Infors HT Multifors 2 User manual

Operating Manual
2018-03-28
Multifors 2
Parallel Bench-top Bioreactor
Dok-ID: V. 04.01 - Original

Page 2 of 211 28 March 2018
Infors AG
Headoffice, Schweiz
Rittergasse 27
CH-4103 Bottmingen
T [phone]
F [phone]
[e-mail]
[e-mail]
Infors GmbH
Dachauer Str. 6
D-85254 Einsbach
T [phone]
F [phone]
[e-mail]
Infors UK Ltd
The Courtyard Business Centre
Dovers Farm, Lonesome Lane,
Reigate
Surrey, RH2 7QT, UK
T [phone]
F [phone]
[e-mail]
Infors Sarl
2, rue du Buisson aux Fraises
Bâtiment D13
F-91300 Massy
T [phone]
F [phone]
[e-mail]
www.infors-ht.fr
Infors Benelux BV
Markweg 9-A, NL-6883 JL
Velp (GLD)
P.O. Box 125, NL-6880 AC
Velp (GLD)
T [phone]
F [phone]
[e-mail]
Infors USA Inc.
9070 Junction Drive, Suite D
Annapolis Junction, MD20701
T [phone] /
T [phone] (toll-free USA)
F [phone]
[e-mail]
www.infors-ht.us
Infors Canada
8350 rue Bombardier
Anjou, Quebec
Canada H1J 1A6
T [phone]
F [phone]
[e-mail]
Infors Bio-Technology
(Beijing) Co., Ltd.
Room 505C, Building 106
Lize Zhongyuan
Wangjing New Industrial Zone
Chaoyang District, Beijing
100102 P.R. of China
T [phone]
F [phone]
[e-mail]
Infors South East Asia
16, 1st Floor, Taman City
MY-51200 Kuala Lumpur
Malaysia
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F [phone]
[e-mail]
Infors LATAM Ltda.
Rua Dr. Alceu de Campos
Conjunto 205
CEP: 04544-000
São Paulo – SP
Brasil
T [phone]
F [phone]
[e-mail]
Contact details of our local dealers worldwide can be found
on our website.
www.infors-ht.com
Engineering and production in Switzerland

Multifors 2 - Parallel Bench-top Bioreactor
Table of Contents
28 March 2018 Page 3 of 211
1General Information ............................................................... 8
1.1 About this Manual .......................................................... 8
1.2 Symbols ......................................................................... 9
1.2.1 Safety Instructions .......................................... 9
1.2.2 Hints and Recommendations ......................... 9
1.2.3 Specific Safety Instructions .......................... 10
1.3 Limitation of Liability .................................................... 11
1.4 Copyright Protection .................................................... 11
1.5 Spare Parts .................................................................. 12
1.6 Terms of Guarantee ..................................................... 12
1.7 Customer Service ........................................................ 12
1.8 Declaration of Conformity ............................................ 12
2Safety .................................................................................... 13
2.1 Responsibility of the Operator ..................................... 13
2.2 Requirements for Qualified Personnel ......................... 14
2.2.1 Unauthorised Persons .................................. 15
2.3 Conventional Use of the Equipment ............................ 16
2.4 Personal Protective Equipment ................................... 17
2.5 Particular Hazards ....................................................... 19
2.6 Environmental Protection ............................................. 22
2.7 Adhesive Labels / Signs / Symbols ............................. 22
3Technical Data ...................................................................... 24
3.1 Equipment Dimensions ................................................ 24
3.2 Dimensions Culture Vessels and Vessel Holder ......... 25
3.3 Weights ........................................................................ 27
3.4 Service Requirements ................................................. 28
3.4.1 Electrical ....................................................... 28
3.4.2 Water IN ....................................................... 28
3.4.3 Water OUT ................................................... 28
3.4.4 Gas(es) IN .................................................... 28
3.4.5 Exit Gas ........................................................ 29
3.5 Specifications ............................................................... 30
3.5.1 Operating Unit .............................................. 30
3.5.2 Vessels ......................................................... 30
3.5.3 Stirrer Drive System bacterial Version ......... 32
3.5.4 Stirrer Drive System Cell Culture Version .... 33
3.5.5 Temperature ................................................. 35
3.5.6 Gassing Bacterial System ............................ 36
3.5.7 Gassing Cell Culture System ....................... 37
3.5.8 Antifoam ....................................................... 38
3.5.9 pH ................................................................. 38

