Thermo Scientific E-Gel 4466611 User manual

For Research Use Only. Not for use in diagnostic procedures.
E‑Gel™Imager Gel Documentation System
USER GUIDE
For visualization and documentation of gels
Catalog Numbers 4466611and 4466612
Publication Number MAN0004868
Revision A.0

Life Technologies Corporation | 5781 Van Allen Way | Carlsbad, CA 92008
For descriptions of symbols on product labels or product documents, go to thermofisher.com/symbols-definition.
The information in this guide is subject to change without notice.
DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC. AND/OR ITS AFFILIATE(S) WILL NOT BE
LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR
ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.
Revision history: Pub. No. MAN0004868
Revision Date Description
A.0 24 June 2020 Rebranded, updated, and transferred to the CCMS.
Important Licensing Information: These products may be covered by one or more Limited Use Label Licenses. By use of these
products, you accept the terms and conditions of all applicable Limited Use Label Licenses.
TRADEMARKS: All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
©2020 Thermo Fisher Scientific Inc. All rights reserved.

Contents
■CHAPTER 1 Product information .................................................. 6
Product description ............................................................. 6
Features ....................................................................... 6
Product contents ................................................................ 7
Upon receiving the device ................................................... 7
Types of E‑Gel™imager products ............................................. 7
Components ............................................................... 7
Accessory products ......................................................... 8
Description of parts ............................................................. 9
E‑Gel™Imager System ...................................................... 9
E‑Gel™Imager Camera Hood ................................................ 10
E‑Gel™Imager Base Unit ................................................... 11
Colored emission filters ..................................................... 13
■CHAPTER 2 Methods ............................................................. 14
Minimum PC requirements ...................................................... 14
Installing the E‑Gel™imager gel documentation system ............................. 14
Install hardware ................................................................ 14
Install the E‑Gel™Imager Camera Hood and Light Base ........................ 15
Using the E‑Gel™Imager gel documentation system ................................ 16
Set up the E‑Gel™Imager ................................................... 16
Launch the GelCapture Software ............................................ 18
Adjust image settings ...................................................... 18
Define region for image capture ............................................. 19
Capture and save image ................................................... 20
Maintenance .................................................................. 20
■CHAPTER 3 GelCapture software ................................................ 21
GelCapture software ........................................................... 21
Home screen .................................................................. 21
Live mode screen .............................................................. 22
Description of live mode control panel tools ....................................... 23
Edit mode screen .............................................................. 24
E
‑ Gel™Imager Gel Documentation System User Guide 3

Description of edit mode control panel tools ....................................... 25
Description of menu based tools ................................................. 26
About the histogram ............................................................ 27
Perform extended detection ..................................................... 27
Correct overexposure ........................................................... 28
Apply auto-contrast ............................................................ 28
Invert an image ................................................................ 29
Apply pseudo colors ........................................................... 29
■APPENDIX A Troubleshooting .................................................... 30
■APPENDIX B Product specifications ............................................ 32
E‑Gel™Imager Camera Hood .................................................... 32
E‑Gel™Imager Light Base (UV or Blue-Light) ...................................... 32
■APPENDIX C Software installation ............................................... 33
Getting started ................................................................ 33
Minimum PC requirements .................................................. 33
Software installation guidelines .............................................. 33
Install GelCapture Software ..................................................... 33
Windows™XP professional .................................................. 33
Windows™7 professional ................................................... 34
Installing the camera driver (Windows™7 pro) ..................................... 35
Installing the camera driver (Windows™7 professional) ......................... 35
■APPENDIX D The E‑Gel™Imager Band Excision Kit ........................... 42
Using the E‑Gel™Imager Band Excision Kit ........................................ 42
Excise a band from the gel .................................................. 44
■APPENDIX E The White Light Converter ........................................ 45
Using the White Light Converter ................................................. 45
View a gel with the white light converter ...................................... 45
■APPENDIX F Related products ................................................... 47
Accessory products ............................................................ 47
E‑Gel™agarose gels and DNA ladders ............................................ 48
Staining and detection .......................................................... 48
Contents
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‑ Gel™Imager Gel Documentation System User Guide

■APPENDIX G Explanation of symbols ........................................... 49
■APPENDIX H Products no longer supported .................................... 50
The E‑Gel™Imager iBase™Light Diuser .......................................... 50
E‑Gel™Imager Adaptor Base ................................................ 50
About multiple image acquisition ............................................ 51
Multiple image acquisition live mode screen ................................... 51
Multiple image acquisition edit screen ........................................ 52
Perform multiple image acquisition ........................................... 53
Additional multiple image acquisition functions ................................ 55
Using the E‑Gel™imager light base with E‑Gel™iBase™device ....................... 56
Hardware configuration ..................................................... 56
Using the E‑Gel™Imager Adaptor Base ........................................... 57
Install hardware ........................................................... 57
Hardware configuration ..................................................... 59
The E‑Gel™Imager iBase™Light Diuser .......................................... 61
Using the E‑Gel™Imager iBase™Light Diuser ................................ 61
■APPENDIX I Safety ................................................................ 63
Safety features ................................................................ 63
Safety requirements ............................................................ 63
Chemical safety ................................................................ 64
Biological hazard safety ......................................................... 65
■Documentation and support ....................................................... 66
Customer and technical support ................................................. 66
Limited product warranty ........................................................ 66
Contents
E ‑ Gel™Imager Gel Documentation System User Guide 5