GE Amersham Imager 680 User manual

Amersham™Imager 680
Operating Instructions
Original instructions
Translation disc enclosed

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Table of Contents
71 Introduction ..........................................................................................................
81.1 About this manual ................................................................................................................................
91.2 Important user information .............................................................................................................
111.3 Regulatory information ......................................................................................................................
121.3.1 EU Directives ....................................................................................................................................
131.3.2 Eurasian Customs Union ............................................................................................................
141.3.3 Regulations for USA and Canada ...........................................................................................
151.3.4 Notification - products containing mercury (Canada) ...................................................
171.3.5 Other regulations and standards ............................................................................................
192 Safety instructions ...............................................................................................
202.1 Safety precautions ...............................................................................................................................
302.2 Labels .........................................................................................................................................................
342.3 Emergency procedure ........................................................................................................................
352.4 Recycling information .........................................................................................................................
372.5 Declaration of Hazardous Substances (DoHS) ........................................................................
393 Instrument description .......................................................................................
403.1 Introduction .............................................................................................................................................
413.2 Feature overview ..................................................................................................................................
423.3 Illustration of the instrument ...........................................................................................................
433.4 Instrument hardware description .................................................................................................
503.5 Instrument software description ...................................................................................................
513.5.1 Description of the Capture tab .................................................................................................
563.5.2 Description of the Library tab ...................................................................................................
583.5.3 Description of the image view ..................................................................................................
623.5.4 Description of the analysis workflow ....................................................................................
663.5.5 Description of settings views ....................................................................................................
703.6 Accessories ..............................................................................................................................................
734 Installation ............................................................................................................
744.1 Site requirements ..................................................................................................................................
774.2 Instrument setup ..................................................................................................................................
784.3 System settings .....................................................................................................................................
794.3.1 Date and time ..................................................................................................................................
814.3.2 Focus ...................................................................................................................................................
834.3.3 Instrument indicator .....................................................................................................................
844.3.4 Service ................................................................................................................................................
864.3.5 Log in ...................................................................................................................................................
894.4 Network .....................................................................................................................................................
904.4.1 Network setup .................................................................................................................................
944.4.2 Network access from instrument ............................................................................................
964.4.3 Instrument access from network ............................................................................................
Amersham Imager 680 Operating Instructions 29271308 AB 3
Table of Contents

995 Operation ..............................................................................................................
1005.1 Operation flow chart ...........................................................................................................................
1015.2 Turn on the instrument ......................................................................................................................
1055.3 Select tray ................................................................................................................................................
1065.4 Place the tray in the instrument ....................................................................................................
1095.5 Capture an image .................................................................................................................................
1105.5.1 Available methods .........................................................................................................................
1115.5.2 Chemiluminescence capture .....................................................................................................
1375.5.3 Colorimetric capture .....................................................................................................................
1415.5.4 Fluorescence capture ...................................................................................................................
1635.6 View the image ......................................................................................................................................
1675.7 Save and manage image files ........................................................................................................
1685.7.1 Save locations .................................................................................................................................
1725.7.2 Save images .....................................................................................................................................
1785.7.3 File manage options .....................................................................................................................
1815.8 Analyze the image ................................................................................................................................
1845.8.1 Lane creation ...................................................................................................................................
1865.8.2 Background Subtraction .............................................................................................................
1905.8.3 Band detection ................................................................................................................................
1925.8.4 Molecular weight assignment ..................................................................................................
1955.8.5 Normalization ..................................................................................................................................
1965.8.6 Summary ...........................................................................................................................................
1995.9 Turn off the instrument ......................................................................................................................
2006 Analysis software .................................................................................................
2016.1 Software installation and activation ............................................................................................
2026.1.1 Amersham Imager 680 Analysis Software installation .................................................
2056.1.2 Amersham Imager 680 Analysis Software registration ................................................
2106.2 Software operation ..............................................................................................................................
2146.2.1 Lane creation ...................................................................................................................................
2166.2.2 Background subtraction .............................................................................................................
2216.2.3 Band detection ................................................................................................................................
2236.2.4 Molecular weight assignment ..................................................................................................
2266.2.5 Normalization ..................................................................................................................................
2276.2.6 Summary ...........................................................................................................................................
2307 Maintenance .........................................................................................................
2317.1 Instrument ...............................................................................................................................................
2327.2 Accessories ..............................................................................................................................................
2347.3 Regular inspections .............................................................................................................................
2358 Troubleshooting ...................................................................................................
2368.1 General problems .................................................................................................................................
2388.2 Problems with image quality ...........................................................................................................
2408.3 Problems with the software .............................................................................................................
2439 Reference information ........................................................................................
2449.1 Specifications .........................................................................................................................................
4 Amersham Imager 680 Operating Instructions 29271308 AB
Table of Contents

