ibidi 12150 User manual

Instruction Manual
ibidi Heating System Multiwell Plate –
Silver Line
Version 1.0
.com
12150 ibidi Heating System Multiwell Plate – Silver Line
1
3
4
6
25
ibidiTemperature Controller
Plate
I 38.0
S[ 38.0] Run
Lid Glass
42.1
42.0 36.9
37.0
Setup
N.C.

ibidi Heating System Multiwell Plate – Silver Line
Version 1.0 (August 1, 2022) 2 Instruction Manual

ibidi Heating System Multiwell Plate – Silver Line
Contact
ibidi GmbH
Lochhamer Schlag 11
82166 Gr¨ afelfing
Germany
Phone: +49 89 / 520 46 17 - 0
Fax: +49 89 / 520 46 17 - 59
E-mail: [email protected]
Internet: ibidi.com
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ibidi Heating System Multiwell Plate – Silver Line
Contents
1 Preamble 6
1.1 Introduction ........................................... 6
1.2 Safety Symbols ......................................... 6
1.3 Nomenclature .......................................... 7
1.4 Specifications .......................................... 7
1.5 Disclaimer ............................................ 9
1.6 Safety Considerations ...................................... 9
1.7 Limited Warranty ........................................ 11
1.8 Transporting the ibidi Heating System Multiwell Plate .................. 11
1.9 Repairing the ibidi Heating System Multiwell Plate .................... 12
1.10 Waste Disposal – WEEE/RoHS Compliance Statement .................. 12
1.10.1 EU Directive WEEE ................................... 12
1.10.2 EU Directive RoHS ................................... 12
1.11 Regulatory Statement ...................................... 13
2 Intended Use 14
3 Principle 14
4 Equipment 16
4.1 Components of the ibidi Heating System Multiwell Plate ................. 16
4.2 Stage Top Incubator Overview ................................ 17
4.3 ibidi Temperature Controller – Silver Line .......................... 18
4.4 Heated Plate ........................................... 20
4.5 Heated Lid ............................................ 21
4.6 Cable Clip for Optional Use .................................. 21
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ibidi Heating System Multiwell Plate – Silver Line
5 Operation 23
5.1 Installation and Connection of the Components ...................... 23
5.2 Start Operation of the ibidi Heating System Multiwell Plate ................ 24
5.3 Setting Parameters in the Front Display ........................... 24
5.3.1 Run/Stop Mode .................................... 25
5.3.2 Incubation Parameters ................................. 25
5.3.3 Select Temperature Sensor ............................... 26
5.3.4 Setup Menu ....................................... 26
5.4 Temperature Adjustment in the Sample ........................... 27
5.5 Sample Preparation ....................................... 29
6 IncuControl Software 30
7 Maintenance 31
7.1 Disinfection and Cleaning ................................... 31
7.2 Influence of Ambient Temperature and Ventilation ..................... 31
8 Troubleshooting 32
8.1 Focus not Stable ......................................... 32
8.2 Evaporation too High ...................................... 32
8.3 Condensation Inside the Stage Top Incubator ........................ 32
Instruction Manual 5 Version 1.0 (August 1, 2022)

ibidi Heating System Multiwell Plate – Silver Line
1 Preamble
1.1 Introduction
This manual is your guide to using the ibidi Heating System Multiwell Plate – Silver Line for cell cul-
ture experiments on an optical microscope. It instructs first-time users on how to use the instrument,
and serves as a reference for experienced users.
Before using the ibidi Heating System Multiwell Plate – Silver Line, please read this instruction man-
ual carefully and make sure that the contents are fully understood. This manual should be easily
accessible to the operator at all times during instrument operation. If this manual gets lost, see the
online version on ibidi.com or request a replacement via [email protected].
To ensure safe operation, the ibidi Heating System Multiwell Plate – Silver Line must only be oper-
ated with the supplied components and according to the instruction manual.
For Research Use Only! Not for use in diagnostic procedures.
