ibidi Heating System User manual

Version 25.04.2017
Heating System
Instructions

Instructions
2 Heating System Version (26th April 2017)

Instructions
Contents
Safety Considerations 4
1 Principle – Physiological Conditions in Live Cell Imaging 15
2 Equipment 16
2.1 Components of the Heating System ............................. 16
2.2 Combination Options ...................................... 17
2.3 Temperature Controller ..................................... 18
2.4 Heated Lid ............................................ 20
2.5 Heated Plate ........................................... 20
2.6 Heating Inserts ......................................... 21
2.6.1 Heating Insert for µ–Slides .............................. 21
2.6.2 Heating Insert for µ–Dishes .............................. 22
3 Operating the Heating System 23
3.1 Installation and Connection of the Parts ........................... 23
3.2 Sample Preparation ....................................... 23
3.3 Start the Heating System .................................... 24
3.4 Setting Temperatures in the Control Panel .......................... 24
3.5 Temperature Adjustment in the Sample ........................... 25
4 Maintenance 27
4.1 Sterilization and Cleaning ................................... 27
4.2 Influence of Surrounding Temperature and Airflow .................... 27
5 Troubleshooting 28
5.1 Focus not Stable ......................................... 28
5.2 Evaporation is Too High .................................... 28
5.2.1 Silicone Oil Procedure (Anti-Evaporation Oil) ................... 28
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Instructions
Safety Considerations
To ensure operation safety, the Heating System must be operated correctly and maintained accord-
ing to a regular schedule. Carefully read all safety precautions in this manual before operating the
instrument. Note that the signal words WARNING,CAUTION and NOTE have specific meanings
in this manual.
Safety Symbols
Do not proceed beyond a WARNING or CAUTION notice until you have performed the indicated
actions.
WARNING! A potentially hazardous situation which, if not avoided, could result in
serious injury or even death.
CAUTION A potentially hazardous situation which, if not avoided, could result
in minor or moderate injury. It is also used to alert against damaging
the equipment or the instrument.
NOTE Additional information to help achieve optimal instrument and assay
performance.
This label is positioned on the back of the device and prompts you to
read the manual before using the device.
Warning message
Warning messages in the text are displayed in a gray shaded box.
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Instructions
Nomenclature
Temperature Controller
Heated Lid
Heated Plate
WARNING!
• Do only operate the Heating Devices with the supplied Temperature Controller.
• Do only use the delivered cables and plugs. If not doing so you risk electric shock and fire.
• Do not operate the Heating System with substances or under conditions, that can cause a risk
of explosion, implosion or the release of gases.
CAUTION
• Ensure that the external power supply is well accessible. The Heating System must be installed
in such manner that none of its components hinders access to the external power supply.
• The weight of the Temperature Controller is approx. 3 kg. Moving the Temperature Controller
during operation will pose a risk of personal injury or damage to the instrument.
• Some accessible parts of the Heated Plate and Heated Lid can reach temperatures up to 55°C.
Avoid touching the temperature-controlled parts of the system when you have set the Temper-
ature Controller to high temperatures.
• The glass plate of the Heated Lid can break due to a mechanical impact. If so, the shards can
lead to injuries if handled.
• Be aware that when the system is switched on, 10 V AC voltage is applied to the underside of
the glass on the Heated Lid and bottom of the Heated Plate. Do not touch the underside or put
it in contact with anything conductive. This could cause a short circuit that may destroy the
Temperature Controller and/or the Heating Devices.
• Only use the Heating System in dry rooms.
• Do not use the Heating System in cold rooms.
• Use the Heating System only for cell experiments with aqueous solutions.
• Do not use the Heating System with hazardous substances or substances/materials that pose a
risk of infections.
• Do not use extension cords. Immediately replace damaged cords, plugs, or cables to avoid a
risk of personal injury or damage to the instrument.
• Only ibidi technical staff is permitted to open and service the Heating Devices and/or Temper-
ature Controller.
• Unplug the external power supply when the Heating System is not in use.
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Instructions
Regulatory Statement
The following safety and electromagnetic standards were con-
sidered:
• IEC 61010-1:2010 + Cor. :2011 Safety requirements for
electrical equipment for measurement, control and labo-
ratory use - Part 1: General requirements
• IEC 61326-1:2006 EMC, Electrical equipment for measure-
ment, control and laboratory use. EMC requirements.
