Infors HT Labfors 5 User manual

Operating Manual
2019-01-22
Labfors 5
Bench-Top Bioreactor - Version for Solid Substrates and Enzymatic Bioprocesses
Dok-ID: 77767 / V.02.00 - Original

Infors AG
Headoffice, Schweiz
Rittergasse 27
CH-4103 Bottmingen
T [phone]
F [phone]
[e-mail]
[e-mail]
Infors GmbH
Dachauer Str. 6
D-85254 Einsbach
T [phone]
F [phone]
[e-mail]
Infors UK Ltd
The Courtyard Business Centre
Dovers Farm, Lonesome Lane,
Reigate
Surrey, RH2 7QT, UK
T [phone]
F [phone]
[e-mail]
Infors Sarl
2, rue du Buisson aux Fraises
Bâtiment D13
F-91300 Massy
T [phone]
F [phone]
[e-mail]
www.infors-ht.fr
Infors Benelux BV
Markweg 9-A, NL-6883 JL
Velp (GLD)
P.O. Box 125, NL-6880 AC
Velp (GLD)
T [phone]
F [phone]
[e-mail]
Infors USA Inc.
9070 Junction Drive, Suite D
Annapolis Junction, MD20701
T [phone] /
T [phone] (toll-free USA)
F [phone]
[e-mail]
www.infors-ht.us
Infors Canada
8350 rue Bombardier
Anjou, Quebec
Canada H1J 1A6
T [phone]
F [phone]
[e-mail]
Infors Bio-Technology
(Beijing) Co., Ltd.
Room 505C, Building 106
Lize Zhongyuan
Wangjing New Industrial Zone
Chaoyang District, Beijing
100102 P.R. of China
T [phone]
F [phone]
[e-mail]
Infors South East Asia
16, 1st Floor, Taman City
MY-51200 Kuala Lumpur
Malaysia
T [phone]
F [phone]
[e-mail]
Infors LATAM Ltda.
Rua Dr. Alceu de Campos
Conjunto 205
CEP: 04544-000
São Paulo – SP
Brasil
T [phone]
F [phone]
[e-mail]
Contact details of our local dealers worldwide can be found
on our website.
www.infors-ht.com
Engineering and production in Switzerland

Labfors 5 – Version for Solid Substrates and Enzymatic Bioprocesses
Table of Contents
22 January 2019 Page 1of 180
1General Information ...............................................................8
1.1 About this Manual ..........................................................8
1.2 Explanation of Special Notices ......................................8
1.2.1 Warning Notices .............................................8
1.2.2 Other Notices..................................................9
1.3 Equipment Identification (Standard Identification Plate) 9
1.4 Declaration of Conformity ............................................10
1.5 Customer Service and Services ..................................10
2Safety and Responsibility ...................................................11
2.1 Intended Use, Incorrect Use and Misuse ....................11
2.2 Qualified Personnel .....................................................12
2.2.1 Provider ........................................................12
2.2.2 User ..............................................................12
2.2.3 Operator .......................................................13
2.3 Unauthorised Persons .................................................14
2.4 Responsibility of the Provider ......................................14
2.5 General Hazards..........................................................14
2.5.1 Electrical Current..........................................15
2.5.2Unauthorised Spare Parts and Accessories 15
2.6 Particular Hazards .......................................................15
2.6.1 Hot Surfaces.................................................16
2.6.2 Dangerous Gases ........................................16
2.6.3 Flammable or Explosive Substances ...........16
2.6.4 Corrosive or Toxic Substances ....................16
2.6.5 Bioactive Substances or Pathogenic
Organisms ....................................................17
2.6.6 Overpressure or Vacuum .............................17
2.7 Warning Symbols on the Equipment ...........................17
2.8 Declaration of Decontamination...................................18
3Setup and Function..............................................................19
3.1 Basic Unit.....................................................................19
3.1.1 Main Switch ..................................................20
3.1.2 Pumps ..........................................................20
3.1.3 Identification Plate ........................................21
3.1.4 Mains Connection.........................................21
3.1.5 Water Connections .......................................22
3.1.6 Gas Connections ..........................................22
3.1.7 Signal Connections ......................................23
3.1.8 Connections for Vessel ................................24
3.1.9 Gassing Connection (Sparger/Head Space) 25

