Kent Scientific CODA User manual

CODA Monitor
User’s Guide

©2017 Kent Scientific Corporation, Documentation Version 1.0
for On-Unit Help Version 1.01 and Firmware Revision 1.01.00 March 2017
CODA Monitor
User’s Guide

TABLE OF CONTENTS
Introduction ......................................................................... 1
System Components ....................................................................1
About the CODA® Monitor ........................................................ 2
Navigating the CODA Monitor.................................................. 2
Using the CODA Monitor
Non-Invasive Blood Pressure ........................................................... 5
Set Up Hardware .......................................................................... 5
Configure Settings....................................................................... 5
Connect the Animal.....................................................................6
CODA Tips ..................................................................................... 8
Troubleshooting ...........................................................................9
CODA Cu and Bladder Maintenance ...................................12
RightTemp®
Temperature Monitor and Homeothermic Warming .................13
Set Up Hardware .........................................................................13
Configure Settings......................................................................13
Monitoring an Animal................................................................ 14
Troubleshooting ..........................................................................15
RightTemp Pad and Sensor Maintenance .............................15
System Customization.....................................................16
Profiles.......................................................................................... 16
Custom Dial Functions.............................................................. 16
Alarms............................................................................................18
TOC Continued ...

Data Management ...........................................................20
Connecting to a PC................................................................... 20
Upload ......................................................................................... 20
History .......................................................................................... 21
Analog Output............................................................................23
CODA PC Software.......................................................... 24
Install CODA Software ..............................................................24
Use CODA Software ..................................................................25
General Information ........................................................ 26
Contact ........................................................................................26
Warranty and Satisfaction Guarantee...................................26
Specifications .............................................................................27
Index ................................................................................... 29

1
Introduction
The CODA Monitor is not designed, intended, or authorized for use in human applications.
System Components
Front Panel
Back Panel
Dial Controls
Touch Screen
Display
VPR Port
O-Cu Port
RightTemp Module
• Pad Power
• Animal Sensor
• Pad Sensor

2
About the CODA Monitor
The Method
An occlusion tail cu is inflated to impede blood flow to an animal’s tail. The cu is
deflated slowly and a second tail cu, incorporating the VPR sensor, measures the
physiological characteristics of the returning blood flow. As the blood returns to the
tail, the VPR sensor cu measures the tail swelling that results from arterial pulsations
from the blood flow. Systolic blood pressure is measured automatically at the first
appearance of tail swelling. Diastolic blood pressure is measured automatically when the
rate of swelling stops increasing in the tail.
Navigating the CODA Monitor
Touch Screen
CODA Monitor Touch Screens oer quick access to features and settings. Touch the
upper half of the Run Screen to reach the first Touch Screen.
Run
Screen
Power
Off
General
Setup
Help
Start
CODA
Animal
Type
Interval Deflaon
Upload
Format
CODA
Tests
First Touch Screen
(Screen buttons may vary based on the modules installed.)

3
Touch the bottom half of the Run Screen to open the pop-up Display Menu and quickly
scroll through charts, view other data or silence Alarms.
BP Cycle - OCuff -VPR
Mean BP
-- mmHg
Diastolic BP
-- mmHg
Heart Rate
-- bpm
MeasRR
133 bpm
Systolic BP
-- mmHg
Pad
degC
Stop Alarm
Chart1
Param
Hist Chart2
STOPPED
Pop-Up Display Menu
(Screen buttons may vary based on the modules installed.)
Dial Control
There are two Dials, A and B. The function of a Dial varies based on:
• where you use it – from the Touch Buttons Screens, Run Screen,
Menu Screens or Main Menu.
• how you use it – turn, press or hold down.
Dials can have specialized uses in a few specific locations. The unit displays on-screen
instructions in those locations.
ACTION DIAL RESULT
Press A
B
Back one level from Main Menu Screens
To Run Screen from Main Menu
To Run Screen from Touch Button Screens
Select a highlighted Menu item
To Main Menu from Home Screen
Clear Alarms if they are enabled
Move through Menu choices
Change setting values at Menus
A
B
Turn
A
B
—
To Main Menu from Run Screen
To Help at any Menu screen
Hold
down
See Main Menu
on page 4.

