ibidi 12170 User manual

12170 Objective Heater Universal – Silver Line
Instruction Manual
Objective Heater Universal – Silver Line
Version 1.0
for use with ibidi Heating System Slide / Dish – Silver Line
and ibidi Heating System 4 Slides – Silver Line
12110, 12720,
12722, 12111
12130, 12131
.com

Objective Heater Universal – Silver Line
Version 1.0 (June 1, 2022) 2 Instruction Manual

Objective Heater Universal – Silver Line
Contact
ibidi GmbH
Lochhamer Schlag 11
82166 Gr¨ afelfing
Germany
Phone: +49 89 / 520 46 17 - 0
Fax: +49 89 / 520 46 17 - 59
E-mail: [email protected]
Internet: ibidi.com
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Objective Heater Universal – Silver Line
Contents
1 Preamble 5
1.1 Introduction ........................................... 5
1.2 Introduction ........................................... 5
1.3 Safety Symbols ......................................... 5
1.4 Nomenclature .......................................... 6
1.5 Specifications .......................................... 6
1.6 Disclaimer ............................................ 7
1.7 Safety Considerations ...................................... 7
1.8 Limited Warranty ........................................ 8
1.9 Repairing the Objective Heater Universal – Silver Line .................. 9
1.10 Waste Disposal – WEEE/RoHS Compliance Statement .................. 9
1.10.1 EU Directive WEEE ................................... 9
1.10.2 EU Directive RoHS ................................... 10
1.11 Regulatory Statement ...................................... 11
2 Intended Use 12
3 Principle 12
4 Equipment 13
5 Operation 14
5.1 Installation and Connection of the Objective Heater Universal – Silver Line . . . . . . 14
5.2 Emergency Release Mechanism ................................ 16
5.3 Temperature Adjustment of the Objective Heater Universal – Silver Line ........ 16
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Objective Heater Universal – Silver Line
1 Preamble
1.1 Introduction
1.2 Introduction
This manual is your guide to using the Objective Heater Universal – Silver Line for cell culture ex-
periments on an optical microscope. It instructs first-time users on how to use the instrument, and
serves as a reference for experienced users.
Before using the Objective Heater Universal – Silver Line, please read this instruction manual care-
fully and make sure that the contents are fully understood. This manual should be easily accessible to
the operator at all times during instrument operation. If this manual gets lost, see the online version
on ibidi.com or request a replacement via [email protected].
To ensure safe operation, the Objective Heater Universal – Silver Line must only be operated with
the supplied components and according to the instruction manual.
For Research Use Only! Not for use in diagnostic procedures.
The Objective Heater Universal – Silver Line (#12170) can be used with the Heating System Slide/Dish
– Silver Line (product numbers 12110, 12720, 12722, 12111) and the Heating System 4 Slides – Silver
Line (product numbers 12130, 12131).
1.3 Safety Symbols
Note that the signal words WARNING,CAUTION and NOTE have specific meanings in this man-
ual. Do not proceed beyond a signal word until you have performed the indicated actions. Warning
messages in the text are displayed in a gray shaded box. Please see section ?? for general safety
considerations.
WARNING – A potentially hazardous situation which, if not avoided, could result
in serious injury or even death.
CAUTION – A potentially hazardous situation which, if not avoided, could result in
minor or moderate injury. It is also used to alert against damaging the equipment or
the instrument.
NOTE – Additional information to help achieve optimal instrument and assay per-
formance.
Symbols on the product identification label and back panel of the device:
CE Marking: This symbol indicates the product’s compliance with EU legislation.
This label is positioned on the back of the device and prompts you to read the manual
before using the device.
Product disposal: The symbol indicates that this product must be recycled/disposed
of separately from other household waste. See page 9for details.