General requirements
• IEC/CISPR 11:2009, modified + A1:2010, Industrial, sci-
entific and medical equipment - Radio-frequency distur-
bance characteristics - Limits and methods of measure-
ment
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Instructions
Specifications
External Power Supply:
Heated Lid: AC 10 V, 50-60 Hz
Heated Plate: AC 24 V, 50-60 Hz
Temperature Controller: AC 230 V/50 Hz or 115 V/60 Hz
Operating Conditions
Environmental operating temperature: 18-30°C (indoor use only)
Storage temperature: -5-50°C, humidity up to 80% RH
Humidity: 80% RH up to 30°C
Operating altitude: max. 2000 m (atmospheric pressure 800-1060 hPa)
Dimensions of Heated Plate with Lid
Width: 125.5 mm
Depth: 85.5 mm
Height: 25.5 mm
Weight: 210 g
Temperature Control Range
Heated Lid (Channel 1): Ambient temperature (min. 18°C) to +46°C
Heated Plate (Channel 2): Ambient temperature (min. 18°C) to +45°C
Recommended Temperatures before Adjustment
(see Section3.5)
Heated Lid (Channel 1): 42°C
Heated Plate (Channel 2): 37°C
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Instructions
Compatibility
The parts of the Heating System must only be used according to the instructions. The compatibility
of all parts is shown in detail in section 2.2.
Notices
1. ibidi shall not be held liable, either directly or indirectly, for any consequential damage incurred
as a result of product use.
2. Prohibitions on the use of ibidi software
• Copying software for other than backup purposes
• Transfering or licensing of the right to use software to a third party
• Disclosure of confidential information regarding software
• Modification of software
• Use of software on multiple workstations, network terminals, or by other methods
3. The contents of this manual are subject to change without notice for product improvement.
4. This manual is considered complete and accurate at publication.
5. This manual does not guarantee the validity of any patent rights or other rights.
6. If an ibidi software program has failed causing an error or improper operation, this may be
caused by a conflict from another program operating on the notebook (PC). In this case, take
corrective action by uninstalling the conflicting product(s).
7. ibidi is a registered trademark of ibidi GmbH in Germany and other countries.
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Instructions
Limited Warranty
Products sold by ibidi, unless otherwise specified, are warranted for a period of one year from the
date of shipment to be free of defects in materials and workmanship. If any defects in the product are
found during this warranty period, ibidi will repair or replace the defective part(s) or product free of
charge.
This warranty does not apply to defects resulting from the following:
1. Improper or inadequate installation.
2. Improper or inadequate operation, maintenance, adjustment or calibration.
3. Unauthorized modification or misuse.
4. Use of unauthorized tubing or fluidic connectors.
5. Use of consumables, disposables and parts not supplied by an authorized ibidi distribu-
tor.
6. Corrosion due to the use of improper solvents, samples, or due to surrounding gases.
7. Accidents beyond ibidi’s control, including natural disasters.
This warranty does not cover consumables, such as cell culture chambers and dishes, tubes, fluidic
connectors, reagents etc.
The warranty for all parts supplied and repairs provided under this warranty expires on the warranty
expiration date of the original product. For inquiries concerning repair service, contact ibidi and
provide the model name and serial number of your ibidi System.
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Instructions
Installation Requirements
To ensure operation safety and optimal performance, meet the following conditions:
1. Only operate the ibidi Heating Devices with an ibidi Temperature Controller and with the sup-
plied power cable.
2. Ensure that the power plug of the power supply is well accessible. The Heating Devices must
be installed in a manner that they do not hinder the access to the external power supply and its
power plug.
3. Only operate the Heated Plate with original ibidi inserts.
4. Operate the Heating System in a temperature range of 18-30°C.
5. Operate the Heating System in a humidity range of up to 80% RH.
6. Operate the Heating System in an atmospheric pressure range of 800-1060 hPa.
7. Do not operate the Heating System under conditions that pose a risk of explosion, implosion,
or the release of gases.
8. Avoid strong magnetic fields and sources of high frequency. The Heating System might not
function properly when located near a strong magnetic field or high frequency source.
9. Avoid vibrations from vacuum pumps, centrifuges, electric motors, processing equipment, and
machine tools.
10. Avoid dust and corrosive gas. Do not install the Heating System where it could be exposed to
high levels of dust or to outside air or ventilation outlets.
11. Do not install the Heating System in a location where it could be exposed to direct sunlight.
12. Install the Heating System in a horizontal and stable position, which includes a table, bench or
desk upon which the instrument is installed.
13. Install the Heating System in a location that enables easy access for maintenance.
14. Do not place heavy objects on the instrument.
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Instructions
Installing and Connecting Cables
Confirm that the power switch of the Temperature Controller is off (power switch is at the right, rear
side of the Temperature Controller).
Connect the Heating Devices to the connectors at the rear of the Temperature Controller. Connect
each device to the connector labeled with the corresponding device name.
WARNING! To avoid risk electric shock and fire, operate the Heating System with the supplied
external power supply and the supplied cables and plugs.
