensure the autosampler is installed in a
horizontal position.
The thermostatted autosampler dimensions and weight (see ) allow the
instrument to be placed on almost any laboratory bench. The instrument
requires an additional 25 cm (10 inches) of space on either side for the
circulation of air, and approximately 8 cm (3.1 inches) at the rear for
electrical connections. Ensure the autosampler is installed in a level
position.
If a complete Agilent 1100 Series system is to be installed on the bench,
make sure that the bench is designed to carry the weight of all the
modules. For a complete system including the thermostatted autosampler
it is recommended to position the modules in two stacks, see “Optimizing
the Stack onfiguration” on page 22. Make sure that in this configuration
there is 25 cm (10 inches) space on either side of the thermostatted
autosampler for the circulation of air.

16
Installing the Autosampler
Site Requirements
Environment
Your autosampler will work within specifications at ambient temperatures
and relative humidity as described in Table 1.
CAUTION Do not store, ship or use your autosampler under conditions where
temperature fluctuations may cause condensation within the autosampler.
ondensation will damage the system electronics. If your autosampler was
shipped in cold weather, leave it in its box, and allow it to warm up slowly
to room temperature to avoid condensation.
WARNING Using the autosampler at environmental temperatures higher than 50 °C
(122 °F) may cause the rear panel to become hot.
Table 1 Physical Specifications - Autosampler (G1313A / G1329A / G1389A / G2260A)
7\SH 6SHFLILFDWLRQ &RPPHQWV
Weight 14.2 kg (31.3 lbs)
Dimensions
(height × width × depth)
200 × 345 × 435 mm
(8 × 13.5 × 17 inches)
Line voltage 100 – 120 or 220 – 240 VAC, ± 10 % Wide-ranging capability
Line frequency 50 or 60 Hz, ±5%
Power consumption (apparent power)
Power consumption (active power)
300 VA
200 W
Maximum
Maximum
Ambient operating temperature 4–55 °C (41 –131 °F) see WARNING on page 16
Ambient non-operating temperature -40 to 70 °C (-4 to 158 °F)
Humidity <95%,at25–40 °C (77 –104 °F) Non-condensing
Operating Altitude Up to 2000 m (6500 ft)
Non-operating altitude Up to 4600 m (14950 ft) For storing the autosampler
Safety standards: IEC, CSA, UL Installation Category II, Pollution Degree 2

17
Installing the Autosampler
Unpacking the Autosampler
Unpacking the Autosampler
CAUTION If you need to ship the autosampler at a later date, always park the
transport assembly before shipment (see “Transporting the Autosampler”
on page 38).
Damaged ackaging
Upon receipt of your autosampler, inspect the shipping containers for any
signs of damage. If the containers or cushioning material are damaged,
save them until the contents have been checked for completeness and the
autosampler has been mechanically and electrically checked. If the
shipping container or cushioning material is damaged, notify the carrier
and save the shipping material for the carriers inspection.
CAUTION If there are signs of damage to the autosampler, please do not attempt to
install the autosampler.
Delivery Checklist
Ensure all parts and materials have been delivered with the autosampler.
The instrument box contains the instrument and an Accessory kit. A
separate box contains the reference manual and the power cable.
In Table 3, Table 4, Table 5 and Table 6 are listed the content of each
accessory kit.
Please report missing or damaged parts to your local Agilent Technologies
sales and service office.

18
Installing the Autosampler
Unpacking the Autosampler
Table 2 G1313A - Autosampler Accessory Kit Contents G1313-68705
'HVFULSWLRQ 3DUW1XPEHU
Tubing assembly 5063-6527
Filter promo kit 5064-8240
CAN cable 5181-1516
Screw cap vials, clear 100/pk 5182-0714
Blue screw caps 100/pk 5182-0717
Label halftray 5989-3890
Vial instruction sheet no PN
Wrenches 1/4 - 5/16 inch 8710-0510
Rheotool socket wrench 1/4 inch 8710-2391
Hex key 4 mm, 15 cm long, T-handle 8710-2392
Hex key 9/64 mm, 15 cm long, T- handle 8710-2394
Hex key 2.5 mm, 15 cm long, straight handle 8710-2412
ESD wrist strap 9300-1408
Finger caps (x3)*
* Reorder gives pack of 15
5063-6506
Tray for 40 x 2 ml vials G1313-44502
Tray for 15 x 6 ml vials G1313-44503
Capillary 0.17 mm 180 mm G1313-87305

19
Installing the Autosampler
Unpacking the Autosampler
Table 3 G1329A - Thermostatted Autosampler Accessory Kit Contents G1329-68705
'HVFULSWLRQ 3DUW1XPEHU
Tubing assembly 5063-6527
Filter promo kit 5064-8240
CAN cable, 1 m long 5181-1519
Screw cap vials, clear 100/pk 5182-0714
Blue screw caps 100/pk 5182-0717
Label halftray 5989-3890
Vial instruction sheet no PN
Wrenches 1/4 - 5/16 inch 8710-0510
Rheotool socket wrench 1/4 inch 8710-2391
Hex key 4 mm, 15 cm long, T-handle 8710-2392
Hex key 9/64 mm, 15 cm long, T- handle 8710-2394
Hex key 2.5 mm, 15 cm long, straight handle 8710-2412
ESD wrist strap 9300-1408
Finger caps (x3)*
* Reorder gives pack of 15
5063-6506
Front door cooled autosampler G1329-40301
Air channel adapter G1329-43200
Cover insulation no PN
Capillary 0.17 mm, 900 mm G1329-87300
Capillary heat exchanger 01090-87306
Note for 1100 Series Autosampler door upgrade no PN

20
Installing the Autosampler
Unpacking the Autosampler
Table 4 G1389A - Micro Thermostatted Autosampler Accessory Kit Contents G1329-68715
'HVFULSWLRQ 3DUW1XPEHU
Tubing assembly 5063-6527
CAN cable, 1 m long 5181-1519
Screw cap vials, clear 100/pk 5182-0714
Blue screw caps 100/pk 5182-0717
Label halftray 5989-3890
Vial instruction sheet no PN
Fitting 79814-22406
Hex Key 8710-0060
Wrench 4 mm both ends 8710-1534
Wrenches 1/4 - 5/16 inch 8710-0510
Rheotool socket wrench 1/4 inch 8710-2391
Hex key 4 mm, 15 cm long, T-handle 8710-2392
Hex key 9/64 mm, 15 cm long, T- handle 8710-2394
Hex key 2.5 mm, 15 cm long, straight handle 8710-2412
ESD wrist strap 9300-1408
Finger caps (x3)*
* Reorder gives pack of 15
5063-6506
Torque adapter G1315-45003
Front door cooled autosampler G1329-40301
Air channel adapter G1329-43200
Cover insulation no PN
Extended loop capillary 0.25 mm, 180 mm G1329-87302
Fused silica capillary 0.050 mm, 500 mm G1375-87304