1.2 Safety Symbols
Note that the signal words WARNING,CAUTION and NOTE have specific meanings in this man-
ual. Do not proceed beyond a signal word until you have performed the indicated actions. Warning
messages in the text are displayed in a gray shaded box. Please see section 1.6 for general safety
considerations.
WARNING – A potentially hazardous situation which, if not avoided, could result
in serious injury or even death.
CAUTION – A potentially hazardous situation which, if not avoided, could result in
minor or moderate injury. It is also used to alert against damaging the equipment or
the instrument.
NOTE – Additional information to help achieve optimal instrument and assay per-
formance.
Symbols on the product identification label and back panel of the device:
CE Marking: This symbol indicates the product’s compliance with EU legislation.
This label is positioned on the back of the device and prompts you to read the manual
before using the device.
Product disposal: The symbol indicates that this product must be recycled/disposed
of separately from other household waste. See page 12 for details.
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ibidi Heating System Multiwell Plate – Silver Line
1.3 Nomenclature
1
3
4
6
25
ibidiTemperature Controller
1
Plate
I 38.0
S[ 38.0] Run
Lid
42.1
42.0 Setup
N.C.
ibidi Temperature Con-
troller – Silver Line Incubation Chamber Heated Plate Heated Lid
Where adequate, the ibidi Heating System Multiwell Plate – Silver Line is hereafter abbreviated and
referred to as ibidi Heating System Multiwell Plate in body text and figure captions. The ibidi Tem-
perature Controller – Silver Line is hereafter abbreviated and referred to as Temperature Controller,
where adequate.
1.4 Specifications
Only operate the Heating System in the range of the specifications given below:
Table 1: Specifications of the ibidi Heating System Multiwell Plate
Electrical Specifications Power Supply
Protection class I
International protection marking
(IEC 60529)
IP 20
Overvoltage category II
Pollution degree 2
Input voltage and current AC 100–240 V, 50/60 Hz, 2 A, 36–40 W
Output voltage and current DC 24 V, 6.67 A, 160 W
Electrical Specifications ibidi Temperature Controller – Silver Line
Protection class III
Ingress protection rating IP 20
Overvoltage category I
Pollution degree 2
Input voltage and current DC 24 V, 6.67 A, 160 W
Output voltage and current per
heating channel
DC max. 24 V, max. 2 A
Operating and Storage Conditions
Operating site Indoor use only
Operating temperature 18–30°C/64–86°F (at least 5°C/9°F less than set tempera-
ture)
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ibidi Heating System Multiwell Plate – Silver Line
Table 1: (continued)
Operating humidity max. 80% relative humidity (RH)
Operating altitude max. 2000 m (atmospheric pressure 800–1060 hPa/11.6–15.4
psi)
Storage conditions -5–50°C/23–122°F, humidity <60% relative humidity (RH)
Outer Dimensions and Characteristics of the Components
ibidi Temperaure Controller – 90 mm × 170 mm × 230 mm
Silver Line Weight: 1.75 kg/3.86 lbs
Heated Lidof Incubation Chamber
Multiwell Plate
25 mm × 160 mm × 108 mm
Length of cable: 1.8 m total, 1.5 m to converter box
Connector to Gas Incubation: Female Luer Lock
Heated Plateof Incubation Cham-
ber Multiwell Plate
9 mm × 160 mm × 130 mm
K-Frame fitting: 160 mm × 110 mm
Observation area: 107.5 mm × 72.5 mm
Length of cable: 1.8 m total, 1.5 m to converter box
Heated Plate assembled with Height: 30 mm
Heated Lid Weight: 430 g/0.95 lbs
USB cable Length: 1.8 m
Power supply cable Length: 2.0 m (power supply to wall)
Length: 1.2 m (power supply to device)
Temperature Control Range
Heated Lid 5 degree above ambient temperature (min. 18°C) to 45°C
Heated Plate 5 degree above ambient temperature (min. 18°C) to 45°C
Heated Glass Bottom 5 degree above ambient temperature (min. 18°C) to 45°C
Temperature deviation max. 2°C between wells of a multiwell plate
Sample Temperature
Temperature stability ±0.2°C
Recommended temperatures be-
fore adjustment
See Section 5.4
USB Interface
Connector type at controller USB 2.0 Connector Type B
Recommended USB cable Tripp Lite UR022-006 (shielded)
USB driver for PC FTDI VCP driver
Optical Properties Heated Lid and Heated Glass Bottom
Glass Selected HQ Floatglass
Thickness of the glass plate 1.1 mm/0.7 mm
Refractive index glass nD= 1.520 (588 nm)
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ibidi Heating System Multiwell Plate – Silver Line
Table 1: (continued)
Thickness of ITO coating 100 nm
Passivation layer Thickness: 20–25 nm
Refractive index ITO with passiva-
tion layer
1.95
Requirements for Objective Lenses
Minimum 1 mm working distance for coverslip bottom plates (e.g., ibidi µ-Plate 96 Well)
Minimum 2 mm working distance for 0.7 mm thick bottom plates
The sample focal plane in the Incubation Chamber Multiwell Plate lies up to 3.5 mm above
the usual focal plane of the sample. Therefore, the objective must have at least 3.5 mm of
travel distance along the focal axis for imaging.
