Infors HT Multitron User manual

Multitron
Operating Manual
2020-08-20
We bring life to your laboratory.

The best Multitron
ever
Multitron – Rel. 4.0
Incubation Shaker
FW: 2.10
Doc-Nr. 77408 - EN
V.02.00 - Original
This operating manual can also be
found online at:
www.infors-ht.com/en/multitron
Infors AG
Headoffice, Switzerland
Rittergasse 27
CH-4103 Bottmingen
T [phone]
[e-mail]
[e-mail]

Multitron - Operating Manual
Table of Contents
20 August 2020 Page 1of 158
1General Information...............................................................7
1.1 About this Manual ..........................................................7
1.2 Explanation of Special Notices .........................................8
1.2.1 Warning Notices ..............................................8
1.2.2 Other Notices...................................................8
1.3 Device Identification (Standard Identification Plate) .........9
1.4 Declaration of Conformity ...............................................9
1.5 Customer Service and Services ........................................9
2Safety and Responsibility ....................................................10
2.1 Intended Use, Incorrect Use and Misuse ........................10
2.2 Cultivation Vessels to Be Used.......................................11
2.3 Qualified Personnel .......................................................13
2.3.1 Provider .........................................................13
2.3.2 User...............................................................13
2.3.3 Operator........................................................14
2.4 Unauthorised Persons....................................................15
2.5 Responsibility of the Provider.........................................15
2.6 General Hazards............................................................15
2.6.1 Electrical Current ...........................................16
2.6.2 Unauthorised Spare Parts and Accessories .....16
2.7 Particular Hazards..........................................................16
2.7.1 Danger due to Hot Surfaces...........................17
2.7.2 Dangerous Gases...........................................17
2.7.3 Flammable or Explosive Substances................17
2.7.4 Corrosive or Toxic Substances ........................17
2.7.5 Pathogenic Organisms ...................................18
2.8 Safety Features..............................................................18
2.9 Warning Symbols on the Device ....................................19
2.10 Declaration of Decontamination....................................20
3Setup and Function ..............................................................21
3.1 Setup of the Basic Unit..................................................21
3.2 Basic Functions..............................................................25
3.2.1 Standard Function Shaking ............................25
3.2.2 Standard Function Temperature Control
(Heating)........................................................28
3.3 Connections and Interfaces ...........................................29
3.3.1 Mains Connection .........................................29
3.3.2 Alarm Connection .........................................29
3.3.3 Ethernet Interface ..........................................30

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3.4 Openings ......................................................................30
3.4.1 Discharge Outlet ............................................30
3.4.2 Ventilation Opening.......................................31
3.4.3 Air Vents........................................................31
3.5 Interior Lighting.............................................................32
3.6 Frames ..........................................................................32
3.7 Operating and Indicating Elements................................33
3.7.1 Power Switch.................................................33
3.7.2 Operating Panel.............................................33
3.8 Markings on the Device.................................................34
3.8.1 Identification Plate .........................................34
3.8.2 Identification of the Throw ............................34
4Options..................................................................................35
4.1 Cooling .........................................................................35
4.1.1 Internal Cooling.............................................35
4.1.2 External Cooling ............................................37
4.1.3 Operating the Cooling Unit............................38
4.1.4 Specifications and Technical Data ..................38
4.2 Direct Steam Humidification ..........................................39
4.2.1 Setup and Function........................................39
4.2.2 Connection Conditions ..................................40
4.2.3 Operating the Direct Steam Humidification....41
4.2.4 Specifications and Technical Data ..................41
4.3 Pressure Elimination Unit for Direct Steam
Humidification...............................................................42
4.3.1 Setup and Function........................................42
4.3.2 Cleaning the Pressure Elimination Unit...........43
4.3.3 Autoclaving the Pressure Elimination Unit ......45
4.3.4 Dimensions ....................................................47
4.3.5 Specifications and Technical Data ..................47
4.4 CO2Control ..................................................................48
4.4.1 Setup and Function........................................48
4.4.2 Connection Conditions ..................................49
4.4.3 Operating the CO2Control ............................49
4.4.4 Specifications and Technical Data ..................49
4.5 UV Decontamination.....................................................50
4.5.1 Setup and Function........................................50
4.5.2 Operating the UV Decontamination ...............50
4.5.3 Specifications and Technical Data ..................50
4.6 Removable Shelf............................................................51