4
Display Options
Customizing the Run Screen Display:
To configure Parameters:
1. Touch:
1) the upper half of the Run Screen for the Touch Screen Menu.
2) “General setup”.
3) “Config”.
4) “Param Locations”.
2. Turn Dial A to choose a Parameter and Dial B to assign the Parameter to a
Run Screen location.
To configure Charts:
1. Touch:
1) the upper half of the Run Screen for the Touch Screen Menu.
2) “General setup”.
3) “Config”.
4) “Chart 1 setup” or “Chart 2 setup” to choose the type and timing for a chart.
2. Press Dial A to return to the General setup page of the Touch Screen Menu.
3. Touch “Set ranges” to choose the best-fitting axis range for the Parameters
on your charts.
Menus
The Main Menu is the non-touch gateway to all features and setting choices for
configuring the CODA Monitor, useful when a touch screen is not a feasible option, such
as for an operator with damp hands. Access the Main Menu through the Touch Screen
Menu or from the Run Screen by holding Dial A.
Main Menu
CODA
RightTemp
Data mgmt
Config
Power off
Help
Go to CODA Menu
Press Dial B for the CODA
Menu. Configure your blood
pressure tesng procedure.
Hold Dial A for Help.
Main Menu
(Screen buttons may vary based on the modules installed.)

5
CODA Monitor
Set Up Hardware
Cuffs:
1. Attach the VPR cu to the VPR port.
2. Attach the Occlusion cu to the O-Cu port.
Configure Settings
CODA Monitor Settings
1. Touch:
1) the upper half of the Run Screen for the Touch Screen Menu.
2) “Animal Type” to select the animal species.
3) “Interval” to set the time between measurement cycles.
4) “Deflation” to choose how long the cu remains inflated.
Tip: Select 15 seconds for large rats
and 20 seconds for mice or rat pups.

6
Connect the Animal
1. Place the animal in the holder if you are using it:
1) Remove the rear gate of the animal holder.
2) Adjust the nose cone to the front of the holder and tighten the screw.
3) Place the animal into the holder. Allow the animal to enter the holder freely,
using as little force as possible.
4) Replace the rear gate, ensuring that the animal’s tail extends beyond the end
of the hatch.
5) Adjust the nose cone so that the animal appears comfortable but movement
is limited.
2. Place the animal on the infrared warming pad.
Warming is important! Remember to
set up your RightTemp. See page 13.
Tip: Make sure the entire length of
the animal’s tail rests on the warming
pad.
3. Position the cus on the animal’s tail:
• Place the O-Cu first so that it is near the base of the tail. It should slide freely,
but fit closely.
Slide the O-Cuff towards the base of the tail
until you begin to encounter resistance. Do not
force the cuff onto the tail.
A properly fitting cuff moves freely and fits
firmly during inflation. A cuff that is too tight
compresses underlying blood vessels, resulting
in poor blood pressure readings.
If a cuff feels tight or cannot reach the tail base,
try using a larger O-Cuff. If the cuff slides up the
entire length of the tail or fits loosely, try using
a smaller O-Cuff.

7
• Slide the VPR cu up the tail, with the larger diameter end first, until you reach
the O-cu.
O-Cu placed on tail
Do not force the cuff onto the tail.
Measurements can be detected
from any part of the tail as long as a
sufficient portion of the tail is inside
the VPR cuff.
VPR and O-Cu placed on tail
4. Before you begin blood pressure measurements, measure the temperature
at the base of the tail with the provided non-contact infrared thermometer. The
tail temperature should be between 32 and 35C.

8
Note: Do not start the experiment if the tail temperature
is below 32C. It may take several minutes for the animal’s
tail to reach the proper temperature.
5. Touch “Start CODA” to begin measurements.
CODA Tips
CODA Tips
• Choose a warm, quiet room for blood pressure measurements.
Avoid high-trac or noisy locations.
• Blood pressures may vary throughout the day. If repeated measurements
are necessary, schedule them for approximately the same time each day.
• Bladders can deteriorate over time. To prolong the life of the cus and
bladders, store them in the provided storage case when not in use.
• If you are using an animal holder, ensure that the animal fits snugly.
If the animal appears overly constrained, adjust the nose cone or use
a larger-size holder.
• Training sessions may be beneficial for some animals.
o If training is required, we recommend three to four 15-minute
training sessions in the days prior to beginning your study.
• When you use the CODA in anesthetized animals, induce slowly and
at the lowest possible dose to minimize the depressant eects of the
anesthetic agents.
o We strongly recommend inhalant anesthetics at low doses.
• Cus should be neither too tight nor too loose. If the cus feel tight,
try using a larger size. Properly fitting cus will move freely and fit firmly
during inflation.
o The need for a larger O-Cu does not necessarily mean a larger
VPR sensor is required.
• Once the experiment has begun, refrain from manipulating the animals.
• Before beginning the experiment, verify that the animal’s tail temperature
is between 32C and 35C.
o Do not start the experiment if the animal’s tail temperature is
less than 32C.