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Objective Heater Universal – Silver Line
1.4 Nomenclature
For better readability, the Objective Heater Universal – Silver Line is hereafter abbreviated and re-
ferred to as “Objective Heater” in body text and figure captions. The Objective Heater consists of the
following three components:
Flexible heating unit Cable Velcro®fastener
1.5 Specifications
Table 1: Specifications of the Objective Heater Universal – Silver Line
Electrical Specifications
Overvoltage category III
Input line voltage DC 24 V, 2 A, 48 W
Operating Conditions
Operating site Indoor use only
Operating temperature 18–30°C/64–86°F (min 5°C/9°F less than set tempera-
ture)
Humidity 80% relative humidity (RH) up to 31°C/87°F
30% relative humidity (RH) up to 40°C/104°F
Operating altitude max. 2000 m (atmospheric pressure 800–1060 hPa/
11.6–15.4 psi)
Storage conditions -5–50°C/23–122°F, humidity <60% relative humidity
(RH)
Outer Dimensions and Characteristics
Length of flexible heating unit 12 cm
Height of flexible heating unit 2 cm
Length of cable 232.5 cm
Weight of heating unit including cable 110 g
Fit With Microscope Objectives
Diameter of Objectives ∼2.5–4.5 cm
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Objective Heater Universal – Silver Line
Table 1: (continued)
Compatibility With Temperature Controllers and Temperature Control Range
Compatibility ibidi Temperature Controller – Silver Line
Temperature control range Ambient temperature (min. 18°C/64°F) to 45°C/113°F
Recommended temperature settings See Section 5.3
NOTE – The Objective Heater is only compatible with the ibidi Temperature Controller –
Silver Line, hereafter abbreviated and referred to as ”Temperature Controller”.
1.6 Disclaimer
• ibidi shall not be held liable, either directly or indirectly, for any damage incurred as a result of
product use.
• The contents of this manual are subject to change without notice for product improvement.
• This manual is considered complete and accurate at publication.
• This manual does not guarantee the validity of any patent rights or other rights.
• If an ibidi software program doesn’t function properly, this may be caused by a conflict from
another program operating on the computer. In this case, take corrective action by uninstalling
the conflicting product(s).
• ibidi is a registered trademark of ibidi GmbH in Germany and other countries.
1.7 Safety Considerations
WARNING
• Only operate the Objective Heater with the supplied components and the Temperature Con-
troller.
• Only use the cables and plugs delivered with the Objective Heater. The power plug of the
control unit must be inserted in an outlet with a ground (earth) contact.
• Do not replace detachable power cables with inadequate specifications power cables. By vio-
lating these instructions you risk electric shock and fire.
• Do not operate the Objective Heater under conditions that pose a risk of explosion, implosion,
or the release of gases.
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Objective Heater Universal – Silver Line
• Do not place flammable solids, liquids, gases, or gas outlets near the Objective Heater (e.g.,
matches, ethanol, propane gas, solvents). Do not bring these products in contact with any other
component of the system either.
• Do not operate a damaged Objective Heater. If the Objective Heater seems to be damaged,
contact [email protected].
• Only operate the Objective Heater properly installed to a microscope objective.
• Some accessible parts of the Objective Heater can reach temperatures up to 65°C. Avoid touch-
ing the temperature-controlled parts of the system when you have set the Temperature Con-
troller to high temperatures.
CAUTION
• Not all objectives/lenses tolerate heat! Please check with the manufacturer of your objective if
it can be heated.
• Ensure that the external power supply is easily accessible. The Objective Heater must be in-
stalled in a manner such that none of its components hinders access to the external power
supply.
• Immediately replace damaged cords, plugs, or cables to avoid the risk of personal injury or
damage to the instrument.
• Only ibidi technical staff and technical staff instructed by ibidi are permitted to open and ser-
vice the Objective Heater.
• The Objective Heater should not come in contact with moisture. If the housing is damaged, the
external power supply should not be used.
• Avoid strong magnetic fields and sources of high frequency. The Objective Heater might not
function properly when located near a strong magnetic field or high frequency source.
• Avoid vibrations from vacuum pumps, centrifuges, electric motors, processing equipment, and
machine tools.
• Avoid dust and corrosive gas. Do not install the Objective Heater where it could be exposed to
high levels of dust or outside air or ventilation outlets.
