Thermo Scientific E-Gel 4466611 User manual

For Research Use Only. Not for use in diagnostic procedures.
E‑Gel™Imager Gel Documentation System
USER GUIDE
For visualization and documentation of gels
Catalog Numbers 4466611and 4466612
Publication Number MAN0004868
Revision A.0

Life Technologies Corporation | 5781 Van Allen Way | Carlsbad, CA 92008
For descriptions of symbols on product labels or product documents, go to thermofisher.com/symbols-definition.
The information in this guide is subject to change without notice.
DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC. AND/OR ITS AFFILIATE(S) WILL NOT BE
LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR
ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.
Revision history: Pub. No. MAN0004868
Revision Date Description
A.0 24 June 2020 Rebranded, updated, and transferred to the CCMS.
Important Licensing Information: These products may be covered by one or more Limited Use Label Licenses. By use of these
products, you accept the terms and conditions of all applicable Limited Use Label Licenses.
TRADEMARKS: All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
©2020 Thermo Fisher Scientific Inc. All rights reserved.

Contents
■CHAPTER 1 Product information .................................................. 6
Product description ............................................................. 6
Features ....................................................................... 6
Product contents ................................................................ 7
Upon receiving the device ................................................... 7
Types of E‑Gel™imager products ............................................. 7
Components ............................................................... 7
Accessory products ......................................................... 8
Description of parts ............................................................. 9
E‑Gel™Imager System ...................................................... 9
E‑Gel™Imager Camera Hood ................................................ 10
E‑Gel™Imager Base Unit ................................................... 11
Colored emission filters ..................................................... 13
■CHAPTER 2 Methods ............................................................. 14
Minimum PC requirements ...................................................... 14
Installing the E‑Gel™imager gel documentation system ............................. 14
Install hardware ................................................................ 14
Install the E‑Gel™Imager Camera Hood and Light Base ........................ 15
Using the E‑Gel™Imager gel documentation system ................................ 16
Set up the E‑Gel™Imager ................................................... 16
Launch the GelCapture Software ............................................ 18
Adjust image settings ...................................................... 18
Define region for image capture ............................................. 19
Capture and save image ................................................... 20
Maintenance .................................................................. 20
■CHAPTER 3 GelCapture software ................................................ 21
GelCapture software ........................................................... 21
Home screen .................................................................. 21
Live mode screen .............................................................. 22
Description of live mode control panel tools ....................................... 23
Edit mode screen .............................................................. 24
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‑ Gel™Imager Gel Documentation System User Guide 3

Description of edit mode control panel tools ....................................... 25
Description of menu based tools ................................................. 26
About the histogram ............................................................ 27
Perform extended detection ..................................................... 27
Correct overexposure ........................................................... 28
Apply auto-contrast ............................................................ 28
Invert an image ................................................................ 29
Apply pseudo colors ........................................................... 29
■APPENDIX A Troubleshooting .................................................... 30
■APPENDIX B Product specifications ............................................ 32
E‑Gel™Imager Camera Hood .................................................... 32
E‑Gel™Imager Light Base (UV or Blue-Light) ...................................... 32
■APPENDIX C Software installation ............................................... 33
Getting started ................................................................ 33
Minimum PC requirements .................................................. 33
Software installation guidelines .............................................. 33
Install GelCapture Software ..................................................... 33
Windows™XP professional .................................................. 33
Windows™7 professional ................................................... 34
Installing the camera driver (Windows™7 pro) ..................................... 35
Installing the camera driver (Windows™7 professional) ......................... 35
■APPENDIX D The E‑Gel™Imager Band Excision Kit ........................... 42
Using the E‑Gel™Imager Band Excision Kit ........................................ 42
Excise a band from the gel .................................................. 44
■APPENDIX E The White Light Converter ........................................ 45
Using the White Light Converter ................................................. 45
View a gel with the white light converter ...................................... 45
■APPENDIX F Related products ................................................... 47
Accessory products ............................................................ 47
E‑Gel™agarose gels and DNA ladders ............................................ 48
Staining and detection .......................................................... 48
Contents
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‑ Gel™Imager Gel Documentation System User Guide

■APPENDIX G Explanation of symbols ........................................... 49
■APPENDIX H Products no longer supported .................................... 50
The E‑Gel™Imager iBase™Light Diuser .......................................... 50
E‑Gel™Imager Adaptor Base ................................................ 50
About multiple image acquisition ............................................ 51
Multiple image acquisition live mode screen ................................... 51
Multiple image acquisition edit screen ........................................ 52
Perform multiple image acquisition ........................................... 53
Additional multiple image acquisition functions ................................ 55
Using the E‑Gel™imager light base with E‑Gel™iBase™device ....................... 56
Hardware configuration ..................................................... 56
Using the E‑Gel™Imager Adaptor Base ........................................... 57
Install hardware ........................................................... 57
Hardware configuration ..................................................... 59
The E‑Gel™Imager iBase™Light Diuser .......................................... 61
Using the E‑Gel™Imager iBase™Light Diuser ................................ 61
■APPENDIX I Safety ................................................................ 63
Safety features ................................................................ 63
Safety requirements ............................................................ 63
Chemical safety ................................................................ 64
Biological hazard safety ......................................................... 65
■Documentation and support ....................................................... 66
Customer and technical support ................................................. 66
Limited product warranty ........................................................ 66
Contents
E ‑ Gel™Imager Gel Documentation System User Guide 5

