ibidi 12150 User manual

Instruction Manual
ibidi Heating System Multiwell Plate –
Silver Line
Version 1.0
.com
12150 ibidi Heating System Multiwell Plate – Silver Line
1
3
4
6
25
ibidiTemperature Controller
Plate
I 38.0
S[ 38.0] Run
Lid Glass
42.1
42.0 36.9
37.0
Setup
N.C.

ibidi Heating System Multiwell Plate – Silver Line
Version 1.0 (August 1, 2022) 2 Instruction Manual

ibidi Heating System Multiwell Plate – Silver Line
Contact
ibidi GmbH
Lochhamer Schlag 11
82166 Gr¨ afelfing
Germany
Phone: +49 89 / 520 46 17 - 0
Fax: +49 89 / 520 46 17 - 59
E-mail: [email protected]
Internet: ibidi.com
Instruction Manual 3 Version 1.0 (August 1, 2022)

ibidi Heating System Multiwell Plate – Silver Line
Contents
1 Preamble 6
1.1 Introduction ........................................... 6
1.2 Safety Symbols ......................................... 6
1.3 Nomenclature .......................................... 7
1.4 Specifications .......................................... 7
1.5 Disclaimer ............................................ 9
1.6 Safety Considerations ...................................... 9
1.7 Limited Warranty ........................................ 11
1.8 Transporting the ibidi Heating System Multiwell Plate .................. 11
1.9 Repairing the ibidi Heating System Multiwell Plate .................... 12
1.10 Waste Disposal – WEEE/RoHS Compliance Statement .................. 12
1.10.1 EU Directive WEEE ................................... 12
1.10.2 EU Directive RoHS ................................... 12
1.11 Regulatory Statement ...................................... 13
2 Intended Use 14
3 Principle 14
4 Equipment 16
4.1 Components of the ibidi Heating System Multiwell Plate ................. 16
4.2 Stage Top Incubator Overview ................................ 17
4.3 ibidi Temperature Controller – Silver Line .......................... 18
4.4 Heated Plate ........................................... 20
4.5 Heated Lid ............................................ 21
4.6 Cable Clip for Optional Use .................................. 21
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ibidi Heating System Multiwell Plate – Silver Line
5 Operation 23
5.1 Installation and Connection of the Components ...................... 23
5.2 Start Operation of the ibidi Heating System Multiwell Plate ................ 24
5.3 Setting Parameters in the Front Display ........................... 24
5.3.1 Run/Stop Mode .................................... 25
5.3.2 Incubation Parameters ................................. 25
5.3.3 Select Temperature Sensor ............................... 26
5.3.4 Setup Menu ....................................... 26
5.4 Temperature Adjustment in the Sample ........................... 27
5.5 Sample Preparation ....................................... 29
6 IncuControl Software 30
7 Maintenance 31
7.1 Disinfection and Cleaning ................................... 31
7.2 Influence of Ambient Temperature and Ventilation ..................... 31
8 Troubleshooting 32
8.1 Focus not Stable ......................................... 32
8.2 Evaporation too High ...................................... 32
8.3 Condensation Inside the Stage Top Incubator ........................ 32
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ibidi Heating System Multiwell Plate – Silver Line
1 Preamble
1.1 Introduction
This manual is your guide to using the ibidi Heating System Multiwell Plate – Silver Line for cell cul-
ture experiments on an optical microscope. It instructs first-time users on how to use the instrument,
and serves as a reference for experienced users.
Before using the ibidi Heating System Multiwell Plate – Silver Line, please read this instruction man-
ual carefully and make sure that the contents are fully understood. This manual should be easily
accessible to the operator at all times during instrument operation. If this manual gets lost, see the
online version on ibidi.com or request a replacement via [email protected].
To ensure safe operation, the ibidi Heating System Multiwell Plate – Silver Line must only be oper-
ated with the supplied components and according to the instruction manual.
For Research Use Only! Not for use in diagnostic procedures.
1.2 Safety Symbols
Note that the signal words WARNING,CAUTION and NOTE have specific meanings in this man-
ual. Do not proceed beyond a signal word until you have performed the indicated actions. Warning
messages in the text are displayed in a gray shaded box. Please see section 1.6 for general safety
considerations.