• Install the Objective Heater in a location that enables easy access for maintenance.
• Do not place heavy objects on the Objective Heater and do not fold the Objective Heater.
1.8 Limited Warranty
Products manufactured by ibidi, unless otherwise specified, are warrantied for a period of one year
from the date of shipment to be free of defects in materials and workmanship. If any defects in the
product are found during this warranty period, ibidi will repair or replace the defective part(s) or
product free of charge.
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Objective Heater Universal – Silver Line
This warranty does not apply to defects resulting from the following:
1. Improper or inadequate installation.
2. Improper or inadequate operation, maintenance, adjustment, or calibration.
3. Unauthorized modification or misuse.
4. Use of consumables, disposables, and parts not supplied by an authorized ibidi distributor.
5. Corrosion due to the use of improper solvents, samples, or due to surrounding gases.
6. Accidents beyond ibidi’s control, including natural disasters.
This warranty does not cover consumables, such as cell culture chambers and dishes, tubes, fluidic
connectors, reagents, etc.
The warranty for all parts supplied and repairs provided under this warranty expires on the warranty
expiration date of the original product.
1.9 Repairing the Objective Heater Universal – Silver Line
For inquiries concerning repair service contact ibidi GmbH technical support ([email protected])
and provide the model name and serial number of your system.
CAUTION Do not try to repair the Objective Heater by yourself. Disassembly of the Objec-
tive Heater is not allowed. Disassembly poses a risk of personal injury or damage to the devices.
Contact ibidi technical support if there is a need to disassemble a device.
1.10 Waste Disposal – WEEE/RoHS Compliance Statement
The European Union (EU) has enacted two directives, the first on product recycling (Waste Electrical
and Electronic Equipment, WEEE) and the second on limiting the use of certain substances (Restric-
tion on the use of Hazardous Substances, RoHS).
1.10.1 EU Directive WEEE
The Objective Heater Universal – Silver Line must be disposed of in compliance with the WEEE
Directive 2012/19/EC.
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Objective Heater Universal – Silver Line
This symbol on the product is in accordance with the European Union’s Waste Electrical and Elec-
tronic Equipment (WEEE) Directive. The symbol indicates that this product must be recycled/dis-
posed of separately from other household waste. It is the end user’s responsibility to dispose of this
product by taking it to a designated WEEE collection facility for the proper collection and recycling of
the waste equipment. The separate collection and recycling of waste equipment will help to conserve
natural resources and protect human health and the environment. For more information about recy-
cling, please contact your local environmental office, an electrical/electronic waste disposal company
or distributor where you purchased the product.
1.10.2 EU Directive RoHS
RoHS conformity is declared in the EU-conformity in Section 1.11.
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Objective Heater Universal – Silver Line
1.11 Regulatory Statement
Diese Erklärung bescheinigt die Übereinstimmung mit den genannten Richtlinien,
ist jedoch keine Zusicherung von Eigenschaften. Die Sicherheitshinweise der
Produktdokumentation sind zu beachten.
This declaration certifies the conformity to he specified directives but not includes
any warranted quality of the instrument. The safety documentation of the product shall
be considered in detail
EG-Konformitätserklärung
EC Declaration of Conformity
Wir / We
ibidi GmbH
Lochhamer Schlag 11
D-82166 Gräfelfing
erklären hiermit die Übereinstimmung des genannten Produktes mit der Richtlinie 2014/35/EU - Niederspannungsrichtlinie und
mit der Richtlinie 2014/30/EU über die Elektromagnetische Verträglichkeit.
Bei Änderungen am Produkt, die nicht von uns autorisiert wurden, verliert diese Erklärung ihre Gültigkeit.
We declare the compliance of the product with the requirements of the Directive 2014/35/EU - Low Voltage Directive
and with the Directive 2014/30/EU about the Electromagnetic Compatibility.
Any modification to the product, not authorized by us, will invalidate this declaration.