2479.2 Health and Safety Declaration Form ...........................................................................................
249Index .......................................................................................................................
Amersham Imager 680 Operating Instructions 29271308 AB 5
Table of Contents

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1 Introduction
About this chapter
This chapter contains important user information, description of safety notices, regula-
tory information, and a general description of the intended use of Amersham Imager
680.
In this chapter
See pageSection
81.1 About this manual
91.2 Important user information
111.3 Regulatory information
Amersham Imager 680 Operating Instructions 29271308 AB 7
1 Introduction

1.1 About this manual
Purpose of this manual
The Operating Instructions provide you with the instructions needed to operate, and
maintain Amersham Imager 680 in a safe way.
Typographical conventions
Software items are identified in the text by bold italic text. A colon separates menu levels,
thus File:Open refers to the Open command in the File menu.
Hardware items are identified in the text by bold text (for example, Power).
8 Amersham Imager 680 Operating Instructions 29271308 AB
1 Introduction
1.1 About this manual

1.2 Important user information
Read this before operating
Amersham Imager 680
All users must read the entire Operating Instructions before installing, operating or
maintaining Amersham Imager 680.
Always keep the Operating Instructions at hand when operating Amersham Imager 680.
Do not operate Amersham Imager 680 in any other way than described in the user
documentation. If you do, you may be exposed to hazards that can lead to personal injury
and you may cause damage to the equipment.
Intended use
Amersham Imager 680 is a CCD camera system that produces digitial images of samples
in gels or membranes in three different modes: chemiluminescence, colorimetric, and
fluorescence.
Amersham Imager 680 is intended for research use only, and shall not be used in clinical
procedures, or for diagnostic purposes.
Prerequisites
In order to operate Amersham Imager 680 in the intended way, the following prerequisites
must be fulfilled:
•You have read and understood the safety instructions in this Operating Instruction
•You should be acquainted with the use of general laboratory equipment and with
handling of biological materials
•The instrument is installed by GE representatives
Amersham Imager 680 Operating Instructions 29271308 AB 9
1 Introduction
1.2 Important user information

Safety notices
This user documentation contains safety notices (WARNING, CAUTION, and NOTICE)
concerning the safe use of the product. See definitions below.
WARNING
WARNING indicates a hazardous situation which, if not avoided,
could result in death or serious injury. It is important not to proceed
until all stated conditions are met and clearly understood.
CAUTION
CAUTION indicates a hazardous situation which, if not avoided,
could result in minor or moderate injury. It is important not to pro-
ceed until all stated conditions are met and clearly understood.
NOTICE
NOTICE indicates instructions that must be followed to avoid
damage to the product or other equipment.
Notes and tips
A note is used to indicate information that is important for trouble-free and
optimal use of the product.
Note:
A tip contains useful information that can improve or optimize your procedures.
Tip:
10 Amersham Imager 680 Operating Instructions 29271308 AB
1 Introduction
1.2 Important user information

1.3 Regulatory information
Introduction
This section describes the directives and standards fulfilled by Amersham Imager 680.
Manufacturing information
The table below summarizes the required manufacturing information.
InformationRequirement
GE Healthcare Bio-Sciences AB,Name and address of manufacturer
Björkgatan 30, SE 751 84 Uppsala, Sweden
In this section
See pageSection
121.3.1 EU Directives
131.3.2 Eurasian Customs Union
141.3.3 Regulations for USA and Canada
151.3.4 Notification - products containing mercury (Canada)
171.3.5 Other regulations and standards
Amersham Imager 680 Operating Instructions 29271308 AB 11
1 Introduction
1.3 Regulatory information