Microscope Requirements
Working distance condenser ≥26 mm
Stage opening 160 mm × 110 mm
1.5 Disclaimer
• ibidi shall not be held liable, either directly or indirectly, for any damage incurred as a result of
product use.
• The contents of this manual are subject to change without notice for product improvement.
• This manual is considered complete and accurate at publication.
• This manual does not guarantee the validity of any patent rights or other rights.
• If an ibidi software program doesn’t function properly, this may be caused by a conflict from
another program operating on the computer. In this case, take corrective action by uninstalling
the conflicting product(s).
• ibidi is a registered trademark of ibidi GmbH in Germany and other countries.
1.6 Safety Considerations
WARNING
• Only operate the ibidi Heating System Multiwell Plate with the supplied components.
• Only use the cables and plugs delivered with the system. The power plug of the control unit
must be inserted in an outlet with a ground (earth) contact.
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ibidi Heating System Multiwell Plate – Silver Line
• Do not replace detachable power cables by power cables with inadequate specifications. By
violating these instructions, you risk electric shock and fire.
• Only use extension cables that have a protective ground wire.
• Do not operate the ibidi Heating System Multiwell Plate under conditions that pose a risk of
explosion, implosion, or the release of gases.
• Do not place flammable solids, liquids, gases, or gas outlets near the system (e.g., matches,
ethanol, propane gas, solvents). Do not bring these products in contact with any other compo-
nent of the system either.
• Do not operate a damaged ibidi Heating System Multiwell Plate. If the housing seems damaged
or something is rattling inside the controller, contact the ibidi technical support for repair.
• Only operate the Heated Lid and Heated Plate properly installed on a microscope stage.
• Some accessible parts of the Heated Plate and Heated Lid can reach temperatures up to 55°C.
Avoid touching the temperature-controlled parts of the system when you have set the ibidi
Temperature Controller – Silver Line to high temperatures.
CAUTION
• Ensure that the external power supply is easily accessible. The ibidi Heating System Multiwell
Plate must be installed in a manner such that none of its components hinders access to the
external power supply.
• Immediately replace damaged cords, plugs, or cables to avoid risk of personal injury or damage
to the instrument.
• Only ibidi technical staff and technical staff instructed by ibidi are permitted to open and ser-
vice the ibidi Heating System Multiwell Plate.
• The external power supply should not be brought into contact with moisture. If the housing is
damaged, the external power supply should not be used.
• Avoid strong magnetic fields and sources of high frequency. The ibidi Heating System Mul-
tiwell Plate might not function properly when located near a strong magnetic field or high
frequency source.
• Avoid vibrations from vacuum pumps, centrifuges, electric motors, processing equipment, and
machine tools.
• Avoid dust and corrosive gas. Do not install the ibidi Heating System Multiwell Plate where it
could be exposed to high levels of dust or to outside air or ventilation outlets.
• Install the ibidi Heating System Multiwell Plate in a location that enables easy access for main-
tenance.
• Do not place heavy objects on the instrument.