Product information
Product description
The E‑Gel™Imager Gel Documentation System is a benchtop gel imaging system that
supports both fluorescent and colorimetric (visible dye) visualization applications. The
system consists of a high-resolution camera for capturing images, and a choice of
interchangeable bases to provide transillumination.
•The E‑Gel™Imager UV Light Base is used to visualize ethidium bromide stained
gels (including E‑Gel™precast agarose gels).
•The E‑Gel™Imager Blue Light Base is used to visualize gels stained with SYBR™
dyes (e.g., SYBR™Safe), including E‑Gel™precast agarose gels with SYBR Safe
and E-Gel EX precast agarose gels..
The system is controlled by the GelCapture Software application (for PC running
Windows™XP, Windows™7, or Windows™10 32-bit or 64-bit operating systems) and
allows adjustment of exposure, sensitivity, and brightness. Captured images are
exported to GelQuant Express Software for data analysis.
Features
•Small footprint to conserve benchtop space.
•Base units with blue-light transillumination avoid the risks associated with UV
transillumination.
•Sensors permit illumination only when the E‑Gel™Imager Camera Hood is
properly positioned over the E‑Gel™Imager Light Base unit.
•Extended detection feature enables viewing precise images of high and low
band intensity by expanding the dynamic range of the image.
•Compatible with a wide range of fluorescent and visible dyes (e.g. Qdot™,
SYBR™Safe, ethidium bromide).
•Compatible with dierent gel formats including precast gels (e.g. E‑Gel™Agarose
Gels, NuPAGE™Novex™Bis-Tris Gels) and pour-it-yourself gels (agarose, or
polyacrylamide).
•Rapid acquisition of high resolution images.
•Real-time sample imaging allowing detailed sample viewing.
•Eliminates the need for film or processing chemicals.
•Direct camera to PC image transfer prevents loss of resolution common to
systems employing frame-grabber cards to capture data from video signals.
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Product contents
Upon receiving the device
Examine the unit carefully for any damage incurred during transit. Any damage claims
must be filed with the carrier. The warranty does not cover in-transit damage.
Types of E‑Gel™imager products
The E‑Gel™Imager Gel Documentation System consists of an E‑Gel™Imager Camera
Hood Kit, and one of three E‑Gel™Imager Light Base Kits depending upon the
product that was purchased.
Product Catalog No.
E‑Gel™Imager with E‑Gel™Imager UV Light Base 4466611
E‑Gel™Imager with E‑Gel™Imager Blue Light Base 4466612
Components
The contents of the E‑Gel™Imager Gel Documentation System are listed in the
following table.
Component Cat. No.
4466611
Cat. No.
4466612
E‑Gel™Imager Camera Hood Kit
Camera Hood ✓ ✓
Camera Hood USB 2.0 cable ✓ ✓
E‑Gel™Imager Light Diuser ✓ ✓
E‑Gel™Imager Universal Filter ✓ ✓
GelCapture Imaging Software and GelQuant Express
Quantitation Software ✓ ✓
GelQuant Express Software Activation Dongle ✓ ✓
Quick Reference Card ✓ ✓
E‑Gel™Imager UV Light Base Kit
E‑Gel™Imager UV Light Base ✓—
Universal 12 V Power Cord ✓—
Chapter 1 Product information
Product contents 1
E ‑ Gel™Imager Gel Documentation System User Guide 7

(continued)
Component Cat. No.
4466611
Cat. No.
4466612
E‑Gel™Imager Blue Light Base Kit
E‑Gel™Imager Blue Light Base — ✓
Universal 12 V Power Cord — ✓
Accessory products
Accessory products that can be purchased for use with the E‑Gel™Imager system
are listed in the following section:
E‑Gel™imager band excision kit
Components Cat. No. Quantity
E‑Gel™Imager Band Excision Kit 4466605 1
E‑Gel™imager universal filter
Components Cat. No. Quantity
E‑Gel™Imager Universal Filter 4466606 1
E‑Gel™imager Qdot™625 filter
Components Cat. No. Quantity
E‑Gel™Imager Qdot™625 Filter 4466607 1
E‑Gel™imager UV/SYBR™filter
Components Cat. No. Quantity
E‑Gel™Imager UV/SYBR™Filter 4466608 1
GelQuant express software activation dongle
Components Cat. No. Quantity
GelQuant Express Software Activation
Dongle
4466610 1
Chapter 1 Product information
Product contents
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Description of parts
E‑Gel™Imager System
The E‑Gel™Imager is a compact gel imaging tool standing 43.7 cm in height. It
consists of the E‑Gel™Imager Camera Hood (35.6 cm in height), which fits on top of
a Base unit (11.9 cm in height). The base unit provides transillumination for
visualization of the gel.
Front view – camera hood on light base Rear view – camera hood on light base
1
3
4
1Camera hood ON/OFF power switch
2USB port
3Emission filter
4Light base ON/OFF power switch
5Light base power port
6iBase power cable port
2
3
6
5
Chapter 1 Product information
Description of parts 1
E ‑ Gel™Imager Gel Documentation System User Guide 9

E‑Gel™Imager Camera Hood
The camera hood contains the camera and emission filter. It must be connected to a
computer installed with the GelCapture Software in order to operate. Image settings
can be adjusted using the software, or manually using the dials on the camera hood.
1
2
3
5
4
1ON/OFF power switch
2Manual iris dial
3Manual focus dial
4Filter tray
5Emission filter
Chapter 1 Product information
Description of parts
1
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‑ Gel™Imager Gel Documentation System User Guide