Multifors 2 - Parallel Bench-top Bioreactor
Table of Contents
Page 4 of 211 28 March 2018
3.5.10 pO2 ................................................................39
3.5.11 Pumps ...........................................................40
3.5.12 Sterile Filters .................................................41
3.6 Operating Conditions ...................................................42
3.7 Emissions .....................................................................42
3.8 Identification Plate ........................................................43
4Setup and Function ..............................................................44
4.1 Basic Unit .....................................................................44
4.1.1 Main Switch ..................................................46
4.1.2 Mains Connection .........................................46
4.1.3 Water Connections .......................................47
4.1.4 Gas Connections ..........................................47
4.1.5 Connections of the Exit Gas Cooler and
Control Valve ................................................47
4.1.6 Connection of the Sparger ............................49
4.1.7 Pumps ...........................................................50
4.2 Operating Panel ...........................................................52
4.2.1 Monitor Keys .................................................52
4.2.2 Operating Panel Connections .......................53
4.3 Vessels, Vessel holder & Transport Frame .................54
4.3.1 Vessel for bacterial Cultures .........................55
4.3.2 Vessel for Cell Cultures ................................57
4.3.3 Baffle Cage ...................................................58
4.3.4 Vessel Top Plate Ports and Configuration ...60
4.3.4.1 Top Plate with nominal Width
55/70 ............................................ 64
4.3.4.2 Vessel Top Plate with nominal
Width 70 ....................................... 65
4.3.4.3 Vessel Top Plate with nominal
Width 90 ....................................... 66
4.4 Temperature Control System .......................................67
4.5 Drive and Stirrer ...........................................................68
4.5.1 Drive Shaft for bacterial Cultures ..................68
4.5.2 Drive Shaft for Cell Cultures .........................69
4.5.3 Impellers .......................................................70
4.6 Gassing System ...........................................................71
4.6.1 Gassing Strategy ..........................................72
4.6.2 Gas Entry ......................................................72
4.6.3 Exit Gas and Exit Gas Cooler .......................76
4.7 Accessory Kit ...............................................................77
4.7.1 Antifoam Sensor ...........................................77
4.7.2 Reagent Bottles ............................................80

Multifors 2 - Parallel Bench-top Bioreactor
Table of Contents
28 March 2018 Page 5 of 211
4.7.3 Sampling System ......................................... 81
4.7.3.1 Super Safe Sampler, aseptic
Sampling System .......................... 81
4.7.4 Starter-Kit ..................................................... 86
4.8 Field of Application ...................................................... 87
4.9 Types ........................................................................... 87
5Transport, Packaging and Storage .................................... 88
5.1 Safety Instructions for Transport .................................. 88
5.2 Transport Inspection .................................................... 88
5.3 Packaging .................................................................... 89
5.4 Transport ...................................................................... 90
5.5 Storage ........................................................................ 91
5.5.1 Storage of the Equipment in General ........... 91
5.5.2 Storage of the Reagent Bottles and Hoses .. 92
5.5.3 Storage of Sensors ....................................... 92
6Installation and Initial Operation ........................................ 93
6.1 Safety ........................................................................... 93
6.2 Location Requirements for Installation ........................ 94
6.2.1 General Information ...................................... 94
6.2.2 Minimum Spacing ......................................... 95
6.2.3 Supply Lines ................................................. 95
6.2.3.1 Air/Gas .......................................... 95
6.2.3.2 Exit Gas ......................................... 96
6.2.3.3 Water Inlet ..................................... 97
6.2.3.4 Water outlet ................................... 97
6.2.3.5 Electric Power Supply ................... 98
6.3 Mounting the Operating Panel ..................................... 99
6.4 Connecting the Operating panel to the Basic Unit..... 100
6.5 Connecting the Master Unit with the Satellite Unit(s) 101
6.5.1 Mounting the Water Connection Set for 1 or 2
Satellite Units ............................................. 102
6.5.2 Connecting the Controller of the Master Unit
with the Controllers of the Satellite Unit(s) . 104
6.6 Test Run .................................................................... 106
7Preparations for Use .......................................................... 110
7.1 Safety ......................................................................... 110
7.2 Preparations before Sterilisation................................ 112
7.2.1 Preparing the Vessel .................................. 112
7.2.2 Preparing the Sensors ................................ 121
7.2.2.1 Calibrating the pH Sensor ........... 121