WARNING – A potentially hazardous situation which, if not avoided, could result
in serious injury or even death.
CAUTION – A potentially hazardous situation which, if not avoided, could result in
minor or moderate injury. It is also used to alert against damaging the equipment or
the instrument.
NOTE – Additional information to help achieve optimal instrument and assay per-
formance.
Symbols on the product identification label and back panel of the device:
CE Marking: This symbol indicates the product’s compliance with EU legislation.
This label is positioned on the back of the device and prompts you to read the manual
before using the device.
Product disposal: The symbol indicates that this product must be recycled/disposed
of separately from other household waste. See page 12 for details.
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ibidi Heating System Multiwell Plate – Silver Line
1.3 Nomenclature
1
3
4
6
25
ibidiTemperature Controller
1
Plate
I 38.0
S[ 38.0] Run
Lid
42.1
42.0 Setup
N.C.
ibidi Temperature Con-
troller – Silver Line Incubation Chamber Heated Plate Heated Lid
Where adequate, the ibidi Heating System Multiwell Plate – Silver Line is hereafter abbreviated and
referred to as ibidi Heating System Multiwell Plate in body text and figure captions. The ibidi Tem-
perature Controller – Silver Line is hereafter abbreviated and referred to as Temperature Controller,
where adequate.
1.4 Specifications
Only operate the Heating System in the range of the specifications given below:
Table 1: Specifications of the ibidi Heating System Multiwell Plate
Electrical Specifications Power Supply
Protection class I
International protection marking
(IEC 60529)
IP 20
Overvoltage category II
Pollution degree 2
Input voltage and current AC 100–240 V, 50/60 Hz, 2 A, 36–40 W
Output voltage and current DC 24 V, 6.67 A, 160 W
Electrical Specifications ibidi Temperature Controller – Silver Line
Protection class III
Ingress protection rating IP 20
Overvoltage category I
Pollution degree 2
Input voltage and current DC 24 V, 6.67 A, 160 W
Output voltage and current per
heating channel
DC max. 24 V, max. 2 A
Operating and Storage Conditions
Operating site Indoor use only
Operating temperature 18–30°C/64–86°F (at least 5°C/9°F less than set tempera-
ture)
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ibidi Heating System Multiwell Plate – Silver Line
Table 1: (continued)
Operating humidity max. 80% relative humidity (RH)
Operating altitude max. 2000 m (atmospheric pressure 800–1060 hPa/11.6–15.4
psi)
Storage conditions -5–50°C/23–122°F, humidity <60% relative humidity (RH)
Outer Dimensions and Characteristics of the Components
ibidi Temperaure Controller – 90 mm × 170 mm × 230 mm
Silver Line Weight: 1.75 kg/3.86 lbs
Heated Lidof Incubation Chamber
Multiwell Plate
25 mm × 160 mm × 108 mm
Length of cable: 1.8 m total, 1.5 m to converter box
Connector to Gas Incubation: Female Luer Lock
Heated Plateof Incubation Cham-
ber Multiwell Plate
9 mm × 160 mm × 130 mm
K-Frame fitting: 160 mm × 110 mm
Observation area: 107.5 mm × 72.5 mm
Length of cable: 1.8 m total, 1.5 m to converter box
Heated Plate assembled with Height: 30 mm
Heated Lid Weight: 430 g/0.95 lbs
USB cable Length: 1.8 m
Power supply cable Length: 2.0 m (power supply to wall)
Length: 1.2 m (power supply to device)
Temperature Control Range
Heated Lid 5 degree above ambient temperature (min. 18°C) to 45°C
Heated Plate 5 degree above ambient temperature (min. 18°C) to 45°C
Heated Glass Bottom 5 degree above ambient temperature (min. 18°C) to 45°C
Temperature deviation max. 2°C between wells of a multiwell plate
Sample Temperature
Temperature stability ±0.2°C
Recommended temperatures be-
fore adjustment
See Section 5.4
USB Interface
Connector type at controller USB 2.0 Connector Type B
Recommended USB cable Tripp Lite UR022-006 (shielded)
USB driver for PC FTDI VCP driver
Optical Properties Heated Lid and Heated Glass Bottom
Glass Selected HQ Floatglass
Thickness of the glass plate 1.1 mm/0.7 mm
Refractive index glass nD= 1.520 (588 nm)
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ibidi Heating System Multiwell Plate – Silver Line
Table 1: (continued)
Thickness of ITO coating 100 nm
Passivation layer Thickness: 20–25 nm
Refractive index ITO with passiva-
tion layer
1.95
Requirements for Objective Lenses
Minimum 1 mm working distance for coverslip bottom plates (e.g., ibidi µ-Plate 96 Well)
Minimum 2 mm working distance for 0.7 mm thick bottom plates
The sample focal plane in the Incubation Chamber Multiwell Plate lies up to 3.5 mm above
the usual focal plane of the sample. Therefore, the objective must have at least 3.5 mm of
travel distance along the focal axis for imaging.