• Install the ibidi Temperature Controller – Silver Line in a horizontal and stable position, which
includes a table, bench or desk upon which the instrument is installed.
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ibidi Heating System Multiwell Plate – Silver Line
• The heated glass plates of the incubation chamber can break on mechanical impact. If so, the
glass shards can lead to injuries if handled.
• Be aware that when switched on, a 10 V DC voltage is applied to the underside of the glass
on the Heated Lid. Do not touch the underside or put it in contact with anything conductive.
This could cause a short circuit that may damage the ibidi Temperature Controller – Silver Line
and/or the Heated Devices (e.g., Heated Plate, Heated Lid of Incubation Chambers).
1.7 Limited Warranty
Products manufactured by ibidi, unless otherwise specified, are warrantied for a period of one year
from the date of shipment to be free of defects in materials and workmanship. If any defects in the
product are found during this warranty period, ibidi will repair or replace the defective part(s) or
product free of charge.
This warranty does not apply to defects resulting from the following:
1. Improper or inadequate installation.
2. Improper or inadequate operation, maintenance, adjustment, or calibration.
3. Unauthorized modification or misuse.
4. Use of unauthorized tubing or fluidic connectors.
5. Use of consumables, disposables, and parts not supplied by an authorized ibidi distributor.
6. Corrosion due to the use of improper solvents, samples, or due to surrounding gases.
7. Accidents beyond ibidi’s control, including natural disasters.
This warranty does not cover consumables, such as cell culture chambers and dishes, tubes, fluidic
connectors, reagents etc.
The warranty for all parts supplied and repairs provided under this warranty expires on the warranty
expiration date of the original product.
1.8 Transporting the ibidi Heating System Multiwell Plate
The weight of the ibidi Temperature Controller – Silver Line is approx. 1.75 kg/3.86 lbs. Moving
the ibidi Temperature Controller – Silver Line during operation can pose a risk of personal injury or
damage to the instrument.
For transport, switch off the ibidi Temperature Controller – Silver Line and then disconnect the heated
components from the controller. Carry the devices carefully and avoid mechanical shocks.
WARNING – Hot surface (max. 55°C)! Do not touch Heated Lid and Heated Plate
when hot. Always disconnect the instrument from the power supply before transport
and leave the instrument to cool down for approx. 5 minutes.
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ibidi Heating System Multiwell Plate – Silver Line
1.9 Repairing the ibidi Heating System Multiwell Plate
For inquiries concerning repair service contact ibidi GmbH technical support ([email protected])
and provide the model name and serial number of your system.
CAUTION – Do not try to repair the ibidi Heating System Multiwell Plate by yourself.
Disassembly of the ibidi Heating System Multiwell Plate is not allowed. Disassembly poses a risk
of personal injury or damage to the devices. Contact ibidi technical support if there is a need to
disassemble a device.
1.10 Waste Disposal – WEEE/RoHS Compliance Statement
The European Union (EU) has enacted two directives, the first on product recycling (Waste Electrical
and Electronic Equipment, WEEE) and the second on limiting the use of certain substances (Restric-
tion on the use of Hazardous Substances, RoHS).
1.10.1 EU Directive WEEE
The ibidi Heating System Multiwell Plate must be disposed of in compliance with the WEEE Direc-
tive 2012/19/EC.
This symbol on the product is in accordance with the European Union’s Waste Electrical and Elec-
tronic Equipment (WEEE) Directive. The symbol indicates that this product must be recycled/dis-
posed of separately from other household waste. It is the end user’s responsibility to dispose of this
product by taking it to a designated WEEE collection facility for the proper collection and recycling of
the waste equipment. The separate collection and recycling of waste equipment will help to conserve
natural resources and protect human health and the environment. For more information about recy-
cling, please contact your local environmental office, an electrical/electronic waste disposal company
or distributor where you purchased the product.
1.10.2 EU Directive RoHS
RoHS conformity is declared in the EU-conformity in Section 1.11.
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ibidi Heating System Multiwell Plate – Silver Line
1.11 Regulatory Statement
Diese Erklärung bescheinigt die Übereinstimmung mit den genannten Richtlinien,
ist jedoch keine Zusicherung von Eigenschaften. Die Sicherheitshinweise der
Produktdokumentation sind zu beachten.