CAUTION Ensure that the power supply is easily accessible. The Heating System must be installed
in a manner that does not hinder access to the power supply.
Connect the Temperature Controller to a Notebook by using the supplied network cable (only neces-
sary when using a PC to control the Heating System).
Switch on the Temperature Controller and the Notebook.
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Instructions
Maintenance and Operation
Keep the operating environment according to the installation requirements to maintain the declared
specifications of the Heating System.
Operating the Heating System
Use the Heating System only as described in this manual.
CAUTION Unintended use which is not in compliance with this manual, can lead to a failure of the
devices’ protective systems. This failure may cause personal injury or damage.
Cleaning the Heating System
Switch off the Temperature Controller and disconnect the Heating Devices from the controller. To
clean the Heating Devices and/or the Temperature Controller, use only dry cloth or a cloth dampened
with water (ultrapure) or common lab disinfection solutions that are based on quaternary ammonium
compounds (e.g., Barrycidal 36 or Pharmacidal).
CAUTION The use of ethanol or other types of organic solvents may remove the instrument’s paint.
Transporting the Heating System
First, switch off the Temperature Controller and then disconnect the Heating Devices from the con-
troller. Carry the devices carefully and avoid mechanical shocks.
Repairing the Heating System
Do not try to repair the Stage Top Incubator or the Temperature Controller by yourself. Contact the
ibidi service to repair any damaged components.
CAUTION The manual disassembling of the Heating System is not allowed. Manual disassembling
poses a risk of personal injury or damage to the devices. Contact ibidi service personnel if there is
need to disassemble the devices.
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Instructions
Waste Treatment – WEEE/RoHS Compliance Statement
Waste treatment lies in the responsibility of the user. Contact your institution’s waste management
department or a company specializing in waste recovery. Do not dispose the Heating System in a
trash bin or at a public waste disposable site. For detailed information please contact the ibidi service
department.
EU Directives WEEE and RoHS
To Our Valued Customers: ibidi is committed to being a good corporate citizen. As part of that
commitment, we strive to maintain an environmentally conscious manufacturing operation. The
European Union (EU) has enacted two directives, the first on product recycling (Waste Electrical and
Electronic Equipment, WEEE) and the second on limiting the use of certain substances (Restriction
on the use of Hazardous Substances, RoHS).
Two Categories of products covered by the WEEE Directive are currently exempt from the RoHS
Directive – Category 8, medical devices (with the exception of implanted or infected products) and
Category 9, monitoring and control instruments.
All of our products fall into either Category 8 or 9, and are currently exempt from the RoHS Direc-
tive. We will, however, continue to monitor the application of the RoHS Directive in relation to our
products and will comply with any changes, as they apply.
Recycling is offered for our products that fall within the scope of the WEEE Directive. These specific
products, available for sale after August 13, 2005, will be identified with a ”wheelie bin” symbol.
When you see this symbol, do not dispose of this product with Municipal Waste. Special Collec-
tion/Disposal is required. Please contact us for feedback or further information.
ibidi GmbH
Am Klopferspitz 19
82152 Martinsried
Germany
www.ibidi.com
[email protected]
Phone: [phone]
Fax: [phone]
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Preface
This manual is your guide to using the Heating System for cell culture experiments on an optical
microscope. It instructs first-time users how to use the instrument, and serves as a reference for
experienced users.
Before using the Heating System, please read this instruction manual carefully, and make sure that
the contents are fully understood. This manual should be easily accessible to the operator at all times
during instrument operation. When not using the Heating System, keep this manual in a safe place.
If this manual gets lost, order a replacement from the company website.

Instructions
1 Principle – Physiological Conditions in Live Cell Imaging
The Heated Lid and Heated Plate are designed to keep cells on–stage at 37°C in microscopy slides
and dishes (Figure 1). The Heated Lid prevents condensation effects inside the entire incubation
system (Figure 2).
The Heated Plate provides outer dimensions of 127.5 mm × 85.5 mm which fits perfectly into an
universal mounting frame K. The glass top of the Heated Lid and the Heated Plate are actively heat–
controlled by the Temperature Controller.
The modular concept of the Heating Inserts enables the use of different consumables ranging from
60 mm and 35 mm dishes to regular slides.
Combined with a Gas Mixer. humidity, CO2–enriched, and/or O2–reduced air can be flushed in,
which makes the incubation chamber a fully controlled incubator – right on the microscope stage.
Figure 1: Schematic view of the Heating System.
Figure 2: The Heated Lid prevents condensation effects on the lid of the cell culture vessel.