1.3.1 EU Directives
Conformity with EU Directives
This product fulfills the European Directives listed below. See the EU Declaration of
Conformity for the directives and regulations that apply for the CE marking.
If not included with the product, a copy of the EU Declaration of Conformity is available
on request.
TitleDirective
Machinery Directive (MD)2006/42/EC
Electromagnetic Compatibility (EMC) Directive2014/30/EU
Low Voltage Directive (LVD)2014/35/EU
Restriction of Hazardous Substances (RoHS) Directive2011/65/EU
CE marking
The CE marking and the corresponding EU Declaration of Conformity is valid for the in-
strument when it is:
•used according to the Operating Instructions or user manuals, and
•used in the same state as it was delivered from GE, except for alterations described
in the Operating Instructions or user manuals.
12 Amersham Imager 680 Operating Instructions 29271308 AB
1 Introduction
1.3 Regulatory information
1.3.1 EU Directives

1.3.2 Eurasian Customs Union
Introduction
This section contains additional regulatory information to comply with the Eurasian
Customs Union technical regulations.
Manufacturer and importer
information
The table below summarizes the manufacturer and importer information required by
the Eurasian Customs Union.
InformationRequirement
See Manufacturing informationName and address of manufacturer
Telephone: + 46 771 400 600Telephone number of manufacturer
GE Healthcare LLCImporter and/or company for obtain-
ing information about importer GE Healthcare Life Sciences
Presnenskaya nab., 10C, 12th floor
RU-123 317 Moscow, Russian Federation
Telephone 1: + 7 495 411 9714
Fax nr: + 7 495 739 6932
Email: [email protected]
Amersham Imager 680 Operating Instructions 29271308 AB 13
1 Introduction
1.3 Regulatory information
1.3.2 Eurasian Customs Union

1.3.3 Regulations for USA and Canada
NRTL certification
This symbol indicates that Amersham Imager 680 has been certified by a Nationally
Recognized Testing Laboratory (NRTL).
NRTL means an organization, which is recognized by the US Occupational Safety and
Health Administration (OSHA) as meeting the legal requirements of Title 29 of the Code
of Federal Regulations (29 CFR), Part 1910.7.
FCC compliance
This device complies with part 15 of the FCC Rules. Operation is subject to the following
two conditions: (1) This device may not cause harmful interference, and (2) this device
must accept any interference received, including interference that may cause undesired
operation.
The user is cautioned that any changes or modifications not expressly approved
by GE could void the user’s authority to operate the equipment.
Note:
This equipment has been tested and found to comply with the limits for a Class A digital
device, pursuant to part 15 of the FCC Rules. These limits are designed to provide rea-
sonable protection against harmful interference when the equipment is operated in a
commercial environment. This equipment generates, uses, and can radiate radio frequen-
cy energy and, if not installed and used in accordance with the instruction manual, may
cause harmful interference to radio communications. Operation of this equipment in a
residential area is likely to cause harmful interference in which case the user will be re-
quired to correct the interference at his own expense.
CAN ICES/NMB compliance
This product complies with the Canadian standard ICES-003/NMB-003 concerning
electromagnetic compatibility.
14 Amersham Imager 680 Operating Instructions 29271308 AB
1 Introduction
1.3 Regulatory information
1.3.3 Regulations for USA and Canada

1.3.4 Notification - products containing mercury (Canada)
Introduction
This notice is provided in accordance with the Products Containing Mercury Regulation
SOR/2014-254 November 7, 2014 as annexed in the Canadian Environmental Protection
Act, 1999.
Cette mise en garde est fournie conformément au règlement DORS/2014-254 du 7
novembre 2014 sur les produits contenant du mercure, tel qu’annexé à la loi canadienne
sur la protection de l’environnement (1999).
Notification (English)
Notification about this product.
Hg
•Contains mercury.
•For safe handling procedures and the measures to be taken in case of accidental
breakage, and for options available for disposal and recycling, please refer to
https://www.ec.gc.ca/mercuremercury/Default.asp?lang=En&n=DB6D2996-1.
If additional support is needed, please contact your GE representative.
•This product should be disposed of or recycled in accordance with the applicable
laws.
Amersham Imager 680 Operating Instructions 29271308 AB 15
1 Introduction
1.3 Regulatory information
1.3.4 Notification - products containing mercury (Canada)