This declaration certifies the conformity to he specified directives but not includes
any warranted quality of the instrument. The safety documentation of the product shall
be considered in detail
EG-Konformitätserklärung
EC Declaration of Conformity
Wir / We
ibidi GmbH
Lochhamer Schlag 11
D-82166 Gräfelfing
erklären hiermit die Übereinstimmung des genannten Produktes mit der Richtlinie 2014/35/EU - Niederspannungsrichtlinie und
mit der Richtlinie 2014/30/EU über die Elektromagnetische Verträglichkeit.
Bei Änderungen am Produkt, die nicht von uns autorisiert wurden, verliert diese Erklärung ihre Gültigkeit.
We declare the compliance of the product with the requirements of the Directive 2014/35/EU - Low Voltage Directive
and with the Directive 2014/30/EU about the Electromagnetic Compatibility.
Any modification to the product, not authorized by us, will invalidate this declaration.
Laborgerät / laboratory equipment:
ibidi Temperature Controller ibiTC3-XXX with ibidi accessories
Der oben beschriebene Gegenstand erfüllt die Vorschriften der Richtlinie 2011/65/EU vom 08. Juni 2011
zur Beschränkung der Verwendung bestimmter gefährlicher Stoffe in Elektro- und Elektronikgeräten.
The object of the declaration described above is in conformity with Directive 2011/65/EU of 8 June 2011
on the restriction of the use of certain hazardous substances in electrical and electronic equipment.
Das Produkt entspricht den unten aufgeführten Normen:
The product meets the requirements of the following standards:
Sicherheitsbestimmungen für elektrische Mess-, Steuer-, Regel- und Laborgeräte - Teil 1: Allgemeine Anforderungen Safety
requirements for electrical equipment for measurement, control and laboratory use - Part 1: General requirements
Elektrische Mess-, Steuer-, Regel und Laborgeräte. EMV-Anforderungen. Allgemeine Anforderungen
Electrical equipment for measurement, control and laboratory use. EMC requirements. General requirements
Das Produkt ist gekennzeichnet mit/ The product is marked with
Dr. Valentin Kahl
Geschäftsführer
Name, Funktion Unterschrift
Gräfelfing, den 29.07.2021
Gräfelfing, 2021-07-29
Ort/Datum
Place/date
Name, Function Signature
DIN EN 610
10-1:2020
DIN EN 61326-1:2013
Instruction Manual 13 Version 1.0 (August 1, 2022)

ibidi Heating System Multiwell Plate – Silver Line
2 Intended Use
The ibidi Heating System Multiwell Plate – Silver Line is a stage top incubator for live cell imaging
that fits onto inverted microscope stages with a mounting frame K opening (110 × 160 mm). The ibidi
Heating System Multiwell Plate, consisting of an Incubation Chamber with Heated Plate and Heated
Lid and a Temperature Controller, provides a platform for keeping multiwell plates for microscopy
at a constant temperature during an experiment on a microscope.
For full control of the incubation environment, the ibidi Heating System Multiwell Plate can be com-
bined with the ibidi Gas Incubation System to regulate the CO2and O2concentrations as well as the
humidity.
The ibidi Heating System Multiwell Plate is for research use only! It is not intended for use in diag-
nostic procedures.
3 Principle
Physiological Conditions in Live Cell Imaging
Cells react sensitively to changes in their environment. Factors such as temperature, humidity, and
CO2/O2levels significantly influence the outcome of cell culture assays. In order to achieve biologi-
cally relevant and reproducible results, it is crucial to maintain optimal conditions on the microscope
stage during live cell imaging experiments.
To enable high-quality live cell imaging under physiological conditions, the following parameters
need to be considered:
Environment:
• Temperature
• Carbon Dioxide (CO2) Levels and pH of the
Medium
• Humidity and Evaporation
• Oxygen (O2) Levels
Optical Aspects:
• Condensation
• Imaging Method and Photon Dose Figure 1: Live cell imaging conditions.