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3.1.10 Sensor Connections (Sensor Cables) ..........25
3.1.11 Connections and Valve for Water Flow
Regulation of the Exit Gas Cooler ................26
3.2 Operating Panel ...........................................................27
3.2.1 Monitor Keys.................................................27
3.2.2 Operating Panel Connections.......................28
3.3 Culture Vessel..............................................................29
3.3.1 Top Plate ......................................................30
3.3.2 Ports in the Vessel Top Plate and their
Configuration ................................................31
3.4 Temperature Control System.......................................32
3.5 Stirrer ...........................................................................32
3.6 Gassing System ...........................................................35
3.6.1 Gasmix System.............................................35
3.6.2 Gas Entry......................................................35
3.7 Exit Gas........................................................................35
3.8 pH Control ....................................................................36
3.8.1 Measurement System...................................36
3.8.2 Calibration.....................................................37
3.8.3 Mounting of the Sensor ................................37
3.9 pO2Control...................................................................37
3.9.1 Measuring System ........................................38
3.9.2 Measurement and Calibration ......................39
3.9.3 Mounting of the Sensor ................................39
4Options..................................................................................39
4.1 Pump(s)........................................................................39
4.2 Servomotor (High Torque) ...........................................40
4.3 Exit Gas Analysis .........................................................42
4.3.1 Measuring Systems (Gas Sensors)..............42
4.3.2 Connecting the Gas Sensors........................42
4.3.3 Calibration.....................................................44
4.3.4 Replacing the BlueVary Gas Sensor Cartridge
......................................................................44
4.4 Multiplexer (Gas Switching Module) ............................44
4.5 Permissive Measurement ............................................45
4.6Pressure Control ..........................................................45
5Accessories ..........................................................................49
5.1 Base Tray.....................................................................49
5.2 Reagent Bottle and Pump Holder ................................50
5.3 Sampling System Super Safe Sampler........................51
5.4 Sparger with Sterile Sheath .........................................53
5.5 Impellers.......................................................................55

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5.6 Pocket for Temperature Sensor (Pt100)......................56
5.7 Blanking Plugs .............................................................56
5.8 Addition Port Adapters .................................................58
5.9 Inoculation Needles .....................................................59
5.10 Septum Collars ............................................................62
5.11 Probe Protection for pH Sensor...................................63
5.12 Dip Tubes.....................................................................64
5.13 Clamping Adapters ......................................................66
5.14 Electrode Holder ..........................................................67
5.15 Threaded Adapter ........................................................68
5.16 Adapter ........................................................................68
5.17 Exit Gas Cooler............................................................69
5.18 Reagent Bottles ...........................................................71
5.19 Pump Heads ................................................................73
5.20 Sterile Filters ................................................................73
5.21 O-Rings and Gaskets ..................................................75
5.22 Hoses and Accessories ...............................................75
5.23 Inoculation Accessories and Tools ..............................76
6Transport and Storage ........................................................77
6.1 Transport......................................................................77
6.2 Storage ........................................................................78
7Installation and Initial Operation ........................................79
7.1 General Location Requirements for Installation ..........79
7.2 Minimum Distances .....................................................80
7.3 Power Supply...............................................................80
7.4 Water Supply and Return ............................................80
7.5 Gas Supply ..................................................................81
7.6 Exit Gas .......................................................................82
7.7 Test Run ......................................................................82
7.7.1 Preparation Test Run ...................................82
7.7.2 Filling the Water Circuit ................................84
7.7.3 Stirring ..........................................................85
7.7.4 Heating and Adjusting Temperature ............85
7.7.5 Gassing ........................................................86
7.7.6 End of Test ...................................................86
8Before Fermentation/Cultivation ........................................88
8.1 Preparing and Autoclaving the Culture Vessel ............88
8.1.1 Checking Gaskets (O-Rings)........................88
8.1.2 Mounting the Impellers .................................89
8.1.3 Assembling and Mounting the Sparger ........90