Laborgerät / laboratory equipment:
ibidi Temperature Controller ibiTC3-XXX with ibidi accessories
Der oben beschriebene Gegenstand erfüllt die Vorschriften der Richtlinie 2011/65/EU vom 08. Juni 2011
zur Beschränkung der Verwendung bestimmter gefährlicher Stoffe in Elektro- und Elektronikgeräten.
The object of the declaration described above is in conformity with Directive 2011/65/EU of 8 June 2011
on the restriction of the use of certain hazardous substances in electrical and electronic equipment.
Das Produkt entspricht den unten aufgeführten Normen:
The product meets the requirements of the following standards:
Sicherheitsbestimmungen für elektrische Mess-, Steuer-, Regel- und Laborgeräte - Teil 1: Allgemeine Anforderungen Safety
requirements for electrical equipment for measurement, control and laboratory use - Part 1: General requirements
Elektrische Mess-, Steuer-, Regel und Laborgeräte. EMV-Anforderungen. Allgemeine Anforderungen
Electrical equipment for measurement, control and laboratory use. EMC requirements. General requirements
Das Produkt ist gekennzeichnet mit/ The product is marked with
Dr. Valentin Kahl
Geschäftsführer
Name, Funktion Unterschrift
Gräfelfing, den 29.07.2021
Gräfelfing, 2021-07-29
Ort/Datum
Place/date
Name, Function Signature
DIN EN 610
10-1:2020
DIN EN 61326-1:2013
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Objective Heater Universal – Silver Line
2 Intended Use
The Objective Heater Universal – Silver Line is designed for use in combination with a stage top
incubation system to keep cells on a microscope stage at 37°C. The Objective Heater, operated with
the ibidi Temperature Controller – Silver Line, is a heating device for microscope objectives used
for live cell imaging. Heating of the microscope objective with the Objective Heater prevents strong
temperature gradients between the sample and the objective. This is especially important for high
magnification objectives that are in close contact with the sample (e.g., water or oil immersion objec-
tives). Using the Objective Heater improves keeping a defined and stable temperature of the sample.
Please note that the Objective Heater is designed for research purposes only.
3 Principle
Microscope objectives that require close approximation to the sample (e.g., water and oil immersion
objectives) might cause cooling of the sample by thermal transfer from the sample to the objective,
when the objective is not heated. Adequate heating of the objective by the Objective Heater prevents
cooling of the sample. The Objective Heater includes a flexible heating element that can be easily
wrapped around microscope objectives and fixed by a Velcro®fastener to enable efficient heating by
a large contact area between the microscope objective and the Objective Heater.
Figure 1: Schematic illustration of a live cell imaging setup with an ibidi Stage Top Incubator and the
Objective Heater for optimal temperature stability of the sample.
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Objective Heater Universal – Silver Line
4 Equipment
This section gives an overview of the components and their functionality and characteristics.
The components of the Objective Heater Universal – Silver Line (#12170) are listed below.
Description Drawing
Flexible heating unit (red) with
piece of cable and part (female) of
the break-away connector
Cable for connecting the Objective Heater
to the Temperature Controller, with
part (male) of the break-away
connector, converter box and plug
Velcro®fastener for holding the
Objective Heater on the objective
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Objective Heater Universal – Silver Line
5 Operation
Before starting an experiment, check that the Objective Heater fits your microscope objectives (see
Section 1.5).
CAUTION Not all objectives/lenses tolerate heat! Please check with the manufacturer of
your objective if it can be heated.
NOTE – The Temperature Controller controls the temperature of the Objective Heater ac-
cording to the sensor inside the heating unit of the Objective Heater. As the Objective Heater
dissipates heat to the objective and the sample, the temperature of the Objective Heater is typ-
ically set higher than the target temperature of the sample. In order to adjust the temperature
within the sample to a specific target temperature follow the instructions in Section 5.3.
5.1 Installation and Connection of the Objective Heater Universal – Silver Line
Objectives come in different sizes and lengths, depending on manufacturer and magnification. Hence,
there are two different ways to mount the Objective Heater to a microscope objective (Figure 2).