The Heating System combined with the Gas Incubation System supplies complete incubator condi-
tions for your live cell imaging directly on the microscope stage.
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ibidi Heating System Multiwell Plate – Silver Line
ibidi
Gas Mixer
Gas Mixer Humidification Stage Top Incubator
Humidity
sensor
Valve
regulation
Gas mixture
(humidified)
Dry gas
Gas mixture
(dry)
Super-humidified gas
“100%+” RH
Feedback control
Air CO2N2
Dry gas mix
Humidified gas mix
Humidified and warm
gas mixture
ibidi
Temperature
Controller
Figure 2: Schematic view of the ibidi Stage Top Incubation System, combining the Heating System
with the Gas Incubation System. RH: relative humidity.
The Gas Incubation System provides a controlled atmosphere of gas (CO2or CO2/O2) as well as
a defined humidity in the emitted gas stream that is flushed continuously through the incubation
chamber. Oxygen can be reduced during the experiment, enabling the user to perform hypoxia
experiments (CO2/O2version only).
The Heated Lid and Heated Plate with the Heated Glass Bottom are designed to keep cells on–stage
in a multiwell plate at 37°C. The Heated Plate is mounted on the microscope stage. The multiwell
plate is placed directly on the Heated Glass Bottom and the Heated Lid is put on top.
The gas mixture is streamed into the chamber through the gas inlet and then slowly escapes through
the gaps between the Heated Lid, Heated Plate, and Heated Glass Bottom (small gray arrows in
Figure 3).
Cell culture vessel
with medium and cells
ibidi Coverslip Bottom
Heated Glass Bottom
Heated Plate
Heated Lid
Humidity
sensor
Gas
inlet
10x
Figure 3: Schematic cross-sectional view of the ibidi Heating System Multiwell Plate.
The Heated Lid prevents condensation effects inside the entire incubation system (Figure 4).
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ibidi Heating System Multiwell Plate – Silver Line
No condensation
Condensation
Heated Glass Bottom
Heated Plate
34°C
Heated Glass Bottom
Heated Plate
40°C
Heated Lid
Unheated Lid
37°C
37°C
Figure 4: The Heated Lid prevents condensation effects on the lid of the cell culture vessel.
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ibidi Heating System Multiwell Plate – Silver Line
4 Equipment
This section provides a brief overview over all parts of the ibidi Heating System Multiwell Plate
including a description of the characteristics.
4.1 Components of the ibidi Heating System Multiwell Plate
The components of the ibidi Heating System Multiwell Plate are listed below.
Description Drawing
ibidi Temperature Controller – Silver Line
1
3
4
6
25
ibidiTemperature Controller
1
Plate
I 38.0
S[ 38.0] Run
Lid
42.1
42.0 Setup
E N.C.
Heated Lid with electrical cable to the converter box
Heated Plate with Heated Glass Bottom and electrical cable
to the converter box
Converter box connected to the cables of the Heated Plate
and Heated Lid, with multipole connectors to the ibidi
Temperature Controller – Silver Line
Temperature Adjustment Set:
1 temperature sensor
1 perforated ibidi µ-Plate 24 Well
USB cable to connect the ibidi Temperature Controller – Silver
Line with a computer
Country-specific power cord to connect the external power sup-
ply to the wall socket
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ibidi Heating System Multiwell Plate – Silver Line
Description Drawing
External power supply for the ibidi Temperature Controller – Sil-
ver Line
USB flash drive with IncuControl software
Cable clip for optional use
4.2 Stage Top Incubator Overview
The parts of the ibidi Heating System Multiwell Plate are set up as a stage to incubator as shown in
Figure 5. The ibidi Heating System Multiwell Plate can be combined with the ibidi Gas Incubation
System – Silver Line in order to provide CO2and (optional) O2control and a defined humidified
atmosphere in addition to temperature control. Detailed information on the ibidi Gas Incubation
System – Silver Line is given in the Gas Incubation System instructions.