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8.1.4 Moistening/Filling the Culture Vessel ...........92
8.1.5 Fitting the Vessel Top Plate..........................93
8.1.6 Mounting a Threaded Adapter......................94
8.1.7 Mounting the Blanking Plugs ........................95
8.1.8 Mounting Addition Port Adapters..................97
8.1.9 Mounting the Pocket for Temperature Sensor
(Pt100) ..........................................................98
8.1.10 Preparing an Inoculation Needle ..................98
8.1.11 Equipping Port with Septum Collar and
Septum for Inoculation..................................99
8.1.12 Mounting and Preparing the Exit Gas Cooler
......................................................................99
8.1.13 Checking Lubrication of the Mechanical Seal
....................................................................101
8.1.14 Preparing the Sensors................................101
8.1.14.1 Calibrating the pH Sensor .......... 102
8.1.14.2 Fitting a pH Sensor with a Probe
Protection ................................... 103
8.1.14.3 Mounting Sensors with Electrode
Holder......................................... 103
8.1.15 Preparing the Super Safe Sampler.............105
8.1.16 Mounting the Sparger Hose and the Inlet Air
Filter ............................................................107
8.1.17 Mounting the Hose and Inlet Air Filter for
Head Space Gassing..................................107
8.1.18 Preparing the Gassing Hose Line on the Basic
Unit .............................................................108
8.1.19 Calibrating the Pumps ................................109
8.1.20 Preparing the Reagent Bottles, Pumps and
Hoses..........................................................109
8.1.21 Sterile Hose Connections ...........................112
8.1.22 Removing the Pump Heads........................112
8.1.23 Checklist Before Autoclaving......................113
8.1.24 Autoclaving .................................................115
8.2 Connecting the Culture Vessel and Preparing the
Cultivation ..................................................................116
8.2.1 Connecting the Culture Vessel ...................116
8.2.2 Mounting the Pump Heads .........................117
8.2.3 Filling the Reagent Hoses ..........................117
8.2.4 Connecting the Gassing .............................118
8.2.5 Connecting the Exit Gas Cooler .................119
8.2.6 Coupling the Motor .....................................120
8.2.7 Filling the Culture Vessel............................120
8.2.8 Inserting the Temperature Sensor (Pt100) into
the Pocket...................................................121

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8.2.9 Connecting the pH Sensor .........................122
8.2.10 Connecting the pO2Sensor........................123
8.2.11 Polarising the pO2Sensor (METTLER)......124
8.2.12 Calibrating the pO2Sensor.........................124
8.2.13 Checking the Hoses and Hose Connections
....................................................................125
9Fermentation/Cultivation...................................................126
9.1 Preparing the Medium ...............................................126
9.2Sampling ....................................................................127
9.3 Inoculation..................................................................131
9.3.1 Inoculation with Inoculation Needle............132
9.3.2 Inoculation with a Syringe ..........................132
9.3.3 Inoculation Using Dip Tube / Addition Port
Adapter .......................................................133
9.4 Extending the Sparger with the Sterile Sheath..........133
9.5 Harvest.......................................................................134
9.6 Emptying the Culture Vessel .....................................135
9.7 Emptying the Reagent Hoses ....................................135
9.8 Switching off the Equipment ......................................135
9.9 Autoclaving the Culture Vessel after
Fermentation/Cultivation............................................136
10 Cleaning and Maintenance................................................138
10.1 Cleaning Agent and Disinfectant ...............................138
10.2 Cleaning the Culture Vessel - Routine Cleaning .......138
10.3 Removing the Vessel Top Plate and Accessories.....140
10.3.1 Removing the Exit Gas Cooler ...................140
10.3.2 Removing the Sensors ...............................140
10.3.3 Removing Hoses, Filters and Pump Heads
....................................................................141
10.3.4 Removing Blanking Plugs ..........................142
10.3.5 Removing Threaded Adapters ...................142
10.3.6 Removing the Inoculation Needle, Septum
Collar and Septum......................................142
10.3.7 Removing Addition Port Adapters ..............143
10.3.8 Removing the Vessel Top Plate .................143
10.3.9 Removing the Pocket for Temperature Sensor
(Pt100)........................................................145
10.3.10 Removing the Sparger and the Dip Tube(s)
....................................................................145
10.3.11 Removing the Impellers..............................147
10.3.12 Removing the Stirrer Shaft .........................147
10.4 Cleaning and Storing Individual Parts .......................148
10.5 Cleaning the Sensors ................................................149