Microscope Requirements
Working distance condenser ≥26 mm
Stage opening 160 mm × 110 mm
1.5 Disclaimer
• ibidi shall not be held liable, either directly or indirectly, for any damage incurred as a result of
product use.
• The contents of this manual are subject to change without notice for product improvement.
• This manual is considered complete and accurate at publication.
• This manual does not guarantee the validity of any patent rights or other rights.
• If an ibidi software program doesn’t function properly, this may be caused by a conflict from
another program operating on the computer. In this case, take corrective action by uninstalling
the conflicting product(s).
• ibidi is a registered trademark of ibidi GmbH in Germany and other countries.
1.6 Safety Considerations
WARNING
• Only operate the ibidi Heating System Multiwell Plate with the supplied components.
• Only use the cables and plugs delivered with the system. The power plug of the control unit
must be inserted in an outlet with a ground (earth) contact.
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ibidi Heating System Multiwell Plate – Silver Line
• Do not replace detachable power cables by power cables with inadequate specifications. By
violating these instructions, you risk electric shock and fire.
• Only use extension cables that have a protective ground wire.
• Do not operate the ibidi Heating System Multiwell Plate under conditions that pose a risk of
explosion, implosion, or the release of gases.
• Do not place flammable solids, liquids, gases, or gas outlets near the system (e.g., matches,
ethanol, propane gas, solvents). Do not bring these products in contact with any other compo-
nent of the system either.
• Do not operate a damaged ibidi Heating System Multiwell Plate. If the housing seems damaged
or something is rattling inside the controller, contact the ibidi technical support for repair.
• Only operate the Heated Lid and Heated Plate properly installed on a microscope stage.
• Some accessible parts of the Heated Plate and Heated Lid can reach temperatures up to 55°C.
Avoid touching the temperature-controlled parts of the system when you have set the ibidi
Temperature Controller – Silver Line to high temperatures.
CAUTION
• Ensure that the external power supply is easily accessible. The ibidi Heating System Multiwell
Plate must be installed in a manner such that none of its components hinders access to the
external power supply.
• Immediately replace damaged cords, plugs, or cables to avoid risk of personal injury or damage
to the instrument.
• Only ibidi technical staff and technical staff instructed by ibidi are permitted to open and ser-
vice the ibidi Heating System Multiwell Plate.
• The external power supply should not be brought into contact with moisture. If the housing is
damaged, the external power supply should not be used.
• Avoid strong magnetic fields and sources of high frequency. The ibidi Heating System Mul-
tiwell Plate might not function properly when located near a strong magnetic field or high
frequency source.
• Avoid vibrations from vacuum pumps, centrifuges, electric motors, processing equipment, and
machine tools.
• Avoid dust and corrosive gas. Do not install the ibidi Heating System Multiwell Plate where it
could be exposed to high levels of dust or to outside air or ventilation outlets.
• Install the ibidi Heating System Multiwell Plate in a location that enables easy access for main-
tenance.
• Do not place heavy objects on the instrument.
• Install the ibidi Temperature Controller – Silver Line in a horizontal and stable position, which
includes a table, bench or desk upon which the instrument is installed.
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