ibidi Temperature Controller
ibidi Gas Mixer with
Humidifying Column
Your inverted microscope*
Existing stage or frame with
K-frame fitting (160 x 110 mm2)
on your microscope*
ibidi Gas Mixer
Flow
I 9.9
S[ 10.0] Run
CO2% Hum%
5.1
5.0 75.2
80.0
Setup
1
3
4
6
25
ibidi Temperature Controller
1 Plate
I 38.0
S[ 38.0] Run
Lid
42.1
42.0 Setup
37.2
Heated Plate with
Heated Glass Bottom
Heated Lid
ibidi µ-Plate,
multiwell plate with a ANSI/SLAS (SBS)
standard format (85.5 x 127.5 mm2)
* Not part of the ibidi Stage Top Incubator.
Please contact us for information on suitable microscopes.
Figure 5: Overview of the parts of the ibidi Heating System Multiwell Plate, combined with the ibidi
Gas Incubation System for a full stage top incubator.
The Incubation Chamber Multiwell Plate fits on an inverted microscope stage equipped with a
mounting frame K (160 × 110 mm). The Incubation Chamber Multiwell Plate can hold all well-plates
matching the ANSI SLAS 1-2004 standard (R2012) with a maximum height of 23 mm.
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ibidi Heating System Multiwell Plate – Silver Line
4.3 ibidi Temperature Controller – Silver Line
The ibidi Temperature Controller – Silver Line is designed to precisely operate the different ibidi
Silver Line heating devices, including the Incubation Chamber Multiwell Plate – Silver Line. In total,
6 channels are available, each channel accommodating one heated component (e.g., the Heated Lid,
Heated Plate, and Heated Glass Bottom of the Incubation Chamber Multiwell Plate, which cover 3
channels). In addition, an external Temperature Sensor can be connected.
The front panel enables access to the Temperature Controller’s settings. The front panel display
shows the values of set (‘S’ = set) and actually measured (‘I’ = is) temperatures. The temperature set
values and other settings can be adjusted via the control buttons (Section 5.3).
If you wish to control the Temperature Controller via PC, use the IncuControl software (Section 6).
1
3
4
6
25
ibidiTemperature Controller
Plate
I 38.0
S[ 38.0] Run
Lid Glass
42.1 36.9
42.0 37.0
Setup
N.C.
1
Figure 6: Front view of the ibidi Temperature Controller – Silver Line.
The LEDs of the front panel indicate the status of the channels, connection to power supply, and USB
connection (Figure 6).
Control LEDs Channel 1–6
LED off Channel inactive
LED on Channel active
LED fast blinking Channel error
Please contact ibidi at [email protected].
All electrical connectors are located on the back of the Temperature Controller (Figure 7). For con-
necting the heating devices, two port sizes are available. The larger ports 1/2 and 4/5, which each
accommodate two output channels, and the smaller ports for the output channels 3 and 6. Please
find which heating units can be connected to which port in Table 4(Section 5.3). Refer to Section 5.1
for setting up the ibidi Heating System Multiwell Plate.
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ibidi Heating System Multiwell Plate – Silver Line
USB Power
supply
External
temperature
sensor
On/Off switch
Type:
Serial number:
Production date:
ibidi Temperature Controller
ibiTC3-001
11/2021
ibidi GmbH
Am Klopferspitz 19
D-82152 Martinsried
Germany
[phone]
www.ibidi.com
Temp
Sensor
PowerSignal
Out
1 / 2 3 64 / 5
Output Channels
24 V
max. 6.7 A
USB
Electrical cable
to Gas Mixer
Ports to
heating devices
On Off
Figure 7: Rear view of the ibidi Temperature Controller – Silver Line.
4.4 Heated Plate
The Heated Plate provides the base for the ibidi Heating System Multiwell Plate. The lower part of
the Heated Plate fits into a mechanical or scanning stage with opening for mounting frames K (160
mm × 110 mm).
Electrical connection
Temperature Controller “Heated Plate“
and „Heated Glass Bottom“
Heated Plate
Heated Glass Bottom
Set screw
Fixation of the
Heated Glass Bottom
Set screw
Set screw
Set screw
Figure 8: Heated Plate, top view.
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