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10.6 Cleaning the Hoses and Pump Heads.......................150
10.7 Cleaning the Super Safe Sampler .............................150
10.8 Cleaning the Exit Gas Cooler.....................................151
10.9 Cleaning the Basic Unit and Operating Panel ...........151
10.10 Maintenance Plan ......................................................151
10.11 Lubricating the Mechanical Seal ................................153
10.12 Decalcifying the Equipment .......................................153
10.13 Decalcifying Equipment via Vessel Jacket ................155
11 Interferences.......................................................................156
11.1 Interferences Basic Unit and Operating Panel...........156
11.2 Drive System Faults ...................................................157
11.3 Interferences Temperature Control System...............157
11.4 Interferences Gassing System...................................159
11.5 Interferences pH-System ...........................................160
11.6 Interferences pO2 System ..........................................161
11.7 Interferences Feed and Pump ...................................162
11.8 Returning for Repair...................................................163
12 Disassembly and Disposal ................................................163
12.1 Disassembly...............................................................163
12.2 Disposal .....................................................................164
13 Technical Data ....................................................................165
13.1 Dimensions 1 Unit ......................................................165
13.2 Dimensions Master Unit and Satellite Units...............167
13.3 Dimensions of Culture Vessels in Vessel Holder.......168
13.4 Weights (net)..............................................................170
13.5 Connection Values .....................................................170
13.5.1 Electrical .....................................................170
13.5.2 Water IN......................................................170
13.5.3 Water OUT..................................................171
13.5.4 Gas(es) IN ..................................................171
13.5.5 Exit Gas ......................................................171
13.6 Specifications .............................................................171
13.6.1 Operating Unit.............................................171
13.6.2 Culture Vessel ............................................172
13.7 Stirrer .........................................................................172
13.7.1 Impellers .....................................................173
13.7.2 Temperature ...............................................175
13.7.3 Gassing.......................................................176
13.7.4 pH ...............................................................176
13.7.5 pO2..............................................................177

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13.7.6 Pumps ........................................................178
13.7.7 Sterile Filters...............................................179
13.8 Operating Conditions .................................................179
13.9 Emissions...................................................................179
13.10 Utilities .......................................................................179
13.11 Auxiliary Supplies ......................................................180

Labfors 5 – Version for Solid Substrates and Enzymatic Bioprocesses
General Information
Page 8of 180 22 January 2019
1 General Information
1.1 About this Manual
This manual enables the safe and efficient handling of the equip-
ment.
All the information and instructions in this operating manual comply
with the current standards, legal regulations, the latest technologi-
cal and scientific developments and the knowledge gained from the
manufacturer’s many years of experience in this field.
This operating manual is a component part of the equipment.
It must be kept near to the equipment and must be accessible
to the operators at all times.
The users must read the operating manual thoroughly and fully un-
derstand its contents before beginning any work.
Adhering to all the safety and operating instructions in this manual
is essential to ensure that work is carried out safely.
The scope of delivery may differ from the explanations, descrip-
tions and figures in this operating manual due to special designs,
additional options specified on ordering and the latest tech-
nical/mechanical modifications.
This manual contains illustrations to aid general understanding.
These may differ from the actual equipment as supplied.
1.2 Explanation of Special Notices
1.2.1 Warning Notices
Warning notices in this manual are indicated by a coloured bar and
begin with a signal word that signifies the degree of the hazard.
DANGER
The signal word “DANGER” indicates a dangerous situation that
will lead to severe or even fatal injuries if not avoided.