9
CODA Troubleshooting
Problem Possible Cause Solutions
No measurements
are recorded.
Measurements are
significantly higher
than expected.
Cus are too tight. • Use a larger cu.
• Slide the cus down the tail until they are
no longer tight.
Tail temperature is too low. • Ensure that the base of the tail measures
at least 32C.
Cus are wrong size. • Ensure that the cus are not too loose or
too tight on the tail.
Cu bladders are leaking. • Replace the cu bladders.
Error Messages
Problem Possible Cause Solutions
Main Line
press error
CODA cannot reach
initial pressure.
• O-Cu and/or VPR
ports are open or not
connected to the cus.
• Cu or cu bladders
are leaking.
• Ensure the cus are connected tightly to
the CODA Monitor.
• See “Diagnostic Procedures” to diagnose
and determine the source of potential leak.
SBP & DBP
unknown
VPR curve fails Sig-
nature shape test.
SBP,DBP < 1sec
SP & DP separated
by less than 1 sec-
ond.
SBP,DBP > 1/2 cyc
SP & DP separated
by more than half
data collection time.
• Tail temperature is
too low.
• Animal is moving
during measurement.
• Cu bladders are
leaking.
• Cu size is too large or
too small.
• Ensure animal’s tail temperature measures
between 32C and 35C.
• Confirm that the cus are placed properly
on the tail.
• Ensure that the cus are not too loose or
too tight on the tail.
• See “Diagnostic Procedures” to diagnose
and determine the source of potential leak.
Tail volume too low • Tail temperature is
too low.
• Cu size is too large or
too small.
• Ensure animal’s tail temperature measures
between 32C and 35C.
• Ensure that the cus are not too loose or
too tight on the tail.

10
Sample Displays
Correct BP Curve
VPR Cu Leaks
Two sample displays showing leaking VPR cus. The top channel in each display shows
a nearly horizontal blue line, indicating no leak in the VPR cu. The bottom channel in
each display shows a sharp drop at the beginning of the cycle, indicating a probable
VPR cu leak. Notice the red line for the O-cu drops smoothly, indicating no leak in
the O-cu.
O-Cu Leaks

11
Diagnostic Procedures
To access these diagnostic procedures, touch “CODA Tests”.
Resulting
Message
Meaning Possible Action
Success
Fail
No leaks found. No action required.
Air Leak. Use O-Cu and VPR Cu tests to locate the cause of
the leak. If you are unable to pinpoint and correct the
problem, contact Kent Scientific.
Full Tests
Resulting
Message
Meaning Possible Action
Success
Fail
No leaks found. No action required.
Air Leak in O-cu or
bladder.
Replace the bladder within the cu and test again. If
you are unable to correct the problem, contact Kent
Scientific.
O-Cuff Test
Resulting
Message
Meaning Possible Action
Success
Fail
No leaks found. No action required.
Air Leak in VPR cu
or bladder.
Replace the bladder within the cu and test again. If
you are unable to correct the problem, contact Kent
Scientific.
VPR Test

12
Bladders in both the O-Cus and VPR cus require periodic replacement. Replacement
bladders are included with your CODA Monitor.
CODA Cu and Bladder Maintenance
Bladder shelf life is approximately a year. Use only fresh
Kent Scientific pre-measured cuff-bladder replacements.
Other materials or replacement bladders could seriously
affect blood pressure readings.
1. Remove the external rubber washers (O-Rings) that secure the bladder at
each end.
2. Slide the old bladder out through either end of the cu and discard it.
3. Insert the new bladder into the cu.
4. Fold the bladder over each end of the cu.
5. Secure the bladder to the cu by sliding the O-ring over the ends and toward
the middle of the cu. Be careful not to overstretch the bladder material.
Prolong the life of your cuffs and bladder by storing
them in an air tight container when not in use.