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4.7 LED Lighting..................................................................52
4.7.1 Setup and Function........................................52
4.7.2 Operating the LED Lighting............................52
4.7.3 Specifications and Technical Data ..................52
4.8 Darkening .....................................................................53
4.9 Analog Output ..............................................................53
4.9.1 Setup and Function........................................53
4.9.2 Connection Assignment.................................53
4.10 Cable Pass-through .......................................................54
4.10.1 Setup and Function........................................54
4.10.2 Using the Cable Pass-through ........................55
4.11 Antimicrobial Coating ...................................................56
5Accessories ............................................................................57
5.1 Trays .............................................................................57
5.1.1 Universal Table Tray .......................................57
5.1.2 Pre-Fitted Trays ..............................................58
5.1.3 Tray with Sticky Stuff.....................................63
5.2 Clamps and Other Holders ............................................65
5.2.1Clamps ..........................................................66
5.2.2 Test Tube Holders ..........................................67
5.3 Box for Microtitre Plates ................................................68
5.4 eve®..............................................................................71
6Installation ............................................................................72
6.1 Operating Conditions at the Installation Location ..........72
6.2 Requirements for the Mains Connection .......................73
6.3 Minimum Distances to the Device .................................74
7Operation ..............................................................................76
7.1 Switching on the Device................................................76
7.2 Loading the Device........................................................76
7.2.1 Opening the Door..........................................76
7.2.2 Inserting and Removing the Tray....................77
7.2.3 Fitting the Holders .........................................80
7.2.4 Tips & Tricks for Loading the Tray ..................82
7.3 Overview about the Display und Controls......................84
7.3.1 Display Area...................................................85
7.3.2 Signs and Messages Regarding the Timer
Function ........................................................86
7.3.3 Error, Warning, and Alarm Signs....................87
7.3.4 Operating Panel.............................................88

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7.4 Adjusting, Activating and Deactivating Parameters........90
7.4.1 Overview about the Parameters .....................90
7.4.2 Setting the Parameter Setpoints.....................92
7.4.3 Turning a Parameter On or Off ......................93
7.5 Timer Function ..............................................................95
7.5.1 Overview .......................................................95
7.5.2 Programming the Timer - Single Change .......99
7.5.3 Programming the Timer - Cyclic Change......104
7.5.4 Changing Timer Settings during Activated
Timer Function.............................................105
7.5.5 Changing Parameter Setpoint Values
during Activated Timer Function ..................106
7.5.6 Stopping the Timer Function........................107
7.6 Using eve®to Operate the Device................................109
7.7 Settings Menu (Option Function).................................111
7.7.1 Setting the Upper Limit for the Rotation
Speed ..........................................................111
7.7.2 Setting the Upper Limit for the
Temperature................................................112
7.7.3 Setting the Lower Limit for the
Temperature................................................112
7.7.4 Setting the Brake Force for Stopping the
Table ...........................................................112
7.7.5 Activating or Deactivating the Key Pad
Lock (with a PIN)..........................................113
7.7.6 Activating or Deactivating the Key Tone ......113
7.7.7 Setting the Interior Lighting .........................114
7.7.8 Activating or Deactivating the Timer
Function ......................................................114
7.7.9 Setting up Data Exchange via Ethernet ........115
7.7.10 Setting the Height Above Sea Level
(Altimeter) ...................................................115
7.7.11 Activating or Deactivating the Door Alarm...115
7.7.12 Humidity – Switch On/Off High Alarm .........116
7.8 Setting the Adjustable Throw ......................................116
7.9 Switching Off the Device .............................................117
7.10 Behaviour in Case of Interrupted Power Supply ...........117