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General Information
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WARNING
The signal word “WARNING” indicates a potentially dangerous
situation that may result in severe or even fatal injuries if not
avoided.
CAUTION
The signal word “CAUTION” indicates a potentially dangerous
situation that may result in minor injuries if not avoided.
1.2.2 Other Notices
ATTENTION
The word “ATTENTION” on a blue bar indicates a situation that
may result in significant damage to property if not avoided.
INFORMATION
Texts located below a grey bar bearing the notice “INFOR-
MATION” provide useful tips and recommendations for ensuring
efficient, fault-free operation of the equipment.
1.3 Equipment Identification (Standard Identification Plate)
The identification plate is designed to allow clear identification of
the equipment. It contains the following information:
Manufacturer name
Designation = Category of equipment
Type = Equipment type (name)
S/N = Serial number
Year = Year of manufacture
Mains = Nominal voltage and frequency
Current = Current consumption
Manufacturer address
CE marking

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1.4 Declaration of Conformity
The equipment is in compliance with the essential requirements of
the following Directives:
Machinery Directive 2006/42/EC
EMC Directive 2014/30/EU
The Declaration of Conformity according to EC Machinery Directive
2006/42/EC, annex II 1 A is included in the general documentation
supplied with the equipment.
1.5 Customer Service and Services
Our Customer Service is at your disposal for technical advice and
specialist enquiries. For contact information, see page 2.
Due to their familiarity with the potential applications of the equip-
ment, the Customer Service team is able to provide information on
whether the equipment can be used for a specific application or
modified to handle the planned process.
Experience of working with the equipment will be published semi-
regularly on the manufacturer’s website in the form of “application
notes”.
Furthermore, our colleagues are always interested in new infor-
mation and experiences resulting from user’s applications for the
equipment that may be valuable for the continued development of
our products.

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2 Safety and Responsibility
This section describes general considerations relating to user
safety that must be taken into account when working with the
equipment.
In the remaining sections, warning notices are used only to high-
light particular hazards directly arising from the actions being de-
scribed in the section in question.
It is essential to read the operating manual carefully – espe-
cially this section and the warning notices in the text – and to
follow the instructions therein.
This section also refers to areas that are the responsibility of the
provider due to certain risks arising from particular applications for
which the equipment is used deliberately and with full awareness
of the associated risks.
2.1 Intended Use, Incorrect Use and Misuse
The bench-top bioreactor Labfors 5 from INFORS HT is de-
signed especially for running bio processes with microorgan-
isms or animal cells for research and development in a bio-
technology laboratory.
The equipment is designed and constructed exclusively for the in-
tended use described above.
Intended use also includes following all the instructions in this op-
erating manual, especially those relating to:
The installation site
User qualifications
Correct operation and maintenance
The use of undamaged tubing and glass vessels
Any failure to observe the requirements specified in this manual
shall be deemed incorrect use.
Any use of the equipment outside the scope of the intended use as
described above shall be deemed misuse.
This also applies to applications for which the equipment is not de-
signed, such as the use or production of explosive gases, which is
not permitted because the equipment is not explosion-proof.