13
RightTemp Temperature Monitor
and Homeothermic Warming
Set Up Hardware
1. Connect the warming pad to the “Pad Power” port on the front of the
CODA Monitor.
2. Choose one of the two probes as the body temperature sensor.
• For mice, select the smaller probe.
• For rats, select the larger probe.
3. Connect the body temperature sensor to the “Animal Sensor” port.
4. Use the other probe as the pad sensor.
5. Connect the sensor to the “Pad Sensor” port.
We recommend that you secure the pad sensor to the warming
pad using tape or something similar.
Configure Settings
RightTemp Sensor Calibration
1. Place both sensors in a beaker of water and measure the water temperature with
an accurate digital thermometer.
2. Wait for the screen display for the sensors to stabilize.
3. Touch:
1) “General setup”.
2) “RightTemp setup”.
3) “Calibrate temp”.
4. Turn Dial B to enter the temperature as measured by the digital thermometer.
5. Press Dial B to save the calibration.

14
RightTemp Settings
1. Touch:
1) “General setup”.
2) “RightTemp setup”.
3) “Control warming” to choose the type of warming:
• “O“: temperature monitoring, no warming
• “Unregulated“: temperature monitoring, warming with no sensor control
• “Pad temp regulated“: temperature monitoring with Pad sensor control
• “Body temp regulated”: temperature monitoring with Animal sensor control
2. Set the target temperature, limit the maximum pad temperature, or set the
power level depending on the type of warming you have selected. Appropriate
buttons will appear on the screen to allow you to make settings.
Monitoring an Animal
1. Place the anesthetized animal on the warming pad.
2. Insert the animal sensor into the rectum of the animal. Secure the sensor if
necessary.
Always secure the pad temperature sensor beneath the animal.
Anesthetized Animals
1. Place the animal holder directly on the warming pad.
2. Place both pad and body probes beneath the animal holder. Secure the sensors
if necessary.
Conscious Animals

15
Be sure to set warming control to Pad Temperature regulation,
as the rectal probe cannot be used in conscious animals.
RightTemp Troubleshooting
Problem Possible Cause Solutions
Pad is not heating. Settings are incorrect. • Ensure that all settings are properly enabled
for warming and that target temperatures
are selected.
Connections are loose or
incorrect.
• Ensure that the connection on the front
of the CODA Monitor is tight.
Pad is overheating.
Sensor placement is
incorrect.
• Place pad sensors directly beneath the
animal.
• Confirm animal sensor is properly placed
in rectum.
Settings are incorrect. • Ensure that all settings are properly enabled
for warming and that correct target
temperatures are selected.
• Enable a “Maximum Pad Temp” when type
of warming is “Body temp regulated” to
prevent overheating.
Sensors are
reading incorrect
temperatures.
Sensors require calibration. • Recalibrate the temperature sensors.
Sensor function has been
reassigned.
• Recalibrate the temperature sensors. Do this
any time you reassign sensor function.
RightTemp Pad and Sensor Maintenance
• Use the disposable warming pad covers to prevent soiling the warming pad.
• Wipe the warming pad clean with a damp cloth if needed. Never saturate the
warming pad.
• Gently wipe the sensors clean between uses.

16
System Customization
Profiles
To save your current settings as a new Prole:
1. Touch
1) the upper half of the Run Screen for the Touch Screen Menu.
2) “General setup”.
3) “Profiles”, then “Profiles Menu”.
2. Turn Dial B to select one of the available Profiles.
3. Press
1) Dial B and follow on-screen directions to edit the name of that Profile.
2) Dial B to save the Profile name.
4. Touch “>>”, then press Dial B to save current settings to the highlighted Profile.
CODA Monitor can save your settings as one or more Profiles. Profiles contain all
non-calibration-related settings, including display and functional settings.
To load a previously saved Prole:
Touch
1) the upper half of the Run Screen for the Touch Screen Menu .
2) “General setup”.
3) “Profiles”.
4) “Load” for the Profile you would like to load.
Custom Dial Functions
You can program the Dials to provide quick access to Parameter setting changes. This is
convenient in cases where you must frequently change a particular Parameter setting in
the middle of a procedure.
To assign a new dial function:
1. Touch:
1) the upper half of the Run Screen for the Touch Screen Menu.
2) “General setup”.
3) “Config”.
4) “Dial Menu”.