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8Rectifying Faults .................................................................118
8.1 Alarm Messages..........................................................119
8.1.1 Parameter Alarms (High / Low).....................119
8.1.2 Alarm Message RESTARTED .........................119
8.2 Faults and Error Messages ...........................................120
8.2.1 Error Messages Explained.............................120
8.2.2 Fault Tables..................................................123
8.3 Replacing Fuses...........................................................127
8.4 Returning for Repair ....................................................127
9Maintenance and Cleaning................................................128
9.1 Maintenance ...............................................................128
9.2 Cleaning and Disinfection ...........................................129
9.2.1 Cleaning ......................................................129
9.2.2 Disinfection..................................................132
10 Transport and Storage .......................................................133
10.1 Transport ....................................................................133
10.2 Storage .......................................................................133
11 Disassembly and Disposal..................................................134
11.1 Disassembly.................................................................134
11.2 Disposal ......................................................................135
12 Technical Data and Specifications.....................................136
12.1 Dimension Drawings ...................................................136
12.2 Connections................................................................138
12.3 Specifications of the Basic Unit....................................140
12.3.1 Weight and Dimensions...............................140
12.3.2 Electrical Connection and Power Values.......141
12.3.3 Connections and Interfaces..........................141
12.3.4 Interior Lighting ...........................................142
12.3.5 Material.......................................................142
12.3.6 Emissions .....................................................142
12.3.7 Operating Conditions ..................................142
12.3.8 Protection Type............................................143
12.3.9 Operating and Auxiliary Materials ................143
12.4 Specifications of Standard Parameters.........................144
12.4.1 Rotation Speed Parameter (Shaker Drive) .....144
12.4.2 Temperature Parameter (Heating and
Ventilation) ..................................................145

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12.5 Specifications of the Options.......................................145
12.5.1 Internal Cooling ...........................................145
12.5.2 External Cooling ..........................................147
12.5.3 Direct Steam Humidification ........................148
12.5.4 Pressure Elimination Unit for Direct Steam
Humidification .............................................149
12.5.5 CO2 Control .................................................150
12.5.6 UV Decontamination ...................................151
12.5.7 LED Lighting ................................................151
13 EC-Declaration of Conformity ...........................................152
14 Index....................................................................................153

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General Information
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1General Information
1.1 About this Manual
This manual enables the safe and efficient handling of the device.
All the information and instructions in this operating manual comply
with the current standards, legal regulations, the latest technological
and scientific developments and the knowledge gained from the
manufacturer’s many years of experience in this field.
This operating manual is a component part of the device. It
must be kept near to the device and must be accessible to the
operators at all times.
The users must read the operating manual thoroughly and fully un-
derstand its contents before beginning any work.
Adhering to all the safety and operating instructions in this manual is
essential to ensure that work is carried out safely.
The scope of delivery may differ from the explanations, descriptions
and figures in this operating manual due to special designs, addi-
tional options specified on ordering and the latest technical/mechani-
cal modifications.
This manual contains illustrations to aid general understanding. These
may differ from the actual device as supplied.

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1.2 Explanation of Special Notices
1.2.1 Warning Notices
Warning notices in this manual are indicated by a coloured bar and
begin with a signal word that signifies the degree of the hazard.
DANGER
The signal word “DANGER” indicates a dangerous situation that
will lead to severe or even fatal injuries if not avoided.
WARNING
The signal word “WARNING” indicates a potentially dangerous sit-
uation that may result in severe or even fatal injuries if not
avoided.
CAUTION
The signal word “CAUTION” indicates a potentially dangerous sit-
uation that may result in minor injuries if not avoided.
1.2.2 Other Notices
ATTENTION
The word “ATTENTION” on a blue bar indicates a situation that
may result in significant damage to property if not avoided.
INFORMATION
Texts located below a grey bar bearing the notice “INFOR-
MATION” provide useful tips and recommendations for ensuring
efficient, fault-free operation of the device.

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1.3 Device Identification (Standard Identification Plate)
The identification plate is designed to allow clear identification of the
device. It contains the following information:
Manufacturer name
Designation = Category of device
Type = Device type (name)
S/N = Serial number
Year = Year of manufacture
Mains = Nominal voltage and frequency
Current = Current consumption
Manufacturer address
CE marking
1.4 Declaration of Conformity
The device is in compliance with the essential requirements of the
following Directives:
Machinery Directive 2006/42/EC
EMC Directive 2014/30/EU
The Declaration of Conformity according to EC Machinery Directive
2006/42/EC, annex II 1 A is attached to the operating manual (see
chapter 13 "EC-Declaration of Conformity", page 152).
1.5 Customer Service and Services
Our Customer Service is at your disposal for technical advice and spe-
cialist enquiries. For contact information, see page 2.
Due to their familiarity with the potential applications of the device,
the Customer Service team is able to provide information on whether
the device can be used for a specific application or modified to han-
dle the planned process.
Furthermore, our colleagues are always interested in new information
and experiences resulting from user’s applications for the device that
may be valuable for the continued development of our products.