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For use for special applications not covered by conventional, in-
tended use, the equipment must be modified and certified accord-
ingly by the manufacturer.
Any use of the equipment outside of a biotechnology laboratory,
i.e. in any environment in which the conditions required for the
safety of the users cannot be fulfilled or cannot be fulfilled to their
full extent, shall also be deemed misuse.
2.2 Qualified Personnel
Due to the complexity of the equipment and the potential risks aris-
ing from its operation, the equipment may only be used by quali-
fied, specialist personnel.
2.2.1 Provider
The term “provider” applies to all persons who are responsible for
making the equipment and the necessary infrastructure available.
These persons may also be included in the group of people known
as “users”, though this is not always the case.
Irrespective of whether a provider is a member of the company’s
board of management or a supervisor, they bear a special level of
responsibility with regard to the processes and the qualification and
safety of the users.
2.2.2 User
General
The term “user” applies to all persons who come into contact with
the equipment in any way and perform work on or with it. This pri-
marily applies to the following activities, which can be performed by
the manufacturer’s own specialists or a variety of other persons (it
is not always possible to distinguish clearly between the different
types of person):
Assembly, installation and commissioning
Definition and preparation of the process
Operation
Troubleshooting and remedying of faults
Maintenance and cleaning (autoclaving, if necessary)
Service work and repairs
Disassembly, disposal and recycling

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Qualified personnel
On account of their specific education, training and – in many
cases – experience, the qualified personnel required for this work
are able to recognise risks and respond accordingly to potential
hazards.
The qualified personnel (either internal or external) who cannot be
categorised under the separate “operators” group are made up of
the following groups of persons:
Electricians (electrical engineers)
Decontamination specialists
Repair specialists
Specialists in disassembly and (environmentally friendly) dis-
posal
Recycling specialists
2.2.3 Operator
The “operators” are a specific sub-group of users distinguished by
the fact that they work with the equipment. They are the true target
audience for this operating manual.
Qualified technicians
Only technicians who have been trained for working in a biotech-
nology laboratory can be considered for the role of operator. These
include:
Process technicians in the fields of biotechnology and chemis-
try
Biotechnologists (biotechnicians)
Chemists with a specialisation in biochemistry; chemists in the
field of organic chemistry or biochemistry
Life scientists (biologists) with special education in cytology,
bacteriology, molecular biology, genetics, etc.
Lab assistants (lab technicians) from various fields
In order to be classed as a “sufficiently qualified technician” for the
operation of the equipment, the persons in question must have re-
ceived thorough training and have read and understood the operat-
ing manual.
The operator must be informed in a training session provided by
the provider of the tasks delegated to the operator and the poten-
tial risks of improper conduct. Tasks that go beyond the scope of
operation under normal conditions may only be performed by the
operator if this is specified in the manual and the provider has ex-
plicitly entrusted said tasks to the operator.

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Technicians in training
Persons in this group who are undergoing training or apprentice-
ships are only permitted to use the equipment under supervision
and in accordance with the instructions of a trained and qualified
technician.
2.3 Unauthorised Persons
The term “unauthorised persons” applies to all persons who can
access the work area but are not qualified to use the equipment in
accordance with the aforementioned requirements.
Unauthorised persons are not permitted to operate the equipment
or use it in any other way.
2.4 Responsibility of the Provider
The equipment is used for industrial and scientific purposes. As
such, the provider of the equipment is individually liable with regard
to the legal requirements relating to occupational health and safety
in a biotechnology laboratory. In particular:
The provider is responsible for ensuring that the work and en-
vironmental regulations applicable in a biotechnology labora-
tory are observed.
The provider must ensure that the equipment remains in safe
and proper working condition throughout its entire term of use.
The provider must ensure that all safety equipment is fully
functional and is not disabled.
The provider must ensure that the equipment is only worked
on by qualified users, and that said users receive sufficient
training.
The provider must ensure that the protective equipment re-
quired for working with the equipment is provided and worn.
The provider must ensure that this operating manual remains
in the immediate vicinity of the equipment throughout its entire
term of use.
2.5 General Hazards
This section covers general hazards and residual risks that are al-
ways present when using the equipment in accordance with nor-
mal, intended use.