Notification (français)
Notification sur ce produit.
Hg
•Contient du mercure.
•Pour les procédures de manipulation sécuritaires et les mesures à prendre en
cas de rupture accidentelle, et pour les options disponibles pour l'élimination et
le recyclage, veuillez consulter le site
https://www.ec.gc.ca/mercure-mercury/Default.asp?lang=Fr&n=DB6D2996-1.
Pour obtenir une aide supplémentaire, veuillez communiquer avec votre
représentant GE.
•Ce produit doit être éliminé ou recyclé en conformité avec les lois applicables.
16 Amersham Imager 680 Operating Instructions 29271308 AB
1 Introduction
1.3 Regulatory information
1.3.4 Notification - products containing mercury (Canada)

1.3.5 Other regulations and standards
Environmental Conformity
TitleRequirement
Waste Electrical and Electronic Equipment (WEEE)
Directive
2012/19/EU
Management Methods for the Restriction of the
Use of Hazardous Substances in Electrical and
Electronic Products.
China RoHS
Standards, machinery and
electrical equipment
Standard requirements fulfilled by this product are summarized in the table below.
DescriptionStandard
Safety of machinery. General principles for design. Risk
assessment and risk reduction.
EN ISO 12100
Safety requirements for electrical equipment for mea-
surement, control, and laboratory use - Part 1: General
requirements.
EN 61010-1, IEC 61010-1,
UL 61010-1, CAN/CSA-
C22.2 No. 61010-1
Particular requirements for laboratory equipment for
the heating of materials
IEC/EN 61010-2-010
Particular requirements for automatic and semi-auto-
matic laboratory equipment for analysis and other pur-
poses
IEC/EN 61010-2-081
Degrees of protection provided by enclosuresEN 60529
Electrical Equipment for Measurement, Control, and
Laboratory Use-EMC requirements-Part 1: General re-
quirements
IEC/EN 61326-1
(Emission according to CISPR 11, Group 1, class A)
Amersham Imager 680 Operating Instructions 29271308 AB 17
1 Introduction
1.3 Regulatory information
1.3.5 Other regulations and standards

Light sources
The safety of the LED light sources in this instrument has been assessed by risk analysis
in accordance with EN 61010-1.
Amersham Imager 680, depending on configuration, is equipped with some or all light
sources in the table below:
WavelengthLight sources
460 nmBlue LED
520 nmGreen LED
630 nmRed LED
470 nm to 635 nmWhite LED
312 nmTrans-UV-illuminator
18 Amersham Imager 680 Operating Instructions 29271308 AB
1 Introduction
1.3 Regulatory information
1.3.5 Other regulations and standards

2 Safety instructions
About this chapter
This chapter describes safety precautions, safety labels, and emergency procedures for
the Amersham Imager 680 instrument, in addition to information on the safe disposal
of the instrument.
Important
WARNING
Before installing, operating or maintaining the product, all users
must read and understand the entire contents of this chapter
to become aware of the hazards involved.
In this chapter
See pageSection
202.1 Safety precautions
302.2 Labels
342.3 Emergency procedure
352.4 Recycling information
372.5 Declaration of Hazardous Substances (DoHS)
Amersham Imager 680 Operating Instructions 29271308 AB 19
2 Safety instructions

2.1 Safety precautions
Introduction
Amersham Imager 680 is powered by mains voltage, may be equipped with a UV light
source, and is used to capture images of samples that may be hazardous. Before in-
stalling, operating or maintaining the system, you must be aware of the hazards described
in this manual.
Follow the instructions provided to avoid personal injuries, damage to the product,
or to other personnel and equipment in the area.
The safety precautions in this section are grouped into the following categories:
•General precautions
•Personal protection
•Installation
•Operation
•Maintenance
General precautions
WARNING
Do not operate the product in any other way than described in the
user documentation.
WARNING
Use of this equipment in other ways than those specified in the
user documentation may result in physical damage because of
exposure to irradiated light or electric shock or injury from touching
an operating part.
20 Amersham Imager 680 Operating Instructions 29271308 AB
2 Safety instructions
2.1 Safety precautions