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Instructions
2 Equipment
The Heating System is a stage top incubator to keep samples on the microscope stage at the right
temperature. It consists of the Temperature Controller, Heated Lid, Heated Plate, and Inserts to hold
the samples.
The Heated Plate fits into a universal mounting frame for multiwell plates (outer dimensions 127.5 mm
× 85.5 mm). The complete height of the assembled Heated Lid and Heated Plate is 25.5 mm.
This section provides a brief overview including the nomenclature for later descriptions in this doc-
ument.
2.1 Components of the Heating System
The components of the Heating System are listed with a description and the short name that is used
in this instruction manual.
Description Short name Drawing
Temperature Controller Temperature
Controller
Heated Lid with electrical cable
and D-sub connector to connect to
the Temperature Controller
Heated Lid
Heated Plate with electrical cable
and D-sub connector to connect to
the Temperature Controller
Heated Plate
Heating Insert for µ–Dish 35mm, high
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Instructions
Description Short name Drawing
Heating Insert for µ–Slides.
1 temperature sensor
1 perforated ibidi µ–Slide 8 Well
1 perforated ibidi µ–Dish 35mm, high
1 perforated ibidi µ–Dish 35mm, low
Temperature Ad-
justment Set
Power cable for the Temperature
Controller Power cable
USB cable to connect the Tempera-
ture Controller with a computer USB cable
2.2 Combination Options
The parts of the Heating System are combined as shown in Figure 3. The Heated Lid and Heated
Plate fit on a microscope stage equipped with a universal mounting frame for multiwell plates. They
are connected to the Temperature Controller.
The Heating System can be combined with the Gas Mixer unit, that provides CO2and O2(optional)
control and a defined humidified atmosphere. Detailed information on the Gas mixer unit is given
in the Gas Mixer instructions.
To hold several geometries of Slides and Dishes, the Heated Plate can be equipped with different
Inserts (Section 2.6): Insert for µ–Dish 35mm, high, Insert for µ–Dish 35mm, low and Insert for µ–Slides.
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Instructions
Figure 3: Overview of the parts of the Heating System with options to combine
2.3 Temperature Controller
The Temperature Controller is designed to precisely operate the different heating devices. To achieve
this, the controller has four control channels that control the Heated Lid, Heated Stage (Insert), and
two optional channels. In addition, this controller can be run either as a stand–alone system or
PC–controlled. The display shows the measured (’I’ = is) and set (’S’ = set) temperatures of all the
channels. You can navigate through the menu by using the control buttons on the right. The LEDs
on the front indicate the status and feedback of the channels, connection to the power supply, and
USB connection (Figure 4).
Control LEDs
LED off Channel inactive
LED on/blinking slowly Channel active
LED blinking fast Channel error
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Instructions
Figure 4: Front view of the Temperature Controller.
All plugs for the electrical connections are integrated into the rear of the Temperature Controller
(Figure 5). The setup of the connections is shown in Section 11.
The available connections are:
• Power supply
• USB to connect to the computer
• 4 control channels
• External thermosensor (Type KTY-81)
• On/Off switch
Figure 5: Rear view of the Temperature Controller.
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Instructions
Supply Voltage Adjustments
The temperature controller must be adjusted to the corresponding voltage (230 V or 115 V). The
actual voltage setting may be read and adjusted at the integrated fuse box of the power cable
connector, located on the rear panel. In order to change these settings, it is necessary to pull out
the fuse holder from the casing. Replace the fuses to match the input voltage (1 A (T) for 230V /
50Hz or 2 A (T) for 115 V / 60 Hz). Rotate the grey fuse holder, and then reinsert it so that the
correct voltage is shown in the window. Snap the fuse box back into the housing. The ”On/Off”
switch is at the rear side of the controller. After selecting ”On”, the controller will automatically
start a self-test, and then the measured values will be shown.
2.4 Heated Lid
The Heated Lid (127.5 mm × 85.5 mm) provides excellent optical quality, and also allows for the use
of all standard microscopy techniques, including differential interference contrast (DIC). Due to the
height of the lid, we recommend using condensers with a working distance of ≥26 mm.
The upper glass part of the Heated Lid is electrically heat controlled. The electrical cable (1.5 m) is
connected to the Temperature Controller. The Heated Lid fits exactly and securely onto the Heated
Plate.
For gas incubation, the Heated Lid is equipped with inlets for the gas flow and the humidity sensor.
Detailed information on the Gas mixer unit is given in the Gas Mixer instructions.
Figure 6: Heated Lid
2.5 Heated Plate
The Heated Plate provides the base for the Heating System. The lower part of the Heated Plate fits
into any microscope stage with a universal mounting frame for multiwell plates. The electrical cable
(1.5 m) is connected to the Temperature Controller.
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