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Safety and Responsibility
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2Safety and Responsibility
This section describes general considerations relating to user safety
that must be taken into account when working with the device.
In the remaining sections, warning notices are used only to highlight
particular hazards directly arising from the actions being described in
the section in question.
It is essential to read the operating manual carefully – espe-
cially this section and the warning notices in the text – and to
follow the instructions therein.
This section also refers to areas that are the responsibility of the pro-
vider due to certain risks arising from particular applications for which
the device is used deliberately and with full awareness of the associ-
ated risks.
2.1 Intended Use, Incorrect Use and Misuse
Depending on its features, the device is designed to be used
as an incubation shaker for cultivating microorganisms or cell
cultures under the following conditions:
Cultivation of non-pathogenic microorganisms or cell
cultures of risk category 1 in a biotechnology labora-
tory of biological protection level 1.
Cultivation of pathogenic microorganisms or cell cul-
tures of risk category 2 in a biotechnology laboratory
of biological protection level 2.
When using the device in protection level 2, users are respon-
sible for taking appropriate protective measures to ensure
that organisms cannot escape uncontrollably due to flask
breakage, unintentional detaching of the sterile seal or simi-
lar.
WARNING
The device is designed and constructed exclusively for the intended
use described above.
Each instance of non-conventional use is considered as misuse and
may lead to dangerous situations.

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Intended use also includes following all the instructions in this man-
ual, especially those relating to:
The installation site
Use of suitable cultivation vessels
User qualifications
Permissible parameter setpoints
Correct operation and maintenance
Any failure to observe the requirements specified in this manual shall
be deemed incorrect use, in particular, use of inappropriate cultiva-
tion vessels and/or unsuitable holders at speeds that are too high.
Any use of the device outside the scope of the intended use as de-
scribed above shall be deemed misuse. This also applies to applica-
tions for which the device is not designed, especially the following:
The device is not protected against explosions. Use and manu-
facture of explosive gases as well as operating the device in the
Ex area are therefore not permitted.
The device is not designed to sufficiently protect its users if path-
ogenic organisms escape uncontrollably. Cultivation of patho-
genic organisms of risk categories 3 and 4 is therefore not per-
mitted.
To use the device for special applications not covered by conven-
tional, intended use, the device must be modified and certified ac-
cordingly by the manufacturer.
Any use of the device outside of a biotechnology laboratory, i.e. in
any environment in which the conditions required for the safety of
users cannot be met or cannot be met to their full extent, shall also
be deemed misuse.
2.2 Cultivation Vessels to Be Used
Significant forces are applied to cultivation vessels, in particular in
case of large vessels and high speeds. Hence, the cultivation vessels
used are particularly significant in relation to user safety.
ATTENTION
Use of unsuitable or defective cultivation vessels can lead to glass
breakage and therefore damage to property.

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Approved cultivation vessels
The device has been designed for use with the following vessels using
the holders designed specifically for them:
Erlenmeyer flasks up to 5,000 mL made of borosilicate glass
(e.g. Schott Duran®) or high-grade plastic, such as polycarbonate
(z. B. Corning®) etc.
Fernbach flasks up to 3,000 mL made of borosilicate glass (e.g.
Schott Duran®) or high-grade plastic, such as polycarbonate
(z. B. Corning®) etc.
Other vessels with the holders designed for them:
Test tubes
Centrifuge tubes
Microtitre plates
Deep well plates
To avoid the vessels coming out of the clamps at very high speeds,
they might have to be secured using cable ties underneath the
springs or some other suitable measure.
Cultivating organisms of risk category 2
When cultivating pathogenic organisms of risk category 2, special
measures must be taken to stop the organisms from escaping. The
user is responsible for this.
When using the device under protection category 2, stainless steel
clamps of the correct size must be used to affix the flasks. Due to lim-
ited resistance to disinfectants as well as the risk of unintentional de-
taching of flasks, Sticky Stuff adhesive matting is not suitable for this
purpose.
We further recommend using disposable plastic flasks with screw
tops and filter membranes. We recommend using sticky tape to se-
cure the lid against loosening unintentionally. Using glass flasks with
cotton wool or paper plugs is not sufficiently safe.
Trays with Sticky Stuff
INFORMATION
For trays with Sticky Stuff, special provisions apply in relation to
maximum permitted speeds. These must be observed to prevent
cultivation vessels from detaching.
For detailed information see chapter 5.1.3 "Tray with Sticky
Stuff", page 63.