1. Test if the heating unit creates a significant overlap when wrapped around the objective. If there
is no overlap, proceed with steps 2A and 3A. If there is an overlap, which is not optimal for homoge-
nous heating, proceed with steps 2B and 3B.
2A. Thread the blue end of the Velcro®fastener through the metal buckle and create a loose loop that
can be easily placed over the objective.
3A. Bend the heating unit of the Objective Heater around the objective, the cable directing towards
the revolving nosepiece (turret) of the microscope. Next, place the Velcro®fastener around the heat-
ing unit while holding it in place, and secure the Objective Heater in the desired position by closing
the Velcro®.
2B. Thread the blue end of the Velcro®fastener as well as one end of the heating unit carefully through
the metal buckle and create a loose loop that can be easily placed over the objective.
3B. Place the Objective Heater around the objective, the cable directing towards the revolving nose-
piece (turret) of the microscope. Fasten the Velcro®fastener and heating unit around the objective so
that there is enough contact between heating unit and objective. Close the Velcro®.
4. Connect the electrical cable of the Objective Heater to port 3 or 6 at the back of the Temperature
Controller.
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Objective Heater Universal – Silver Line
Figure 2: Mounting of the Objective Heater to an objective.
To control the temperature of the Objective Heater, please follow the instructions of the Temperature
Controller (see Instructions). Optional: To setup communication with the IncuControl Software, use
a USB cable to connect the Temperature Controller to the computer.
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Objective Heater Universal – Silver Line
5.2 Emergency Release Mechanism
A break-away connector in the cable between the Objective Heater and the Temperature Controller
allows for emergency release of the Objective Heater from the Temperature Controller (see Figure
3). If a force above about 14 N acts on the cable of the Objective Heater, the break-away connector
releases open to prevent any force from being transferred to the microscope’s objective.
Figure 3: Principle of the emergency release mechanism by a break-away connector in the cable
between the Objective Heater and the Temperature Controller.
5.3 Temperature Adjustment of the Objective Heater Universal – Silver Line
ibidi recommends a temperature adjustment before starting the first experiment to ensure that the
sample reaches the correct temperature. The temperature in the sample is affected primarily by the
temperature of the Stage Top Incubator. In addition, the temperature of the microscope objective
can contribute to the sample temperature by heat transfer from the sample to the objective and vice
versa. The temperature of the Objective Heater is regulated by the Temperature Controller. Please
follow the instructions for temperature adjustment of the sample as described in the instructions of
the Temperature Controller (see Instructions).
1. Adjust temperatures of Heated Plate and Heated Lid in a way that the target temperature for
the sample is reached (e.g., 37°C), while the objective is not yet in contact with the sample.
2. As soon as the target temperature of the sample is reached, bring the cold objective in contact
with the sample. The temperature of the sample will most likely drop. Wait until the tempera-
ture of the sample reaches a stable plateau.
3. In order to bring back the sample temperature to the target temperature, heat up the Objective
Heater to an initial temperature between 38°C and 45°C. The optimal temperature depends on
the diameter and material of your objective. Wait until the temperature is stable again.
4. Adjust the temperature of the Objective Heater, if necessary. We recommend beginning the
temperature adjustment with a low temperature and to increase the temperature stepwise by
0.5–1 °C.
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Objective Heater Universal – Silver Line
Figure 4: Temperature adjustment procedure to adjust the sample temperature to the target temper-
ature.
The temperature adjustment must be repeated from time to time (at least once a year), especially if
one of the following conditions has been changed:
• Room temperature
• Air conditioning
• Chamber type or objective lens
• Humidification and gas flow
• Use of an XL-incubator
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Objective Heater Universal – Silver Line
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Objective Heater Universal – Silver Line
Instruction Manual 19 Version 1.0 (June 1, 2022)

ibidi GmbH
Lochhamer Schlag 11
82166 Gräfelfing
Germany
Toll free within Germany:
Phone: [phone]
Fax: 0800 / 00 11 11 29
International calls:
Phone: +49 89 / 520 46 17 - 0
Fax: +49 89 / 520 46 17 - 59
E-Mail: [e-mail]
ibidi.com
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