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The following notices are general in nature. As such, with a few
exceptions they are not repeated in the remaining sections.
2.5.1 Electrical Current
The equipment runs on electrical power. There is an immediate
risk of fatal injury if contact is made with live parts.
The following points must be observed in order to avoid the risk of
fatal injury:
In case of damage to insulation, disconnect the equipment
from the mains immediately and arrange for it to be repaired.
Disconnect the equipment from the mains before commencing
any work on the electrical system.
Always use qualified electricians for any work on the electrical
system.
Keep moisture away from live parts. It may lead to a short cir-
cuit.
2.5.2 Unauthorised Spare Parts and Accessories
Incorrect or imitated spare parts and accessories as well as spare
parts or accessories that have not been authorised by the manu-
facturer represent a significant safety risk. As such, we recommend
procuring all spare parts and accessories from an authorised
dealer or directly from the manufacturer. For the contact details of
the manufacturer’s representatives, see page 2.
2.6 Particular Hazards
This section covers particular hazards and residual risks that may
arise when using the equipment for special applications in accord-
ance with normal, intended use.
Since the use of the equipment for such applications is deliberate,
it is the responsibility of the operators and the provider to ensure
that all personnel are protected from potential damage to health.
The provider is responsible for ensuring that the appropriate pro-
tective equipment for such applications is provided, and that the
necessary infrastructure is in place.

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2.6.1 Hot Surfaces
For processes that are carried out with temperatures over 55 °C,
there is a danger of burns on hot surfaces.
Since the equipment is intended for applications at high tempera-
tures, it is the responsibility of the users to ensure that they have
sufficient protection.
The motor gets hot during operation. There is a risk of burns if it is
touched.
2.6.2 Dangerous Gases
The use or production of dangerous gases i.e. toxic or asphyxiant
gases entails a significant health risk, especially in enclosed
spaces.
In order to prevent high emissions of dangerous gases, the follow-
ing measures must be taken:
The gas connections on the equipment must be checked be-
fore any cultivation processes using dangerous gases are initi-
ated.
The gaskets on the equipment must be checked at regular
intervals and replaced if necessary.
Siphon off exit gas safely.
2.6.3 Flammable or Explosive Substances
The use or production of flammable or explosive substances is not
covered under “intended use” of the equipment, as the equipment
is not explosion-proof.
If the provider intends to use the equipment for such purposes, he
must check its suitability for the planned application with the re-
sponsible local authorities.
2.6.4 Corrosive or Toxic Substances
The use or production of corrosive or toxic substances entails a
significant health risk. As such, special measures must be taken to
protect the users for such applications.
Since the equipment is used deliberately for such applications, it is
the responsibility of the users to ensure that they have sufficient
protection.

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Safety and Responsibility
22 January 2019 Page 17 of 180
2.6.5 Bioactive Substances or Pathogenic Organisms
The use or production of bioactive substances, pathogenic organ-
isms or genetically modified cultures entails a significant health
risk. As such, special measures must be taken to protect the users
for such applications.
Since the equipment is used deliberately for such applications, it is
the responsibility of the users to ensure that they have sufficient
protection.
2.6.6 Overpressure or Vacuum
Glass vessels may break or shatter when subjected to overpres-
sure or vacuums.
2.7 Warning Symbols on the Equipment
The following warning symbols (stickers) are attached to the equip-
ment:
Position
Motor
WARNING
Illegible or missing warning symbols on the equipment will lead
to the user being exposed to risks that the warning symbols in
question were designed to make him or her aware of.
It is the provider’s responsibility to ensure that all the stickers
with warning symbols on the equipment are always intact.

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Page 18 of 180 22 January 2019
2.8 Declaration of Decontamination
When returning the equipment for repair, disassembly or disposal,
it is required for the safety of all parties involved and because of
legal provisions that a lawful declaration of decontamination is pre-
sent.
The following must be observed if this is the case:
The equipment, the component part or accessory must be en-
tirely decontaminated before sending to the manufacturer
The provider is therefore required to completely and truthfully
fill out a declaration of decontamination, and have it signed by
the person responsible.
The declaration of decontamination must be affixed on
the outer packaging in which the equipment is sent back.
These forms can be obtained from the licensed dealer or the
manufacturer. See address on page 2.
Important notice
If the return shipment is not accompanied by a signed and com-
plete declaration of decontamination or it is not affixed to the outer
packaging, the shipment will be returned unopened to the sender
at their expense (see also T&C).