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2.3 Qualified Personnel
Due to the complexity of the device and the potential risks arising
from its operation, the device may only be used by qualified, special-
ist personnel.
2.3.1 Provider
The term “provider” applies to all persons who are responsible for
making the device and the necessary infrastructure available. These
persons may also be included in the group of people known as “us-
ers”, though this is not always the case.
Irrespective of whether a provider is a member of the company’s
board of management or a supervisor, they bear a special level of
responsibility with regard to the processes and the qualification and
safety of the users.
2.3.2 User
General
The term “user” applies to all persons who come into contact with
the device in any way and perform work on or with it. This primarily
applies to the following activities, which can be performed by the
manufacturer’s own specialists or a variety of other persons (it is not
always possible to distinguish clearly between the different types of
person):
Assembly, installation and commissioning
Definition and preparation of the process
Operation
Troubleshooting and remedying of faults
Maintenance and cleaning (autoclaving, if necessary)
Service work and repairs
Disassembly, disposal and recycling
Qualified personnel
On account of their specific education, training and – in many cases –
experience, the qualified personnel required for this work are able to
recognise risks and respond accordingly to potential hazards.
The qualified personnel (either internal or external) who cannot be
categorised under the separate “operators” group are made up of
the following groups of persons:

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Electricians (electrical engineers)
Decontamination specialists
Repair specialists
Specialists in disassembly and (environmentally friendly) disposal
Recycling specialists
2.3.3 Operator
The “operators” are a specific sub-group of users distinguished by
the fact that they work with the device. They are the true target audi-
ence for this operating manual.
Qualified technicians
Only technicians who have been trained for working in a biotechnol-
ogy laboratory can be considered for the role of operator. These in-
clude:
Process technicians in the fields of biotechnology and chemistry
Biotechnologists (biotechnicians)
Chemists with a specialisation in biochemistry; chemists in the
field of organic chemistry or biochemistry
Life scientists (biologists) with special education in cytology, bac-
teriology, molecular biology, genetics, etc.
Lab assistants (lab technicians) from various fields
In order to be classed as a “sufficiently qualified technician” for the
operation of the device, the persons in question must have received
thorough training and have read and understood the operating man-
ual.
The operator must be informed in a training session provided by the
provider of the tasks delegated to the operator and the potential risks
of improper conduct. Tasks that go beyond the scope of operation
under normal conditions may only be performed by the operator if
this is specified in the manual and the provider has explicitly en-
trusted said tasks to the operator.
Technicians in training
Persons in this group who are undergoing training or apprenticeships
are only permitted to use the device under supervision and in accord-
ance with the instructions of a trained and qualified technician.

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2.4 Unauthorised Persons
The term “unauthorised persons” applies to all persons who can ac-
cess the work area but are not qualified to use the device in accord-
ance with the aforementioned requirements.
Unauthorised persons are not permitted to operate the device or use
it in any other way.
2.5 Responsibility of the Provider
The device is used for industrial and scientific purposes. As such, the
provider of the device is individually liable with regard to the legal
requirements relating to occupational health and safety in a biotech-
nology laboratory. In particular:
The provider is responsible for ensuring that the work and envi-
ronmental regulations applicable in a biotechnology laboratory
are observed.
The provider must ensure that the device remains in safe and
proper working condition throughout its entire term of use.
The provider must ensure that all safety equipment is fully func-
tional and is not disabled.
The provider must ensure that the device is only worked on by
qualified users, and that said users receive sufficient training.
The provider must ensure that the protective equipment re-
quired for working with the device is provided and worn.
The provider must ensure that this operating manual remains in
the immediate vicinity of the device throughout its entire term
of use.
2.6 General Hazards
This section covers general hazards and residual risks that are always
present when using the device in accordance with normal, intended
use.
The following notices are general in nature. As such, with a few ex-
ceptions they are not repeated in the remaining sections.

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2.6.1 Electrical Current
The device is operated electronically. There is an immediate risk of fa-
tal injury if contact is made with live parts. The following points must
be observed in order to avoid the risk of fatal injury:
In case of damage to insulation, disconnect the device from the
mains immediately and arrange for it to be repaired.
Disconnect the device from the mains before commencing any
work on the electrical system.
Always use qualified electricians for any work on the electrical
system.
Disconnect the device from the mains before beginning any
maintenance, cleaning or repair work.
Do not bypass any fuses or take them out of operation.
When replacing fuses, ensure they have the correct number of
Amperes.
If the power cable is defective, replace it with a power cable of
the same type.
Keep moisture away from live parts. It may lead to a short cir-
cuit.
Never remove covers from the casing.
2.6.2 Unauthorised Spare Parts and Accessories
Incorrect or imitated spare parts and accessories as well as spare parts
or accessories that have not been authorised by the manufacturer
represent a significant safety risk. As such, we recommend procuring
all spare parts and accessories from an authorised dealer or directly
from the manufacturer. For the contact details of the manufacturer’s
representatives, see page 2.
2.7 Particular Hazards
This section covers particular hazards and residual risks that may arise
when using the device for special applications in accordance with
normal, intended use.
Since the use of the device for such applications is deliberate, it is the
responsibility of the operators and the provider to ensure that all per-
sonnel are protected from potential damage to health. The provider
is responsible for ensuring that the appropriate protective equipment
for such applications is provided, and that the necessary infrastruc-
ture is in place.

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2.7.1 Danger due to Hot Surfaces
For applications that are performed with temperatures over 55 °C,
there is a danger of burns on hot surfaces in the interior or on the
cultivation vessels.
For applications with temperatures over 55 °C wear heat-re-
sistant protective gloves.
2.7.2 Dangerous Gases
The use or production of dangerous – i.e. toxic or asphyxiant – gases
entails a significant health risk, especially in enclosed spaces. To pre-
vent high emissions of dangerous gases, the following measures
must be taken:
When using CO2control or manufacturing dangerous gases, the
device must be set up in a well-ventilated area.
The gas connections on the device must be checked before any
cultivation processes using dangerous gases are initiated.
2.7.3 Flammable or Explosive Substances
The use or production of flammable or explosive substances is not
covered under “intended use” of the device, as the device is not ex-
plosion-proof.
If the provider intends to use the device for such purposes, he must
check its suitability for the planned application with the responsible
local authorities.
2.7.4 Corrosive or Toxic Substances
The use or production of corrosive or toxic substances entails a signif-
icant health risk. As such, special measures must be taken to protect
the users for such applications.
Since the device is used deliberately for such applications, it is the
responsibility of the users to ensure that they have sufficient protec-
tion.

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2.7.5 Pathogenic Organisms
The device is not approved for cultivation of pathogenic organisms of
risk categories 3 and 4. In the context of intended use, it is nonethe-
less possible for pathogenic organisms and viruses to be cultivated.
Contact with pathogenic organisms bears a significant health risk.
Hence, users are responsible for ensuring adequate protection.
2.8 Safety Features
The device is equipped with the following safety features:
Fuses / thermal protection switches
Two fuses (230 V version) or two thermal protection switches (115 V
version) protect the device from impermissibly high power input. The
fuses are located right next to the mains connection on the left-hand
side of the casing. For descriptions of which fuses to use for which
device type, see chapter 12.3 "Specifications of the Basic Unit
", page
140.
Overheating shut-down
The heating of the device is protected against overheating by a tem-
perature
limiter. This triggers as soon as the temperature on the heat-
ing element exceeds the maximum allowed value and immediately
turns off the heating.
Door monitoring
The position of the door is monitored electronically. If the door is
opened, the shaker drive is stopped immediately. As soon as the door
is fully closed again, the shaker drive restarts automatically.